References on the Administration of Peptides to the CNS Using ALZET^® Osmotic Pumps

Q0862 Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Serrano,R., Fernandez,A., Ros,M., Carrascosa,J.M., Arribas,C., Martinez,C., Andres,A., Gallardo,N. Regulation of Insulin-Stimulated Glucose Uptake in Rat White Adipose Tissue upon Chronic Central Leptin Infusion: Effects on Adiposity. Endocrinology 2011; 152(-4-):1366-1377. >>> Leptin, rat; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Wistar, 3-mo old); peptides.

Q0662 German,J.P., Thaler,J.P., Wisse,B.E., Oh,I., Sarruf,D.A., Matsen,M.E., Fischer,J.D., Taborsky,GJ Jr, Schwartz,M.W., Morton,G.J. Leptin Activates a Novel CNS Mechanism for Insulin-Independent Normalization of Severe Diabetic Hyperglycemia. Endocrinology 2011; 152(-2-):394-404. >>> Leptin; PBS; CSF/CNS; Rat; 11 days; Controls received mp w/ vehicle; animal info (adult, male, Wistar); peptides; pump implanted following 7-day recovery period after cannula insertion.

Q0674 Grobe,J.L., Buehrer,B.A., Hilzendeger,A.M., Liu,X.B., Davis,D.R., Xu,D., Sigmund,C.D. Angiotensinergic Signaling in the Brain Mediates Metabolic Effects of Deoxycorticosterone (DOCA)-Salt in C57 Mice. Hypertension 2011; 57(-3-):600-607. >>> Losartan; angiotensin II; CSF, artificial; CSF/CNS; SC; Mice; ; Controls received mp w/ vehicle; animal info (male, C57BL/6J, 6-8 wks old); peptides.

Q0905 Hansson,C., Haage,D., Taube,M., Egecioglu,E., Salome,N., Dickson,S.L. CENTRAL ADMINISTRATION OF GHRELIN ALTERS EMOTIONAL RESPONSES IN RATS: BEHAVIOURAL, ELECTROPHYSIOLOGICAL AND MOLECULAR EVIDENCE. Neuroscience 2011; 180(-;-):201-211. >>> Ghrelin; Saline; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 270-300 g); peptides; ALZET brain infusion kit 2 used.

Q0733 Murphy,K.J., Miller,A.M., Thelma,R., Cowley,F., Cox,F.F., Lynch,M.A. The age- and amyloid-beta-related increases in Nogo B contribute to microglial activation. NEUROCHEMISTRY INTERNATIONAL 2011; 58(-2-):161-168. >>> Amyloid protein, beta (1-40); amyloid protein, beta (1-42); CSF/CNS; Rat; 2004; 8 days; Controls received mp w/ reverse peptide 40-1; animal info (Wistar, male, 3 mo old, 250-350 g, 22 mo old, 550-650 g); peptides; ALZET brain infusion kit II used.

Q0788 Nakamura,Y., Fujita,Y., Ueno,M., Takai,T., Yamashita,T. Paired Immunoglobulin-like Receptor B Knockout Does Not Enhance Axonal Regeneration or Locomotor Recovery after Spinal Cord Injury. Journal of Biological Chemistry 2011; 286(-3-):1876-1883. >>> NEP1-40; DMSO; PBS; CSF/CNS (intrathecal); Mice; 2002; 14 days; Controls received mp w/ vehicle; animal info (C57BL/6J, 8 wks old, PIR-B-/-); NEP1-40 is a NgR antagonist peptide; 17% DMSO used; spinal cord injury.

Q0653 Syed,Y.A., Hand,E., Moebius,W., Zhao,C., Hofer,M., Nave,K.A., Kotter,M.R. Inhibition of CNS Remyelination by the Presence of Semaphorin 3A. Journal of Neuroscience 2011; 31(-10-):3719-3728. >>> Sema3A; PBS; CSF/CNS (caudal cerebellar peduncle); Rat; 2002; 10 days; Controls received mp w/ PBS; animal info (female, Sprague Dawley, 180-200 g); peptides; cyanoacrylate adhesive; Plastics One cannula used; pumps implanted after 10-day delay; tissue perfusion (caudal cerebellar peduncle).

Q0885 Tong,J., Mannea,E., Aime,P., Pfluger,P.T., Yi,C.X., Castaneda,T.R., Davis,H.W., Ren,X.Y., Pixley,S., Benoit,S., Julliard,K., Woods,S.C., Horvath,T.L., Sleeman,M.M., D'Alessio,D., Obici,S., Frank,R., Tschoep,M.H. Ghrelin Enhances Olfactory Sensitivity and Exploratory Sniffing in Rodents and Humans. Journal of Neuroscience 2011; 31(-15-):5841-5846. >>> Ghrelin; Saline, isotonic; CSF/CNS (third ventricle); Rat; 8 days; Controls received mp w/ vehicle; animal info (male, 300-350 g, Wistar, 280-350 g); peptides; behavioral testing (Sniff frequency, Conditioned odor aversion); cannula placement verified by angiotensin II dipsogenic thirst test.

Q0783 Zhai,H.Y., Heppner,F.L., Tsirka,S.E. Microglia/Macrophages Promote Glioma Progression. Glia 2011; 59(-3-):472-485. >>> Ganciclovir; macrophage/microglia inhibitory factor; tuftsin; CSF/CNS (intratumoral); Mice; 14, 28 days; Negative controls received mp w/ saline; animal info (12-16 wks old, male, CD11b-HSVTK +/-, 25-30 g, C57BL/6); cancer (glioma); Plastics One guide cannula used; macrophage/microglia inhibitory factor also known as MIF/TKP is a tripeptide; tuftsin also known as threonine-lysine-proline-arginin or TKPR.

Q0679 Zhu,D.L., Shi,J.P., Zhang,Y.D., Wang,B.R., Liu,W., Chen,Z.C., Tong,Q.A. Central Angiotensin II Stimulation Promotes beta Amyloid Production in Sprague Dawley Rats. PLoS One 2011; 6(-1-):U121-U129. >>> Angiotensin II; Saline, sterile; CSF/CNS; Rat; 2004; 1 week; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 280-310 g); peptides; ALZET brain infusion kit used.

Q0398 Alkadhi,K.A., Srivareerat,M., Tran,T.T. Intensification of long-term memory deficit by chronic stress and prevention by nicotine in a rat model of Alzheimer's disease. MOLECULAR AND CELLULAR NEUROSCIENCE 2010; 45(-3-):289-296. >>> Amyloid protein, beta (1-40); amyloid protein, beta 1-42; Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2 weeks; Controls received mp w/ amyloid protein beta non-pathogenic reverse peptide; animal info (adult, male, Wistar, 200-225 g); neurodegenerative (Alzheimer's disease).

