References on the Administration of Peptides to the CNS Using ALZET® Osmotic Pumps

16332 Sophie Dutheil,, Isabelle Watabe,, Karina Sadlaoud,, Alain Tonetto,, Brahim Tighilet2  Journal of Neuroscience 2016; 36(-23-):6199-6212. >>> Brain-derived neurotrophic factor, K252a; Saline; CSF, artificial; CSF/CNS (ventricles); Cat; 2ML4; Controls received mp w/ saline; animal info (adult, male cat, 4-5 kg); dose-response (pg 6205, 6206); behavioral testing (rotating beam experimental device test); peptides;.

16330 Drougard, A., Fournel, A., Marlin, A., Meunier, E., Abot, A., Bautzova, T., Duparc, T., Louche, K., Batut, A., Lucas, A., Le-Gonidec, S., Lesage, J., Fioramonti, X., Moro, C., Valet, P., Cani, P. D., Knauf, C. Central chronic apelin infusion decreases energy expenditure and thermogenesis in mice. Sci Rep 2016; 6(--):31849. >>> Apelin; CSF, artificial; CSF/CNS (lateral ventricle); Mice; 2004; Controls received mp w/ vehicle; animal info (C57Bl6/J mice, 13-15 week old); animal info (C57Bl6/J mice, 13-15 week old);“stability of apelin and the duration of the treatment were compatible with the stability of the molecule” (pg. 8); peptides; anesthetised mice with isoflurane; Brain coordinates − 1 mm lateral, − 0.2 mm anteroposterior from the bregma and − 1.7 mm deep; Dose (20 nM);.

16230 Pikor, N. B., Astarita, J. L., Summers-Deluca, L., Galicia, G., Qu, J., Ward, L. A., Armstrong, S., Dominguez, C. X., Malhotra, D., Heiden, B., Kay, R., Castanov, V., Touil, H., Boon, L., O'Connor, P., Bar-Or, A., Prat, A., Ramaglia, V., Ludwin, S., Turley, S. J., Gommerman, J. L. Integration of Th17- and Lymphotoxin-Derived Signals Initiates Meningeal-Resident Stromal Cell Remodeling to Propagate Neuroinflammation. Immunity 2015; 43(-6-):1160-73. >>> Anti-interleukin 22, anti-interleukin 17, LymphotoxinǞR-Ig; Immunoglobulin; CSF/CNS (cerebroventricular); mice; 2004; Controls received mp w/ vehicle; animal info (SJL/J mice,); animal info (SJL/J mice,); dose-response (pg 1166); peptides; Fibroblast remodeling in vivo; Dose (2 mg/ml (LTBR), 2.25 mg/ml (IL));.

16229 Perianes-Cachero, A., Canelles, S., Aguado-Llera, D., Frago, L. M., Toledo-Lobo, M. V., Carrera, I., Cacabelos, R., Chowen, J. A., Argente, J., Arilla-Ferreiro, E., Barrios, V. Reduction in Abeta-induced cell death in the hippocampus of 17beta-estradiol-treated female rats is associated with an increase in IGF-I signaling and somatostatinergic tone. J Neurochem 2015; 135(-6-):1257-71. >>> Amyloid protein, beta (25-35); ICI 182,780; saline; CSF/CNS (right cerebral ventricle); rat; 2002; Controls received mp w/ vehicle; animal info (female Wistar rats, 8 weeks); brain infusion kit used; peptides; Amyloid beta-peptide induces cell death, learning and memory, hippocampal somatostatinergic system; Dose (300 pmol/day (A-beta), 600 fmol/day (ICI)); Brain coordinates; (-0.3 mm anteroposterior, 1.1 mm lateral).

16196 Lee, Chun-Lin, Lin, Pei-Ying, Hsu, Ya-Wen, Pan, Tzu-Ming  Journal of Functional Foods 2015; 18(--):387-399. >>> Amyloid protein, beta (40); Acetonitrile, trifluoroacetic acid; CSF/CNS (left ventricle); rat; 2004; Controls received mp w/ vehicle; animal info: Male Sprague Dawley (SD) rats at 6–8 weeks; functionality of mp verified by behavior tests; 35% acetonitrile, 0.1% trifluoroacetic acid; dose-response (pg 390); behavioral testing (Morris water maze; reference memory task, probe test, and working memory task); brain tissue distribution; peptides; cannula was inserted 4.0 mm into the left ventricle and attached to the skull with dental cement; Dose: 0.28 uL/h.

16193 Kim, J. S., Brownjohn, P. W., Dyer, B. S., Beltramo, M., Walker, C. S., Hay, D. L., Painter, G. F., Tyndall, J. D., Anderson, G. M. Anxiogenic and Stressor Effects of the Hypothalamic Neuropeptide RFRP-3 Are Overcome by the NPFFR Antagonist GJ14. Endocrinology 2015; 156(-11-):4152-62. >>> Peptide-3, RF amide-related; GJ14; CSF/CNS (left lateral ventricle); mice; 1007D; Controls received mp w/ vehicle; animal info: Female C57BL/6 mice (8 wk old); functionality of mp verified by behavior testing; behavioral testing (elevated plus maze and open field tests for 5 minutes each); peptides; RFRP-3 aka Peptide-3, RF amide-related; GJ14 is a NPFF1R antagonist with moderate antagonism at NPFF2R; G-protein coupled neuropeptide FF (NPFF) receptors belong to the RFamide receptor family and are referred to as NPFF1R (also known as GPR147) and NPFF2R (GPR74); Dose: 2 nmol/mouse/d (RFRP-3); 20 nmol/mouse/d (GJ14).

