References on the Intracerebroventricular Administration of Agents Using ALZET^® Osmotic Pumps

R0247 Fernstrom,J.D., Choi,S. The development of tolerance to drugs that suppress food intake. PHARMACOLOGY&THERAPEUTICS 2008; 117(-1-):105-122. >>> Fenfluramine; leptin; melanotan II; CSF, artificial; PBS; SC; CSF/CNS; Rat; 8 weeks; 7, 28 days; Controls received mp w/ vehicle; dose-response (fig. 5); pumps replaced; tolerance; peptides; animal info (male, obese); endocrinology; review, see p. 108-110.

R0246 Hedlund,E., Hefferan,M.P., Marsala,M., Isacson,O. Cell therapy and stem cells in animal models of motor neuron disorders. European Journal of Neuroscience 2007; 26(-7-):1721-1737. >>> Vascular endothelial growth factor; CSF/CNS; Rat; ; Animal info (SOD1G93A, P60, P85); neurogenerative (ALS); Review; see p. 1722-1724, 1726-1727; ref. Storkebaum et al, 2005.

R0240 Bazan,N.G. Omega-3 fatty acids, pro-inflammatory signaling and neuroprotection. CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE 2007; 10(-2-):136-141. >>> Neuroprotectin D1; CSF/CNS (third ventricle); Mice; ; Ischemia (cerebral); neurodegenerative (Alzheimer's disease); see pg. 137.

R0233 Monnet,F.P., Maurice,T. The sigma1 protein as a target for the non-genomic effects of neuro (active) steroids: Molecular, physiological, and behavioral aspects. JOURNAL OF PHARMACOLOGICAL SCIENCES 2006; 100(-2-):93-118. >>> Amyloid protein, beta (1-40); CSF/CNS; Rat; 14 days; Neurodegenerative (alzheimer's disease); see pg. 111, ref. 29.

P9195 Patzer,A., Zhao,Y., Stoeck,I., Gohlke,P., Herdegen,T., Culman,J. Peroxisome proliferator-activated receptors gamma; (PPAR gamma) differently modulate the interleukin-6 expression in the peri-infarct cortical tissue in the acute and delayed phases of cerebral ischaemia. European Journal of Neuroscience 2008; 28(-9-):1786-1794. >>> Pioglitazone; DMSO; saline; CSF/CNS; Rat; 2002; 6 or 7 days; Controls received mp w/ vehicle; animal info (male, normotensive, Wistar, MCAO, 220-250 g.); 50% DMSO used; middle cerebral artery occlusion; ischemia (cerebral).

P9192 Singh,M.K., Elefteriou,F., Karsenty,G. Cocaine and amphetamine-regulated transcript may regulate bone remodeling as a circulating molecule. Endocrinology 2008; 149(-8-):3933-3941. >>> CART; PBS; CSF/CNS (third ventricle); Mice (transgenic); 28 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; cyanoacrylate adhesive; animal info (CART -/-, female, 5 months old); compound also known as cocaine and amphetamine-regulated transcript, a mediator of the leptin regulation of bone mass.

P9189 Gersting,J., Schaub,C.E., Keller-Wood,M., Wood,C.E. Inhibition of brain prostaglandin endoperoxide synthase-2 prevents the preparturient increase in fetal adrenocorticotropin secretion in the sheep fetus. Endocrinology 2008; 149(-8-):4128-4136. >>> Nimesulide; DMSO; water; CSF/CNS; Sheep; 2ML2; 2ML4; ; Controls received mp w/ vehicle; post op. care (ampicillin); animal info (ewe, fetus, 119-125 d gestation); nimesulide is a COX-2 selective NSAID; incisions were sutured; 50% DMSO used.

P9182 Alonso,G., Gallibert,E., Lafont,C., Guillon,G. Intrahypothalamic angiogenesis induced by osmotic stimuli correlates with local hypoxia: A potential role of confined vasoconstriction induced by dendritic secretion of vasopressin. Endocrinology 2008; 149(-9-):4279-4288. >>> Vasopressin, 1-deamino-8-D-arginine; CSF/CNS; Rat; 1007D; 3, 6 days; Controls received mp w/ PBS; animal info (male, Wistar, Long Evans); dAVP; 26 gauge 10 mm cannula used.

P9178 Vazquez,M.J., Gonzalez,C.R., Varela,L., Lage,R., Tovar,S., Sangiao-Alvarellos,S., Williams,L.M., Vidal-Puig,A., Nogueiras,R., Lopez,M., Dieguez,C. Central resistin regulates hypothalamic and peripheral lipid metabolism in a nutritional-dependent fashion. Endocrinology 2008; 149(-9-):4534-4543. >>> Resistin; Saline; CSF/CNS; Rat; 2001D; 2ML2; 6 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 300-350 g.); incision was closed with sutures; cannula placement confirmed by methylene blue staining.

P9177 Holmberg,E., Zhang,S.X., Sarmiere,P.D., Kluge,B.R., White,J.T., Doolen,S. Statins decrease chondroitin sulfate proteoglycan expression and acute astrocyte activation in central nervous system injury. Experimental Neurology 2008; 214(-1-):78-86. >>> Simvastatin; CSF/CNS; Rat; 2004; 4 weeks; Comparison of PO gavage vs. mp; ALZET brain infusion kit used; post op. care (buprenorphine); animal info (female, Long Evans, adult, 200-22 g.); cannula placement confirmed by preparing cryosections of the brain at the lateral ventricle and verifying the presence of a cavity created by the cannula; spinal cord injury.