Q0009 Cirillo,G., Cavaliere,C., Bianco,M.R., De Simone,A., Colangelo,A.M., Sellitti,S., Alberghina,L., Papa,M. Intrathecal NGF Administration Reduces Reactive Astrocytosis and Changes Neurotrophin Receptors Expression Pattern in a Rat Model of Neuropathic Pain. Cellular and Molecular Neurobiology 2010; 30(-1-):51-62. >>> Nerve growth factor, b-, recomb. rat; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (adult, male, Sprague-Dawley, 250-300 g); SCI; spinal cord injury; behavioral testing (thermal nociceptive testing, mechanical allodynia).

P9966 Fang,P.C., Barbay,S., Plautz,E.J., Hoover,E., Strittmatter,S.M., Nudo,R.J. Combination of NEP 1-40 Treatment and Motor Training Enhances Behavioral Recovery After a Focal Cortical Infarct in Rats. Stroke 2010; 41(-3-):544-549. >>> NEP (1-40); PBS; DMSO; CSF/CNS; Rat; 2ML4; 28 days; ALZET brain infusion kit 2 used; animal info (Long Evans, adult, male); 2.5% DMSO used; cannula implanted first, pump implanted following recovery period of 7 days; behavioral testing (skilled reach test, motor trainning, foot faul test, cylinder test); NEP (1-40) also known as NOGO extracellular peptide.

Q0146 Gao,Y.J., Xu,Z.Z., Liu,Y.C., Wen,Y.R., Decosterd,I., Ji,R.R. The c-Jun N-terminal kinase 1 (JNK1) in spinal astrocytes is required for the maintenance of bilateral mechanical allodynia under a persistent inflammatory pain condition. Pain 2010; 148(-2-):309-319. >>> D-JNK-1; SP 600125; Saline; DMSO; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 220-260 g., 20-30 g, JNK1 -/-, JNK2 -/-); PE-10 catheter used; 10% DMSO used; "We cannot exclude the possibility that the peptide inhibitor is degraded over time at the body temperature" pg 314.

P9943 Guesdon,B., Denis,R.G.P., Richard,D. Additive effects of olanzapine and melanin-concentrating hormone agonism on energy balance. Behavioural Brain Research 2010; 207(-1-):14-20. >>> Compound A; CSF/CNS (third ventricle); Rat; 2001; 2002; 13 days; Controls received mp w/ vehicle; saline pump replaced after 1 week; animal info (male, wistar, 250 g); Compound A is a mchr1 agonist solution, truncated; peptides.

Q0320 Huang,B.S., Ahmadi,S., Ahmad,M., White,R.A., Leenen,F.H.H. Central neuronal activation and pressor responses induced by circulating ANG II: role of the brain aldosterone-"ouabain" pathway. American Journal of Physiology-Heart and Circulatory Physiology 2010; 299(-2-):U207-U215. >>> Angiotensin II; FAD-286; aldosterone, d-; Saline; CSF, artificial; ethanol; SC; CSF/CNS; Rat; 2001D; 2001; 2002; 2004; 2 weeks; 1, 4 days; Controls received mp w/ vehicle; animal info (male, Wistar, 200-250 g); peptides; dose-response; enzyme inhibitor (aldosterone synthase); multiple pumps per animal (2); telemetry probe (model TA11PA-C40, DSI) used to measure BP and HR; 1% ethanol used with aldosterone.

Q0598 Ittner,L.M., Ke,Y.D., Delerue,F., Bi,M.A., Gladbach,A., van Eersel,J., Woelfing,H., Chieng,B.C., Christie,M.J., Napier,I.A., Eckert,A., Staufenbiel,M., Hardeman,E., Goetz,J. Dendritic Function of Tau Mediates Amyloid-beta Toxicity in Alzheimer's Disease Mouse Models. Cell 2010; 142(-3-):387-397. >>> TAT-NR2B9c; CSF, artificial; CSF/CNS; Mice; 1004; 56 days; Negative controls received mp w/ Tat-NR2BAA; animal info (6 wks old, APP23, WT); long-term study; pumps replaced after 28 days; ALZET brain infusion kit 3 (with one spacer) used; peptides; neurodegenerative (Alzheimer's Disease).

Q0898 Kasselman,L.J., Rutkove,S.B. APPLICATION OF ANGIOTENSIN II TO HEALTHY RAT SCIATIC NERVE CAN PRODUCE NEUROPATHY WITHOUT ASSOCIATED VASCULOPATHY. Muscle&Nerve 2010; 42(-6-):959-965. >>> Angiotensin II; CSF/CNS (sciatic nerve); Rat; 2004; 12 weeks; Controls received mp w/ PBS; animal info (male, Wistar, 250-300 g); peptides; long-term study; good methods, pg 960; no stress (see pg. 961) "All animals from both groups survived the surgery without complications"; pump connected to silicone tube that was connected to a piece of Gelfoam; Fig. 1, schematic of pump, tube, and gelfoam. pg 960.

Q0137 King,V.R., Hewazy,D., Alovskaya,A., Phillips,J.B., Brown,R.A., Priestley,J.V. THE NEUROPROTECTIVE EFFECTS OF FIBRONECTIN MATS AND FIBRONECTIN PEPTIDES FOLLOWING SPINAL CORD INJURY IN THE RAT. Neuroscience 2010; 168(-2-):523-530. >>> Fibronectin mat solution; peptide, fibronectin-derived synthetic; Saline; CSF/CNS (intrathecal); Rat; 1007D; 1, 3, 7 days; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, adult, laminectomized); silastic tubing used; spinal cord injury.

Q0128 Kinsey-Jones,J.S., Li,X.F., Knox,A.M.I., Lin,Y.S., Milligan,S.R., Lightman,S.L., O'Byrne,K.T. Corticotrophin-Releasing Factor Alters the Timing of Puberty in the Female Rat. Journal of Neuroendocrinology 2010; 22(-2-):102-109. >>> Corticotrophin-releasing factor; astressin-B; CSF, artificial; CSF/CNS; Rat; 2002; 14, 14 days; Controls received no treatment/surgery; peptides; no stress (see pg. 105); animal info (female, Sprague-Dawley, 28 days old); dose-response (fig. 1); neuroendocrinology.

P9890 Mehanna,A., Jakovcevski,I., Acar,A., Xiao,M.F., Loers,G., Rougon,G., Irintchev,A., Schachner,M. Polysialic Acid Glycomimetic Promotes Functional Recovery and Plasticity After Spinal Cord Injury in Mice. MOLECULAR THERAPY 2010; 18(-1-):34-43. >>> Polysialic acid, mimetic; human natural killer cell-1, mimetic, cyclic; PBS; CSF/CNS (subdural); Mice; 1002; 2 weeks; Control received mp w/ control peptide; peptides; no stress (see pg. 40); animal info (3-4 month old, female, C57BL/6J); spinal cord injury; catheter position confirmed by macrosopic examination; "no adverse effects of the pump implantation, such as infections, paralyses, or aberrant behavior, were observed." pg 40.

Q0246 Petervari,E., Garami,A., Soos,S., Szekely,M., Balasko,M. Age-dependence of alpha-MSH-induced anorexia. Neuropeptides 2010; 44(-4-):315-322. >>> Melanocyte stimulating hormone, alpha; Saline, pyrogen free; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; ALZET brain infusion kit used; animal info (male, Wistar, 6-9 wks old, 3-4, 6, 12, 18, 24 mo old).

