References on the Administration of Antibodies Using ALZET^® Osmotic Pumps

R0218 Miwa,T, Moriizumi,T, Horikawa,I, Uramoto,N., Ishimaru,T. Role of Nerve Growth Factor in the Olfactory System. Microscopy Res.and Tech. 2001; 58(--):197-203. >>> Antibody, anti-nerve growth factor; CSF/CNS (olfactory bulb); Mice; 2004; 28 days; ALZET pumps mentioned on pg. 200.

R0097 Nicoll,C.S., Liu,L., Alarid,E., Chiang,M., Russell,S.M. Analysis of the role of hormones and growth factors in growth control and tissue differentiation using transplanted mammalian embryos and fetal structures. Growth Reg. 1991; 1(--):133-144. >>> Antibody, anti-fibroblast growth factor; Antibody, anti-IGF I; Insulin; Growth hormone, ovine; Fibroblast growth factor; Epidermal growth factor; Insulin-like growth factor II; Antibody, anti-epidermal growth factor; IV (suprarenal); no duration posted; peptides.

P9199 Kupiec-Weglinski,J.W., Diamantstein,T, Tilney,N.L., Strom,T.B. Therapy with monoclonal antibody to interleukin 2 receptor spares suppressor T cells and prevents or reverses acute allograft rejection in rats. Proc Natl Acad Sci U S A 1986; 83(--):2624-2627. >>> Antibody, monoclonal, ART 18; Culture medium; IV (jugular); Rat; 2ML1; 5-10 days; Animal info (male, Sprague Dawley, 200-250 g.).

P9150 Sugiyama,S., Di Nardo,A.A., Aizawa,S., Matsuo,I., Volovitch,M., Prochiantz,A., Hensch,T.K. Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity. Cell 2008; 134(-3-):508-520. >>> Otx2, protein; antibody, inhibitory otx2; RNA, small interfering, penetratin coupled; CSF/CNS (visual cortex); Mice (transgenic); 1007D; 7 days; Controls received mp w/ vehicle; animal info (Otx2 KO); Otx2 is a homeoprotein.

P9138 Berhanu,D.A., Rush,R.A. Targeted silencing of TrkA expression in rat forebrain neurons via the p75 receptor. Neuroscience 2008; 153(-4-):1115-1125. >>> Immunoglobulin G, TrkA, inhibitor; immunoglobulin G, TrkA, scrambled; CSF/CNS (third ventricle); Rat; 2004; 7, 20 days; Controls received mp w/ TrkAm-fk Abp75; animal info (Sprague Dawley, adult, 270-300 g.); behavioral testing (Morris water maze apparatus).

P9120 Shen,Q., Wang,Y., Kokovay,E., Lin,G., Chuang,S.M., Goderie,S.K., Roysam,B., Temple,S. Adult SVZ stem cells lie in a vascular niche: A quantitative analysis of niche cell-cell interactions. Cell Stem Cell 2008; 3(-3-):289-300. >>> Antibody, GoH3; CSF, artificial; CSF/CNS; Mice (transgenic); 1007D; 6 days; Controls received mp w/ immunoglobulin G, rat; animal info (2-3 months old, Swiss Webster, GFAP-GFP).

P9109 Ng,B., Kramer,E., Liebes,L., Wasserheit,C., Hochster,H., Blank,E., Ceriani,R., Furmanski,P. Radiosensitization of tumor-targeted radioimmunotherapy with prolonged topotecan infusion in human breast cancer xenografts. Cancer Res. 2001/4/1; 61(-7-):2996-3001. >>> Antibody, monoclonal, BrE3; topotecan; SC; Mice (nude); 1007D; 14 days; Controls received no treatment; dose-response (fig. 3, pg. 2999); enzyme inhibitor (topoisomerase-1); cancer (breast); animal info (athymic, female, Swiss, 8-10 wks old); BrE3 antibody is a murine IgG1 monoclonal antibody.

P9090 Wannier-Morino,P., Schmidlin,E., Freund,P., Belhaj-Saif,A., Bloch,J., Mir,A., Schwab,M.E., Rouiller,E.M., Wannier,T. Fate of rubrospinal neurons after unilateral section of the cervical spinal cord in adult macaque monkeys: Effects of an antibody treatment neutralizing Nogo-A. Brain Research 2008; 1217(-;-):96-109. >>> Immunoglobulin G, monoclonal, mouse; antibody, anti-Nogo-A monoclonal, human; anitbody, anti-Nogo-A monoclonal, mouse; CSF/CNS (intrathecal); Monkey; 2ML2; ; Control animals received mp w/ control antibodies; animal info (macaque, 3-8 years old); pumps replaced every 2 weeks; polyethylene tubing used; spinal cord injury.

P8965 Matsuno,H., Yoshida,K., Ochiai,A., Okamoto,M. Requirement of methotrexate in combination with anti-tumor necrosis factor-α therapy for adequate suppression of osteoclastogenesis in rheumatoid arthritis. Journal of Rheumatology 2007; 34(-12-):2326-2333. >>> Influximab; immunoglobulin G, human; SC; Mice (SCID); 2 weeks; Controls received mp w/ human IgG; immunology; animal info (SCID-HuRAg-pit, 6-7 wks old; TNF-alpha inhibitor.

P8933 Fujii,T., Yonemitsu,Y., Onimaru,M., Inoue,M., Hasegawa,M., Kuwano,H., Sueishi,K. VEGF function for upregulation of endogenous PlGF expression during FGF-2-mediated therapeutic angiogenesis. Atherosclerosis 2008; 200(-1-):51-57. >>> Antibody, anti-VEGF; IP; Mice; 1007D; ; Ischemia (hind limb); animal info (male, C57BL/6, 6-7 wks old).

P8909 Liao,Y.J., Safa,P., Chen,Y.R., Sobel,R.A., Boyden,E.S., Tsien,R.W. Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-7-):2705-2710. >>> Immunoglobulin G, rabbit; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice; 1003D; 3 days; Functionality of mp verified w/ Alexa 488-labeled goat anti-rabbit IgG staining of coronal sections through the cerebellum); ALZET brain infusion kit 3 used; post op. care (buprenorphine); animal info (C57BL/6, > 20 g.); neurodegenerative (cerebellar ataxia); "multiple practice surgeries to infuse a low-molecular weight dye Fast Green confirmed consistent delivery of pump" (pg. 2709).

P8893 Brenneman,M.M., Wagner,S.J., Cheatwood,J.L., Heldt,S.A., Corwin,J.V., Reep,R.L., Kartje,G.L., Mir,A.K., Schwab,M.E. Nogo-A inhibition induces recovery from neglect in rats. Behavioural Brain Research 2008; 187(-2-):262-272. >>> Antibody, anti-Nogo-A monoclonal 7B12; antibody, anti-Nogo-A monoclonal 11C7; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ control IgG antibody; no stress (see pg. 264); good methods (pg. 264); post op. care ("clean, warm environment"); pumps removed after 21 days.

P8826 Kyoto,A., Hata,K., Yamashita,T. Synapse formation of the cortico-spinal axons is enhanced by RGMa inhibition after spinal cord injury. Brain Research 2007; 1186(-;-):74-86. >>> Antibody, anti-RgMa; Immunoglobulin G, rabbit; CSF/CNS (intrathecal); Rat; 2002; 2 weeks; Controls received mp w/ control rabbit IgG; animal info (female, Wistar, 200-250g., SCI); repulsive guidance molecule (RGMa) is a myelin-derived neurite outgrowth inhibitor.

P8820 Muellner,A., Gonzenbach,R.R., Weinmann,O., Schnell,L., Liebscher,T., Schwab,M.E. Lamina-specific restoration of serotonergic projections after Nogo-A antibody treatment of spinal cord injury in rats. European Journal of Neuroscience 2008; 27(-2-):326-333. >>> Antibody, anti-Nogo-A monoclonal 11C7; antibody, anti-Nogo A monoclonal 7B12; Immunoglobulin G, mouse; CSF/CNS (intrathecal); Rat; 2ML2; 2 weeks; Controls received mp w/ IgG; post op. care (Baytril); animal info (Lewis, 2 months old, 160-180g. SCI).

P8776 Baumann,M., Bartholome,R., Peutz-Kootstra,C.J., Smits,J.F.M., Struijker-Boudier,H.A.J. Sustained tubulo-interstitial protection in SHRs by transient losartan treatment: An effect of decelerated aging?. American Journal of Hypertension 2008; 21(-2-):177-182. >>> Losartan; hydralazine; antibody, anti-TGF-B1; antibody, anti-IgG-K1; SC; Rat; 2004; 4 weeks; Controls received mp w/ saline; cardiovascular; antihypertensive; animal info (male, Wistar-Kyoto, SHR, 4 wks old).

P8752 Marko,S.B., Damon,D.H. VEGF promotes vascular sympathetic innervation. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-6-):H2646-H2652. >>> Antibody, anti-VEGF (164 and 120); immunoglobulin G; PBS; Dye, Evans blue; SC; Rat; 1002; 4, 7, 14, 21 days; Controls received mp w/ control IgG; functionality of mp verified by distribution of dye within surgical area; cardiovascular; animal info (male, Sprague Dawley, denervated femor of artery).

P8746 Ma,Q.L., Harris-White,M.E., Ubeda,O.J., Simmons,M., Beech,W., Lim,G.P., Teter,B., Frautschy,S.A., Cole,G.M. Evidence of A beta and transgene-dependent defects in ERK-CREB signaling in Alzheimer's models. Journal of Neurochemistry 2007; 103(-4-):1594-1607. >>> Antibody, monoclonal anti-amyloid peptide B (1-15); immunoglobulin IgG2b; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ non-specific mouse IgG2b; animal info (Tg2576, Tg-; 12, 22 months old); neurodegenerative (Alzheimer's disease); "AB vaccine or anti-AB antibodies are still among the most promising treatments for AD." (p. 1605).

P8638 Mi,S., Hu,B., Hahm,K.M., Luo,Y., Hui,E.S.K., Yuan,Q.J., Wong,W.M., Wang,L., Su,H.X., Chu,T.H., Guo,J.S., Zhang,W.M., So,K.F., Pepinsky,B., Shao,Z.H., Graff,C., Garber,E., Jung,V., Wu,E.X., Wu,W. LINGO-1 antagonist promotes spinal cord remyelination and axonal integrity in MOG-induced experimental autoimmune encephalomyelitis. Nature Medicine 2007; 13(-10-):1228-1233. >>> Antibody, anti, LINGO-1; CSF/CNS (intrathecal); Rat; 36 days; Controls received mp w/ isotype control antibody; pumps replaced every 2-weeks, anti-LINGO-1 is an IgG1-isotype antibody.

P8617 Carro,E., Trejo,J.L., Busiguina,S., Torres-Aleman,I. Circulating Insulin-Like Growth Factor I Mediates the Protective Effects of Physical Exercise against Brain Insults of Different Etiology and Anatomy. Journal of Neuroscience 2001; 21(-15-):5678-5684. >>> Antibody, anti-IGF-I; serum, rabbit; insulin-like growth factor I, recomb.; Saline; SC; Rat; mice; 1002; 2004; 4-6 weeks; 14 days; Controls received mp w/ normal rabbit serum; long-term study; peptides; animal info (male, C57BL/6, 25 grams, male, wistar, 250-300 grams).

P8609 Tsai,S.Y., Markus,T.M., Andrews,E.M., Cheatwood,J.L., Emerick,A.J., Mir,A.K., Schwab,M.E., Kartje,G.L. Intrathecal treatment with anti-Nogo-A antibody improves functional recovery in adult rats after stroke. Experimental Brain Research 2007; 182(-2-):261-266. >>> Antibody, anti-Nogo-A monoclonal; antibody, immunoglobulin G 1, anti-wheat auxin; CSF/CNS (intrathecal, lumbar); Rat; 2ML2; 2 weeks; Controls received mp w/ IgG1 or no treatment; brain tissue distribution; animal info (male, Long-Evans, 300 grams, MCAO); ischemia (cerebral); "anti-Nogo-A immunotherapy delivered through the more accessible intrathecal route is an effective way to restore neurological function following stroke." (p. 265).

P8533 Morrey,J.D., Siddharthan,V., Olsen,A.L., Wang,H., Julander,J.G., Hall,J.O., Li,H., Nordstrom,J.L., Koenig,S., Johnson,S., Diamond,M.S. Defining limits of treatment with humanized neutralizing monoclonal antibody for West Nile virus neurological infection in a hamster model. Antimicrob.Agents Chemother. 2007/7; 51(-7-):2396-2402. >>> Antibody, monoclonal, hE16; CSF/CNS (pons); Hamster; 2001D; 24 hours; Controls received aCSF; functionality of mp verified by previous study with dye; comparison of IP injections vs. mp; post op. care (buprenorphine); animal info (female, Syrian golden, >7 weeks old); cannula placement confirmed with dye; E16 is anti-domain III of the West Nile Virus envelope protein.

P8525 Saito,H., Kojima,T., Takahashi,M., Horne,W.C., Baron,R., Amagasa,T., Ohya,K., Aoki,K. A tumor necrosis factor receptor loop peptide mimic inhibits bone destruction to the same extent as anti-tumor necrosis factor monoclonal antibody in murine collagen-induced arthritis. Arthritis and Rheumatism 2007; 56(-4-):1164-1174. >>> Peptide, WP9QY; antibody, monoclonal, anti-TNF alpha; PBS; DMSO; SC; Mice; 2001; 19 days; Controls received mp w/ vehicle; dose-response (fig. 1); pumps replaced every 7 days; half-life (p. 1182) "short;" peptides; animal info (male, DBA/1J, 6 weeks old, collagen induced arthritis); 10% DMSO.

P8515 Ishii,T., Muranaka,R., Tashiro,O., Nishimura,M. Chronic intracerebroventricular administration of anti-neuropeptide Y antibody stimulates starvation-induced feeding via compensatory responses in the hypothalamus. Brain Research 2007; 1144(--):91-100. >>> Antibody, rabbit, anti-neuropeptide Y; Saline; CSF/CNS; Mice; 2002; 13 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; ALZET brain infusion kit 3 used; animal info (male, ddy, 7 weeks old, 35-40 g).

