References on the Administration of Erythropoietin Using ALZET® Osmotic Pumps

15817 Eduardo H. Sanchez-Mendoza, Jeismar Carballo, Marines Longart, Dirk M. Hermann, Thorsten R. Doeppner  Journal of Visualized Experiments 2016; 107(--):1-9. >>> Erythropoietin, recombinant human; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (C57BL6); good methods (Jove Video; picture of pump and implantation pg. 4); ischemia (cerebral); post op. care (Carprofen 4 mg/kg); behavioral testing (rotarod test; hand grip strength); cyanoacrylate adhesive;“In this work we have shown the method of implantation of minipumps with a cannula connected to the skull in order to deliver the plasticity promoting protein rhEpo directly into the ventricle, thus circumventing the BBB.” pg 8; Cannula placement verified via histologic analysis"The are no evident severe tissue alterations based on Nissl staining as compared to the corresponding contralateral area";.

14965 Hiram-Bab, S., Liron, T., shet-Unger, N., Mittelman, M., Gassmann, M., Rauner, M., Franke, K., Wielockx, B., Neumann, D., Gabet, Y., Neumann, D. Erythropoietin directly stimulates osteoclast precursors and induces bone loss. FASEB JOURNAL 2015; 29(--):1890-1900. >>> Erythropoeitin; SC; Mice (transgenic); Controls received mp w/ vehicle; animal info (female, Tg6, 12 weeks old);.

13818 Jeffers, M.S., Hoyles, A., Morshead, C., Corbett, D., Corbett, D. Epidermal Growth Factor and Erythropoietin Infusion Accelerate Functional Recovery in Combination With Rehabilitation. Stroke 2014; 45(--):185-+. >>> Epidermal Growth Factor; erythropoietin; Cerebrospinal fluid, artificial; CSF/CNS; Rat; 2001; Animal info (male, Sprague Dawley); pumps replaced every 7 days; ischemia (cerebral); behavioral testing (staircase test); pumps removed 7 days after serial implantation;.

13569 Wang, Y.F., Cooke, M.J., Sachewsky, N., Morshead, C.M., Shoichet, M.S. Bioengineered sequential growth factor delivery stimulates brain tissue regeneration after stroke. JOURNAL OF CONTROLLED RELEASE 2013; 172(-1-):1-11. >>> Epidermal growth factor; erythropoietin; Cerebrospinal fluid, artificial; CSF/CNS; Mice; 1007D; Animal info (male, C57BL6, 9-11 weeks old); EGF-PGF pumps replaced after 7 days with pump filled with ETO; ALZET brain infusion kit 3 used; comparison of epicortical composite vs mp; stress/adverse reaction:"Unlike the ICV catheter/minipump,which causes significant tissue damage, the epicortical composite provides a minimal invasiveness and no tissue damage."(see pg.9); immunology; Pumps implanted 4 days after stroke; BIK implanted same day as stroke;.

13430 Wang, G.B., Ni, Y.L., Zhou, X.P., Zhang, W.F. The AKT/mTOR pathway mediates neuronal protective effects of erythropoietin in sepsis. MOLECULAR AND CELLULAR BIOCHEMISTRY 2014; 385(-1-2-):125-132. >>> Erythropoietin, human recombinant; PBS; BSA; SC; Rat; Controls received mp w/ vehicle or sham surgery; animal info (Sprague Dawley, 120 days old, 240-280g); behavioral testing (open field exploration, inhibitory avoidance, Morris water maze);.

12963 Yamaleyeva, L.M., Guimaraes-Souza, N.K., Krane, L.S., Agcaoili, S., Gyabaah, K., Atala, A., Aboushwareb, T., Yoo, J.J. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. STEM CELLS TRANSLATIONAL MEDICINE 2012; 1(-5-):373-383Erythropoietin. >>> Erythropoietin, recomb. human; Saline; IP; Rat (nude); 2ML4; Animal info (athymic, male, 10-15 wks old); pump functionality measured via residual volume.

12832 Unden, J., Sjoelund, C., Laensberg, J.K., Wieloch, T., Ruscher, K., Romner, B. Post-ischemic continuous infusion of erythropoeitin enhances recovery of lost memory function after global cerebral ischemia in the rat. BMC NEUROSCIENCE 2013; 14(-;-):U1-U8. >>> Erythropoietin; Saline; IV (jugular); Rat; 2001D; 1003D; Control animals received mp w/ vehicle; animal info (Wistar, male, 300-350 g); silastic tubing used.

