References on the Use of ALZET® Osmotic Pumps in Gene Therapy

16294 Caviness, K., Bughio, F., Crawford, L. B., Streblow, D. N., Nelson, J. A., Caposio, P., Goodrum, F. Complex Interplay of the UL136 Isoforms Balances Cytomegalovirus Replication and Latency. MBio 2016; 7(-2-):e01986. >>> Colony-stimulating factor, G; AMD3100; IP; Mice (NOD/SCID); 1007D; animal info (NOD-scid humanized (huNSG) mice); gene therapy; immunology; Engraftment of human CD45+ cells; viral persistence; Dose (300mg/ml Colony-stim; 125 ug AMD3100);.

16261 Sztainberg, Y., Chen, H. M., Swann, J. W., Hao, S., Tang, B., Wu, Z., Tang, J., Wan, Y. W., Liu, Z., Rigo, F., Zoghbi, H. Y. Reversal of phenotypes in MECP2 duplication mice using genetic rescue or antisense oligonucleotides. Nature 2015; 528(-7580-):123-6. >>> Oligonucleotide, antisense MECP2; Saline; CSF/CNS; mice; 1004; Controls received mp w/ vehicle; animal info (FVB/N pure background); functionality of mp verified by EEG; ALZET brain infusion kit 3 used; ALZET brain infusion kit 3 used; good methods (pg. 6); gene therapy; Dose (500 ug); Brain coordinates; AP =− 0.2 mm, ML = 1 mm, DV = − 3 mm.

16131 Gujrati, M., Vaidya, A., Lu, Z. R. Multifunctional pH-Sensitive Amino Lipids for siRNA Delivery. Bioconjug Chem 2016; 27(-1-):19-35. >>> RNA, small interfering/EHCO; PEGylated EHCO; Mice (nude); Controls received treated with nonspecific PEGylated EHCO/siGFP nanoparticles (PEGGFP) and non-PEGylated EHCO/HIF-1ǝ; cancer; gene therapy, RNA nanoparticle infusion; peptides;“These results indicate that PEGylation can significantly improve the stability of EHCO/siRNA nanoparticles during storage in solution, possibly by preventing the aggregation of the nanoparticles and providing better protection to the siRNA cargo from degradation” (pg 31);.

16115 Dusabineza, A. C., Najimi, M., van Hul, N., Legry, V., Khuu, D. N., van Grunsven, L. A., Sokal, E., Leclercq, I. A. Hepatic Stellate Cells Improve Engraftment of Human Primary Hepatocytes: A Preclinical Transplantation Study in an Animal Model. Cell Transplant 2015; 24(-12-):2557-71. >>> Uridine, bromodeoxy-; IP; mice; 1004; animal info: SCID mice, females, 20–24 g; GFP+ transgenic mice, males, 35–44 g; gene therapy;.

16010 Fallowfield, J. A., Hayden, A. L., Snowdon, V. K., Aucott, R. L., Stutchfield, B. M., Mole, D. J., Pellicoro, A., Gordon-Walker, T. T., Henke, A., Schrader, J., Trivedi, P. J., Princivalle, M., Forbes, S. J., Collins, J. E., Iredale, J. P. Relaxin modulates human and rat hepatic myofibroblast function and ameliorates portal hypertension in vivo. Hepatology 2014; 59(-4-):1492-504. >>> H2-Relaxin, human recombinant; Sodium acetate; SC; rat; 2ML2; Controls received mp w/ vehicle; minipumps were loaded with either agent or vehicle solution; animal info ( Sprague-dawley (250-300g)); functionality of mp verified by measuring blood levels from the inferior vena cava or portal vein ; dose-response (full dose response curve present; systemic blood levels); post op. care (warmed and received 0.9% NaCl at 10mL/kg/hr); gene therapy; RLN aka Relaxin; dose: (.5 mg/kg/day);.

15710 Jonathan A. Fallowfield,, Annette L. Hayden,, Victoria K. Snowdon,, Rebecca L. Aucott,, Ben M. Stutchfield,, Damian J. Mole,, Antonella Pellicoro,, Timothy T. Gordon-Walker,, Alexander Henke,, Joerg Schrader,, Palak J. Trivedi,, Marc Princivalle,, Stuart J. Forbes,, Jane E. Collins,, and John P. Iredale  Hepatology 2014; 59(-4-):1492-1504. >>> Recombinant human H2-RLN; Sodium acetate; SC; rat; 2ML2; Controls received mp w/ vehicle; sodium acetate pH 5; minipumps were loaded with either agent or vehicle solution; animal info ( Sprague-dawley, 250-300g); functionality of mp verified by plasma level in inferior vena cava or portal vein; dose-response (full dose response curve present; systemic blood levels); post op. care (warmed and received 0.9% NaCl at 10mL/kg/hr); gene therapy; RLN aka Relaxin; dose: (.5 mg/kg/day).

14555 Mathes, A.L., Rice, L., Affandi, A.J., DiMarzio, M., Rifkin, I.R., Stifano, G., Christmann, R.B., Lafyatis, R., Lafyatis, R. CpGB DNA activates dermal macrophages and specifically recruits inflammatory monocytes into the skin. EXPERIMENTAL DERMATOLOGY 2015; 24(--):133-139. >>> CpGB DNA; SC; Mice; Controls received mp w/ PBS; animal info (male, C57BL6 or TNFa -/- or IFNg -/- or CXCR3 -/- or CCR5 -/- or CCL2 -/- or TLR9 -/-); functionality of mp verified by immunoflorescence at pump outlet; gene therapy; immunology; CpGB DNA is a TLR9 ligand;.