P8447 Sheehan,J.J., Zhou,C., Gravanis,I., Rogove,A.D., Wu,Y.P., Bogenhagen,D.F., Tsirka,S.E. Proteolytic activation of monocyte chemoattractant protein-1 by plasmin underlies excitotoxic neurodegeneration in mice. Journal of Neuroscience 2007; 27(-7-):1738-1745. >>> Antibody, anti-MCP-1; serum, goat; immunoglobilin G1, mouse; monocyte chemoattractant protein-1 recomb.; antiplasmin, alpha2-; PBS; CSF/CNS (hippocampus); Mice; 1007D; 7 days; Peptides; animal info (C57BL/6, wt, MCP-1 -/-, tPA -/-, plg -/-, 20-25g); neurodegenerative.

P8423 Cuzzocrea,S., Ronchetti,S., Genovese,T., Mazzon,E., Agostini,M., Di Paola,R., Esposito,E., Muia,C., Nocentini,G., Riccardi,C. Genetic and pharmacological inhibition of GITR-GITRL interaction reduces chronic lung injury induced by bleomycin instillation. FASEB Journal 2007; 21(-1-):117-129. >>> Immunoglobulin G1, Fc-GITR; Immunoglobulin G1, Fc Fusion protein; Saline, nonpyrogenic; SC; Mice; 2001; 7 days; Controls received mp w/ vehicle, or Fc fusion protein; animal info (male, female, Sv129, 8-9 wk old, 22-24g.).

P8414 Ohab,J.J., Fleming,S., Blesch,A., Carmichael,S.T. A neurovascular niche for neurogenesis after stroke. Journal of Neuroscience 2006; 26(-50-):13007-13016. >>> Antibody, anti-mouse Tie2, biotin conjugated; antibody, anti-mouse Tie2 antiopoietin 1, biotin conjugated; angiopoietin 1; AMD 3100; stromal-derived factor 1B, biotin conjugated; stromal-derived factor 1B, recomb.; PBS; Mice (transgenic); 1007D; 3,5,7 days; Controls received mp w/ vehicle; dose-response (fig. 7); cardiovascular; peptides; animal info (C57BL/6, 2 months old); Middle cerebral artery occlusion; transient bilateral common carotid occlusion.

P8409 Prada,C.M., Garcia-Alloza,M., Betensky,R.A., Zhang-Nunes,S.X., Greenberg,S.M., Bacskai,B.J., Frosch,M.P. Antibody-mediated clearance of amyloid-beta peptide from cerebral amyloid angiopathy revealed by quantitative in vivo imaging. Journal of Neuroscience 2007; 27(-8-):1973-1980. >>> Antibody, anti-amyloid protein beta (10D5); antibody, anti-human tau (16B5); Dye, indocyanine green; CSF/CNS; Mice (transgenic); 2002; 14 days; Controls received mp w/ anti-Tau AB; comparison of single CNS injection vs. mp; animal info (Tg2576, 10-13 months old); neurodegenerative (Alzheimer's disease, CAA); permanent cranial window for serial imaging with multiphoton laser-scanning microscopy; "with continous infusion of anti-AB antibody, the clearance of CAA was more robust and continued throughout the duration of the treatment." (pg. 1978).

P8397 Park,J.H., Widi,G.A., Gimbel,D.A., Harel,N.Y., Lee,D.H.S., Strittmatter,S.M. Subcutaneous Nogo receptor removes brain amyloid-beta and improves spatial memory in Alzheimer's transgenic mice. Journal of Neuroscience 2006; 26(-51-):13279-13286. >>> Nogo-66 receptor (310) ecto-Fc, rat; immunoglobulin G, rat; PBS; SC; Mice (transgenic); 2004; 12 weeks; Controls received mp w/ rat IgG; long-term study; pumps replaced every 28 days; animal info (APPswe/PSEN-1delta E9, 7-10 months old); neurodegenerative (Alzheimer's disease).

P8364 Deng,R., Balthasar,J.P. Comparison of the effects of antibody-coated liposomes, IVIG, and anti-RBC immunotherapy in a murine model of passive chronic immune thrombocytopenia. Blood 2007; 109(-6-):2470-2476. >>> Antibody, MWReg30 antiplatelet; Saline, sterile; BSA; IP; Mice; 1007D; 7 days; Cardiovascular; animal info (female, Balb/C, 20g.); MWReg30-induced thrombocytopenia; "The continuous infusion of antiplatelet antibody may more closely resemble sustained production of antiplatelet antibodies in chronic cases of human ITP, and this may allow a more meaningful evaluation of experimental therapies." (pg. 2475).

P8322 Sakaguchi,M., Shingo,T., Shimazaki,T., Okano,H.J., Shiwa,M., Ishibashi,S., Oguro,H., Ninomiya,M., Kadoya,T., Horie,H., Shibuya,A., Mizusawa,H., Poirier,F., Nakauchi,H., Sawamoto,K., Okano,H. A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-18-):7112-7117. >>> Antibody, anti-galectin-1; immunoglobulin G, rabbit; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle or rabbit IgG; peptides; animal info (male, 1295J wt or galectin-1 -/-, 8 weeks old).

P8277 Shi,X., Semkova,I., Muether,P.S., Dell,S., Kociok,N., Joussen,A.M. Inhibition of TNF-alpha reduces laser-induced choroidal neovascularization. Experimental Eye Research 2006; 83(-6-):1325-1334. >>> Etanercept; infliximab; antibody, monoclonal rat anti-mouse/rat TNF-alpha; Water, sterile; PBS; IP; Mice; 2001; 7 days; Controls received mp w/ vehicle; animal info (female, C57BL/6J, 4-6 wks old, laser eye injury); fluorescein angiography; "we administered etanercept systemically via osmotic pumps to achieve a permanent therapeutic concentration of the drug for 7 days." (p. 1331).

P8266 Urushitani,M., bou Ezzi,S., Julien,J.P. Therapeutic effects of immunization with mutant superoxide dismutase in mice models of amyotrophic lateral sclerosis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-7-):2495-2500. >>> Antibody, mouse anti hSOD1; antibody, rabbit polyclonal anit-hSOD1-FITC; Saline; CSF/CNS; Mice; 2004; 16, 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; stability verified by Western Blot w/ residual volume AB after 18 days infusion; brain tissue distribution; animal info (G93A SOD1, 85 days old); neurodegenerative (ALS).

P8225 Kondo,N., Sekijima,T., Kondo,J., Takamatsu,N., Tohya,K., Ohtsu,T. Circannual control of hibernation by HP complex in the brain. Cell 2006; 125(-1-):161-172. >>> Antibody, polyclonal, anti-HP20c; immunoglobulin G, rabbit; PBS; CSF/CNS; Chipmunk; 2002; 2 weeks; Controls received mp w/ IgG; dose-response (fig. 6); animal info (male, tamias sibiricus, hibernating).

P8219 Guillin,O., Diaz,J., Carroll,P., Griffon,N., Schwartz,J.C., Sokoloff,P. BDNF controls dopamine D3 receptor expression and triggers behavioural sensitization. Nature 2001; 411(-6833-):86-89. >>> Immunoglobulin G-Trk B; immunoglobulin G, human; CSF/CNS (striatum); Rat; 2001; 7 days; Controls received mp w/ IgG; dose-response (fig 3); brain tissue distribution; animal info (6-OHDA lesioned, male, Wistar, 180-200 grams); neurodegenerative (Parkinson's disease); tissue perfusion (striatum).

P8208 Martens,T., Schmidt,N.O., Eckerich,C., Fillbrandt,R., Merchant,M., Schwall,R., Westphal,M., Lamszus,K. A novel one-armed anti-c-met antibody inhibits glioblastoma growth in vivo. Clinical Cancer Research 2006; 12(-20-):6144-6152. >>> Antibody, anti-c-Met, OA5D5; Histidine; CSF/CNS (intratumoral); Mice (nude); 2004; 12, 21, 28 days; Controls received mp w/ vehicle; cancer (glioblastoma); ALZET brain infusion kit 2 used; animal info (male, NMRI-nu/nu, 6-8 wks old); "Because the OA-5D5 antibody is a relatively large but stable molecule, convection-enhanced drug delivery (by mp) was ideally suited to deliver it in our orthotopic model." (p.6151); tissue perfusion (tumor).

P8136 Ishii,K., Nakamura,M., Dai,H.N., Finn,T.P., Okano,H., Toyama,Y., Bregman,B.S. Neutralization of ciliary neurotrophic factor reduces astrocyte production from transplanted neural stem cells and promotes regeneration of corticospinal tract fibers in spinal cord injury. Journal of Neuroscience Research 2006; 84(-8-):1669-1681. >>> Antibody, polyclonal goat anti-CNTF; immunoglobulin-G, goat; CSF/CNS (Intrathecal, subarachnoid space); Rat; 2002; 14 days; Controls received mp w/ IgG; brain tissue distribution; animal info (female, Sprague-Dawley, 250-300g. spinal cord lesioning, NSC transplant).

P8129 Heinrich,C., Nitta,N., Flubacher,A., Muller,M., Fahrner,A., Kirsch,M., Freiman,T., Suzuki,F., Depaulis,A., Frotscher,M., Haas,C.A. Reelin deficiency and displacement of mature neurons, but not neurogenesis, underlie the formation of granule cell dispersion in the epileptic hippocampus. Journal of Neuroscience 2006; 26(-17-):4701-4713. >>> Antibody, monoclonal, CR-50; immunoglobulin G, mouse; DMEM; CSF/CNS (hippocampus); Mice; 1007D; 7 days; Controls received mp w/ mouse IgG; ALZET brain infusion kit use; brain tissue distribution; animal info (male, C57BL/6, 8-10 wks old); Reelin-neutralizing antibody; DMEM is Dulbecco's modified eagle medium; tissue perfusion (hippocampus).

P8121 Fujii,T., Yonemitsu,Y., Onimaru,M., Tanii,M., Nakano,T., Egashira,K., Takehara,T., Inoue,M., Hasegawa,M., Kuwano,H., Sueishi,K. Nonendothelial mesenchymal cell-derived MCP-1 is required for FGF-2- mediated therapeutic neovascularization - Critical role of the inflammatory/arteriogenic pathway. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2006; 26(-11-):2483-2489. >>> Antibody, anti-VEGF; IP; Mice (nude); 1007D; ; Controls received mp w/ IgG; cardiovascular; ischemia (hind limb); animal info (male, C57BL/6, 6-7 wk. old; Balb/c nu/nu, 5 wk. old; C57BL/6, CCR2-/-).

P8112 Ji,B., Li,M., Wu,W.T., Yick,L.W., Lee,X., Shao,Z., Wang,J., So,K.F., Mccoy,J.M., Pepinsky,R.B., Mi,S., Relton,J.K. LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injury. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 33(-3-):311-320. >>> Immunoglobulin G1, human; LINGO-1-Fc, recomb. human; PBS; CSF/CNS (intrathecal, subarachnoid space); Rat; 2004; 4 weeks; Controls received mp w/ vehicle, or IgG1; replacement therapy (dorsal hemisection); peptides; post op. care (Buprenorphine/Buprenex, Ampicillin); animal info (female, Long Evans, 7 wk old).

P8071 Morrey,J.D., Siddharthan,V., Olsen,A.L., Roper,G.Y., Wang,H., Baldwin,T.J., Koenig,S., Johnson,S., Nordstrom,J.L., Diamond,M.S. Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters. Journal of Infectious Diseases 2006; 194(-9-):1300-1308. >>> Antibody, monoclonal, hE16; CSF, artificial; CSF/CNS (parenchyma); Hamster; 2001D; 24 hours; Controls received mp w/ vehicle, or saline; comparison of ip injections vs. mp; brain tissue distribution; animal info (female, Syrian golden, 7 wks. old, 116g., infected w/ West Nile Virus); hE16 is specific for WNV envelope protein domain III "this delivery system facilitated broad delivery of Ab into the brain, with highest levels near the site of cannulation." (p.1305); tissue perfusion (parenchyma).

P8045 Thompson,H.J., Marklund,N., LeBold,D.G., Morales,D.M., Keck,C.A., Vinson,M., Royo,N.C., Grundy,R., McIntosh,T.K. Tissue sparing and functional recovery following experimental traumatic brain injury is provided by treatment with an anti-myelin-associated glycoprotein antibody. European Journal of Neuroscience 2006; 24(-11-):3063-3072. >>> Antibody, anti-MAG mAb; CSF/CNS; 1003D; 3 days; ALZET brain infusion kit 1 used; brain tissue distribution; animal info (male, Sprague-Dawley, 350-400 grams); cannula secured via tissue adhesive; 35 mm long PE tubing used.

P8029 Dijkstra,S., Duis,S., Pans,I.M., Lankhorst,A.J., Hamers,F.P.T., Veldman,H., Baer,P.R., Gispen,W.H., Joosten,E.A.J., Geisert,EE Jr  Experimental Neurology 2006; 202(-1-):57-66. >>> Antibody, anti-CD81; immunoglobulin, G; PBS; CSF/CNS (intrathecal); Rat; 2002; 2 weeks; Post op. care (fluid therapy, 3.0 ml saline); animal info (adult, female, Wistar, 11 weeks old, 200-240 grams); spinal cord cannulation technique outlined (p. 58); PE tubing used; pump functionality measured incorrectly; x-ray image of pump/cannulation (p. 59); behavioral study.

P8028 Obata,K., Katsura,H., Sakurai,J., Kobayashi,K., Yamanaka,H., Dai,Y., Fukuoka,T., Noguchi,K. Suppression of the p75 neurotrophin receptor in uninjured sensory neurons reduces neuropathic pain after nerve injury. Journal of Neuroscience 2006; 26(-46-):11974-11986. >>> Antibody, anti-NGF; antibody, anti-P75NTR; K252a; DMSO; saline; CSF/CNS (intrathecal, lumbar); Rat; 2001; ; Controls received mp w/ saline; animal info (male, Sprague-Dawley, 200-250 grams); nerve injury; silastic tubing used; K252a in 50% DMSO; behavioral testing.