P9176 Knauf,C., Cani,P.D., it-Belgnaoui,A., Benani,A., Dray,C., Cabou,C., Colom,A., Uldry,M., Rastrelli,S., Sabatier,E., Godet,N., Waget,A., Penicaud,L., Valet,P., Burcelin,R. Brain glucagon-like peptide 1 signaling controls the onset of high-fat diet-induced insulin resistance and reduces energy expenditure. Endocrinology 2008; 149(-10-):4768-4777. >>> Exendin-9; SC; CSF/CNS; Mice; 2004; 4 weeks; Controls received SC mp w/ Exendin-9; animal info (C57BL6/J, 12 wks old).

P9174 Trotter-Mayo,R.N., Roberts,M.R. Leptin acts in the periphery to protect thymocytes from glucocorticoid-mediated apoptosis in the absence of weight loss. Endocrinology 2008; 149(-10-):5209-5218. >>> Leptin; IP; CSF/CNS; Mice; 1002; 12 days; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ob/ob, db/db, wt, C57BL/6).

P9167 Bretzner,F., Liu,J., Currie,E., Roskams,A.J., Tetzlaff,W. Undesired effects of a combinatorial treatment for spinal cord injury - transplantation of olfactory ensheathing cells and BDNF infusion to the red nucleus. European Journal of Neuroscience 2008; 28(-9-):1795-1807. >>> Brain-derived neurotrophic factor; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 300-400 g.); 28 gauge cannula, silastic tubing used; spinal cord injury; wound clips used.

P9164 Sud,R., Spengler,R.N., Nader,N.D., Ignatowski,T.A. Antinociception occurs with a reversal in alpha2-adrenoceptor regulation of TNF production by peripheral monocytes/macrophages from pro- to anti-inflammatory. European Journal of Pharmacology 2008; 588(-2-3-):217-231. >>> Tumor necrosis factor, recomb. rat; CSF, artificial; CSF/CNS; Rat; 2002; ; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male, Sprague Dawley, 200-300 g.); gentamycin was added to the aCSF to prevent bacterial growth; rat albumin was added to stabilize the infused compound.

P9146 Zhang,Z.H., Yu,Y., Kang,Y.M., Wei,S.G., Felder,R.B. Aldosterone acts centrally to increase brain renin-angiotensin system activity and oxidative stress in normal rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-2-):H1067-H1074. >>> Aldosterone; RU-28318; Tempol; losartan; CSF, artificial; CSF/CNS; Rat; 2002; 1 week; Controls received mp w/ vehicle; post op. care (buprenorphine); animal info (male, Sprague Dawley, 250-300 g.); silastic tubing used; dental cement used.

P9145 Kang,Y.M., Zhang,Z.H., Xue,B.J., Weiss,R.M., Felder,R.B. Inhibition of brain proinflammatory cytokine synthesis reduces hypothalamic excitation in rats with ischemia-induced heart failure. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-1-):H227-H236. >>> Pentoxifylline; CSF, artificial; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 275-300 g.).

P9138 Berhanu,D.A., Rush,R.A. Targeted silencing of TrkA expression in rat forebrain neurons via the p75 receptor. Neuroscience 2008; 153(-4-):1115-1125. >>> Immunoglobulin G, TrkA, inhibitor; immunoglobulin G, TrkA, scrambled; CSF/CNS (third ventricle); Rat; 2004; 7, 20 days; Controls received mp w/ TrkAm-fk Abp75; animal info (Sprague Dawley, adult, 270-300 g.); behavioral testing (Morris water maze apparatus).

P9120 Shen,Q., Wang,Y., Kokovay,E., Lin,G., Chuang,S.M., Goderie,S.K., Roysam,B., Temple,S. Adult SVZ stem cells lie in a vascular niche: A quantitative analysis of niche cell-cell interactions. Cell Stem Cell 2008; 3(-3-):289-300. >>> Antibody, GoH3; CSF, artificial; CSF/CNS; Mice (transgenic); 1007D; 6 days; Controls received mp w/ immunoglobulin G, rat; animal info (2-3 months old, Swiss Webster, GFAP-GFP).

P9118 Knauf,C., Cani,P.D., Kim,D.H., Iglesias,M.A., Chabo,C., Waget,A., Colom,A., Rastrelli,S., Delzenne,N.M., Drucker,D.J., Seeley,R.J., Burcelin,R. Role of central nervous system glueagon-like peptide-1 reeeptors in enterie glueose sensing. Diabetes 2008; 57(-10-):2603-2612. >>> Exendin (9-39); CSF/CNS; Mice; ; ALZET brain infusion kit; animal info (male, C57BL/6, 12 wks old, GLP-1Rc KO); cannula connected to tygon tubing filled with artificial CSF; dental cement used.

P9104 Monkkonen,K.S., Hakumaki,J.M., Hirst,R.A., Miettinen,R.A., O'Callaghan,C., Mannisto,P.T., Laitinen,J.T. Intracerebroventricular antisense knockdown of G alphai2 results in ciliary stasis and ventricular dilatation in the rat. BMC.Neurosci. 2007; 8(--):26-. >>> Oligodeoxynucleotide, antisense; oligodeoxynucleotide, nonsense; oligodeoxynucleotide, mismatch; Saline, sterile; CSF/CNS; Rat; 1007D; 2002; 7 days; Controls received mp w/ vehicle; no stress (see pg. 2 of 15); MRI; animal info (male, Harlan Wistar, 3-5 wks old, 50-100 g.; adult, 250 g.).

