References Which Document ALZET® Osmotic Pump Functionality

Q0737 Pineda,E.A., Hensler,J.G., Sankar,R., Shin,D., Burke,T.F., Mazarati,A.M. Plasticity of Presynaptic and Postsynaptic Serotonin 1A Receptors in an Animal Model of Epilepsy-Associated Depression. Neuropsychopharmacology 2011; 36(-6-):1305-1316. >>> Mifepristone; DMSO; CSF/CNS (dorsal raphe); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Wistar, 50 days old); functionality of mp verified by residual volume; 10% DMSO used.

Q0847 Barb,C.R., Hausman,G.J., Kraeling,R.R. Luteinizing hormone secretion as influenced by age and estradiol in the prepubertal gilt. ANIMAL REPRODUCTION SCIENCE 2010; 122(-3-4-):324-327. >>> Estradiol benzoate; Propylene glycol; SC; Pig; 2ML2; ; Controls received mp w/ vehicle; animal info (gilt, OVX, 90, 150, 210 day old); functionality of mp verified by serum estradiol levels; replacement therapy (ovariectomized).

Q0002 Batista,D.G., Neves,K.R., Graciolli,F.G., dos Reis,L.M., Graciolli,R.G., Dominguez,W.V., Neves,C.L., Magalhaes,A.O., Custodio,M.R., Moyses,R.M., Jorgetti,V. The Bone Histology Spectrum in Experimental Renal Failure: Adverse Effects of Phosphate and Parathyroid Hormone Disturbances. Calcified Tissue International 2010; 87(-1-):60-67. >>> Parathyroid hormone, recomb. rat (1-34); Cysteine; SC; Rat; 2ML4; 56 days; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, 280-330 g); functionality of mp verified by hormone serum levels; replacement therapy (parathyroidectomy, nephrecotomy (5/6)); pumps replaced after 28 days; long-term study.

Q0109 Chambliss,K.L., Wu,Q.A., Oltmann,S., Konaniah,E.S., Umetani,M., Korach,K.S., Thomas,G.D., Mineo,C., Yuhanna,I.S., Kim,S.H., Madak-Erdogan,Z., Maggi,A., Dineen,S.P., Roland,C.L., Hui,D.Y., Brekken,R.A., Katzenellenbogen,J.A., Katzenellenbogen,B.S., Shaul,P.W. Non-nuclear estrogen receptor-alpha signaling promotes cardiovascular protection but not uterine or breast cancer growth in mice. Journal of Clinical Investigation 2010; 120(-7-):2319-2330. >>> Estradiol; estrogen-dendrimer conjugate; IP; Mice (nude); 3, 7 weeks; 72 hours; Controls received mp w/ empty dendrimer; cardiovascular; cancer (breast); animal info (female, ERE-Luc reporter, 10-13 weeks old; Ex3aERKO, 8-9 weeks old; C57BL/6 Apoe-/-, 6 weeks old; SCID, 8 weeks old); functionality of mp verified by serum agent levels; replacement therapy (ovariectomy; pumps replaced after 28 days); long-term study; photon recording with light emission tomography (LET) system with a CCD camera; Research Diets D10001.

Q0110 Chan,E.C., Dusting,G.J., Guo,N., Peshavariya,H.M., Taylor,C.J., Dilley,R., Narumiya,S., Jiang,F. Prostacyclin receptor suppresses cardiac fibrosis: Role of CREB phosphorylation. Journal of Molecular and Cellular Cardiology 2010; 49(-2-):176-185. >>> Angiotensin II; Saline; SC; Mice; 2 weeks; Controls received mp w/ vehicle; peptides; no stress (pg. 178); animal info (C57BL/6, ApoE-/-, IP-/-, IP+/+, 16-18 weeks old); functionality of mp verified by systolic blood pressure; dose-response (Table 1).

Q0148 Chen,J.J., Lue,J.H., Lin,L.H., Huang,C.T., Chiang,R.P.Y., Chen,C.L., Tsai,Y.J. Effects of pre-emptive drug treatment on astrocyte activation in the cuneate nucleus following rat median nerve injury. Pain 2010; 148(--):158-166. >>> Fluorocitrate; CSF/CNS (cuneate nucleus); Rat; 2004; ; Controls received mp w/ vehicle; functionality of mp verified by residual volume; animal info (male, Sprague Dawley, 180-250 g.); 5 day surgical recovery period.

Q0008 Cho,Y.K., Eom,G.H., Kee,H.J., Kim,H.S., Choi,W.Y., Nam,K.I., Ma,J.S., Kook,H. Sodium Valproate, a Histone Deacetylase Inhibitor, but Not Captopril, Prevents Right Ventricular Hypertrophy in Rats. CIRCULATION JOURNAL 2010; 74(-4-):760-770. >>> Captopril; DMSO; SC; Rat; 14 days; Cardiovascular; enzyme inhibitor (ACE); animal info (male, Sprague-Dawley, 3 weeks old); functionality of mp verified by plasma ang I and ang II levels; right ventricular hypertrophy by pulmonary artery banding.

Q0112 de Oliveira,E., Moura,E.G., Santos-Silva,A.P., Pinheiro,C.R., Lima,N.S., Nogueira-Neto,J.F., Nunes-Freitas,A.L., breu-Villaca,Y., Passos,M.C.F., Lisboa,P.C. Neonatal nicotine exposure causes insulin and leptin resistance and inhibits hypothalamic leptin signaling in adult rat offspring. Journal of Endocrinology 2010; 206(-1-):55-63. >>> Nicotine, free base; Saline; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; teratology; functionality of mp verified by serum and milk cotinine levels; animal info (female, Wistar, lactating); endocrinology.

Q0042 Fujimura,Y., Zoghbi,S.S., Simeon,F., Taku,A., Victor,P., Innis,R., Fujita,M. Quantification of peripheral benzodiazepine receptors in human brain with 18F-PBR06. Journal of Cerebral Blood Flow and Metabolism 2010; 29(--):S360-S375. >>> Nicotine; Monkey (baboon); 6 months; Long-term study; animal info (Papio anubis); functionality of mp verified by plasma nicotine concentration; pumps replaced at 15 days, 2, 3, 4 and 5 months.

Q0122 Huang,X.R., Chung,A.C.K., Yang,F.Y., Yue,W.S., Deng,C.X., Lau,C.P., Tse,H.F., Lan,H.Y. Smad3 Mediates Cardiac Inflammation and Fibrosis in Angiotensin II-Induced Hypertensive Cardiac Remodeling. Hypertension 2010; 55(-5-):1165-U153. >>> Angiotensin II; Saline; SC; Mice; 14 days; Controls received mp w/ vehicle; peptides; cardiovascular; animal info (C57BL/6 wt, Smad3 KO); functionality of mp verified by systolic blood pressure.

Q0085 Knight,Z.A., Hannan,K.S., Greenberg,M.L., Friedman,J.M. Hyperleptinemia Is Required for the Development of Leptin Resistance. PLoS One 2010; 5(-6-):U248-U255. >>> Leptin, recomb.; PBS; SC; Mice; 1004; 2002; 2004; 20 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks, or 12 days; animal info (male, ob/ob C57BL/6J, 4 wks old); functionality of mp verified by plasma leptin levels; "We found, consistent with previous studies, that leptin replacement in ob/ob animals is sufficient to normalize their body weight and other physiologic abnormalities, and furthermore that this normalization can be maintained for 6 months by replacing the pumps monthly." pg. e11377; obesity.

Q0498 Mazarati,A.M., Pineda,E., Shin,D., Tio,D., Taylor,A.N., Sankar,R. Comorbidity between epilepsy and depression: Role of hippocampal interleukin-1beta. NEUROBIOLOGY OF DISEASE 2010; 37(-2-):461-467. >>> Interleukin-1, receptor antagonist, human, recomb.; Saline, sterile; CSF/CNS (hippocampus); Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit 1 used; multiple pumps per animal (2); animal info (male, Wistar, 50 days old); bilateral hippocampal infusion.

P9909 Morris,M.J., Na,E.S., Johnson,A.K. Mineralocorticoid Receptor Antagonism Prevents Hedonic Deficits Induced by a Chronic Sodium Appetite. Behavioral Neuroscience 2010; 124(-2-):211-224. >>> RU 28318; RU 486; CSF/CNS (intracranial); Rat; 2004; ; Animal info (male, Sprague Dawley, 250-375 g); silastic tubing used; "A better strategy may have been to chronically infuse CAP via osmotic pumps." pg 220; functionality of mp verified by residual volume.

Q0179 Oliveira,E., Pinheiro,C.R., Santos-Silva,A.P., Trevenzoli,I.H., breu-Villaca,Y., Neto,J.F.N., Reis,A.M., Passos,M.C.F., Moura,E.G., Lisboa,P.C. Nicotine exposure affects mother's and pup's nutritional, biochemical, and hormonal profiles during lactation in rats. Journal of Endocrinology 2010; 205(-2-):159-170. >>> Nicotine, free base; Saline, physiological; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (female, Wistar, 3 months old, lactating); functionality of mp verified by serum cotinine levels; endocrinology.

Q0026 Owens,AP III, Subramanian,V., Moorleghen,J.J., Guo,Z.H., McNamara,C.A., Cassis,L.A., Daugherty,A. Angiotensin II Induces a Region-Specific Hyperplasia of the Ascending Aorta Through Regulation of Inhibitor of Differentiation 3. Circulation Research 2010; 106(-3-):611-U108. >>> Angiotensin II; norepinephrine; losartan; Saline; Mice; 4 weeks; 28 days; Controls received mp w/vehicle; peptides; cardiovascular; animal info (male, C57BL/6, AT1aR -/-, p47phox -/-, Id3 -/-); functionality of mp verified by systolic blood pressure.

Q0027 Paglialunga,S., Fisette,A., Munkonda,M., Gao,Y., Richard,D., Cianflone,K. The effects of acylation stimulating protein supplementation VS antibody neutralization on energy expenditure in wildtype mice. BMC PHYSIOLOGY 2010; 10(-;-):U1-U11. >>> Antibody, anti- acylation stimulating protein; acylation stimulating protein, recomb. human; immunoglobulin G; PBS; SC; Mice; 2004; 4 weeks; Controls received mp w/IgG; peptides; animal info (male, C57BL/6, 7-8 weeks old); functionality of mp verified by residual volume.

Q0180 Pierce,A.A., Xu,A.W. De Novo Neurogenesis in Adult Hypothalamus as a Compensatory Mechanism to Regulate Energy Balance. Journal of Neuroscience 2010; 30(-2-):723-730. >>> Ara-C; uridine, 5' bromodeoxy; CSF, artificial; BSA; CSF/CNS; Mice (transgenic); 2006; 4, 6 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit; animal info (male, wt, AgRP-Tfam, 12 weeks old); functionality of mp verified by residual volume; cannula placement confirmed histologically.

Q0095 Schmidt,N.O., Ziu,M., Cargioli,T., Westphal,M., Giese,A., Black,P.M., Carroll,R.S. Inhibition of thromboxane synthase activity improves glioblastoma response to alkylation chemotherapy. Translational Oncology 2010; 3(-1-):43-49. >>> Furegrelate; PBS; CSF/CNS (intratumoral); Mice (nude); 2004; 4 weeks; Controls received mp w/ vehicle; tissue perfusion (tumor); functionality of mp verified by residual volume; stress/adverse reaction: (see pg. 46) "hemosiderin deposits"; cancer (glioma); ALZET brain infusion kit used; animal info (Swiss, 4-6 wks old).