16185 Su, C. H., Tsai, C. Y., Chang, A. Y., Chan, J. Y., Chan, S. H. MRI/DTI of the Brain Stem Reveals Reversible and Irreversible Disruption of the Baroreflex Neural Circuits: Clinical Implications. Theranostics 2016; 6(-6-):837-48. >>> Angiotensin II; CSF/CNS (ventricle); Mice; 1007D; animal info (male, C57BL6, adult); cardiovascular; peptides; bp measured using radiotelemetry (DSI); Dose (7.5 ug/hr);.

16129 Giridharan, V. V., Thandavarayan, R. A., Arumugam, S., Mizuno, M., Nawa, H., Suzuki, K., Ko, K. M., Krishnamurthy, P., Watanabe, K., Konishi, T. Schisandrin B Ameliorates ICV-Infused Amyloid beta Induced Oxidative Stress and Neuronal Dysfunction through Inhibiting RAGE/NF-kappaB/MAPK and Up-Regulating HSP/Beclin Expression. PLoS One 2015; 10(-11-):e0142483. >>> Amyloid protein, beta (1-40); Acetonitrile; trifluoroacetic acid; CSF/CNS; rat; 2002; Controls received mp w/ vehicle; animal info: Male Sprague Dawley rats, 240–300 g; functionality of mp verified by brain removal and analysis; colorimetric sandwich ELISA kit; %35 of acetonitrile; 0.1% triluoroacetic acid; dose-response ( pg 7); neurodegenerative (memory impairment); behavioral testing (Passive avoidance task (PAT)); peptides; Dose: 300 pmol/day; Brain coordinates; relative to the bregma (A, 0.8; L, 1.4; V, 4.5) according to the atlas of Paxinos and Watson.

16113 Dorr, A., Kress, E., Podschun, R., Pufe, T., Tauber, S. C., Brandenburg, L. O. Intrathecal application of the antimicrobial peptide CRAMP reduced mortality and neuroinflammation in an experimental model of pneumococcal meningitis. J Infect 2015; 71(-2-):188-99. >>> Antimicrobial peptide, cathelicidin-related; Saline; CSF/CNS (right lateral ventricle); mice; 1007D; Controls received mp w/ saline; animal info: male C57BL/6 mice (weight 19-23 g, aged 2-3 months); ALZET brain infusion kit 2 used; good methods (pg 189-190); brain tissue distribution of CRAMP; peptides; CRAMP aka Cathelicidin-related antimicrobial peptide; Dose: 12 ug/d.

16053 Uekawa, K., Hasegawa, Y., Senju, S., Nakagata, N., Ma, M., Nakagawa, T., Koibuchi, N., Kim-Mitsuyama, S. Intracerebroventricular Infusion of Angiotensin-(1-7) Ameliorates Cognitive Impairment and Memory Dysfunction in a Mouse Model of Alzheimer's Disease. J Alzheimers Dis 2016; 53(-1-):127-33. >>> Angiotensin (1-7); A-779; CSF, artificial; CSF/CNS; Mice; 1004; Controls received mp w/ vehicle; animal info (male, 5XFAD, 17 months old); ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer’s Disease); behavioral testing (Morris water maze); cardiovascular; peptides; used ALZET CSF formulation; Dose (Ang 1-7 500 ng/kg/h; A-779 5.0 ug/kg/h); Brain coordinates (relation to bregma 1.0mm lateral and 0.5 mm posterior);.

15984 Ko, A. R., Hyun, H. W., Min, S. J., Kim, J. E., Kang, T. C. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 2015; 8(--):58. >>> BQ-788; Cav-1 peptide; Y-27632; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley); ALZET brain infusion kit 1 used; peptides; brain coordinates;.

15916 Kim, D. H., Moon, E. Y., Yi, J. H., Lee, H. E., Park, S. J., Ryu, Y. K., Kim, H. C., Lee, S., Ryu, J. H. Peptide fragment of thymosin beta4 increases hippocampal neurogenesis and facilitates spatial memory. Neuroscience 2015; 310(--):51-62. >>> Ac-SDKP; PBS; CSF/CNS (third ventricle); Mice; 1007D; animal info (male, C57BL6J, 25-28g, 8 weeks old); behavioral testing (Morris water maze); tissue perfusion (third ventricle); peptides; Cannula placement verified via histologic analysis; used Plastics one cannula; brain coordinates;.

15847 Baojian Xue, Robert L. Thunhorst, Yang Yu, Fang Guo, Terry G. Beltz, Robert B. Felder,, Alan Kim Johnson  Hypertension 2016; 67(--):163-170. >>> Pentoxifylline; irbesartan; minocycline; angiotensin II; CSF, artificial; Na sodium bicarbonate; saline; CSF/CNS; Rat; 2004; 2002; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 10-12 weeks old); cardiovascular; peptides; Dose (ICV - Pentoxifylline 10 ug/hr; irbesartan 125 ug/day; minocycline 5 ug/hr; SC AngII 120 ng/kg/min); brain coordinates;.

15837 Renjun Wang, PhD; Qian Huang, MD; Rui Zhou, BSc; Zengxiang Dong, PhD;, Yunfeng Qi, PhD; Hua Li, MS; Xiaowei Wei, MS; Hui Wu, MS; Huiping Wang, PhD;, Christopher S. Wilcox, MD, PhD; Michael Hultström, MD, PhD; Xiaofu Zhou, PhD;, En Yin Lai, MD, PhD  Circ Heart Fail. 2016; 9(--):1-10. >>> AntagomiR-7b; RNA, small interfering GABBR1; angiotensin II; CSF/CNS (paraventricular nucleus); Rat; 1004; 1002; animal info (male, Wistar, 180-200g); pumps replaced after 4 weeks; bilateral cannula used; tissue perfusion (paraventricular nucleus); cardiovascular; peptides; bilateral infusion; Dose (AntagomiR-7b or Ad-siGABBR1 40 ng/h; antiotensin II 1 ng/kg/min);.

