References on the Administration of Estrogens Using ALZET® Osmotic Pumps

16235 Rigassi, L., Barchiesi Bozzolo, F., Lucchinetti, E., Zaugg, M., Fingerle, J., Rosselli, M., Imthurn, B., Jackson, E. K., Dubey, R. K. 2-Methoxyestradiol blocks the RhoA/ROCK1 pathway in human aortic smooth muscle cells. Am J Physiol Endocrinol Metab 2015; 309(-12-):E995-1007. >>> Estradiol, 2-methoxy; DMSO; IA (carotid artery); rat; Controls received mp w/ vehicle; animal info (male Wistar Kyoto rats, 350 to 400 g); dose-response (E999); 0.1% DMSO; cardiovascular; cardiovascular; Balloon injury-induced neointima formation; Dose (350 ug/kg/day);.

16052 Tschiffely, A. E., Schuh, R. A., Prokai-Tatrai, K., Prokai, L., Ottinger, M. A. A comparative evaluation of treatments with 17beta-estradiol and its brain-selective prodrug in a double-transgenic mouse model of Alzheimer's disease. Horm Behav 2016; 83(--):39-44. >>> DHED; estradiol, 17B-; Propylene glycol; SC; Mice (transgenic); 2004; Controls received mp w/ vehicle; animal info (female, APPswe/PS1dE9, 6 months old); functionality of mp verified by plasma levels (see figure S1 - E2 only; no assay for DHED); pumps replaced every 4 weeks; neurodegenerative (Alzheimer’s); replacement therapy (estradiol infusion); DHED aka 10Ǟ,17Ǟ-dihydroxyestra-1,4-dien-3-one; Dose (2 ug/day); Industry authored (AgyPharma LLC);.

16050 Uchida, S., Kagitani, F. Sympathetic regulation of ovarian functions under chronic estradiol treatment in rats. Auton Neurosci 2016; 197(--):19-24. >>> Estradiol, 17B-; SC; Rat; 2004; Controls received mp w/ saline; animal info (female, Wistar, 5-6 months old); functionality of mp verified by estrogen-mediated phenomena (cutaneous infusion of 17Ǟ-estradiol (5Ǩg/day) for 4 weeks demonstrated the well-known estrogen-mediated phenomena of acyclic conditions and uterine hypertrophy… This indicates that estradiol was definitely administered to our study rats) pg 23; Dose (5 ug/day);.

16031 Kim, I. D., Ahn, K. H., Lee, S., Hong, S. C., Kim, S. H., Kim, T. Effect of ovariectomy, 17-beta estradiol, and progesterone on histology and estrogen receptors of bladder in female partial bladder outlet obstruction rat model. J Obstet Gynaecol Res 2013; 39(-7-):1259-67. >>> estrogen; progesterone; dehydroepiandrosterone; DMSO; SC; rat; animal info: Sprague-Dawley,bladder outlet obstruction model, ovariectomized model; functionality of mp verified by plasma measurement ; mp used to infuse estrogen, progesterone, and DHEA to examine their effect on angiogenesis of the bladder detrusor; dose: E2 (0.1 mg/kg/day); P4 (1mg/kg/day); P4 and DHEA (300 ug/kg/day);.

16020 Hammond, R., Gibbs, R. B. GPR30 is positioned to mediate estrogen effects on basal forebrain cholinergic neurons and cognitive performance. Brain Res 2011; 1379(--):53-60. >>> estradiol, 17B; G-1; G Protein-couple receptor 30 antagonist; rat; animal info (Ovx Rat); administration of estradiol to perimenopausal women, or women who have gone an ovariectomy, right after, will help with cognitive functions. There is a"window of opportunity to help with cognition. administration of estradiol to womem older than 69 might not be as useful, and even harmful; dose (5ug/day).