13942 Zhang, Q.G., Wang, R.M., Tang, H., Dong, Y., Chan, A., Sareddy, G.R., Vadlamudi, R.K., Brann, D.W., Zhang, Q.G. Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2014; 389(--):84-91. >>> Oligodeoxynucleotide, antisense; Saline; CSF/CNS; Rat; 1007D; Controls received mp w/ control scrambled missense oligonucleotides; animal info (female, Sprague Dawley, 3 months old, ovariectomized); ALZET brain infusion kit 2 used; antisense (oligodeoxynucleorides); ischemia (global cerebral); gene therapy; immunology;.

13927 Sehgal, A., Chen, Q.M., Gibbings, D., Sah, D.W.Y., Bumcrot, D. Tissue-specific gene silencing monitored in circulating RNA. RNA-A PUBLICATION OF THE RNA SOCIETY 2014; 20(-2-):143-149. >>> RNA, small interfering; PBS; CSF/CNS (striatum); Rat; Animal info (male, Sprague Dawley); neurodegenerative (Parkinson's disease); tissue perfusion (striatum); gene therapy; used Plastics One 30g cannula; primed overnight in 37C saline;.

13901 Pileggi, A., Klein, D., Fotino, C., Bravo-Egana, V., Rosero, S., Doni, M., Podetta, M., Ricordi, C., Molano, R.D., Pastori, R.L. MicroRNAs in islet immunobiology and transplantation. Immunologic Research 2013; 57(-1-3-):185-196. >>> Morpholinos; Kidney (renal subcapsular space); Rat; Gene therapy; diabetes; Used silicone catheter 3 french; morpholinos are DNA mimics, miRNA inhibitors.

13889 Ojima, A., Matsui, T., Maeda, S., Takeuchi, M., Inoue, H., Higashimoto, Y., Yamagishi, S. DNA aptamer raised against advanced glycation end products inhibits melanoma growth in nude mice. LABORATORY INVESTIGATION 2014; 94(-4-):422-429. >>> AGE-aptamer; IP; Mice (nude); 1004; Controls received mp w/ control aptamer; animal info (female, nude, athymic, 6 weeks old); comparison of IP injection vs mp; cancer (G361 melanoma); gene therapy; AGE-aptamer aka advanced glycation end products;.

13882 Niimi, N., Kohyama, K., Matsumoto, Y. Minocycline suppresses experimental autoimmune encephalomyelitis by increasing tissue inhibitors of metalloproteinases. NEUROPATHOLOGY 2013; 33(-6-):612-620. >>> RNA, small interfering; IP; Rat; 2002; 2004; Animal info (Lewis, 8-12 weeks old, experimental autoimmune encephalomyelitis); neurodegenerative (multiple sclerosis); gene therapy; immunology;.

13851 Loh, K.P., Ng, G., Yu, C.Y., Fhu, C., Yu, D.J., Vennekens, R., Nilius, B., Soong, T.W., Liao, P. TRPM4 inhibition promotes angiogenesis after ischemic stroke. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2014; 466(-3-):563-576. >>> RNA, small interfering TRPM inhibiting; IV (jugular); Rat; Controls received mp w/ vehicle; animal info (male, Wistar, 300g); ischemia (cerebral); behavioral testing (rotarod apparatus); gene therapy; cardiovascular; TRPM4 aka transient receptor potential melastatin 4;.

13850 Liu, J., Zhang, E., Ma, Z.Y., Wu, W.M., Kosinska, A., Zhang, X.Y., Moeller, I., Seiz, P., Glebe, D., Wang, B.J., Yang, D.L., Lu, M.J., Roggendorf, M. Enhancing Virus-Specific Immunity In Vivo by Combining Therapeutic Vaccination and PD-L1 Blockade in Chronic Hepadnaviral Infection. PLoS Pathogens 2014; 10(-1-):U129-U142. >>> Entecavir; SC; Woodchuck; Animal info (Marmota monax, 1 year old); pumps replaced every 4 weeks; long-term study; gene therapy; immunology;.

13705 Armando, I., Villar, V.M., Jones, J.E., Lee, H., Wang, X.Y., Asico, L.D., Yu, P.Y., Yang, J., Escano, CS Jr, Pascua-Crusan, A.M., Felder, R.A., Jose, P.A., Villar, V.M. Dopamine D3 receptor inhibits the ubiquitin-specific peptidase 48 to promote NHE3 degradation. FASEB Journal 2014; 28(--):1422-1434. >>> GR103691; RNA, small interfering; Transfection reagent (TransIT); SC; kidney (subcapsular space); Mice; 1007D; Controls received mp w/ vehicle or nonsilencing"mock siRNA"; animal info (male, C57BL6J, adult); tissue perfusion (subcapsular space; kidney); gene therapy; antihypertensive; GR103691 is a D3R antagonist; used PE tubing #0007701; pump sutured to abdominal wall; surgical glue applied at puncture site to hold catheter tubing in place and to prevent leakage;.