Q0186 Schopfer,F.J., Cole,M.P., Groeger,A.L., Chen,C.S., Khoo,N.K.H., Woodcock,S.R., Golin-Bisello,F., Motanya,U.N., Li,Y., Zhang,J.F., Garcia-Barrio,M.T., Rudolph,T.K., Rudolph,V., Bonacci,G., Baker,P.R.S., Xu,H.E., Batthyany,C.I., Chen,Y.E., Hallis,T.M., Freeman,B.A. Covalent Peroxisome Proliferator-activated Receptor-gamma Adduction by Nitro-fatty Acids SELECTIVE LIGAND ACTIVITY AND ANTI-DIABETIC SIGNALING ACTIONS. Journal of Biological Chemistry 2010; 285(-16-):12321-12333. >>> Oleic acid; rosiglitazone; oleic acid, nitro-; Mice; 28 days; Controls received mp w/ vehicle; no stress (pg. 12331); animal info (male, C57BL/6J, ob/ob, 8-10 weeks old); functionality of mp verified by plasma agent levels.

Q0056 Strait,K.R., Orkin,J.L., Anderson,D.C., Muly,E.C. Chronic, Constant-Rate, Gastric Drug Infusion in Nontethered Rhesus Macaques (Macaca mulatta). Journal of the American Association for Laboratory Animal Science 2010; 49(-2-):207-214. >>> Antipsychotic; Stomach (gastric lumen); Monkey; 2ML2; 2ML4; 12 months; Controls received mp w/ saline; no stress (see pg. 210); long-term infusion; animal info (male, Rhesus Macaques, 8.12 - 12.18 kg, 5.25 - 7.5 years old); post op. care (buprenorphine, meloxicam); functionality of mp verified by residual volume, plasma drug concentrations; pumps replaced every 2-4 weeks; good methods (pg. 208-210); mp placed SC with a gastric catheter to the stomach; concurrent gastroscopy used confirm catheter tip placement; catheter patency during the year was confirmed by endoscopy; cranial PET scans every 2-4 weeks; agent and saline were alternated during the 52 weeks, as were drug concentrations; "All 5 macaques tolerated placement of the gastric catheter and pump changes well. The animals maintained good condition throughout the course of the yearlong study" (pg. 210); "This method offers an attractive alternative for use in studies that require continuous, constant rate, enteral drug delivery in untethered monkeys. (pg. 214).

Q0429 Wahl,D.R., Petersen,B., Warner,R., Richardson,B.C., Glick,G.D., Opipari,A.W. Characterization of the metabolic phenotype of chronically activated lymphocytes. LUPUS 2010; 19(-13-):1492-1501. >>> Glucose, C13 labeled; Water; SC; Mice; 2001D; 6 hours; Animal info (9 mo old, autoimmune, female, NZB/W, age matched BALB/c, female); functionality of mp verified by plasma glucose levels.

Q0192 Wahl,E.C., Aronson,J., Liu,L.C., Fowlkes,J.L., Thrailkill,K.M., Bunn,R.C., Skinner,R.A., Miller,M.J., Cockrell,G.E., Clark,L.M., Ou,Y., Isales,C.M., Badger,T.M., Ronis,M.J., Sims,J., Lumpkin,CK Jr  Journal of Bone and Mineral Research 2010; 25(-1-):114-123. >>> Tumor necrosis factor, recomb. mouse; PBS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (male, C57BL/6 wt, p21 KO, 3 months old, distraction osteogenesis); functionality of mp verified by TNF serum levels.

P9491 Adachi,M., Autry,A.E., Covington,HE III, Monteggia,L.M. MeCP2-Mediated Transcription Repression in the Basolateral Amygdala May Underlie Heightened Anxiety in a Mouse Model of Rett Syndrome. Journal of Neuroscience 2009; 29(-13-):4218-4227. >>> Hydroxamic acid, suberoylanilide; Cyclodextrin; PBS; CSF/CNS (basolateral amygdala); Mice; 14 days; Controls received mp w/ vehicle; functionality of mp verified by examining the levels of acetylated histone H3 and H4; enzyme inhibitor (histone deacetylase); animal info (male, C57BL/6, 8 wks old); 5% cyclodextrin used; Plastics One bilateral cannula used; suberoylanilide hydroxamic acid also known as SAHA; behavioral testing (rotarod and locomotor activity, open-field test, elevated-plus maze, social interaction test; fear conditioning, pain sensitivity).

P9856 Adkesson,M.J., Citino,S.B., Dennis,P.M., Fiorello,C.V., Lafortune,M., Bennett,R.A. MEDICAL AND SURGICAL MANAGEMENT OF OTITIS IN CAPTIVE BONGO (TRAGELAPHUS EURYCERUS). JOURNAL OF ZOO AND WILDLIFE MEDICINE 2009; 40(-2-):332-343. >>> Amikacin; Ear; Antelope (bongo); 2ML4; 24 days; Functionality of mp verified by residual volume; animal info (10 years old, male, 326 kg); "An osmotic pump was beneficial for antibiotic delivery in case 2 and may be an effective therapy for low-grade infections or for continued therapy once a severe infection is under control." pg. 340.

P9799 Becklund,B.R., Hansen,DW Jr, DeLuca,H.F. Enhancement of 1,25-dihydroxyvitamin D3-mediated suppression of experimental autoimmune encephalomyelitis by calcitonin. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009; 106(-13-):5276-5281. >>> Calcitonin, salmon; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (Fig. 2); animal info (female, C57BL/6, 6 wks old).

Q0562 Bennett,S.P., Boyd,T.D., Norden,M., Padmanabhan,J., Neame,P., Wefes,I., Potter,H. A novel technique for simultaneous bilateral brain infusions in a mouse model of neurodegenerative disease. Journal of Neuroscience Methods 2009; 184(-2-):320-326. >>> Antichymotrypsin, alpha-1; CSF, artificial; CSF/CNS; CSF/CNS (hippocampal parenchyma); Mice; 1004; 28 days; Controls received mp w/ vehicle; animal info (PS/APP, PS/APP/ACT, 10 mo old); functionality of mp verified by residual volume; cyanoacrylate adhesive; multiple pumps per animal (2); stability verified ("minimal degradation" after 28 days); custom catheter solution for simultaneous bilateral brain infusions; nitrile cap used to affix custom catheters to the skull; pump plus custom catheter assembly Fig 3e; "entire catheters and pumps we used can be completely removed without terminating the life of the animal" pg 326.

P9544 Bodell,P.W., Kodesh,E., Haddad,F., Zaldivar,F.P., Cooper,D.M., Adams,G.R. Skeletal muscle growth in young rats is inhibited by chronic exposure to IL-6 but preserved by concurrent voluntary endurance exercise. Journal of Applied Physiology 2009; 106(-2-):443-453. >>> Interleukin-6, recomb. rat; IM (gastrocnemius); Rat; 2002; 14 days; Controls received mp w/vehicle; functionality of mp verified by residual volume; animal info (Sprague Dawley, 5.5 wks old, 118g); fenestrated catheter used.

P9393 Bridges,J.P., Gilbert,J.S., Colson,D., Gilbert,S.A., Dukes,M.P., Ryan,M.J., Granger,J.P. Oxidative Stress Contributes to Soluble Fms-Like Tyrosine Kinase-1 Induced Vascular Dysfunction in Pregnant Rats. American Journal of Hypertension 2009; 22(-5-):564-568. >>> Flt-1, soluble; Saline, sterile; IP; Rat (pregnant); 2001; 5 days; Controls received mp w/ vehicle; functionality of mp verified by sFlt-1 plasma/serum concentrations; cardiovascular; animal info (female, Sprague Dawley, gd13).

P9753 Buckmaster,P.S., Ingram,E.A., Wen,X.L. Inhibition of the Mammalian Target of Rapamycin Signaling Pathway Suppresses Dentate Granule Cell Axon Sprouting in a Rodent Model of Temporal Lobe Epilepsy. Journal of Neuroscience 2009; 29(-25-):8259-8269. >>> Rapamycin; DMSO; ethanol; fluorescein; CSF/CNS (dentate gyrus); Rat; 2004; 1-2 months; Controls received mp w/vehicle; functionality of mp verified by fluorescein labeling; long-term study; pumps replaced after 1 month; ALZET brain infusion kit 2 used; animal info (male, Sprague Dawley, 34 days old); 50% DMSO used; 15% ethanol used.

Q0266 Byrnes,K.R., Stoica,B., Riccio,A., Pajoohesh-Ganji,A., Loane,D.J., Faden,A.I. Activation of Metabotropic Glutamate Receptor 5 Improves Recovery after Spinal Cord Injury in Rodents. Annals of Neurology 2009; 66(-1-):63-74. >>> Hydroxyphenylglycine, (RS)-2-chloro-5; DMSO; saline; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (adult, male, Sprague-Dawley, 275-325 g); functionality of mp verified by pump weight; spinal cord injury; 10% DMSO used; compound also known as CHPG; CHPG is a selective mGluR5 agonist.

Q0269 Cain,S.M., Ruest,T., Pimlott,S., Patterson,J., Duncan,R., Dewar,D., Sills,G.J. High resolution micro-SPECT scanning in rats using 125I beta-CIT: Effects of chronic treatment with carbamazepine. Epilepsia 2009; 50(-8-):1962-1970. >>> Carbamazepine; DMSO; propylene glycol; ethyl alcohol; acetone; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (adult, male, Sprague-Dawley, 160-270 g); functionality of mp verified by serum drug levels; 42% DMSO used; identified 3 mg/kg/day as the highest dose that could be reliably administered via minipumps over a 14-day period at 37 degrees Celsius, pg. 1969.

P9395 Carlstrom,M., Lai,E.Y., Ma,Z., Patzak,A., Brown,R.D., Persson,A.E.G. Role of NOX2 in the regulation of afferent arteriole responsiveness. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 296(-1-):R72-R79. >>> Angiotensin II; SC; Mice; 14 days; Functionality of mp verified by blood pressure; no stress (see pg. R73); cardiovascular; peptides; animal info (male, C57BL/6J, wt NOX2 -/-, 20-25 g.).

P9746 Catania,C., Sotiropoulos,I., Silva,R., Onofri,C., Breen,K.C., Sousa,N., Almeida,O.F.X. The amyloidogenic potential and behavioral correlates of stress. Molecular Psychiatry 2009; ;(-1-):95-105. >>> Amyloid protein, beta (1-40); Water, distilled; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/vehicle; functionality of mp verified; ALZET brain infusion kit used; peptides; animal info (male, Wistar, 14 months old); neurodegenerative (Alzheimer's disease); cannula patency verified.

Q0464 Chen,S.R., Samoriski,G., Pan,H.L. Antinociceptive effects of chronic administration of uncompetitive NMDA receptor antagonists in a rat model of diabetic neuropathic pain. Neuropharmacology 2009; 57(-2-):121-126. >>> Neramexane mesylate; memantine; gabapentin; Saline, sterile; water; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; animal info (male, 225-250 g, STZ induced diabetes); functionality of mp verified by plasma drug levels; post op. care (penicillin); wound clips used; neuropathic pain.

