References on the Administration of Estrogens Using ALZET^® Osmotic Pumps

Q0892 Sakata,A., Mogi,M., Iwanami,J., Tsukuda,K., Min,L.J., Jing,F., Ohshima,K., Ito,M., Horiuchi,M. Female type 2 diabetes mellitus mice exhibit severe ischemic brain damage. Journal of the American Society of Hypertension 2011; 5(-1-):7-11. >>> Estradiol, beta; Propylene glycol; SC; Mice; 2 weeks; Controls received mp w/ vehicle; animal info (C57BL/6, 10 wks old, KKAy, male, female, OVX); replacement therapy (ovariectomized).

Q0847 Barb,C.R., Hausman,G.J., Kraeling,R.R. Luteinizing hormone secretion as influenced by age and estradiol in the prepubertal gilt. ANIMAL REPRODUCTION SCIENCE 2010; 122(-3-4-):324-327. >>> Estradiol benzoate; Propylene glycol; SC; Pig; 2ML2; ; Controls received mp w/ vehicle; animal info (gilt, OVX, 90, 150, 210 day old); functionality of mp verified by serum estradiol levels; replacement therapy (ovariectomized).

P9910 Burek,M., rias-Loza,P.A., Roewer,N., Foerster,C.Y. Claudin-5 as a Novel Estrogen Target in Vascular Endothelium. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2010; 30(-2-):298-U317. >>> Estradiol; Cyclodextrin; SC; Mice; 1004; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (2 wks old, female, C57BL/6).

Q0109 Chambliss,K.L., Wu,Q.A., Oltmann,S., Konaniah,E.S., Umetani,M., Korach,K.S., Thomas,G.D., Mineo,C., Yuhanna,I.S., Kim,S.H., Madak-Erdogan,Z., Maggi,A., Dineen,S.P., Roland,C.L., Hui,D.Y., Brekken,R.A., Katzenellenbogen,J.A., Katzenellenbogen,B.S., Shaul,P.W. Non-nuclear estrogen receptor-alpha signaling promotes cardiovascular protection but not uterine or breast cancer growth in mice. Journal of Clinical Investigation 2010; 120(-7-):2319-2330. >>> Estradiol; estrogen-dendrimer conjugate; IP; Mice (nude); 3, 7 weeks; 72 hours; Controls received mp w/ empty dendrimer; cardiovascular; cancer (breast); animal info (female, ERE-Luc reporter, 10-13 weeks old; Ex3aERKO, 8-9 weeks old; C57BL/6 Apoe-/-, 6 weeks old; SCID, 8 weeks old); functionality of mp verified by serum agent levels; replacement therapy (ovariectomy; pumps replaced after 28 days); long-term study; photon recording with light emission tomography (LET) system with a CCD camera; Research Diets D10001.

P9962 Guzzo,A.C., Berger,R.G., deCatanzaro,D. Excretion and binding of tritium-labelled oestradiol in mice (Mus musculus): implications for the Bruce effect. REPRODUCTION 2010; 139(-1-):255-263. >>> Oestradiol, tritium labeled; Ethanol; water, distilled; Mice; 1007D; ; Controls received mp w/ vehicle; animal info (male, 10-15 months old, female, CF-1, 3-4 months old).

Q0102 Hutchens,M.P., Nakano,T., Kosaka,Y., Dunlap,J., Zhang,W.R., Herson,P.S., Murphy,S.J., Anderson,S., Hurn,P.D. Estrogen Is Renoprotective via a Nonreceptor-dependent Mechanism after Cardiac Arrest In Vivo. Anesthesiology 2010; 112(-2-):395-405. >>> ICI 182,780; estradiol, 17b-; DMSO; saline; SC; Mice; 1007D; ; Replacement therapy (ovariectomy); animal info (male, female, Er alpha beta ko, wt); 50% DMSO used; "Because we were unable to find precedent in the scientific literature for delivering ICI 182,780 via silastic implant, we used osmotic pumps." pg 397.

Q0126 Johnson,M.L., Ho,C.C., Day,A.E., Walker,Q.D., Francis,R., Kuhn,C.M. Oestrogen Receptors Enhance Dopamine Neurone Survival in Rat Midbrain. Journal of Neuroendocrinology 2010; 22(-4-):226-237. >>> Estradiol, 17b-; diarylpropionitrile; Saline, normal; DMSO; SC; Rat; 2004; 30 days; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 60 days old); replacement therapy (ovariectomy); 50% DMSO; neuroendocrinology.

Q0483 Osako,M.K., Nakagami,H., Koibuchi,N., Shimizu,H., Nakagami,F., Koriyama,H., Shimamura,M., Miyake,T., Rakugi,H., Morishita,R. Estrogen Inhibits Vascular Calcification via Vascular RANKL System Common Mechanism of Osteoporosis and Vascular Calcification. Circulation Research 2010; 107(-4-):466-U57. >>> Estradiol, 17b-; Propylene glycol; Mice; 1004; 3 months; Controls received mp w/saline; animal info (female, ApoE -/-, ovariectomy); long-term study; replacement therapy (ovariectomy).

Q0838 Park,D., Choi,D., Lee,J., Lim,D.S., Park,C. Male-like sexual behavior of female mouse lacking fucose mutarotase. BMC GENETICS 2010; 11(-;-):U1-U12. >>> Estradiol; Sesame oil; SC; Mice; 2004; 3 weeks; Animal info (FucM-/- (KO)); replacement therapy (ovariectomy).