P7977 Yamanaka,H., Obata,K., Kobayashi,K., Dai,Y., Fukuoka,T., Noguchi,K. Alteration of the cell adhesion molecule L1 expression in a specific subset of primary afferent neurons contributes to neuropathic pain. European Journal of Neuroscience 2007; 25(-4-):1097-1111. >>> Immunoglobulin G, non-immune mouse; antibody, anti-L1-CAM; SB203580; Saline; CSF/CNS (intrathecal); Rat; 2001; 2002; ; Animal info (Male, Sprague-Dawley, 200-250 grams); silicone tubing, 0.64 mm O.D filled with 5.0 ul's of saline; inserted ~ 0.5 cm's into the intrathecal space; enzyme inhibitor (p38 MAP kinase).

P7940 Ambati,J., Gragoudas,E.S., Miller,J.W., You,T.T., Miyamoto,K., Delori,F.C., Adamis,A.P. Transscleral delivery of bioactive protein to the choroid and retina. Invest Ophthalmol.Vis.Sci. 2000/4; 41(-5-):1186-1191. >>> Immunoglobulin G, FITC-, rabbit; antibody, monoclonal mouse anti-ICAM-1; immunoglobulin, mouse IgG2a; Eye (superotemporal scleral surface, transscleral); Rabbit; 2001D; 2ML4; 3,5,13,20,28 days; 24 hours; Controls received mp w/ control Ab; functionality of mp verified by fluorescence in occular tissues and plasma mAb levels; stability verified by efficacy experiments, FITC linkage timing; half-life (p. 1188), 3 days; ALZET brain infusion kit used; animal info (dutch-belted, pigmented); "We have developed a minimally invasive transscleral drug delivery modality that can deliver therapeutic concentrations of bioactive proteins to the choroid and retina without significant systemic absorption or tissue damage." (P. 1186-87).

P7932 Zeinoaldini,S., Swarts,H.J.M., Van de Heijning,B.J.M. Effects of central infusion and immunoneutralization of growth hormone on the timing of puberty and plasma leptin levels in the female rat. REGULATORY PEPTIDES 2006; 134(-2-3-):158-163. >>> Growth hormone, recomb. human; immunoglobulin G, goat; growth hormone, anti-rat; Saline, sterile; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides; post op. care (analgesia, ibuprofen); animal info (female, Wistar, 45 grams).

P7870 Nakai,S., Matsunaga,W., Ishida,Y., Isobe,K., Shirokawa,T. Effects of BDNF infusion on the axon terminals of locus coeruleus neurons of aging rats. NEUROSCIENCE RESEARCH 2006; 54(-3-):213-219. >>> Brain-derived neurotrophic factor; antibody, anti-BDNF; brain-derived neurotrophic factor, biotin-labeled; immunoglobulin G; PBS; BSA; CSF/CNS (frontal cortex); Rat; 2002; 14 days; Controls received mp w/ vehicle, or IgG; dose-response (fig. 3); ALZET brain infusion kit used; brain tissue distribution; peptides; post op. care (enrofloxacin); animal info (male, F344/N, 13 month old, 19 month old); mp primed 3-6 hours in 37 celsius saline.

P7840 Choi,J., Credit,K., Henderson,K., Deverkadra,R., He,Z., Wiig,H., Vanpelt,H., Flessner,M.F. Intraperitoneal immunotherapy for metastatic ovarian carcinoma: Resistance of intratumoral collagen to antibody penetration. Clinical Cancer Research 2006; 12(-6-):1906-1912. >>> Immunoglobulin G, donkey anti-rabbit; radio-isotopes; Kreb's solution; bicarbonate; ringer's solution; sodium azide; 125I tracer; IV (jugular); Rat (nude); 2001; 5-7 days; Cancer (metastatic ovarian carcinoma); animal info (RNU-nude, athymic, 150-180g.).

P7814 Hata,K., Fujitani,M., Yasuda,Y., Doya,H., Saito,T., Yamagishi,S., Mueller,B.K., Yamashita,T. RGMa inhibition promotes axonal growth and recovery after spinal cord injury. Journal of Cell Biology 2006; 173(-1-):47-58. >>> Antibody, anti-rat RGMA; immunoglobulin G, rabbit; CSF/CNS (intrathecal); Rat; 2002; 2 weeks; Controls received mp w/ rabbit IgG; animal info (female, Wistar, 200-250g., spinal cord hemisection); repulsive guidance molecule (RGM).

P7812 Ma,Q.L., Lim,G.P., Harris-White,M.E., Yang,F.S., Ambegaokar,S.S., Glabe,C.G., Teter,B., Frautschy,S.A., Cole,G.M. Antibodies against beta-amyloid reduce A beta oligomers, glycogen synthase kinase-3 beta activation and tau phosphorylation in vivo and in vitro. Journal of Neuroscience Research 2006; 83(-3-):374-384. >>> Antibody, monoclonal, anti-amyloid protein, beta (1-15); immunoglobulin, IgG2b, mouse; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ mouse IgG2b; post op. care (marcaine); neurodegenerative (Alzheimer's disease); animal info (male, female, 11-12 month old).

P7776 Stoyanovitch,A.G., Johnson,M.A., Clifton,D.K., Steiner,R.A., Fraley,G.S. Galanin-like peptide rescues reproductive function in the diabetic rat. Diabetes 2005; 54(-8-):2471-2476. >>> Galanin-like peptide; antibody, anti-GALP; immunoglobulin G, goat; CSF, artificaial; CSF/CNS; Rat; 2002; 11 days; Controls received mp w/ vehicle, or IgG; functionality of mp verified; AlZET brain infusion kit 2 used; peptides; animal info (adult, male, Sprague-Dawley, 280-320g.); catheter filled with vehicle to allow 6.5 days delay treatment before agent; cannula placement confirmed.

P7653 Wiig,H., Gyenge,C.C., Tenstad,O. The interstitial distribution of macromolecules in rat tumours is influenced by the negatively charged matrix components. JOURNAL OF PHYSIOLOGY-LONDON 2005; 567(-2-):557-567. >>> Herceptin; immunoglobulin G, 1-; Radio-isotopes; 125I tracer; 131I tracer; IV (jugular); Rat; 2001; 5-7 days; Cancer (mammary); animal info (female, Sprague-Dawley, 208-255 g).

P7618 Park,J.H., Gimbel,D.A., GrandPre,T., Lee,J.K., Kim,J.E., Li,W.W., Lee,D.H.S., Strittmatter,S.M. Alzheimer precursor protein interaction with the Nogo-66 receptor reduces amyloid-beta plaque deposition. Journal of Neuroscience 2006; 26(-5-):1386-1395. >>> Nogo-66 receptor (310) ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS; Mice (transgenic); 2004; 56 days; Controls received mp w/ rat IgG; long-term study; pumps replaced after 28 days; Alzet Brain Infusion Kit 2 used; brain tissue distribution; cyanoacrylate adhesive; neurodegenerative (Alzheimer's disease) animal info (C57BL/6J, APPswe/PSEN or WT, 6-8 months old).

P7574 Yamashita,N., Tashimo,H., Ishida,H., Matsuo,Y., Arai,H., Nagase,H., Adachi,T., Ohta,K. Role of insulin-like growth factor-I in allergen-induced airway inflammation and remodeling. Cellular Immunology 2005; 235(-2-):85-91. >>> Antibody, anti-IGF-I, murine; immunoglobulin G, goat; Saline, physiological; SC; Mice; 2 weeks; Controls received mp w/ goat IgG; dose-response (fig 2); immunology; animal info (male, 10-12 wk old).

P7556 Liebscher,T., Schnell,L., Schnell,D., Scholl,J., Schneider,R., Gullo,M., Fouad,K., Mir,A., Rausch,M., Kindler,D., Hamers,F.P.T., Schwab,M.E. Nogo-A antibody improves regeneration and locomotion of spinal cord-injured rats. Annals of Neurology 2005; 58(-5-):706-719. >>> Antibody, monoclonal mouse; anti-rat Nogo-A (623-640); antibody, monoclonal mouse; anti-Nogo-A (1-979); antibody, monoclonal mouse; anti- wheat auxin; PBS; CSF/CNS (intrathecal); Rat; 2ML2; 2 weeks; Controls received mp w/ monoclonal anti-wheat auxin Ab; functionality verified by Ab concentrations in CSF; post op. care (baytril, analgesic); animal info (female, Lewis, spinal cord lesion).

P7553 Markus,T.M., Tsai,S.Y., Bollnow,M.R., Farrer,R.G., O'Brien,T.E., Kindler-Baumann,D.R., Rausch,M., Rudin,M., Wiessner,C., Mir,A.K., Schwab,M.E., Kartje,G.L. Recovery and brain reorganization after stroke in adult and aged rats. Annals of Neurology 2005; 58(-6-):950-953. >>> Antibody, anti-Nogo-A monoclonal 7B12; antibody, immunoglobulin G, mouse; CSF/CNS; Rat; 2ML2; 2 weeks; Controls received mp w/ mouse IgG or no treatment; ischemia (cerebral); animal info (3-4 months old, 25 months old).

P7449 Li,S.L., Saragovi,H.U., Nedev,H., Zhao,C.N., Racine,R.J., Fahnestock,M. Differential actions of nerve growth factor receptors TrkA and p75(NTR) in a rat model of epileptogenesis. MOLECULAR AND CELLULAR NEUROSCIENCE 2005; 29(-2-):162-172. >>> Peptides, NGF mimics; antibody, anti-NGF monoclonal, NGF30; immunoglobulin G; PBS; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle or control peptide or IgG; peptides; post op. care (penicillin, apo-sulfatrim); animal info (male Long-Evans, hooded); cannula placement verified with cresyl violet staining; peptides: c25-35, c30-35, c92-92, ss51 (control).

P7428 Kum,W.W.S, Laupland,K.B, Chow,A. Defining a novel domain of staphylococcal toxic shock syndrome toxin-1 critical for major histocompatibility complex class II binding, superantigenic activity, and lethality. Can.J.Microbiol. 1999; 46(--):171-179. >>> Toxin-1, TSS; antibody, monoclonal anti-TSST-1; antibody, monoclonal; Saline; SC; Rabbit; 7 days; Controls received mp w/ vehicle; immunology.

P7367 Lenzlinger,P.M., Shimizu,S., Marklund,N., Thompson,H.J., Schwab,M.E., Saatman,K.E., Hoover,R.C., Bareyre,F.M., Motta,M., Luginbuhl,A., Pape,R., Clouse,A.K., Morganti-Kossmann,C., McIntosh,T.K. Delayed inhibition of Nogo-A does not alter injury-induced axonal sprouting but enhances recovery of cognitive function following experimental traumatic brain injury in rats. Neuroscience 2005; 134(-3-):1047-1056. >>> Antibody, anti Nogo-A monoclonal 11C7; PBS; CSF/CNS; Rat; 2ML2; 14 days; Controls received mp w/ IgGAb; ALZET brain infusion kit 2 used; behavioral study; traumatic brain injury.

P7332 Gu,Z.Z., Cui,J., Brown,S., Fridman,R., Mobashery,S., Strongin,A.Y., Lipton,S.A. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia. Journal of Neuroscience 2005; 25(-27-):6401-6408. >>> Antibody, rabbit anti-pan laminin; serum, rabbit; PBS; BSA; CSF/CNS (cortex); Mice; 1007D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); MCA occlusion.

P7265 Ji,B.X., Li,M.W., Budel,S., Pepinsky,R.B., Walus,L., Engber,T.M., Strittmatter,S.M., Relton,J.K. Effect of combined treatment with methylprednisolone and soluble Nogo-66 receptor after rat spinal cord injury. European Journal of Neuroscience 2005; 22(-3-):587-594. >>> Nogo-66 receptor (27-310) ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS (intrathecal); Rat; 2004; 28 days; Controls received mp w/ vehicle rat IgG; functionality of mp verified by residual volume; stability verified by analysis of remaining mp contents; post op. care (buprenorphine/buprenex, ampicillin); spinal cord injury.

P7244 Obata,K., Katsura,H., Mizushima,T., Yamanaka,H., Kobayashi,K., Dai,Y., Fukuoka,T., Tokunaga,A., Tominaga,M., Noguchi,K. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury. Journal of Clinical Investigation 2005; 115(-9-):2393-2401. >>> SB203580; antibody, anti-nerve growth factor; oligodeoxynucleotide, antisense; oligodeoxynucleotide, FITC antisense; oligodeoxynucleotide, missense; DMSO; saline; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle, or sham surgery or mismatch ODN or contralateral no treatment; antisense (TRPA1); brain tissue distribution; 50% DMSO.

P7201 Perrin,F.E., Lacroix,S., viles-Trigueros,M., David,S. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration. Brain 2005; 128(--):854-866. >>> Antibody, anti-MCP-1/MIA-1a; antibody, anti-IL-1B; immunoglobulin G; CSF/CNS (sciatic nerve); Mice; 1007D; 7 days; Controls received mp w/ IgG; animal info (adult female, BALB/c).

P7190 Holmberg,J., Armulik,A., Senti,K.A., Edoff,K., Spalding,K., Momma,S., Cassidy,R., Flanagan,J.G., Frisen,J. Ephrin-A2 reverse signaling negatively regulates neural progenitor proliferation and neurogenesis. GENES&DEVELOPMENT 2005; 19(-4-):462-471. >>> Ephrin-A2-Fc; Ephrin-B2-Fc; EphA7-Fc; Immunoglobulin G, goat anti-human; Ephrin-B1-Fc; PBS; CSF/CNS; Mice; 1007D; 3 days; Controls received mp w/ Fc; peptides; animal info (adult,male, C57BL/6).

P7140 Canudas,A.M., Pezzi,S., Canals,J.M., Pallas,M., Alberich,J. Endogenous brain-derived neurotrophic factor protects dopaminergic nigral neurons against transneuronal degeneration induced by striatal excitotoxic injury. MOLECULAR BRAIN RESEARCH 2005; 134(-1-):147-154. >>> TrKB-IgG; immunoglobulin G, human; CSF/CNS (substantia nigra); Rat; 1002; 2-72 hours; Controls received mp w/ human IgG; peptides; mp filled with PBS connected to 10 cm of tubing filled with agent; mp primed 4 hours at 37 degrees celsius.