13613 Nadeau, J.R., Wilson-Gerwing, T.D., Verge, V.M.K. Induction of a reactive state in perineuronal satellite glial cells akin to that produced by nerve injury is linked to the level of p75NTR expression in adult sensory neurons. Glia 2014; 62(-5-):763-777. >>> Oligonucleotide, antisense p75; PBS; rat serum albumin; streptomycin; penicillin; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ control oligonucleotide; animal info (male, Wistar, 250-300g); functionality of mp verified by fluorescent tagged AS; antisense (oligonucleotide p75); post op. care (Temgesic SC 0.1-0.2 mg/kg); gene therapy; used silastic tubing; pumps and tubing primed overnight in PBS; 100U/ml streptomycin; 100U/ml penicillin; 1mg/ul rat serum albumin;.

13593 Crepin, D., Benomar, Y., Riffault, L., Amine, H., Gertler, A., Taouis, M. The over-expression of miR-200a in the hypothalamus of ob/ob mice is linked to leptin and insulin signaling impairment. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2014; 384(-1-2-):1-11. >>> miR-200a phosphorotiate, anti-; Saline; CSF/CNS; Mice; 1002; Controls received mp w/ vehicle; animal info (male, Lep ob/ob C57BL/6, 9 weeks old)); functionality of mp verified by miRNA array; behavioral testing (food intake); gene therapy; diabetes; used dental cement and stainless steel screws;.

13490 Velu, C.S., Chaubey, A., Phelan, J.D., Horman, S.R., Wunderlich, M., Guzman, M.L., Jegga, A.G., Zeleznik-Le, N.J., Chen, J.J., Mulloy, J.C., Cancelas, J.A., Jordan, C.T., Aronow, B.J., Marcucci, G., Bhat, B., Gebelein, B., Grimes, H.L. Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity. Journal of Clinical Investigation 2014; 124(-1-):222-236. >>> Antagomir, miR-21; antagomir, miR-196b; Mice; 2006; Controls received mp w/control antagomirs; functionality of mp verified by analysis of peripheral white blood cells; cancer (leukemia); gene therapy; immunology;"Antagomir-treated mice did not show alteration in their normal behavior or vital organ function, as evidenced by serum chemistry panels and metabolites"pg. 226; long-term study;.

13481 Briones, T.L., Woods, J., Wadowska, M. Chronic neuroinflammation and cognitive impairment following transient global cerebral ischemia: role of fractalkine/CX3CR1 signaling. Journal of Neuroinflammation 2014; 11(-;-):U1-U13. >>> RNA, small interfering CX3CR1; RNA, scrambled; Transfection medium; CSF/CNS; Rat; 1002; Animal info (male, Wistar, 350-375g); ALZET brain infusion kit 2 used; ischemia (cerebral); post op. care (heating pad); behavioral testing (Morris water maze); gene therapy; immunology; Cannula stabilized using dental cement. Pumps primed in sterile saline 37C overnight..

13466 Qiu, J., Zhou, X.Y., Zhou, X.G., Cheng, R., Liu, H.Y., Li, Y. Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy. BIOMED RESEARCH INTERNATIONAL 2013; ;(-;-):U1-U5. >>> Oligonucleotide, miR-210 mimic; inhibitor, miR-210; Saline; CSF/CNS; Rat (neonate); 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, P7); ischemia (hypoxic-ischemic encephalopathy); gene therapy;.

13390 Kaida, Y., Fukami, K., Matsui, T., Higashimoto, Y., Nishino, Y., Obara, N., Nakayama, Y., Ando, R., Toyonaga, M., Ueda, S., Takeuchi, M., Inoue, H., Okuda, S., Yamagishi, S. DNA Aptamer Raised Against AGEs Blocks the Progression of Experimental Diabetic Nephropathy. Diabetes 2013; 62(-9-):3241-3250. >>> Advanced glycation end products aptamer; IP; Mice; 1004; Controls received mp w/ control aptamer; animal info (male, KKay/TA or C57BL6J, 8 weeks old); functionality of mp verified by serum AGE levels; half-life 7 days (p. 3241); gene therapy; diabetes; Advanced glycation end products aptamer aka AGEs-aptamer; Pumps removed after 7 day infusion.

13369 DeVos, S.L., Goncharoff, D.K., Chen, G., Kebodeaux, C.S., Yamada, K., Stewart, F.R., Schuler, D.R., Maloney, S.E., Wozniak, D.F., Rigo, F., Bennett, C.F., Cirrito, J.R., Holtzman, D.M., Miller, T.M. Antisense Reduction of Tau in Adult Mice Protects against Seizures. Journal of Neuroscience 2013; 33(-31-):12887-12897. >>> Tau, ASO-3; Oligonucleotides, antisense scrambled; Saline; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (C57BL6J, Tao -/-); antisense (oligonucleotides); neurodegenerative (Alzheimer's Disease); behavioral testing (elevated plus maze, Morris water maze, seizure score); gene therapy; brain tissue distribution;.

12401 Yun, J., Sonabend, A.M., Ulasov, I.V., Kim, D.H., Rozhkova, E.A., Novosad, V., Dashnaw, S., Brown, T., Canoll, P., Bruce, J.N., Lesniak, M.S. A novel adenoviral vector labeled with superparamagnetic iron oxide nanoparticles for real-time tracking of viral delivery. JOURNAL OF CLINICAL NEUROSCIENCE 2012; 19(-6-):875-880. >>> Rhodamine-dextran; protein, Ad5-green flourescent; Rat; 2ML1; Animal info (male, Harlan Sprague Dawley, adult); MRI; gene therapy.