Q0162 Sakata,A., Mogi,M., Iwanami,J., Tsukuda,K., Min,L.J., Jing,F., Iwai,M., Ito,M., Horiuchi,M. Female exhibited severe cognitive impairment in type 2 diabetes mellitus mice. LIFE SCIENCES 2010; 86(-17-18-):638-645. >>> Estradiol, beta-; Propylene glycol; IP; Mice; 1002; ; Replacement therapy (ovariectomy); animal info (8-12 wks old, C57BL/6, KKAY); diabetes.

P9490 Abu-Taha,M., Rius,C., Hermenegildo,C., Noguera,I., Cerda-Nicolas,J.M., Issekutz,A.C., Jose,P.J., Cortijo,J., Morcillo,E.J., Sanz,M.J. Menopause and Ovariectomy Cause a Low Grade of Systemic Inflammation that May Be Prevented by Chronic Treatment with Low Doses of Estrogen or Losartan. Journal of Immunology 2009; 183(-2-):1393-1402. >>> Estradiol, 17b-; benazepril; DMSO; SC; Rat; 2004; ; Controls received mp w/ vehicle or sham operation; animal info (male, female, Sprague Dawley, OVX); 50% DMSO used; dose (5 ug/kg/d).

P9502 Armaiz-Pena,G.N., Mangala,L.S., Spannuth,W.A., Lin,Y.G., Jennings,N.B., Nick,A.M., Langley,R.R., Schmandt,R., Lutgendorf,S.K., Cole,S.W., Sood,A.K. Estrous Cycle Modulates Ovarian Carcinoma Growth. Clinical Cancer Research 2009; 15(-9-):2971-2978. >>> Estradiol, 17b-; progesterone; megestrol acetate; Cyclodextrin, b-; water; SC; Mice (nude); ; Replacement therapy (ovariectomy); animal info (female, athymic); cancer (ovarian).

Q0540 Chen,J.R., Lazarenko,O.P., Haley,R.L., Blackburn,M.L., Badger,T.M., Ronis,M.J.J. Ethanol Impairs Estrogen Receptor Signaling Resulting in Accelerated Activation of Senescence Pathways, Whereas Estradiol Attenuates the Effects of Ethanol in Osteoblasts. Journal of Bone and Mineral Research 2009; 24(-2-):221-230. >>> Estradiol; Ethanol; SC; Rat; ; Controls received mp w/ vehicle; animal info (Female, Sprague-Dawley, 250-260 g); senescence.

Q0950 Dragoo,J.L., Padrez,K., Workman,R., Lindsey,D.R. The effect of relaxin on the female anterior cruciate ligament: Analysis of mechanical properties in an animal model. KNEE 2009; 16(-1-):69-72. >>> Relaxin, recomb. porcine; estradiol, 17-beta; PBS; SC; Guinea pig; 2ML4; 21 days; Animal info (337 g, Dunkin-Hartley); peptides.

Q0118 Hammond,R., Mauk,R., Ninaci,D., Nelson,D., Gibbs,R.B. Chronic treatment with estrogen receptor agonists restores acquisition of a spatial learning task in young ovariectomized rats. Hormones and Behavior 2009; 56(-3-):309-314. >>> Estradiol; diarylpropiolnitrile; G1; propylpyrazole triol; Cyclodextrin, hydroxypropyl beta-; DMSO; SC; Rat; 2006; ; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 3-4 months old, 300-350 g; replacement therapy (ovariectomy); 13.9% or 33.7% DMSO; GPR30 agonist (G-1); ERa agonist (PPT); ERb agonist (DPN); behavioral testing (DPM spatial learning and memory T-maze).

P9899 Higgins,E.W., Rao,A., Baumann,S.S., James,R.L., Kuehl,T.J., Muir,T.W., Pierce,L.M. Effect of estrogen replacement on the histologic response to polypropylene mesh implanted in the rabbit vagina model. American Journal of Obstetrics and Gynecology 2009; 201(-5-):U192-U200. >>> Estradiol, 17b-; PEG 300; SC; Rabbit; 2ML4; 12 weeks; Controls received mp w/vehicle; replacement therapy (ovariectomy); long-term study; pumps replaced at 4 week intervals; animal info (adult, New Zeland, White, female); "ALZET pumps...are precision drug administration tools that conveniently eliminate the need for daily injections and reduce animal handling," pg 505.e2.

P9493 Hirata,M., Inada,M., Matsumoto,C., Takita,M., Ogawa,T., Endo,Y., Miyaura,C. A novel carborane analog, BE360, with a carbon-containing polyhedral boron-cluster is a new selective estrogen receptor modulator for bone. Biochemical and Biophysical Research Communications 2009; 380(-2-):218-222. >>> BE360; estradiol, 17b-; PEG 300; Mice; 4 weeks; Controls received sham operation; animal info (8 wks old, male, female, ddy, OVX, ORX); BE360 is a novel carborane analog.

P9630 Kramer,P.R., Bellinger,L.L. The Effects of Cycling Levels of 17-beta-Estradiol and Progesterone on the Magnitude of Temporomandibular Joint-Induced Nociception. Endocrinology 2009; 150(-8-):3680-3689. >>> Estradiol, 17-beta; PEG; Rat; ; Controls received mp w/vehicle; replacement therapy (ovariectomy); animal info (female, 250g, Sprague Dawley).

Q0536 Lin,J., Steenbergen,C., Murphy,E., Sun,J.H. Estrogen Receptor-beta Activation Results in S-Nitrosylation of Proteins Involved in Cardioprotection. Circulation 2009; 120(-3-):245-U157. >>> Estradiol; DPN; DMSO; saline; SC; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; animal info (C57BL/6J wt, ER-beta knockout, female, 8 wks old); replacement therapy (ovariectomy); 50% DMSO used; DPN (also known as 2,2-bis(4-hydroxyphenyl)-proprionitrile) is an ER-beta selective agonist.