Q0631 Coccurello,R., Brina,D., Caprioli,A., Conti,R., Ghirardi,O., Schepis,F., Moles,A. 30 Days of Continuous Olanzapine Infusion Determines Energy Imbalance, Glucose Intolerance, Insulin Resistance, and Dyslipidemia in Mice. Journal of Clinical Psychopharmacology 2009; 29(-6-):576-583. >>> Olanzapine; Acetic acid; saline, isotonic; sodium hydroxide; SC; Mice; 2004; 30 days; Controls received mp w/ vehicle; animal info (CD-1, 11 wks old, female); functionality of mp verified by residual volume; "Although (degradation of AAP agents in mini-pump reservoir over time) is a serious concern, significant increases of body weight and food intake were found in female rats33Y35 and mice14 under constant OL infusion via implanted mini-pumps" pg 582.

Q0038 Cooke,J.H., Patterson,M., Patel,S.R., Smith,K.L., Ghatei,M.A., Bloom,S.R., Murphy,K.G. Peripheral and Central Administration of Xenin and Neurotensin Suppress Food Intake in Rodents. Obesity 2009; 17(-6-):1135-1143. >>> Xenin; neurotensin; SC; Mice; 1007D; 6 days; Controls received mp w/ saline; peptides; no stress (pg 1142); animal info (male, C57BL/6); comparison of IP injections vs. SC mp; dose-response (table 1); functionality of mp verified by residual volume.

P9404 Doller,A., Gauer,S., Sobkowiak,E., Geiger,H., Pfeilschifter,J., Eberhardt,W. Angiotensin II Induces Renal Plasminogen Activator Inhibitor-1 and Cyclooxygenase-2 Expression Post-Transcriptionally via Activation of the mRNA-Stabilizing Factor Human-Antigen R. American Journal of Pathology 2009; 174(-4-):1252-1263. >>> Angiotensin II; Saline; SC; Rat; 2001; 7, 14 days; 6 hours; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 180-200 g.); nephrology.

Q0446 Flandin,P., Lehr,L., Asensio,C., Giacobino,J.P., Rohner-Jeanrenaud,F., Muzzin,P., Jimenez,M. Uncoupling protein-3 as a molecular determinant of the action of 3,5,3'-triiodothyronine on energy metabolism. Endocrine 2009; 36(-2-):246-254. >>> Triiodothyronine; NaCl; SC; Mice; 2001; 3 days; Controls received mp w/ vehicle; animal info (WT, UCP3KO, 2 mo old, female); functionality of mp verified by "increase in T3 during the treatment".

Q0367 Fuller,D.D., Dougherty,B.J., Sandhu,M.S., Doperalski,N.J., Reynolds,C.R., Hayward,L.F. Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2009; 169(-3-):333-337. >>> Nicotine; SC; Rat (pregnant); 2ML4; 1, 2 weeks; Controls received mp w/ saline; animal info (Sprague-Dawley, female); functionality of mp verified by plasma nicotine levels; post op. care (buprenorphine, carprofen); wound clips used.

Q0265 Gadjanski,I., Boretius,S., Williams,S.K., Lingor,P., Knoeferle,J., Saettler,M.B., Fairless,R., Hochmeister,S., Suehs,K.W., Michaelis,T., Frahm,J., Storch,M.K., Baehr,M., Diem,R. Role of N-Type Voltage-Dependent Calcium Channels in Autoimmune Optic Neuritis. Annals of Neurology 2009; 66(-1-):81-93. >>> Conotoxin, w-; CSF, artificial; CSF/CNS; Rat; 2004; 8, 23 days; Controls received mp w/ vehicle; animal info (female brown Norway, 8-10 wks old); functionality of mp verified by residual volume; w-conotixin is an N-type specific blocker.

P9483 Galeote,L., Berrendero,F., Bura,S.A., Zimmer,A., Maldonado,R. Prodynorphin gene disruption increases the sensitivity to nicotine self-administration in mice. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY 2009; 12(-5-):615-625. >>> Nicotine hydrogen tartrate salt; SC; Mice; 2001; 6 days; Controls received mp w/ saline; functionality of mp verified by residual volume; dependence; animal info (male, C57BL/6, wt, prodynorphin KO) dose (~ 25 mg/kg/d).

Q0603 Gallego,J.M., Ortiz,L., Gutierrez,R., Barcia,J.A. Continuous bilateral infusion of GABA in the dorsomedian nucleus of the thalamus elevates the generalized seizure threshold in amygdala-kindled rats. SEIZURE-EUROPEAN JOURNAL OF EPILEPSY 2009; 18(-7-):537-540. >>> Aminobutyric acid, gamma; Saline; CSF/CNS (dorsomedian nuclei of the thalamus); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (adult, male, Wistar); functionality of mp verified by residual volume; multiple pumps per animal (2); bilateral infusion.

P9407 Giachini,F.R.C., Zemse,S.M., Carneiro,F.S., Lima,V.V., Carneiro,Z.N., Callera,G.E., Ergul,A., Webb,R.C., Tostes,R.C. Interleukin-10 attenuates vascular responses to endothelin-1 via effects on ERK1/2-dependent pathway. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-2-):H489-H496. >>> Tumor necrosis factor-a; Saline; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum TNFa levels; cardiovascular; animal info (male, C57BL/6, wt, IL-1D -/-, 10 wks old).

P9410 Hasegawa,T., Iwanaga,K., Hultquist,D.E., Liao,H., Visovatti,S.H., Pinsky,D.J. Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-4-):H1007-H1016. >>> Riboflavin; Saline; IP; Mice; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by plasma riboflavin levels; dose-response (fig. 1); cardiovascular; immunology; animal info (male, CBA/J, B1OA, 8-12 wks old, cardiac transplantation); antioxidant.

Q0951 Hashimoto,M., Hossain,S., Tanabe,Y., Kawashima,A., Harada,T., Yano,T., Mizuguchi,K., Shido,O. The protective effect of dietary eicosapentaenoic acid against impairment of spatial cognition learning ability in rats infused with amyloid beta(1-40). JOURNAL OF NUTRITIONAL BIOCHEMISTRY 2009; 20(-12-):965-973. >>> Amyloid protein, beta (1-40); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (Wistar, 5 wks old); functionality of mp and cannula placement verified by examining brain slices; neurodegenerative (Alzheimer's disease).

P9869 Herrmann,M., Henneicke,H., Street,J., Modzelewski,J., Kalak,R., Buttgereit,F., Dunstan,C.R., Zhou,H., Seibel,M.J. The challenge of continuous exogenous glucocorticoid administration in mice. Steroids 2009; 74(-2-):245-249. >>> Corticosterone; PEG 400; DMSO; SC; Mice; 2004; 21 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; comparison of SC injections vs. pellet vs SC mp; animal info (Swiss White, male, CD1. 7-9 wks old).

Q0545 Hicks,A.U., Lappalainen,R.S., Narkilahti,S., Suuronen,R., Corbett,D., Sivenius,J., Hovatta,O., Jolkkonen,J. Transplantation of human embryonic stem cell-derived neural precursor cells and enriched environment after cortical stroke in rats: cell survival and functional recovery. European Journal of Neuroscience 2009; 29(-3-):562-574. >>> Cyclosporine A; PEG 400; SC; Rat; 2ML4; 2002; ; Controls received mp w/ vehicle; animal info (male, Wistar, 275-300 g, dMCAO); functionality of mp verified by residual volume; behavioral testing (Montoya's staircase test).

Q0827 Huang,L.H., Haylor,J.L., Hau,Z., Jones,R.A., Vickers,M.E., Wagner,B., Griffin,M., Saint,R.E., Coutts,I.G.C., El Nahas,A.M., Johnson,T.S. Transglutaminase inhibition ameliorates experimental diabetic nephropathy. Kidney International 2009; 76(-4-):383-394. >>> NTU281, dansyl labeled; PBS; Intrarenal; Rat; 2ML4; 1, 4, 8 months; Controls received mp w/ vehicle; animal info (male, Wistar, Han, uninephrectomized); functionality of mp verified by residual volume, Fig 2a; long-term study; pumps replaced every 28 days; enzyme inhibitor (transglutaminase); "cannula was secured using silk ties and tissue glue and run through the muscle wall to the pump located on the scruff" pg 392.

Q0590 Ingram,E.A., Toyoda,I., Wen,X.L., Buckmaster,P.S. Prolonged infusion of inhibitors of calcineurin or L-type calcium channels does not block mossy fiber sprouting in a model of temporal lobe epilepsy. Epilepsia 2009; 50(-1-):56-64. >>> Nicardipine; FK-506; cyclosporin A; DMSO; ethanol; fluorescein; CSF/CNS (dorsal left dentate gyrus); Rat; 2004; 28 days; Controls were treated identically without status epilepticus; animal info (34-52 day old, male, Sprague-Dawley, status epilepticus); functionality of mp verified by fluorescein labeling; ALZET brain infusion kit 2 used; 50% DMSO used; 15% ethanol used.

P9416 Isa,K., Garcia-Espinosa,M.A., Arnold,A.C., Pirro,N.T., Tommasi,E.N., Ganten,D., Chappell,M.C., Ferrario,C.M., Diz,D.I. Chronic immunoneutralization of brain angiotensin-(1-12) lowers blood pressure in transgenic (mRen2) 27 hypertensive rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-1-):R111-R115. >>> Antibody, rabbit anti-rat ANG (1-12); immunoglobulin G, rabbit; CSF, artificial; CSF/CNS; Rat (transgenic); 2ML2; 14 days; Controls received mp w/ IgG; functionality of mp verified by residual volume; no stress (see pg. R111, R112); stability verified by activity of agent in residual volume, 91-98% on day 13; antihypertensive; ALZET brain infusion 2 kit; animal info (male, (mRen2)27 Tg, 22-28 wks old, 550g.); cannula placement confirmed at end of each study.

P9790 Joosen,M.J.A., Jousma,E., van den Boom,T.M., Kuijpers,W.C., Smit,A.B., Lucassen,P.J., Van Helden,H.P.M. Long-term cognitive deficits accompanied by reduced neurogenesis after soman poisoning. Neurotoxicology 2009; 30(-1-):72-80. >>> Olanzapine; Acetic acid, glacial; SC; Rat; 2ML4; 4 weeks; Functionality of mp verified by residual volume; animal info (male, Sprague Dawley); stability verified for 4 weeks by electrospray MS/MS.

P9786 Kanemaru,K., Nishi,K., Hasegawa,S., Diksic,M. Chronic citalopram treatment elevates serotonin synthesis in flinders sensitive and flinders resistant lines of rats, with no significant effect on Sprague-Dawley rats. NEUROCHEMISTRY INTERNATIONAL 2009; 54(-5-6-):363-371. >>> Citalopram hydrobromide; Saline; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; animal info (male, Sprague Dawley, 200 g.).

Q0613 Kawasaki,M., Ponzio,T.A., Yue,C.M., Fields,R.L., Gainer,H. Neurotransmitter regulation of c-fos and vasopressin gene expression in the rat supraoptic nucleus. Experimental Neurology 2009; 219(-1-):212-222. >>> TTX; CNQX; APV; Saline; CSF/CNS (supraoptic nucleus); Rat; 2001D; 3.5 hours; Controls received mp w/ vehicle; animal info (adult, male, Spraque-Dawley, 250-350 g); functionality of mp verified by measuring Fluorogold in the brain; multiple pumps per animal (2); multiple cannulae (2, 28 gauge) used; pumps not implanted but activated by immersion in 0.9% saline at 37°C.; PE-60 tubing used.

