References on the Administration of Agents to the Solid Tissue of the Brain Using ALZET® Osmotic Pumps

16337 Figge, D. A., Eskow Jaunarajs, K. L., Standaert, D. G. Dynamic DNA Methylation Regulates Levodopa-Induced Dyskinesia. J Neurosci 2016; 36(-24-):6514-24. >>> RG108; Cyclodextrin, hydroxypropyl-B-; CSF/CNS (dorsal striatum); Rat; 2004; Controls received mp w/ vehicle; animal info (male Sprague-dawley, 60– 90 days old, 180-200 g); functionality of mp verified by behavioral testing; 5% cyclodextrin used; Plastics One unilateral guide cannula used; post op. care (7 days of care, buprenorphine and wound care for pain management); behavioral testing (forepaw adjusting steps test); tissue perfusion (brain); delayed delivery “additional polyethylene tubing was added to provide a 14 d prime of vehicle before RG-108 administration” (pg. 6515); “rats were given a unilateral dopamine lesion to the left medial forebrain bundle” (pg. 6515); Brain coordinates: anteroposterior, 0 from bregma, -3mmlateral from midline, and -3.5 mm from the dura; Dose (100 uM);.

16188 Tamagawa, T., Shinoda, M., Honda, K., Furukawa, A., Kaji, K., Nagashima, H., Akasaka, R., Chen, J., Sessle, B. J., Yonehara, Y., Iwata, K. Involvement of Microglial P2Y12 Signaling in Tongue Cancer Pain. J Dent Res 2016; 95(-10-):1176-82. >>> MRS2395; DMSO; PBS; CSF/CNS (cisterna magna); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Fischer, 100-200g); 25% DMSO used; cancer (tongue squamous cell carcinoma SCC-158, JCRB0231; JCRB); behavioral testing (head-withdrawal); tissue perfusion (cisterna magna); used PE tubing 0.8mm diameter; MRS2395 is an P2Y12R antagonist;.

16119 Favero, M., Cangiano, A., Busetto, G. The timing of activity is a regulatory signal during development of neural connections. J Mol Neurosci 2014; 53(-3-):324-9. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); rat; animal info: rat strain AO; functionality of mp verified by establishment of a chronic conduction block; tissue perfusion (sciatic nerve);.

16094 Barth, R. F., Yang, W., Nakkula, R. J., Byun, Y., Tjarks, W., Wu, L. C., Binns, P. J., Riley, K. J. Evaluation of TK1 targeting carboranyl thymidine analogs as potential delivery agents for neutron capture therapy of brain tumors. Appl Radiat Isot 2015; 106(--):251-5. >>> N5-2OH; DMSO; CSF/CNS (intracerebral); IV; rat; 2001D; Controls received mp w/ vehicle, untreated controls; %35 of DMSO; cancer (brain tumor; F98 glioma model); tissue perfusion (brain tissue); brain tissue distribution; Animals were irradiated for 5 min at the MITR-II reactor;“intracerebral. administration of BPA did not increase the tumor boron uptake compared to that obtained following i.v. administration” (pg. 252); Dose: 500 ug.

16075 Yi, Q. Y., Qi, J., Yu, X. J., Li, H. B., Zhang, Y., Su, Q., Shi, T., Zhang, D. M., Guo, J., Feng, Z. P., Wang, M. L., Zhu, G. Q., Liu, J. J., Shi, X. L., Kang, Y. M. Paraventricular Nucleus Infusion of Epigallocatechin-3-O-Gallate Improves Renovascular Hypertension. Cardiovasc Toxicol 2016; 16(-3-):276-85. >>> gallate, Epigallocatechin-3-O; CSF, artificial; CSF/CNS (paraventricular nucleus); Rat; 1004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-275g); bilateral cannula used; post op. care (buprenorphine 0.01 mg/kg SC 12 hours post); tissue perfusion (paraventricular nucleus); tissue perfusion (paraventricular nucleus); tissue perfusion (paraventricular nucleus); Dose (20 ug/h); Brain coordinates“1.8 mm posterior, 0.4 mm lateral to the bregma and 7.9 mm ventral to the zero level“ pg 277;.

16068 Wu, K. L., Wu, C. W., Chao, Y. M., Hung, C. Y., Chan, J. Y. Impaired Nrf2 regulation of mitochondrial biogenesis in rostral ventrolateral medulla on hypertension induced by systemic inflammation. Free Radic Biol Med 2016; 97(--):58-74. >>> Endotoxin, LPS; coenzyme Q10; temple; Interleukin-1, receptor antagonist; butylhydroquinone, tert-; Saline; CSF, artificial; IP; ;CSF/CNS (cisterna magna); Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 8 weeks old, 250-280g); ALZET brain infusion kit 2 used; post op. care (IM procaine Penicillin 1000 IU); tissue perfusion (cisterna magna); cardiovascular; bp measured using tail cuff; Dose (LPS 1.2 mg/kg/day; coenzyme Q10 3.25 ug/ul/hr; tempol 5 ug/ul/hr; IL-1Ra 0.5 ug/ul/h);.

16014 Jackson, K. L., Palma-Rigo, K., Nguyen-Huu, T. P., Davern, P. J., Head, G. A. Actions of rilmenidine on neurogenic hypertension in BPH/2J genetically hypertensive mice. J Hypertens 2014; 32(-3-):575-86. >>> rilmenidine; Ringer’s solution; CSF/CNS (ICV); mice; 1002; Controls received mp w/ ringer’s solution vehicle; animal info (BPH/2J and BPN/3J mice); functionality of mp verified by behavioral tests; dose-response (pg 577-582, description and graphical representation); behavioral testing (pg 577; mice were exposed to aversive behavioral stimuli; restraint stress and dirty cage-switch tests conducted); tissue perfusion (brain tissue); antihypertensive; antihypertensive; Dose: 15ug/hr of rilmenidine.