P9076 Liu,D.M., Gao,L., Roy,S.K., Cornish,K.G., Zucker,I.H. Role of oxidant stress on AT1 receptor expression in neurons of rabbits with heart failure and in cultured neurons. Circulation Research 2008; 103(-2-):186-193. >>> Tempol; CSF, artificial; CSF/CNS; Rabbit; 2001; 7 days; Controls received mp w/ vehicle; animal info (New Zealand, White, 3.5-4.3 kg.); 19 gauge cannula used.

P9074 Xue,B.J., Zhao,Y.Z., Johnson,A.K., Hay,M. Central estrogen inhibition of angiotensin II-induced hypertension in male mice and the role of reactive oxygen species. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1025-H1032. >>> Tempol; estradiol, 17B-; ICI-182780; SC; CSF/CNS; Mice; 1002; ; ALZET brain infusion kit used; peptides; animal info (male, C57BL/6, 12-16 wks old); location of cannula was verified histologically post mortem.

P9059 Yang,D.S., Kumar,A., Stavrides,P., Peterson,J., Peterhoff,C.M., Pawlik,M., Levy,E., Cataldo,A.M., Nixon,R.A. Neuronal apoptosis and autophagy cross talk in aging PS/APP mice, a model of Alzheimer's disease. American Journal of Pathology 2008; 173(-3-):665-681. >>> Leupeptin; HEPES, buffer; CSF/CNS; Mice (transgenic); 2004; 2 weeks; Enzyme inhibitor (cysteine protease); cyanoacrylate adhesive; animal info (6 months old, PS/APP, or wt); neurodegenerative (Alzheimer's Disease); pumps primed in sterile saline at 37 degree Celsius for 48 hours.

P9054 Hao,Q., Su,H., Marchuk,D.A., Rola,R., Wang,Y.Q., Liu,W.Z., Young,W.L., Yang,G.Y. Increased tissue perfusion promotes capillary dysplasia in the ALK1-deficient mouse brain following VEGF stimulation. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-6-):H2250-H2256. >>> Hydralazine; nicardipine; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ saline; animal info (adult, male, ALK1 +/-); fig. 1 illustrates cannula placement; cannula placement verified by CBF measurements.

P9053 Kang,Y.M., Ma,Y., Elks,C., Zheng,J.P., Yang,Z.M., Francis,J. Cross-talk between cytokines and renin-angiotensin in hypothalamic paraventricular nucleus in heart failure: role of nuclear factor-kappa B. Cardiovascular Research 2008; 79(-4-):671-678. >>> Losartan; SN50; tempol; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; peptides; animal info (male, Sprague Dawley, 250-275 g.); pumps were primed in 0.9% saline for 24 hours; SN50 is a synthetic protein.

P9007 Hillebrand,J.J.G., Kas,M.J.H., Scheurink,A.J.W., van Dijk,G., Adan,R.A.H. AgRP(83-132) and SHU9119 differently affect activity-based anorexia. European Neuropsychopharmacology 2006; 16(-6-):403-412. >>> SHU 9119; agouti-related protein (83-132), human; Saline, sterile, isotonic; CSF/CNS; Rat; 1007D; 4, 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; post op. care (Buprenorphine, temagesic); animal info (female, Wistar wu); melanocortin antagonist.

P8999 Sagara,Y., Hirooka,Y., Nozoe,M., Ito,K., Kimura,Y., Sunagawa,K. Pressor response induced by central angiotensin II is mediated by activation of Rho/Rho-kinase pathway via AT1 receptors. Journal of Hypertension 2007; 25(-2-):399-406. >>> Angiotensin II; phenylephrine; Y-27632; valsartan; CSF, artificial; SC; CSF/CNS; Rat; 1002; 2001; 7 days; Controls received mp w/ vehicle; enzyme inhibitor (Rho-kinase); antihypertensive; peptides; animal info (male, Wistar-Kyoto, SHR, 280-340 g.); catheter tip placement and connection confirmed.

P8997 Zhao,Y., Patzer,A., Herdegen,T., Gohlke,P., Culman,J. Activation of cerebral peroxisome proliferator-activated receptors gamma promotes neuroprotection by attenuation of neuronal cyclooxygenase-2 overexpression after focal cerebral ischemia in rats. FASEB Journal 2006; 20(-8-):1162-1175. >>> Pioglitazone; PBS; DMSO; CSF/CNS; Rat; 2002; 7-8 days; Controls received mp w/ vehicle; ischemia (cerebral); animal info (male, Wistar, 200-220 g., MCAO); 25% DMSO used; "since pioglitazone reaches very low concentrations in the brain soon after its systemic application, a long-term ICV infusion of pioglitazone was chose to ensure an effective activiation of brain PPARg and to exclude any contribution of peripheral effects of the PPARg ligand." (p. 1167).

P8990 Thored,P., Arvidsson,A., Cacci,E., Ahlenius,H., Kallur,T., Darsalia,V., Ekdahl,C.T., Kokaia,Z., Lindvall,O. Persistent production of neurons from adult brain stem cells during recovery after stroke. Stem Cells 2006; 24(-3-):739-747. >>> AMD3100; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; brain tissue distribution; ischemia (cerebral); animal info (male, Wistar, MCAO); CXCR4 antagonist; behavioral testing (horizontal ladder walking test, stepping test, cylinder test).

P8988 Wachs,F.P., Winner,B., Couillard-Despres,S., Schiller,T., Aigner,R., Winkler,J., Bogdahn,U., Aigner,L. Transforming growth factor-beta 1 is a negative modulator of adult neurogenesis. Journal of Neuropathology and Experimental Neurology 2006; 65(-4-):358-370. >>> Transforming growth factor-B1, recomb.; CSF, artificial; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; peptides; animal info (female, Fischer-344, 2-3 months old, 180 g.).