11565 Reitmeir, R., Kilic, E., Kilic, U., Bacigaluppi, M., ElAli, A., Salani, G., Pluchino, S., Gassmann, M., Hermann, D.M. Post-acute delivery of erythropoietin induces stroke recovery by promoting perilesional tissue remodelling and contralesional pyramidal tract plasticity. Brain 2011; 134(-;-):84-99. >>> Erythropoietin; NaCl; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (C57Bl6/j, male, 23-25 g, 8-10 wks); ALZET brain infusion kit 3 used; ischemia (focal cerebral).

11557 Price, C.D., Yang, Z.J., Karlnoski, R., Kumar, D., Chaparro, R., Camporesi, E.M. Effect of continuous infusion of asialoerythropoietin on short-term changes in infarct volume, penumbra apoptosis and behaviour following middle cerebral artery occlusion in rats. Clinical and Experimental Pharmacology and Physiology 2010; 37(-2-):185-192. >>> Erythropoietin, asialo-; Saline, sterile; SC; Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-275 g).

11323 Erlandsson, A., Lin, C.H.A., Yu, F.G., Morshead, C.M. Immunosuppression promotes endogenous neural stem and progenitor cell migration and tissue regeneration after ischemic injury. Experimental Neurology 2011; 230(-1-):48-57. >>> Epidermal growth factor, recomb. human; erythropoietin; cyclosporine A; CSF/CNS; SC; Mice (NOD/SCID); 1007D; Animal info (male, C57/BL6, 8-10 wks old); pumps replaced after 7 days; ALZET brain infusion kit 3 used.

10826 Kondo, A., Shingo, T., Yasuhara, T., Kuramoto, S., Kameda, M., Kikuchi, Y., Matsui, T., Miyoshi, Y., Agari, T., Borlongan, C.V., Date, I. Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats. Brain Research 2009; 1296(-;-):127-136. >>> Erythropoietin, human, recomb.; antibody, anti-EPO; Saline; CSF/CNS; Rat; Controls received mp w/ rat serum albumin, or control mouse IgG; animal info (male, Fischer 344, 10-12 wks old, 200-250 g, SLC).

10542 Jobst, B.J., Riegger, G.A.J., Griese, D.P. Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model. Cardiovascular Drugs and Therapy 2009; 23(-5-):343-353. >>> Colony-stimulating factor, G, human, recomb.; erythropoietin, human, recomb.; IP; Rat; 2ML1; Controls received mp w/ saline; animal info (male, CD, 250 g, splenectomy).

10512 Jing, M., Shingo, T., Yasuhara, T., Kondo, A., Morimoto, T., Wang, F.F., Baba, T., Yuan, W.J., Tajiri, N., Uozumi, T., Murakami, M., Tanabe, M., Miyoshi, Y., Zhao, S.G., Date, I. The combined therapy of intrahippocampal transplantation of adult neural stem cells and intraventricular erythropoietin-infusion ameliorates spontaneous recurrent seizures by suppression of abnormal mossy fiber sprouting. Brain Research 2009; 1295(-;-):203-217. >>> Erythropoietin, human, recomb.; Saline; albumin, rat serum; CSF/CNS; Rat; 2001; Controls received mp w/ vehicle; animal info (male, 190-210 g).

10497 Kolb, B, Morshead, C., Gonzalez, C., Kim, M., Gregg, C., Shingo, T., Weiss, S. Growth factor-stimulated generation of new cortical tissue and functional recovery after stroke damage to the motor cortex of rats. Journal of Cerebral Blood Flow and Metabolism 2007; 27(--):983-997. >>> Erythropoietin; epidermal growth factor; CSF, artificial; CSF/CNS; Rat; 2001; Controls received mp w/ vehicle; peptides; animal info (male, Long-Evans, 90-110 days old); ischemia (cerebral); behavioral testing (forelimb assymetry, forelimb inhibition (swimming), reaching); some animals received 7 days EGF.