Q0185 Sangiao-Alvarellos,S., Varela,L., Vazquez,M.J., Da Boit,K., Saha,A.K., Cordido,F., Dieguez,C., Lopez,M. Influence of Ghrelin and Growth Hormone Deficiency on AMP-Activated Protein Kinase and Hypothalamic Lipid Metabolism. Journal of Neuroendocrinology 2010; 22(-6-):543-556. >>> Ghrelin, acyl; Saline; CSF/CNS; Rat; 2001; 2ML2; 8 days; Controls received mp w/ vehicle; peptides; animal info (male, Lewis, wt, GH deficient (dwarf), 2-3 months old, 365 g, 222 g); cannula placement confirmed by methylene blue staining; endocrinology.

Q0252 Shinohara,M., Sato,N., Kurinami,H., Takeuchi,D., Takeda,S., Shimamura,M., Yamashita,T., Uchiyama,Y., Rakugi,H., Morishita,R. Reduction of Brain beta-Amyloid (A-beta) by Fluvastatin, a Hydroxymethylglutaryl-CoA Reductase Inhibitor, through Increase in Degradation of Amyloid Precursor Protein C-terminal Fragments (APP-CTFs) and A-beta Clearance. Journal of Biological Chemistry 2010; 285(-29-):22091-22102. >>> Leupeptin; E-64a; CSF, artificial; CSF/CNS; Mice (transgenic); 2002; 1 week; Controls received mp w/ vehicle; peptides; ALZET brain infusion kit 3 used; no stress (see pg 22092); neurodegenerative (Alzheimer's Disease); animal info (male, C57BL/6, APP23 Tg, 8 wks old); Lysosomal inhibitor; aCSF recipe.

Q0500 Tran,T.T., Srivareerat,M., Alkadhi,K.A. Chronic psychosocial stress triggers cognitive impairment in a novel at-risk model of Alzheimer's disease. NEUROBIOLOGY OF DISEASE 2010; 37(-3-):756-763. >>> Amyloid protein, beta (1-42); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2 weeks; Controls received mp w/ amyloid protein beta (42-1); peptides; animal info (Wistar, 200-225 g); 14-day pump used; neurodegenerative (Alzheimer's Disease).

Q0769 Tsiperson,V., Li,X.S., Schwartz,G.J., Raine,C.S., Shafit-Zagardo,B. GAS6 Enhances Repair Following Cuprizone-Induced Demyelination. PLoS One 2010; 5(-12-):U394-U402. >>> Growth arrest-specfic protein 6, recomb. human; PBS; CSF/CNS (corpus callosum); Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (C57Bl6J, male 8 wks old); peptides; cyanoacrylate adhesive; ALZET brain infusion kit 3 used.

Q0341 Verpelli,C., Bertani,G., Cea,V., Patti,M., Bikfalvi,A., Bello,L., Sala,C. Anti-Angiogenic Therapy Induces Integrin-Linked Kinase 1 Up-Regulation in a Mouse Model of Glioblastoma. PLoS One 2010; 5(-10-):U70-U81. >>> PF4-DLR; RNA, small interfering; PBS; SC; CSF/CNS (intrathecal); Mice (nude); 2004; 10, 20 days; Controls received mp w/ vehicle; animal info (6 wks old, nude); peptides; ILK1-siRNA.

Q0165 Abdul,H.M, Furman,J.L., Sama,M.A., Mathis,D.M., Norris,C.M  Mol Cel Pharmacol 2009; 2(-1-):7-14. >>> VIVT peptide; scrambled control peptide; CSF/CNS; Mice; 14 days; Controls received mp w/ scrambeled control peptide; peptides; animal info (14 months old, APP, P51); VIVT is an immunosuppressive peptide.

P9473 Anderson,G.M., Relf,H.L., Rizwan,M.Z., Evans,J.J. Central and Peripheral Effects of RFamide-Related Peptide-3 on Luteinizing Hormone and Prolactin Secretion in Rats. Endocrinology 2009; 150(-4-):1834-1840. >>> RF amide-related peptide-3; CSF, artificial; CSF/CNS; Rat; 1002; 2 weeks; ALZET brain infusion kit used; animal info (female, Sprague Dawley, 9-12 wks old, 250-300 g.); neuropeptide.

Q0394 Burgos-Ramos,E., Puebla-Jimenez,L., Hernandez-Pinto,A., rilla-Ferreiro,E. Sulfadiazine Partially Protects the Rat Temporal Cortex from Amyloid Beta Peptide (25-35)-Induced Alterations of the Somatostatinergic System. Neuroendocrinology 2009; 89(-4-):400-410. >>> Amyloid protein, beta (25-35); Acetic acid; CSF/CNS; Rat; 14 days; Controls received mp w/vehicle; animal info (male, Wistar, 200-250 g); peptides.

P9741 Burgos-Ramos,E., Puebla-Jimenez,L., rilla-Ferreiro,E. Minocycline prevents A-beta-(25-35)-induced reduction of somatostatin and neprilysin content in rat temporal cortex. LIFE SCIENCES 2009; 84(-7-8-):205-210. >>> Amyloid protein, beta (25-35); Acetic acid; CSF/CNS; Rat; 14 days; Controls received mp w/vehicle; peptides; animal info (male, Wistar, 200-250 g).

P9746 Catania,C., Sotiropoulos,I., Silva,R., Onofri,C., Breen,K.C., Sousa,N., Almeida,O.F.X. The amyloidogenic potential and behavioral correlates of stress. Molecular Psychiatry 2009; ;(-1-):95-105. >>> Amyloid protein, beta (1-40); Water, distilled; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/vehicle; functionality of mp verified; ALZET brain infusion kit used; peptides; animal info (male, Wistar, 14 months old); neurodegenerative (Alzheimer's disease); cannula patency verified.

P9561 Chan,S.H.H., Wu,K.L.H., Chang,A.Y.W., Tai,M.H., Chan,J.Y.H. Oxidative Impairment of Mitochondrial Electron Transport Chain Complexes in Rostral Ventrolateral Medulla Contributes to Neurogenic Hypertension. Hypertension 2009; 53(-2-):217-U230. >>> Angiotensin II; phenylephrine; Saline; CSF, artificial; IP; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/vehicle; peptides; post op. care (Penicillin); animal info (SHR, 5-6 wks old, 11-12 wks old, Wky, 5-6 wks old).

Q0438 Chan,S.H.H., Wu,C.A., Wu,K.L.H., Ho,Y.H., Chang,A.Y.W., Chan,J.Y.H. Transcriptional Upregulation of Mitochondrial Uncoupling Protein 2 Protects Against Oxidative Stress-Associated Neurogenic Hypertension. Circulation Research 2009; 105(-9-):886-U145. >>> Rosiglitazone; oligonucleotide, antisense; angiotensin II; CSF, artificial; DMSO; CSF/CNS; CSF/CNS (intrathecal, cisterna magna); 2001; 7 days; Controls received mp w/ vehicle; animal info (adult, male Sprague-Dawley, 10-11 wks old); antisense (UCP2); peptides; 0.5% DMSO used.