Q0588 Keiner,S., Witte,O.W., Redecker,C. Immunocytochemical Detection of Newly Generated Neurons in the Perilesional Area of Cortical Infarcts After Intraventricular Application of Brain-Derived Neurotrophic Factor. Journal of Neuropathology and Experimental Neurology 2009; 68(-1-):83-93. >>> Brain-derived neurotrophic factor; NaCl; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (male, Wistar, 12-14 weeks, 250-300 g); functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; ischemia (focal cortical infarct); behavioral testing (the limb-use asymmetry; tapered beam walking; ladder rung walking); fig. 1c diagram of a parasagittal section of the rat brain showing cannula placement.

P9774 Khan,N., Mupparaju,S.P., Hou,H., Lariviere,J.P., Demidenko,E., Swartz,H., Eastman,A. Radiotherapy in Conjunction with 7-Hydroxystaurosporine: A Multimodal Approach with Tumor pO2 as a Potential Marker of Therapeutic Response. RADIATION RESEARCH 2009; 172(--):592-597. >>> UCN-01; Sodium acetate; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma UCN-01 concentrations; cancer (radiation therapy); animal info (female, C3H/HEJ, 18-20 g.).

Q0775 Khasar,S.G., Dina,O.A., Green,P.G., Levine,J.D. Sound Stress-Induced Long-Term Enhancement of Mechanical Hyperalgesia in Rats Is Maintained by Sympathoadrenal Catecholamines. JOURNAL OF PAIN 2009; 10(-10-):1073-1077. >>> Epinephrine; SC; Rat; 2004; 14 days; Animal info (adrenal medullecomty, adult, male, Sprague Dawley, 300-400 g); functionality of mp verified by plasma drug levels.

Q0439 Lubel,J.S., Herath,C.B., Tchongue,J., Grace,J., Jia,Z.Y., Spencer,K., Casley,D., Crowley,P., Sievert,W., Burrell,L.M., Angus,P.W. Angiotensin-(1-7), an alternative metabolite of the renin-angiotensin system, is up-regulated in human liver disease and has antifibrotic activity in the bile-duct-ligated rat. Clinical Science 2009; 117(-11-12-):375-386. >>> Angiotensin (1-7); Saline; IP; Rat; 2 weeks; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 6 wks old, male); functionality of mp verified by residual volume; peptides.

P9613 Ma,T., Ortiz,S.G., Huang,Z.N., Ren,P.G., Smith,R.L., Goodman,S.B. In Vivo Murine Model of Continuous Intramedullary Infusion of Particles-A Preliminary Study. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2009; 88B(-1-):250-253. >>> Polystyrene particles, blue dyed; PBS; Bone (femur); Mice; 2004; 4 weeks; Functionality of mp verified by gross inspection of retrieved femora; animal info (9 wks old, male, C57BL/6); Fig. 1, X-ray image of pump and delivery system; tissue perfusion (femur).

P9857 Magge,S.N., Malik,S.Z., Royo,N.C., Chen,H.I., Yu,L., Snyder,E.Y., O'Rourke,D.M., Watson,D.J. Role of Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) in Migration of Neural Progenitor Cells Toward Glial Tumors. Journal of Neuroscience Research 2009; 87(-7-):1547-1555. >>> MCP-1, recomb. rat; PBS; BSA; CSF/CNS (corpus callosum); Rat; 1007D; 7 days; Controls received MCP-1 w/o transplant or transplant w/o MCP-1; functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; animal info (adult, Fischer, female, 150-200 g.); brain tissue distribution.

Q0687 Molina-Luna,K., Pekanovic,A., Roehrich,S., Hertler,B., Schubring-Giese,M., Rioult-Pedotti,M.S., Luft,A.R. Dopamine in Motor Cortex Is Necessary for Skill Learning and Synaptic Plasticity. PLoS One 2009; 4(-9-):U127-U136. >>> Levodopa; NaCl; CSF/CNS (primary motor cortex); Rat; ; Controls received mp w/ vehicle; animal info (adult, male, Long-Evans, 332.1 g, ); functionality of mp verified by Nissl staining histology; tissue perfusion (motor cortex).

Q0701 Mouret,A., Lepousez,G., Gras,J., Gabellec,M.M., Lledo,P.M. Turnover of Newborn Olfactory Bulb Neurons Optimizes Olfaction. Journal of Neuroscience 2009; 29(-39-):12302-12314. >>> zVAD; Saline; DMSO; CSF/CNS (olfactory bulb); Mice; 1002; 15 days; Controls received mp w/ vehicle; animal info (2 mo old, male, C57BL/6J); peptides; functionality of mp verified by pump weight; ALZET brain infusion kit 3 used; 1.5% DMSO used; Chicago blue dye used to verify cannula placement.

Q0456 Naghshin,J., McGaffin,K.R., Witham,W.G., Mathier,M.A., Romano,L.C., Smith,S.H., Janczewski,A.M., Kirk,J.A., Shroff,S.G., O'Donnell,C.P. Chronic intermittent hypoxia increases left ventricular contractility in C57BL/6J mice. Journal of Applied Physiology 2009; 107(-3-):787-793. >>> Propranolol; SC; Mice; 4 weeks; Animal info (male, C57BL/6J, 20-25 g, 9-12 wk old); functionality of mp verified by heart rate.

Q0282 Schmidt,C., Bezuidenhout,D., Beck,M., Van der Merwe,E., Zilla,P., Davies,N. Rapid three-dimensional quantification of VEGF-induced scaffold neovascularisation by microcomputed tomography. Biomaterials 2009; 30(-30-):5959-5968. >>> Vascular endothelial growth factor (165); SC; Rat; 1002; 10 days; Controls received mp w/ PBS; animal info (male, Wistar, 234-254 g); functionality of mp verified by residual volume; post op. care (buprenorphine); pump connected to a porous polyurethane construct, schematic on Figure 1A;.

P9444 Shao,W.J., Seth,D.M., Navar,L.G. Augmentation of endogenous intrarenal angiotensin II levels in Val5-ANG II-infused rats. American Journal of Physiology-Renal Physiology 2009; 296(-5-):F1067-F1071. >>> Angiotensin II, val5-; angiotensin II, Ile5; SC; Rat; 2002; 13 days; Controls received sham operation; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 240-270 g.); nephrology.

P9852 Simcock,J.W., Penington,A.J., Morrison,W.A., Thompson,E.W., Mitchell,G.M. Endothelial Precursor Cells Home to a Vascularized Tissue Engineering Chamber by Application of the Angiogenic Chemokine CXCL12. Tissue Engineering Part A 2009; 15(-3-):655-664. >>> CXCL12; stromal cell-derived factor-1a, recomb. human; BSA; IV (arteriorvenous loop); Rat (nude); 1007D; 7 days; Functionality of mp verified; animal info (CBH/R nu/nu, 200-250 g.); diagram illustrating relationship of pump to chamber construct, fig. 1.

Q0264 Sykes,J.M., Cox,S., Ramsay,E.C. Evaluation of an osmotic pump for fentanyl administration in cats as a model for nondomestic felids. American Journal of Veterinary Research 2009; 70(-8-):950-955. >>> Fentanyl; Saline, sterile; SC; Cat (felid); 2ML1; 96 hours; Animal info (spayed, female, 56 months old, 4.5 kg); functionality of mp verified by residual volume; comparison of transdermal patch vs. SC mp; no stress see pg 952; "if fentanyl is "to be used for nondomestic cats, then the faster elimination of fentanyl after removal of a delivery device is an important advantage of the osmotic pump versus the transdermal patch" pg 953; "Compared with a transdermal patch, an osmotic pump provides several pharmacokinetic advantages for fentanyl administration in cats." pg 954.

Q0083 Tang,G.B., Cui,J.G., Wang,D.H. Role of hypoleptinemia during cold adaptation in Brandt's voles (Lasiopodomys brandtii). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-5-):R1293-R1301. >>> Leptin, recomb. murine; PBS; SC; Prairie vole; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, Brandts, 90-120 days old); functionality of mp verified by serum leptin levels.

P9867 Thakker,D.R., Weatherspoon,M.R., Harrison,J., Keene,T.E., Lane,D.S., Kaemmerer,W.F., Stewart,G.R., Shafer,L.L. Intracerebroventricular amyloid-beta antibodies reduce cerebral amyloid angiopathy and associated micro-hemorrhages in aged Tg2576 mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009; 106(-11-):4501-4506. >>> Immunogloculin G1; 6E10; CSF/CNS; Mice; 2004; ; Functionality of mp verified in vitro for 5 weeks; stability verified in vitro for 5 weeks; animal info (male, 16-18 months old, Tg2576); neurodegenerative (Alzheimer's Disease); 6E10 is a mouse monoclonal IgG1; brain tissue distribution.

P9709 Thompson,E.L., Amber,V., Stamp,G.W.H., Patterson,M., Curtis,A.E., Cooke,J.H., Appleby,G.F., Dhillo,W.S., Ghatei,M.A., Bloom,S.R., Murphy,K.G. Kisspeptin-54 at high doses acutely induces testicular degeneration in adult male rats via central mechanisms. British Journal of Pharmacology 2009; 156(-4-):609-625. >>> Kisspeptin-54; Saline; SC; CSF/CNS; Rat; 2001D; 2001; 1, 2, 3 days; 6, 12, 24 hours; Controls received mp w/vehicle; functionality of mp verified; peptides; animal info (adult, male, Wistar, 275-325 g.).

Q0767 Traub,M.L., De Butte-Smith,M., Zukin,R.S., Etgen,A.M. Oestradiol and Insulin-Like Growth Factor-1 Reduce Cell Loss after Global Ischaemia in Middle-Aged Female Rats. Journal of Neuroendocrinology 2009; 21(-12-):1038-1044. >>> Insulin-like growth factor-I; Saline, sterile; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 9-11 mo old, adult, 300-500 g); functionality of mp verified by residual volume; ALZET brain infusion kit used; cannula placement verified by inspection of cryostat sectioning; neuroprotection.

Q0659 Tsai,Y.J., Lin,C.T., Huang,C.T., Wang,H.Y., Tien,L.T., Chen,S.H., Lue,J.H. Neuropeptide Y Modulates c-Fos Protein Expression in the Cuneate Nucleus and Contributes to Mechanical Hypersensitivity following Rat Median Nerve Injury. Journal of Neurotrauma 2009; 26(-9-):1609-1621. >>> Neuropeptide Y, neuropeptide y antagonist; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 180-250 g); functionality of mp verified by residual volume.

P9868 Virk,M.S., Arttamangkul,S., Birdsong,W.T., Williams,J.T. Buprenorphine Is a Weak Partial Agonist That Inhibits Opioid Receptor Desensitization. Journal of Neuroscience 2009; 29(-22-):7341-7348. >>> Buprenorphine; DMSO; water; Rat; 2ML1; ; Controls received mp w/ vehicle; functionality of mp verified by plasma drug levels; animal info (male, Sprague Dawley, 150-200 g.); 40% DMSO used; "the osmotic minipump delivered buprenorphine efficiently and predictably" pg. 7342.