P7032 Huang,B.S., Van Vliet,B.N., Leenen,F.H.H. Increases in CSF [Na+] precede the increases in blood pressure in Dahl S rats and SHR on a high-salt diet. American Journal of Physiology-Heart and Circulatory Physiology 2004; 287(-3-):H1160-H1166. >>> Ouabain; antibody, fab fragment; gamma globulin; CSF, artificial; CSF/CNS; Rat; 2002; 7-8,14 days; Controls received mp w/ vehicle, or gammaglobulins; cardiovascular; fab fragments binding ouabain-like compounds (OLC) with high affinity.

P7023 Obata,K., Yamanaka,H., Kobayashi,K., Dai,Y., Mizushima,T., Katsura,H., Fukuoka,T., Tokunaga,A., Noguchi,K. Role of mitogen-activated protein kinase activation in injured and intact primary afferent neurons for mechanical and heat hypersensitivity after spinal nerve ligation. Journal of Neuroscience 2004; 24(-45-):10211-10222. >>> U0126; SB203580; FR167653; SP 600125; antibody, anti-NGF, sheep; DMSO, saline; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; enzyme inhibitors (MAPK kinase, p38, JNK); 50% DMSO used.

P6915 Poulaki,V., Joussen,A.M., Mitsiades,N., Mitsiades,C.S., Iliaki,E.F., Adamis,A.P. Insulin-like growth factor-I plays a pathogenetic role in diabetic retinopathy. American Journal of Pathology 2004; 165(-2-):457-469. >>> Wortmannin; ly294002; antibody, monoclonal aLR-3; SP600125; SN50; geldanamycin; IP; Rat; 2002; 7 days; Enzyme inhibitor (P1-3K, JNK); diabetes.

P6913 Apte,R.S., Barreiro,R.A., Duh,E., Volpert,O., Ferguson,T.A. Stimulation of neovascularization by the anti-angiogenic factor PEDF. INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE 2004; 45(-12-):4491-4497. >>> Pigment epithelium-derived growth factor; antibody, anti-PEDF; SC; Mice; 2001; 7 days; Dose-response (4492, 4495); peptides; PEDF; "...we decided to use a subcutaneously implanted pump to deliver PEDF to circumvent any effects on angiogenesis as a result of iatrogenic trauma." (p. 4492); antiangiogenesis.

P6908 Alonso,G., Galibert,E., Duvoid-Guillou,A., Vincent,A. Hyperosmotic stimulus induces reversible angiogenesis within the hypothalamic magnocellular nuclei of the adult rat: a potential role for neuronal vascular endothelial growth factor. BMC NEUROSCIENCE 2005; 6(--):U1-U19. >>> Antibody, anti-vascular endothelial growth factor; PBS; CSF/CNS (supraoptic nucleus); Rat; 1007D; 6 days; Schematic fig 9; 10 mm, 26 gauge cannula used.

P6902 Fouad,K., Schnell,L., Bunge,M.B., Schwab,M.E., Liebscher,T., Pearse,D.D. Combining Schwann cell bridges and olfactory-ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord. Journal of Neuroscience 2005; 25(-5-):1169-1178. >>> Chondroitinase ABC; antibody, immunoglobulin G, recomb. mouse; galactosidase; Saline; PBS; CSF/CNS (intrathecal, lumbar); Rat; 2002; 4 weeks; Comparison of IT injections vs. mp; pumps replaced at two weeks; post op. care (antibiotic); spinal cord injury; Recathco catheter used; behavioral study.

P6858 Lopez-Lopez,C., LeRoith,D., Torres-Aleman,I. Insulin-like growth factor I is required for vessel remodeling in the adult brain. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-26-):9833-9838. >>> Insulin-like growth factor I; antibody, anti-IGF I; serum, rabbit; SC; Mice; 2001; 2 weeks; 28 days; Controls received mp w/ saline or serum; comparison of microsphere injections vs. mp.

P6834 Hashiya,N., Jo,N., Aoki,M., Matsumoto,K., Nakamura,T., Sato,Y., Ogata,N., Ogihara,T., Kaneda,Y., Morishita,R. In vivo evidence of angiogenesis induced by transcription factor ets-1 - Ets-1 is located upstream of angiogenesis cascade. Circulation 2004; 109(-24-):3035-3041. >>> Antibody, anti-vascular endothelical growth factor; antibody, immunoglobulin G, rabbit; IP; Mice; ; Cardiovascular; ischemia.

P6822 Lee,J.K., Kim,J.E., Sivula,M., Strittmatter,S.M. Nogo receptor antagonism promotes stroke recovery by enhancing axonal plasticity. Journal of Neuroscience 2004; 24(-27-):6209-6217. >>> Antibody, NgR (310) Ecto-Fc; Immunoglobulin G, rat; PBS; CSF/CNS; Rat; 2ML4; 9 weeks; Controls received mp w/ rat IgG; long-term study; pumps replaced after 28 days; good methods (p. 6210); ALZET brain infusion kit 2 used; peptides; cyanoacrylate adhesive; ischemia (cerebral); Nogo-Nogo receptor (NgR); NgR (310) Ecto-Fc is an IgG fusion protein with a soluble ectodomain fragment of NgG; behavioral study.

P6821 Li,S.X., Liu,B.P., Budel,S., Li,M.W., Ji,B.X., Walus,L., Li,W.W., Jirik,A., Rabacchi,S., Choi,E., Worley,D., Sah,D.W.Y., Pepinsky,B., Lee,D., Relton,J., Strittmatter,S.M. Blockade of Nogo-66, myelin-associated glycoprotein, and oligodendrocyte myelin glycoprotein by soluble Nogo-66 receptor promotes axonal sprouting and recovery after spinal injury. Journal of Neuroscience 2004; 24(-46-):10511-10520. >>> NgR (310) Ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS (intrathecal); Rat; 2ML4; 4 weeks; Controls received mp w/ rat IgG; peptides.

P6798 Brussee,V., Cunningham,F.A., Zochodne,D.W. Direct insulin signaling of neurons reverses diabetic neuropathy. Diabetes 2004; 53(-7-):1824-1830. >>> Insulin, human; FITC-Insulin; Insulin-like growth factor I; antibody, polyclonal anti-rat insulin; dye, india ink; antibody, polyclonal anti-albumin; Saline; SC; CSF/CNS (intrathecal); Rat; 2, 4 weeks; Controls received mp w/ vehicle or anti-albumin Ab; functionality of mp verified by dye; replacement therapy (streptozotocin induced diabetes); india ink used to confirm catheter placement and intrathecal delivery.

P6786 Stein,T.D., Anders,N.J., DeCarli,C., Chan,S.L., Mattson,M.P., Johnson,J.A. Neutralization of transthyretin reverses the neuroprotective effects of secreted amyloid precursor protein (APP) in APPSW mice resulting in tau phosphorylation and loss of hippocampal neurons: Support for the amyloid hypothesis. Journal of Neuroscience 2004; 24(-35-):7707-7717. >>> Antibody, anti-transthyretin; immunoglobulin G, goat; CSF, artificial; CSF/CNS (hippocampus); Mice (transgenic); mice; 2002; ; Controls received mp w/ goat IgG; ALZET Brain Infusion Kit used; brain tissue distribution; cyanoacrylate adhesive; neurodegenerative disease (Alzheimer's).

P6776 Reynolds,J.L., Ignatowski,T.A., Sud,R., Spengler,R.N. Brain-derived tumor necrosis factor-alpha and its involvement in noradrenergic neuron functioning involved in the mechanism of action of an antidepressant. Journal of Pharmacology and Experimental Therapeutics 2004; 310(-3-):1216-1225. >>> Tumor necrosis factor, recomb. rat; tumor necrosis factor, recomb. rat inactivated; serum, rabbit; antibody, polyclonal rabbit anti-mouse TNF; CSF, artificial; albumin, rat; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle or heat-inactivated rr TNF; no stress (see pg. 1217-18); ALZET Brain Infusion Kit used.

P6775 Gawlick,U., Kranz,D.M., Schepkin,V.D., Roy,E.J. A conjugate of a tumor-targeting ligand and a T cell costimulatory antibody to treat brain tumors. Bioconjugate Chemistry 2004; 15(-5-):1137-1145. >>> Antibody, anti-CD28 Fab/Folate; gadolinium; PBS; CSF/CNS (intratumoral); Mice (transgenic); 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by MRI w/ gadolinium infusion; comparison of IV, SC, ICV injections vs. mp; cancer (brain, choroid plexus); brain tissue distribution; MRI; cyanoacrylate adhesive; "The strongest effect on tumor growth assessed of anti-CD28 fab/Folate." (p. 1143). Plastics One cannula used.

P6647 Caille,I., Allinquant,B., Dupont,E., Bouillot,C., Langer,A., Mueller,U., Prochiantz,A. Soluble form of amyloid precursor protein regulates proliferation of progenitors in the adult subventricular zone. Development 2004; 131(-9-):2173-2181. >>> Amyloid precursor protein-Fc, soluble; batimastat; immunoglobulin G, human; amyloid precursor like protein 2-Fc, soluble; oligonucleotide, antisense penetratin; AraC; Sodium chloride; saline; CSF/CNS; Mice; 1003D; 1007D; 3,6 days; Controls received mp w/ vehicle; antisense (APP, APLP2); brain tissue distribution; batimastat inhibits clearage of several membrane proteins; neurodegenerative disease (Alzheimer's).

P6633 Tsutsumi,N., Yonemitsu,Y., Shikada,Y., Onimaru,M., Tanii,M., Okano,S., Kaneko,K., Hasegawa,M., Hashizume,M., Maehara,Y., Sueishi,K. Essential role of PDGFR alpha-p70S6K signaling in mesenchymal cells during therapeutic and tumor angiogenesis in vivo - Role of PDGFR alpha during angiogenesis. Circulation Research 2004; 94(-9-):1186-1194. >>> Antibody, polyclonal anti-PDGF-AA; IP; Mice; 1007D; 2001; 7 days; Cancer; cardiovascular.

P6630 King,V.R., Phillips,J.B., Brown,R.A., Priestley,J.V. The effects of treatment with antibodies to transforming growth factor beta 1 and beta 2 following spinal cord damage in the adult rat. Neuroscience 2004; 126(-1-):173-183. >>> Antibody, anti-TGF-beta 1; antibody, anti-TGF-beta 2; Saline; CSF/CNS (intrathecal); Rat; 1007D; 1 week; Controls received mp w/ vehicle; comparison of fibronectin mats vs. mp; brain tissue distribution; TGF is transforming growth factor; FN mats and mp "showed a similar pattern of results." (p. 175).

P6614 Kodama,I., Niida,S., Sanada,M., Yoshiko,Y., Tsuda,M., Maeda,N., Ohama,K. Estrogen regulates the production of VEGF for osteoclast formation and activity in op/op mice. Journal of Bone and Mineral Research 2004; 19(-2-):200-206. >>> Antibody, anti-vascular endothelial growth factor; vascular endothelial growth factor; Flt-1/Fe chimera; SC; Mice; 1002; 2 weeks; Plasma levels taken.

P6609 Cheng,N., Brantley,D., Bin Fang,W., Liu,H., Fanslow,W., Cerretti,D.P., Bussell,K.N., Reith,A.D., Jackson,D., Chen,J. Inhibition of VEGF-dependent multistage carcinogenesis by soluble EphA receptors. NEOPLASIA 2003; 5(-5-):445-456. >>> EphA2-Fc, soluble receptor; EphA3-Fc, soluble receptor; immunoglobulin G; IP; Mice (transgenic); 2002; 2 weeks; Controls received mp w/ vehicle; drug plasma levels taken; comparison of IP bolus injections vs. IP mp; cancer (carcinoma); "...In comparison to bolus IP injection, continuous administration of angiogenesis inhibitors by an osmotic pump implanted IP resulted in a decrease of the required dosage and improved the effectiveness of therapy." p. 451.

P6598 Roonprapunt,C., Huang,W.C., Grill,R., Friedlander,D., Grumet,M., Chen,S.H., Schachner,M., Young,W. Soluble cell adhesion molecule L1-Fc promotes locomotor recovery in rats after spinal cord injury. Journal of Neurotrauma 2003; 20(-9-):871-882. >>> Immunoglobin, L1-Fc chimera; antibody, monoclonal, mouse IgM; PBS; CSF/CNS (intrathecal); Rat; 2002; 2 weeks; Controls received mp w/ vehicle, or mouse IgM Ab; no stress (see pg.876); stress/adverse reaction: (see pg.876) "One rat died in each group during the planned 12 week observation period."; immunology; brain tissue distribution; the chimera construct includes the extracellular domain of mouse L1 fused to the human Fc (hinge, CH2-CH3 region) and inserted into a PEE-14 vector (p. 872).

P6554 Hjortland,G.O., Garman-Vik,S.S., Juell,S., Olsen,O.E., Hirschberg,H., Fodstad,O., Engebraaten,O. Immunotoxin treatment targeted to the high-molecular-weight melanoma-associated antigen prolonging the survival of immunodeficient rats with invasive intracranial human glioblastoma multiforme. Journal of Neurosurgery 2004; 100(-2-):320-327. >>> Antibody, monoclonal 9.2.27 - pseudomonas exotoxin A; dye, evans blue; PBS; CSF/CNS (intratumoral); Rat (nude); 2001D; 24 hours; Controls received mp w/ PBS; cancer (glioma biopsy tumors); ALZET brain infusion kit used; brain tissue distribution; mp primed in sterile saline at 37 degrees Celsius for 4 hours; mp removed after 3 days.

P6540 Grossi,P.M., Ochiai,H., Archer,G.E., McLendon,R.E., Zalutsky,M.R., Friedman,A.H., Friedman,H.S., Bigner,D.D., Sampson,J.H. Efficacy of intracerebral microinfusion of trastuzumab in an Athymic rat model of intracerebral metastatic breast cancer. Clinical Cancer Research 2003; 9(-15-):5514-5520. >>> Herceptin; antibody, anti-Her2 monoclonal; radio-isotopes; Saline; 125I tracer; IP; CSF/CNS (intratumoral); Rat (athymic); 2ML1; 7 days; Tissue perfusion (tumor); herceptin serum levels (p. 5516); comparison of systemic IP mp vs. intracerebral mp infusion; cancer (breast); brain tissue distribution; radiolabeled herceptin (transtuzumab) was also infused intratumorally, peritumorally, and i.p to study tissue distribution.