Q0432 Glick,M., Segal-Lieberman,G., Cohen,R., Kronfeld-Schor,N. Chronic MCH infusion causes a decrease in energy expenditure and body temperature, and an increase in serum IGF-1 levels in mice. Endocrine 2009; 36(-3-):479-485. >>> Melanin concentrating hormone; CSF, artificial; CSF/CNS; Mice; 2002; 14 days; Controls received mp w/ vehicle; peptides; cyanoacrylate adhesive; animal info (12 wks old, C57BL/6); cannula placement verified post morten with Evans Blue dye; endocrinology.

P9513 Harvey,P.A., Lee,D.H.S., Qian,F., Weinreb,P.H., Frank,E. Blockade of Nogo Receptor Ligands Promotes Functional Regeneration of Sensory Axons after Dorsal Root Crush. Journal of Neuroscience 2009; 29(-19-):6285-6295. >>> Nogo receptor, soluble fragment; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; stability verified by "separate tests" (up to 1 month, pg 6286); ALZET brain infusion kit 2 used; peptides; animal info (male, Sprague Dawley, 200-250 g.); sNGR.

P9640 Kang,Y.M., Ma,Y., Zheng,J.P., Elks,C., Sriramula,S., Yang,Z.M., Francis,J. Brain nuclear factor-kappa B activation contributes to neurohumoral excitation in angiotensin II-induced hypertension. Cardiovascular Research 2009; 82(-3-):503-512. >>> Angiotensin II; losartan; pyrrolidine dithiocarbamate; tempol; Saline; CSF, artificial; IV (femoral); CSF/CNS; Rat; 4 weeks; Controls received mp w/vehicle; peptides; multiple pumps per animal (2); animal info (adult, male, Sprague-Dawley, 275-300g); bilateral cannula used for multiple drug infusions.

P9705 Kimura,T., Nguyen,P.T.H., Ho,S.A., Tran,A.H., Ono,T., Nishijo,H. T-817MA, a neurotrophic agent, ameliorates the deficits in adult neurogenesis and spatial memory in rats infused i.c.v. with amyloid-beta peptide. British Journal of Pharmacology 2009; 157(-3-):451-463. >>> Amyloid protein, beta (1-40); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; 10 weeks; Controls received mp w/vehicle; long-term study; peptides; animal info (male, crj, Wistar, 7 wks old); behavioral testing (open field test).

Q0748 Lee,H.K., Seo,I.A., Suh,D.J., Park,H.T. Nidogen Plays a Role in the Regenerative Axon Growth of Adult Sensory Neurons Through Schwann Cells. Journal of Korean Medical Science 2009; 24(-4-):654-659. >>> Alkaline phosphatase tag; eTEM-AP, recomb.; CSF/CNS (sciatic nerve); Rat; 1002; 2 weeks; Controls received mp w/ alkaline phosphatase tag; animal info (adult, male, Sprague-Dawley, 150-300 g); peptides.

P9847 Moriya,R., Mashiko,S., Ishihara,A., Takahashi,T., Murai,T., Ito,J., Mitobe,Y., Oda,Z., Iwaasa,H., Takehiro,F., Kanatani,A. Comparison of independent and combined chronic anti-obese effects of NPY Y2 receptor agonist, PYY(3-36), and NPY Y5 receptor antagonist in diet-induced obese mice. Peptides 2009; 30(-7-):1318-1322. >>> Neuropeptide Y, D-Trp34; peptide YY (3-36); Saline; BSA; CSF/CNS; Mice; 2001; 2002; 7, 14 days; Controls received mp w/ vehicle; pumps replaced after 2 weeks; peptides; animal info (C57BL/6, 12-18 wks old); PBS/BSA in 2002 pump was replaced with NPP in 2001 after 2 week recovery period; obesity.

Q0701 Mouret,A., Lepousez,G., Gras,J., Gabellec,M.M., Lledo,P.M. Turnover of Newborn Olfactory Bulb Neurons Optimizes Olfaction. Journal of Neuroscience 2009; 29(-39-):12302-12314. >>> zVAD; Saline; DMSO; CSF/CNS (olfactory bulb); Mice; 1002; 15 days; Controls received mp w/ vehicle; animal info (2 mo old, male, C57BL/6J); peptides; functionality of mp verified by pump weight; ALZET brain infusion kit 3 used; 1.5% DMSO used; Chicago blue dye used to verify cannula placement.

P9624 Peier,A., Kosinski,J., Cox-York,K., Qian,Y., Desai,K., Feng,Y., Trivedi,P., Hastings,N., Marsh,D.J. The Antiobesity Effects of Centrally Administered Neuromedin U and Neuromedin S Are Mediated Predominantly by the Neuromedin U Receptor 2 (NMUR2). Endocrinology 2009; 150(-7-):3101-3109. >>> Neuromedin U, human; CSF, artificial; CSF/CNS; Mice; 14 days; Controls received mp w/vehicle; peptides; animal info (8 wks old, Nmur2 -/-).

Q0570 Petersen,P.S., Woldbye,D.P.D., Madsen,A.N., Egerod,K.L., Jin,C.Y., Lang,M., Rasmussen,M., Beck-Sickinger,A.G., Holst,B. In Vivo Characterization of High Basal Signaling from the Ghrelin Receptor. Endocrinology 2009; 150(-11-):4920-4930. >>> Substance P; PBS; BSA; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle or inactive control peptide; animal info (Male Wistar, 275-300 g, STZ induced diabetes); peptides.

P9691 Popiel,H.A., Nagai,Y., Fujikake,N., Toda,T. Delivery of the aggregate inhibitor peptide QBP1 into the mouse brain using PTDs and its therapeutic effect on polyglutamine disease mice. Neuroscience Letters 2009; 449(-2-):87-92. >>> PolyQ-binding peptide 1, Antp-; CSF/CNS; Mice (transgenic); ; Peptides; animal info (4 wks old, R6/2); neurodegenerative (Polyglutamine Disease); brain tissue distribution.

Q0384 Quesada,A., Micevych,P., Handforth,A. C-terminal mechano growth factor protects dopamine neurons: A novel peptide that induces heme oxygenase-1. Experimental Neurology 2009; 220(-2-):255-266. >>> Mechano growth factor 24; JB1; CSF, artificial; CSF/CNS; SC; Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (adult, Long-Evans, male, 225-250 g); peptides; JB1 is an IGF-1 analog.

P9689 Srivareerat,M., Tran,T.T., Alzoubi,K.H., Alkadhi,K.A. Chronic Psychosocial Stress Exacerbates Impairment of Cognition and Long-Term Potentiation in beta-Amyloid Rat Model of Alzheimer's Disease. Biological Psychiatry 2009; 65(-11-):918-926. >>> Amyloid protein, beta (1-40); amyloid protein, beta (1-42); Water, distilled; acetonitrile; trifluoroacetate; CSF/CNS; Rat; 2 weeks; Controls received mp w/ inactive reverse peptide, amyloid protein beta (40-1); post op. care (antibiotic ointment); animal info (male, Wistar, 200-225 g.); neurodegenerative (Alzheimer's Disease).

