References on the Intra-Arterial Administration of Agents Using ALZET^® Osmotic Pumps

Q0190 Troidl,C., Nef,H., Voss,S., Schilp,A., Kostin,S., Troidl,K., Szardien,S., Rolf,A., Schmitz-Rixen,T., Schaper,W., Hamm,C.W., Elsaesser,A., Moellmann,H. Calcium-dependent signalling is essential during collateral growth in the pig hind limb-ischemia model. Journal of Molecular and Cellular Cardiology 2010; 49(-1-):142-151. >>> Phorbol didecanoate, 4-alpha-; IA (femoral); Pig; 2ML1; 7 days; Controls received mp w/ saline or sham operation; cardiovascular; animal info (male, juvenile, crossbred, 38kg, femoral artery occlusion); post op. care (cefquinome, fentanyl); ischemia (hind limb); excellent color photograph of multiple mp placement (Fig. 1); multiple pumps per animal (2).

P9738 Croons,V., Martinet,W., Herman,A.G., Timmermans,J.P., De Meyer,G.R.Y. The Protein Synthesis Inhibitor Anisomycin Induces Macrophage Apoptosis in Rabbit Atherosclerotic Plaques through p38 Mitogen-Activated Protein Kinase. Journal of Pharmacology and Experimental Therapeutics 2009; 329(-3-):856-864. >>> Anisomycin; IA (carotid); Rabbit; 2ML1; 3 days; Controls received mp w/saline; animal info (male, New Zealand, White, 3.3-3.9 kg).

P9814 Santulli,G., Ciccarelli,M., Palumbo,G., Campanile,A., Galasso,G., Ziaco,B., Altobelli,G.G., Cimini,V., Piscione,F., D'Andrea,L.D., Pedone,C., Trimarco,B., Iaccarino,G. In vivo properties of the proangiogenic peptide QK. Journal of Translational Medicine 2009; 7(-;-):U1-U10. >>> Vascular endothelial growth factor-15; vascular endothelial growth factor-165; QK; IA (femoral); Rat; 2002; 14 days; Peptides; animal info (12 wks old, WKY, normosensitive); QK is a de novo engineered VEGF mimicking peptide.

Q0378 Troidl,C., Troidl,K., Schierling,W., Cai,W.J., Nef,H., Moellmann,H., Kostin,S., Schimanski,S., Hammer,L., Elsaesser,A., Schmitz-Rixen,T., Schaper,W. Trpv4 induces collateral vessel growth during regeneration of the arterial circulation. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2009; 13(-8B-):2613-2621. >>> PDD, 4 alpha; ruthenium red; Ethanol; NaCl; IA (femoral); Rabbit; rat; 7 days; Controls received mp w/vehicle or sham operated; animal info (New Zealand White, 3.0 kg, Sprague Dawley, 275 g); post op. care (buprenorphine); 4-alpha PDD also known as Phorbol-12,13-didecanoate; Fig 1, schematic of femoral artery ligature with ALZET pump.

P9229 Jiang,M., Bujo,H., Ohwaki,K., Unoki,H., Yarnazaki,H., Kanaki,T., Shibasaki,M., Azuma,K., Harigaya,K., Schneider,W.J., Saito,Y. Ang II-stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice. Journal of Clinical Investigation 2008; 118(-8-):2733-2746. >>> Angiotensin II; candesartan; Saline; IA (femoral); Mice; 4 weeks; Controls received mp w/ vehicle; cardiovascular; peptides; animal info (male, C57BL/6, 20 wks old, Lr11 -/-, wt).

P8461 Chan,E.C., Datla,S.R., Dilley,R., Hickey,H., Drummond,G.R., Dusting,G.J. Adventitial application of the NADPH oxidase inhibitor apocynin in vivo reduces neointima formation and endothelial dysfunction in rabbits. Cardiovascular Research 2007; 75(-4-):710-718. >>> Apocynin; DMSO; IA (carotid); Rabbit; 2001; ; Controls received mp w/vehicle, or no treatment; enzyme inhibitor (NADPH oxidase); cardiovascular; animal info (male, New Zealand White, 3-4kg); 0.1% DMSO; mp attached to catheter to hollow, nonoclusive silastic collar around common cartoid artery.

P8353 Croons,V., Martinet,W., Herman,A.G., Timmermans,J.P., De Meyer,G.R.Y. Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide. Journal of Pharmacology and Experimental Therapeutics 2007; 320(-3-):986-993. >>> Cycloheximide; IA (carotid); Rabbit; 2ML1; 3 days; Controls received mp w/ saline; cardiovascular; animal info (male, New Zealand white, 3.1-3.8 kg); mp connected to a collar around artherosclerotic carotid arteries.

P8299 Cruze,C.A., Su,F., Limberg,B.J., Deutsch,A.J., Stoffolano,P.J., Dai,H.J., Buchanan,D.D., Yang,H.T., Terjung,R.L., Spruell,R.D., Mittelstadt,S.W., Rosenbaum,J.S. The Y2 receptor mediates increases in collateral-dependent blood flow in a model of peripheral arterial insufficiency. Peptides 2007; 28(-2-):269-280. >>> Peptide YY, recomb. human; peptide YY (3-36), recomb. human; vascular endothelial growth factor 165; PBS; glycerol; sodium citrate; Tween 20; IA (iliac); Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (femoral artery occlusion); dose-response (fig. 4); half-lfie (p. 276)<30 minutes; cardiovascular; peptides; ischemia (hindlimb); animal info (male, Sprague-Dawley, 325-350 grams).

P8728 Gradl,G., Finke,B., Schattner,S., Gierer,P., Mittlmeier,T., Vollmar,B. Continuous intra-arterial application of substance P induces signs and symptoms of experimental complex regional pain syndrome (CRPS) such as edema, inflammation and mechanical pain but no thermal pain. Neuroscience 2007; 148(-3-):757-765. >>> Substance P; Saline; heparin; IA (femoral); Rat; 2001D; 24 hours; Controls received mp w/ vehicle; good methods (p. 758); animal info (male, Sprague-Dawley, 225-250g); pain.