10858 Chen, Q.M., Butler, D., Querbes, W., Pandey, R.K., Ge, P., Maier, M.A., Zhang, L.G., Rajeev, K.G., Nechev, L., Kotelianski, V., Manoharan, M., Sah, D.W.Y. Lipophilic siRNAs mediate efficient gene silencing in oligodendrocytes with direct CNS delivery. JOURNAL OF CONTROLLED RELEASE 2010; 144(-2-):227-232. >>> RNA, small interfering; cholesterol-conjugate siRNA; PBS; CSF/CNS (corpus callosum); Rat; 2ML1; Controls received mp w/ vehicle; gene therapy; animal info (Male Sprague-Dawley); tissue perfusion (parenchyma); Agents are CNPase siRNA, Cholesterol-CNPas siRNA, Cholesterol-Luciferase siRNA.

9754 Uchibori, R., Okada, T., Ito, T., Urabe, M., Mizukami, H., Kume, A., Ozawa, K. Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy. JOURNAL OF GENE MEDICINE 2009; 11(-5-):373-381. >>> Ganciclovir; IP; Mice (nude); Controls received mp w/ PBS; animal info (6 wks old, male, Balb/c, nu/nu); gene therapy.

9096 Kobayashi, M., Okada, T., Murakami, T., Ozawa, K., Kobayashi, E., Morita, T. Tissue-targeted in vivo gene transfer coupled with histone deacetylase inhibitor depsipeptide (FK228) enhances adenoviral infection in rat renal cancer allograft model systems. UROLOGY 2007; 70(-6-):1230-1236. >>> Ganciclovir; IP; Rat; Gene therapy; animal info (male, ACI, 6-8 wks old).

9083 Wang, H.Y., Ghosh, A., Baigude, H., Yang, C.S., Qiu, L.H., Xia, X.G., Zhou, H.X., Rana, T.M., Xu, Z.S. Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression. Journal of Biological Chemistry 2008; 283(-23-):15845-15852. >>> RNA, small interfering, modified; RNA, small interfering; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice (transgenic); 1007D; 2004; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (Fig. 3); no stress (see pg. 15846, 15849); stability verified by 28 days in vivo (see Fig. 2); half-life (p. 15846)"short"; gene therapy; brain tissue distribution; animal info (SOD1G93A Tg); neurodegenerative (ALS); mp + catheter positioning confirmed; Target (SOD1);"when infused at disease onset at the therapeutic dose for 4 weeks, this siRNA slows disease progression without detectable adverse effects."The catheter was implanted between the L5 and L6 vertebra and connected to a primed Alzet osmotic pump with the PE50 tube. The catheter was stitched to the surface muscle, and the Alzet osmotic pumps were placed under the skin on the back of the mouse..

8743 Neal, Z.C., Sondel, P.M., Bates, M.K., Gillies, S.D., Herweijer, H. Flt3-L gene therapy enhances immunocytokine-mediated antitumor effects and induces long-term memory. CANCER IMMUNOLOGY IMMUNOTHERAPY 2007; 56(-11-):1765-1774. >>> Interleukin-2, recomb. human; SC; Mice; 2001; Controls received mp w/ no treatment; cancer (neuroblastoma); peptides; animal info (female, A/J, ICR, 6-8 weeks old); gene therapy.

8711 Djalilian, H.R., Caicedo, E., Lessan, K., Grami, V., Le, C.T., Spellman, S.R., Pambuccian, S., Hall, W.A., Low, W.C., Ondrey, F.G. Efficacy of an osmotic pump delivered, GM-CSF-based tumor vaccine in the treatment of upper aerodigestive squamous cell carcinoma in rats. CANCER IMMUNOLOGY IMMUNOTHERAPY 2007; 56(-8-):1207-1214. >>> Colony-stimulating factor, GM, murine; interleukin-12; PBS; SC; Rat; Controls received mp w/ vehicle; dose-response (fig 1); no stress (see pg. 1209); cancer (upper aerodigestive tract carcinoma); peptides; animal info (Fisher 344, 125-150 g); good methods;"This latter method (mp) has several advantages. First, the use of minipumps obviates the cumbersome need to transfect tumor cells and completely characterize their cytokine repertoires. Second, it allows for independent and rigorous control over the kinetics of administration of cytokine and antigen dosages. Third, it may generate les controversy than those techniques requiring"gene therapy"IRB approval."(p. 1213)..

8332 Iwasaki, Y., Ueda, M., Yamada, T., Kondo, A., Seno, M., Tanizawa, K., Kuroda, S., Sakamoto, M., Kitajima, M. Gene therapy of liver tumors with human liver-specific nanoparticles. Cancer Gene Therapy 2007; 14(-1-):74-81. >>> Ganciclovir; Water, sterile; SC; Rat (nude); Controls received mp w/ agent and plasmid w/out gene therapy gene; no stress (see p.78); cancer (liver); gene therapy; animal info (male, F344/N, nu/nu, 5 wks old, 180g., NuE hepatic tumors); GCV acts as pro-drug for HSV-tk expression plasmid gene therapy.

8154 Bondanza, A., Valtolina, V., Magnani, Z., Ponzoni, M., Fleischhauer, K., Bonyhadi, M., Traversari, C., Sanvito, F., Toma, S., Radrizzani, M., La Seta-Catamancio, S., Ciceri, F., Bordignon, C., Bonini, C. Suicide gene therapy of graft-versus-host disease induced by central memory human T lymphocytes. Blood 2006; 107(-5-):1828-1836. >>> Ganciclovir; SC; Mice (NOD/SCID); 2001; Controls received mp w/ saline; animal info (female, NOD/SCID, 6-8 weeks old, GvHD); gene therapy.