15731 Robert W. Regenhardt,, Adam P. Mecca,, Fiona Desland,, Phillip F. Ritucci-Chinni,, Jacob A. Ludin,, David Greenstein,, Cristina Banuelos,, Jennifer L. Bizon,, Mary K. Reinhard, and Colin Sumners  Experimental Physiology 2014; 99(-2-):442-453. >>> Angiotensin (1-7); A779; aCSF; CSF/CNS (left lateral cerebral ventricle); rat; 2006; Controls received mp w/ vehicle; animal info; Male spSHRs, treated until 91 days old (~248 g); ALZET brain infusion kit 1 used; ischemia (ischaemic stroke in the brain); behavioral testing (sunflower seed-eating task to determine fine motor function and neurological deficits); long-term study 6 weeks; peptides delivered; A779 aka (D-Ala7)-Ang-(1–7); Dose: Ang-(1–7) (1.0Ǩg in 0.15Ǩl ACSF h−1);(D-Ala7)-Ang-(1–7) (A779; 2.5Ǩg in 0.15Ǩl ACSF h−1).

15248 Zhu, D.L., Tong, Q., Liu, W., Tian, M.J., Xie, W., Ji, L., Shi, J.P. Angiotensin (1-7) protects against stress-induced gastric lesions in rats. BIOCHEMICAL PHARMACOLOGY 2014; 87(-3-):467-476. >>> Angiotensin (1-7); A779; CSF, artificial; CSF/CNS; Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 280-310g); functionality of mp verified by plasma levels; ALZET brain infusion kit used; no stress"No stress was induced by this surgical procedure, because there are no significant alterations in body weight and motor activity in our preliminary study"(see pg. 468); cardiovascular; peptides;.

15163 Yu, Y., Xue, B.J., Wei, S.G., Zhang, Z.H., Beltz, T.G., Guo, F., Johnson, A.K., Felder, R.B., Felder, R.B. Activation of Central PPAR-gamma Attenuates Angiotensin II-Induced Hypertension. HYPERTENSION 2015; 66(--):403-411. >>> Angiotensin II; pioglitazone, GW9662; DMSO; CSF, artificial; SC; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-300g); functionality of mp verified by residual volume; 20% DMSO used; Multiple pumps per animal (2); cardiovascular; immunology; peptides; Cannula placement verified via histologic analysis of cannula track; bp measured using radiotelemetry (DSI);.

15053 Nieto, F.R., Clark, A.K., Grist, J., Chapman, V., Malcangio, M., Malcangio, M. Calcitonin Gene-Related Peptide-Expressing Sensory Neurons and Spinal Microglial Reactivity Contribute to Pain States in Collagen-Induced Arthritis. ARTHRITIS&RHEUMATOLOGY 2015; 67(--):1668-1677. >>> Calcitonin gene-related peptide (8-37); CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ vehicle; animal info (female, Lewis, 180-200g); behavioral testing (Von Frey filaments; Plantar test); immunology; peptides;.

14989 Jo, F., Jo, H., Hilzendeger, A.M., Thompson, A.P., Cassell, M.D., Rutkowski, D.T., Davisson, R.L., Grobe, J.L., Sigmund, C.D., Sigmund, C.D. Brain Endoplasmic Reticulum Stress Mechanistically Distinguishes the Saline-Intake and Hypertensive Response to Deoxycorticosterone Acetate-Salt. HYPERTENSION 2015; 65(--):1341-U330. >>> Tauroursodeoxycholic acid; angiotensin II; CSF, artifical; CSF/CNS; SC; Mice; Controls received mp w/ vehicle; animal info (CHOP-deficient or C57BL6J, 6 weeks old); cardiovascular; peptides; Tauroursodeoxycholic acid aka TUDCA; used Plastics One cannula;.

14918 Dewji, N.N., Singer, S.J., Masliah, E., Rockenstein, E., Kim, M., Harber, M., Horwood, T., Dewji, N.N. Peptides of Presenilin-1 Bind the Amyloid Precursor Protein Ectodomain and Offer a Novel and Specific Therapeutic Approach to Reduce beta-Amyloid in Alzheimer's Disease. PLoS One 2015; 10(--):U263-U284. >>> P4; P8; PBS; CSF/CNS; Mice (transgenic); 1002; Controls received mp w/ vehicle; animal info (mThy1-hAPP Tg, 6 months old); ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); brain tissue distribution; peptides;.

14837 Bao, Y.J., Gao, Y.B., Hou, W., Yang, L.P., Kong, X.Y., Zheng, H.G., Li, C.H., Hua, B.J., Hua, B.J. Engagement of signaling pathways of protease-activated receptor 2 and -opioid receptor in bone cancer pain and morphine tolerance. INTERNATIONAL JOURNAL OF CANCER 2015; 137(--):1475-1483. >>> FSLLRY-NH2; Saline; CSF/CNS (intrathecal); Rat; Controls received mp w/ vehicle; animal info (Wistar 200-250g); cancer (bone); no stress (see pg. 1476); behavioral testing (mechanical paw withdrawal, thermal hyperalgesia); peptides;.

14493 Xue, B.J., Zhang, Z.M., Beltz, T.G., Guo, F., Hay, M., Johnson, A.K., Xue, B.J. Estrogen regulation of the brain renin-angiotensin system in protection against angiotensin II-induced sensitization of hypertension. American Journal of Physiology-Heart and Circulatory Physiology 2014; 307(--):H191-H198. >>> Angiotensin II; estradiol, 17b-; angiotensin (1-7); A-779; Saline; DMSO; SC; CSF/CNS; Rat; 2001; 2002; Animal info (Sprague Dawley, 10-12 weeks old); ALZET brain infusion kit used; Multiple pumps per animal (2); replacement therapy (ovarectomized, estradiol infusion); cardiovascular; antihypertensive; peptides; bp measured using radiotelemetry (DSI);.