P8976 Park,S.W., Yan,Y.P., Satriotomo,I., Vemuganti,R., Dempsey,R.J. Substance P is a promoter of adult neural progenitor cell proliferation under normal and ischemic conditions. Journal of Neurosurgery 2007; 107(-3-):593-599. >>> Substance P; L703,606; CSF, artificial; CSF/CNS; Rat; 2001; 5 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; peptides; ischemia (cerebral); animal info (male, SHR, MCAO, 250-350 g.); NK1R antagonist.

P8971 Jang,S., Kim,H., Kim,D., Jeong,M.W., Ma,T., Kim,S., Ho,I.K., Oh,S. Attenuation of morphine tolerance and withdrawal syndrome by coadministration of nalbuphine. ARCHIVES OF PHARMACAL RESEARCH 2006; 29(-8-):677-684. >>> Morphine chloride; nalbuphine HCl; Saline; CSF/CNS; Rat; 2001; 3 days; Controls received mp w/ vehicle; comparison of IP injections vs. mp; tolerance; dependence; animal info (male, Sprague Dawley, 220-240 g.).

P8968 Zeinoaldin,S., Swarts,J.J.M., Van de Heijning,B.J.M. Central application of IGF-1 postpones time of vaginal opening in normally fed, but not in food-restricted rats. Hormone Research 2006; 66(-4-):169-174. >>> Insulin-like growth factor-1, recomb. human; Acetic acid; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ saline; peptides; animal info (Wistar, female, 23 days old, 45 g.); cannula position confirmed with Evans blue dye post mortem; endocrinology.

P8967 Knight,W.D., Swoap,S.J., Parsons,A.D., Overton,J.M. Central thyrotropin-releasing hormone infusion opposes cardiovascular and metabolic suppression during caloric restriction. Neuroendocrinology 2006; 83(-2-):69-76. >>> Thyrotropin-releasing hormone; PBS; CSF/CNS; Rat; 1002; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, Sprague Dawley, 350 g.); behavioral testing (locomotor activity).

P8954 Teruyama,R., Lipschitz,D.L., Wang,L., Ramoz,G.R., Crowley,W.R., Bealer,S.L., Armstrong,W.E. Central blockade of oxytocin receptors during mid-late gestation reduces amplitude of slow afterhyperpolarization in supraoptic oxytocin neurons. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 295(-5-):E1167-E1171. >>> desGly-NH2-d(CH2)5[D-Tyr2, Thr4]OVT; CSF, artificial; CSF/CNS (third ventricle); Rat (pregnant); 2002; 7-8 days; Controls received mp w/ vehicle; good methods (p. E1168); animal info (Spgraue Dawley); polyethylene tubing used; compound is an oxytocin receptor antagonist (OTA).

P8952 Dolev,I., Michaelson,D.M. The nucleation growth and reversibility of Amyloid-beta deposition in vivo. JOURNAL OF ALZHEIMERS DISEASE 2006; 10(-2-3-):291-301. >>> Thiorphan, DL-; CSF, artificial; ascorbic acid; CSF/CNS; Mice (transgenic); 2004; 4 weeks; Controls received mp w/ vehicle; enzyme inhibitor (neprilysin); animal info (male, C57BL/6, ApoE3 tg, ApoE4 tg, wt, 5 months old); neurodegenerative (Alzheimer's disease).

P8951 Hook,V., Kindy,M., Hook,G. Cysteine protease inhibitors effectively reduce in vivo levels of brain beta-amyloid related to Alzheimer's disease. BIOLOGICAL CHEMISTRY 2007; 388(-2-):247-252. >>> CAO74Me; E64d; Saline; DMSO; CSF/CNS; Guinea-pig; 2ML4; 7, 30 days; Controls received mp w/ vehicle; no stress (see pg. 250); enzyme inhibitor (cysteine protease, cathepsin B); animal info (Harley, 300-500g, 5-7 wks old); neurodegenerative (Alzheimer's disease); 1.5% DMSO used; "Animals treated with E64d and CAO74Me appeared healthy without any signs of toxicity, and normal behavior was observed." (p. 250).

P8941 Komatsu,T., Chiba,T., Yamaza,H., To,K., Toyama,H., Higami,Y., Shimokawa,I. Effect of leptin on hypothalamic gene expression in calorie-restricted rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 2006; 61(-9-):890-898. >>> Leptin, recomb.; PBS; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Fischer 344, 34 wks old).

P8934 Lu,J., Zhang,Y.D., Shi,J.P. Effects of intracerebroventricular infusion of angiotensin-(1-7) on bradykinin formation and the kinin receptor expression after focal cerebral ischemia-reperfusion in rats. Brain Research 2008; 1219(-;-):127-135. >>> Angiotensin (1-7); CSF, artificial; CSF/CNS; Rat; 1007D; ; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptide; ischemia (cerebral); animal info (male, Sprague Dawley, 280-300 g.).

P8932 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor-I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; 3 days; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.

P8917 Bartolomucci,A., Rigamonti,A.E., Bulgarelli,I., Torsello,A., Locatelli,V., Pavone,F., Levi,A., Possenti,R., Muller,E.E., Moles,A. Chronic intracerebroventricular TLQP-21 delivery does not modulate the GH/IGF-1-axis and muscle strength in mice. GROWTH HORMONE&IGF RESEARCH 2007; 17(-4-):342-345. >>> TLQP-21, synthetic; CSF, artificial; CSF/CNS; Mice; 1002; 14 days; ALZET brain infusion kit 1 used; peptides; animal info (male, Swiss CD1, 30-35 g.); behavioral testing (grip strength).