7705 Burchardt, M., Burchardt, T., Anastasiadis, A.G., Buttyan, R., de la Taille, A., Shabsigh, A., Frank, J., Shabsigh, R. Application of angiogenic factors for therapy of erectile dysfunction: protein and DNA transfer of VEGF 165 into the rat penis. UROLOGY 2005; 66(-3-):665-670. >>> Vascular endothelial growth factor, human 165; Saline, sterile; heparin; IA (renal); Rat; 2004; Controls received mp w/ vehicle; functionality of mp verified by VEGF serum immunoassay; comparison of CDNA plasmid micro-injections vs. mp; gene therapy; peptides; animal info (male, Sprague-Dawley); urology; 2000 Units of heparin used.

7603 Thakker, D.R., Natt, F., Huesken, D., van der Putten, H., Maier, R., Hoyer, D., Cryan, J.F. siRNA-mediated knockdown of the serotonin transporter in the adult mouse brain. Molecular Psychiatry 2005; 10(-8-):782-789. >>> RNA, small interfering; citalopram; RNA, mm; RNAi buffer, isotonic; CSF/CNS (dorsal third); Mice; 1002; Controls received mp w/ vehicle; gene therapy; downregulation of SERT; animal info (male, BALB/C, 19-29 g);"maximally effective RNAi response requires 2 weeks of siRNA infusion."P. 783; behavioral study.

7545 Yin, H., Chao, L., Chao, J. Kallikrein/kinin protects against myocardial apoptosis after ischemia/reperfusion via Akt-glycogen synthase kinase-3 and Akt-Bad.14-3-3 signaling pathways*. Journal of Biological Chemistry 2005; 280(-9-):8022-8030. >>> Virus, adeno-associated; cytomegalovirus promoter; IP; Rat; Cardiovascular; gene therapy; adenoviral vector harboring the human tissue kallikrein cDNA (ad. CMV-TK).

7458 Lakka, S.S., Gondi, C.S., Dinh, D.H., Olivero, W.C., Gujrati, M., Rao, V.H., Sioka, C., Rao, J.S. Specific interference of urokinase-type plasminogen activator receptor and matrix metalloproteinase-9 gene expression induced by double-stranded RNA results in decreased invasion, tumor growth, and angiogenesis in gliomas. Journal of Biological Chemistry 2005; 280(-23-):21882-21892. >>> RNA, small interfering; RNA, small interfering, uPAR; RNA, small interfering, MMP-9; CSF/CNS; Mice (nude); 1002; Controls received mp w/ PBS or empty vector; cancer (glioblastoma); siRNA is within a plasmid vector (pUM vector); antiangiogenesis; gene therapy.

7325 Luebke, A.E., Foster, P.K., Muller, C.D., Peel, A.L. Cochlear function and transgene expression in the guinea pig cochlea, using adenovirus and adeno-associated virus-directed gene transfer. Human Gene Therapy 2001; 12(--):773-781. >>> Adenovirus vector; virus, adeno-associated; Perilymph, artificial; Ear (scala tympani); Guinea-pig; 2001; Controls received mp w/ vehicle, and no treatment to contralateral ear; no stress (see pg. 778); good methods; gene therapy; cyanoacrylate adhesive; tissue perfusion (scala tympani).

7201 Wolfe, D., Niranjan, A., Trichel, A., Wiley, C., Ozuer, A., Kanal, E., Kondziolka, D., Krisky, D., Goss, J., DeLuca, N., Murphey-Corb, M., Glorioso, J.C. Safety and biodistribution studies of an HSV multigene vector following intracranial delivery to non-human primates. Gene Therapy 2004; 11(-23-):1675-1684. >>> Ganciclovir; SC; Monkey (Rhesus); 2ML1; 2ML2; Pumps replaced after 2 weeks; no stress (see pg. 1677); cancer (glioblastoma); toxicology; MRI; multiple pumps per animal (2); gene therapy.

6977 Thakker, D.R., Natt, F., Huesken, D., Maier, R., Mueller, M., van der Putten, H., Hoyer, D., Cryan, J.F. Neurochemical and behavioral consequences of widespread gene knockdown in the adult mouse brain by using nonviral RNA interference. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-49-):17270-17275. >>> RNA, small interfering; GBR 12909; RNAi buffer; CSF/CNS (dorsal third ventricle); Mice; 1007D; 1002; Controls received mp w/ vehicle; antisense (EGFP, DAT, scrambled sequence); Plastics One cannula used;"Results highlight the temporal effects of siRNA, requiring a constant minipump-mediated infusion in the dorsal third ventricle for a stable and bilateral knockdown of gene expression in the brain."(p. 17275); gene therapy.

6943 Gondi, C.S., Lakka, S.S., Dinh, D.H., Olivero, W.C., Gujrati, M., Rao, J.S., Rao, J.S. RNAi-mediated inhibition of cathepsin B and uPAR leads to decreased cell invasion, angiogenesis and tumor growth in gliomas. ONCOGENE 2004; 23(--):8486-8496. >>> RNA, small interfering; EV/SV vector; PBS; CSF/CNS (intratumoral); Mice (nude); Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioma); gene therapy; antiangiogenesis; siRNA (cathepsin B); pump model not stated (0.25 ul/hr);"...local intracranial delivery of pcu using mini-osmotic pumps effectively inhibited human malignant glioma growth.".