14476 Wei, W., Motoike, T., Krzeszinski, J.Y., Jin, Z.X., Xie, X.J., Dechow, P.C., Yanagisawa, M., Wan, Y.H., Wan, Y.H. Orexin Regulates Bone Remodeling via a Dominant Positive Central Action and a Subordinate Negative Peripheral Action. Cell Metabolism 2014; 19(--):927-940. >>> [Ala11, D-Leu15] Orexin-B;; CSF/CNS; Mice; 1004; Controls received mp w/ PBS; animal info (OX-KO); peptides; used Plastics one cannula (3300PM/SPC); Orexin-B is an OX2R selective agonist; osteoporosis;.

14440 Thakker-Varia, S., Behnke, J., Doobin, D., Dalal, V., Thakkar, K., Khadim, F., Wilson, E., Palmieri, A., Antila, H., Rantamaki, T., Alder, J., Alder, J. VGF (TLQP-62)-induced neurogenesis targets early phase neural progenitor cells in the adult hippocampus and requires glutamate and BDNF signaling. Stem Cell Research 2014; 12(--):762-777. >>> TLQP-62; CSF/CNS; Mice (transgenic); 2004; Controls received mp w/ saline; animal info (male, Nestin-GFP transgenic, 3-4 months old, 30g); ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; Cannula placement verified via histologic analysis;.

14393 Secher, A., Jelsing, J., Baquero, A.F., Hecksher-Sorensen, J., Cowley, M.A., Dalboge, L.S., Hansen, G., Grove, K.L., Pyke, C., Raun, K., Schaeffer, L., Tang-Christensen, M., Verma, S., Witgen, B.M., Vrang, N., Knudsen, L.B., Knudsen, L.B. The arcuate nucleus mediates GLP-1 receptor agonist liraglutide-dependent weight loss. Journal of Clinical Investigation 2014; 124(--):4473-4488. >>> Exendin (9-39); DPBS buffer; PBS; CSF/CNS; SC; Rat; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 190g); Multiple pumps per animal (2, one ICV one SC); post op. care (QD Carprofen injection); peptides; used anchoring screws; pumps primed overnight at 37C saline; obesity;.

14318 Neal-Perry, G., Yao, D.C., Shu, J., Sun, Y., Etgen, A.M., Neal-Perry, G. Insulin-Like Growth Factor-I Regulates LH Release by Modulation of Kisspeptin and NMDA-Mediated Neurotransmission in Young and Middle-Aged Female Rats. Endocrinology 2014; 155(--):1827-1837. >>> JB-1; insulin-like growth factor-1;; CSF, artificial; CSF/CNS (third ventricle); Rat; 2002; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 3-4 months or 9-11 months old, ovariectomized); peptides; cannula placement verified via dye infusion; used Plastics One cannula; JB-1 is a selective antagonist of IGF-Ir;.

14710 Deshotels, M.R., Xia, H.J., Sriramula, S., Lazartigues, E., Filipeanu, C.M., Lazartigues, E. Angiotensin II Mediates Angiotensin Converting Enzyme Type 2 Internalization and Degradation Through an Angiotensin II Type I Receptor-Dependent Mechanism. Hypertension 2014; 64(--):1368-U438. >>> Angiotensin II; leupeptin; Saline; cerebrospinal fluid, artificial; SC; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (C57Bl6 or ACE2 KO, 25-30g, 14-16 weeks old); cardiovascular; peptides; bp measured using radiotelemetry; Ang II infused SC; leupeptin infused ICV;.

14660 An, K., Jung, J.H., Jeong, A.Y., Kim, H.G., Jung, S.Y., Lee, K., Kim, H.J., Kim, S.J., Jeong, T.Y., Son, Y., Kim, H.S., Kim, J.H., Kim, J.H. Neuritin can normalize neural deficits of Alzheimer's disease. Cell Death&Disease 2014; 5(--):U181-U190. >>> Neuritin; CSF, artificial; CSF/CNS; Mice (transgenic); 1007D; Controls received mp w/ vehicle; animal info (Tg2576, 6 months old); ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); peptides; pumps primed in 37C saline;.

14591 Trovo, L., Stroobants, S., D'Hooge, R., Ledesma, M.D., Dotti, C.G., Trovo, L. Improvement of biochemical and behavioral defects in the Niemann-Pick type A mouse by intraventricular infusion of MARCKS. NEUROBIOLOGY OF DISEASE 2015; 73(--):319-326. >>> MARCKS peptide; Saline, sterile; CSF/CNS; Mice; 1004; Control animals received mp w/ vehicle; animal info (ASMko, 4 mo old); peptides; ALZET brain infusion kit 3 used; cyanoacrylate used; behavioral testing (accelerating rotarod); MARCKS is a protein required for PI(4,5)P2 membrane clustering and hydrolysis.

13953 Xue, B.J., Zhang, Z.M., Johnson, R.F., Guo, F., Hay, M., Johnson, A.K. Central endogenous angiotensin-(1-7) protects against aldosterone/NaCl-induced hypertension in female rats. American Journal of Physiology-Heart and Circulatory Physiology 2013; 305(-5-):H699-H705. >>> Angiotensin (1-7), D-alanine; A-779; CSF/CNS; SC; Rat; 2004; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 10-12 weeks old, OVX); ALZET brain infusion kit used; replacement therapy (ovariectomy); cardiovascular; peptides; antihypertensive; A-779 is an angiotensin (1-7) receptor antagonist; bp measured using radiotelemetry;.