Q0747 Wang,W.F., Baghdoyan,H.A., Lydic,R. Leptin Replacement Restores Supraspinal Cholinergic Antinociception in Leptin-Deficient Obese Mice. JOURNAL OF PAIN 2009; 10(-8-):836-843. >>> Leptin; Mice; 1007D; 7 days; Animal info (adult, male, B6, obese); functionality of mp verified by leptin ELISA kit; obesity.

P9460 Wells,S.M., Buford,M.C., Migliaccio,C.T., Holian,A. Elevated Asymmetric Dimethylarginine Alters Lung Function and Induces Collagen Deposition in Mice. American Journal of Respiratory Cell and Molecular Biology 2009; 40(-2-):179-188. >>> Arginine, asymmetric dimethyl-; SC; Mice; 2 weeks; Controls received mp w/ saline; functionality of mp verified by serum ADMA levels; dose-response (table 1); enzyme inhibitor (nitric oxide synthase); animal info (Balb/c); pulmonology.

P9659 Wilson-Gerwing,T.D., Johnston,J.M., Verge,V.M.K. p75 Neurotrophin Receptor Is Implicated in the Ability of Neurotrophin-3 to Negatively Modulate Activated ERK1/2 Signaling in TrkA-Expressing Adult Sensory Neurons. Journal of Comparative Neurology 2009; 516(-1-):49-58. >>> Neurotrophin 3; oligonucleotides, antisense; oligonucleotides, sense; PBS; Streptomycin; albumin, rat serum; penicillin; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/vehicle; functionality of mp verified by flurorescence; antisense (P75); animal info (young male, wistar, 250-300.

P9516 Wissman,A.M., Brenowitz,E.A. The Role of Neurotrophins in the Seasonal-Like Growth of the Avian Song Control System. Journal of Neuroscience 2009; 29(-20-):6461-6471. >>> TrkB-Fc; TrkC-Fc; brain-derived neurotrophic factor; nerve growth factor; PBS; CSF/CNS (robust nucleus of the arcopallium); Bird; 1002; ; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; animal info (sparrow, adult, male, Gambel's white crowned).

Q0619 Witzenrath,M., Gutbier,B., Schmeck,B., Tenor,H., Seybold,J., Kuelzer,R., Grentzmann,G., Hatzelmann,A., van Laak,V., Tschernig,T., Mitchell,T.J., Schudt,C., Rosseau,S., Suttorp,N., Schuette,H. Phosphodiesterase 2 inhibition diminished acute lung injury in murine pneumococcal pneumonia. Critical Care Medicine 2009; 37(-2-):584-590. >>> PDP, hydroxy; SC; Mice; 2001; ; Controls received mp w/ vehicle; animal info (female, Balb/C, C57Bl/6, 8-12 wks old); functionality of mp verified by plasma drug levels; enzyme inhibitor (phosphodiesterase-2); PDP also known as 9-(6-phenyl-2-oxohex-3-yl)-2-(3,4-dimethoxybenzyl)- purin-6one; "PDE2 inhibition by continuous infusion of hydroxy-PDP was capable of extensively reducing microvascular leakage in pneumonic mice without altering PDE2 mRNA expression. " pg 589.

P9577 Wright,J.W., Meighan,P.C., Brown,T.E., Wiediger,R.V., Sorg,B.A., Harding,J.W. Habituation-induced neural plasticity in the hippocampus and prefrontal cortex mediated by MMP-3. Behavioural Brain Research 2009; 203(-1-):27-34. >>> MMP-3i; CSF/CNS; Rat; 2001; 6 days; Controls received mp aCSF; functionality of mp verified by residual volume; animal info (male, Sprague Dawley, 300-350g); behavioral testing (head-shake response habituation); cannula placement verified w/icv injection of fast green dye; enzyme inhibitor (metalloproteinase-3).

P9470 Ying,Z.K., Yue,P.B., Xu,X.H., Zhong,M.H., Sun,Q.H., Mikolaj,M., Wang,A.X., Brook,R.D., Chen,L.C., Rajagopalan,S. Air pollution and cardiac remodeling: a role for RhoA/Rho-kinase. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1540-H1550. >>> Angiotensin II; Saline; acetic acid; SC; Mice; 14 days; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, C57BL/6, 8 wks old); pulmonology.

P9968 Zhang,Q., Yao,F.R., O'Rourke,S.T., Qian,S.Y., Sun,C.W. Angiotensin II enhances GABAB receptor-mediated responses and expression in nucleus tractus solitarii of rats. American Journal of Physiology-Heart and Circulatory Physiology 2009; 297(-5-):H1837-H1844. >>> Angiotensin II; CSF/CNS; Rat; 2004; 7 days; Controls received mp w/ aCSF; peptides; cardiovascular; ALZET brain infusion kit 2 used; animal info (male, Sprague-Dawley, 290-350 g); functionality of mp verified by mean arterial BP.

P9767 Zhang,Q., Chen,C., Lue,J.G., Xie,M.J., Pan,D.J., Luo,X., Yu,Z.Y., Dong,Q., Wang,W. Cell Cycle Inhibition Attenuates Microglial Proliferation and Production of IL-1-beta, MIP-1-alpha, and NO After Focal Cerebral Ischemia in the Rat. Glia 2009; 57(-8-):908-920. >>> Roscovitine; DMSO; saline; CSF/CNS; Rat; 2001; ; Controls received mp w/vehicle; functionality of mp verified by "weighing the cassette before placement and after the experiment" pg. 909; enzyme inhibitor (cyclin dependant kinase); ALZET brain infusion kit used; ischemia (cerebral); animal info (adult, male, Sprague Dawley, 220-270 g); 0.2% DMSO used; MCAO.

P9574 Zhao,R., Raub,T.J., Sawada,G.A., Kasper,S.C., Bacon,J.A., Bridges,A.S., Pollack,G.M. Breast Cancer Resistance Protein Interacts with Various Compounds in Vitro, but Plays a Minor Role in Substrate Efflux at the Blood-Brain Barrier. Drug Metabolism and Disposition 2009; 37(-6-):1251-1258. >>> Cimetidine; alfuzosin; dipyridamole; DMSO; SC; Mice; 2001D; 24 hours; Functionality of mp verified by residual volume; cancer (breast); animal info (Mdrla KO, wt, abcg2 -/-); 50%, 80% DMSO used; "(with dipyridamole in 80% DMSO)" precipitation was visible around the exit hole at the end of the experiment" pg. 1255.

P8929 Badlani,N., Inoue,A., Healey,R., Coutts,R., Amiel,D. The protective effect of OP-1 on articular cartilage in the development of osteoarthritis. OSTEOARTHRITIS AND CARTILAGE 2008; 16(-5-):600-606. >>> Osteogenic protein-1; Lactose; Knee (articular cavity); Rabbit; 2004; 6 weeks; Controls received mp w/ vehicle; functionality of mp verified by separate in vivo test; peptides; post op. care ("appropriate"); animal info (New Zealand, White, 1 yr old); also known as BMP-7 or bone morphogenic protein-7; catheter was secured with polyethylene stents; "The efficacy of the (ALZET Pump) was tested in vivo before beginning the study with good results"; "constant infusion of OP-1 may be the most ideal form of delivery"; long-term study; good methods.

P9391 Birsoy,K., Soukas,A., Torrens,J., Ceccarini,G., Montez,J., Maffei,M., Cohen,P., Fayzikhodjaeva,G., Viale,A., Socci,N.D., Friedman,J.M. Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-35-):12985-12990. >>> Leptin; PBS; SC; Mice (transgenic); 8 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides; animal info (female, C57BL/6J, BAC Hg, 7-8 wks old).

P9260 Bordia,T., Campos,C., Huang,L.P., Quik,M. Continuous and intermittent nicotine treatment reduces L-3,4-dihydroxyphenylalanine (L-DOPA)-induced dyskinesias in a rat model of Parkinson's disease. Journal of Pharmacology and Experimental Therapeutics 2008; 327(-1-):239-247. >>> Nicotine, free base; Water, sterilized; SC; Rat; 2004; 2006; 8-10 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma continine levels; comparison of PO vs. mp; long-term study pumps replaced after 4 weeks; post op. care (buprenorphine); animal info (male, Sprague Dawley, 6-H DA lesion, 250 g.); neurodegenerative (Parkinson's disease); behavioral testing (forelimb asymmetry test, axial dystonia, orolingual movement, rotational behavior).

R0284 Brunton,P.J., Arunachalam,S., Russell,J.A. CONTROL OF NEUROHYPOPHYSIAL HORMONE SECRETION, BLOOD OSMOLALITY AND VOLUME IN PREGNANCY. Journal of Physiology and Pharmacology 2008; 59(-;-):27-45. >>> Relaxin, recomb. human, synthetic; CSF/CNS; Rat; 2001; 7 days; Review; controls received mp w/ aCSF; animal info (virgin); functionality of mp verified by plasma levels.

Q0037 Clark,S.R., McMahon,C.J., Gueorguieva,I., Rowland,M., Scarth,S., Georgiou,R., Tyrrell,P.J., Hopkins,S.J., Rothwell,N.J. Interleukin-1 receptor antagonist penetrates human brain at experimentally therapeutic concentrations. Journal of Cerebral Blood Flow and Metabolism 2008; 28(-2-):387-394. >>> Interleukin-1 receptor antagonist, recomb. met human; IV (jugular); Rat; 2001D; 24 hours; Controls received mp w/ vehicle; peptides; animal info (male, Sprague-Dawley, MCAO); ischemia (cerebral); functionality of mp verified by CSF and plasma IL-1RA levels.

P8961 Danchuk,S., Sukhanov,S., Horvat,D., Uddin,M.N., Puschett,J.B. Effects of resibufogenin in experimental hypertension. American Journal of Nephrology 2008; 28(-1-):8-13. >>> Angiotensin II; SC; Rat; 2002; 13 days; Controls received sham surgery; functionality of mp verified by systolic blood pressure; replacement therapy (nephrectomy); peptides; animal info (male, CD, 300-350 g.); nephrology.

P9234 Di Zhang,A., Cat,A.N.D., Soukaseum,C., Escoubet,B., Cherfa,A., Messaoudi,S., Delcayre,C., Samuel,J.L., Jaisser,F. Cross-Talk Between Mineralocorticoid and Angiotensin II Signaling for Cardiac Remodeling. Hypertension 2008; 52(-6-):1060-1U44. >>> Angiotensin II; SC; Mice (transgenic); 2 months; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure); long-term study; pumps replaced; cardiovascular; peptides; animal info (male, MHCtTA/tetO-hMR DTg, 2 months old, 28-35 g.).

P9384 Fife,K.L., Liu,Y.M., Schmelzer,K.R., Tsai,H.J., Kim,I.H., Morisseau,C., Hammock,B.D., Kroetz,D.L. Inhibition of Soluble Epoxide Hydrolase Does Not Protect against Endotoxin-Mediated Hepatic Inflammation. Journal of Pharmacology and Experimental Therapeutics 2008; 327(-3-):707-715. >>> AUDA; AUDA-PEG; AEPU; Cyclodextrin, hydroxypropyl-b-; saline, sterile; SC; Mice; 2001; 6 days; Controls received mp w/ vehicle; functionality of mp verified by blood concentrations; no stress (see pg. 710); half-life (p. 709) "very short"; enzyme inhibitor (epoxide hydrolase); animal info (male, C57BL/6, 6-8 wks old); "Importantly, this study also shows that osmotic pumps are an effective route for administering continuous infusions of precise doses of sEH inhibitors to mice that result in plasma levels well above the IC50 value." (p. 714); 30% cyclodextrin used.