15973 Yael Kusne,, Eugenio A. Carrera-Silva,, Anthony S. Perry,, Elisabeth J. Rushing,, Edward K. Mandell,, Justin D. Dietrich,, Andrea E. Errasti,, Daniel Gibbs,, Michael E. Berens,, Joseph C. Loftus,, Christopher Hulme,, Weiwei Yang,, Zhimin Lu,, Kenneth Aldape,, Nader Sanai,, Carla V. Rothlin,, Sourav Ghosh  Science Signaling 2015; 7(-338-):1-15. >>> PZ09; CSF/CNS (ICV); mice; animal info (Six- to 8-week-old female NOD-SCID, TNFa−/−, and control mice); dose-response (pg. 2-5); tissue perfusion (brain; gliobastomas); PZ09 aka small-molecule, benzimidazoleadenosine triphosphate–competitive aPKC inhibitor; xenografts; Dose: 10 uM PZ09.

15965 Liu, H., Li, Y., Wang, Y., Wang, X., An, X., Wang, S., Chen, L., Liu, G., Yang, Y. The distinct role of NR2B subunit in the enhancement of visual plasticity in adulthood. Mol Brain 2015; 8(--):49. >>> PPPA; TCN 201; Ro 25-6981; PBS; CSF/CNS (visual cortex); Mice; 1007D; 1002; Controls received mp w/ vehicle; animal info (C57BL6); tissue perfusion (visual cortex); cyanoacrylate adhesive; brain coordinates;.

15960 Li, H. B., Qin, D. N., Cheng, K., Su, Q., Miao, Y. W., Guo, J., Zhang, M., Zhu, G. Q., Kang, Y. M. Central blockade of salusin beta attenuates hypertension and hypothalamic inflammation in spontaneously hypertensive rats. Sci Rep 2015; 5(--):11162. >>> Immunoglobulin G, antisalusin b; CSF/CNS (paraventricular nucleus); Rat; 1004; Controls received mp w/ vehicle and control antibody; animal info (male, Wistar Kyoto, SHR, 10 weeks old); bilateral cannula used; post op. care (buprenorphine SC); tissue perfusion (paraventricular nucleus); cardiovascular; Cannula placement verified via histological analysis; bilateral infusion; bp measured using tail cuff; Dose (50, 100, 150 ng/kg/day);.

15937 Paine, T. A., Cooke, E. K., Lowes, D. C. Effects of chronic inhibition of GABA synthesis on attention and impulse control. Pharmacol Biochem Behav 2015; 135(--):97-104. >>> Allylglycine, L-; CSF, artificial; CSF/CNS (prefrontal cortex); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Sprague Dalwey); bilateral cannula used; Multiple pumps per animal (2); behavioral testing (standard time reaction test; locomotor activity); tissue perfusion (prefrontal cortex); enzyme inhibitor (glutamic acid decarboxylase); Dose (3.5 ug/0.5ul/h);.

15916 Kim, D. H., Moon, E. Y., Yi, J. H., Lee, H. E., Park, S. J., Ryu, Y. K., Kim, H. C., Lee, S., Ryu, J. H. Peptide fragment of thymosin beta4 increases hippocampal neurogenesis and facilitates spatial memory. Neuroscience 2015; 310(--):51-62. >>> Ac-SDKP; PBS; CSF/CNS (third ventricle); Mice; 1007D; animal info (male, C57BL6J, 25-28g, 8 weeks old); behavioral testing (Morris water maze); tissue perfusion (third ventricle); peptides; Cannula placement verified via histologic analysis; used Plastics one cannula; brain coordinates;.

15837 Renjun Wang, PhD; Qian Huang, MD; Rui Zhou, BSc; Zengxiang Dong, PhD;, Yunfeng Qi, PhD; Hua Li, MS; Xiaowei Wei, MS; Hui Wu, MS; Huiping Wang, PhD;, Christopher S. Wilcox, MD, PhD; Michael Hultström, MD, PhD; Xiaofu Zhou, PhD;, En Yin Lai, MD, PhD  Circ Heart Fail. 2016; 9(--):1-10. >>> AntagomiR-7b; RNA, small interfering GABBR1; angiotensin II; CSF/CNS (paraventricular nucleus); Rat; 1004; 1002; animal info (male, Wistar, 180-200g); pumps replaced after 4 weeks; bilateral cannula used; tissue perfusion (paraventricular nucleus); cardiovascular; peptides; bilateral infusion; Dose (AntagomiR-7b or Ad-siGABBR1 40 ng/h; antiotensin II 1 ng/kg/min);.

15832 Yasir A. Syed, Chao Zhao, Don Mahad, Wiebke Möbius, Friedrich Altmann, Franziska Foss, Aycan Sentürk, Amparo Acker‑Palmer, Gert Lubec, Kathryn Lilley, Robin J. M. Franklin, Klaus‑A. Nave, Mark R. N. Kotter  Acta Neuropathol. (Berl) 2016; 131(--):281-298. >>> Fc-IgG; ephrineB3, pre-clustered; PBS; CSF/CNS (caudal cerebellar peduncle); Rats; mice; Controls received mp w/ Fc-IgG only; animal info (Rats - female, Sprague Dawley; mice - EphrinB3 KO); neurodegenerative (multiple sclerosis); tissue perfusion (caudal cerebellar peduncle); Cannula placement verified via infusion of Evan's Blue;.