P8836 Nichols,T.C., Busby,WH Jr, Merricks,E., Sipos,J., Rowland,M., Sitko,K., Clemmons,D.R. Protease-resistant insulin-like growth factor (IGF)-Binding protein-4 inhibits IGF-I actions and neointimal expansion in a porcine model of neointimal hyperplasia. Endocrinology 2007; 148(-10-):5002-5010. >>> Insulin-like growth factor-binding protein-4; Insulin-like growth factor-binding protein-4, mutated; Insulin-like growth factor-1, recomb. human; PBS; IA (carotid); IA (femoral); Pig; 21 days; Controls received mp w/ vehicle; cardiovascular; peptides; animal info (male, female, spotted Poland/China, 12 months old); protease resistant mutant form of IGFBP-4.

P8306 Tiranathanagul,K., Dhawan,V., Lytle,I.F., Zhang,W., Borschel,G.H., Buffington,D.A., Tziampazis,E., Brown,D.L., Humes,H.D. Tissue engineering of an implantable bioartificial hemofilter. ASAIO Journal 2007; 53(-2-):176-186. >>> Platelet-derived growth factor-BB; vascular endothelial growth factor 164; Saline, normal; albumin, rat serum; acetic acid; IA (femoral); IV (femoral); Rat; 2004; 28 days; Artificial kidney; controls received mp w/ vehicle; peptides; animal info (male, Fisher 344); mp attached by cannula to bioartificial hemofilter device, which surrounded and delivered agent to the femoral vessels; nephrology.

P7731 Carr,A.N., Howard,B.W., Yang,H.T., Eby-Wilkens,E., Loos,P., Varbanov,A., Qu,A., DeMuth,J.P., Davis,M.G., Proia,A., Terjung,R.L., Peters,K.G. Efficacy of systemic administration of SDF-1 in a model of vascular insufficiency: Support for an endothelium-dependent mechanism. Cardiovascular Research 2006; 69(-4-):925-935. >>> Vascular endothelial growth factor; stromal cell-derived factor-1a; PBS; glycerol; sodium acetate; sodium azide; IA (iliac); Rat; 2 weeks; Controls received mp w/ vehicle; dose-response (table 1); cardiovascular; peptides; animal info (adult); bilateral femoral artery ligation; occlusive cardiovascular disease.

P7495 Burchardt,M., Burchardt,T., Anastasiadis,A.G., Buttyan,R., de la Taille,A., Shabsigh,A., Frank,J., Shabsigh,R. Application of angiogenic factors for therapy of erectile dysfunction: protein and DNA transfer of VEGF 165 into the rat penis. UROLOGY 2005; 66(-3-):665-670. >>> Vascular endothelial growth factor, human 165; Saline, sterile; heparin; IA (renal); Rat; 2004; 28 days; Controls received mp w/ vehicle; functionality of mp verified by VEGF serum immunoassay; comparison of CDNA plasmid micro-injections vs. mp; gene therapy; peptides; animal info (male, Sprague-Dawley); urology; 2000 Units of heparin used.

P7576 Zhao,L.R., Spellman,S., Kim,J., Duan,W.M., McCarthy,J.B., Low,W.C. Synthetic fibronectin peptide exerts neuroprotective effects on transient focal brain ischemia in rats. Brain Research 2005; 1054(-1-):1-8. >>> Fibronectin-derived peptide, synthetic; PBS; IA (carotid); Rat; 24 hours; Controls received mp w/ vehicle or control peptide PRARI; peptides; ischemia (cerebral); animal info (male, Sprague-Dawley, 300-350 g); peptide: PRARIY; MCAO.

P6370 Manoonkitiwongsa,P.S., Schultz,R.L., McCreery,D.B., Whitter,E.F., Lyden,P.D. Neuroprotection of ischemic brain by vascular endothelial growth factor is critically dependent on proper dosage and may be compromised by angiogenesis. Journal of Cerebral Blood Flow and Metabolism 2004; 24(-6-):693-702. >>> Vascular endothelial growth factor; Saline; IA (carotid); Rat; 2001; 7 days; Controls received mp w/ vehicle; dose response (p. 697); stroke; ischemia (cerebral); biocompatible glue was used to secure catheter in artery.

P6436 Porro,C.A., Cavazzuti,M., Giuliani,D., Vellani,V., Lui,F., Baraldi,P. Effects of ketamine anesthesia on central nociceptive processing in the rat: A 2-deoxyglucose study. Neuroscience 2004; 125(-2-):485-494. >>> Heparin; Saline; IA (carotid); IV (jugular); Rat; 2001; 7 days; Controls received mp w/ vehicle; post op. care (wounds treated with local anesthetics, lidocaine cream); PE-50 tubing was filled with rat serum and heparin and plugged with nylon fishing line until the pumps were attached; pumps were used only as a means of keeping vessel catheters patent..

P6784 Schirmer,S.H., Buschmann,I.R., Jost,M.M., Hoefer,I.E., Grundmann,S., Andert,J.P., Ulusans,S., Bode,C., Piek,J.J., van Royen,N. Differential effects of MCP-1 and leptin on collateral flow and arteriogenesis. Cardiovascular Research 2004; 64(-2-):356-364. >>> Monocyte chemoattractant protein-1, recomb. human; leptin, recomb. human; IA (femoral); Rabbit; 2ML1; 7 days; Controls received mp w/ PBS; no stress (see pg. 359); cardiovascular; peptides; right femoral artery occlusion.