P9237 Galvao,R.P., Garcia-Verdugo,J.M., varez-Buylla,A. Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats. Journal of Neuroscience 2008; 28(-50-):13368-13383. >>> Ara-C; brain-derived neurotrophic factor; Saline; BSA; CSF/CNS; Rat; mice; 1007D; 2002; 6, 14 days; Controls received mp w/ vehicle; functionality of mp verified by weight loss due to BDNF's appetite suppression (p. 13377), residual volume; stability verified by testing activity of residual BDNF volume after 14 days/37 degrees Celsius; brain tissue distribution; peptides; post op. care (Buprenorphine); animal info (male, Sprague Dawley, 250 g., male CD-1 3 mo old); mp/cannula placement visually confirmed after brain sectioning.

P9784 Gehrig,S.M., Ryall,J.G., Schertzer,J.D., Lynch,G.S. Insulin-like growth factor-I analogue protects muscles of dystrophic mdx mice from contraction-mediated damage. Experimental Physiology 2008; 93(-11-):1190-1198. >>> Insulin-like growth factor I, LR-; Saline; HCl; SC; Mice; 1004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; animal info (male, BL/10, mdx, dystrophic, 8-10 wks old).

P8781 Graciano,M.L., Nishiyama,A., Jackson,K., Seth,D.M., Ortiz,R.M., Prieto-Carrasquero,M.C., Kobori,H., Navar,L.G. Purinergic receptors contribute to early mesangial cell transformation and renal vessel hypertrophy during angiotensin II-induced hypertension. American Journal of Physiology-Renal Physiology 2008; 294(-1-):F161-F169. >>> Angiotensin II; SC; Rat; 14 days; Controls received sham surgery; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 200-250g.).

P9313 Hippert,C., Dubois,G., Morin,C., Disson,O., Ibanes,S., Jacquet,C., Schwendener,R., Antignac,C., Kremer,E.J., Kalatzis,V. Gene transfer may be preventive but not curative for a lysosomal transport disorder. MOLECULAR THERAPY 2008; 16(-8-):1372-1381. >>> Cyclosporin A; SC; Mice; 28 days; Controls received no treatment; functionality of mp verified by blood CsA levels; animal info (male, 1295v/C57BL6 Ctns -/-; 3, 5 months old); "A whole blood immunoassay demonstrated that CsA was continually administered over the 28-day period" (p.1375); metabolic disorders.

Q0618 Ivanova,L.N., Lavrinenko,V.A., Babina,A.V., Khegay,I.I. Structure and Concentration Capacity of the Kidneys in Brattleboro Rats under Conditions of Long-Term Vasopressin Treatment. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE 2008; 146(-5-):642-646. >>> Vasopressin, arginine; SC; Rat; 2ML4; 28 days; Controls received empty plastic capsules; animal info (2 months old, male, VP -/-); functionality of mp verified by osmolarity of excreted urine.

P8921 Jacobi,J., Maas,R., Cordasic,N., Koch,K., Schmieder,R.E., Boeger,R.H., Hilgers,K.F. Role of asymmetric dimethylarginine for angiotensin II-induced target organ damage in mice. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-2-):H1058-H1066. >>> Angiotensin II; PBS; SC; Mice (transgenic); 2004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; dose-response (fig. 5); stress/adverse reaction: (see pg. H1060) 3 animals died (1 after 2 wks with no signs of infection or infarction, 2 after 4+6 days with intrathoracic hemorrhage); cardiovascular; peptide; animal info (C57BL/6J, wt, DDAH1, Tg, male, 4 months old).

P8815 Jin,H.K., Yang,R.H., Zheng,Z., Romero,M., Ross,J., Bou-Reslan,H., Carano,R.A.D., Kasman,I., Mai,E., Young,J., Zha,J.P., Zhang,Z.M., Ross,S., Schwall,R., Colbern,G., Merchant,M. MetMAb, the one-armed 5D5 anti-c-Met antibody, inhibits orthotopic pancreatic tumor growth and improves survival. Cancer Research 2008; 68(-11-):4360-4368. >>> Hepatocyte growth factor, human; Dextran sulfate; IP; Mice (nude); 1002; ; Controls received mp w/ vehicle; functionality of mp verified by human HGF serum levels; long-term study; pumps replaced every 12 to 14 days; cancer (pancreatic); peptides; animal info (female, nu/nu).

P9486 Johnson,M.A., Fraley,G.S. Rat RFRP-3 Alters Hypothalamic GHRH Expression and Growth Hormone Secretion but Does Not Affect KiSS-1 Gene Expression or the Onset of Puberty in Male Rats. Neuroendocrinology 2008; 88(-4-):305-315. >>> Oligonucleotide, antisense; oligonucleotide, mismatch; NPFF; RFamide-related peptide-3; PBS; CSF/CNS; CSF/CNS (third ventricle); Rat; 2002; 7, 14 days; Controls received mp w/ vehicle or mismatch ODN; functionality of mp verified; antisense (RFRP-3); ALZET brain infusion kit used; peptides; animal info (male, Sprague Dawley, 24 days old, 60 g., 35 days old); endocrinology; cannula placement confirmed histologically at end of experiment.

P9038 Kawaraguchi,Y., Sasaoka,N., Kawaguchi,M., Inoue,S., Hayashi,H., Konishi,N., Furuya,H. Effect of continuous morphine infusion on hypoxic-ischaemic brain damage of neonatal rats. Acta Anaesthesiologica Scandinavica 2008; 52(-8-):1111-1115. >>> Morphine sulfate; SC; Rat; 1003D; 72 hours; Controls received mp w/ saline; functionality of mp verified by residual volume; dose-response (fig. 1); animal info (Sprague Dawley, 70 days old); pumps primed in sterile saline @ 37 degrees Celsius for 4 hours.

P8766 King,A.J., Novotny,M., Swain,G.M., Fink,G.D. Whole body norepinephrine kinetics in ANG II-salt hypertension in the rat. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 294(-4-):R1262-R1267. >>> Angiotensin II; SC; Rat; 2ML2; 14 days; Functionality of mp verified by mean arterial blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 225-250g.).

P8764 Klieverik,L.P., Sauerwein,H.P., Ackermans,M.T., Boelen,A., Kalsbeek,A., Fliers,E. Effects of thyrotoxicosis and selective hepatic autonomic denervation on hepatic glucose metabolism in rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-3-):E513-E520. >>> Thyroxine, L-; Propylene glycol; NaOH; SC; Rat; 2ML2; 10 days; Functionality of mp verified by T4 plasma concentrations; dose-response (Fig. 1); no stress (see pg. E515); post op. care (Temgesic); animal info (male, Wistar, 325-375g.); hepatic sympathetic or parasympathetic denervation.

P8953 Knedla,A., Riepl,B., Lefevre,S., Kistella,S., Grifka,J., Straub,R.H., Gay,S., Schoelmerich,J., Mueller-Ladner,U., Neumann,E. The therapeutic use of osmotic minipumps in the severe combined immunodeficiency (SCID) mouse model for rheumatoid arthritis. Annals of the Rheumatic Diseases 2008; 68(-1-):124-129. >>> Interleukin-10; interleukin-1 receptor antagonist; Saline; DMSO; SC; Mice (SCID); 2004; 40 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; good methods (p.125); peptides; animal info (female, SCID, 4-5 wks old); Rheumatoid arthritis; pump and technique schematics p. 125; stability (with an excellent description of methods) was verified for 40 days @ 37C; 50% DMSO used; "... the application of proteins via osmotic pumps is an affective tool to evaluate the effects of cytokines and inhibitors in vitro." p. 128.

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P8819 Kudlacz,E., Conklyn,M., Andresen,C., Whitney-Pickett,C., Changelian,P. The JAK-3 inhibitor CP-690550 is a potent anti-inflammatory agent in a murine model of pulmonary eosinophilia. European Journal of Pharmacology 2008; 582(-1-3-):154-161. >>> CP-690550; Water, distilled; ethanol; SC; Mice; 2001; 2002; 3, 14 days; Controls received mp w/ vehicle; functionality of mp verified by plasma agent levels; dose-response (Fig. 6); enzyme inhibitor (Janus Kinase 3 (JAK-3)); animal info (female, Balb/c wt, IL-4 -/-, 4-6 wks old); 5% EtOH used.

P8849 LaCroix,C., Freeling,J., Giles,A., Wess,J., Li,Y.F. Deficiency of M2 muscarinic acetylcholine receptors increases susceptibility of ventricular function to chronic adrenergic stress. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-2-):H810-H820. >>> Isoproterenol; SC; Mice; 1 week; Controls received mp w/ saline; functionality of mp verified by residual volume; comparison of IV injections vs. mp; cardiovascular; animal info (male, 129J1 x CF1, wt, M2-AChR -/-, 2-3 months old, 30g.).

P9211 LaMarca,B., Wallukat,G., Llinas,M., Herse,F., Dechend,R., Granger,J.P. Autoantibodies to the Angiotensin Type I Receptor in Response to Placental Ischemia and Tumor Necrosis Factor-alpha in Pregnant Rats. Hypertension 2008; 52(-6-):1168-1172. >>> Tumor necrosis factor-a; Rat (pregnant); 2002; 5 days; Functionality of mp verified by serum TNFa levels; cardiovascular; peptides; ischemia (placental); animal info (female, Sprague Dawley, gd 14-19).

P9425 Leranth,C., Hajszan,T., Szigeti-Buck,K., Bober,J., MacLusky,N.J. Bisphenol A prevents the synaptogenic response to estradiol in hippocampus and prefrontal cortex of ovariectomized nonhuman primates. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-37-):14187-14191. >>> Bisphenol A; Propylene glycol; SC; Monkey; 2ML4; 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; replacement therapy (ovariectomy); post op. care (buprenorphine, carprofen); animal info (female, African green, 4-5 kg.).

P9244 Liao,T.D., Yang,X.P., Liu,Y.H., Shesely,E.G., Cavasin,M.A., Kuziel,W.A., Pagano,P.J., Carretero,O.A. Role of inflammation in the development of renal damage and dysfunction in angiotensin II-induced hypertension. Hypertension 2008; 52(-2-):256-263. >>> Angiotensin II; Saline; acetic acid; SC; Mice; 2, 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, C57BL/6J, wt, CCR2 -/-, 12-14 wks old).

P8909 Liao,Y.J., Safa,P., Chen,Y.R., Sobel,R.A., Boyden,E.S., Tsien,R.W. Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-7-):2705-2710. >>> Immunoglobulin G, rabbit; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice; 1003D; 3 days; Functionality of mp verified w/ Alexa 488-labeled goat anti-rabbit IgG staining of coronal sections through the cerebellum); ALZET brain infusion kit 3 used; post op. care (buprenorphine); animal info (C57BL/6, > 20 g.); neurodegenerative (cerebellar ataxia); "multiple practice surgeries to infuse a low-molecular weight dye Fast Green confirmed consistent delivery of pump" (pg. 2709); behavioral study.