P9730 Mark,P.J., Augustus,S., Lewis,J.L., Hewitt,D.P., Waddell,B.J. Changes in the Placental Glucocorticoid Barrier During Rat Pregnancy: Impact on Placental Corticosterone Levels and Regulation by Progesterone. Biology of Reproduction 2009; 80(-6-):1209-1215. >>> Estradiol; Propylene glycol; SC; Rat (pregnant); ; Replacement therapy (ovariectomy); animal info (Nulliparous, albino, Wistar, 8-12 wks old).

P9963 Puri,J., Hutchins,B., Bellinger,L.L., Kramer,P.R. Estrogen and inflammation modulate estrogen receptor alpha expression in specific tissues of the temporomandibular joint. Reproductive Biology and Endocrinology 2009; 7(-;-):U1-U8. >>> Estradiol benzoate, 17b-; Polyethylene glycol; Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (Sprague Dawley, 230 g.); 14-day pump used.

P9360 Quintela,T., Goncalves,I., Baltazar,G., Alves,C.H., Saraiva,M.J., Santos,C.R.A. 17 beta-Estradiol Induces Transthyretin Expression in Murine Choroid Plexus via an Oestrogen Receptor Dependent Pathway. Cellular and Molecular Neurobiology 2009; 29(-4-):475-483. >>> Estradiol, 17b-; Ethanol; polypropylene glycol; SC; Mice; 1007D; 1 week; Controls received mp w/ vehicle; animal info (OVX, 5 months old); 0.5% ethanol used.

P9855 Singhal,R., Badger,T.M., Ronis,M.J. Estrogenic status modulates the effect of soy on hepatic responses to 7,12-dimethylbenz(a)anthracene (DMBA). TOXICOLOGY AND APPLIED PHARMACOLOGY 2009; 234(-1-):89-97. >>> Estradiol; Polyethylene glycol; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (adult, female, Sprague Dawley, PND30).

Q0640 Singhal,R., Shankar,K., Badger,T.M., Ronis,M.J. Hepatic gene expression following consumption of soy protein isolate in female Sprague-Dawley rats differs from that produced by 17 beta-estradiol treatment. Journal of Endocrinology 2009; 202(-1-):141-152. >>> Estradiol; Polyethylene glycol; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; animal info (adult, female, Sprague-Dawley); replacement therapy (OVX, ovariectomy).

Q0952 Tofovic,S.P., Zhang,X.C., Jackson,E.K., Zhu,H., Petrusevska,G. 2-methoxyestradiol attenuates bleomycin-induced pulmonary hypertension and fibrosis in estrogen-deficient rats. VASCULAR PHARMACOLOGY 2009; 51(-2-3-):190-197. >>> Estradiol, 2-methoxy; Rat; 2ML4; ; Animal info (female, Sprague-Dawley, 200-250 g, bilateral ovariectomy); half-life (p. 193) "between 30 min and 2 h"; replacement therapy (ovariectomy).

P9467 Xue,B.J., Badaue-Passos,D.Jr, Guo,F., Gomez-Sanchez,C.E., Hay,M., Johnson,A.K. Sex differences and central protective effect of 17 beta-estradiol in the development of aldosterone/NaCl-induced hypertension. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1577-H1585. >>> Aldosterone; estradiol, 17b-; ICI 182,780; SC; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy, castration); cardiovascular; multiple pumps per animal (2); animal info (male, Sprague Dawley, 10-12 wks old); cannula placement verified histologically; estrogen receptor antagonist; endocrinology.

Q0823 Zhang,Q.G., Raz,L., Wang,R.M., Han,D., De Sevilla,L., Yang,F., Vadlamudi,R.K., Brann,D.W. Estrogen Attenuates Ischemic Oxidative Damage via an Estrogen Receptor alpha-Mediated Inhibition of NADPH Oxidase Activation. Journal of Neuroscience 2009; 29(-44-):13823-13836. >>> Estradiol, 17-beta; SC; Rat; 7 days; Controls received placebo; animal info (adult, female, Sprague Dawley, 3 mo old); replacement therapy (ovariectomy); "(0.025 mg) of E2 used produces serum E2 levels of ~10 pg/ml, which represents physiological low diestrus I levels of E2" pg 13824.

P9812 Bonacasa,B., Sanchez,M.L., Rodriguez,F., Lopez,B., Quesada,T., Fenoy,F.J., Hernandez,I. 2-Methoxyestradiol attenuates hypertension and coronary vascular remodeling in spontaneously hypertensive rats. Maturitas 2008; 61(-4-):310-316. >>> Methoxyestradiol, 2-; SC; Rat; 2ML4; 8 weeks; Controls were left intact and untreated; pumps replaced after 4 weeks; animal info (male, female, SHR, 10 wks old, OVX); replacement therapy (ovariectomy).

P9287 Brahn,E., Banquerigo,M.L., Lee,J.K., Park,E.J., Fogler,W.E., Plum,S.M. An Angiogenesis Inhibitor, 2-Methoxyestradiol, Involutes Rat Collagen-Induced Arthritis and Suppresses Gene Expression of Synovial Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor. Journal of Rheumatology 2008; 35(-11-):2119-2128. >>> Estradiol, 2-methoxy-; SC; Rat; 2ML4; 18 days; Controls received mp w/ vehicle; comparison of oral gavage vs. mp; animal info (syngeneic LOU, 8-10 wks old, 100-150 g., Collagen-induced arthritis; "Delivery of 60 mg/kg by an osmotic pump, however, was similar in efficacy to 300 mg/kg oral gavage. This result suggests that a steady-state level of 2ME2 achieved equivalent arthritic inhibition at a lower total dose." (p. 2124).