15904 Laszlo Prokai,, Vien Nguyen,, Szabolcs Szarka, Puja Garg,, Gauri Sabnis, Heather A. Bimonte-Nelson,, Katie J. McLaughlin,, Joshua S. Talboom,, Cheryl D. Conrad,, Paul J. Shughrue,, Todd D. Gould,, Angela Brodie,, Istvan Merchenthaler,, Peter Koulen,, Katalin Prokai-Tatrai  Science Translational Medicine 2015; 7(-297-):1-11. >>> DHED; estradiol, 17b-; PEG; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Fischer 344, middle aged, OVX); pumps replaced every 14 days; behavioral testing (plus maze); Dose (4 ug/day);.

15884 Scott, E. L., Zhang, Q. G., Han, D., Desai, B. N., Brann, D. W. Long-term estrogen deprivation leads to elevation of Dickkopf-1 and dysregulation of Wnt/beta-Catenin signaling in hippocampal CA1 neurons. Steroids 2013; 78(-6-):624-32. >>> 17 B-Estradiol (E2); Cyclodextrin, B-; SC; rat; 24- month old, female mice, ovariectomized; neurodegenerative (dementia); 20% cyclodextrin used; mp were used to infuse estradiol to mimic physiological levels during diestrus I (10-15 pg/mL); dose: 0.0167 mg.

15849 Yang, J., He, F., Meng, Q., Sun, Y., Wang, W., Wang, C. Inhibiting HIF-1alpha Decreases Expression of TNF-alpha and Caspase-3 in Specific Brain Regions Exposed Kainic Acid-Induced Status Epilepticus. Cell Physiol Biochem 2016; 38(-1-):75-82. >>> Estradiol, 2-methoxy; entanercept; CSF, artificial; CSF/CNS; Rat; 1003D; Controls received mp w/ vehicle; animal info (male, Wistar, 200-250g); ALZET brain infusion kit used; Cannula placement verified via histologic analysis;.

15802 Osamu Nakagawasaia,, Wataru Nemoto,, Hiroshi Onogi,, Takahiro Moriya, Jia-Rong Lin, Takayo Odaira, Fukie Yaoita, Takumi Ogawa, Kiminori Ohta, Yasuyuki Endo, Koichi Tan-No  Behavioural Brain Research 2016; 297(--):315-322. >>> BE360; estradiol, 17b-; tamoxifen; raloxifen hydrochloride; PEG 300; SC; Mice; 2002; Controls received mp w/ vehicle; animal info (female, ddY, 10 weeks old, bulbectomized); dose-response (pg 317-318); neurodegenerative (dementia); behavioral testing (sucrose preference; y-maze); BE360 is a novel selective estrogen receptor modulator; Dose (100 ug/day);.

15797 E. Maggioli, S. McArthur, C. Mauro, J. Kieswich, D.H.M. Kusters, C.P.M. Reutelingsperger, M. Yaqoob, E. Solito  Brain Behav. Immun 2016; 51(--):212-222. >>> Estradiol; Cyclodextrin, B-; SC; Mice; 2002; Controls received mp w/ vehicle; animal info (C57BL6 or AnxA1 -/-, 2 months old, OVX ); replacement therapy (estradiol infusion); immunology; dose: 100nM.

15779 Akihiko Kimura, Yuko Ishida, Mizuho Nosaka, Yumi Kuninaka, Mizuki Hama, Takashi Kawaguchi, Shoichi Sakamoto, Kohei Shinozaki, Yumi Iwahashi, Tatsunori Takayasu, Toshikazu Kondo  Toxicology 2016; 339(--):9-18. >>> Estrogen-17B; Ethanol; PBS; Mice; 1002; Controls received no mp; Controls received no mp; Controls received no mp; replacement therapy (estrogen infusion); Dose (0.2 mg/kg/day);.

15725 Jeffrey A. Blair,, Russell Palm,, Jaewon Chang,, Henry McGee,, Xiongwei Zhu,, Xinglong Wang,, Gemma Casadesus  Horm. Behav 2016; 78(--):60-66. >>> Estradiol, 17b; leuprolide acetate; Saline; SC; Mice; 1004; Controls received mp w/ vehicle; animal info (C57BL6J, 9 months old, OVX); pumps replaced every month; behavioral testing (morris water maze); replacement therapy (estradiol infusion); Dose (E2 1.1 ng/day; leuprolide 3.6 ug/day);.