P6527 Tan,J.H., Wang,H., Leenen,F.H.H. Increases in brain and cardiac AT1 receptor and ACE densities after myocardial infarct in rats. American Journal of Physiology-Heart and Circulatory Physiology 2004; 286(-5-):H1665-H1671. >>> Antibody, fab fragments; gamma globulin; CSF/CNS; Rat; 2002; 4 weeks; Pumps replaced every two weeks; 23 gauge cannula used.

P6502 Imbe,H., Okamoto,K., Kadoya,T., Horie,H., Senba,E. Galectin-1 is involved in the potentiation of neuropathic pain in the dorsal horn. Brain Research 2003; 993(-1-2-):72-83. >>> Antibody, anti-galectin-1, recomb. human; immunoglobulin G, rabbit; CSF/CNS (Intrathecal, subarachnoid space); Rat; 2002; 11 days; Controls received mp w/ rabbit IgG ab; "Intrathecal administration of anti-rhGAL-1 Ab significantly reduced mechanical hypersensitivity on the ipsilateral side at 3, 7, 10 days after SNI operation compared to that of control Ab." (p. 78).

P6451 Seki,S., Sasaki,K., Igawa,Y., Nishizawa,O., Chancellor,M.B., De Groat,W.C., Yoshimura,N. Suppression of detrusor-sphincter dyssynergia by immunoneutralization of nerve growth factor in lumbosacral spinal cord in spinal cord injured rats. Journal of Urology 2004; 171(-1-):478-482. >>> Antibody, anti-nerve growth factor; CSF, artificial; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; spinal cord injury; PE-10 used for catheterization.

P6440 Matsunaga,W., Shirokawa,T., Isobe,K. BDNF is necessary for maintenance of noradrenergic innervations in the aged rat brain. NEUROBIOLOGY OF AGING 2004; 25(-3-):341-348. >>> Brain-derived neurotrophic factor; BDNF antibody-; immunoglobulin G; PBS; CSF/CNS (frontal cortex); Rat; 2002; 13-15 days; Controls received mp w/ vehicle; post op. care (antibiotic for 5 days after operation); cannula secured by dental cementl; BDNF dissolved in PBS (pH 7.4); brain tissue distribution was measured by biotin-labeled BDNF.

P6434 Osicka,T.M., Strong,K.J., Nikolic-Paterson,D.J., Atkins,R.C., Jerums,G., Comper,W.D. Renal processing of serum proteins in an albumin-deficient environment: an in vivo study of glomerulonephritis in the Nagase analbuminaemic rat. NEPHROLOGY DIALYSIS TRANSPLANTATION 2004; 19(-2-):320-328. >>> Albumin; ficoll-70, polydisperse; transferrin; immunoglobulin G; radio-isotopes; 14C tracer; 3H tracer; SC; Rat; 2001; 7 days; Plasma levels taken; peptides.

P6384 Poulaki,V., Mitsiades,N., Kruse,F.E., Radetzky,S., Iliaki,E., Kirchhof,B., Joussen,A.M. Activin A in the regulation of corneal neovascularization and vascular endothelial growth factor expression. American Journal of Pathology 2004; 164(-4-):1293-1302. >>> Antibody, anti-activin A; activin A; PBS; IP; Mice; 1007D; 8 days; Controls received mp w/ vehicle or control antibody.

P6354 Harrington,A.W., Leiner,B., Blechschmitt,C., Arevalo,J.C., Lee,R., Moerl,L., Meyer,M., Hempstead,B.L., Yoon,S.O., Giehl,K.M. Secreted proNGF is a pathophysiological death-inducing ligand after adult CNS injury. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-16-):6226-6230. >>> Antibody, anti-nerve growth factor, neutralizing mouse; serum; immunoglobulin-G; penicillin; streptomycin; PBS; CSF/CNS (cortex); Rat; 2001; 1 week; Controls received mp w/ vehicle.

P6328 Cabelli,R.J., Shelton,D.L., Segal,R.A., Shatz,C.J. Blockade of Endogenous Ligands of TrkB Inhibits Formation of Ocular Dominance Columns. Neuron 1997; 19(--):63-76. >>> Immunoglobulin G, TrkA; immunoglobulin, TrkB; immunoglobulin G, TrkC; NT-4/5; NT-3; BSA; PBS, sterile; CSF/CNS (visual cortex); Cat; ferret; 2002; 2,3 weeks; 2 days; Controls received mp w/ vehicle, or IgG protein fusions unrelated to Trk; comparison of CSF/CNS injections vs. mp; stability verified by Ab immunostaining of brain sections; peptides; post op. care (7 days oral amoxicillin); 3 week infusions had a flow rate of approx. 0.33 ul/hr, "the reduced flow rate (~0.33 ul/hr) was achieved by coating ~1/3 of the surface area of the minipump with hot paraffin prior to filling") p. 74).

P6161 Marsh,D.R., Wong,S.T., Meakin,S.O., MacDonald,J.I.S., Hamilton,E.F., Weaver,L.C. Neutralizing intraspinal nerve growth factor with a trkA-IgG fusion protein blocks the development of autonomic dysreflexia in a clip-compression model of spinal cord injury. Journal of Neurotrauma 2002; 19(-12-):1531-1541. >>> Immunoglobulin G, Trka; Imunoglobulin G, human; CSF, artificial; CSF/CNS (intrathecal); Rat; 2ML2; 14 days; Controls received mp w/ IgG, human; post op. care (antibiotics and analgesics); catheter was perforated with a sealed end.

P5929 Namba,T., Koike,H., Murakami,K., Aoki,M., Makino,H., Hashiya,N., Ogihara,T., Kaneda,Y., Kohno,M., Morishita,R. Angiogenesis induced by endothelial nitric oxide synthase gene through vascular endothelial growth factor expression in a rat hindlimb ischemia model. Circulation 2003; 108(-18-):2250-2257. >>> Immunoglobulin G; antibody, anti-vascular endothelial growth factor; IP; Rat; 28 days; Cardiovascular; peptides; pump model not stated; ischemia (hind-limb).

P5836 Coesmans,M., Smitt,P.A.S., Linden,D.J., Shigemoto,R., Hirano,T., Yamakawa,Y., van Alphen,A.M., Luo,C.D., van der Geest,J.N., Kros,J.M., Gaillard,C.A., Frens,M.A., De Zeeuw,C.I. Mechanisms underlying cerebellar motor deficits due to mGluR1-autoantibodies. Annals of Neurology 2003; 53(-3-):325-336. >>> Antibody, Immunoglobulin G; Antibody, anti-metabotropic glutamate receptor I; CSF/CNS (gray matter); Mice; 1007D; 1 week; Controls received mp w/ IgG; 1 day recovery.

P5801 Shingo,T., Sorokan,S.T., Shimazaki,T., Weiss,S. Erythropoietin regulates the in vitro and in vivo production of neuronal progenitors by mammalian forebrain neural stem cells. J Neurosci 2001; 21(-24-):9733-9743. >>> Erythropoietin; antibody, rabbit anti-EPO neutralizing; rabbit IgG; Saline; albumin, mouse serum; rat serum; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; peptides; Erythropoietin (EPO) Recomb. Human, antibody&IgG were dissolved in 0.9% saline with 1 mg/ml mouse serum albumin; pump/cannula schematic (p. 9739 fig A).

P5745 Jin,K., Mao,X.O., Sun,Y, Xie,L., Greenberg,D.A. Stem cell factor stimulates neurogenesis in vitro and in vivo. J Clin Invest 2003; 110(-3-):311-319. >>> Stem cell factor; Antibody, anti-c-kit; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; SCF was human recombinant; some animals received both agents concurrently.

P5712 Fukaya,K., Hasegawa,M., Mashitani,T., Kadoya,T., Horie,H., Hayashi,Y., Fujisawa,H., Tachibana,O., Kida,S., Yamashita,J. Oxidized galectin-1 stimulates the migration of Schwann cells from both proximal and distal stumps of transected nerves and promotes axonal regeneration after peripheral nerve injury. Journal of Neuropathology and Experimental Neurology 2003; 62(-2-):162-172. >>> Antibody, mouse IgG; antibody, anti-Nogo-A monoclonal 7B12; PBS; CSF/CNS; Rat; 2ML2; 14 days; Controls received mp w/ control antibody or vehicle; antihypertensive; ALZET brain infusion kit used; ischemia (cerebral); "This study showed that pump-controlled delivery of a Nogo-A-specific antibody (7B12) initiated 24 hours after experimental stroke promoted improvement of long term neurologic outcome in normotensive and SHR rats." (p. 162); schematic of ICV infusion and timeline of protocol (p. 157).

P5564 Huang,B.S., Leenen,F.H. Both brain angiotensin II and "ouabain" contribute to sympathoexcitation and hypertension in Dahl S rats on high salt intake. Hypertension 1998/12; 32(-6-):1028-1033. >>> Losartan; antibody, fab fragments; gamma globulin; CSF/CNS; Rat; 2002; 4 weeks; Controls received mp w/ gamma globulins; pumps replaced after 2 weeks.

P5469 Seki,S., Sasaki,K., Fraser,M.O., Igawa,Y., Nishizawa,O., Chancellor,M.B., De Groat,W.C., Yoshimura,N. Immunoneutralization of nerve growth factor in the lumbosacral spinal cord reduces bladder hyperreflexia in spinal cord injured rats. Journal of Urology 2002; 168(-5-):2269-2274. >>> Antibody, anti-nerve growth factor; CSF, artificial; CSF/CNS (intrathecal); Rat; 2002; 11-14 days; Controls received mp w/ vehicle; peptides.

P5468 Ohara,K., Kusano,M. Anti-transforming growth factor-beta 1 antibody improves survival rate following partial hepatectomy in cirrhotic rats. HEPATOLOGY RESEARCH 2002; 24(-2-):174-183. >>> Antibody, anti-TGF-B1; PBS; SC; Rat; 1003D; 3 days; Controls received mp w/ vehicle; immunology; hepatic cirrhosis model.

P5452 Matsuoka,H., Kwon,S.S., Yazawa,Y., Barbieri,M., Yoo,T.J. Induction of endolymphatic hydrops by directly infused monoclonal antibody against type II collagen CB11 peptide. Annals of Otology Rhinology and Laryngology 2002; 111(-7-):587-592. >>> Antibody, anti-CB11 monoclonal; Ear (scala tympani); Guinea-pig; 2002; 14 days; Controls received mp w/ mouse serum; tissue perfusion (scala tympani).

P5382 Margotti,E., Covaceuszach,S., Tongiorgi,E., Cattaneo,A., Domenici,L. TRKB signalling controls the expression of N-methyl-D-aspartate receptors in the visual cortex. European Journal of Neuroscience 2002; 16(-6-):1067-1074. >>> Immunoglobulin G, TrkB; Saline; CSF/CNS (occipital cortex); Rat (neonate); 16 days; Controls received mp w/ vehicle; functionality of mp verified by TrkB-IgG immunohistochemistry in visual cortex; immunology; TrkB-IgG consists of the soluble form of the tirosine Kinase receptor engineered as an immunoadhesin by fusing it to the Fc portion of an IgG; 20-21 day old rats used; Fig. 2A (graphic of infusion site).

P5326 Greive,K.A., Osicka,T.M., Russo,L.M., Comper,W.D. Nephrotic-like proteinuria in experimental diabetes. American Journal of Nephrology 2003; 23(-1-):38-46. >>> Immunoglobulin G; Albumin, rat serum; Transferrin; 3H Tracer; PBS; 14C Tracer; Radio-isotopes; SC; Rat; 2001; 7 days; Functionality of mp verified by plasma protein levels; rat serum albumin labeled w/ 14C; IgG and transferrin labeled w/ 3H; multiple pumps per animal (2).

P5308 Huang,B.S., Kudlac,M., Kumarathasan,R., Leenen,F.H. Digoxin prevents ouabain and high salt intake-induced hypertension in rats with sinoaortic denervation. Hypertension 1999/10; 34(-4 Pt 2-):733-738. >>> Digoxin; Antibody, Fab fragments; Gamma globulin; SC; CSF/CNS; Rat; 2002; 2ML2; 12-14 days; Controls received mp w/ gamma globulin solution; peptides; antihypertensive; digoxin infused SC via 2ML2 pumps; some animals received ICV Fab fragments concomitantly via 2002 pumps.

P5281 Hermans,J.J., van Essen,H., Struijker-Boudier,H.A., Johnson,R.M., Theeuwes,F., Smits,J.F. Pharmacokinetic advantage of intrapericardially applied substances in the rat. J Pharmacol Exp.Ther 2002/5; 301(-2-):672-678. >>> Heparin; Fibroblast growth factor 2; Immunoglobulin G, rat; PBS; Albumin, rat serum; fluorescein; texas red; IV (jugular); intrapericardial; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality verified by measuring drug concentration in residual volume; plasma and pericardial fluid drug levels checked; comparison of bolus injection vs. mp; cardiovascular; DURECT-authored; rhFGF and rat serum albumin labeled w/ Texas red; rat IgG was labeled w/ fluorescein Isothiocyanate (FITC).

P5270 Suga,M., Maclean,A.A., Keshavjee,S., Fischer,S., Moreira,J.M., Liu,M. RANTES plays an important role in the evolution of allograft transplant- induced fibrous airway obliteration. Am.J Respir.Crit Care Med. 2000/11; 162(-5-):1940-1948. >>> Antibody, polyclonal anti-rat RANTES; Immunoglobulin G, rabbit; RANTES; Saline; PBS; BSA; Tracheal graft; Rat; 1007D; 2002;; 7, 14, 24 days; Controls received mp w/ vehicle; pumps replaced after 14 days; 14-day stability verified by RANTES ELISA (p.1944); Immunology; peptides; transplantation; RANTES is a chemokine; concentration of RANTES in mp measured before and after delivery (p.1945).