P9806 Tang,H.D., Wang,Y.M., Xie,L., Mao,X.O., Won,S.J., Galvan,V., Jin,K.L. Effect of neural precursor proliferation level on neurogenesis in rat brain during aging and after focal ischemia. NEUROBIOLOGY OF AGING 2009; 30(-2-):299-308. >>> Z-DEVD-FMK; DMSO; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; enzyme inhibitor (peptide methylketone caspase-3); animal info (5 days old, 3, 11, 24, months old, Fischer 344); brain tissue distribution.

P9709 Thompson,E.L., Amber,V., Stamp,G.W.H., Patterson,M., Curtis,A.E., Cooke,J.H., Appleby,G.F., Dhillo,W.S., Ghatei,M.A., Bloom,S.R., Murphy,K.G. Kisspeptin-54 at high doses acutely induces testicular degeneration in adult male rats via central mechanisms. British Journal of Pharmacology 2009; 156(-4-):609-625. >>> Kisspeptin-54; Saline; SC; CSF/CNS; Rat; 2001D; 2001; 1, 2, 3 days; 6, 12, 24 hours; Controls received mp w/vehicle; functionality of mp verified; peptides; animal info (adult, male, Wistar, 275-325 g.).

P9639 van Groen,T., Kadish,I., Wiesehan,K., Funke,S.A., Willbold,D. In vitro and in vivo Staining Characteristics of Small, Fluorescent, A-beta-42-Binding D-Enantiomeric Peptides in Transgenic AD Mouse Models. ChemMedChem 2009; 4(-2-):276-282. >>> FITC-D1; FITC-D3; CSF/CNS (hippocampus); Mice (transgenic); 2004; 4 weeks; Controls received mp w/saline; ALZET brain infusion kit 3 used; peptides; animal info (8 mo old, AP/PS-delta; neurdegenerative (Alzheimer's disease); D1 and D3 are D-amino acid peptides developed specifically to bind to amyloid -beta (1-42); brain tissue distribution.

P9459 Wei,S.G., Yu,Y., Zhang,Z.H., Felder,R.B. Angiotensin II upregulates hypothalamic AT1 receptor expression in rats via the mitogen-activated protein kinase pathway. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1425-H1433. >>> Angiotensin II; losartan; PD-98059; SP-600125; SB-203580; Saline; DMSO; CSF, artificial; SC; CSF/CNS; Rat; 2002; 2004; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; enzyme inhibitor (MAP kinase, Jun N-terminal kinase); cardiovascular; peptides; multiple pumps per animal (2); animal info (male, Sprague Dawley, 275-300 g.); cannula placement confirmed at end of study; 0.1%-0.5% DMSO used.

P9462 White,C.L., Whittington,A., Barnes,M.J., Wang,Z., Bray,G.A., Morrison,C.D. HF diets increase hypothalamic PTP1B and induce leptin resistance through both leptin-dependent and -independent mechanisms. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2009; 296(-2-):E291-E299. >>> Leptin; Saline; SC; CSF/CNS; Rat; mice; 10, 14 days; Controls received mp w/ vehicle; comparison of IP, ICV injections vs. mp; peptides; post op. care (analgesics); animal info (male, Long Evans, 10 wks old; C57BL/6 wt, ob/ob, 8 wks old).

P9971 Xue,B.J., Singh,M., Guo,F., Hay,M., Johnson,A.K. Protective actions of estrogen on angiotensin II-induced hypertension: role of central nitric oxide. American Journal of Physiology-Heart and Circulatory Physiology 2009; 297(-5-):H1638-H1646. >>> L-NAME; L-VNIO; Angiotensin II; SC; CSF/CNS; Mice; 1002; 7, 14 days; Controls received mp w/ vehicle; peptides; cardiovascular; enzyme ihibitor (NO synthase); animal info (male, female, C57BL/6, 12-16 weeks old); replacement therapy (ovariectomy, gonadectomy); cannula placement confirmed post mortem histologically; N5-(1-imino-3-butenyl)-L-ornithine (L-VNIO).

P9968 Zhang,Q., Yao,F.R., O'Rourke,S.T., Qian,S.Y., Sun,C.W. Angiotensin II enhances GABAB receptor-mediated responses and expression in nucleus tractus solitarii of rats. American Journal of Physiology-Heart and Circulatory Physiology 2009; 297(-5-):H1837-H1844. >>> Angiotensin II; CSF/CNS; Rat; 2004; 7 days; Controls received mp w/ aCSF; peptides; cardiovascular; ALZET brain infusion kit 2 used; animal info (male, Sprague-Dawley, 290-350 g); functionality of mp verified by mean arterial BP.

P9386 Audoy-Remus,J., Richard,J.F., Soulet,D., Zhou,H., Kubes,P., Vallieres,L. Rod-Shaped Monocytes Patrol the Brain Vasculature and Give Rise to Perivascular Macrophages under the Influence of Proinflammatory Cytokines and Angiopoietin-2. Journal of Neuroscience 2008; 28(-41-):10187-10199. >>> L1-10; PBS; CSF/CNS; Mice; 2001; 7 days; Controls received mp w/ vehicle; comparison of IV injections vs. mp; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 2-3 months old); L1-10 is an anti-Angpt2-peptide-Fc fusion protein.

P8839 Augustine,R.A., Grattan,D.R. Induction of central leptin resistance in hyperphagic pseudopregnant rats by chronic prolactin infusion. Endocrinology 2008; 149(-3-):1049-1055. >>> Prolactin, ovine; CSF, artificial; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (female, Sprague Dawley, 10 wks old, 200-300g., pseudopregnant); endocrinology.

P9324 Averill,S., Inglis,J.J., King,V.R., Thompson,S.W.N., Cafferty,W.B.J., Shortland,P.J., Hunt,S.P., Kidd,B.L., Priestley,J.V. Reg-2 expression in dorsal root ganglion neurons after adjuvant-induced monoarthritis. Neuroscience 2008; 155(-4-):1227-1236. >>> Nerve growth factor, recomb. human; glial-derived neurotrophic factor, recomb. human; leukemia inhibitory factor, recomb. human; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides, animal info (male, Wistar, 220-400 g.).

P9392 Bosch,O.J., Neumann,I.D. Brain vasopressin is an important regulator of maternal behavior independent of dams' trait anxiety. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-44-):17139-17144. >>> Vasopressin, arginine; oxytocin; oligonucleotide, antisense; oligonucleotide, missense; Ringer's solution; CSF/CNS; CSF/CNS (medial preoptic area); Rat (pregnant); 1007D; 5-7 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; antisense (AVP-R); peptides; multiple pumps per animal (2); post op. care (Enrofloxacin, Baytril); animal info (female, Wistar, HAB, LAB, 14 wks old); behavioral testing (maternal care, pup retrieval test, elevated plus maze); cannula placement and infusion sites verified with blue ink.