15249 pelletYu, Z., Tang, L., Chen, L.F., Li, J.F., Wu, W.F., Hu, C.L., Tang, L. Role for HIF-1alpha and Downstream Pathways in Regulating Neuronal Injury after Intracerebral Hemorrhage in Diabetes. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2015; 37(--):67-76. >>> Estradiol, 2-methoxy; ML228; CSF, artificial; Rat; 1003D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 200-300g, STZ); ALZET brain infusion kit used; diabetes;“This intervention allowed animals to receive continuous intracerebroventricular (ICV) infusion via the minipumps before brain tissues were removed. ” pg 69;.

15044 Montoya, T.I., Maldonado, P.A., Acevedo, J.F., Word, R.A., Word, R.A. Effect of Vaginal or Systemic Estrogen on Dynamics of Collagen Assembly in the Rat Vaginal Wall. BIOLOGY OF REPRODUCTION 2015; 92(--):U115-U123. >>> Estradiol; SC; Rat; Animal info (female, Sprague Dawley, 12 weeks old); replacement therapy (estradiol infusion);.

15255 Mennenga, S.E., Gerson, J.E., Koebele, S.V., Kingston, M.L., Tsang, C.W.S., Engler-Chiurazzi, E.B., Baxter, L.C., Bimonte-Nelson, H.A., Bimonte-Nelson, H.A. Understanding the cognitive impact of the contraceptive estrogen Ethinyl Estradiol: Tonic and cyclic administration impairs memory, and performance correlates with basal forebrain cholinergic system integrity. PSYCHONEUROENDOCRINOLOGY 2015; 54(--):1-13. >>> Estradiol, ethinyl; Propylene glycol; SC; Rat; 2006; Controls received mp w/ vehicle; animal info (female, Fischer-344, OVX, 3 months old); functionality of mp verified by serum levels; dose-response (pg8); behavioral testing (water radial-arm maze, morris water maze, visible platform task); replacement therapy (estradiol infusion); long-term study;.

14974 Ikeda, T., Makino, Y., Yamada, M.K., Yamada, M.K. 17alpha-Estradiol is generated locally in the male rat brain and can regulate GAD65 expression and anxiety. NEUROPHARMACOLOGY 2015; 90(--):9-14. >>> Letrizole; estradiol, 17b-; DMSO; saline; CSF/CNS; Rat; Controls received mp w/ vehicle; animal info (male, Wistar/ST, 4 weeks old); 20% DMSO used; behavioral testing (open field test);“For the behavior test, to avoid stress from the daily handling, a tube from the subcutaneously-implanted osmotic pump was connected to the injection needle in the guide cannula for three days.” pg 10; used guide cannula;.

14922 Dong, G.J., Fan, H.Y., Yang, Y.H., Zhao, G.F., You, M., Wang, T.T., Hou, Y.Y., Hou, Y.Y. 17beta-Estradiol enhances the activation of IFN-alpha signaling in B cells by down-regulating the expression of let-7e-5p, miR-98-5p and miR-145a-5p that target IKKepsilon. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2015; 1852(--):1585-1598. >>> Estradiol, 17b-; Ethanol; propylene glycol; SC; Mice; 1004; Controls received mp w/ vehicle; animal info (female, C57BL6, 4 weeks old, OVX); 0.5% ethanol used; 99.5% polypropylene glycol used; replacement therapy (estradiol infusion);.

14762 Weigt, C., Hertrampf', T., Flenker, U., Huelsemann, F., Kurnaz, P., Fritzemeier, K.H., Diel, P., Weigt, C. Effects of estradiol, estrogen receptor subtype-selective agonists and genistein on glucose metabolism in leptin resistant female Zucker diabetic fatty (ZDF) rats. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2015; 154(--):12-22. >>> Estradiol; estrogen receptor selective agonist, alpha; estrogen receptor selective agonist, beta; DMSO; water; Rat; Animal info (juvenile, female, ZDF fa/fa, 6 wks old, 180-240 g).