15799 Matthew McMillin,, Gabriel Frampton,, Matthew Quinn,, Samir Ashfaq,, Mario de los Santos, III,, Stephanie Grant,, Sharon DeMorrow  American Journal of Pathology 2016; 186(-2-):. >>> Vivo-morpholino; CSF/CNS (frontal cortex); Mice; Controls received mp w/ mismatched control morpholino; animal info (male, C57BL6, 25-30g); tissue perfusion (frontal cortex); tissue perfusion (frontal cortex);.

15235 Shinohara, K., Hata, T. Post-acquisition hippocampal NMDA receptor blockade sustains retention of spatial reference memory in Morris water maze. BEHAVIOURAL BRAIN RESEARCH 2014; 259(-;-):261-267. >>> AP5, D-; CSF, artificial; CSF/CNS (hippocampus); Rat; 1007D; Animal info (male, albino Wistar); pumps replaced every 7 days; Plastics One bilateral cannula used; Multiple pumps per animal (2); behavioral testing (morris water maze, probe test); tissue perfusion (hippocampus); Cannula placement verified via histological analysis; pumps primed for 24 hours in 37C saline; bilateral infusion;.

15220 Koneru, B., Bathina, C.S., Cherry, B.H., Mifflin, S.W. Mineralocorticoid receptor in the NTS stimulates saline intake during fourth ventricular infusions of aldosterone. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY 2014; 306(-1-):R61-R66. >>> Aldosterone; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; Animal info (Wistar-Kyoto, 275g); tissue perfusion (fourth ventricle); cardiovascular;.

15195 Benadiba, M., Costa, I.D., Santos, R.L.S.R., Serachi, F.O., Silva, D.D., Colquhoun, A., Colquhoun, A. Growth inhibitory effects of the Diruthenium-Ibuprofen compound, [Ru(2)Cl(Ibp)(4)], in human glioma cells in vitro and in the rat C6 orthotopic glioma in vivo1993. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 2014; 19(--):1025-1035. >>> Diruthenium-ibuprofen; Ethanol; CSF, artificial; CSF/CNS (intratumoral); Rat; 2002; Animal info (female, Wistar, 250-350g); ALZET brain infusion kit used; 15% ethanol used; comparison of injection vs mp; cancer (glioma); tissue perfusion (intratumoral, glioma);“Using the orthotopic C6 model the effects of either chronic 14-day treatment by intra-peritoneal injection of chronic 14-day intra-tumour infusion by an Alzet osmotic pump attached to a brain infusion cannula were tested. Tumour growth was reduced by both routes of administration with the osmoticpump appearing to be the less harmful route in terms of haematological responses.” pg 1033; Diruthenium-Ibuprofen aka RuIbp;.

15263 Zamykal, M., Martens, T., Matschke, J., Guenther, H.S., Kathagen, A., Schulte, A., Peters, R., Westphal, M., Lamszus, K., Lamszus, K. Inhibition of intracerebral glioblastoma growth by targeting the insulin-like growth factor 1 receptor involves different context-dependent mechanisms. NEURO-ONCOLOGY 2015; 17(--):1076-1085. >>> IMC-A12; Saline; CSF/CNS (intratumoral); Mice; 2004; Controls received mp w/ vehicle; animal info (Foxn1nu, 6-8 weeks old); cancer (glioblastoma); tissue perfusion (glioblastoma); IMC-A12 aka cixutumumab;.

15151 Yang, H.J., Kwon, D.Y., Kim, M.J., Kang, S., Moon, N.R., Daily, J.W., Park, S., Park, S. Red peppers with moderate and severe pungency prevent the memory deficit and hepatic insulin resistance in diabetic rats with Alzheimer's disease. Nutrition&Metabolism 2015; 12(--):U15-U26. >>> Amyloid protein, beta (25-35); CSF/CNS (hippocampus); Rat; Controls received mp w/ scrambled amyloid protein; animal info (male, Sprague Dawley, 192 +/-30g, ); neurodegenerative (Alzheimer's disease); behavioral testing (locomotive activity, passive avoidance, Morris water maze); tissue perfusion (CA1 subregion of hippocampus); diabetes;.

15089 Stark, R., Reichenbach, A., Lockie, S.H., Pracht, C., Wu, Q.L., Tups, A., Andrews, Z.B., Andrews, Z.B. Acyl Ghrelin Acts in the Brain to Control Liver Function and Peripheral Glucose Homeostasis in Male Mice. ENDOCRINOLOGY 2015; 156(--):858-868. >>> Ghrelin, acyl; CSF, artificial; CSF/CNS (third ventricle); Mice; 1007D; Controls received mp w/ vehicle; animal info (male, C57BL6); ALZET brain infusion kit 3 used; tissue perfusion (third ventricle);.

14928 Garofalo, S., D'Alessandro, G., Chece, G., Brau, F., Maggi, L., Rosa, A., Porzia, A., Mainiero, F., Esposito, V., Lauro, C., Benigni, G., Bernardini, G., Santoni, A., Limatola, C., Limatola, C. Enriched environment reduces glioma growth through immune and non-immune mechanisms in mice. Nature Communications 2015; 6(--):U26-U38. >>> Interleukin-15; brain-derived neurotrophic factor; PBS; CSF/CNS (striatum); Mice; 1007D; Controls received mp w/ vehicle; animal info (male, C57BL6, 3 weeks or 2 months old); ALZET brain infusion kit 3 used; cancer (glioma, U87MG human); tissue perfusion (right striatum); immunology; pumps primed in 37C saline overnight;.