P6394 Sudoh,M., Kishimoto,Y., Marumoto,B., Inoue,M., Sano,A., Miura,N., Horie,Y., Hasegawa,J., Ryoke,K. A new animal model of continuous catheterization for investigating mechanisms of arteritis associated with chemotherapy. LIFE SCIENCES 2004; 74(-24-):3025-3032. >>> Fluorouracil, 5-FU; peplomycin; Saline, heparinized; IA (abdominal aorta); Rat; 2ML1; 7 days; Controls received mp w/ vehicle; cancer; chemotherapeutic agents; PE-10 and 60 used; tubing attached using a moment binding agent (alon-alfa); catheter schematic p. 3027; pump placed IP.

P5722 Arras,M., Ito,W.D., Scholz,D., Winkler,B., Schaper,J., Schaper,W. Monocyte activation in angiogenesis and collateral growth in the rabbit hindlimb. J Clin.Invest 2003; 101(-1-):40-50. >>> Uridine, bromodeoxy-; IA (carotid); Rabbit; 2ML2; 3,7 days; Study proliferation of collateral arteries and capillaries.

P5729 Boengler,K., Pipp,F., Fernandez,B., Richter,A., Schaper,W., Deindl,E. The ankyrin repeat containing SOCS box protein 5: a novel protein associated with arteriogenesis. Biochemical and Biophysical Research Communications 2003; 302(-1-):17-22. >>> Monocyte chemoattractant protein-1; Transforming Growth Factor-B; Doxorubicin; IA (femoral); Rabbit; 2ML1; 24 hours; Cardiovascular; peptides; MCP-1 is monocyte chemoattractant protein 1.

P6033 Boengler,K., Pipp,F., Fernandez,B., Ziegelhoeffer,T., Schaper,W., Deindl,E. Arteriogenesis is associated with an induction of the cardiac ankyrin repeat protein (carp). Cardiovascular Research 2003; 59(-3-):573-581. >>> Monocyte chemoattractant protein-1; doxorubicin; transforming growth factor-B1; IA (femoral); Rabbit; 2ML1; 24 hours; Cardiovascular.

P5730 Buckwalter,J.B., Curtis,V.C., Valic,Z., Ruble,S.B., Clifford,P.S. Endogenous vascular remodeling in ischemic skeletal muscle: a role for nitric oxide. Journal of Applied Physiology 2003; 94(-3-):935-940. >>> L-NAME; D-NAME; Phenylephrine; Saline; heparin; IA (femoral); Rabbit; 2ML2; 6 weeks; Controls received mp w/ phenylephrine or D-NAME; enzyme inhibitor (Nitric oxide synthase); cardiovascular; peptides; L-NAME&D-NAME (no synthase inhibitors) were dissolved in heparinized saline (100 U/ml).

P6123 Cao,R.H., Brakenhielm,E., Pawliuk,R., Wariaro,D., Post,M.J., Wahlberg,E., Leboulch,P., Cao,Y.H. Angiogenic synergism, vascular stability and improvement of hind-limb ischemia by a combination of PDGF-BB and FGF-2. Nature Medicine 2003; 9(-5-):604-613. >>> Fibroblast growth factor; platelet-derived growth factor; IA (femoral); Rabbit; 7 days; ischemia (hind-limb).

P5787 Deindl,E., Hoefer,I.E., Fernandez,B., Barancik,M., Heil,M., Strniskova,M., Schaper,W. Involvement of the fibroblast growth factor system in adaptive and chemokine-induced arteriogenesis. Circulation Research 2003; 92(-5-):561-568. >>> Polyanetholesulfonic Acid; Monocyte chemoattractant protein-1; IA (femoral); Rabbit; 1 week; Controls received mp w/ PBS; cardiovascular; MCP-1.

P5682 Heeschen,C., Weis,M., Cooke,J.P. Nicotine promotes arteriogenesis. Journal of the American College of Cardiology 2003; 41(-3-):489-496. >>> Nicotine; fibroblast growth factor, basic; PBS; IA (iliac); Rabbit; 2ML2; 18 days; Controls received mp w/ vehicle..

P5645 Pipp,F., Heil,M., Issbrucker,K., Ziegelhoeffer,T., Martin,S., van den Heuvel,J., Weich,H., Fernandez,B., Golomb,G., Carmeliet,P., Schaper,W., Clauss,M. VEGFR-1-selective VEGF homologue PlGF is arteriogenic - Evidence for a monocyte-mediated mechanism. Circulation Research 2003; 92(-4-):378-385. >>> Vascular endothelial growth factor; Vascular endothelial growth factor-E; Monocyte chemoattractant protein-1; Placental growth factor; Phosphate buffer; albumin; IA (femoral); Mice; rabbit; 1 week; Controls received mp w/ vehicle; dose-response (p.381); peptides; placenta growth factor (PlGF) is a VEGF homologue; VEGF-E is a chimera containing the heparin-binding domain of VEGF; MCP-1.

P6621 Prior,B.M., Lloyd,P.G., Ren,J., Li,Z., Yang,H.T., Laughlin,M.H., Terjung,R.L. Arteriogenesis: Role of nitric oxide. ENDOTHELIUM-JOURNAL OF ENDOTHELIAL CELL RESEARCH 2003; 10(-4-5-):207-216. >>> Fibroblast growth factor; heparinase I; IA (iliac); 2 weeks; Dose-response (p. 213); the heparinase I infusion group targeted the contralateral iliac vessel; pump or animal model not mentioned.

P6009 Srivastava,S., Terjung,R.L., Yang,H.T. Basic fibroblast growth factor increases collateral blood flow in spontaneously hypertensive rats. American Journal of Physiology-Heart and Circulatory Physiology 2003; 285(-3-):H1190-H1197. >>> Fibroblast growth factor, basic; Sodium citrate; glycerol; PBS; IA (iliac); Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume p. H1192; antihypertensive; post op. care (topical antibiotic powder); "The pump was housed in a tunnel under the skin in the left groin area; this placement did not hamper hindlimb movement while rats were walking on the treadmill." p. H1191; behavioral study; "There were no signs of ischemia or necrosis and no presence of infection or complications from the osmotic pumps..." p. H1192.