13828 Kang, Y.M., Zhang, D.M., Yu, X.J., Yang, Q., Qi, J., Su, Q., Suo, Y.P., Yue, L.Y., Zhu, G.Q., Qin, D.N. Chronic infusion of enalaprilat into hypothalamic paraventricular nucleus attenuates angiotensin II-induced hypertension and cardiac hypertrophy by restoring neurotransmitters and cytokines. TOXICOLOGY AND APPLIED PHARMACOLOGY 2014; 274(-3-):436-444. >>> Angiotensin II; enalaprilat; Saline; cerebrospinal fluid, artificial; SC; CSF/CNS (paraventricular nucleus); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 275-300g); functionality of mp verified by increased bp; post op. care (buprenorphine); tissue perfusion (bilateral paraventricular nucleus); cardiovascular; peptides; antihypertensive; bp measured using tail-cuff; pumps primed in 37C saline; used wound clips; enalaprilat is an ACE inhibitor; Plastics One bilateral PVN cannulae; bilaterial infusion.

13793 Guimaraes, P.S., Oliveira, M.F., Braga, J.F., Nadu, A.P., Schreihofer, A., Santos, R.A.S., Campagnole-Santos, M.J., Campagnole-Santos, M.J. Increasing Angiotensin-(1-7) Levels in the Brain Attenuates Metabolic Syndrome-Related Risks in Fructose-Fed Rats. Hypertension 2014; 63(--):1078-1085. >>> Angiotensin (1-7); CSF/CNS; Rat; 2004; Controls received mp w/ saline; animal info (Sprague Dawley); cardiovascular; antihypertensive; peptides; diabetes; bp measured using catheter;.

13752 Dange, R.B., Agarwal, D., Masson, G.S., Vila, J., Wilson, B., Nair, A., Francis, J., Francis, J. Central blockade of TLR4 improves cardiac function and attenuates myocardial inflammation in angiotensin II-induced hypertension. Cardiovascular Research 2014; 103(--):17-27. >>> Angiotensin II; peptide, viral inhibitory; SC; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle or control peptide; animal info (male, Sprague Dawley, 10 weeks old, 250-350g); functionality of mp verified by plasma levels ELISA; Multiple pumps per animal (2); cardiovascular; peptides; viral inhibitory peptide aka VIPER; VIPER inhibits TLR4; bp measured using radiotelemetry;.

13726 Calcagnoli, F., Meyer, N., de Boer, S.F., Althaus, M., Koolhaas, J.M., Calcagnoli, F. Chronic enhancement of brain oxytocin levels causes enduring anti-aggressive and pro-social explorative behavioral effects in male rats. Hormones and Behavior 2014; 65(--):427-433. >>> Oxytocin, synthetic; antagonist, peptidergic oxytocin; Saline, sterile, pyrogen-free; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Groningen, adult); ALZET brain infusion kit 2 used; behavioral testing (resident intruder test); stability verified by (pilot study; 44% destruction of OVT over 10 day period in vivo); peptides; Cannula placement verified via dye injection after sacrifice; Plastics One guide cannula; dental acrylic cement and two stainless steel screws; pumps and catheters incubated in room temperature saline overnight;.

13710 Avolio, E., Mahata, S.K., Mantuano, E., Mele, M., Alo, R., Facciolo, R.M., Talani, G., Canonaco, M., Avolio, E. ANTIHYPERTENSIVE AND NEUROPROTECTIVE EFFECTS OF CATESTATIN IN SPONTANEOUSLY HYPERTENSIVE RATS: INTERACTION WITH GABAERGIC TRANSMISSION IN AMYGDALA AND BRAINSTEM. Neuroscience 2014; 270(--):48-57. >>> Catestatin; muscimol; Saline; PBS; CSF/CNS (amygdala, central nucleus); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Spontaneously Hypertensive Rat, 3, 6, or 9 months old); ALZET brain infusion kit 2 used; tissue perfusion (central nucleus of amygdala); cardiovascular; antihypertensive; peptides; Cannula placement verified via histological analysis and methylene blue injection; Catestatin aka CST; muscimol aka MUS; used acrylic dental cement; bp measured using using radiotelemetry; CST is a catecholamine release inhibitor; muscimol is a GABAa receptor agonist;.

13704 de Araujo, C.B., Russo, L.C., Castro, L.M., Forti, F.L., do Monte, E.R., Rioli, V., Gozzo, F.C., Colquhoun, A., Ferro, E.S., Ferro, E.S. A Novel Intracellular Peptide Derived from G(1)/S Cyclin D2 Induces Cell Death. Journal of Biological Chemistry 2014; 289(--):16711-16726. >>> Pep5-cpp; N-pep5-cpp; Cerebrospinal fluid, artificial; CSF/CNS; Rat; 2002; Animal info (male, Wistar, adult, 250-350g ); ALZET brain infusion kit used; cancer (glioma); peptides;.

13691 Xie, W., Zhu, D.L., Ji, L., Tian, M.J., Xu, C., Shi, J.P., Shi, J.P. Angiotensin-(1-7) improves cognitive function in rats with chronic cerebral hypoperfusion. Brain Research 2014; 1573(--):44-53. >>> Angiotensin (1-7); A779; CSF/CNS; Rat; 2004; Control animals received mp w/ aCSF; animal info (male, Wistar, 300-350 g); ALZET brain infusion kit 2 used; aCSF recipe; peptides.

13684 Alkadhi, K.A., Tran, T.T., Alkadhi, K.A. Chronic psychosocial stress impairs early LTP but not late LTP in the dentate gyrus of at-risk rat model of Alzheimer's disease. Brain Research 2014; 1588(--):150-158. >>> Amyloid protein, beta (1-42); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; Animal info (Wistar, 200-225 g); peptides; 14 day pump used; neurodegenerative (Alzheimer's disease).