14921 do Carmo, J.M., da Silva, A.A., Hall, J.E., do Carmo, J.M. Role of hindbrain melanocortin-4 receptor activity in controlling cardiovascular and metabolic functions in spontaneously hypertensive rats. JOURNAL OF HYPERTENSION 2015; 33(--):1201-1206. >>> SHU-9119; CSF/CNS (fourth ventricle); Rat; 2002; Animal info (male, Spontaneously hypertensive or Wistar-Kyoto, 275-325g, 15-17 weeks old); behavioral testing (food intake); tissue perfusion (fourth ventricle); cardiovascular; Cannula placement verified via Evan's Blue dye; bp measured using radiotelemetry (DSI);.

14864 Carrier, N., Saland, S.K., Duclot, F., He, H., Mercer, R., Kabbaj, M., Kabbaj, M. The Anxiolytic and Antidepressant-like Effects of Testosterone and Estrogen in Gonadectomized Male Rats. BIOLOGICAL PSYCHIATRY 2015; 78(--):259-269. >>> Fadrozole; Saline, sterile; CSF/CNS (dentate gyrus); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-270g, adult, orchidectomized); bilateral cannula used; behavioral testing (sucrose preference test, open field test); tissue perfusion (dentate gyrus); Cannula placement verified via a posteriori by sectioning on cryostat; bilateral infusion; coordinates; pumps primed for 48 hours in 37C sterile saline; used Plastics One cannula;.

14836 Barbier, E., Tapocik, J.D., Juergens, N., Pitcairn, C., Borich, A., Schank, J.R., Sun, H., Schuebel, K., Zhou, Z.F., Yuan, Q.P., Vendruscolo, L.F., Goldman, D., Heilig, M., Barbier, E. DNA Methylation in the Medial Prefrontal Cortex Regulates Alcohol-Induced Behavior and Plasticity. JOURNAL OF NEUROSCIENCE 2015; 35(--):6153-6164. >>> RG108; Cyclodextrin, 2-hydroxypropyl-B-; CSF/CNS; CSF/CNS (medial prefrontal cortex); Rat; 2002; 2004; Controls received mp w/ vehicle; animal info (male, Wistar, 200-225g); 5% cyclodextrin used; behavioral testing (two bottle free choice; alcohol self-administration); tissue perfusion (medial prefrontal cortex); dependence;.

14534 Zhou, Y.B., Sun, H.J., Chen, D., Liu, T.Y., Han, Y., Wang, J.J., Tang, C.S., Kang, Y.M., Zhu, G.Q., Zhu, G.Q. Intermedin in Paraventricular Nucleus Attenuates Sympathetic Activity and Blood Pressure via Nitric Oxide in Hypertensive Rats. Hypertension 2014; 63(--):330-+. >>> Intermedin; AM22-52; CSF, artificial; CSF/CNS (paraventricular nucleus); Rat; 1004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 160-180g); ALZET brain infusion kit used; tissue perfusion (paraventricular nucleus); antihypertensive; cardiovascular; Cannula placement verified via 2% Evans Blue dye injection; Intermedin aka IMD; AM22-52 is an adrenomedulllin receptor antagonist; bp measured using tail cuff;.

14526 Zhang, F., Sun, H.J., Xiong, X.Q., Chen, Q., Li, Y.H., Kang, Y.M., Wang, J.J., Gao, X.Y., Zhu, G.Q., Zhu, G.Q. Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats. ACTA PHYSIOLOGICA 2014; 212(--):17-27. >>> Apelin-13; CSF/CNS (paraventricular nucleus); Rat; 1002; Controls received mp w/ saline; animal info (male, Wistar Kyoto, 13 weeks old); tissue perfusion (paraventricular nucleus); cardiovascular;"Osmotic minipumps were used for continuously delivering chemicals at controlled rate for several weeks without the need for external connections, frequent handling or repeated dosing"pg 19; bp measured using tail cuff; hypertension;.

14518 Yuyama, K., Sun, H., Usuki, S., Sakai, S., Hanamatsu, H., Mioka, T., Kimura, N., Okada, M., Tahara, H., Furukawa, J., Fujitani, N., Shinohara, Y., Igarashi, Y., Yuyama, K. A potential function for neuronal exosomes: Sequestering intracerebral amyloid-beta peptide. FEBS Letters 2015; 589(--):84-88. >>> Exosome; PBS; CSF/CNS (hippocampus); Mice; Animal info (APP or C57BL6J, 13 months old); ALZET brain infusion kit used; neurodegenerative (Alzheimer's disease); tissue perfusion (hippocampus);.

14517 Yuyama, K., Sun, H., Sakai, S., Mitsutake, S., Okada, M., Tahara, H., Furukawa, J., Fujitani, N., Shinohara, Y., Igarashi, Y., Igarashi, Y. Decreased Amyloid-beta Pathologies by Intracerebral Loading of Glycosphingolipid-enriched Exosomes in Alzheimer Model Mice. Journal of Biological Chemistry 2014; 289(--):24488-24498. >>> Exosome solution, glycosphingolipid-enriched; PBS; CSF/CNS (hippocampus); Mice (transgenic); 1002; Controls received mp w/ vehicle; animal info (APP, 4 months old); ALZET brain infusion kit 3 used; neurodegenerative (Alzheimers); tissue perfusion (hippocampus);.