P5864 van Royen,N., Hoefer,I., Buschmann,I., Kostin,S., Voskuil,M., Bode,C., Schaper,W., Piek,J.J. Effects of local MCP-1 protein therapy on the development of the collateral circulation and atherosclerosis in Watanabe hyperlipidemic rabbits. Cardiovascular Research 2003; 57(-1-):178-185. >>> Monocyte chemoattractant protein-1; PBS; IA (femoral); Rabbit; 2ML1; 1 week; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (p. 181, 183); cardiovascular; peptides; MCP-1.

P7695 van,Royen N., Hoefer,I., Bottinger,M., Hua,J., Grundmann,S., Voskuil,M., Bode,C., Schaper,W., Buschmann,I., Piek,J.J. Local monocyte chemoattractant protein-1 therapy increases collateral artery formation in apolipoprotein E-deficient mice but induces systemic monocytic CD11b expression, neointimal formation, and plaque progression. Circ.Res. 2003/2/7; 92(-2-):218-225. >>> Monocyte chemotactic protein-1; IA (femoral); Mice; 1007D; 7 days; Controls received mp w/ PBS; cardiovascular.

P6616 Ziegelhoeffer,T., Scholz,D., Friedrich,C., Helisch,A., Wagner,S., Fernandez,B., Schaper,W. Inhibition of collateral artery growth by mibefradil: Possible role of volume-regulated chloride channels. ENDOTHELIUM-JOURNAL OF ENDOTHELIAL CELL RESEARCH 2003; 10(-4-5-):237-246. >>> Mibefradil; PBS; IA (femoral); Mice; 1007D; 7 days; Controls received mp w/ vehicle; antihypertensive; mibefradil is a tetralol calcium channel blocking agent; PE-50 heat stretched to an O.D of 0.22 mm.

P5051 Ahn,Y.M., Gajdusek,C., London,S., Moon,C.T., Oh,C.W., Mayberg,M.R. Sustained arterial narrowing after prolonged exposure to perivascular endothelin. Neurosurgery 2002; 50(--):843-848. >>> Endothelin-1; Saline; Perivascular (femoral artery adventitia); rat; 3 or 5 days; controls received mp w/ vehicle; tissue perfusion (adventitia); cardiovascular; peptides; ET-1 is a vasoconstrictor peptide; Arteries perfused via a custom silastic artery cuff attached to catheter tube; pump placed sc.

P5087 Dufourcq,P., Couffinhal,T., Alzieu,P., Daret,D., Moreau,C., Duplaa,C., Bonnet,J. Vitronectin is up-regulated after vascular injury and vitronectin blockade prevents neointima formation. Cardiovascular Research 2002; 53(--):952-962. >>> Antibody, monoclonal anti-vitronectin; Antibody, monoclonal anti-B3 integrin; Antibody, monoclonal anti-avB5 integrin; PBS; Immunoglobulin G, non-immune; perivascular (carotid artery adventitia); rat; 7 days; Controls received control immunoglobulin; tissue perfusion (corotid adventitia); cardiovascular; peptides.

P5542 Fernandez-Botran,R., Gorantla,V., Sun,X.C., Ren,X.P., Perez-Abadia,G., Crespo,F.A., Oliver,R., Orhun,H.I., Quan,E.E., Maldonado,C., Ray,M., Barker,J.H. Targeting of glycosaminoglycan-cytokine interactions as a novel therapeutic approach in allotransplantation. Transplantation 2002; 74(-5-):623-629. >>> MC-2; Saline; IA (profunda femoris artery); Rat; 2ML2; ; Controls received mp w/ vehicle; tissue perfusion (skin flap allograft); immunology; peptides; MC-2 is derived from mouse interferon gamma; transplant.

R0188 Heilmann,C., Beyersdorf,F., Lutter,G. Collateral growth: cells arrive at the construction site. CARDIOVASCULAR SURGERY 2002; 10(-6-):570-578. >>> Monocyte chemotactic protein-1; IA; Rabbit; ; Cardiovascular; p. 574.

P5340 Scholz,D., Ziegelhoeffer,T., Helisch,A., Wagner,S., Friedrich,C., Podzuweit,T., Schaper,W. Contribution of arteriogenesis and angiogenesis to postocclusive Hindlimb perfusion in mice. Journal of Molecular and Cellular Cardiology 2002; 34(-7-):775-787. >>> Fibroblast growth factor 2; PBS; IA (femoral); Mice; 1007D; ; Controls received mp w/ vehicle; peptides.

P6224 Seye,C.I., Kong,Q.M., Erb,L., Garrad,R.C., Krugh,B., Wang,M.F., Turner,J.T., Sturek,M., Gonzalez,F.A., Weisman,G.A. Functional P2Y2 nucleotide receptors mediate uridine 5 '-triphosphate-induced intimal hyperplasia in collared rabbit carotid arteries. Circulation 2002; 106(-21-):2720-2726. >>> Uridine 5-triphosphate; IA (carotid); Rabbit; 2ML2; 3,14 days; Controls received mp w/ PBS; cardiovascular; post op. care (buprenorphine).

P4988 Madsen,K.M., Neerhof,M.G., Wessale,J.L., Thaete,L.G. Influence of ETB receptor antagonism on pregnancy outcome in rats. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION 2001; 8(--):239-244. >>> A-192621; Ethanol; Propyene glycol; Sodium hydroxide; IA (carotid); rat (pregnant); 7 days; controls received mp w/ vehicle; functionality of mp verified by plasma A-192621 levels; dose-response (fig 3. p. 242); peptides; A-192621 is an Endothelin-B receptor antagonist which blocks vasoconstriction; vehicle was 20% ethanol, 40% propylene glycol, 0.4 M sodium hydroxide..