14531 Zhang, J., Lazarenko, O.P., Blackburn, M.L., Badger, T.M., Ronis, M.J.J., Chen, J.R., Chen, J.R. Soy protein isolate down-regulates caveolin-1 expression to suppress osteoblastic cell senescence pathways. FASEB Journal 2014; 28(--):3134-3145. >>> Estradiol, b-; PEG 400; SC; Rat (pregnant); 2004; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, GD4); replacement therapy (ovarectomized);.

14493 Xue, B.J., Zhang, Z.M., Beltz, T.G., Guo, F., Hay, M., Johnson, A.K., Xue, B.J. Estrogen regulation of the brain renin-angiotensin system in protection against angiotensin II-induced sensitization of hypertension. American Journal of Physiology-Heart and Circulatory Physiology 2014; 307(--):H191-H198. >>> Angiotensin II; estradiol, 17b-; angiotensin (1-7); A-779; Saline; DMSO; SC; CSF/CNS; Rat; 2001; 2002; Animal info (Sprague Dawley, 10-12 weeks old); ALZET brain infusion kit used; Multiple pumps per animal (2); replacement therapy (ovarectomized, estradiol infusion); cardiovascular; antihypertensive; peptides; bp measured using radiotelemetry (DSI);.

14439 Terzian, A.L.B., Micale, V., Wotjak, C.T., Wotjak, C.T. Cannabinoid receptor type 1 receptors on GABAergic vs. glutamatergic neurons differentially gate sex-dependent social interest in mice. European Journal of Neuroscience 2014; 40(--):2293-2298. >>> Estradiol, b-; Ethanol; propylene glycol; Mice; 1002; Animal info (male, CB1 KO, 8-12 weeks old, OVX); 0.5% ethanol used; behavioral testing (social interaction test; three-chamber social investigation test); replacement therapy (ovariectomized, estradiol infusion);.

14392 Scott, E.L., Zhang, Q.G., Dong, Y., Han, D., Wang, R.M., Vadlamudi, R.K., Brann, D.W., Brann, D.W. Surgical menopause enhances hippocampal amyloidogenesis following global cerebral ischemia. JOURNAL OF SPORT AND HEALTH SCIENCE 2014; 3(--):206-216. >>> Estradiol, 17B-; Cyclodextrin, B-; SC; Rat; 2002; Controls received mp w/ vehicle; animal info (female, ovariectomized); 20% cyclodextrin used; ischemia (cerebral); replacement therapy (estradiol infusion); cardiovascular;.

14385 Sarvari, M., Deli, L., Kocsis, P., Mark, L., Maasz, G., Hrabouszky, E., Kallo, I., Gajari, D., Vastagh, C., Suemeg, B., Tihanyi, K., Liposits, Z., Sarvari, M. Estradiol and isotype-selective estrogen receptor agonists modulate the mesocortical dopaminergic system in gonadectomized female rats. Brain Research 2014; 1583(--):1-11. >>> Estradiol; Rat; 2004; Animal info (female, Harlan-Wistar 2 months old, ovariectomized); replacement therapy (estradiol infusion); mentions use of"silicone capsules", but only mentions pumps once;.

14600 Kramer, P.R., Bellinger, L.L., Kramer, P.R. INFUSION OF GABRalpha6 siRNA INTO THE TRIGEMINAL GANGLIA INCREASED THE MYOGENIC OROFACIAL NOCICEPTIVE RESPONSE OF OVARIECTOMIZED RATS TREATED WITH 17beta-ESTRADIOL. Neuroscience 2014; 278(--):144-153. >>> Estradiol benzoate, 17 beta-; SC; Rat; Animal info (Sprague Dawley, 250-300 g, female, ovariectomized); 28 day pump used; hormone replacement (ovariectomy).

13921 Sarvari, M., Kallo, I., Hrabovszky, E., Solymosi, N., Liposits, Z. Ovariectomy and Subsequent Treatment with Estrogen Receptor Agonists Tune the Innate Immune System of the Hippocampus in Middle-Aged Female Rats. PLoS One 2014; 9(-2-):U411-U422. >>> Estradiol, 17b-; agonist, estrogen receptor 16-alpha-lactone; Propylene glycol; SC; Rat; 2004; Controls received mp w/ vehicle or sham operation; animal info (female, Harlan-Wistar, OVX, 13 months old); replacement therapy (ovariectomized; estradiol infusion); immunology; 16-alpha-lactone estrogen agonist aka L2;.