14485 Wu, Y.W., Tu, Q.S., Valverde, P., Zhang, J., Murray, D., Dong, L.Q., Cheng, J., Jiang, H., Rios, M., Morgan, E., Tang, Z.H., Chen, J.K., Chen, J.K. Central adiponectin administration reveals new regulatory mechanisms of bone metabolism in mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2014; 306(--):E1418-E1430. >>> Adiponectin, globular; adiponectin, full-length; CSF, artificial; CSF/CNS (third ventricle); Mice; Controls received mp w/ vehicle; animal info (male, APN-KO or WT, 20-25g, 4-6 weeks old); ALZET brain infusion kit 2 used; tissue perfusion (third ventricle); cyanoacrylate adhesive; Cannula placement verified via micrograph imaging; used tygon tubing to attach BIK cannula to pump;.

14482 Wu, K.L.H., Chao, Y.M., Tsay, S.J., Chen, C.H., Chan, S.H.H., Dovinova, I., Chan, J.Y.H., Chan, J.Y.H. Role of Nitric Oxide Synthase Uncoupling at Rostral Ventrolateral Medulla in Redox-Sensitive Hypertension Associated With Metabolic Syndrome. Hypertension 2014; 64(--):815-+. >>> Tempol; coenzyme Q10; CSF, artificial; CSF/CNS (cisterna magna); Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley 8 weeks old, 235-296g); post op. care (1000 IU IM); tissue perfusion (cisterna magna); cardiovascular; diabetes; used PE-10 catheter;.

14474 Wang, Y.K., Shen, D., Hao, Q., Yu, Q., Wu, Z.T., Deng, Y., Chen, Y.F., Yuan, W.J., Hu, Q.K., Su, D.F., Wang, W.Z., Wang, W.Z. Overexpression of angiotensin-converting enzyme 2 attenuates tonically active glutamatergic input to the rostral ventrolateral medulla in hypertensive rats. American Journal of Physiology-Heart and Circulatory Physiology 2014; 307(--):H182-H190. >>> A779; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 1007D; Controls received mp w/ vehicle; animal info (male, SHR and WKY); tissue perfusion (fourth ventricle);.

14429 Tan, M.S., Tan, L., Jiang, T., Zhu, X.C., Wang, H.F., Jia, C.D., Yu, J.T., Tan, L. Amyloid-beta induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer's disease. Cell Death&Disease 2014; 5(--):U228-U239. >>> RNA, small interfering NLRP1; RNA, small interfering, NLRP3; RNA, small interfering caspase-1; CSF, artificial; water, RNase-free; CSF/CNS (dorsal third ventricle); Mice (transgenic); Controls received mp w/ vehicle or control RNA; animal info (male, APP/PS1); functionality of mp verified by knowckdown of targetd gene; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); no stress"This dose of NLRP1 siRNA, caspase-1 siRNA or NLRP3 siRNA infusion was well tolerated, and no signs of neurotoxicity including hind-limb paralysis, vocalization, food intake, or neuroanatomical damage were observed in preliminary study."(see pg. 10); behavioral testing (morris water maze); tissue perfusion (dorsal third ventricle);"Our study using this approach of pump-mediated siRNA infusion is efficient in downregulation of NLRP1 mRNA (by about 60%) and protein (by about 50%) levels in APP/PS1 brain. And the treatment with control siRNA did not alter cerebral NLRP1 mRNA and protein levels compared with No siRNA-treated APP/PS1 mice, excluding an effect of pump-mediated infusion on NLRP1 expression levels."pg 8;.

14287 Malmevik, J., Rogers, M.L., Nilsson, M., Nakanishi, Y., Rush, R.A., Sirns, N.R., Muyderman, H., Muyderman, H. Selective transfection of microglia in the brain using an antibody-based non-viral vector. Brain Research 2014; 1586(--):12-22. >>> Scavenger receptor class B, type I; CSF/CNS (hippocampus); Rat; 1004; Animal info (male, Sprague Dawley, 250-350g); tissue perfusion (CA3 area of hippocampus); immunology; brain tissue distribution; Scavenger receptor class B, type I akd SR-BI;.

14257 Larson, T.A., Thatra, N.M., Lee, B.H., Brenowitz, E.A., Brenowitz, E.A. Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain. Journal of Neuroscience 2014; 34(--):13066-13076. >>> Z-VAD-FMK; Z-DEVD-FMK; Z-LEHD-FMK; DMSO; CSF/CNS (HVC); Bird (sparrow); 1007D; Controls received mp w/ vehicle; animal info (male, Gambel's white-crowned sparrows, adult); 1% DMSO used; behavioral testing (song behavior); tissue perfusion (control nucleus HVC); Cannula placement verified via histologic analysis;"We placed osmotic pumps into sealed microcentrifuge tubes filled with saline and mounted the tubes into custom-made"backpacks"that allowed the birds to fly freely."pg 13067; enzyme inhibitor (caspase-3, caspase-9, pan-caspase); bilateral infusion; used dental cement;.

14221 Hopp, S.C., Royer, S.E., D'Angelo, H.M., Kaercher, R.M., Fisher, D.A., Wenk, G.L., Wenk, G.L. Differential Neuroprotective and Anti-Inflammatory Effects of L-Type Voltage Dependent Calcium Channel and Ryanodine Receptor Antagonists in the Substantia Nigra and Locus Coeruleus. Journal of Neuroimmune Pharmacology 2015; 10(--):35-44. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; Animal info (male, F-344, 3 months old); behavioral testing (rotarod; open field test; forced swim test); tissue perfusion (fourth ventricle); immunology;.