P4782 Yang,H.T., Yan,Z., Abraham,J.A., Terjung,R.L. VEGF121- and bFGF-induced increase in collateral blood flow requires normal nitric oxide production.. American Journal of Physiology-Heart and Circulatory Physiology 2001; 280(--):H1097-H1104. >>> Vascular endothelial growth factor 121; Fibroblast growth factor, basic;; PBS; Sodium citrate; Glycerol;; IA (femoral);; rat;; 2002;; 14 days;; Controls received mp w/ vehicle; cardiovascular; peptides; vehicle was PBS w/ 10% sodium citrate and 1.6% glycerol; sodium citrate used to prevent coagulation; glycerol used to enhance protein stability;.

P4855 De Meyer,G.R.Y., Kockx,M.M., Cromheeke,K.M., Seye,C.I., Herman,A.G., Bult,H. Periadventitial inducible nitric oxide synthase expression and intimal thickening. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2000; 20(--):1896-1902. >>> Lysine, L-N6-(1-imino-ethyl)-; Saline; Polymyxin; IA (left carotid); rabbit; 2ML2; 14 days; controls received mp w/ vehicle; functionality of mp verified by immunostaining to verify iNOS activity; cardiovascular; enzyme inhibitor; Polymyxin B present in vehicle (2ug/ml) to bind all traces of lipopolysaccharides. L-NIL is a nitric oxide synthase inhibitor..

R0244 Gruber,S.A., Shirbacheh,M.V., Jones,J.W., Barker,J.H., Breidenbach,W.C. Local drug delivery to composite tissue allografts. MICROSURGERY 2000; 20(-8-):407-411. >>> Cyclosporin A; FK-506; IA (brachial); Rabbit; 6 days; Dose-response; review, see pg. 409.

P5238 Stevenson,K.M., Edgley,A.J., Bergstrom,G., Worthy,K., Kett,M.M., Anderson,W.P. Angiotensin II infused intrarenally causes preglomerular vascular changes and hypertension. Hypertension 2000/11; 36(-5-):839-844. >>> Angiotensin II; Saline; Heparin; IP; IA (renal artery); Rat; 2ML4; 14-25 days; Controls received mp w/ vehicle; cardiovascular; vehicle contained 10 IU/ml of heparin.

P4491 Yang,H.T, Feng,Y. bFGF increases collateral blood flow in aged rats with femoral artery ligation.. Am.J.Physiol.(Heart Circ.Physiol) 2000; 278(--):H85-H93. >>> Fibroblast growth factor, basic;; Sodium citrate; Glycerol; PBS;; IA (iliac);; rat;; 2002;; 14 days;; controls received mp w/ vehicle; dose response; peptides, cardiovascular; recomb. human FGF used; sodium citrate used to maintain catheter patency; glycerol used to stabilize protein..

P5303 Nichols,T.C., du,Laney T., Zheng,B., Bellinger,D.A., Nickols,G.A., Engleman,W., Clemmons,D.R. Reduction in atherosclerotic lesion size in pigs by alphaVbeta3 inhibitors is associated with inhibition of insulin-like growth factor- I-mediated signaling. Circ.Res 1999/11/26; 85(-11-):1040-1045. >>> SC-69000; SC-65811; IA (carotid, femoral); Pig; 2ML2; 14 days; Controls received mp w/ vehicle; cardiovascular; peptides; agents are aVb3 receptor inhibitors; diagram of pump placement on p.1041.

P4577 Shirbacheh,M.V, Harralson,T.A, Jones,J.W, Breidenbach,W.C, Jevans,A.W, Maldonado,C, Barker,J.H, Gruber,S.A  The Journal of Pharmacology and Experimental Therapeutics. 1999; 289(-3-):1191-1195. >>> Cyclosporin A; Cremophor; sandimmune IV solution; IA (brachial); Rabbit; 2ML1; 6 days; Dose-response (p. 1192-1194); immunology; sandimmune (CSA) solution used; intramedic PE-60/PE-10 infusion catheter used; to acheive highest dose; two pumps were implanted and catheters were joined via a Y-connector;.

P4576 Shirbacheh,M.V, Ren,X, Jones,J.W, Breidenbach,W.C, Jevans,A.W, Fernandez-Botran,G.R, Maldonado,C, Barker,J.H, Gruber,S.A  The Journal of Pharmacology and Experimental Therapeutics. 1999; 289(-3-):1185-1190. >>> Cyclosporin A; Sandimmune IV solution; IA (brachial); IV (jugular);; rabbit;; 2ML1;; 6 days; good surgical methods (p. 1186); immunology; diagram of pump/catheter system (p. 1186); PE-60 tubing was glued to PE-10 tubing; Sandimmune (CSA) solution used;.

P5285 Shirbacheh,M.V., Jones,J.W., Harralson,T.A., Edelstein,J., Tecimer,T., Breidenbach,W.C., Jevans,A.W., Maldonado,C., Barker,J.H., Gruber,S.A. Pharmacokinetics of intra-arterial delivery of tacrolimus to vascularly isolated rabbit forelimb. J Pharmacol Exp.Ther 1999/6; 289(-3-):1196-1201. >>> FK-506; IA (brachial); Rabbit; 2ML1; 6 days; Functionality of mp verified by FK-506 blood levels via ELISA; dose-response (p.1198); FK506 also known as tacrolimus; continuous systemic vs. local administration; immunology; transplantation.

P4004 Garin,E.H., Laflam,P., Chandler,L. Anti-interleukin 8 antibody abolishes effects of lipoid nephrosis cytokine. Pediatric Nephrology 1998; 12(--):381-385. >>> Cell culture supernatant factor; Antibody, anti-human interleukin 8; PBS, sterile; Albumin, bovine serum; IA (renal); rat; 2ML1; 5 days; controls received mp w/vehicle; functionality of mp verified by residual volume; heat-stretched PE-10 tubing used; immunology.