P5197 Masaki,I., Yonemitsu,Y., Yamashita,A., Sata,S., Tanii,M., Komori,K., Nakagawa,K., Hou,X., Nagai,Y., Hasegawa,M., Sugimachi,K., Sueishi,K. Angiogenic gene therapy for experimental critical limb ischemia: acceleration of limb loss by overexpression of vascular endothelial growth factor 165 but not of fibroblast growth factor-2. Circ.Res 2002/5/17; 90(-9-):966-973. >>> Antibody, anti-Vascular Endothelial Growth Factor; Immunoglobulin G; IP; Mice; 1007D; 7 days; Peptides; VEGF levels checked by ELISA; ischemia (limb).

P5196 El Haj,T., Poole,S., Farthing,M.J., Ballinger,A.B. Anorexia in a rat model of colitis: interaction of interleukin-1 and hypothalamic serotonin. Brain Res 2002/2/8; 927(-1-):1-7. >>> Antibody, anti-Tumor necrosis factor-a; Interleukin-1 receptor antagonist; Immunoglobulin G; Saline; CSF/CNS (paraventricular nucleus); CSF/CNS; Rat; 1007D; 2001; 7 days; Controls received mp w/ vehicle; functionality verified by checking residual volume; peptides; multiple pumps per animal (2); 1007D delivered IL-1 receptor antagonist or saline to paraventricular nucleus; 2001 pumps delivered anti-TNF-a antibody to PVN; bilateral cannulae used.

P5162 Tatagiba,M., Rosahl,S., Gharabaghi,A., Blomer,U., Brandis,A., Skerra,A., Samii,M., Schwab,M.E. Regeneration of auditory nerve following complete sectioning, and intrathecal application of the IN-1 antibody. Acta Neurochirurgica 2002; 144(--):181-187. >>> Antibody, monoclonal IN-1 Fab fragment; PBS; CSF/CNS (intrathecal); rat; 2001; 1 week; controls received mp w/ vehicle; functionality of mp verified by residual aspiration; peptides; mouse IgG used as control antibody; photos of pump and animal with implanted pump (p. 184).

P5087 Dufourcq,P., Couffinhal,T., Alzieu,P., Daret,D., Moreau,C., Duplaa,C., Bonnet,J. Vitronectin is up-regulated after vascular injury and vitronectin blockade prevents neointima formation. Cardiovascular Research 2002; 53(--):952-962. >>> Antibody, monoclonal anti-vitronectin; Antibody, monoclonal anti-B3 integrin; Antibody, monoclonal anti-avB5 integrin; PBS; Immunoglobulin G, non-immune; perivascular (carotid artery adventitia); rat; 7 days; controls received control immunoglobulin; tissue perfusion (corotid adventitia); cardiovascular; peptides.

P5022 Teeling,J.L., Jansen-Hendriks,T., Kuijpers,T.W., de Haas,M., van de Winkel,J.G.J., Hack,C.E., Bleeker,W.K. Therapeutic efficacy of intravenous immunoglobulin preparations depends on the immunoglobulin G dimers: studies in experimental immune thrombocytopenia. Blood 2001; 98(-4-):1095-1099. >>> Albumin, rat serum; Transferrin, rat; Immunoglobulin G, rabbit anti-rat; Ficoll, polydisperse;; Thimerosal; Radio-isotopes; 3H tracer; 14C tracer;; SC; rat; 2--1; ; controls received mp w/ vehicle; plasma levels of radioactive material; dose-response (table 2 p. 1164); good methods (p. 1161). 14C used for labelling albumin; Thimerosol in vehicle was .002% to inhibit bacterial growth; IgG; transferrin, and polydisperse ficoll labelled with 3H; Rats implanted with 1 or 2 pumps..

P5006 Hiraki,Y., Shukunami,C., Iyama,K., Mizuta,H. Differentiation of chondrogenic precursor cells during the regeneration of articular cartilage. OSTEOARTHRITIS AND CARTILAGE 2001; 9(--):S102-S108. >>> Fibroblast Growth Factor 2; Antibody, beta-FM-1; Immunoglobulin G;; Saline; Knee (articular cavity); rabbit; 2002; 2 weeks; Arthritis; controls received mp w/ vehicle; tissue perfusion (knee joint); peptides; Silastic tubing used; Pump placed SC in hind leg region; Antibody adminstered to block effects of FGF-2; IgG administered as antibody control;.

P4996 Umemura,M., Nishimura,H., Hirose,K., Matsuguchi,T., Yoshikai,Y. Overexpression of IL-15 in vivo enhances protection against Mycobacterium bovis Bacillus Calmette-Guerin infection via augmentation of NK and T cytotoxic 1 responses. Journal of Immunology 2001; 167(--):946-956. >>> Antibody, monoclonal anti-IFN-Y; Immunoglobulin G, rat; SC; mice; 2004; 28 days; controls received mp w/ vehicle; functionality of mp verified by serum IFN-Y levels; immunology; peptides.

P4802 Kryger,G.S., Kryger,Z., Zhang,F., Shelton,D.L., Lineaweaver,W.C., Buncke,H.J. Nerve growth factor inhibition prevents traumatic neuroma formation in the rat.. JOURNAL OF HAND SURGERY-AMERICAN VOLUME 2001; 26A(-4-):635-644. >>> Immunoglobulin G, TrkA; PBS;; CSF/CNS (sciatic nerve);; rat;; 2004;; 30 days;; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve); stress/adverse reaction: (see p. 643) foreign body reaction observed, but did not interfere with pump delivery; peptides; silicone tube used; TrkA-IgG is a nerve growth factor inhibitor; TrkA-IgG consists of the high affinity NGF receptor (TrkA) fused to an IgG heavy chain;.

P4794 Wiig,H., Tenstad,O. Interstitial exclusion of positively and negatively charged IgG in rat skin and muscle.. American Journal of Physiology-Heart and Circulatory Physiology 2001; 280(--):H1505-H1512. >>> Antibody, Immunoglobulin, G1; Antibody, Immunoglobulin, G4;; Radio-isotopes; 125I tracer; 131I tracer;; IV (jugular);; rat;; 2001;; 120 - 168 hours;; Functionality of mp verified by plasma levels (Fig 1 p. H1507); peptides; IgG's were radioactive tracers;.

P4780 Dillard,D.G., Gal,A.A., Roman-Rodriguez,J., White,S., Jacobs,I.N. Transforming growth factor and neutralizing antibodies in subglottic stenosis.. Annals of Otology Rhinology and Laryngology 2001; 110(--):393-400. >>> Transforming growth factor-B1; Antibody, anti-human TGF-B1;; PBS; BSA;; Larynx;; rat;; 1, 7, 21 days;; Controls received mp w/ vehicle; immunology; peptides; special delivery device attached to pump, p. 394; wound healing study;.

P4772 Fedele,M.J., Lang,C.H., Farrell,P.A. Immunization against IGF-I prevents increases in protein synthesis in diabetic rats after resistance exercise. Am.J.Physiol.Endocrinol Metab. 2001; 280(--):E877-E885. >>> Antibody, anti-IGF-I polyclonal;; Saline;; SC;; rat;; 2ML1;; 7 days;; Controls received mp w/ non-specific Immunoglobulin G; functionality of mp verified by plasma levels; immunology; peptides; rats underwent partial pancreatetomy to create diabetic state; antibody delivered wan as anti-insulin-like growth factor I antibody; diabetes; pumps used for passive immunization against IGF-I; anti IGF-I antibody used is called SC-7144;.

P4734 Brosamle,C, Huber,A.B, Fiedler,M, Skerra,A, Schwab,M.E  The Journal of Neuroscience 2000; 20(-21-):8061-8068. >>> Antibody, monoclonal IN-1 - Fab fragment; Antibody, monoclonal mutant IN-1 - Fab fragment;; BSA;; CSF/CNS (intrathecal);; rat;; 2002;; 14 days;; Controls received mp w/ vehicle; immunology; peptides;.

P4715 Gu,Z, Toliver-Kinsky,T, Glasgow,J, Werrbach-Perez,K, Perez-Polo,J.R  Int.J.Devl.Neuroscience 2000; 18(--):455-468. >>> Antibody, anti-nerve growth factor;; PBS;; CSF/CNS;; rat;; 2002;; 2 weeks;; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit used; peptides; neurodegenerative (Alzheimer's disease).

P4638 Buchsbaum,D.J., Liu,T., Roberson,P.L., Bright,S, Elliott,D.G, Khazaeli,M.B. Comparison of biodistribution of 131I CC49 mAb in athymic nude mice bearing LS174T colon cancer xenografts following bolus injection or continuous infusion.. Tumor Targeting 1998; 3(--):178-190. >>> Antibody, monoclonal, CC49;; Radio-isotopes 131I; PBS; Albumin, human serum;; SC; IP;; mice (nude);; 2001;; 7 days;; controls received mp w/vehicle; functionality verified by (incorrectly) weighing pumps at the end of infusion; comparison of bolus IP injections vs. mp; cancer;.

P4552 Nair,T.S, Prieskorn,D.M., Miller,J.M., Dolan,D.F., Raphael,Y, Carey,T.E. KHRI-3 monoclonal antibody-induced damage to the inner ear: antibody staining of nascent scars.. Hearing Research 1999; 129(--):50-60. >>> Antibody, KHRI-3 monoclonal;; Medium, serum free;; ear (cochlea);; guinea-pig;; 2002;; 14 days;; controls received mp w/vehicle; tissue perfusion (scala tympani); immunology;.

P4525 Iversen,P.O, Lewis,I.D, Turczynowicz,S, Hasle,H., Niemeyer,C, Schmiegelow,K, Bastiras,S, Biondi,A, Hughes,T.P, Lopez,A.F  Blood 1997/12/15; 90(-12-):4910-4917. >>> Colony-stimulating factor, GM; Antibody, monoclonal anti-TNFa;; Saline, isotonic;; IP;; mice (nude);; 4 weeks;; controls received mp w/vehicle; functionality of mp verified by plasma levels; immunology; peptides;.

P4463 Lee,H.-M, Takeyama,K, Dabbagh,K, Lausier,J.A, Ueki,I.F, Nadel,J.A  Am.J.Physiol.(Lung Cell.Mol.Physiol.) 2000; 278(--):L185-L192. >>> Antibody, anti-tumor necrosis factor-a;; Saline;; SC;; rat;; 2ML1;; ; peptides;.

P4397 Burne,M.J., Osicka,T.M., Comper,W.D. Fractional clearance of high molecular weight proteins in conscious rats using a continuous infusion method;. Kidney International 1999; 55(--):261-270. >>> Albumin; Transferrin; Lactoperoxidase; Immunoglobulin G; Lactate dehydrogenase; Glucose oxidase;; 3H tracer; Radio-isotopes; Thimerisol;; SC;; rat;; 2001;; 7 days;; functionality of mp verified by plasma levels; peptides;.

P4394 Giehl,K.M., Schutte,A., Mestres,P., Yan,Q. The survival-promoting effect of glial cell line-derived neurotrophic factor on axotomized corticospinal neurons in vivo is mediated by an endogenous brain-derived neurotrophic factor mechanism. Journal of Neuroscience 1998/9/15; 18(-18-):7351-7360. >>> Brain-derived neurotrophic factor; Glial-derived neurotrophic factor; NT-3; Immunoglobulin G, anti-turkey; Antibody, rabbit affinity-purified BDNF-neutralizing-;; PBS; Penicillin; Streptomycin;; CSF/CNS (parenchyma);; rat;; 2001;; 7 days;; controls received mp with vehicle; tissue perfusion (cortical lesion); peptides; human recomb. GDNF&NT-3 used;.

P4390 Ignatowski,T.A., Covey,W.C., Knight,P.R., Severin,C.M., Nickola,T.J., Spengler,R.N. Brain-derived TNFa mediates neuropathic pain. Brain Research 1999; 841(--):70-77. >>> Antibody, monoclonal hamster anti-murine TNFalpha; Antibody, polyclonal rabbit anti-mouse TNFa; Tumor necrosis factor-a;; CSF, artificial; Gentamycin; Albumin, rat;; CSF/CNS;; rat;; 2002;; 14 days;; controls received mp with aCSF, normal rabbit serum or Armenian hamster IgG isotype control antibody; stability of each antibody, control, and rrTNFa verified by bioassay following 14 days of incubation in aCSF at 37ºC; peptides; ALZET brain infusion kit used; pain;.

P4387 Fagan,A.M., Suhr,S.T., Lucidi-Phillipi,C.A., Peterson,D.A., Holtzman,D.M., Gage,F.H. Endogenous FGF-2 is important for cholinergic sprouting in the denervated hippocampus. Journal of Neuroscience 1997/7/1; 17(-7-):2499-2511. >>> Antibody, anti-fibroblast growth factor 2;; CSF, artificial;; CSF/CNS;; rat;; 2002;; 2 weeks;; controls received mp with normal rabbit serum or aCSF; peptides;.

P4349 Rasika,S., Alvarez-Buylla,A., Nottebohm,F. BDNF mediates the effects of testosterone on the survival of new neurons in an adult brain. Neuron 1999; 22(-1-):53-62. >>> Brain-derived neurotrophic factor; AB1513P, BDNF antibody-; Saline; BSA;; CSF/CNS (high vocal center); Bird (canary); 1007D; 10 days; Controls received mp with vehicle; peptides; flow rate of mp reduced by immersion in molten paraffin; recomb. human BDNF used; schematic of experimental model (p. 55); small bird.

P4347 Lara,H.E., Dissen,G.A., Leyton,V., Paredes,A., Fuenzalida,H., Fiedler,J.L., Ojeda,S.R. An increased intraovarian synthesis of nerve growth factor and its low affinity receptor is a principal component of steroid-induced polycystic ovary in the rat. Endocrinology 2000; 141(-3-):1059-1072. >>> Antibody, nerve growth factor, polyclonal; Oligonucleotide, antisense phosphothionate;; Intraovarian;; rat;; 2ML4;; 56 days;; controls received mp with preimmune serum; tissue perfusion (bursa of ovary); long-term study, pumps replaced after 28 days; peptides; antisense; agents infused from same pump;.