13798 Han, S.I., Komatsu, Y., Murayama, A., Steffensen, K.R., Nakagawa, Y., Nakajima, Y., Suzuki, M., Oie, S., Parini, P., Vedin, L.L., Kishimoto, H., Shimano, H., Gustafsson, J.A., Yanagisawa, J., Yanagisawa, J. Estrogen Receptor Ligands Ameliorate Fatty Liver Through a Nonclassical Estrogen Receptor/Liver X Receptor Pathway in Mice. Hepatology 2014; 59(--):1791-1802. >>> Estradiol, 17B-; phloretin; Mice (transgenic); 2004; Animal info (female, C57BL6 or ERa flox/flox, 5 weeks old); replacement therapy (ovariectomized); phloretin is a phytoestrogen;.

13732 Castello-Ruiz, M., Torregrosa, G., Burguete, M.C., Miranda, F.J., Centeno, J.M., Lopez-Morales, M.A., Gasull, T., Alborch, E., Torregrosa, G. The selective estrogen receptor modulator, bazedoxifene, reduces ischemic brain damage in male rat. Neuroscience Letters 2014; 575(--):53-57. >>> Estradiol, 17B-; bazedoxifene; DMSO; IP; Rat; 2ML1; Controls received mp w/ vehicle; animal info (male, Wistar, 300-350g); functionality of mp verified by plasma levels; 100% DMSO used; ischemia (cerebral, MCAO); cardiovascular;.

13528 Zhang, Q.G., Wang, R.M., Scott, E., Han, D., Dong, Y., Tu, J.Y., Yang, F., Sareddy, G.R., Vadlamudi, R.K., Brann, D.W. Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause. Brain 2013; 136(-;-):1432-1445. >>> Estradiol, 17b-; SC; Rat; Controls received mp w/ placebo; animal info (female, 3 months old, Sprague Dawley, ovariectomized); neurodegenerative (Alzheimer's disease); behavioral testing (Morris Water Maze); replacement therapy (17beta estradiol after ovariectomy);.

13527 Weigt, C., Hertrampf, T., Kluxen, F.M., Flenker, U., Huelsemann, F., Fritzemeier, K.H., Diel, P. Molecular effects of ER alpha- and beta-selective agonists on regulation of energy homeostasis in obese female Wistar rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2013; 377(-1-2-):147-158. >>> Estradiol; agonist, estradiol receptor alpha-selective 16-alpha-VE2; agonist, estradiol receptor beta-selective 8-beta-VE2; DMSO; water; SC; Rat; Controls received sham surgery; animal info (Female, Wistar, 120-150g, 6 weeks old); replacement therapy (ovariectomy, estradiol infusion); long-term study; immunology;.

13526 Miousse, I.R., Gomez-Acevedo, H., Sharma, N., Vantrease, J., Hennings, L., Shankar, K., Cleves, M.A., Badger, T.M., Ronis, M.J. Mammary gland morphology and gene expression signature of weanling male and female rats following exposure to exogenous estradiol. EXPERIMENTAL BIOLOGY AND MEDICINE 2013; 238(-9-):1033-1046. >>> Estradiol, 17b-; PEG; SC; Rat; 2002; Controls received mp w/ vehicle; animal info (Sprague Dawley, 21 days old); functionality of mp verified by serum measurement; dose-response (table 1&2); toxicology;.

13525 Cao, L., Gao, H., Gui, S.B., Bai, G.W., Lu, R.C., Wang, F., Zhang, Y.Z. Effects of the estrogen receptor antagonist fulvestrant on F344 rat prolactinoma models. Journal of Neuro-oncology 2014; 116(-3-):523-531. >>> Estradiol, 17b-; Glycerol; DMSO; SC; Rat; 2006; Controls received mp w/ vehicle; animal info (female, Fischer, 4 weeks old, 60-70g); functionality of mp verified by prolactinoma growth with PRL immunoreactive lactotrophs; 50% DMSO; 50% glycerol used; cancer (prolactinomas); long-term study;.