P9314 Goncalves,I., Alves,C.H., Quintela,T., Baltazar,G., Socorro,S., Saraiva,M.J., Abreu,R., Santos,C.R.A. Transthyretin is up-regulated by sex hormones in mice liver. MOLECULAR AND CELLULAR BIOCHEMISTRY 2008; 317(-1-2-):137-142. >>> Estradiol, 17b-; dihydrotestosterone, 5a-; Polypropylene glycol; ethanol; SC; Mice; 1007D; 1 week; Controls received mp w/ vehicle or sham operation; replacement therapy (ovariectomy, orchidectomy); animal info (male, female, 129S1/Sv, 5 months old): 0.5% EtOH.

P9849 Lefter,R., Doan,V.D., Joseph,V. Contrasting effects of estradiol and progesterone on respiratory pattern and hypoxic ventilatory response in newborn male rats. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2008; 164(-3-):312-318. >>> Estradiol; progesterone; Propylene glycol; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (adult, female, male, multiparous, Sprague Dawley); estradiol and progesterone in same pump; replacement therapy (ovariectomy).

P9351 Maia,C.J.B., Socorro,S., Schmitt,F., Santos,C.R.A. STEAP1 is over-expressed in breast cancer and down-regulated by 17 beta-estradiol in MCF-7 cells and in the rat mammary gland. Endocrine 2008; 34(-1-3-):108-116. >>> Estradiol, 17b-; Ethanol; propylene glycol; Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (adult, male, 3 months old, OVX); 0.5% ethanol used.

P9728 Mersereau,J.E., Levy,N., Staub,R.E., Baggett,S., Zogric,T., Chow,S., Ricke,W.A., Tagliaferri,M., Cohen,I., Bjeldanes,L.F., Leitman,D.C. Liquiritigenin is a plant-derived highly selective estrogen receptor-beta agonist. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2008; 283(-1-2-):49-57. >>> Liquiritigenin; estradiol; SC; Mice (nude); 1 month; Controls received mp w/vehicle; cancer (breast); implies using 2ML4 pump (2.5ul/h); Liquiritigenin is a ER-beta selective compound extracted from the root of G. uralensis.

P9161 Ohtomo,T., Uehara,M., Penalvo,J.L., Adlercreutz,H., Katsumata,S., Suzuki,K., Takeda,K., Masuyama,R., Ishimi,Y. Comparative activities of daidzein metabolites, equol and O-desmethylangolensin, on bone mineral density and lipid metabolism in ovariectomized mice and in osteoclast cell cultures. EUROPEAN JOURNAL OF NUTRITION 2008; 47(-5-):273-279. >>> Estradiol, 17B-; SC; Mice; ; Animal info (female, ddY, 8 wks old, OVX).

P8838 Singhal,R., Shankar,K., Badger,T.M., Ronis,M.J. Estrogenic status modulates aryl hydrocarbon receptor - mediated hepatic gene expression and carcinogenicity. Carcinogenesis 2008; 29(-2-):227-236. >>> Estradiol; SC; Rat; 2002; 15 days; Controls received mp w/ PEG; functionality of mp verified by serum E2 levels; replacement therapy (ovariectomy); animal info (female, Sprague Dawley, 50 days old).

P9122 Tofovic,S.P., Zhang,X.C., Zhu,H., Jackson,E.K., Rafikova,O., Petrusevska,G. 2-ethoxyestradiol is antimitogenic and attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling. VASCULAR PHARMACOLOGY 2008; 48(-4-6-):174-183. >>> Isoproterenol; estradiol, 2-ethoxy; estradiol, 2-hydroxy; estradiol, 2-methoxy; HCl; PEG 400; Rat; 2001; 2ML1; 6 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley); estradiol metabolite; cardiovascular.

P9074 Xue,B.J., Zhao,Y.Z., Johnson,A.K., Hay,M. Central estrogen inhibition of angiotensin II-induced hypertension in male mice and the role of reactive oxygen species. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1025-H1032. >>> Tempol; estradiol, 17B-; ICI-182780; SC; CSF/CNS; Mice; 1002; 7, 14 days; ALZET brain infusion kit used; peptides; animal info (male, C57BL/6, 12-16 wks old); location of cannula was verified histologically post mortem.

P8475 Huber,C., Collishaw,S., Mosley,J.R., Reeve,J., Noble,B.S. Selective estrogen receptor modulator inhibits osteocyte apoptosis during abrupt estrogen withdrawal: Implications for bone quality maintenance. Calcified Tissue International 2007; 81(-2-):139-144. >>> Estradiol, 17B-; LY117018; Cyclodextrin, beta-hydroxy; SC; Rat; 7, 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, Sprague-Dawley, 250-270 g).

P8640 Meitzen,J., Moore,I.T., Lent,K., Brenowitz,E.A., Perkel,D.J. Steroid hormones act transsynaptically within the Forebrain to regulate neuronal phenotype and song stereotypy. Journal of Neuroscience 2007; 27(-44-):12045-12057. >>> Dihydrotestosterone; ICI-182,780; estradiol, 17B-; flutamide; DMSO; PEG 300; propanediol, 1, 2-; SC; CSF/CNS (HVC); CSF/CNS (robust nucleus arcopallium); Bird (sparrow); 1007D; 1002; 14, 21 days; Controls received mp w/ vehicle; pumps replaced after 14 days; cyanoacrylate adhesive; animal info (male, Gambel's white crowned, adult); HVC is the proper name for the telencephalic song nucleus; mp placed in custom built backpack strapped to bird's back w/harness made from surgical dressing, and a microcentrifuge tube; "pilot experiments showed that this arrangement kept the pump at its proper operating temperature (37oC), and that the pump retained saline throughout the entire release period." (p. 12047); ICI-182,780 is also known as faslodex.