P4128 Hopkins,S.P., Bulgrin,J.P., Sims,R.L., Bowman,B., Donovan,D.L., Schmidt,S.P. Controlled delivery of vascular endothelial growth factor promotes neovascularization and maintains limb function in a rabbit model of ischemia. J.Vasc.Surg. 1998; 27(-5-):886-895. >>> Vascular endothelial growth factor; Nitroglycerin; Saline; Heparin; IA (iliac); rabbit; 2ML4; 28 days; pump diagram (p. 888); 22 g intravenous catheter was inserted into tygon tubing; "implantable osmotic pumps offer an alternative simple delivery method, providing a constant release of soluble agents in a steady-state fashion." (p. 892); controls received mp w/vehicle; good methods (pp. 887-888); cardiovascular; peptides; ischemia.

P4461 Yang,H.T, Ogilvie,R.W, Terjung,R.L  Am.J.Physiol.(Heart Circ.Physiol.43) 1998; 274(--):H2053-H2061. >>> Fibroblast growth factor, basic;; PBS; Sodium azide; Glycerol; Heparin;; IA (iliac);; rat;; 2002;; 2 weeks;; controls received mp w/vehicle; functionality of mp verified by residual volume; peptides; human. recomb fibroblast growth factor used;.

P3975 Cha,M.C., Rojhani,A. Zinc deficiency inhibits the direct growth effect of growth hormone on the tibia of hypophysectomized rats. Biol.Trace.Elem.Res. 1997; 59(--):99-111. >>> Growth hormone, recomb. human; IA (femoral); rat; 2002; 13 days; controls received mp w/vehicle; replacement therapy (hypophysectomy); no stress (see pg. 103); stress/adverse reaction (p. 103); PE10, PE50 and PE60 tubing used; PE10 tubing was stretched to fit into the vessel; peptides;.

P3740 Chen,C., Hanson,S.R., Keefer,L.K., Saavedra,J.E., Davies,K.M., Hutsell,T.C., Hughes,J.D., Ku,D.N., Lumsden,A.B. Boundary layer infusion of nitric oxide reduces early smooth muscle cell proliferation in the endarterectomized canine artery. J.Surg.Res. 1997; 67(--):26-32. >>> Proline, l-; Proline, nitric oxide-; NaOH; IA (femoral); dog; 2ML2; 7 days; controls received mp w/ proline to contralateral vessel; stability verified by in vitro assay; half-life (pg. 27); cardiovascular; constructed local infusion device and attached to mp via catheter.

P3730 Garin,E.H., West,L., Zheng,W. Effect of interleukin-8 on glomerular sulfated compounds and albuminuria. Pediatric Nephrology 1997; 11(--):274-279. >>> Interleukin-8; BSA; IA (renal); rat; 2ML1; 5 days; controls received mp w/BSA; good methods (pg. 275); peptides; used PE-10 catheter stretched to further reduce its diameter.

P3729 Ito,W.D., Arras,M., Winkler,B., Scholz,D., Schaper,J., Schaper,W. Monocyte chemotactic protein-1 increases collateral and peripheral conductance after femoral artery occlusion. Circ.Res. 1997; 80(--):829-837. >>> Uridine, bromodeoxy-; Monocyte chemoattractant protein-1; PBS; IA (femoral); Rabbit; 2ML2; no duration posted; controls received mp w/ PBS; functionality of mp verified by BrdU staining and residual volume; peptides; cardiovascular; MCP-1.

P4637 Matthys,K.E., Van Hove,C.E., Kockx,M.M, Andries,L.J., Osselaer,N.V, Herman,A.G., Bult,H  Arterioscler.Thromb.Vasc.Biol. 1997; 17(--):2423-2429. >>> Lipoprotein, low density-; lipoprotein, oxidized low density-;; PBS;; IA (carotid); rabbit; 14 days; controls received mp w/vehicle; pumps connected to silicone collars around carotid arteries; human LDL used..

P3530 Cardona-Sanclemente,L.E., Born,G.V.R. Increase by adrenaline or angiotensin II of the accumulation of low density lipoprotein and fibrinogen by aortic walls in unrestrained conscious rats. Br.J.Pharmacol. 1996; 117(--):1089-1094. >>> Epinephrine; Angiotensin II; Ascorbic acid; SC; IA (carotid); rat; 2ML4; 1007D; 6 days; controls received saline infusion; functionality of mp verified by plasma levels; stability verified by analyzing residual solution.

P3458 Lopez,J.J., Simons,M. Local extravascular growth factor delivery in myocardial ischemia. Drug Delivery 1996; 3(--):143-147. >>> Vascular endothelial growth factor; Heparin; Saline, sterile;; perivascular (circumflex artery adventitia); pig; 2ML2; 4 weeks; tissue perfusion (adventitia); peptides; pumps placed inside chest wall; cardiovascular; ischemia (cardiac).

P4428 Mattar,S.G, Hanson,S.R,Pierce G.F,Chen,C, Hughes,J.D, Cook,J,E, Shen,C, Noe,B.A, Suwyn,C.R, Scott,J.R, Lumsden,A.B  Journal of Surgical Research 1996; 60(--):339-344. >>> Fibroblast growth factor; saporin;; Sodium citrate; NaCl; EDTA;; IA (carotid);; dog;; 2002;; 14 days;; controls received mp w/vehicle; stability verified for 14 days by cellular assay; peptides; Saporin- FGF2 conjugate was infused; pump placed externally; Saporin is a potent ribosome-inactivating protein..

P4413 Yang,H.T, Deschenes,M.R, Ogilvie,R.W, Terjung,R.L  Circulation Research 1996/7; 79(-1-):62-69. >>> Fibroblast Growth Factor, basic; Heparin;; Saline; Glycerol; Sodium azide;; IA (Femoral);; rat;; 2002;; 1,2,4 weeks;; controls received mp w/ heparin; functionality of mp verified by residual volume; long-term study, pumps replaced at day 14; no stress (p. 63); good methods (p.63); peptides; cardiovascular;.