P9308 Liu,J., Pan,H., Gold,M.S., Derendorf,H., Bruijnzeel,A.W. Effects of fentanyl dose and exposure duration on the affective and somatic signs of fentanyl withdrawal in rats. Neuropharmacology 2008; 55(-5-):812-818. >>> Fentanyl citrate; Saline, physiological; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; functionality of mp verified by plasma fentanyl levels; dose-responses (fig. 1); dependence; animal info (male, Wistar, 300-350 g.).

P8773 Lu,H., Rateri,D.L., Feldman,D.L., Charnigo,RJ Jr, Fukamizu,A., Ishida,J.J., Oesterling,E.G., Cassis,L.A., Daugherty,A. Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice. Journal of Clinical Investigation 2008; 118(-3-):984-993. >>> Aliskiren; PBS; SC; Mice; 2004; 4, 12 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma Aliskiren levels; dose-response (fig. 1); long-term study; pumps replaced every 28 days; no stress (see pg. 985); enzyme inhibitor (Renin); cardiovascular; animal info (male, C57BL/6, Ldlr -/-, 6-8 wks old, 30g.).

P8935 Margineanu,D.G., Matagne,A., Kaminski,R.M., Klitgaard,H. Effects of chronic treatment with levetiracetam on hippocampal field responses after pilocarpine-induced status epilepticus in rats. Brain Research Bulletin 2008; 77(-5-):282-285. >>> Levetiracetam; SC; Rat; 3 weeks; 21 days; Controls received mp w/ vehicle; functionality of mp verified by drug plasma levels; dose-response (table 1, pg. 283); animal info (male, Sprague Dawley, 220-250 g.); epilepsy; "chronic treatment with levetiracetam completely inhibits the development of hippocampal hyperexcitability following pilocarpine-induced (status epilepticus)".

P8752 Marko,S.B., Damon,D.H. VEGF promotes vascular sympathetic innervation. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-6-):H2646-H2652. >>> Antibody, anti-VEGF (164 and 120); immunoglobulin G; PBS; Dye, Evans blue; SC; Rat; 1002; 4, 7, 14, 21 days; Controls received mp w/ control IgG; functionality of mp verified by distribution of dye within surgical area; cardiovascular; animal info (male, Sprague Dawley, denervated femor of artery).

P9280 Mitamura,T., Yamada,A., Hanaoka,K., Asano,M., Niwa,T., Seki,J. Effect of pharmacokinetic profile on the pancreatic toxicity and efficacy of tacrolimus in rats. JOURNAL OF TOXICOLOGICAL SCIENCES 2008; 33(-5-):575-584. >>> FK-506; Propylene glycol; ethanol; castor oil, hydrogenated; IV (femoral); Rat; 2002; 9, 14 days; Controls received mp w/ vehicle; functionality of mp verified by blood drug concentration; dose-response (fig. 4); comparison of bolus IV injections vs. mp; immunology; toxicology; animal info (male, Jcl:SD, 9 wks old: Fischer, WKAtt, 8 wks old; skin allograft); 15% EtOH used; "The sustained blood concentration of tacrolimus (via ALZET) with an AUC similar to that of bolus administration was better tolerated and had the same efficacy...These results indicate that the sustained-release formulation of tacrolimus has the potential to improve the safety of tacrolimus." (p. 583); also known as FK-506.

P9212 Ortiz,S.G., Ma,T., Regula,D., Smith,R.L., Goodman,S.B. Continuous Intramedullary Polymer Particle Infusion Using a Murine Femoral Explant Model. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2008; 87B(-2-):440-446. >>> Polystyrene; polyethylene, high molecular weight; Serum, mouse; Bone (femur); Mice; 2004; 2, 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by blue-dyed polystyrene particles; tissue perfusion (femur); dose-response (fig. 4); no stress (see pg. 443); animal info (male, C57BL/6, 12-16 wks old); mp connected to hollow titanium rod by vinyl catheter; titanium rod inserted into femur ex vivo; femur/pump assemblies placed in sterile flasks at 37 degree for 2, 4 wks; "There were no cases of infection or malfunction of the pumps apparatus over the course of the experiment." (p. 443).

P9304 Parra,G., Quiroz,Y., Salazar,J., Bravo,Y., Pons,H., Chavez,M., Johnson,R.J., Rodriguez-Iturbe,B. Experimental induction of salt-sensitive hypertension is associated with lymphocyte proliferative response to HSP70. Kidney International 2008; 74(-;-):S55-S59. >>> Angiotensin II; SC; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 300-350 g.).

Q0054 Petretto,E., Sarwar,R., Grieve,I., Lu,H., Kumaran,M.K., Muckett,P.J., Mangion,J., Schroen,B., Benson,M., Punjabi,P.P., Prasad,S.K., Pennell,D.J., Kiesewetter,C., Tasheva,E.S., Corpuz,L.M., Webb,M.D., Conrad,G.W., Kurtz,T.W., Kren,V., Fischer,J., Hubner,N., Pinto,Y.M., Pravenec,M., Aitman,T.J., Cook,S.A. Integrated genomic approaches implicate osteoglycin (Ogn) in the regulation of left ventricular mass. NATURE GENETICS 2008; 40(-5-):546-552. >>> Angiotensin II; SC; Mice; 1002; 2 weeks; Peptides; cardiovascular; animal info (male, C57BL/6, Ogn +/-, -/-, 9-17 weeks old); functionality of mp verified by systolic blood pressure.

P9795 Phongsisay,V., Susuki,K., Matsuno,K., Yamahashi,T., Okamoto,S., Funakoshi,K., Hirata,K., Shinoda,M., Yuki,N. Complement inhibitor prevents disruption of sodium channel clusters in a rabbit model of Guillain-Barre syndrome. Journal of Neuroimmunology 2008; 205(-1-2-):101-104. >>> Nafamostat mesilate; DMSO; IV (jugular); Rabbit; 2ML1; 7 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; enzyme inhibitor (serine protease); multiple pumps per animal (2); animal info (White); 50% DMSO used.

P9435 Pitzer,C., Krueger,C., Plaas,C., Kirsch,F., Dittgen,T., Mueller,R., Laage,R., Kastner,S., Suess,S., Spoelgen,R., Henriques,A., Ehrenreich,H., Schaebitz,W.R., Bach,A., Schneider,A. Granulocyte-colony stimulating factor improves outcome in a mouse model of amyotrophic lateral sclerosis. Brain 2008; 131(--):3335-3347. >>> Colony-stimulating factor, G; Sorbitol; sodium acetate buffer; tween 80; SC; Mice (transgenic); 2004; 4, 8 weeks; 4 days; Controls received mp w/ vehicle; functionality of mp verified by G-CSF serum concentration; replacement therapy (sciatic nerve axotomy); long-term study; pumps replaced after 4 weeks; half-life (p. 5) 4 hours in humans and rodents; brain tissue distribution; peptide; animal info (female, C57BL/6, wt, SOD1(G93A)Tg, 11 wks old); neurodegenerative (ALS); behavioral testing (rotarod test, motor performance); 0.004% Tween 80 used.

P8844 Pozdeyev,N., Tosini,G., Li,L., Ali,F., Rozov,S., Lee,R.H., Iuvone,P.M. Dopamine modulates diurnal and circadian rhythms of protein phosphorylation in photoreceptor cells of mouse retina. European Journal of Neuroscience 2008; 27(-10-):2691-2700. >>> L-745,870; Cyclodextrin, 2-hydroxypropyl, beta; SC; Mice; 1003D; 2 days; Controls received mp w/ vehicle; functionality of mp verified by agent levels in retina; animal info (C3H/ft/t); L-745,870 is a dopamine D4 receptor antagonist; 45% cyclodextrin used.

P9252 Qin,Z.Y., Gongora,M.C., Ozumi,K., Itoh,S., Akram,K., Ushio-Fukai,M., Harrison,D.G., Fukai,T. Role of Menkes ATPase in Angiotensin II-Induced Hypertension A Key Modulator for Extracellular Superoxide Dismutase Function. Hypertension 2008; 52(-5-):945-U556. >>> Angiotensin II; norepinephrine; tempol; Saline; SC; Mice; 2002; 14 days; Controls received sham surgery with no mp or empty mp; functionality of mp verified by blood pressure; cardiovascular; peptides; multiple pumps per animal (2); animal info (female, C57BL/6EJ blotchy MNK mut, 8-12 wks old).

P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).

P9771 Roche,J.R., Sheahan,A.J., Chagas,L.M., Blache,D., Berry,D.P., Kay,J.K. Long-Term Infusions of Ghrelin and Obestatin in Early Lactation Dairy Cows. Journal of Dairy Science 2008; 91(-12-):4728-4740. >>> Ghrelin, Dap3-; obestatin; DMSO; SC; Cattle (cow); 2ML4; 8 weeks; Controls were untreated; functionality of mp verified by residual volume; long-term study; pumps replaced after 28 days; animal info (multparous); 50% DMSO used; wound clips used.

P9438 Rocksen,D., Elfsmark,D., Heldestad,V., Wallgren,K., Cassel,G., Nyberg,A.G. An animal model to study health effects during continuous low-dose exposure to the nerve agent VX. Toxicology 2008; 250(-1-):32-38. >>> VX; dye, coomassie blue; Propylene glycol 400; saline; SC; Rat; 2002; 2004; 2, 8 weeks; 26 days; Controls received mp w/ vehicle; functionality of mp verified by coomassie blue dye in saline, absorbance measurements for 4 wks; dose-response (fig. 2); comparison of SC injections vs. mp; long-term study; pumps replaced after 4 wks; no stress (see pg. 37); enzyme inhibitor (ACE).

P9439 Sahu,A. Effects of chronic central leptin infusion on proopiomelanocortin and neurotensin gene expression in the rat hypothalamus. Neuroscience Letters 2008; 440(-2-):125-129. >>> Leptin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 2002; 23 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; pumps replaced after 7 days of mp w/aCSF alone for 16 more days of vehicle or agent; peptides; animal info (male, Sprague Dawley, 250 g.).

P8787 Sharma,U., Rhaleb,N.E., Pokharel,S., Harding,P., Rasoul,S., Peng,H.M., Carretero,O.A. Novel anti-inflammatory mechanisms of N-Acetyl-Ser-Asp-Lys-Pro in hypertension-induced target organ damage. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-3-):H1226-H1232. >>> Angiotensin II; peptide, Ac-SDKP; Saline; acetic acid; SC; Mice; 2004; 8 weeks; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; long-term study; pumps replaced after 4 weeks; cardiovascular; peptides; animal info (male, C57BL6/J, 12-14 wks old).

P9218 Shepherd,R.K., Coco,A., Epp,S.B. Neurotrophins and electrical stimulation for protection and repair of spiral ganglion neurons following sensorineural hearing loss. Hearing Research 2008; 242(-1-2-):100-109. >>> Brain-derived neurotrophic factor, recomb. human; Ringer's solution; albumin, guinea pig; Ear (scala tympani); Guinea-pig; 2004; 4 weeks; Controls received no treatment to contralateral cochlea; functionality of mp verified by residual volume and intact connections; peptides; post op. care (Carprofen, Baytril); tissue perfusion (scala tympani); animal info (pigmented, 400-844 g., kanamycin/furosemide deafened).