P9323 Burgos-Ramos,E., Puebla-Jimenez,L., rilla-Ferreiro,E. Minocycline provides protection against beta-amyloid(25-35)-induced alterations of the somatostatin signaling pathway in the rat temporal cortex. Neuroscience 2008; 154(-4-):1458-1466. >>> Amyloid protein, beta (25-35); Acetic acid; CSF/CNS; Rat; 14 days; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, 200-250g.); neurodegenerative (Alzheimer's Disease).

P9124 Cao,Y., Shumsky,J.S., Sabol,M.A., Kushner,R.A., Strittmatter,S., Hamers,F.P.T., Lee,D.H.S., Rabacchi,S.A., Murray,M. Nogo-66 receptor antagonist peptide (NEP1-40) administration promotes functional recovery and axonal growth after lateral funiculus injury in the adult rat. NEUROREHABILITATION AND NEURAL REPAIR 2008; 22(-3-):262-278. >>> NEP1-40; DMSO; PBS; CSF/CNS (intrathecal); Rat; 2004; ; Controls received mp w/ vehicle; peptides, animal info (female, Sprague Dawley); 2.5% DMSO used; compound is a Nogo-66 receptor antagonist peptide; good methods (pg. 263).

Q0036 Chang,M., Cooper,J.D., Sleat,D.E., Cheng,S.H., Dodge,J.C., Passini,M.A., Lobel,P., Davidson,B.L. Intraventricular enzyme replacement improves disease phenotypes in a mouse model of late infantile neuronal ceroid lipofuscinosis. MOLECULAR THERAPY 2008; 16(-4-):649-656. >>> Tripeptidyl peptidase 1, recomb human; PBS; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; peptides; brain tissue distribution; ALZET brain infusion kit 3 used; neurodegenerative (LINCL); animal info (C57BL/6, CLN2-/-, 60 days old); cannula placement confirmed visually.

P9732 Chen,Z.L., Yu,H.X., Yu,W.M., Pawlak,R., Strickland,S. Proteolytic fragments of laminin promote excitotoxic neurodegeneration by up-regulation of the KA1 subunit of the kainate receptor. Journal of Cell Biology 2008; 183(-7-):1299-1313. >>> Laminin-1, mouse purified; Immunoglobulin G, rabbit; Laminin-1, plasmin digested; Antibody, anti-KA1; CSF/CNS (hippocampus); Mice; 1007D; ; Controls received mp w/vehicle, or PBS; peptides; animal info (adult, male, Lam-gamma-1KO); brain tissue distribution.

P9402 de Chevigny,A., Cooper,O., Vinuela,A., Reske-Nielsen,C., Lagace,D.C., Eisch,A.J., Isacson,O. Fate mapping and lineage analyses demonstrate the production of a large number of striatal neuroblasts after transforming growth factor alpha and noggin striatal infusions into the dopamine-depleted striatum. Stem Cells 2008; 26(-9-):2349-2360. >>> Transforming growth factor-a; noggin; PBS; CSF/CNS (striatum); Rat; 1002; 2004; 14, 28, 41 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; brain tissue distribution; peptides; animal info (female, Sprague Dawley, 250-300 g.); behavioral testing (rotation behavior); noggin is a bone morphogenetic protein antagonist; neurodegenerative (Parkinson's Disease).

P9320 De Toni,A., Zbinden,M., Epstein,J.A., Altaba,A.R.I., Prochiantz,A., Caille,I. Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP. Neural Development 2008; 3(-;-):U2-U13. >>> Epidermal growth factor; fibroblast growth factor, basic; RNA, small interfering; Saline, physiological; CSF/CNS; CSF/CNS (dentate gyrus); Rat; 1003D; 3 days; Controls received mp w/ control siRNA; cancer (glioblastoma); peptides; animal info (Swiss, HOP -/-, wt, adult); HOP or control siRNA was coupled to the cell permeant peptide, penetratin.

P8910 do Carmo,J.M., Hall,J.E., da Silva,A.A. Chronic central leptin infusion restores cardiac sympathetic-vagal balance and baroreflex sensitivity in diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-5-):H1974-H1981. >>> Leptin; CSF/CNS; Rat; 1007D; 7 days; Peptides; animal info (male, Sprague Dawley, 340-360 g.); tygon tubing used; diabetes.

R0247 Fernstrom,J.D., Choi,S. The development of tolerance to drugs that suppress food intake. PHARMACOLOGY&THERAPEUTICS 2008; 117(-1-):105-122. >>> Fenfluramine; leptin; melanotan II; CSF, artificial; PBS; SC; CSF/CNS; Rat; 8 weeks; 7, 28 days; Controls received mp w/ vehicle; dose-response (fig. 5); pumps replaced; tolerance; peptides; animal info (male, obese); endocrinology; review, see p. 108-110.

P9237 Galvao,R.P., Garcia-Verdugo,J.M., varez-Buylla,A. Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats. Journal of Neuroscience 2008; 28(-50-):13368-13383. >>> Ara-C; brain-derived neurotrophic factor; Saline; BSA; CSF/CNS; Rat; mice; 1007D; 2002; 6, 14 days; Controls received mp w/ vehicle; functionality of mp verified by weight loss due to BDNF's appetite suppression (p. 13377), residual volume; stability verified by testing activity of residual BDNF volume after 14 days/37 degrees Celsius; brain tissue distribution; peptides; post op. care (Buprenorphine); animal info (male, Sprague Dawley, 250 g., male CD-1 3 mo old); mp/cannula placement visually confirmed after brain sectioning.

P9315 Gleason,D., Fallon,J.H., Guerra,M., Liu,J.C., Bryant,P.J. Ependymal stem cells divide asymmetrically and transfer progeny into the subventricular zone when activated by injury. Neuroscience 2008; 156(-1-):81-88. >>> Transforming growth factor-a; PBS; CSF/CNS (caudate putamen); Rat; 1, 3, 5, 7, 28 days; Peptides, animal info (male, 10 wks old); neurodegenerative (Parkinson's Disease); neural stem cell research.

P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.

P9227 Hashimoto,M., Shahdat,H.M., Yamashita,S., Katakura,M., Tanabe,Y., Fujiwara,H., Gamoh,S., Miyazawa,T., Arai,H., Shimada,T., Shido,O. Docosahexaenoic acid disrupts in vitro amyloid beta (1-40) fibrillation and concomitantly inhibits amyloid levels in cerebral cortex of Alzheimer's disease model rats. Journal of Neurochemistry 2008; 107(-6-):1634-1646. >>> Amyloid protein, beta (1-40); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; ; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, 20 wks old, 384 g.); neurodegenerative (Alzheimer's Disease).

P8853 Hook,V.Y.H., Kindy,M., Hook,G. Inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant beta-secretase site of the amyloid precursor protein. Journal of Biological Chemistry 2008; 283(-12-):7745-7753. >>> CAO74Me; E64d; Saline; Me2SO; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; no stress (see pg. 7747); enzyme inhibitor (cysteine protease); ALZET brain infusion kit 3 used; peptides; animal info (male, London APP, Swedish/London APP; 6, 12 months old); neurodegenerative (Alzheimer's disease); "Animals remained healthy and were observed to have normal eating, body weight, grooming + general appearance during the inhibitor treatment period. Of the 96 animals used in this study, none showed postoperative complications, such as infections; also, none died." (p. 7747).