14709 Desai, B.N., Harris, R.B.S., Harris, R.B.S. Leptin in the hindbrain facilitates phosphorylation of STAT3 in the hypothalamus. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2015; 308(--):E351-E361. >>> Leptin; CSF/CNS (third ventricle; fourth ventricle); Rat; 2002; Controls received mp w/ saline; animal info (male, Sprague Dawley, 275-300g); Multiple pumps per animal (2); tissue perfusion (third ventricle; fourth ventricle); bilateral infusion; one cannula to third ventricle second cannula to fourth ventricle; used plastics one cannula;.

14671 Bochner, D.N., Sapp, R.W., Adelson, J.D., Zhang, S.Y., Lee, H.M., Djurisic, M., Syken, J., Dan, Y., Shatz, C.J., Shatz, C.J. Blocking PirB up-regulates spines and functional synapses to unlock visual cortical plasticity and facilitate recovery from amblyopia. Science Translational Medicine 2014; 6(--):U13-U22. >>> Paired immunoglobulin-like receptor B, soluble; BSA; PBS; CSF/CNS (primary visual cortex); Mice; 1002; Controls received mp w/ vehicle; animal info (PirB -/-, P21); functionality of mp verified by staining for sPirB; tissue perfusion (primary visual cortex);.

14650 Adzovic, L., Lynn, A.E., D'Angelo, H.M., Crockett, A.M., Kaercher, R.M., Royer, S.E., Hopp, S.C., Wenk, G.L., Wenk, G.L. Insulin improves memory and reduces chronic neuroinflammation in the hippocampus of young but not aged brains. Journal of Neuroinflammation 2015; 12(--):U1-U10. >>> Insulin, recombinant cat; endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; Controls received mp w/ vehicle; animal info (male, F-344, 3 months old, 21 months old); bilateral cannula used; behavioral testing (morris water maze); tissue perfusion (fourth ventricle); bilateral infusion; used tygon tubing to attach cannula to pump;.

14636 Hiramatsu, R., Kawabata, S., Tanaka, H., Sakurai, Y., Suzuki, M., Ono, K., Miyatake, S.I., Kuroiwa, T., Hao, E., Vicente, M.G.H., Kawabata, S. Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin (TPFC): Application for Photodynamic Therapy and Boron Neutron Capture Therapy. Journal of Pharmaceutical Sciences 2015; 104(--):962-970. >>> TPFC; CSF/CNS; Rat; 2001D; Animal info (male, 200-250 g, F344 Fischer); ALZET brain infusion kit 2 used; TPFC, also known as Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin, is a carboranyl-containing chlorin of high boron content; cancer (glioma); tissue perfusion; convection-enhanced delivery.

13996 Wosiski-Kuhn, M., Erion, J.R., Gomez-Sanchez, E.P., Gomez-Sanchez, C.E., Stranahan, A.M., Stranahan, A.M. Glucocorticoid receptor activation impairs hippocampal plasticity by suppressing BDNF expression in obese mice. Psychoneuroendocrinology 2014; 42(--):165-177. >>> Corticosterone; Cyclodextrin, 2-hydroxypropyl-B;; CSF/CNS (hippocampus); Mice; Controls received mp w/ vehicle and aCSF; animal info (male, C57BL6J or db/db, 5 weeks old); functionality of mp verified by hippocampal corticosterone levels; Multiple pumps per animal (2); behavioral testing (y-maze apparatus); tissue perfusion (bilateral hippocampi); immunology; Cannula placement verified via histology; used Plastics One bilateral cannula; bilateral infusion;.

13961 Su, Q., Qin, D.N., Wang, F.X., Ren, J., Li, H.B., Zhang, M., Yang, Q., Miao, Y.W., Yu, X.J., Qi, J., Zhu, Z.M., Zhu, G.Q., Kang, Y.M., Qin, D.N. Inhibition of reactive oxygen species in hypothalamic paraventricular nucleus attenuates the renin-angiotensin system and proinflammatory cytokines in hypertension. TOXICOLOGY AND APPLIED PHARMACOLOGY 2014; 276(--):115-120. >>> Tempol; angiotensin II; Cerebrospinal fluid, artificial; saline, sterile; CSF/CNS (paraventricular nuclei); Rat; 1004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 250-275g); functionality of mp verified by increase bp; tissue perfusion (paraventricular nucleus); immunology;"The success rate of bilateral microinjection and vein infusion is respectively 65% and 78%."pg 116; bp measured using tail-cuff;.

13927 Sehgal, A., Chen, Q.M., Gibbings, D., Sah, D.W.Y., Bumcrot, D. Tissue-specific gene silencing monitored in circulating RNA. RNA-A PUBLICATION OF THE RNA SOCIETY 2014; 20(-2-):143-149. >>> RNA, small interfering; PBS; CSF/CNS (striatum); Rat; Animal info (male, Sprague Dawley); neurodegenerative (Parkinson's disease); tissue perfusion (striatum); gene therapy; used Plastics One 30g cannula; primed overnight in 37C saline;.