P9264 Shigenaga,A., Tamura,K., Wakui,H., Masuda,S., Azuma,K., Tsurumi-Ikeya,Y., Ozawa,M., Mogi,M., Matsuda,M., Uchino,K., Kimura,K., Horiuchi,M., Umemura,S. Effect of olmesartan on tissue expression balance between angiotensin II receptor and its inhibitory binding molecule. Hypertension 2008; 52(-4-):672-678. >>> Angiotensin II; SC; Rat; 2002; 6 days; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; animal info (male, Sprague Dawley, 7 wks old).

P9217 Silva,G.B., Garvin,J.L. Angiotensin II-Dependent Hypertension Increases Na Transport-Related Oxygen Consumption by the Thick Ascending Limb. Hypertension 2008; 52(-6-):1091-1098. >>> Angiotensin II; dye, coomassie blue R250; G86976; tempol; SC; kidney (outer medulla); Rat; 1007D; 4, 7 days; Controls received mp w/ vehicle; tissue perfusion (kidney); functionality of mp verified by residual volume and dye infusion; enzyme inhibitor (PKC a/b); cardiovascular; antihypertensive; peptide; cyanoacrylate adhesive; animal info (Sprague Dawley, 7 wks old, 200-250 g.); catheter fenestrated in one side 10 mm from the tip.

P8838 Singhal,R., Shankar,K., Badger,T.M., Ronis,M.J. Estrogenic status modulates aryl hydrocarbon receptor - mediated hepatic gene expression and carcinogenicity. Carcinogenesis 2008; 29(-2-):227-236. >>> Estradiol; SC; Rat; 2002; 15 days; Controls received mp w/ PEG; functionality of mp verified by serum E2 levels; replacement therapy (ovariectomy); animal info (female, Sprague Dawley, 50 days old).

P8937 Slotkin,T.A., Ryde,I.T., MacKillop,E.A., Bodwell,B.E., Seidler,F.J. Adolescent nicotine administration changes the responses to nicotine given subsequently in adulthood: Adenylyl cyclase cell signaling in brain regions during nicotine administration and withdrawal, and lasting effects. Brain Research Bulletin 2008; 76(-5-):522-530. >>> Nicotine bitartrate; Water; SC; Rat; 1002; 17.5 days; Controls received mp w/ vehicle; functionality of mp verified by plasma nicotine and cotinine levels; animal info (Sprague Dawley).

P9051 Song,B.N., Li,Y.X., Han,D.M. Effects of delayed brain-derived neurotrophic factor application on cochlear pathology and auditory physiology in rats. Chinese Medical Journal 2008; 121(-13-):1189-1196. >>> Brain-derived neurotrophic factor, recomb. human; Albumin, rat; Ringer's solution; Ear (cochlea); Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, albino, Sprague Dawley, 10-12 wks old, 220-250 g.); tissue perfusion (cochlea); functionality of mp verified by residual volume; good methods pg. 1191; pumps were primed in sterile saline at 37 degree Celsius for 48 hours; 1-cm len.

P9854 Stegelmeier,B.L., Molyneux,R.J., Asano,N., Watson,A.A., Nash,R.J. The Comparative Pathology of the Glycosidase Inhibitors Swainsonine, Castanospermine, and Calystegines A3, B2, and C1 in Mice. Toxicologic Pathology 2008; 36(-5-):651-659. >>> Swainsonine; castanospermine; calystegine B2; calystegine C1; SC; Mice; 2002; 28 days; Controls received mp w/ saline; functionality of mp verified by GC/MS of residual drug; pumps replaced after 14 days; animal info (mature Swiss Webster, male); toxicology.

P8795 Takata,Y., Liu,J., Yin,F., Collins,A.R., Lyon,C.J., Lee,C.H., Atkins,A.R., Downes,M., Barish,G.D., Evans,R.M., Hsueh,W.A., Tangirala,R.K. PPAR delta-mediated antiinflammatory mechanisms inhibit angiotensin II-accelerated atherosclerosis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-11-):4277-4282. >>> Angiotensin II; PBS; SC; Mice; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by blood pressure; animal info (LDLR -/-, 3 mos); pump model not specified.

P8866 Terao,S., Yilmaz,G., Stokes,K.Y., Ishikawa,M., Kawase,T., Granger,D.N. Inflammatory and injury responses to ischemic stroke in obese mice. Stroke 2008; 39(-3-):943-950. >>> Leptin, mouse; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma leptin levels; peptides; post op. care (neomycin trisulfate salt hydrate); animal info (young, 5-7 wks old, ob/ob); obesity.

P9236 Uchiyama,Y., Yoshida,H., Koike,N., Hayakawa,N., Sugita,A., Nishimura,T., Mihara,M. Anti-IL-6 receptor antibody increases blood IL-6 level via the blockade of IL-6 clearance, but not via the induction of IL-6 production. INTERNATIONAL IMMUNOPHARMACOLOGY 2008; 8(-11-):1595-1601. >>> Interleukin-6, recomb. human; SC; Mice; 1002; 9-10 days; Functionality of mp verified by serum hIL-6 levels; immunology; peptides; animal info (male, C57BL/6, IL-6 deficient).

P9181 Van Vliet,E.A., Van Schaik,R., Edelbroek,P.M., da Silva,F.H.L., Wadman,W.J., Gorter,J.A. Development of tolerance to levetiracetam in rats with chronic epilepsy. Epilepsia 2008; 49(-7-):1151-1159. >>> Levetiracetam; Water, distilled; SC; Rat; 2ML1; 2 weeks; Functionality of mp verified; pumps replaced after 1 week; tolerance; animal info (male, Sprague Dawley, adult, 500-700 g.); "flow rate of each pump was verified after removal and corresponded to the range given by the manufacturer indicating that all minipumps functioned properly." pg. 1156.

P8876 Viquez,O.M., Valentine,H.L., Amarnath,K., Milatovic,D., Valentine,W.M. Copper accumulation and lipid oxidation precede inflammation and myelin lesions in N,N-diethyldithiocarbamate peripheral myelinopathy. TOXICOLOGY AND APPLIED PHARMACOLOGY 2008; 229(-1-):77-85. >>> Diethyldithiocarbamate, sodium; IM (abdominal); Rat; 2ML4; 2, 4, 8 weeks; Functionality of mp verified by residual volume, visual inspection for blockage; long-term study; pumps replaced after 4 weeks; animal info (male, Sprague Dawley).

P8852 Wang,H.Y., Ghosh,A., Baigude,H., Yang,C.S., Qiu,L.H., Xia,X.G., Zhou,H.X., Rana,T.M., Xu,Z.S. Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression. Journal of Biological Chemistry 2008; 283(-23-):15845-15852. >>> RNA, small interfering, modified; RNA, small interfering; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice (transgenic); 1007D; 2004; 7, 28 days; 72 hours; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (Fig. 3); no stress (see pg. 15846, 15849); stability verified by 28 days in vivo (see Fig. 2); half-life (p. 15846) "short"; gene therapy; brain tissue distribution; animal info (SOD1G93A Tg); neurodegenerative (ALS); mp + catheter positioning confirmed; Target (SOD1); "when infused at disease onset at the therapeutic dose for 4 weeks, this siRNA slows disease progression without detectable adverse effects." The catheter was implanted between the L5 and L6 vertebra and connected to a primed Alzet osmotic pump with the PE50 tube. The catheter was stitched to the surface muscle, and the Alzet osmotic pumps were placed under the skin on the back of the mouse..

P9186 White,R.E., Yin,F.Q., Jakeman,L.B. TGF-alpha increases astrocyte invasion and promotes axonal growth into the lesion following spinal cord injury in mice. Experimental Neurology 2008; 214(-1-):10-24. >>> Transforming growth factor-alpha; Serum, mouse; PBS; CSF/CNS (intrathecal); Mice; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; post op. care (Baytril); animal info (female, adult, C57BL/6, 10 wks old, 17-20 g.); spinal cord injury; pumps were primed in saline at 37 degree Celsius for 24 hours; pumps were weighed before and after filling; good methods; PE-50 used for catheter.

Q0578 Wu,S., Sasaki,A., Yoshimoto,R., Kawahara,Y., Manabe,T., Kataoka,K., Asashima,M., Yuge,L. Neural stem cells improve learning and memory in rats with Alzheimer's disease. PATHOBIOLOGY 2008; 75(-3-):186-194. >>> Okadaic acid; CSF, artificial; CSF/CNS; Rat; 2004; 56 days; Controls received mp w/ vehicle; animal info (male, Wistar, 270-290 g); functionality of mp verified by total infusion volume; long-term study; pumps replaced after 28 days; neurodegenerative (Alzheimer's Disease); behavioral testing (Morris Water Maze Test, Open-Field Task); chronic Alzheimer's Disease animal model.

P9465 Xiao,W.H., Bennett,G.J. C-fiber spontaneous discharge evoked by chronic inflammation is suppressed by a long-term infusion of lidocaine yielding nanogram per milliliter plasma levels. Pain 2008; 137(-1-):218-228. >>> Lidocaine HCl; SC; Rat; 2001; 7 days; Controls received mp w/ saline; functionality of mp verified by plasma lidocaine levels; dose-response (p. 221); no stress (see pg. 221); animal info (male, Sprague Dawley, 250-350 g.); behavioral testing (heat sensitivity, mechano-allodynia, mechano-hyperalgesia); "all animals exposed to this plasma level gained weight normally and no side-effects were noted." (p. 221).

P9274 Zaher,H., Schwabedissen,H.E.M.Z., Tirona,R.G., Cox,M.L., Obert,L.A., Agrawal,N., Palandra,J., Stock,J.L., Kim,R.B., Ware,J.A. Targeted disruption of murine organic anion-transporting polypeptide 1b2 (oatp1b2/Slco1b2) significantly alters disposition of prototypical drug substrates pravastatin and rifampin. MOLECULAR PHARMACOLOGY 2008; 74(-2-):320-329. >>> Rifampin; pravastatin; Water; DMSO; SC; Mice; 2001D; 24 hours; Functionality of mp verified by plasma concentrations; dose-response (fig. 5); comparison of IV injections vs. mp; animal info (male, DBA1/lacJ, wt, Slco1b2 -/-, 9-14 wks old); 0.5% DMSO used.

P9674 Zerwes,H.G., Li,J., Kovarik,J., Streiff,M., Hofmann,M., Roth,L., Luyten,M., Pally,C., Loewe,R.P., Wieczorek,G., Baenteli,R., Thoma,G., Luckow,B. The chemokine receptor Cxcr3 is not essential for acute cardiac allograft rejection in mice and rats. AMERICAN JOURNAL OF TRANSPLANTATION 2008; 8(-8-):1604-1613. >>> Cyclosporin A; NIBR2130; SC; Mice; 2002; 2ML2; 14 days; Animal info (CXCR3 deficient, C47BL/6, 8-12 wks old); NIBR2130 is a CXCR3 inhibitor; functionality of mp verified by plasma drug levels.

P9265 Zhang,M., Hallows,J.L., Wang,X.Z., Bu,B.T., Wang,W., Vincent,I. Mitogen-activated protein kinase activity may not be necessary for the neuropathology of Niemann-Pick type C mice. Journal of Neurochemistry 2008; 107(-3-):814-822. >>> PD-98059; DMSO; saline; dye, Evan's blue; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by dye infusion; dose-response (fig. 1); enzyme inhibitor (MAPK/ERK 1); brain tissue distribution; animal info (female, BALB/c, Nctr-Npc, 5 wks old); 50% DMSO used; behavioral testing (limb motor activity/coat hanger test).