6760 Lakka, S.S., Gondi, C.S., Yanamandra, N., Olivero, W.C., Dinh, D.H., Gujrati, M., Rao, J.S. Inhibition of cathepsin B and MMP-9 gene expression in glioblastoma cell line via RNA interference reduces tumor cell invasion, tumor growth and angiogenesis. ONCOGENE 2004; 23(-27-):4681-4689. >>> RNA, small interfering; human cytomegalovirus promoter; Empty vector; CSF/CNS; Mice; 2004; Cancer (glioblastoma); siRNA against mmp-9 and cathepsin; antiangiogenesis; gene therapy.

6340 Mittoux, V., Quary, S, Monville, C, Lisovoski, F, Poyot, T, Conde, F., Escartin, C., Robichon, R  Journal of Neuroscience 2002; 22(-11-):4478-4486. >>> Nitropropionic acid, 3-; Water, deionized; NaOH; SC; Rat; 2ML4; Controls received mp w/ nothing; gene therapy; neurodegenerative (Huntington's disease); behavioral testing; 3NP had a pH of 7.4.

6298 Smoot, L.M., McCormick, J.K., Smoot, J.C., Hoe, N.P., Strickland, I., Cole, R.L., Barbian, K.D., Earhart, C.A., Ohlendorf, D.H., Veasy, L.G., Hill, H.R., Leung, D.Y.M., Schlievert, P.M., Musser, J.M. Characterization of two novel pyrogenic toxin superantigens made by an acute rheumatic fever clone of Streptococcus pyogenes associated with multiple disease outbreaks. Infection and Immunity 2002; 70(-12-):7095-7104. >>> rSpeL; rSpeM; rSpeC; PBS; SC; Rabbit; rSpeL,M and C are bacteriophage genes; gene therapy;"The miniosmotic pump model of STSS was also used to assess the lethality of rSpeL and rSpeM, since these devices release a constant amount of exotoxin into subcutaneous tissue and hence resemble infection with exotoxin-producing GAS.".

6155 Dorn, G., Patel, S., Wotherspoon, G., Hemmings-Mieszczak, M., Barclay, J., Natt, F.J.C., Martin, P., Bevan, S., Fox, A., Ganju, P., Wishart, W., Hall, J. siRNA relieves chronic neuropathic pain. NUCLEIC ACIDS RESEARCH 2004; 32(-5-):1-6. >>> RNA, small interfering; oligonucleotide, antisense; Saline; CSF/CNS (intrathecal); Rat; Controls received mp w/ missense siRNA; no stress (see pg. 4); antisense (P2X3); gene therapy; siRNA (P2X3); pain research.

6148 Roguin, A., Avivi, A., Nitecki, S., Rubinstein, I., Levy, N.S., Abassi, Z.A., Resnick, M.B., Lache, O., Melamed-Frank, M., Joel, A., Hoffman, A., Nevo, E., Levy, A.P. Restoration of blood flow by using continuous perimuscular infiltration of plasmid DNA encoding subterranean mole rat Spalax ehrenbergi VEGF. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES O F AMERICA 2003; 100(-8-):4644-4648. >>> DNA, naked; vascular endothelial growth factor, DNA; Water; IM; Mice; 2001; Controls received mp w/ saline; tissue perfusion (quadriceps muscle); gene therapy; fenestrated catheter; pump implanted IP;"we have shown that when using plamid DNA, continuous administration is superior to multiple simultaneous IM injections."(p. 4647).

5922 Reynolds, S.D., Hong, K.U., Giangreco, A., Mango, G.W., Guron, C., Morimoto, Y., Stripp, B.R. Conditional clara cell ablation reveals a self-renewing progenitor function of pulmonary neuroendocrine cells. Am J Physiol Lung Cell Mol Physiol 2000; 278(-6-):L1256-L1263. >>> Ganciclovir; Saline; Mice (transgenic); Controls received mp w/ vehicle; gene therapy.

5797 Herraiz, M., Beraza, N., Solano, A., Sangro, B., Montoya, J., Qian, C., Prieto, J., Bustos, M. Liver failure caused by herpes simplex virus thymidine kinase plus ganciclovir therapy is associated with mitochondrial dysfunction and mito chondrial DNA depletion. Human Gene Therapy 2003; 14(-5-):463-472. >>> Ganciclovir; Saline; IP; Rat; 2ML2; Controls received mp w/ saline; gene therapy.

5747 Doetsch, F., Petreanu, L., Caille, I., Garcia-Verdugo, J.M., Alvarez-Buylla, A. EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron 2002; 36(-6-):1021-1034. >>> Epidermal growth factor; Ganciclovir; Saline; BSA; PBS; CSF/CNS; Mice; 1007D; 1002; Controls received mp w/ vehicle; Gene therapy; peptides; EGF was diluted in BSA-containing saline and infused for 7 hours, or 6 days in 1007D pumps; Ganciclovir was diluted in PBS and infused for 6 days via 1002 pumps.