P4344 Pizzorusso,T., Berardi,N., Rossi,F.M., Viegi,A., Venstrom,K., Reichardt,L.F., Maffei,L. TrkA activation in the rat visual cortex by antirat trkA IgG prevents the effect of monocular deprivation. European Journal of Neuroscience 1999; 11(--):204-212. >>> Immunoglobulin G, antirat trkA; Immunoglobulin G, antip75NTR-;; Saline, sterile;; CSF/CNS (visual cortex);; rat;; 1007D; 1 week;; controls received mp with vehicle or control IgG; peptides; brain tissue distribution assessed with anti-rabbit IgG antibodies; RTA-IgG given singly and in same pump as antip75NTR IgG;.

P4320 Zhou,X.-F., Deng,Y.-S., Chie,E., Xue,Q., Zhong,J.-H., McLachlan,E.M., Rush,R.A., Xian,C.J. Satellite-cell-derived nerve growth factor and neurotrophin-3 are involved in noradrenergic sprouting in the dorsal root ganglia following peripheral nerve injury in the rat. European Journal of Neuroscience 1999; 11(--):1711-1722. >>> Antibody, nerve growth factor; Antibody, NT-3; Immunoglobulin, normal sheep;; CSF/CNS (dorsal root ganglia);; rat;; 2 weeks;; ; tissue perfusion (DRG via lesioned L5 spinal nerve); peptides; diagram of experimental model (p. 1712); pain;.

P4304 Debeir,T., Saragovi,H.U., Cuello,A.C. A nerve growth factor mimetic TrkA antagonist causes withdrawal of cortical cholinergic boutons in the adult rat. Proc.Natl.Acad.Sci.USA 1999/4; 96(--):4067-4072. >>> Peptide C(92-96); Antibody, anti-NGF monoclonal; PBS; Dye, methylene blue; CSF/CNS (cortex); rat; 2004; 2 weeks; peptides; C(92-96) is a small peptide that blocks NGF-TrkA interactions.

P4235 Burne,M.J., Osicka,T.M., Comper,W.D. Fractional clearance of high molecular weight proteins in conscious rats using a continuous infusion method. Kidney Int. 1999; 55(--):261-270. >>> Glucose oxidase; Albumin, bovine serum; Immunoglobulin G; Transferrin; Lactate dehydrogenase; Lactoperoxidase; 3H tracer; Thimerosal; Radio-isotopes; SC; rat; 2001; 7 days; pumps were incubated for four hours in PBS w/2% azide, and were then wiped w/70% isopropanol before implantation; peptides.

P4005 Hawkins,R.L., Murata,T., Inoue,M., Mori,M., Torii,K. Activin antiserum infused into the lateral hypothalamic area affects operant behavior of rats fed lysine-deficient diet. P.S.E.B.M. 1998; 219(--):149-153. >>> Antibody, anti-activin IgY; CSF, artificial;; CSF/CNS (hypothalamus);; rat; 2001; 1-4 weeks; controls received mp w/vehicle; pumps replaced after 1 week; one week recovery after implantation of guide cannulas; cannula placement verified by histology.

P4004 Garin,E.H., Laflam,P., Chandler,L. Anti-interleukin 8 antibody abolishes effects of lipoid nephrosis cytokine. Pediatric Nephrology 1998; 12(--):381-385. >>> Cell culture supernatant factor; Antibody, anti-human interleukin 8; PBS, sterile; Albumin, bovine serum; IA (renal); rat; 2ML1; 5 days; controls received mp w/vehicle; functionality of mp verified by residual volume; heat-stretched PE-10 tubing used; immunology.

P4003 Huang,B.S., Veerasingham,S.J., Leenen,F.H.H. Brain "ouabain," ANG II, and sympathoexcitation by chronic central sodium loading in rats. Am.J.Physiol.(Heart Circ.Physiol.43) 1998; 274(-43-):H1269-H1276. >>> Antibody, fab fragments; gamma globulin; losartan; CSF, artificial;; CSF/CNS;; rat; 2ML2; 14 days; no comment posted.

P4002 Burr,A.W., Toole,K., Chapman,C., Hines,J.E., Burt,A.D. Anti-hepatocyte growth factor antibody inhibits hepatocyte proliferation during liver regeneration. J.Pathology 1998; 185(--):298-302. >>> Antibody, anti-hepatocyte growth factor monoclonal; PBS; IP; rat; 2001; 7 days; controls received mp w/IgG antibody; functionality of mp verified by antibody serum levels by ELISA; immunology.

P4001 Schmidt,J.T., Schachner,M. Role for cell adhesion and glycosyl (HNK-1 and oligomannoside) recognition in the sharpening of the regenerating retinotectal projection in goldfish. J.Neurobiol. 1998; 37(--):659-671. >>> Antibody, anti-HNK-1; Antibody, C183 monoclonal; Oligomannoside glycopeptide; Ringer's solution; CSF/CNS (tectal ventricle); fish (goldfish); 2002; 1003D; 7-21 days; controls received mp w/vehicle; peptides; external pump.

P3999 Kojima,Y., Tojo,M., Goldmann,D.A., Tosteson,T.D., Pier,G.B. Antibody to the capsular polysaccharide/adhesin protects rabbits against catheter-related bacteremia due to coagulase-negative staphylococci. J.Infect.Dis. 1990; 162(--):435-441. >>> Bacteria, staphylococcus epidermis; Antibody, anti-polysaccharide/adhesin; Heparin; Serum, rabbit; IV (jugular); rabbit; 2ML1; 7 days; induction of bacteremia with infected i.v. catheters; immunology; peptides; some animals received 2 catheter-pump combinations concomitantly - 1 w/heparin infused through infected catheter tubing, and the second w/antibody or rabbit serum infused via sterile catheter tubing.

P3998 Lucidi-Phillipi,C.A., Clary,D.O., Reichardt,L.F., Gage,F.H. TrkA activation is sufficient to rescue axotomized cholinergic neurons. Neuron 1996; 16(--):653-663. >>> Antibody, anti-rat tyrosine receptor kinase A; Antibody, anti-NGF receptor immunoreactive p75; Nerve growth factor; Raf tyrosine receptor kinase A; CSF, artificial; Serum, rabbit; CSF/CNS;; rat; 2 weeks; cannula placement verified; peptides; polyclonal rabbit antibodies used; human beta NGF used; controls received fimbria fornix lesion alone or mp w/aCSF, REX, or rabbit serum.

P3986 Henke,P.K., Bergamini,T.M., Watson,A.L., Brittian,K.R., Powell,D.W., Peyton,J.C. Bacterial products primarily mediate fibroblast inhibition in biomaterial infection. J.Surg.Res. 1998; 74(--):17-22. >>> Antibody, anti-interferon gamma; Antibody, indomethacin; Antibody, anti-TNFa; Antibody, interleukin 1 alpha; PBS, sterile; ETOH; Indomethacin; SC; mice; 1007D; 7 days; immunology; pump implanted next to Dacron graft; peptides.

P3926 Nitta,A., Ogihara,Y., Onishi,J., Hasegawa,T., Furukawa,S., Nabeshima,T. Oral administration of propentofylline, a stimulator of nerve growth factor (NGF) synthesis, recovers cholinergic neuronal dysfunction induced by the infusion of anti-NGF antibody into the rat septum. Behav.Brain Research 1997; 83(--):201-204. >>> Antibody, anti-nerve growth factor, monoclonal; Saline; CSF/CNS (septum); rat; 16 days; controls received mp w/ control Ab.

P3919 Roberson,P.L., Dudek,S., Buchsbaum,D.J. Dosimetric comparison of bolus and continuous injections of CC49 monoclonal antibody in a colon cancer xenograft model. Cancer 1997; 80(--):2567-2575. >>> Antibody, monoclonal, CC49; PBS; 131I tracer; Albumin, human serum; IP; mice (nude); 2001; 7 days; comparison of IP bolus injections vs. mp; cancer; immunology.

P3918 Henke,P.K., Bergamini,T.M., Brittian,K.R., Polk Jr,H.C. Prostaglandin E2 modulates monocyte MHC-II (Ia) suppression in biomaterial infection. J.Surg.Res. 1997; 69(--):372-378. >>> Indomethacin; Antibody, anti-IL-1a; Antibody, anti-TNFa; PBS; ETOH; SC; mice; 7 days; controls received mp w/ vehicle; immunology.

P3891 Nair,R.S., Prieskorn,D.M., Miller,J.M., Mori,A., Gray,J., Carey,T.E. In vivo binding and hearing loss after intracochlear infusion of KHRI-3 antibody. Hear.Res. 1997; 107(--):93-101. >>> Antibody, KHRI-3 monoclonal; Immunoglobulin, IgG1; PBS, sterile; ear (cochlea); guinea-pig; 2002; 13 days; controls received mp w/IgG1; tissue perfusion (scala tympani); no stress (see pg. 99); stress/adverse reaction in control animals at cannula site (pg. 96); immunology; KHRI-3 antibody binds to supporting cells of the organ of corti in the inner ear.

P3678 Krenz,N.R., Meakin,S.O., Krassioukov,A.V., Weaver,L.C. Neutralizing intraspinal nerve growth factor blocks autonomic dysreflexia caused by spinal cord injury. J Neurosci 2003; 19(-17-):7405-7414. >>> Antibody, anti-nerve growth factor; Antibody, Immunoglobulin G; CSF, artificial; CSF/CNS (intrathecal); knee (articular cavity); Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; spinal cord injury; PE-10 intrathecal catheter tubing was perforated along its length and tip was sealed; PE-100 catheter used for knee joint infusion.

P3649 Eddy,A.A., Warren,J.S. Expression and function of monocyte chemoattractant protein-1 in experimental nephrotic syndrome. Clin.Immunol.Immunopathol. 1996; 78(-2-):140-151. >>> Immunoglobulin, MCP-1-neutralizing; IP; rat; 7 days; comparison of iv injections vs. mp; immunology; peptides; agent is anti-monocyte chemoattractant protein-1-neutralizing IgG.

P3521 Huang,B.S., Leenen,F.H.H. Blockade of brain "ouabain" prevents sympathoexcitatory and pressor responses to high sodium in SHR. Am.J.Physiol.(Heart Circ.Physiol.40) 1996; 271(--):H103-H108. >>> Antibody, Fab fragments; Gamma globulin; Saline; CSF/CNS; rat; 2002; 4 weeks; pumps replaced after 7 weeks.

P3511 Nitta,A., Ogihara,Y., Onishi,J., Hasegawa,T., Furukawa,S., Nabeshima,T. Propentofylline prevents neuronal dysfunction induced by infusion of anti-nerve growth factor antibody into the rat septum. Eur.J.Pharmacol. 1996; 307(--):1-6. >>> Antibody, anti-nerve growth factor, monoclonal; Saline; CSF/CNS (septum); rat; 16 days; new animal model for dementia.

P3414 Teramoto,T., Onda,M., Tokunaga,A., Asano,G. Inhibitory effect of anti-epidermal growth factor receptor antibody on a human gastric cancer. Cancer 1996; 77(--):1639-1645. >>> Antibody, anti-EGF receptor monoclonal; PBS; SC; mice (nude); 2002; 14 days; controls received mp w/PBS; comparison of IP injections vs. mp; cancer.

P3407 Leonard,J.P., Neben,T.Y., Kozitza,M.K., Quinto,C.M., Goldman,S.J. Constant subcutaneous infusion of rhIL-11 in mice: efficient delivery enhances biological activity. Exp.Hematol. 1996; 24(--):270-276. >>> Interleukin-11; Antibody, anti-interleukin-1 receptor; Serum, mouse; Saline, sterile; SC; mice; 3, 7, 10, 13 days; controls received mp w/vehicle; comparison of sc injections vs. mp; immunology; peptides; cardiovascular; "Compared to SC injection, both the magnitude and duration of the platelet increase were significantly enhanced following continuous SC infusion." (pg. 270).

P3404 Yoshimura,N., Lee,C.J., Shiho,O., Kita,M., Imanishi,J., Oka,T. Local immunosuppressive therapy with monoclonal anti-T-cell antibody on renal allograft survival in the rat. II. Phenotypic and functional assessment of spleen cells. Transplant.Proc. 1995; 27(-1-):390-391. >>> Antibody, monoclonal, OX-19; Saline; IA (renal); IV (femoral); rat; 7 days; immunology; arterial infusion proved more effective than venous infusion.

P3275 McGowan,M.K., Andrews,K.M., Grossman,S.P. Chronic intrahypothalamic infusions of insulin or insulin antibodies alter body weight and food intake in the rat. Physiol.Behav. 1992; 51(--):753-766. >>> Insulin, porcine; Antibody, specific insulin; Saline, sterile; CSF/CNS (ventromedial hypothalamus); CSF/CNS (third ventricle); rat; 2002; no duration posted; controls received mp w/saline or c-peptide antibodies; peptides; pump reservoir siliconized to prevent adsorption; 7 day saline infusion allowed surgical recovery.

P3101 Wen,T.-C., Yoshimura,H., Matsuda,S., Aburaya,J., Kushihata,F., Sakanaka,M. Protective effect of basic fibroblast growth factor-heparin and neurotoxic effect of platelet factor 4 on ischemic neuronal loss and learning disability in gerbils. Neuroscience 1995; 65(-2-):513-521. >>> Platelet factor 4; Fibroblast growth factor; Antibody, bfgf neutralizing; Immunoglobulin, normal rabbit; PBS; Albumin, bovine serum; Heparin; CSF/CNS; gerbil; 2,7 days; controls received mp with saline; peptides; recomb. basic FGF used; ischemia (cerebral).

P3094 Wiig,H., Kaysen,G.A., Al-Bander,H.A., De Carlo,M., Sibley,L., Renkin,E.M. Interstitial exclusion of IgG in rat tissues estimated by continuous infusion. Am.J.Physiol.(Heart Circ.Physiol.35) 1994; 35(--):H212-H219. >>> Immunoglobulin, rat; 125I tracer; Radio-isotopes; rat; 2001; 5,7 days; functionality of mp verified by plasma levels; no stress (see pg. H213); immunology.