13656 Park, A.J., Collins, J., Blennerhassett, P.A., Ghia, J.E., Verdu, E.F., Bercik, P., Collins, S.M. Altered colonic function and microbiota profile in a mouse model of chronic depression. NEUROGASTROENTEROLOGY AND MOTILITY 2013; 25(-9-):733-E575. >>> Corticotropin releasing hormone; Saline; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (female, C57BL/6, 8-10 weeks old); behavioral testing (Step down test, tail suspension test, open field); peptides; cyanoacrylate adhesive; Corticotropin-rel. factor aka Corticotropin releasing hormone; Plastics 1 cannula, DURECT PE 60 tubing..

13654 Ortega, F., Gascon, S., Masserdotti, G., Deshpande, A., Simon, C., Fischer, J., Dimou, L., Lie, D.C.C., Schroeder, T., Berninger, B. Oligodendrogliogenic and neurogenic adult subependymal zone neural stem cells constitute distinct lineages and exhibit differential responsiveness to Wnt signalling. NATURE CELL BIOLOGY 2013; 15(-6-):602-U372. >>> Wnt3a; Cerebrospinal fluid, artificial; CSF/CNS; Mice; 2001; Controls received mp w/ vehicle; animal info ( 2-3 months old, C57B2/6J, GLAST, hGFAP-RFP, SOX10, CAG CAT GFP); ALZET brain infusion kit 2 used; peptides Wnt3a;.

13652 Nelson, B.S., Springer, R.C., Daniel, J.M. Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats. Psychopharmacology 2014; 231(-5-):899-907. >>> JB1; Cerebrospinal fluid, artificial; CSF/CNS; Rat; 2006; Controls received mp w/ vehicle; animal info (female, Long Evans, 2 months old); ALZET brain infusion kit used; stress/adverse reaction: (see pg. 901); behavioral testing (radial maze); replacement therapy (ovarectomized); peptides; Cannula placement verified via Cresyl violet staining; cannula anchored to skull with screws and dental acrylic; JB1 is a IGF-1 receptor antagonist.

13644 Khan, Z., Combadiere, C., Authier, F.J., Itier, V., Lux, F., Exley, C., Mahrouf-Yorgov, M., Decrouy, X., Moretto, P., Tillement, O., Gherardi, R.K., Cadusseau, J. Slow CCL2-dependent translocation of biopersistent particles from muscle to brain. BMC Medicine 2013; 11(-;-):U106-U123. >>> CCL2, rodent; CSF/CNS; Mice; 1002; Controls received mp w/ phosphate buffered saline (PBS); animal info (mice: B57/B6, 8-10 week C57BL/D, mdx, CX3CRI GFP/+ and CCL2); peptides; cytokine; CCL2 aka chemokine ligand 2.

13642 Howell, K.K., Monk, B.R., Carmack, S.A., Mrowczynski, O.D., Clark, R.E., Anagnostaras, S.G. Inhibition of PKC disrupts addiction-related memory. Frontiers in Behavioral Neuroscience 2014; 8(-;-):U1-U9. >>> Peptide, zeta-inhibitory; chelerythrine; Cerebrospinal fluid, artificial; PBS; CSF/CNS (third ventricle); Mice; 1002; Controls received mp w/ vehicle; animal info (C57BL/6Jx129T2SvEms/J, adult); ALZET brain infusion kit 3 used; post op. care (buprenorphine injection); behavioral testing (locomotion, locomotor sensitization); pulsatile delivery; dependence; peptides; zeta-inhibitory peptide aka ZIP; Lynch coil of ZIP administration for 14.5 hours; used mineral oil; Schematic of pump implantation pg.3 Fig 1A;.

13559 O'Carroll, S.J., Gorrie, C.A., Velamoor, S., Green, C.R., Nicholson, L.F.B. Connexin43 mimetic peptide is neuroprotective and improves function following spinal cord injury. NEUROSCIENCE RESEARCH 2013; 75(-3-):256-267. >>> Peptide5, mimetic; Cerebrospinal fluid, artificial; CSF/CNS (intrathecal); Rat; 2001D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley); spinal cord injury; post op. care (Cephalothin sodium 10 mg twice a day until urine cleared, Carpofen 0.5 mg twice a day for 3 days, Hartman's replacement solution 3 ml twice a day for 3 days, Bladders expressed twice a day for 1-2 weeks); behavioral testing (Open field test); peptides;.

13476 Rodriguez-Pacheco, F., Novelle, M.G., Vazquez, M.J., Garcia-Escobar, E., Soriguer, F., Rojo-Martinez, G., Garcia-Fuentes, E., Malagon, M.M., Dieguez, C. Resistin Regulates Pituitary Lipid Metabolism and Inflammation In Vivo and In Vitro. Mediators of Inflammation 2013; ;(-;-):U1-U8. >>> Resistin; Saline; CSF/CNS; Rat; 2001D, 2ML2; Controls received mp w/ vehicle; animal info (Sprague Dawley, male, 300-350g); ALZET brain infusion kit used; post op. care (individually caged); immunology; peptides; Cannula placement verified via methylene blue staning; Resistin aka ADSF or C/EBP;.

13471 Pereira, P.A., Santos, D., Neves, J., Madeira, M.D., Paula-Barbosa, M.M. Nerve growth factor retrieves neuropeptide Y and cholinergic immunoreactivity in the nucleus accumbens of old rats. NEUROBIOLOGY OF AGING 2013; 34(-8-):1988-1995. >>> Nerve growth factor; Cerebrospinal fluid, artificial; bovine serum albumin; CSF/CNS; SC; Rat; 2002; Animal info (male, Wistar, 6-24 months old); functionality of BIK verified by methylene blue staining; ALZET brain infusion kit used; 0.1% Bovine Serum Albumin used; neurodegenerative (aging); post op. care (SC injections 2ml of 0.9% saline); pulsatile delivery (used coiled Intramedic PE-60; 0.1% methylene blue with air-oil spacer at pump end with agent); peptides;.