P8915 Lujan,M.E., Krzemien,A.A., Reid,R.L., Van Vugt,D.A. Effect of leptin administration on ovulation in food-restricted rhesus monkeys. Neuroendocrinology 2006; 84(-2-):103-114. >>> Leptin, recomb. human; Water, sterile; CSF/CNS; Monkey; 2002; 32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 wks; stress/adverse reaction: (see pg. 104) 1 animal removed from study due to CNS infection at 28 weeks; peptides; animal info (female, Rhesus, 6-10 yrs. old, 5-12 kg.); endocrinology.

P8912 Huang,B.S., White,R.A., Ahmad,M., Jeng,A.Y., Leenen,F.H.H. Central infusion of aldosterone synthase inhibitor prevents sympathetic hyperactivity and hypertension by central Na+ in Wistar rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-1-):R166-R172. >>> FAD286; CSF, artificial; CSF/CNS; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; enzyme inhibitor (aldosterone synthase); animal info (male, Wistar, 200-250 g.); dental cement used.

P8911 Sibilia,V., Bresciani,E., Lattuada,N., Rapetti,D., Locatelli,V., De Luca,V., Dona,F., Netti,C., Torsello,A., Guidobono,F. Intracerebroventricular acute and chronic administration of oblestatin minimally affect food intake but not weight gain in the rat. Journal of Endocrinological Investigation 2006; 29(-11-):RC31-RC34. >>> Obestatin; Water, distilled; saline, sterile; CSF/CNS; Rat; 2004; 28 days; Half life (p. RC-33) "rapid"; ALZET brain infusion kit used; animal info (male, Sprague Dawley, 7 wks old, 175-200 g.); peptides; obestatin is a 23 amino-acid peptides; Evans blue was injected through the cannula to confirm its position in the ventricle.

P8910 do Carmo,J.M., Hall,J.E., da Silva,A.A. Chronic central leptin infusion restores cardiac sympathetic-vagal balance and baroreflex sensitivity in diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-5-):H1974-H1981. >>> Leptin; CSF/CNS; Rat; 1007D; 7 days; Peptides; animal info (male, Sprague Dawley, 340-360 g.); tygon tubing used; diabetes.

P8906 Todd,B.J., Fraley,G.S., Peck,A.C., Schwartz,G.J., Etgen,A.M. Central insulin-like growth factor 1 receptors play distinct roles in the control of reproduction, food intake, and body weight in female rats. Biology of Reproduction 2007; 77(-3-):492-503. >>> JB-1; insulin-like growth factor-1, human; CSF, artificial; CSF/CNS; Rat; 1007D; 2002; 5, 7, 14 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 494) surgery led to a 10-15% loss of presurgical body weight, temporary disruption of estrous cycles; peptides; animal info (female, Sprague Dawley, 170-190 g.); IGF-1 receptor antagonist; cannula placement verified by dye injection at end of experiment; endocrinology.

P8900 Torregrosa,G., Perez-Asensio,F.J., Burguete,M.C., Castello-Ruiz,M., Salom,J.B., Alborch,E. Chronic intracerebroventricular delivery of the secretory phospholipase A(2) inhibitor, 12-epi-scalaradial, does not improve outcome after focal cerebral ischemia-reperfusion in rats. Experimental Brain Research 2007; 176(-2-):248-259. >>> Scalaradial, 12-epi-; Ethanol; cyclodextrin, 2-hydroxypropyl-B-; CSF/CNS; Rat; 2001; 4 days; Controls received mp w/ vehicle; dose-response (fig. 2); enzyme inhibitor (secretory phospholipase A2); ALZET brain infusion kit used; ischemia (cerebral); animal info (male, Wistar, 300-350 g., MCAO); 10% ethanol used; behavioral testing (locomotor activity, parachute reflex, forepaw stretching resistance).

P8893 Brenneman,M.M., Wagner,S.J., Cheatwood,J.L., Heldt,S.A., Corwin,J.V., Reep,R.L., Kartje,G.L., Mir,A.K., Schwab,M.E. Nogo-A inhibition induces recovery from neglect in rats. Behavioural Brain Research 2008; 187(-2-):262-272. >>> Antibody, anti-Nogo-A monoclonal 7B12; antibody, anti-Nogo-A monoclonal 11C7; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ control IgG antibody; no stress (see pg. 264); good methods (pg. 264); post op. care ("clean, warm environment"); pumps removed after 21 days.

P8882 Rich,N., Reyes,P., Reap,L., Goswami,R., Fraley,G.S. Sex differences in the effect of prepubertal GALP infusion on growth, metabolism and LH secretion. PHYSIOLOGY&BEHAVIOR 2007; 92(-5-):814-823. >>> Galanin-like peptide; Saline; CSF/CNS; Rat; 14 days; Controls received mp w/ vehicle; good methods pg. 815; ALZET brain infusion kit used; peptides; animal info (prepubescent, male, female, Long Evans, 45-60g.); 26-gauge cannula used.

P8859 Koo,J.W., Duman,R.S. IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-2-):751-756. >>> Interleukin-1 receptor antagonist; BSA; PBS; CSF/CNS; Rat; 2004; 21 days; Controls received mp w/ vehicle; immunology; ALZET brain infusion kit 2 used; animal info (adult, male, Sprague Dawley, 230-250g.).