P3087 Xu,B., Sahu,A., Crowley,W.R., Leranth,C., Horvath,T., Kalra,S.P. Role of neuropeptide-Y in episodic luteinizing hormone release in ovariectomized rats: an excitatory component and opioid involvement. Endocrinology 1993; 133(-2-):747-754. >>> Antibody, neuropeptide Y; Serum, normal rabbit; CSF, artificial;; CSF/CNS (third ventricle); rat; 2001D; no duration posted; controls received mp with vehicle.

P2857 Pizcueta,P., Luscinskas,F.W. Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo. Am.J.Pathol. 1994; 145(-2-):461-469. >>> Antibody, monoclonal, MEL-14; SC; mice; 1003D; no duration posted; controls received mp with saline; immunology.

P2838 Feurle,G.E., Hamscher,G., Firat,A.E. The role of CCK and its analogues in the organogenesis of the fetal rat pancreas. Pancreas 1995; 10(-3-):281-286. >>> MK-329; Immunoglobulin, antigastrin/CCK; DMSO; Tween 80; NaCl; Serum, rat; SC; rat; 2ML4; no duration posted; controls received mp with vehicles; immunoglobulin is monoclonal IgG.

P2805 Keirstead,H.S., Dyer,J.K., Sholomenko,G.N., McGraw,J., Delaney,K.R., Steeves,J.D. Axonal regeneration and physiological activity following transection and immunological disruption of myelin within the hatchling chick spinal cord. J.Neurosci. 1995; 15(-10-):6963-6974. >>> Serum, complement proteins, guinea pig; Antibody, lgG GalC polyclonal; Antibody, IgM 04 polyclonal; PBS; CSF/CNS (spinal cord, intrathecal); bird (chicken); 2ML2; 1007D; 5 or 12 or 14 days; controls received guinea pig serum only, antibodies only, or PBS only; immunology; antibodies are myelin/oligodendrocyte-specific, complement-binding; GalC is galactocerebroside antibody.

P2768 Nakata,N., Kato,H., Kogure,K. Inhibition of ischaemic tolerance in the gerbil hippocampus by quercetin and anti-heat shock protein-70 antibody. NeuroReport 1993; 4(--):695-698. >>> Quercetin; Antibody, anti-HSP70; DMSO; PBS; Albumin, bovine serum; CSF/CNS; gerbil; 4 or 7 days; controls received mp w/vehicles; quercetin, a flavonoid compound, inhibits the expression of HSP's.

P2756 Huang,B.S., Leenen,F.H.H. Brain 'ouabain,' sodium and arterial baroreflex in spontaneously hypertensive rats. Hypertension 1995; 25(-4 pt2-):814-817. >>> Antibody, Fab fragments; Gamma globulin, nonspecific; Saline; CSF/CNS; rat; 2002; 4 weeks; controls received low-sodium diet; pumps replaced after 2 weeks.

P2710 Dube,M.G., Xu,B., Crowley,W.R., Kalra,P.S., Kalra,S.P. Evidence that neuropeptide Y is a physiological signal for normal food intake. Brain Research 1994; 646(--):341-344. >>> Antibody, neuropeptide Y; Immunoglobulin, anti-neuropeptide Y; Serum, normal rabbit; CSF, artificial; Saline; Immunoglobulin, normal rabbit serum; CSF/CNS (third ventricle); rat; 2001D; 24 hours; controls received mp w/ rabbit serum; peptides.

P2597 Agro,A., Stanisz,A.M. Inhibition of murine intestinal inflammation by anti-substance P antibody. Reg.Immunol. 1993; 5(--):120-126. >>> Antibody, mouse anti-SP, polyclonal; Serum, normal rabbit; SC; mice; 2002; 14-17 days; controls received sham operations; immunology.

P2526 Alarid,E.T., Chen,P., Schaudies,R.P., Nicoll,C.S. Differential effects of an antiserum to epidermal growth factor on the development of transplanted rat embryos and fetal structures in vivo. Growth Factors 1993; 8(--):235-243. >>> Antibody, anti-epidermal growth factor; Epidermal growth factor; Water; Glycerol; Sodium nitrate; Heparin; Albumin; IA (renal); rat; 2002; 14 days; tissue perfusion (fetal tissue transplants); peptides.

P2511 Lee,C.J., Yoshimura,N., Shiho,O., Kita,M., Oka,T. Local immunosuppressive therapy with monoclonal anti-T cell antibody on renal allograft survival in the rat. Clin Exp Immunol 1993; 91(--):362-367. >>> Antibody, monoclonal; Saline; IA (renal); IV (femoral); rat; 2ML1; 7 days; tissue perfusion (kidney allograft); dose-response (table, p.364); immunology; comparison of single IV bolus, systemic IV infusion, or regional IA infusion.

P2367 Monno,A., Rizzi,M., Samanin,R., Vezzani,A. Anti-somatostatin antibody enhances the rate of hippocampal kindling in rats. Brain Research 1993; 602(-1993-):148-152. >>> Antibody, anti-somatostatin;; PBS; CSF/CNS (dorsal hippocampus); rat; 2002; 30 days; pumps replaced at day 14.

P2099 Alarid,E.T., Schlechter,N.L., Russell,S.M., Nicoll,C.S. Evidence suggesting that insulin-like growth factor-I is necessary for the trophic effect of insulin on cartilage growth in vivo. Endocrinology 1992; 139(-4-):2305-2309. >>> Antibody, anti-IGF I; Insulin; 125I tracer; Heparin; Radio-isotopes; Serum, rabbit; IA (iliac); rat; 2001; 7 days; functionality of mp verified by RIA; dose-response; peptides.

P2023 Takeda,Y., Miralles,F., Daher,N., Escribano,M.J. Radioimmunolocalization of the monoclonal antibody J28 in early transformation stages in N-nitrosobis(2-hydroxypropyl)amine-induced pancreatic tumors in the Syrian golden hamster. J.Cancer Res.Clin.Oncol. 1992; 118(--):377-385. >>> Antibody; 125I tracer; Radio-isotopes; IP; hamster; 1003D; no duration posted; cancer; peptides.

P2012 Takeda,S., Pier,G.B., Kojima,Y., Tojo,M., Muller,E., Tosteson,T., Goldmann,D.A. Protection against endocarditis due to staphylococcus epidermidis by immunization with capsular polysaccharide/adhesin. Circulation 1991; 84(-6-):2539-2546. >>> Antibody; Heparin; Antibody, monoclonal; IV (jugular); rabbit; 2ML1; 7 days; immunology; monoclonal antibody was IXB2; multiple pumps per animal (2).

P1890 Alarid,E.T., Cunha,G.R., Young,P., Nicoll,C.S. Evidence for an organ- and sex-specific role of basic fibroblast growth factor in the development of the fetal mammalian reproductive tract. Endocrinology 1991; 129(-4-):2148-2154. >>> Antibody, anti-fibroblast growth factor; Glycerol; Sodium azide; IA (suprarenal); rat; 2002; 14 days; tissue perfusion (graft); peptides.

P1876 Gage,F.H., Batchelor,P., Chen,K.S., Chin,D., Higgins,G.A., Koh,S., Deputy,S., Rosenberg,M.B., Fischer,W., Bjorklund,A. NGF receptor reexpression and NGF-mediated cholinergic neuronal hypertrophy in the damaged adult neostriatum. Neuron 1989; 2(--):1177-1184. >>> Antibody, anti-nerve growth factor; Nerve growth factor; PBS; Serum, rat; CSF/CNS (striatum); rat; 2002; 2 weeks; peptides.

P1818 Poltorak,M., Logan,S., Freed,W.J. Intraventricular xenografts: chronic injection of antibodies into the CSF provokes granulomatosis reactions but Ia antibodies do not enhance graft survival. Reg.Immunol. 1989; 2(-4-):197-202. >>> Antibody; Antibody, monoclonal; PBS; CSF/CNS; rat; 2002; no duration posted; three antibodies were: rat anti-mouse IgG, mouse anti-rat Ia MAB, mouse anti-penicillin MAB.

P1777 Schmidt,J.T., Shashoua,V.E. Antibodies to ependymin block the sharpening of the regenerating retinotectal projection in goldfish. Brain Research 1988; 446(--):269-284. >>> Antibody, antiependymin IgG; CSF/CNS; fish; 2002; 14-20 days; functionality of mp verified by measuring residual volume; schematic p. 271; external pump.

P1690 Liu,L., Russell,S.M., Nicoll,C.S. Analysis of the role of basic fibroblast growth factor in growth and differentiation of transplanted fetal rat paws and intestines. Endocrinology 1990; 126(-3-):1764-1770. >>> Antibody, anti-fibroblast growth factor; Fibroblast growth factor; Glycerol; Heparin; Saline; Sodium azide; Sodium citrate; IA (renal); rat; 2001; 2002; 6-11 days; tissue perfusion: tissue graft; good methods for FGF/pg. 1765; peptides; basic FGF used.

P1617 Connor,D., Fone,K.C.F., Bennett,G.W. Behavioural and biochemical effects of chronic intrathecal infusion of TRH antibodies. Br.J.Pharmacol. 1989; 97(--):571P-. >>> Antibody, anti-thyrotropin releasing hormone; Saline; Serum; CSF/CNS (spinal cord, intrathecal); rat; 14 days; peptides.

P1530 Arnauld,E., Bibene,V., Meynard,J., Rodriguez,F., Vincent,J.-D. Effects of chronic ICV infusion of vasopressin on sleep-waking cycle of rats. Am.J.Physiol. 1989; 256(--):R674-R684. >>> Antibody, anti-oxytocin; Antibody, anti-vasopressin, arginine; Oxytocin, 2-phenylalanine,8-ornithine; Serum, rabbit; Oxytocin; Vasopressin, 1-desamino-8-D arginine; Vasopressin, arginine; CSF, artificial; CSF/CNS; rat; 2002; 9, 10 days; dose-response; functionality of mp verified by plasma levels; peptides.

P1442 Manin,M., Balage,M., Larue-Achagiotis,C., Grizard,J. Chronic intracerebroventricular infusion of insulin failed to alter brain insulin-binding sites, food intake, and body weight. J.Neurochem. 1988; 51(--):1689-1695. >>> Insulin, anti-porcine; Antibody; Insulin, porcine; Glutamic acid; Saline; CSF/CNS; rat; 2002; 1, 2, 3, 5, 6, 9, 10 days; dose-response; delivery rate verified; functionality of mp verified by plasma levels; stability; peptides.

P1384 Liu,L., Nicoll,C.S. Evidence for a role of basic fibroblast growth factor in rat embryonic growth and differentiation. Endocrinology 1988; 123(-4-):2027-2031. >>> Antibody, fibroblast growth factor; Serum, rabbit; Fibroblast growth factor; Glycerol; Heparin, porcine; Sodium azide; Sodium citrate; Water; IA (renal); rat; 2001; 2002; 6, 10 days; antibody, transplanted embryo in kidney was perfused using renal artery; peptides; tissue perfusion; basic FGF used.

P1149 Wood,J.M., Baum,H.-P., Bews,J.P.A., Wachsmuth,E.D., Heusser,C., Hofbauer,K.G. Effects of chronic administration of a monoclonal antibody against human renin in the marmoset. Clin.and Exper.-Theory and Practice 1987; A9(-8/9-):1467-1478. >>> Antibody, monoclonal, R-33616; Saline; IP; monkey; 2002; 14 days; controls received mp w/ saline; 2 doses of agent infused; R-33616 challange 7 wks. post infusion; immunology.

P1136 Pimm,M.V., Clegg,J.A., Baldwin,R.W. Biodistribution and tumour localization of radiolabelled monoclonal antibody during continuous infusion in nude mice with human tumour xenografts. Eur.J.Cancer Clin.Oncol. 1987; 23(-5-):521-527. >>> Antibody, 791T/36; Immunoglobulin, IgG2b; Radio-isotopes; 125I tracer; 131I tracer; Saline; SC; mice (nude); 2002; 5, 15 days; dose-response; half-life; mp implanted contralaterally to xenografted tumors; concomitant and simult. infusion; 125I tracer used with IgG2b; comparison of sc, iv injections vs. mp infusion; functionality of mp verified by chromatography; stability; cancer.

P1029 Buonomo,F.C., Sabacky,M.J., Della-Fera,M.A., Baile,C.A. Effects of somatostatin immunoneutralization on growth and endocrine parameters in chickens. Domest.Anim.Endocrinol. 1987; 4(-3-):191-200. >>> Antibody, somatostatin; Saline; Serum, goat; SC; bird (chicken); 2002; 2 weeks; controls received mp w/ saline or goat serum; comparison of bolus inject. vs. IV inject. vs. mp infusion; immunology; peptides.

P0730 Keller,R. Repression of lymphatic metastasis by a second implant of the same tumor. Invasion Metastasis 1985; 5(--):295-308. >>> Antibody, ascitic fluid; Sodium citrate; SC; rat; 2002; no duration posted; stress/adverse reaction (within 6-12 days of implant, mps tightly surrounded by fibrous tissue which may have caused mp malfunction&necessitated them being replaced after 2 wks.); cancer.

P0473 Camp,R.C., Koestner,A., Vinores,S.A., Capen,C.C. The effect of nerve growth factor and antibodies to nerve growth factor on ethylnitrosourea-induced neoplastic proliferation in rat trigeminal nerves. Vet.Pathol. 1984; 21(-1-):67-73. >>> Antibody, anti-nerve growth factor; Nerve growth factor; Saline; SC; rat; 2001; 1 week; cancer therapy; biological activity of NGF verified after 1 week - stability; peptides.

P0046 Herlyn,D.M., Steplewski,Z., Herlyn,M.F., Koprowski,H. Inhibition of growth of colorectal carcinoma in nude mice by monoclonal antibody. Cancer Res. 1980; 40(-3-):717-721. >>> Antibody, P3 mouse myeloma; Antibody, ascitic fluid; Antibody, monoclonal carcinoma; IP; SC; mice (nude); 14 days; 1st pump ip for 7 days, replaced sc after 7 days; comparison of ip injection vs. infusion; immunotherapy; cancer.