P9273 Ito,M., Gomori,A., Suzuki,J., Tsujioka,S., Sasaki,M., Matsuda,M., Bednarek,M.A., Ito,M., Ishihara,A., Iwaasa,H., MacNeil,D.J., Kanatani,A. Antagonism of central melanin-concentrating hormone 1 receptor alleviates steatohepatitis in mice. Journal of Endocrinology 2008; 198(-2-):309-315. >>> Melanin-concentrating hormone 1, receptor antagonist; Water, distilled; propylene glycol; CSF/CNS; Mice; 2004; 4, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 2 or 4 weeks; no stress (see pg. 311, 314); ALZET brain infusion kit used; peptides; post op. care (Cefamezin); animal info (male, C57BL/6, 10 wks old, 16 wks old, 1 year old); animals received mp w/vehicle for 2 or 4 weeks, then mp with agent or vehicle for 2 or 4 more weeks; dental cement used; cannula placement confirmed at end of experiment with Evans blue dye.

P9486 Johnson,M.A., Fraley,G.S. Rat RFRP-3 Alters Hypothalamic GHRH Expression and Growth Hormone Secretion but Does Not Affect KiSS-1 Gene Expression or the Onset of Puberty in Male Rats. Neuroendocrinology 2008; 88(-4-):305-315. >>> Oligonucleotide, antisense; oligonucleotide, mismatch; NPFF; RFamide-related peptide-3; PBS; CSF/CNS; CSF/CNS (third ventricle); Rat; 2002; 7, 14 days; Controls received mp w/ vehicle or mismatch ODN; functionality of mp verified; antisense (RFRP-3); ALZET brain infusion kit used; peptides; animal info (male, Sprague Dawley, 24 days old, 60 g., 35 days old); endocrinology; cannula placement confirmed histologically at end of experiment.

P9053 Kang,Y.M., Ma,Y., Elks,C., Zheng,J.P., Yang,Z.M., Francis,J. Cross-talk between cytokines and renin-angiotensin in hypothalamic paraventricular nucleus in heart failure: role of nuclear factor-kappa B. Cardiovascular Research 2008; 79(-4-):671-678. >>> Losartan; SN50; tempol; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; peptides; animal info (male, Sprague Dawley, 250-275 g.); pumps were primed in 0.9% saline for 24 hours; SN50 is a synthetic protein.

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P8797 Lee,M., Kim,A., Conwell,I.M., Hruby,V., Mayorou,A., Cai,M.M., Wardlaw,S.L. Effects of selective modulation of the central melanocortin-3-receptor on food intake and hypothalamic POMC expression. Peptides 2008; 29(-3-):440-447. >>> PG-932; [D-Trp8]-gamma-MSH; Saline; CSF/CNS; Rat; 2001; 1003D; 3, 7 days; 84, 66 hours; Controls received mp w/ vehicle; dose-response (fig 4, pg 445); pumps replaced after 7 days; peptides; animal info (male, Sprague-Dawley, 250-275 grams); agents are melanocortin-3-receptor agonists and antagonists ([D-Trp8]-gamma-MSH and PG-932 respectively).

P8934 Lu,J., Zhang,Y.D., Shi,J.P. Effects of intracerebroventricular infusion of angiotensin-(1-7) on bradykinin formation and the kinin receptor expression after focal cerebral ischemia-reperfusion in rats. Brain Research 2008; 1219(-;-):127-135. >>> Angiotensin (1-7); CSF, artificial; CSF/CNS; Rat; 1007D; ; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptide; ischemia (cerebral); animal info (male, Sprague Dawley, 280-300 g.); MCAO.

P9377 Miyoshi,K., Obata,K., Kondo,T., Okamura,H., Noguchi,K. Interleukin-18-Mediated Microglia/Astrocyte Interaction in the Spinal Cord Enhances Neuropathic Pain Processing after Nerve Injury. Journal of Neuroscience 2008; 28(-48-):12775-12787. >>> Immunoglobulin G, goat; antibody, anti-interleukin-18; antibody, anti-leukin-18 receptor; interleukin-18 binding protein; RNA, small interfering; PBS; CSF/CNS (intrathecal); Rat; mice; 1003D; 2001; 3, 7 days; Controls received mp w/ vehicle or IgG or negative siRNA; peptides; animal info (male, Sprague Dawley, 200-250 g.; male, C57BL/6 wt, IL-18 -/-, 25-30 g.); siRNA targets TLR4 (toll-like receptor 4) or negative siRNA; behavioral testing (mechanical hypersensitivity, tactile allodynia).

P9209 Ohta,Y., Kamiya,T., Nagai,M., Nagata,T., Morimoto,N., Miyazaki,K., Murakami,T., Kurata,T., Takehisa,Y., Ikeda,Y., Asoh,S., Ohta,S., Abe,K. Therapeutic benefits of intrathecal protein therapy in a mouse model of amyotrophic lateral sclerosis. Journal of Neuroscience Research 2008; 86(-13-):3028-3037. >>> TAT-GFP; TAT-Bcl-XL; CSF, artificial; CSF/CNS (intrathecal); Mice; mice (transgenic); 2001; 2004; 7, 28 days; Controls received mp w/ vehicle; stability verified by antiapoptotic effect at 7, 14, and 28 days at 37 degree Celsius (p. 3031); peptides; animal info (male, B6SJL, wt, G93A SOD1 Tg, 91 days old); neurodegenerative (ALS); behavioral testing (Rotarod test, wheel running test); "intrathecal infusion of TAT-infused proteins using an osmotic minipump is a better method of delivering therapeutic proteins into the spinal cord." (p. 3035).

P8800 Park,S., Hong,S.M., Sung,S.R., Jung,H.K. Long-term effects of central leptin and resistin on body weight, insulin resistance, and beta-cell function and mass by the modulation of hypothalamic leptin and insulin signaling. Endocrinology 2008; 149(-2-):445-454. >>> Leptin, recomb. rat; resistin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; replacement therapy (pancreatectomy, 90%); peptides; animal info (male, Sprague Dawley, 220g).

P8803 Pinnock,S.B., Herbert,J. Brain-derived neurotropic factor and neurogenesis in the adult rat dentate gyrus: interactions with corticosterone. European Journal of Neuroscience 2008; 27(-10-):2493-2500. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, rat serum; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 2496) 1 animal died (cause unknown); peptides; animal info (male, Lister-Hooded, 200-250g.); cannula position confirmed post mortem with cresyl violet.

P9437 Riazi,K., Galic,M.A., Kuzmiski,J.B., Ho,W., Sharkey,K.A., Pittman,Q.J. Microglial activation and TNF alpha production mediate altered CNS excitability following peripheral inflammation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-44-):17151-17156. >>> Tumor necrosis factor-a; Saline; albumin, rat; chlorbutanol; CSF/CNS; Rat; 1007D; 4 days; Controls received mp w/ vehicle; peptides; animal info (male, Sprague Dawley, 175-200 g.).