9738 Kadota, T., Shingo, T., Yasuhara, T., Tajiri, N., Kondo, A., Morimoto, T., Yuan, W.J., Wang, F.F., Baba, T., Tokunaga, K., Miyoshi, Y., Date, I. Continuous intraventricular infusion of erythropoietin exerts neuroprotective/rescue effects upon Parkinson's disease model of rats with enhanced neurogenesis. Brain Research 2009; 1254(-;-):120-127. >>> Erythropoietin; Albumin, rat serum; saline; CSF/CNS; Rat; 2001; Controls received mp w/ vehicle; animal info (Sprague Dawley, 220-250 g.).

9610 Belayev, L., Khoutorova, L., Zhao, K.L., Davidoff, A.W., Moore, A.F., Cramer, S.C. A novel neurotrophic therapeutic strategy for experimental stroke. Brain Research 2009; 1280(-;-):117-123. >>> Erythropoietin; Saline; IV (femoral); Rat; 2ML1; Controls received mp w/ vehicle; animal info (male, Long-Evans, 280-330 g., MCAO).

9284 Hack, M., Mascha, F.G., Jobst, B.J., Riegger, G.A.J., Griese, D.P. A systemic combination therapy with granulocyte-colony stimulating factor plus erythropoietin aggravates the healing process of balloon-injured rat carotid arteries. Cardiovascular Drugs and Therapy 2008; 22(-5-):351-362. >>> Colony-stimulating factor, G-, recomb. human; erythropoietin; IP; Rat; 2ML1; Controls received mp w/ saline; peptides; animal info (male, Sprague Dawley, 300-350 g., splenectomy);"We used implantable osmotic minipumps to guarantee continuous systemic delivery of the tested cytokines over the first even days upon intraperitoneal deposition."pg. 353.

9163 Oya, S., Yoshikawa, G., Takai, K., Tanaka, J.I., Higashiyama, S., Saito, N., Kirino, T., Kawahara, N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.

8889 Soliz, J., Gassmann, M., Joseph, V. Soluble erythropoietin receptor is present in the mouse brain and is required for the ventilatory acclimatization to hypoxia. JOURNAL OF PHYSIOLOGY-LONDON 2007; 583(-1-):329-336. >>> Erythropoietin receptor, soluble; Phosphate buffer; CSF/CNS; Mice; 1003D; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male C57/BL6, 3 months old, hypoxia).

8655 Frederiksen, B.L., Caye-Thomasen, P., Lund, S.P., Wagner, N., Asal, K., Olsen, N.V., Thomsen, J. Does erythropoietin augment noise induced hearing loss?. Hearing Research 2007; 223(-1-2-):129-137. >>> Erythropoietin; Ear (round window); Guinea-Pig; 1007D; Controls received mp w/ saline; replacement therapy (noise-induced hearing impairment); comparison of acute admin. vs. mp; peptides; animal info (male, Dunkin-Hartley); tissue perfusion (round window); mp primed 6 hours in 37 Celsius saline; correct catheter placement confirmed.

7822 Malhotra, S., Savitz, S.I., Ocava, L., Rosenbaum, D.M. Ischemic preconditioning is mediated by erythropoietin through PI-3 kinase signaling in an animal model of transient ischemic attack. Journal of Neuroscience Research 2006; 83(-1-):19-27. >>> Erythropoietin receptor, soluble; PBS; BSA; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; peptides; ischemia (cerebral); animal info (male, Wistar, 200-225 g); MCAO.

6108 Prass, K., Scharff, A., Ruscher, K., Loewl, D., Muselmann, C., Victorov, I., Kapinya, K., Dirnagl, U., Meisel, A. Hypoxia-induced stroke tolerance in the mouse is mediated by erythropoietin. Stroke 2003; 34(-8-):1981-1986. >>> Erythropoietin, soluble receptor; PBS; BSA; CSF/CNS; Mice; 1003D; Controls received mp w/ vehicle; peptides; ischemia (cerebral); human EPO used; 0.1%BSA used in vehicle.

5959 Shingo, T., Sorokan, S.T., Shimazaki, T., Weiss, S. Erythropoietin regulates the in vitro and in vivo production of neuronal progenitors by mammalian forebrain neural stem cells. J Neurosci 2001; 21(-24-):9733-9743. >>> Erythropoietin; antibody, rabbit anti-EPO neutralizing; rabbit IgG; Saline; albumin, mouse serum; rat serum; CSF/CNS; Mice; 1007D; Controls received mp w/ vehicle; peptides; Erythropoietin (EPO) Recomb. Human, antibody&IgG were dissolved in 0.9% saline with 1 mg/ml mouse serum albumin; pump/cannula schematic (p. 9739 fig A).