13102 Balgobin, S., Montoya, T.I., Shi, H.L., Acevedo, J.F., Keller, P.W., Riegel, M., Wai, C.Y., Word, R.A. Estrogen Alters Remodeling of the Vaginal Wall after Surgical Injury in Guinea Pigs. Biology of Reproduction 2013; 89(-6-):U91-U100. >>> Estradiol; Polyethylene glycol; SC; Guinea pig; 2006; Control animals received mp w/ vehicle; animal info (virginal, female, adult, Hartley, 12 wks old); replacement therapy (bilateral oophrectomy).

13033 Basha, M.E., Chang, S., Burrows, L.J., Lassmann, J., Wein, A.J., Moreland, R.S., Chacko, S. Effect of Estrogen on Molecular and Functional Characteristics of the Rodent Vaginal Muscularis. Journal of Sexual Medicine 2013; 10(-5-):1219-1230. >>> Estradiol, 17-; Cyclodextrin; SC; Rat; 2002; Control animals received mp w/ saline; animal info (Sprague Dawley, female, 3-4 mo old, 250-300 g); replacement therapy (ovariectomy).

12970 Chen, W.F., Gao, Q.G., Dai, Z.J., Kung, A.W.C., Guo, D.A., Wong, M.S. Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model. MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY 2012; 19(-9-):1052-1061. >>> Estradiol; Rg1; SC; Mice; Control animals received mp w/ vehicle; animal info (C57BL/6J, female, OVX, 5 mo old); long-term study; replacement therapy (ovariectomy).

12908 Bartell, S.M., Han, L., Kim, H.N., Kim, S.H., Katzenellenbogen, J.A., Katzenellenbogen, B.S., Chambliss, K.L., Shaul, P.W., Roberson, P.K., Weinstein, R.S., Jilka, R.L., Almeida, M., Manolagas, S.C. Non-Nuclear-Initiated Actions of the Estrogen Receptor Protect Cortical Bone Mass. MOLECULAR ENDOCRINOLOGY 2013; 27(-4-):649-656. >>> Estradiol; dendrimer, empty estradiol; IP; Mice; 2006; Control animals received mp w/ vehicle; animal info (C57BL/6, female, 15 wks old); replacement therapy (ovariectomy); long-term study;.

12892 Babbs, R.K., Unger, E.L., Corwin, R.L.W. 2-Hydroxyestradiol enhances binge onset in female rats and reduces prefrontal cortical dopamine in male rats. Hormones and Behavior 2013; 63(-1-):88-96. >>> Estradiol, hydroxy 2-; PEG 400; SC; Rat; 2ML4; Control animals received mp w/ vehicle; animal info (Sprague Dawley, female); replacement therapy (ovariectomy); wound clips used; half-life (pg.89)..

12768 Sarvari, M., Hrabovszky, E., Kallo, I., Solymosi, N., Liko, I., Berchtold, N., Cotman, C., Liposits, Z. Menopause leads to elevated expression of macrophage-associated genes in the aging frontal cortex: rat and human studies identify strikingly similar changes. Journal of Neuroinflammation 2012; 9(-;-):U1-U13. >>> Estradiol, 17b-; Rat; 2004; Control animals received sham operation; animal info (female, Wistar, young, middle-aged); replacement therapy (ovariectomy).

12733 Kang, S.L., Liu, Y., Sun, D., Zhou, C.L., Liu, A.Y., Xu, C.Y., Hao, Y.L., Li, D.Y., Yan, C.D., Sun, H. Chronic Activation of the G Protein-Coupled Receptor 30 with Agonist G-1 Attenuates Heart Failure. PLoS One 2012; 7(-10-):U552-U560. >>> Estradiol; G protein-coupled receptor 30 agonist; SC; Rat; 2006; Animal info (Sprague Dawley, female, 180-220 g); G protein-coupled receptor 30 agonist also known as G15.