P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).

P9439 Sahu,A. Effects of chronic central leptin infusion on proopiomelanocortin and neurotensin gene expression in the rat hypothalamus. Neuroscience Letters 2008; 440(-2-):125-129. >>> Leptin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 2002; 23 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; pumps replaced after 7 days of mp w/aCSF alone for 16 more days of vehicle or agent; peptides; animal info (male, Sprague Dawley, 250 g.).

P9441 Sakai,A., Asada,M., Seno,N., Suzuki,H. Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain. Pain 2008; 137(-2-):378-388. >>> Oligodeoxynucleotide, phosphorothioate antisense; glial-derived neurotrophic factor, recomb. human; neural cell adhesion molecule, mimetic peptide C3d; oligodeoxynucleotide, phosphorothioate missense; CSF/CNS (subarachnoid space); Rat; 4, 6, 7, 9 days; Controls received mp w/ saline; antisense (NCAM); peptides; multiple pumps per animal (2); animal info (male, Sprague Dawley, 5 wks old, 140-160 g., sciatic nerve injury); behavioral testing (mechanical allodynia, thermal hypersensitivity, paw withdrawal, plantar test).

P9443 Segi-Nishida,E., Warner-Schmidt,J.L., Duman,R.S. Electroconvulsive seizure and VEGF increase the proliferation of neural stem-like cells in rat hippocampus. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-32-):11352-11357. >>> Vascular endothelial growth factor 164, recomb. rat; PBS; CSF/CNS; Rat; 1003D; 3 days; 24 hours; Controls received mp w/ vehicle; brain tissue distribution; peptides; animal info (male, Sprague Dawley, 175-200 g.); delayed delivery (in the 48 hour infusion, PBS infused for 24 hours to "avoid the influence of cannulation and anesthesia." (p. 11354) then VEGF infused for 24 hours).

P9447 Song,X.Y., Li,F., Zhang,F.H., Zhong,J.H., Zhou,X.F. Peripherally-Derived BDNF Promotes Regeneration of Ascending Sensory Neurons after Spinal Cord Injury. PLoS One 2008; 3(-3-):U22-U37. >>> Brain-derived neurotrophic factor, recomb. human; CSF/CNS (sciatic nerve); Rat; 1007D; 7 days; Controls received mp w/ PBS; comparison of foot pad injections vs. mp; peptides; animal info (female, Sprague Dawley, 8-10 wks old, SCI); behavioral testing (hind limb motor function).

P9223 Sutton,G.M., Perez-Tilve,D., Nogueiras,R., Fang,J.D., Kim,J.K., Cone,R.D., Gimble,J.M., Tschoep,M.H., Butler,A.A. The Melanocortin-3 Receptor Is Required for Entrainment to Meal Intake. Journal of Neuroscience 2008; 28(-48-):12946-12955. >>> Agouti-related peptide (82-131); CSF, artificial; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; dental cement and stay screws used; peptides; post op. care (Buprenorphine); animal info (male, B6, 8 wks old).

P9298 Takeo,S., Takagi,N., Takagi,K., Date,I., Ishida,K., Besshoh,S., Nakamura,T., Tanonaka,K. Hepatocyte growth factor suppresses ischemic cerebral edema in rats with microsphere embolism. Neuroscience Letters 2008; 448(-1-):125-129. >>> Hepatocyte growth factor, recomb. human; Saline, physiological; CSF/CNS; Rat; 2001; 3 days; Controls received no treatment; peptides; ischemia (cerebral); animal info (male, Wistar, 220-250 g.).

P9456 Ufnal,M., Sikora,M., Szczepanska-Sadowska,E. Interleukin-1 receptor antagonist reduces the magnitude of the pressor response to acute stress. Neuroscience Letters 2008; 448(-1-):47-51. >>> Interleukin-1, beta, recomb. rat; interleukin-1 receptor antagonist, recomb. rat; CSF/CNS; Rat; 2001; 5 days; Controls received mp w/ saline; cardiovascular; ALZET brain infusion kit 1 used; peptides; post op. care (benzalathine penicycline); animal info (male, Wistar-Kyoto, 12 wks old).

P9721 van Groen,T., Wiesehann,K., Funke,S.A., Kadish,I., Nagel-Steger,L., Willbold,D. Reduction of Alzheimer's Disease Amyloid Plaque Load in Transgenic Mice by D3, a D-Enantiomeric Peptide Identified by Mirror Image Phage Display. ChemMedChem 2008; 3(-12-):1848-1852. >>> FITC-D3; FITC-D1; CSF/CNS (hippocampus); Mice (transgenic); 31 days; Controls received mp w/D1, or saline; peptides; animal info (APPswe/PS1) E9; neurodegenerative (Alzheimer's disease); D-3 is a novel D-enantiomeric amino acid peptide.

P9798 Viel,T.A., Caetano,A.L., Nasello,A.G., Lancelotti,C.L., Nunes,V.A., Araujo,M.S., Buck,H.S. Increases of kinin B1 and B2 receptors binding sites after brain infusion of amyloid-beta 1-40 peptide in rats. NEUROBIOLOGY OF AGING 2008; 29(-12-):1805-1814. >>> Amyloid protein, beta (1-40), human; E-64; CSF/CNS; Rat; 2004; 5 weeks; Controls received mp w/ enzyme inhibitor (cystein protease); ALZET brain infusion kit 1 used; peptides; animal info (male, Wistar, 200-250 g.); neurodegenerative (Alzheimer's Disease).

P9074 Xue,B.J., Zhao,Y.Z., Johnson,A.K., Hay,M. Central estrogen inhibition of angiotensin II-induced hypertension in male mice and the role of reactive oxygen species. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1025-H1032. >>> Tempol; estradiol, 17B-; ICI-182780; SC; CSF/CNS; Mice; 1002; 7, 14 days; ALZET brain infusion kit used; peptides; animal info (male, C57BL/6, 12-16 wks old); location of cannula was verified histologically post mortem.

P8760 Yao,F.R., Sumners,C., O'Rourke,S.T., Sun,C.W. Angiotensin II increases GABAB receptor expression in nucleus tractus solitarii of rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-6-):H2712-H2720. >>> Angiotensin II; CSF/CNS; Rat; 1002; 2 weeks; Controls received mp w/ physiological saline; cardiovascular; peptides; animal info (male, Sprague Dawley 270-320g).

P8829 Zaheer,A., Zaheer,S., Thangavel,R., Wu,Y.H., Sahu,S.K., Yang,B.L. Glia maturation factor modulates beta-amyloid-induced glial activation, inflammatory cytokine/chemokine production and neuronal damage. Brain Research 2008; 1208(-;-):192-203. >>> Amyloid protein, beta (1-42), human; Saline; CSF/CNS; Mice; 2004; 28 days; Controls received mp w/ vehicle; peptides; animal info (C57BL/6, wt, GMF-KO, 5-6 months old); neurodegenerative (Alzheimer's disease).