13470 Piekarski, D.J., Zhao, S., Jennings, K.J., Iwasa, T., Legan, S.J., Mikkelsen, J.D., Tsutsui, K., Kriegsfeld, L.J. Gonadotropin-inhibitory hormone reduces sexual motivation but not lordosis behavior in female Syrian hamsters (Mesocricetus auratus). Hormones and Behavior 2013; 64(-3-):501-510. >>> Gonadotropin-inhibitory hormone; Saline; CSF/CNS; Hamster; 2002; Controls received mp w/ vehicle; animal info (female, Syrian hamsters, adult); functionality of mp verified pg503; behavioral testing (partner preferences, vaginal scent marking, lordosis test); peptides; Cannula placement verified via angiotensin injection; GnIH aka RFamide related peptide-3; pumps primed overnight.

13460 Sakai, A., Suzuki, H. Nerve injury-induced upregulation of miR-21 in the primary sensory neurons contributes to neuropathic pain in rats. Biochemical and Biophysical Research Communications 2013; 435(-2-):176-181. >>> Antagomir, miR-21, inhibitor; interleukin, beta; Saline; CSF/CNS (intrathecal); Rat; Controls received mp w/ negative control miR-21 inhibitor (for miR-21) or vehicle (for IL-beta); animal info (male, Sprague Dawley, 5 weeks old); behavioral testing (Paw withdrawal); peptides;.

13454 Lee, Y.S., Danandeh, A., Baratta, J., Lin, C.Y., Yu, J., Robertson, R.T. Neurotrophic factors rescue basal forebrain cholinergic neurons and improve performance on a spatial learning test. Experimental Neurology 2013; 249(-;-):178-186. >>> Nerve growth factor; neurotrophin-3; PBS; CSF/CNS; Rat; 2004; Controls received mp w/ saline; animal info (Sprague Dawley, 8 weeks old, 180g); bilateral Y-connector used; post op. care (restricted diet); behavioral testing (Delayed match to position T maze); peptides; bilateral infusion;.

13452 Shaqura, M., Khalefa, B.I., Shakibaei, M., Winkler, J., Al-Khrasani, M., Fuerst, S., Mousa, S.A., Schaefer, M. Reduced Number, G Protein Coupling, and Antinociceptive Efficacy of Spinal Mu-Opioid Receptors in Diabetic Rats Are Reversed by Nerve Growth Factor. JOURNAL OF PAIN 2013; 14(-7-):720-730. >>> Nerve growth factor; Cerebrospinal fluid, artificial; rat serum albumin; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Wistar, 225g); behavioral testing (paw pressure test); peptides; used PE-10 attached to PE-60 catheter.

13449 Song, D.Y., Yu, H.N., Park, C.R., Lee, J.S., Lee, J.Y., Park, B.G., Woo, R.S., Han, J.T., Cho, B.P., Baik, T.K. Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss. BMC NEUROSCIENCE 2013; 14(-;-):U1-U13. >>> Tuftsin fragment 1-3; Cerebrospinal fluid, artificial; CSF/CNS; Rat; 2002; 2004; Animal info (male, Wistar, adult, 250-300g); ALZET brain infusion kit 3 used; neurodegenerative (Parkinson's disease); immunology; peptides; Tuftsin fragment 1-3 is a human IgG derived tripeptide.

13448 Sriramula, S., Cardinale, J.P., Francis, J. Inhibition of TNF in the Brain Reverses Alterations in RAS Components and Attenuates Angiotensin II-Induced Hypertension. PLoS One 2013; 8(-5-):U584-U592. >>> Angiotensin II; Etanercept; Saline; cerebrospinal fluid, artificial; CSF/CNS; SC; Rat; 1004; 2004; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 10-12 weeks old); functionality of mp verified by increased BP; Multiple pumps per animal (2 pumps - one with Angiotensin, other with entanercept); post op. care (benzathine penicillin (30000 U, I.M.), buprenorphine (0.1mg/kg, S.C.) after surgery and 12 hours postop); cardiovascular; antihypertensive; peptides; bp measured using radiotelemetry (DSI);.

13425 Yan, J.G., Zhang, L.L., Agresti, M., LoGiudice, J., Yan, Y.H., Wang, Z.Y., Sanger, J.R., Matloub, H.S. The effect of calcium modulating agents on peripheral nerve recovery after crush. Journal of Neuroscience Methods 2013; 217(-1-2-):54-62. >>> Nifedipine; calcitonin; CSF/CNS (sciatic nerve); Rat; 2006; Controls received mp w/ saline or sham only; animal info (3 month old, male, Sprague-Dawley 250-300g); functionality of mp verified by decrease in calcium levels; peptides; Picture of MP p56, Fig1A. MP Pump setup p56, Fig1B;.

13421 Yang, H.J., Hwang, J.T., Kwon, D.Y., Kim, M.J., Kang, S., Moon, N.R., Park, S. Yuzu Extract Prevents Cognitive Decline and Impaired Glucose Homeostasis in beta-Amyloid-Infused Rats. Journal of Nutrition 2013; 143(-7-):1093-1099. >>> Amyloid protein, beta (25-35); amyloid protein, beta (35-25); CSF/CNS (bilateral CA1 region); Rat; Animal info (male, Sprague-Dawley, 232g); behavioral testing (Morris Water Maze, Passive avoidance test, locomotive activity); peptides;.