P8500 Xue,B.J., Pamidimukkala,J., Lubahn,D.B., Hay,M. Estrogen receptor-alpha mediates estrogen protection from angiotensin II- induced hypertension in conscious female. American Journal of Physiology-Heart and Circulatory Physiology 2007; 292(-4-):H1770-H1776. >>> Angiotensin II; Estradiol, 17B-; ICI-182,780; CSF/CNS; SC; Mice; 1002; 14 days; Blood pressure measured; cardiovascular; ALZET brain infusion kit used; peptides; animal info (OVX, WT, and ER knockout); CNS group delivered E2 + ICI.

P8405 Zhang,X.C., Jia,Y.H., Jackson,E.K., Tofovic,S.P. 2-methoxyestradiol and 2-ethoxyestradiol retard the progression of renal disease in aged, obese, diabetic ZSF1 rats. Journal of Cardiovascular Pharmacology 2007; 49(-1-):56-63. >>> Estradiol, 2-hydroxy-; estradiol, 1-methoxy-; PEG 400; Rat; 2ML4; 9 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 32 days; no stress (see pg. 58-9); cardiovascular; animal info (male, obese ZSF1, 35 wks old, 650-375g.); nephrology.

P8164 Barchiesi,F., Jackson,E.K., Fingerle,J., Gillespie,D.G., Odermatt,B., Dubey,R.K. 2-methoxyestradiol, an estradiol metabolite, inhibits neointima formation and smooth muscle cell growth via double blockade of the cell cycle. Circulation Research 2006; 99(-3-):266-274. >>> Estradiol, 2-methoxy-; IV; Rat; 7, 8, 14, days; Controls received mp w/ vehicle; no stress (see pg. 269); cardiovascular; toxicology; animal info (male, Wistar Kyoto, 350-400 grams); balloon carotid artery injury, "treatment with 2-ME was not associated with any toxic adverse effects." (p. 269); estradiol metabolite.

P7573 Mark,P.J., Smith,J.T., Waddell,B.J. Placental and fetal growth retardation following partial progesterone withdrawal in rat pregnancy. PLACENTA 2006; 27(-2-3-):208-214. >>> Estradiol; Propylene glycol; SC; Rat (pregnant); 6 days; Controls received sham operation and 2x daily injection of vehicle; functionality of mp verified by plasma estradiol concentrations; replacement therapy (ovariectomy); animal info (female, albino Wistar, 3-5 month old).

P7993 Vandenberg,L.N., Wadia,P.R., Schaeberle,C.M., Rubin,B.S., Sonnenschein,C., Soto,A.M. The mammary gland response to estradiol: Monotonic at the cellular level, non-monotonic at the tissue-level of organization?. Journal of Steroid Biochemistry and Molecular Biology 2006; 101(-4-5-):263-274. >>> Estradiol, 17B-; DMSO; SC; Mice; 1002; 10 days; Controls received mp w/ vehicle; replacement therapy (OVX); dose-response (267); animal info (female, CD-1, OVX); 50% DMSO used.

P7634 Andersson,N., Islander,U., Egecioglu,E., Loef,E., Swanson,C., Moverare-Skrtic,S., Sjoegren,K., Lindberg,M.K., Carlsten,H., Ohlsson,C. Investigation of central versus peripheral effects of estradiol in ovariectomized mice. Journal of Endocrinology 2005; 187(-2-):303-309. >>> Estradiol, 17B-; Cyclodextrin, hydroxypropyl, beta; CSF/CNS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; ALZET Brain Infusion Kit used; multiple pumps per animal (2) used; animal info (F, OVX, C57BL/6, 8 weeks old); SC and ICV administration performed concomitantly; 20% cyclodextrin used.

P7601 Batista,M.R., Smith,M.S., Snead,W.L., Connolly,C.C., Lacy,D.B., Moore,M.C. Chronic estradiol and progesterone treatment in conscious dogs: effects on insulin sensitivity and response to hypoglycemia. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2005; 289(-4-):R1064-R1073. >>> Estradiol; Saline; SC; Dog; 2ML4; 3 weeks; Controls received mp w/ vehicle; no stress (see pg. R1065); animal info (female, mongrel, 1 yr, hypoglycemia).

P7216 Diel,P., Laudenbach-Leschowsky,U., Friedel,A., Voss,A., Roussel,J. Pulsed estradiol exposure has a limited ability to induce uterine proliferation in ovariectomised female Wistar rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2005; 230(-1-2-):7-15. >>> Estradiol, 17B-; Saline; ethanol; SC; Rat; 10, 20 days; Replacement therapy (ovariectomy); dose-response (fig. 2); comparison of pellets vs. sc injections vs. IV injections vs. mp; "SC administration of E2 by micropump was chosen... as it provided the most constant E2 serum levels" (p. 11); animal info (F, Wistar, 130 g, OVX).

P7678 Guan,G.Q., Kerins,C.C., Bellinger,L.L., Kramer,P.R. Estrogenic effect on swelling and monocytic receptor expression in an arthritic temporomandibular joint model. Journal of Steroid Biochemistry and Molecular Biology 2005; 97(-3-):241-250. >>> Estradiol, 17B-; PEG; Rat; 2002; 14 days; Animal info (OVX, Sprague-Dawley, 200-225 g, female).

P7549 Munoz-de-Toro,M., Markey,C.M., Wadia,P.R., Luque,E.H., Rubin,B.S., Sonnenschein,C., Soto,A.M. Perinatal exposure to bisphenol-A alters peripubertal mammary gland development in mice. Endocrinology 2005; 146(-9-):4138-4147. >>> Bisphenol A; estradiol; DMSO; SC; Mice (pregnant); 10, 14 days; Controls received mp w/ vehicle; dose-response (fig. 1); cancer (mammary); teratology; animal info (female, CD-1, 9 wk old).