P8858 Jin,D.Q., Sung,J.Y., Hwang,Y.K., Kwon,K.J., Han,S.H., Min,S.S., Han,J.S. Dexibuprofen (S+-isomer ibuprofen) reduces microglial activation and impairments of spatial working memory induced by chronic lipopolysaccharide infusion. Pharmacology Biochemistry and Behavior 2008; 89(-3-):404-411. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 28 days; Animal info (male, Wistar, 300-350g.); neurodegenerative (Alzheimer's disease); "Chronic infusions of LPS into rat brains produces many of the inflammatory symptoms, pathological changes, and memory impairments associated w/ AD".

P8853 Hook,V.Y.H., Kindy,M., Hook,G. Inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant beta-secretase site of the amyloid precursor protein. Journal of Biological Chemistry 2008; 283(-12-):7745-7753. >>> CAO74Me; E64d; Saline; Me2SO; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; no stress (see pg. 7747); enzyme inhibitor (cysteine protease); ALZET brain infusion kit 3 used; peptides; animal info (male, London APP, Swedish/London APP; 6, 12 months old); neurodegenerative (Alzheimer's disease); "Animals remained healthy and were observed to have normal eating, body weight, grooming + general appearance during the inhibitor treatment period. Of the 96 animals used in this study, none showed postoperative complications, such as infections; also, none died." (p. 7747).

P8850 Nakayama,H., Kitaichi,K., Ito,Y., Hashimotoa,K., Takagi,K., Yokoi,T., Takagi,K., Ozaki,N., Yamamoto,T., Hasegawa,T. The role of organic cation transporter-3 in methamphetamine disposition and its behavioral response in rats. Brain Research 2007; 1184(-;-):260-269. >>> Oligodeoxynucleotide, phosphorothioate antisense; oligodeoxynucleotide, phosphorothioate missense; Ringer's solution; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ missense ODN; no stress (see pg. 266); antisense (OCT3); ALZET brain infusion kit used; animal info (male, Wistar, 280-300g.); "The dose and infusion period of OCT3-AS and OCT-SCR did not produce any abnormal behavior or weight loss." (p. 266).

P8839 Augustine,R.A., Grattan,D.R. Induction of central leptin resistance in hyperphagic pseudopregnant rats by chronic prolactin infusion. Endocrinology 2008; 149(-3-):1049-1055. >>> Prolactin, ovine; CSF, artificial; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (female, Sprague Dawley, 10 wks old, 200-300g., pseudopregnant); endocrinology.

P8834 Festuccia,W.T., Oztezcan,S., Laplante,M., Berthiaume,M., Michel,C., Dohgu,S., Denis,R.G., Brito,M.N., Brito,N.A., Miller,D.S., Banks,W.A., Bartness,T.J., Richard,D., Deshaies,Y. Peroxisome proliferator-activated receptor-gamma-mediated positive energy balance in the rat is associated with reduced sympathetic drive to adipose tissues and thyroid status. Endocrinology 2008; 149(-5-):2121-2130. >>> Rosiglitazone maleate; Saline, sterile; DMSO; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; dose-response (Table 3); comparison of oral vs. mp; post op. care (ketoprophene); animal info (male, Sprague Dawley, 175-200g.); also known as BRL49653; 12.5% DMSO used; cannula position confirmed post mortem; endocrinology; PPAR-gamma agonist.

P8829 Zaheer,A., Zaheer,S., Thangavel,R., Wu,Y.H., Sahu,S.K., Yang,B.L. Glia maturation factor modulates beta-amyloid-induced glial activation, inflammatory cytokine/chemokine production and neuronal damage. Brain Research 2008; 1208(-;-):192-203. >>> Amyloid protein, beta (1-42), human; Saline; CSF/CNS; Mice; 2004; 28 days; Controls received mp w/ vehicle; peptides; animal info (C57BL/6, wt, GMF-KO, 5-6 months old); neurodegenerative (Alzheimer's disease).

P8809 Naef,L., Woodside,B. Prolactin/Leptin interactions in the control of food intake in rats. Endocrinology 2007; 148(-12-):5977-5983. >>> Prolactin, ovine; NaHCO3; HCl; CSF/CNS; Rat; 2001; 2002; 7, 10 days; Controls received mp w/ vehicle; dose-response (Fig. 1); peptides; animal info (female, Wistar, 220-240g.).

P8807 Sato,S., Hanada,R., Kimura,A., Abe,T., Matsumoto,T., Iwasaki,M., Inose,H., Ida,T., Mieda,M., Takeuchi,Y., Fukumoto,S., Fujita,T., Kato,S., Kangawa,K., Kojima,M., Shinomiya,K., Takeda,S. Central control of bone remodeling by neuromedin U. Nature Medicine 2007; 13(-10-):1234-1240. >>> Neuromedin U-23, rat; leptin, human; CSF/CNS; Mice; 28 days; Controls received mp w/ PBS; peptides; animal info (C57BL/6J, wt, LepOb, Nmu -/-, Adrb2 -/-, male, female, 3 months old).

P8806 Kalange,A.S., Kokare,D.M., Singru,P.S., Upadhya,M.A., Chopde,C.T., Subhedar,N.K. Central administration of selective melanocortin 4 receptor antagonist HS014 prevents morphine tolerance and withdrawal hyperalgesia. Brain Research 2007; 1181(--):10-20. >>> Morphine; CSF, artificial; NaOH; water, distilled; CSF/CNS; Rat; 2001; 7, 8 days; Controls received mp w/ vehicle; tolerance; dependence; animal info (male, Sprague Dawley, 220-260g.); cannula placement confimed with India ink.