5687 Lalwani, A.K., Han, J.J., Castelein, C.M., Carvalho, G.J., Mhatre, A.N. In vitro and in vivo assessment of the ability of adeno-associated virus-brain-derived neurotrophic factor to enhance spiral ganglion cell survival following ototoxic insult. Laryngoscope 2002; 112(-8-):1325-1334. >>> Virus, adeno-associated; Gene, green fluorescent protein; Gene, brain-derived neurotrophic factor; PBS; Ear (cochlea); Guinea-pig; 1007D; Gene therapy; cochlea cannulated with PE10 attached to PE50; virus contained gene for brain-derived neurotrophic factor and/or green fluorescent protein; tissue perfusion (cochlea).

6380 Melton, L.M., Keith, A.B., Davis, S., Oakley, A.E., Edwardson, J.A., Morris, C.M. Chronic glial activation, neurodegeneration, and APP immunoreactive deposits following acute administration of double-stranded RNA. Glia 2003; 44(-1-):1-12. >>> RNA, double stranded; statolon dsRNA; polyinosinic acid ds RNA; polycytidilic acid dsRNA; Tris/borate/EDTA buffer; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle; gene therapy; post op. care (buprenorphine); statolon is a naturally occuring dsRNA from a mycophage; neurodegenerative (Parkinson's disease, Alzheimer's); 2mM EDTA;.

4931 Luebke, A.E., Steiger, J.D., Hodges, B.L., Amalfitano, A. A modified adenovirus can transfect cochlear hair cells in vivo without compromising cochlear function.. Gene Therapy 2001; 8(--):789-794. >>> Adenovirus; Gene, beta-galactosidase;; Perilymph, artificial;; Ear (cochlea);; guinea-pig;; 2001;; Controls received mp w/ vehicle; tissue perfusion (scala tympani); functionality of mp verified by transgene expression of b-gal; gene therapy;.

4892 Efron, D.T., Most, D., Shi, H.P., Tantry, U.S., Barbul, A. A novel method of studying wound healing.. Journal of Surgical Research 2001; 98(--):16-20. >>> Methylisothiourea, S-; adenovirus vector; gene, mouse iNOS cDNA sequence; Saline; Dye, methlene blue; Dye, India black ink; PBS; SC (wound healing site);; rat;; 2001; 2ML1;; Controls received mp w/ saline; functionality of mp verified by dye infusion; gene therapy; enzyme inhibitor; methylisothiourea,S- is an inducible nitric oxide synthase inhibitor (iNOS inhibitor); wound healing; SC-implanted pumps infused 2 hydroxyproline sponges via catheter; initial studies used 2ML1 pumps to infuse dyes in order to assess the feasibility of direct infusion with pumps; iNOS inhibitor infusion was with 2001 pumps; pumps were designed to infuse directly into SC implanted polyvinyl sponges at the wound site; Adenovirus vector was dissolved in PBS; iNOS inhibitor was delivered in saline; diagram of pump-catheter assembly and location (p. 18);"Dye infusion demonstrated both grossly and microscopically excellent delivery of the infusate to wound sponges"(p. 18);.

4685 Bush, T.G, Puvanachandra, N, Horner, C.H, Polito, A, Ostenfeld, T, Svendsen, C.N, Mucke, L, Johnson, M.H, Sofroniew, M.V  Neuron 1999; 23(--):297-308. >>> Ganciclovir; memantine; Saline, sterile physiological; SC; Mice (transgenic); gene therapy; ganciclovir infused for 7 days; memantin infused for 14 days.

4684 Bush, T.G, Savidge, T.C, Freeman, T.C, Cox, H.J, Campbell, E.A, Mucke, L, Johnson, M.H, Sofroniew, M.V  Cell 1998; 93(--):189-201. >>> Ganciclovir;; Saline, sterile physiological; SC;; mice (transgenic); 2004; 2001; 2002; 1007D; controls received no treatment; gene therapy.

4578 Han, J.J, Mhatre, A.N., Wareing, M, Pettis, R, Gao, W.-Q., Zufferey, R.N, Trono, D, Lalwani, A.K. Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector.. Human Gene Therapy 1999; 10(--):1867-1873. >>> Lentivirus; gene, green fluorescent protein; Saline; PBS;; ear (cochlea);; guinea-pig;; 1007D;; controls received mp w/vehicle; tissue perfusion (scala tympani); gene therapy;.

4574 Cohen, J.L, Boyer, O, Salomon, B, Onclercq, R, Charlotte, F, Bruel, S, Boisserie, G, Klatzmann, D  Blood 1997; 89(-12-):4636-4645. >>> Ganciclovir;; PBS;; SC;; mice;; 2001;; controls received mp w/vehicle; functionality of mp verified by plasma levels; comparison of IP injections vs. mp; immunology; Gene therapy.

4541 Wareing, M, Mhatre, A.N, Pettis, R, Han, J.J, Haut, T, Pfister, M.H.F, Hong, K, Zheng, W.W, Lalwani, A.K  Hearing Research 1999; 128(--):61-69. >>> Liposomes, cationic; Gene, beta-galactosidase; Dextrose solution;; ear;; guinea-pig;; 1007D;; tissue perfusion (cochlea); comparison of micro injections vs. mp; stress/adverse reaction: significant fibrosis and acute immune response localized at the site of cochleostomy; gene therapy; prophylactic antibiotics provided; PE50 tubing was connected to PE10;.