P7188 Nawate,S., Fukao,M., Sakuma,I., Soma,T., Nagai,K., Takikawa,O., Miwa,S., Kitabatake,A. Reciprocal changes in endothelium-derived hyperpolarizing factor-and nitric oxide-system in the mesenteric artery of adult female rats following ovariectomy. British Journal of Pharmacology 2005; 144(-2-):178-189. >>> Estradiol, 17B-; SC; Rat; 2ML4; 4 weeks; Controls received sham surgery; functionality of mp verified by agent plasma levels; replacement therapy (ovariectomy); cardiovascular; animal info (adult, female, OVX, Wistar, 40 weeks old).

P7200 Robichaud,M., Debonnel,G. Oestrogen and testosterone modulate the firing activity of dorsal raphe nucleus serotonergic neurones in both male and female rats. Journal of Neuroendocrinology 2005; 17(-3-):179-185. >>> Estradiol, 17B-; testosterone; progesterone; pregnane-3, 20 dione, 5B-; pregnane-3a-ol, 20-one, 5a-; dehydroepiandrosterone; testosterone, 5a-dihydroxy; Ethanol; water, distilled; CSF/CNS; Rat; 2ML1; 3, 7 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; 3% ethanol; animal info (Sprague-Dawley, 250-325 g).

P7644 Wang,X.X., Wu,J., Chiba,H., Yamada,K., Ishimi,Y. Puerariae radix prevents bone loss in castrated male mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2005; 54(-11-):1536-1541. >>> Estradiol, 17B-; SC; Mice; 4 weeks; Controls received sham surgery with no mp; replacement therapy (orchidectomy); animal info (male, ddY, 8 week old).

P6356 Cheng,G.J., Zhang,W.H., Warner,M., Gustafsson,J.A. Estrogen receptors ER alpha and ER beta in proliferation in the rodent mammary gland. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-11-):3739-3746. >>> Estradiol, 17B-; tamoxifen; Intralipid; IP; Mice; 2004; 48 hours; Controls received mp w/ vehicle; replacement therapy (ovariectomy).

P6260 Gibbs,R.B., Gabor,R., Cox,T., Johnson,D.A. Effects of raloxifene and estradiol on hippocampal acetylcholine release and spatial learning in the rat. Psychoneuroendocrinology 2004; 29(-6-):741-748. >>> Raloxifene; Estradiol, 17B-; DMSO; cyclodextrin, hydroxypropyl-beta (molecusol); SC; Rat; 2004; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); pumps replaced every 28 days; 20% cyclodextrin&1% DMSO used; incorrectly states pumping rate as 2.5 ul/hr; behavioral study; research diets.

P7018 Hillisch,A., Peters,O., Kosemund,D., Mueller,G., Walter,A., Schneider,B., Reddersen,G., Elger,W., Fritzmeier,K.H. Dissecting physiological roles of estrogen receptor alpha and beta with potent selective ligands from structure-based design. MOLECULAR ENDOCRINOLOGY 2004; 18(-7-):1599-1609. >>> Estradiol, 17B-; estrogen receptor alpha ligand; estrogen receptor beta ligand; Propylene glycol; SC; Rat; 2001; 2004; 8,29 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); dose-response (fig 6); ERalpha ligand is an E2-1b alpha-lactone derivative (16a-LE2), ER beta ligand an E2-8 beta-vinylestra derivative (8B-VE2); E2 is 17B-estradiol; pump incorrectly labeled as a 2001D; animal info (adult, OVX, female, Wistar, 220-250 g).

P6517 Kim,N.N., Min,K., Pessina,M.A., Munarriz,R., Goldstein,I., Traish,A.M. Effects of ovariectomy and steroid hormones on vaginal smooth muscle contractility. International Journal of Impotence Research 2004; 16(-1-):43-50. >>> Testosterone; estradiol; dihydrotestosterone; dehydroepiandrosterone; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX (for some)).

P7076 Kim,S.W., Kim,N.N., Jeong,S.J., Munarriz,R., Goldstein,I., Traish,A.M. Modulation of rat vaginal blood flow and estrogen receptor by estradiol. Journal of Urology 2004; 172(-4-):1538-1543. >>> Estradiol, 17B-; PEG 300; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, 12-16 weeks old, 250-300g, Sprague-Dawley).

P6273 Traish,A.M., Huang,Y.H., Min,K.S., Kim,N.N., Munarriz,R., Goldstein,I. Binding characteristics of [3H]Delta5-androstene-3beta 17beta-diol to a nuclear protein in the rabbit vagina. Steroids 2004; 69(-1-):71-78. >>> Estradiol; testosterone; PEG; SC; Rabbit; 2002; 2 weeks; Replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX).

P7084 Wu,J., Wang,X.X., Chiba,H., Higuchi,M., Nakatani,T., Ezaki,O., Cui,H.B., Yamada,K., Ishimi,Y. Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in ovariectomized mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2004; 53(-7-):942-948. >>> Estradiol, 17B-; SC; Mice; 6 weeks; Controls received no treatment; replacement therapy (ovariectomy); animal info (adult, female, ddYstrain, 27 g)).

P6259 Yokota,T., Honda,K., Tsuruya,Y., Nomiya,M., Yamaguchi,O., Gotanda,K., Constantinou,C.E. Functional and anatomical effects of hormonally induced experimental prostate growth: A urodynamic model of benign prostatic hyperplasia (BPH) in the beagle. PROSTATE 2004; 58(-2-):156-163. >>> Dihydrotestosterone, 5 alpha-; Estradiol, 17B-; SC; Dog; 2ML4; 28 days; Animal info (Beagle, 3.5-7.2 yrs); testosterone induced BPH animal model.