4723 Walker, B.R., Resta, T.C, Nelin, L.D  Am. J. Physiol. (Heart Circ. Physiol. ) 2000; 279(--):H2382-H2389. >>> Erythropoietin; Saline; rat; controls received mp w/vehicle; peptides; cardiovascular.

4391 Roeder, I., de Haan, G., Engel, C., Nijhof, W., Dontje, B., Loeffler, M. Interactions of erythropoietin, granulocyte colony-stimulating factor, stem cell factor, and interleukin-11 on murine hematopoiesis during simultaneous administration. Blood 1998; 91(-9-):3222-3229. >>> Interleukin-11; Stem cell factor; Colony-stimulating factor, G; Erythropoietin;; SC;; mice;; 2002; 1007D;; controls received mp w/ saline; functionality of mp verified by pilot studies; no stress (see pg. 3223); peptides; recomb. human interleukin-11, EPO,&G-CSF used; recomb. rat stem cell factor used (pegylated);agents were given in every combination;.

3075 Shibata, T., Kindler, V., Chicheportiche, Y., Vassalli, P., Izui, S. Interleukin 3 perfusion prevents death due to acute anemia induced by monoclonal antierythrocyte autoantibody. J. Exp. Med 1990; 171(--):1809-1814. >>> Interleukin-3; Colony-stimulating factor, GM; Erythropoietin; PBS; Glycerol; LPS, e. coli; SC; mice; 2002; controls received mp with vehicles +/- LPS; immunology; peptides; recomb. mouse GM-CSF&IL-3 used; recomb. human EPO used.

2829 Hoffman, A., Nyska, A., Avramoff, A., Golomb, G. Continuous versus pulsatile administration of erythropoietin (EPO) via the uterus in anemic rats. Int. J. Pharmaceutics 1994; 111(--):197-202. >>> Erythropoietin; intrauterine; rat; 2002; controls received intrauterine bolus of saline; replacement therapy (gentamicin-induced anemia); comparison of iv bolus and intrauterine bolus vs. mp; peptides; mp implanted subdermally in abdomen;"administration of drug at a constant rate produces considerably greater biological effect than that observed in the pulsatile mode."(pg. 200); tissue perfusion (uterus); recomb. human EPO used.

2634 de Haan, G., Engel, C., Dontje, B., Loeffler, M., Nijhof, W. Hemotoxicity by prolonged etoposide administration to mice can be prevented by simultaneous growth factor therapy. Cancer Res 1995; 55(--):324-329. >>> Erythropoietin; Colony-stimulating factor, G; Etoposide; SC; mice; dose-response curves (pg. 326-328); cancer; peptides; etoposide is VP-16-213; EPO and VP-16-213 given in same pump initially, but this inactived the G-CSF; multiple pumps per animal (1-2) for G-CSF/VP-16 mice.

2611 Berney, T., Shibata, T., Merino, R., Chicheportiche, Y., Kindler, V., Vassalli, P., Izui, S. Murine autoimmune hemolytic anemia resulting from Fc-gamma receptor-mediated erythrophagocytosis: protection by erythropoietin but not by interleukin-3, and aggravation by granulocyte-macrophage colony-stimulating factor. Blood 1992; 79(-11-):2960-2964. >>> Erythropoietin; Interleukin-3; Colony-stimulating factor, GM; PBS; Glycerol; SC; mice; 2002; immunology; peptides.

16 McGarry, M.P., Styles, B.D. Application of continuous flow osmotic minipumps in the study of hemopoietic regulators. Exp. Hematol 1978; 6(-10-):809-811. >>> Erythropoietin; SC; Mice; 1701; comparison of injections vs. mp infusion; peptides.


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What researchers are saying...

“However formoterol administered intraperitoneally twice a day did not influence LPS-induced premature delivery (data not shown). Osmotic Pumps can maintain the blood concentration of formoterol for over 20h. Thus, it may be of importance that formoterol was continuously and uniformly administered.” (p. 201) Shinkai et al., Reproduction 2003;125:199-203.