15536 Lalwani, A.K., Walsh, B.J., Reilly, P.G., Carvalho, G.J., Zolotukhin, S., Muzyczka, N., Mhatre, A.N. Long-term in vivo cochlear transgene expression mediated by recombinant adeno-associated virus. Gene Therapy 1998; 5(--):277-281. >>> Virus, adeno-associated; Gene, lacZ; Gene, green fluorescent protein; Saline; ear (cochlea); guinea-pig; controls received mp w/vehicle; tissue perfusion (cochlea); functionality of mp verified by gene expression; gene therapy.

4125 Lalwani, A.K., Walsh, B.J., Reilly, P.G., Carvalho, G.J., Zolotukhin, S., Muzyczka, N., Mhatre, A.N. Long-term in vivo cochlear transgene expression mediated by recombinant adeno-associated virus. Gene Therapy 1998; 5(--):277-281. >>> Virus, adeno-associated; Gene, lacZ; Gene, green fluorescent protein; Saline; ear (cochlea); guinea-pig; controls received mp w/vehicle; tissue perfusion (cochlea); functionality of mp verified by gene expression; gene therapy.

15535 Lalwani, A.K., Walsh, B.J., Carvalho, G.J., Muzyczka, N., Mhatre, A.N. Expression of adeno-associated virus integrated transgene within the mammalian vestibular organs. Am. J. Otology 1998; 19(--):390-395. >>> Virus, adeno-associated; Gene, green fluorescent protein; Gene, beta-galactosidase; PBS; ear (cochlea); guinea-pig; 1007D; controls received mp w/vehicle, mp w/reporter gene, or no pump; tissue perfusion (scala tympani); gene therapy.

4124 Lalwani, A.K., Walsh, B.J., Carvalho, G.J., Muzyczka, N., Mhatre, A.N. Expression of adeno-associated virus integrated transgene within the mammalian vestibular organs. Am. J. Otology 1998; 19(--):390-395. >>> Virus, adeno-associated; Gene, green fluorescent protein; Gene, beta-galactosidase; PBS; ear (cochlea); guinea-pig; 1007D; controls received mp w/vehicle, mp w/reporter gene, or no pump; tissue perfusion (scala tympani); gene therapy.

15428 Benedetti, S., Dimeco, F., Pollo, B., Cirenei, B., Colombo, B.M., Bruzzone, M.G., Cattaneo, E., Vescovi, A., Didonato, S., Colombo, M.P., Finocchiaro, G. Limited efficacy of the hsv-tk/gcv system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Human Gene Therapy 1997; 8(--):1345-1353. >>> Ganciclovir; Water; Rat; mice (nude); 2002; 2ML2; comparison of IP injections vs. mp; cancer; gene therapy.

4017 Benedetti, S., Dimeco, F., Pollo, B., Cirenei, B., Colombo, B.M., Bruzzone, M.G., Cattaneo, E., Vescovi, A., Didonato, S., Colombo, M.P., Finocchiaro, G. Limited efficacy of the hsv-tk/gcv system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Human Gene Therapy 1997; 8(--):1345-1353. >>> Ganciclovir; Water; Rat; mice (nude); 2002; 2ML2; comparison of IP injections vs. mp; cancer; gene therapy.

3957 Zhu, J., Zhang, L., Hanisch, U.K., Felgner, P.L., Reszka, R. A continuous intracerebral gene delivery system for in vivo liposome-mediated gene therapy. Gene Therapy 1996; 3(--):472-476. >>> Liposomes; Gene, herpes simplex virus thymidine kinase; Gene, lacZ; CSF/CNS (caudate nucleus); Rat; 1003D; controls received mp w/LacZ gene; tissue perfusion (tumor); functionality of mp verified by gene expression; comparison of intracerebral injections vs. mp; no stress (see pg.473); stability verified by gene expression; ALZET brain infusion kit used; cancer; gene therapy;"DNA-liposome complexes were stable within minipumps at body temperature (37C) for 1-3 days."(pg.474);"continuous administration of DNA-liposome complexes did not result in significant in vivo toxicity."(pg.474).

3956 Lalwani, A.K., Han, J.J., Walsh, B.J., Zolotukhin, S., Muzyczka, N., Mhatre, A.N. Green fluorescent protein as a reporter for gene transfer studies in the cochlea. Hear. Res 1997; 114(--):139-147. >>> Virus, adeno-associated; Gene, green fluorescent protein; PBS; ear (cochlea); guinea-pig; 1007D; controls received mp w/saline or mp w/AAV-Bgal; tissue perfusion (scala tympani); good methods (p.141); gene therapy.

3681 Franken, M., Estabrooks, A., Cavacini, L., Sherburne, B., Wang, F., Scadden, D.T. Epstein-barr virus-driven gene therapy for EBV-related lymphomas. Nature Medicine 1996; 2(-12-):1379-1382. >>> Ganciclovir; mice (SCID); controls received p w/ saline; comparison of ip injections vs. mp; cancer; gene therapy.

 

Since 1977, scientist around the world have used ALZET pumps to conduct their research, publishing their results in high-impact journals. The ALZET bibliography now contains over 16,000 publications! We can perform a custom search for references relevant to your research.

Request your ALZET bibliography search!

 

Order the ALZET SurgicalImplantation Video

What researchers are saying...

“...In comparison to bolus IP injection, continuous administration of angiogenesis inhibitors by an osmotic pump implanted IP resulted in a decrease of the required dosage and improved the effectiveness of therapy.” (p.451) Cheng et al., Neoplasia 2003;5:445-456