P5951 Comeau,L.A., Campana,S.E. Modifying thyroidal status in Atlantic cod by osmotic pump delivery of thyroid and antithyroid agents. TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY 2003; 132(-5-):1021-1026. >>> Triiodothyronine; iodide, potassium; methimazole; Estradiol, 17B-; testosterone; thiourea; Saline; IP; Fish (cod); 2ML1; 17 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; drug plasma levels taken; potassium iodide, methimazole and thiourea are thyroid inhibitors; sex hormones were in a separate study where the ALZET pump model was not listed; "this study demonstrates the value of osmotic pumps as effective delivery vehicles for drugs in wild demersal fish." p. 1024.

P5894 Jalouli,M., Carlsson,L., Ameen,C., Linden,D., Ljungberg,A., Michalik,L., Eden,S., Wahli,W., Oscarsson,J. Sex difference in hepatic peroxisome proliferator-activated receptor alpha expression: Influence of pituitary and gonadal hormones. Endocrinology 2003; 144(-1-):101-109. >>> Growth hormone, bovine; testosterone; estradiol, 17B-; Phosphate buffer; glycerol; sodium asize; propylene glycol; SC; Rat; 2001; 7 days; Replacement therapy (gonadectomy); comparison of daily injections vs. chronic mp; peptides; GH was recomb bovine&diluted in 0.05 m phosphate buffer, ph 8.6, with 1.6% glycerol&0.02% sodium azide; testosterone&estradiol were diluted in propylene glycol.

P5700 Jesmin,S., Hattori,Y., Sakuma,I., Liu,M.Y., Mowa,C.N., Kitabatake,A. Estrogen deprivation and replacement modulate cerebral capillary density with vascular expression of angiogenic molecules in middle-aged female rats. Journal of Cerebral Blood Flow and Metabolism 2003; 23(-2-):181-189. >>> Estradiol, 17B-; SC; Rat; 2ML4; ; Replacement therapy (ovariectomy).

P6588 Min,K., Munarriz,R., Kim,N.N., Choi,S., O'Connell,L., Goldstein,I., Traish,A.M. Effects of ovariectomy and estrogen replacement on basal and pelvic nerve stimulated vaginal lubrication in an animal model. JOURNAL OF SEX&MARITAL THERAPY 2003; 29(-1-):77-84. >>> Estradiol; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); post op. care (butorphanol tartrate, cephazolin).

P5931 Momoi,H., Ikomi,F., Ohhashi,T. Estrogen-induced augmentation of endothelium- dependent nitric oxide-mediated vasodilation in isolated rat cerebral small arteries. Japanese Journal of Physiology 2003; 53(-3-):193-203. >>> Estradiol, 17B-; Polypropylene glycol; SC; Rat; 2002; 7 days; Replacement therapy (ovariectomy).

P5927 Shao,T.C., Li,H.L., Eid,W., Ittmann,M., Unni,E., Cunningham,G.R. In vivo preservation of steroid specificity in CWR22 xenografts having a mutated androgen receptor. PROSTATE 2003; 57(-1-):1-7. >>> Testosterone; estradiol; progesterone; flutamide; Cylodextrin, 2-beta-hydroxypropl; SC; Mice (nude); 2004; 4 weeks; Replacement therapy (castration); cancer (prostate); CWR22 xenograft used; flutamide is an anti-androgen; animal info (5-6 week old, nude ,ORX).

P5732 Sims,N.A., Clement-Lacroix,P., Minet,D., Fraslon-Vanhulle,C., Gaillard-Kelly,M., Resche-Rigon,M., Baron,R. A functional androgen receptor is not sufficient to allow estradiol to protect bone after gonadectomy in estradiol receptor-deficient mice. Journal of Clinical Investigation 2003; 111(-9-):1319-1327. >>> Genistein; Estradiol, 17B-; DMSO; PEG 300; SC; Mice; 2002; ; Replacement therapy (orchidectomy); dose-response (p.336); 20% DMSO used in vehicle.

P5163 Earley,S., Resta,T.C. Estradiol attenuates hypoxia-induced pulmonary endothelin-1 gene expression. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 2002; 283(--):L86-L93. >>> Estradiol, 17B-; Propanediol, 1,2-; Ethanol; SC; rat; 2002; 2ML4; 4 weeks; 24 hours; controls received mp w/ vehicle; replacement therapy (ovariectomy, p. L87); vehicle was 97% 1,2-propanediol and 3% ethanol; 2002 used for 24 hr infusion.

P5853 Golding,E.M., Ferens,D.M., Marrelli,S.P. Altered calcium dynamics do not account for attenuation of endothelium-derived hyperpolarizing factor-mediated dilations in the female middle cerebral artery. Stroke 2002; 33(-12-):2972-2977. >>> Estradiol, 17B-; DMSO; NaCl; SC; Rat; 2004; ; Functionality of mp verified by 17ß-Estradiol plasma levels (p.2974); replacement therapy (ovariectomy); 50% DMSO used.

P5347 Ishimi,Y., Yoshida,M., Wakimoto,S., Wu,J., Chiba,H., Wang,X., Takeda,K., Miyaura,C. Genistein, a soybean isoflavone, affects bone marrow lymphopoiesis and prevents bone loss in castrated male mice. Bone 2002; 31(-1-):180-185. >>> Genistein; estradiol, 17B-; DMSO; PEG 300; SC; Mice; 2002; ; Controls received mp w/ vehicle.

P5138 Kawamoto,S., Ejiri,S., Nagaoka,E., Ozawa,H. Effects of oestrogen deficiency on osteoclastogenesis in the rat periodontium. Arch Oral Biol 2002/1; 47(--):67-73. >>> Estradiol, 17B-; Propylene glycol; SC; rat; 2002; 7 or 14 days; controls received mp w/ vehicle; replacement therapy (ovariectomy p. 68).