P3296 Garin,E.H  Pediatric Nephrology 1995; 9(--):587-593. >>> Cell cultures, supernatant fraction of PBM; IA (renal); rat; 2ML1; 5 days; controls received mp w/ BSA in Hank's solution; idiopathic minimal lesion nephrotic syndrome; patient mononuclear cells used; pumps sutured to abdominal wall; "...chronic infusion allowed us to avoid the sudden increase in intravascular volume observed during the acute infusion studies..." (p.590).

P4100 Hirko,M.K., Lin,P.H., Gosselin,C., Ren,D., Greisler,H.P. In vivo tissue distribution of fibroblast growth factor-1 after intraarterial delivery. ASAIO Journal 1995; 41(--):M630-M633. >>> Fibroblast growth factor; Heparin; 125I tracer; Radio-isotopes; IA (carotid); rabbit; 2001D; 24 hours; functionality of mp verified by residual radioactivity analysis; no stress (see pg. M631); good methods (p. M631); peptides; tissue distribution.

P3381 Kirkham,T.C., Gibbs,J., Smith,G.P., Geary,N. Meal pattern analysis in rats reveals partial agonist activity of the bombesin receptor antagonist BW2258U89. Pharmacol.Biochem.Behav. 1995; 52(-1-):101-106. >>> BW2258U89; Saline; IA (celiac); rat; 2001; 6 days; controls received laporotomy&subsequent anesthesia; catheter tips passed through gauze before placement in arteries; catheters were anchored w/polypropylene microsuture; gauze was sutured to arterial wall.

P4096 Levy,A.E., Alexander,J.W. Administration of intragraft interleukin-4 prolongs cardiac allograft survival in rats treated with donor-specific transfusion/cyclosporine. Transplantation 1995; 60(-5-):405-406. >>> Interleukin-4; Interleukin-10; IA (brachiocephalic); rat; 14 days; immunology; peptides; pump infused the brachiocephalic artery of a harvested heart, which was then implanted into a recipient; recomb. mouse IL-4&IL-10 used.

P3404 Yoshimura,N., Lee,C.J., Shiho,O., Kita,M., Imanishi,J., Oka,T. Local immunosuppressive therapy with monoclonal anti-T-cell antibody on renal allograft survival in the rat. II. Phenotypic and functional assessment of spleen cells. Transplant.Proc. 1995; 27(-1-):390-391. >>> Antibody, monoclonal, OX-19; Saline; IA (renal); IV (femoral); rat; 7 days; immunology; arterial infusion proved more effective than venous infusion.

P2290 Andrews,T.J., Cowen,T. In vivo infusion of NGF induces the organotypic regrowth of perivascular nerves following their atrophy in aged rats. J.Neurosci. 1994; 14(-5-):3048-3058. >>> Nerve growth factor; Albumin; Cytochrome C; PBS; perivascular (cerebral artery adventitia); rat; 2002; 14 days; controls received infusion of vehicle only; tissue perfusion (arterial wall); peptides; nerve growth observed in control group also, see p. 3057; no stress (p. 3049).

P2617 Baskin,D.S., Browning,J.L., Widmayer,M.A., Zhu,Z.-Q., Grossman,R.G. Development of a model for Parkinson's disease in sheep using unilateral intracarotid injection of MPTP via slow continuous infusion. Life Sci. 1994; 54(-7-):471-479. >>> MPTP HCl; IA (carotid); sheep; 2ML1; 1-7 weeks; Neurodegenerative (Parkinson's disease); comparison of acute injections vs. mp; no stress (see pg. 474); catheter placed in proximal occipital artery (w/ distal end of artery occluded); ". . .slow continuous infusion may be a useful alternative to repeated dosing in animal studies using MPTP. . .".

P2526 Alarid,E.T., Chen,P., Schaudies,R.P., Nicoll,C.S. Differential effects of an antiserum to epidermal growth factor on the development of transplanted rat embryos and fetal structures in vivo. Growth Factors 1993; 8(--):235-243. >>> Antibody, anti-epidermal growth factor; Epidermal growth factor; Water; Glycerol; Sodium nitrate; Heparin; Albumin; IA (renal); rat; 2002; 14 days; tissue perfusion (fetal tissue transplants); peptides.

P2220 Chiang,M.H., Kelley,K.M., Nicoll,C.S. Human placental lactogen directly inhibits rat cartilage growth processes in vivo and in vitro. Acta Endocrinol. 1993; 128(--):65-68. >>> Human placental lactogen; IA (iliac); rat; 2001; 7 days; controls received mp w/vehicle; stability verified in vitro for 6 days; peptides.

P2982 Ferraresso,M., Rigotti,P., Stepkowski,S.M., Chou,T.-C., Kahan,B.D. Immunosuppressive effects of defibrotide. Transplantation 1993; 56(-4-):928-933. >>> Defibrotide; Cyclosporin A; PBS; Cremophor; IA (innominate); IV (lumbar); rat; 2002; 2ML1; no duration posted; Controls were untreated; comparison of ip injections or oral gavage vs. mp; defibrotide infused into artery of graft or recipient vein; defibrotide is an immunosuppressant w/ antithrombotic and profibrinolytic activities.

P2511 Lee,C.J., Yoshimura,N., Shiho,O., Kita,M., Oka,T. Local immunosuppressive therapy with monoclonal anti-T cell antibody on renal allograft survival in the rat. Clin Exp Immunol 1993; 91(--):362-367. >>> Antibody, monoclonal; Saline; IA (renal); IV (femoral); rat; 2ML1; 7 days; tissue perfusion (kidney allograft); dose-response (table, p.364); immunology; comparison of single IV bolus, systemic IV infusion, or regional IA infusion.