13417 Mantilla, C.B., Gransee, H.M., Zhan, W.Z., Sieck, G.C. Motoneuron BDNF/TrkB signaling enhances functional recovery after cervical spinal cord injury. Experimental Neurology 2013; 247(-;-):101-109. >>> Brain-derived neurotrophic factor;; CSF/CNS (intrathecal); Rat; 2002; Animal info (male, Sprague Dawley, adult); spinal cord injury; post op. care (Acetominophen orally and buprenorphine IM for 3 days); pulsed delivery (delayed delivery for 3 days - aCSF only in catheter); tissue perfusion (C4 segment of spine); peptides;"Mini-osmotic pumps were successfully implanted for intrathecal delivery at the C4 level and functioned properly throughout the 14 day duration of the experiment."pg 103"Differences in survival rates across groups were likely unrelated to the possible additional morbidity associated with intrathecal catheter and miniosmotic pump implantation, as the survival rate was 70% (28 out of 40) compared to 84% (26 out of 31) in rats not implanted with an intrathecal pump (p = 0.17)."pg 103; PE-10 intrathecal cannula, 10 cm.

13395 Kiyomoto, M., Shinoda, M., Okada-Ogawa, A., Noma, N., Shibuta, K., Tsuboi, Y., Sessle, B.J., Imamura, Y., Iwata, K. Fractalkine Signaling in Microglia Contributes to Ectopic Orofacial Pain following Trapezius Muscle Inflammation. Journal of Neuroscience 2013; 33(-18-):7667-7680. >>> Antibody, anti CX3CRI; Fractalkine; Antibody, anti interleukin-beta; Saline; CSF/CNS (cisterna magna); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 210-260g); post op. care (benzyl penicillin potassium, 20000U); behavioral testing (Rota-rod performance, Water tap reaching performance); immunology; peptides.

13372 Freeman, J.N., do Carmo, J.M., Adi, A.H., da Silva, A.A. Chronic central ghrelin infusion reduces blood pressure and heart rate despite increasing appetite and promoting weight gain in normotensive and hypertensive rats. Peptides 2013; 42(-;-):35-42. >>> Ghrelin; Saline; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle; animal info (male, Sprague Dawley&Spontaneous Hypertensive Rats, 275-330 g); comparison of IV infusion vs mp"the same dose of ghrelin was administered intravenously for 7 days to test if any potential spillover of ghrelin into the systemic circulation could account for its hypotensive effects, however no significant change in HR, BP or food intake was observed during the 7-day treatment period"(see pg. 39-40, Fig 3); stress/adverse reaction: (see pg. 39); behavioral testing (air-jet stress test); cardiovascular; peptides; Cannula placement verified via dipsogenic response to ICV injection of 100 ng Angiotensin II; used tygon tubing for catheter.

13335 Sardi, C., Zambusi, L., Finardi, A., Ruffini, F., Tolun, A.A., Dickerson, I.M., Righi, M., Zacchetti, D., Grohovaz, F., Provini, L., Furlan, R., Morara, S. Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis. Journal of Neuroimmunology 2014; 271(--):18-29. >>> Calcitonin gene-related peptide; CSF/CNS (intrathecal); Mice; 2002; Controls received mp w/ artificial CSF; animal info (alpha CGRP deficient 129S6); peptides.

13317 Gariepy, H.D., Talbot, S., Senecal, J., Couture, R. Brain kinin B(1) receptor contributes to the onset of stereotypic nocifensive behavior in rat. Behavioural Brain Research 2013; 241(-;-):17-26. >>> Angiotensin II; CSF/CNS; Rat; 2002; Animal info (WKY); guide cannula used; peptides.

13277 Shim, J.W., Sandlund, J., Han, C.H., Hameed, M.Q., Connors, S., Klagsbrun, M., Madsen, J.R., Irwin, N. VEGF, which is elevated in the CSF of patients with hydrocephalus, causes ventriculomegaly and ependymal changes in rats. Experimental Neurology 2013; 247(-;-):703-709. >>> Vascular endothelial growth factor-A165; Bevacizumab; Saline; BSA; CSF/CNS; Rat; 2001D; Controls received mp w/vehicle, or (saline, BSA); Peptides; animal info (male Sprague-Dawley rats, 250g); Brain infusion kit (2) used;.

13137 Chao, Y.M., Lai, M.D., Chan, J.Y.H. Redox-Sensitive Endoplasmic Reticulum Stress and Autophagy at Rostral Ventrolateral Medulla Contribute to Hypertension in Spontaneously Hypertensive Rats. Hypertension 2013; 61(-6-):1270-U302. >>> Angiotensin II; salubrinal; phosphatase inhibitor cocktail; CSF, aritificial; DMSO; CSF/CNS (cisterna magna); Rat; 1007D; Control animals received mp w/ vehicle; animal info (Wistar-Kyoto, SHR);"Drainage of cerebrospinal fluid (CSF) from the outer end of catheter ensured patency of the implantation."expanded methods; 2% DMSO used; CSF recipe; peptides; bp measured using radiotelemetry;.

13072 Tsai, C.Y., Su, C.H., Baudrie, V., Laude, D., Weng, J.C., Chang, A.Y.W., Chan, J.Y.H., Elghozi, J.L., Chan, S.H.H. Visualizing oxidative stress-induced depression of cardiac vagal baroreflex by MRI/DTI in a mouse neurogenic hypertension model. NEUROIMAGE 2013; 82(-;-):190-199. >>> Angiotensin II; CSF, artificial; CSF/CNS; Mice; 2001; Control animals received mp w/ vehicle; animal info (C57BL/6, male, adult, 26 g); peptides; bp measured using radiotelemetry (DSI);.

 

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What researchers are saying...

“We administered nicotine to achieve a steady-state concentration, using subcutaneously implanted minipumps, a technique that avoids the episodic hypoxic-ischemic episodes that accompany nicotine injections, and that also sidesteps the inherent problems of repeated handling stress and episodic withdrawal between injections” Slotkin et al., Neurotoxicology and Teratology 2002; 24:369-384.