12675 Johnson, J.A., Calo, S., Nair, L., IglayReger, H.B., Greenwald-Yarnell, M., Skorupski, J., Myers, MG Jr, Bodary, P.F. Testosterone interacts with the feedback mechanisms engaged by Tyr985 of the leptin receptor and diet-induced obesity. Journal of Steroid Biochemistry and Molecular Biology 2012; 132(-3-5-):212-219. >>> Estradiol; testosterone; Ethanol; propylene glycol; PBS; SC; Mice; 1002; 1004; Control animals received mp w/ vehicle; animal info (LEPR-B Tyr985Leu, male, female, OVX, castrated); 10% ethanol used; replacement therapy (ovariectomy); post op. care (analgesic, carprofen).

12661 Campos, F.L., Cristovao, A.C., Rocha, S.M., Fonseca, C.P., Baltazar, G. GDNF Contributes to Oestrogen-Mediated Protection of Midbrain Dopaminergic Neurones. Journal of Neuroendocrinology 2012; 24(-11-):1386-1397. >>> Oestradiol, 17b-; Ethanol; polypropylene glycol; SC; Rat; 1007D; Control animals received mp w/ vehicle; animal info (Wistar, male, adult, 200-400 g); 0.5% ethanol used; functionality of mp verified via serum drug levels.

12653 Ronis, M.J.J., Shankar, K., Gomez-Acevedo, H., Hennings, L., Singhal, R., Blackburn, M.L., Badger, T.M. Mammary Gland Morphology and Gene Expression Differ in Female Rats Treated with 17beta-Estradiol or Fed Soy Protein Isolate. Endocrinology 2012; 153(-12-):6021-6032. >>> Estradiol; Polyethylene glycol; SC; Rat; 2002; Control animals received mp w/ vehicle; animal info (Sprague Dawley, female); replacement therapy (ovariectomy).

12614 Rabaglino, M.B., Richards, E., Denslow, N., Keller-Wood, M., Wood, C.E. Genomics of estradiol-3-sulfate action in the ovine fetal hypothalamus. PHYSIOLOGICAL GENOMICS 2012; 44(-13-):669-677. >>> Estradiol-3-sulfate; Water; CSF/CNS; Sheep (fetus); 2ML2; Animal info (twin fetuses, 120-127 days of gestation); good methods, pg 669; functionality of mp verified via visual inspection at the time of death and tissue collection.

12427 Zhang, J., Lazarenko, O.P., Wu, X.L., Tong, Y.D., Blackburn, M.L., Gomez-Acevedo, H., Shankar, K., Badger, T.M., Ronis, M.J.J., Chen, J.R. Differential Effects of Short Term Feeding of a Soy Protein Isolate Diet and Estrogen Treatment on Bone in the Pre-Pubertal Rat. PLoS One 2012; 7(-4-):U1022-U1030. >>> Estradiol, 17-beta; SC; Rat; 2004; Animal info (Sprague Dawley, female, pre-pubertal).

12265 Zoth, N., Weigt, C., Zengin, S., Selder, O., Selke, N., Kalicinski, M., Piechotta, M., Diel, P. Metabolic effects of estrogen substitution in combination with targeted exercise training on the therapy of obesity in ovariectomized Wistar rats. Journal of Steroid Biochemistry and Molecular Biology 2012; 130(-1-2-):64-72. >>> Estradiol, 17-beta; Rat; Animal info (Wistar, female, ovariectomized, obese, 400 g); replacement therapy (ovariectomy);.

12122 Pelch, K.E., Carleton, S.M., Phillips, C.L., Nagel, S.C. Developmental Exposure to Xenoestrogens at Low Doses Alters Femur Length and Tensile Strength in Adult Mice. Biology of Reproduction 2012; 86(-3-):U87-U95. >>> Estradiol; Polyethylene glycol; DMSO; SC; Mice; 1002; Controls received mp w/ vehicle; animal info (female, adult, C57BL/6J, ovariectomized); 20% DMSO used; 80% polyethylene glycol used; replacement therapy (ovariectomy); teratology.


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What researchers are saying...

“Since purified FSH has a short half-life, the hormone is administered as regular injection in 12-hr intervals or constant infusion via a subcutaneously implanted osmotic minipump.” Popova et al., Molecular reproduction and development 2002;63:177-182.