P5377 Liang,Y.Q., Akishita,M., Kim,S., Ako,J., Hashimoto,M., Iijima,K., Ohike,Y., Watanabe,T., Sudoh,N., Toba,K., Yoshizumi,M., Ouchi,Y. Estrogen receptor beta is involved in the anorectic action of estrogen. International Journal of Obesity 2002; 26(-8-):1103-1109. >>> Estradiol, 17B-; Oligonucleotide, antisense; Cyclodextrin, 2-hydroxypropyl-beta (molecusol)-; fluorescein isothiocyanate; CSF/CNS; Rat; 2002; 3 weeks; Replacement therapy (ovariectomy); pumps replaced after one week; ALZET brain infusion kit used; initial pump delivered saline only for one week to allow for surgical recovery prior to 2 weeks of hormone administration.

P6166 Min,K., Munarriz,R., Kim,N.N., Goldstein,I., Traish,A. Effects of ovariectomy and estrogen and androgen treatment on sildenaf il-mediated changes in female genital blood flow and vaginal lubricati on in the animal model. American Journal of Obstetrics and Gynecology 2002; 187(-5-):1370-1376. >>> Estradiol; testosterone; PEG; SC; Rabbit; 2002; 14 days; Controls received mp w/ vehicle; estradiol plasma levels taken; replacement therapy (ovariectomy); multiple pumps per animal used (2) for estradiol and testosterone group; animal info (female, New Zealand, white, 3.5-4.0 kg).

P5431 Mukundan,H., Resta,T.C., Kanagy,N.L. 17beta-Estradiol decreases hypoxic induction of erythropoietin gene expression. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2002; 283(-2-):R496-R504. >>> Estradiol, 17B-; Polypropylene glycol; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy).

P5120 Sahlin,L., Elger,W., Hedden,A., Lindberg,M., Reddersen,G., Schneider,B., Schwarz,S., Freyschuss,B., Eriksson,H. Effects of estradiol and estradiol sulfamate on the liver of ovariectomized or ovariectomized and hypophysectomized rats. Journal of Steroid Biochemistry and Molecular Biology 2002; 80(--):457-467. >>> Estradiol, 17B-; Estradiol sulfamate; Dexamethasone; Growth hormone, human; Propylene glycol; SC; rat; 2001; 7 days; controls received mp w/ vehicle; replacement therapy (ovariectomy, hypophysectomy, p. 458); comparison of oral administration vs. mp; steroid administered in PG, human GH pre-formulated.

P5399 Tofovic,S.P., Dubey,R., Salah,E.M., Jackson,E.K. 2-hydroxyestradiol attenuates renal disease in chronic puromycin aminonucleoside nephropathy. Journal of the American Society of Nephrology 2002; 13(-11-):2737-2747. >>> Estradiol, 2-hydroxy-; PEG 400; Rat; 2ML4; 12 weeks.

P5401 Van Abel,M., Hoenderop,J.G.J., Dardenne,O., St Arnaud,R., Van Os,C.H., Van Leeuwen,H.J.P.T., Bindels,R.J.M. 1,25-dihydroxyvitamin D-3-independent stimulatory effect of estrogen on the expression of ECaC1 in the kidney. Journal of the American Society of Nephrology 2002; 13(-8-):2102-2109. >>> Estradiol, 17B-; DMSO; ethanol; water; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; vehicle composed of 50% DMSO, 15% ethanol; 35% water.

P5164 Wang,X., Abdel-Rahman,A.A. Estrogen modulation of eNOS activity and its association with caveolin-3 and calmodulin in rat hearts. American Journal of Physiology-Heart and Circulatory Physiology 2002; 282(--):H2309-H2315. >>> Estradiol, 17B-; Saline; SC; rat; 2004; 12 weeks; controls received mp w/ vehicle; functionality of mp verified by plasma estradiol levels; replacement therapy (ovariectomy); long-term study, pumps replaced every 4 wks; cardiovascular.

P4901 Heaney,A.P., Nelson,V., Fernando,M., Horwitz,G. Transforming events in thyroid tumorigenesis and their association with follicular lesions. Journal of Clinical Endocrinology and Metabolism 2001; 86(-10-):5025-5032. >>> Estradiol, 17B-; Tamoxifen, 4-hydroxy-; PEG; Ethanol; SC; rat; ; Functionality of mp verified by serum estrogen levels; replacement therapy (ovariectomy); vehicle was 90% polyethylene glycol, 10% ethanol; 100 ul pumps used, no model or duration specified..

P4985 Mills,L.H., Lee,A.J., Parlow,A.F., Zhu,B.T. Preferential growth stimulation of mammary glands over uterine endometrium in female rats by a naturally occurring estradiol-17 beta-fatty acid ester. Cancer Research 2001; 61(--):5764-5770. >>> Estradiol, 17B-; Estradiol, 17B- S; Ethanol; DMSO; SC; rat; 1002; 2004; 10 or 23 days; controls received mp w/ vehicle; functionality of mp verified by plasma estradiol levels; replacement therapy (ovariectomy); dose-response (table 1 p. 5768); cancer; vehicle was 70% ethanol, 30% DMSO; 2nd experiment used 2004 pumps for 23 days;.

P4689 Kinuta,K, Tanaka,H, Moriwake,T, Aya,K, Kato,S, Seino,Y  Endocrinology 2000; 141(-4-):1317-1324. >>> Estradiol, 17B-;; mice;; 5 weeks;; Functionality of mp verified by serum estradiol level; comparison of IP injections vs. mp; pumps implanted in 5 - 10 week old mice;.