13856 Ma, J.H., Choi, B.R., Chung, C., Min, S.S., Jeon, W.K., Han, J.S., Han, J.S. Chronic brain inflammation causes a reduction in GluN2A and GluN2B subunits of NMDA receptors and an increase in the phosphorylation of mitogen-activated protein kinases in the hippocampus. MOLECULAR BRAIN 2014; 7(--):U1-U10. >>> Endotoxin, LPS; Cerebrospinal fluid, artificial; CSF/CNS (fourth ventricle); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Fischer-344); behavioral testing (morris water maze); tissue perfusion (fourth ventricle); immunology; LPS from E.coli, serotype 055:B5;.

13828 Kang, Y.M., Zhang, D.M., Yu, X.J., Yang, Q., Qi, J., Su, Q., Suo, Y.P., Yue, L.Y., Zhu, G.Q., Qin, D.N. Chronic infusion of enalaprilat into hypothalamic paraventricular nucleus attenuates angiotensin II-induced hypertension and cardiac hypertrophy by restoring neurotransmitters and cytokines. TOXICOLOGY AND APPLIED PHARMACOLOGY 2014; 274(-3-):436-444. >>> Angiotensin II; enalaprilat; Saline; cerebrospinal fluid, artificial; SC; CSF/CNS (paraventricular nucleus); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 275-300g); functionality of mp verified by increased bp; post op. care (buprenorphine); tissue perfusion (bilateral paraventricular nucleus); cardiovascular; peptides; antihypertensive; bp measured using tail-cuff; pumps primed in 37C saline; used wound clips; enalaprilat is an ACE inhibitor; Plastics One bilateral PVN cannulae; bilaterial infusion.

13821 Jiang, T., Yu, J.T., Zhu, X.C., Tan, M.S., Gu, L.Z., Zhang, Y.D., Tan, L., Tan, L. Triggering receptor expressed on myeloid cells 2 knockdown exacerbates aging-related neuroinflammation and cognitive deficiency in senescence-accelerated mouse prone 8 mice. NEUROBIOLOGY OF AGING 2014; 35(--):1243-1251. >>> RNA, small interfering TREM2; Cerebrospinal fluid, artifical; CSF/CNS (dorsal third ventricle); Mice; 1004; Controls received mp w/ vehicle or control siRNA; animal info (SAMP8, 6 months old); functionality of mp verified by decrease TREM2 protein levels pg. 1246; ALZET brain infusion kit used; neurodegenerative (Alzheimers); no stress (see pg. 1244); behavioral testing (Morris water maze); tissue perfusion (third ventricle); immunology;.

13810 Iijima, M., Yoshimizu, T., Shimazaki, T., Tokugawa, K., Fukumoto, K., Kurosu, S., Kuwada, T., Sekiguchi, Y., Chaki, S., Chaki, S. Antidepressant and anxiolytic profiles of newly synthesized arginine vasopressin V(1B) receptor antagonists: TASP0233278 and TASP0390325. British Journal of Pharmacology 2014; 171(--):3511-3525. >>> Allylglycine, 1-; CSF/CNS (hypothalamus); Rat; Animal info (male, Sprague Dawley, 211-246g); behavioral testing (forced swimming test, elevated plus maze, locomotor activity); tissue perfusion (dorsomedial hypothalamus); I-AG is a GAD inhibitor;.

13714 Bardou, I., Kaercher, R.M., Brothers, H.M., Hopp, S.C., Royer, S., Wenk, G.L. Age and duration of inflammatory environment differentially affect the neuroimmune response and catecholaminergic neurons in the midbrain and brainstem. NEUROBIOLOGY OF AGING 2014; 35(-5-):1065-1073. >>> Endotoxin, LPS; Cerebrospinal fluid, artificial; CSF/CNS (fourth ventricle); Rat; 2006; Controls received mp w/ vehicle; animal info (male, F-344 rats, 3 months or 9 months or 23 months old); post op. care (lidocane 1% on wound; 2ML isotonic saline SC injection; 2% tylenol in drinking water 3 days after surgery); tissue perfusion (fourth ventricle); long-term study; immunology; LPS aka lipopolysaccharide;.

13710 Avolio, E., Mahata, S.K., Mantuano, E., Mele, M., Alo, R., Facciolo, R.M., Talani, G., Canonaco, M., Avolio, E. ANTIHYPERTENSIVE AND NEUROPROTECTIVE EFFECTS OF CATESTATIN IN SPONTANEOUSLY HYPERTENSIVE RATS: INTERACTION WITH GABAERGIC TRANSMISSION IN AMYGDALA AND BRAINSTEM. Neuroscience 2014; 270(--):48-57. >>> Catestatin; muscimol; Saline; PBS; CSF/CNS (amygdala, central nucleus); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Spontaneously Hypertensive Rat, 3, 6, or 9 months old); ALZET brain infusion kit 2 used; tissue perfusion (central nucleus of amygdala); cardiovascular; antihypertensive; peptides; Cannula placement verified via histological analysis and methylene blue injection; Catestatin aka CST; muscimol aka MUS; used acrylic dental cement; bp measured using using radiotelemetry; CST is a catecholamine release inhibitor; muscimol is a GABAa receptor agonist;.

13651 Mohammad, M.G., Tsai, V.W.W., Ruitenberg, M.J., Hassanpour, M., Li, H., Hart, P.H., Breit, S.N., Sawchenko, P.E., Brownl, D.A. Immune cell trafficking from the brain maintains CNS immune tolerance. Journal of Clinical Investigation 2014; 124(-3-):1228-1241. >>> Fingolimod; DMSO; CSF/CNS (forebrain; hippocampus); SC (cervical lymph nodes); Mice (transgenic); Controls received mp w/ vehicle; animal info (female, C57BL6, 6-12 weeks old; 2D2 transgenic); ALZET brain infusion kit 3 used; 50% DMSO used; neurodegenerative (multiple sclerosis); tissue perfusion (forebrain, hippocampus, cervical lymph nodes); immunology; Cannula placement verified via Nissl staining;.