P2525 Moses,B.L., Chan,D.W., Hruban,R.H., Forastiere,A., Richtsmeier,W.J. Comparison of intra-arterial and intravenous infusion of cisplatin for head and neck squamous cell carcinoma in a modified rat model. Arch Otolaryngol.Head Neck Surg. 1993; 119(--):612-617. >>> Cisplatin; IV (saphenous); IA (saphenous); rat (nude); 2ML1; 6 days; tissue perfusion (tumor xenograft); cancer.

P2099 Alarid,E.T., Schlechter,N.L., Russell,S.M., Nicoll,C.S. Evidence suggesting that insulin-like growth factor-I is necessary for the trophic effect of insulin on cartilage growth in vivo. Endocrinology 1992; 139(-4-):2305-2309. >>> Antibody, anti-IGF I; Insulin; 125I tracer; Heparin; Radio-isotopes; Serum, rabbit; IA (iliac); rat; 2001; 7 days; functionality of mp verified by RIA; dose-response; peptides.

P2154 Matejka,G.L., Jennische,E. Local infusion of IGF-I into the kidney of pituitary intact rats induces renal growth. Acta Physiol.Scand. 1992; 145(--):7-18. >>> Insulin-like growth factor I; Albumin, bovine serum; Saline; IA (suprarenal); rat; 2ML1; 7 days; controls received mp with vehicle; tissue perfusion (kidney); dose-response; peptides; pumps infused into the artery and directly into renal parenchyma via a catheter sealed at the end and punctured along the sides; recomb. human IGF-1 used.

P2089 Riemenschneider,T., Ruf,C., Kratzsch,H.C., Ziegler,M., Spath,G. Arterial, portal or combined arterio-portal regional chemotherapy in experimental liver tumours?. J.Cancer Res.Clin.Oncol. 1992; 118(--):596-600. >>> Uridine, fluorodeoxy-; Heparin; Saline; IA (hepatic); IV (hepatic portal); rat; 2ML2; 7 days; Cancer; 5000 IU/kg of heparin dissolved in saline; animal info (SD, F, 120-150 g).

P2091 Riemenschneider,Th., Ruf,C., Kratzsch,H.C., Ziegler,M., Spath,G. Arterial, portal or combined arterio-portal regional chemotherapy in experimental liver tumours. J.Cancer Res.Clin.Oncol. 1992; 118(--):597-600. >>> Uridine, fluorodeoxy-; Heparin; Saline; IA (hepatic); IV (hepatic portal); rat; 2ML2; 7 days; tissue perfusion (tumor); cancer; hepatic artery accessed via gastroduodenal artery; portal vein via ileocolic vein; animal info (SD, F, 120-150 g).

P1890 Alarid,E.T., Cunha,G.R., Young,P., Nicoll,C.S. Evidence for an organ- and sex-specific role of basic fibroblast growth factor in the development of the fetal mammalian reproductive tract. Endocrinology 1991; 129(-4-):2148-2154. >>> Antibody, anti-fibroblast growth factor; Glycerol; Sodium azide; IA (suprarenal); rat; 2002; 14 days; tissue perfusion (graft); peptides.

P2594 Andersen,D.E., Reid,S.D., Moon,T.W., Perry,S.F. Metabolic effects associated with chronically elevated cortisol in rainbow trout (Oncorhynchus mykiss). Can.J.Fish.Aquat.Sci. 1991; 48(-9-):1811-1817. >>> Cortisol; Cyclodextrin, B-; IA (dorsal aorta); fish (rainbow trout); 2001; 10-14 days; controls received mp w/ vehicle or sham operation; functionality of mp verified by RIA of plasma levels; stress from surgery caused hyperglycemia (p.816) for 22 hours; "Mini-osmotic pumps. . .were an effective method for chronically elevating cortisol titers in trout."; Molecusol HBP is a beta-cyclodextrin.

R0084 Daemen,M.J.A.P., Thijssen,H.H.W., Struyker-Boudier,H.A.J. Pharmacokinetic considerations in local drug delivery. Adv.Drug Delivery Review 1991; 6(--):1-18. >>> Hippuric acid; 125I tracer; Radio-isotopes; IA (suprarenal); rat; no duration posted; tissue perfusion.

P1946 Kamei,T., Callery,M.P., Flye,M.W. Intragraft delivery of 16, 16-dimethyl PGE2 induces donor-specific tolerance in rat cardiac allograft recipients. Transplantation 1991; 51(-1-):242-246. >>> Prostaglandin E2 analogue; Ethanol; Saline; IA (innominate); IP; IV (lumbar); rat; 2ML1; 2ML2; 1, 2 weeks; tissue perfusion (cardiac allograft); immunology; pumps implanted IP.

P2015 Stepkowski,S.M., Chen,H., Daloze,P., Kahan,B.D. Rapamycin, a potent immunosuppressive drug for vascularized heart, kidney, and small bowel transplantation in the rat. Transplantation 1991; 51(-1-):22-26. >>> Rapamycin; Dimethylacetamide; PEG 400; Tween 80; IA (innominate); IV (lumbar); rat; 2002; 14 days; tissue perfusion (allograft).

P1690 Liu,L., Russell,S.M., Nicoll,C.S. Analysis of the role of basic fibroblast growth factor in growth and differentiation of transplanted fetal rat paws and intestines. Endocrinology 1990; 126(-3-):1764-1770. >>> Antibody, anti-fibroblast growth factor; Fibroblast growth factor; Glycerol; Heparin; Saline; Sodium azide; Sodium citrate; IA (renal); rat; 2001; 2002; 6-11 days; tissue perfusion: tissue graft; good methods for FGF/pg. 1765; peptides; basic FGF used.

P3291 Norman J., Tsau,Y., Bacay,A., Fine,L.G. Epidermal growth factor accelerates functional recovery from ischaemic acute tubular necrosis in the rat: role of the epidermal growth factor receptor. Clin.Sci. 1990; 78(--):445-450. >>> Epidermal growth factor; Saline; IA (renal); rat; 2001; 8 days; controls received mp w/saline; peptides; medical category: renal; skc.