P8803 Pinnock,S.B., Herbert,J. Brain-derived neurotropic factor and neurogenesis in the adult rat dentate gyrus: interactions with corticosterone. European Journal of Neuroscience 2008; 27(-10-):2493-2500. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, rat serum; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 2496) 1 animal died (cause unknown); peptides; animal info (male, Lister-Hooded, 200-250g.); cannula position confirmed post mortem with cresyl violet.

P8800 Park,S., Hong,S.M., Sung,S.R., Jung,H.K. Long-term effects of central leptin and resistin on body weight, insulin resistance, and beta-cell function and mass by the modulation of hypothalamic leptin and insulin signaling. Endocrinology 2008; 149(-2-):445-454. >>> Leptin, recomb. rat; resistin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; replacement therapy (pancreatectomy, 90%); peptides; animal info (male, Sprague Dawley, 220g).

P8797 Lee,M., Kim,A., Conwell,I.M., Hruby,V., Mayorou,A., Cai,M.M., Wardlaw,S.L. Effects of selective modulation of the central melanocortin-3-receptor on food intake and hypothalamic POMC expression. Peptides 2008; 29(-3-):440-447. >>> PG-932; [D-Trp8]-gamma-MSH; Saline; CSF/CNS; Rat; 2001; 1003D; 3, 7 days; 84, 66 hours; Controls received mp w/ vehicle; dose-response (fig 4, pg 445); pumps replaced after 7 days; peptides; animal info (male, Sprague-Dawley, 250-275 grams); agents are melanocortin-3-receptor agonists and antagonists ([D-Trp8]-gamma-MSH and PG-932 respectively).

P8794 Louis,S.A., Rietze,R.L., Deleyrolle,L., Wagey,R.E., Thomas,T.E., Eaves,A.C., Reynolds,B.A. Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay. Stem Cells 2008; 26(-4-):988-996. >>> Epidermal growth factor; ara-c; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; cyanoacrylate adhesive; animal info (adult, C57BL/6).

P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.

P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).

P8760 Yao,F.R., Sumners,C., O'Rourke,S.T., Sun,C.W. Angiotensin II increases GABAB receptor expression in nucleus tractus solitarii of rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-6-):H2712-H2720. >>> Angiotensin II; CSF/CNS; Rat; 1002; 2 weeks; Controls received mp w/ physiological saline; cardiovascular; peptides; animal info (male, Sprague Dawley 270-320g).

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P8751 Ghia,J.E., Blennerhassett,P., Collins,S.M. Impaired parasympathetic function increases susceptibility to inflammatory bowel disease in a mouse model of depression. Journal of Clinical Investigation 2008; 118(-6-):2209-2218. >>> Reserpine; Acetic acid; CSF/CNS; Mice; 14 days; Controls received mp w/ vehicle; dose-response (Fig. 1); comparison of IP injection vs. mp; no stress (see pg. 2210); antihypertensive; cyanoacrylate adhesive; animal info (male, female, C57BL/6, op/op, wt, 7-9 wks old, colitis, vagotomy); animal model of depression; plastics one cannula used; PE-60 used.

P8746 Ma,Q.L., Harris-White,M.E., Ubeda,O.J., Simmons,M., Beech,W., Lim,G.P., Teter,B., Frautschy,S.A., Cole,G.M. Evidence of A beta and transgene-dependent defects in ERK-CREB signaling in Alzheimer's models. Journal of Neurochemistry 2007; 103(-4-):1594-1607. >>> Antibody, monoclonal anti-amyloid peptide B (1-15); immunoglobulin IgG2b; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ non-specific mouse IgG2b; animal info (Tg2576, Tg-; 12, 22 months old); neurodegenerative (Alzheimer's disease); "AB vaccine or anti-AB antibodies are still among the most promising treatments for AD." (p. 1605).

P8743 Nogueiras,R., Wiedmer,P., Perez-Tilve,D., Veyrat-Durebex,C., Keogh,J.M., Sutton,G.M., Pfluger,P.T., Castaneda,T.R., Neschen,S., Hofmann,S.M., Howles,P.N., Morgan,D.A., Benoit,S.C., Szanto,I., Schrott,B., Schuermann,A., Joost,H.G., Hammond,C., Hui,D.Y., Woods,S.C., Rahmouni,K., Butler,A.A., Farooqi,I.S., O'Rahilly,S., Rohner-Jeanrenaud,F., Tschoep,M.H. The central melanocortin system directly controls peripheral lipid metabolism. Journal of Clinical Investigation 2007; 117(-11-):3475-3488. >>> SHU-9119; MTII; SC; CSF/CNS; Rat; 2001; 2, 7 days; Controls received mp w/ isotonic saline; no stress (see pg. 3476); ALZET brain infusion kit 3 used; post op. care (Buprenorphine); animal info (male, Wistar, 8-10 weeks old, 300-400g).

P8684 Fuezesi,T., Wittmann,G., Liposits,Z., Lechan,R.M., Fekete,C. Contribution of noradrenergic and adrenergic cell groups of the Brainstem and agouti-related protein-synthesizing neurons of the arcuate nucleus to neuropeptide-y innervation of corticotropin-releasing hormone neurons in hypothalamic Paraventricular nucleus of the rat. Endocrinology 2007; 148(-11-):5442-5450. >>> Neuropeptide Y; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; peptides; post op. care (bacitracin ointment over skull); animal info (Sprague-Dawley, male, 280-320g); 22-gauge stainless steel guide cannula, Plastics One.