13638 Gmeiner, W.H., Lema-Tome, C., Gibo, D., Jennings-Gee, J., Milligan, C., Debinski, W. Selective anti-tumor activity of the novel fluoropyrimidine polymer F10 towards G48a orthotopic GBM tumors. Journal of Neuro-oncology 2014; 116(-3-):447-454. >>> F10; PBS; CSF/CNS (thalamus); Mice (nude); 1007D; Controls received mp w/ vehicle; animal info (nu/nu, 7 weeks old); ALZET brain infusion kit 3 used; cancer (human glioblastoma G48a); dose-response (pg.451-452, fig.3b and fig.4); no stress (see pg. 451); tissue perfusion (posterior thalamus); immunology;"F10 does not penetrate the BBB in healthy mice (data not shown), thus intra-cranial (i.c.) administration of F10 results in high local concentrations that may be therapeutically beneficial. Dose-finding studies in nude mice demonstrate that F10 administered i.c. using an Alzet osmotic mini-pump at doses up to 200 mg/kg administered over 7 days are well-tolerated and do not cause damage to normal brain (Fig. 2b)."pg 451; Pumps primed in 37C saline overnight; F10 is a novel antitumor agent;.

13637 Erion, J.R., Wosiski-Kuhn, M., Dey, A., Hao, S., Davis, C.L., Pollock, N.K., Stranahan, A.M. Obesity Elicits Interleukin 1-Mediated Deficits in Hippocampal Synaptic Plasticity. Journal of Neuroscience 2014; 34(-7-):2618-2631. >>> IL-1 receptor antagonist; Cerebrospinal fluid, artificial; CSF/CNS (hippocampus); Mice; Animal info (male, db/db C57BL6J); functionality of mp verified by ELISA of hippocampal IL1ra; behavioral testing (spatial recognition memory testing, novel-object preference, treadmill running); tissue perfusion (hippocampus); immunology; diabetes; Cannula placement verified via histology; Used Plastics One cannula.

13636 Cordeira, J.W., Felsted, J.A., Teillon, S., Daftary, S., Panessiti, M., Wirth, J., Sena-Esteves, M., Rios, M. Hypothalamic Dysfunction of the Thrombospondin Receptor alpha2beta-1 Underlies the Overeating and Obesity Triggered by Brain- Derived Neurotrophic Factor Deficiency. Journal of Neuroscience 2014; 34(-2-):554-565. >>> Gabapentin; Saline; CSF/CNS (ventromedial hypothalamus); Mice; 1004; Controls received mp w/ vehicle; animal info (male, BDNF 2L/2LCk-Cre, 8-10 weeks old); behavioral testing (feeding, locomotor activity); tissue perfusion (ventromedial hypothalamus); Cannula placement verified via post hoc histological analysis ;.

13512 Eberstaal, S., Sanden, E., Fritzell, S., Darabi, A., Visse, E., Siesjoe, P. Intratumoral COX-2 inhibition enhances GM-CSF immunotherapy against established mouse GL261 brain tumors. International Journal of Cancer 2014; 134(-11-):2748-2753. >>> Parecoxib; valdecoxib; IP; CSF/CNS (intratumoral); Mice; 1004; Animal info (C57BL/6, female, 8-10 wks old); cancer; tissue perfusion (intratumoral).

13485 Donegan, J.J., Girotti, M., Weinberg, M.S., Morilak, D.A. A Novel Role for Brain Interleukin-6: Facilitation of Cognitive Flexibility in Rat Orbitofrontal Cortex. Journal of Neuroscience 2014; 34(-3-):953-962. >>> Antibody, anti-IL-6; Saline; CSF/CNS (orbitofrontal cortex); Rat; 1004; Controls received mp w/ goat IgG; animal info (male, Sprague Dawley, adult, 220-240g); behavioral testing (learning); tissue perfusion (orbitofrontal cortex); immunology; Cannula placement verified via histology; Used dual-injection, osmotic pump compatible cannulae from Plastics One.

13480 Asavapanumas, N., Ratelade, J., Papadopoulos, M.C., Bennett, J.L., Levin, M.H., Verkman, A.S. Experimental mouse model of optic neuritis with inflammatory demyelination produced by passive transfer of neuromyelitis optica-immunoglobulin G. Journal of Neuroinflammation 2014; 11(-;-):U1-U11. >>> Antibody, rAb-53; complement, human; CSF/CNS (optic chiasm); Mice; 1003D; Controls received mp w/ non-NMO control IgG and complement; animal info (AQP4+/+ and AQP4-/-, 8-10 weeks old); tissue perfusion (optic chiasm); immunology; Cannula placement verified via Evan's blue dye; rAb-53 aka NMO-IgG;.

 

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What researchers are saying...

“Several approaches have been tested to improve the anti-tumor activity of cytotoxins and immunotoxins and to decrease toxicity and immunogenicity of these agents.”... “In our current study, we utilized a device for continuous infusion (ALZET pump) of drug as an alternate approach to increase the availability of drug. This approach resulted in improved anti-tumor effect and decreased toxic effects.” “Utilizing this device, IL-13 cytotoxin can be continuously administered systemically in body.” Kawakami et al., Molecular Medicine 2002;8(8):487-494.