P8683 Garcia-San Frutos,M., Fernandez-Agullo,T., De Solis,A.J., Andres,A., Arribas,C., Carrascosa,J.M., Ros,M. Impaired central insulin response in aged Wistar rats: Role of adiposity. Endocrinology 2007; 148(-11-):5238-5247. >>> Insulin, human; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; dose-response (fig. 1, p. 5241); peptides; animal info (Wistar, 3, 8, 24 month, male); endocrinology.

P8681 Grattan,D.R., Jasoni,C.L., Liu,X.H., Anderson,G.M., Herbison,A.E. Prolactin regulation of gonadotropin-releasing hormone neurons to suppress luteinizing hormone secretion in mice. Endocrinology 2007; 148(-9-):4344-4351. >>> Prolactin, ovine; CSF/CNS; Mice; 1002; 7 days; Serum levels taken, peptides, animal info (adult, female, C57BL6/J, 6-8 weeks, 24-28g, OVX).

P8678 Gallardo,N., Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Gomez-Alonso,S., Blanco,P., Carrascosa,J.M., Ros,M., Andres,A. Tissue-specific effects of central leptin on the expression of genes involved in lipid metabolism in liver and white adipose tissue. Endocrinology 2007; 148(-12-):5604-5610. >>> Leptin; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; peptides; animal info (3 months old, Wistar, male); endocrinology.

P8675 Olsson,M., Herrington,M.K., Reidelberger,R.D., Permert,J., Arnelo,U. Comparison of the effects of chronic central administration and chronic peripheral administration of islet amyloid polypeptide on food intake and meal pattern in the rat. Peptides 2007; 28(-7-):1416-1423. >>> Amylin; NaCl; SC; CSF/CNS; CSF/CNS (third ventricle); 2001; 7 days; Dose-response (p. 1418); comparison of ICV infusion vs. mp; peptides; animal info (male, Sprague-Dawley, 310-370g); "Doses in central administration experiments were 10-fold lower than those in the peripheral experiment..." (p. 1417); obesity.

P8672 Popp,E., Schneider,A., Vogel,P., Teschendorf,P., Boettiger,B.W. Time course of the hypothermic response to continuously administered neurotensin. Neuropeptides 2007; 41(-5-):349-354. >>> Neurotensin; Saline; CSF/CNS; Rat; 1003D; ; Controls received mp w/ vehicle; dose-response (pg 351, fig. 1); comparison of SC injections vs. mp; ALZET brain infusion kit 2 used; tolerance; peptides; animal info (Wistar-Han, male, 300-350g); "Diffusion of neurotensin into the pump was avoided by a drop of sesame oil" p. 350; "Complete surgery took about 20 min" p. 350; neurotensin is a tridecapeptide.

P8665 Reading,S.A., Brayden,J.E. Central role of TRPM4 channels in cerebral blood flow regulation. Stroke 2007; 38(-8-):2322-2328. >>> Oligonucleotide, phosphorothioate sense; oligonucleotide, phosphorothioate antisense; CSF, artificial; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ ODN, sense; antisense (TRPM4); ALZET braininfusion kit 1 used; animal info (male, Sprague-Dawley, 400-550g; "Efficient uptake of oligonucleotides by vascular myocytes in vivo may have resulted from the long exposure time during which the osmotic pumps continuously refresh the ODNs in the CSF over many days" (p. 2326).

P8660 Soliz,J., Gassmann,M., Joseph,V. Soluble erythropoietin receptor is present in the mouse brain and is required for the ventilatory acclimatization to hypoxia. JOURNAL OF PHYSIOLOGY-LONDON 2007; 583(-1-):329-336. >>> Erythropoietin receptor, soluble; Phosphate buffer; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male C57/BL6, 3 months old, hypoxia).

P8658 Rai,K.S., Hattiangady,B., Shetty,A.K. Enhanced production and dendritic growth of new dentate granule cells in the middle-aged hippocampus following intracerebroventricular FGF-2 infusions. European Journal of Neuroscience 2007; 26(-7-):1765-1779. >>> Fibroblast growth factor-2; CSF, artificial; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; good methods (p. 1766-1767); ALZET brain infusion kit used; cyanoacrylate adhesive; post op. care (tetracycline); animal info (male, F344, 12 months).

P8655 Gemma,C., Bachstetter,A.D., Cole,M.J., Fister,M., Hudson,C., Bickford,P.C. Blockade of caspase-1 increases neurogenesis in the aged hippocampus. European Journal of Neuroscience 2007; 26(-10-):2795-2803. >>> YVAD-CMK, Ac-; DMSO; saline; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; functionality of mp verified by mp weight (not suggested by manufacturer); enzyme inhibitor (caspase-1); animal info (male, Fischer 344; 4 and 22 months old); 0.6% DMSO.

P8647 Tohyama,Y., Mueck-Seler,D., Diksic,M. Acute fiesinoxan treatment produces a different effect on rat brain serotonin synthesis than chronic treatment: An alpha-methyl-L-tryptophan autoradiographic study. NEUROCHEMISTRY INTERNATIONAL 2007; 51(-8-):486-495. >>> Flesinoxan hydrochloride; Saline; SC; CSF/CNS; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; animal info (male, Sprague-Dawley, 170-210g).

P8646 Kuwata,K., Nishida,N., Matsumoto,T., Kamatari,Y.O., Hosokawa-Muto,J., Kodama,K., Nakamura,H.K., Kimura,K., Kawasaki,M., Takakura,Y., Shirabe,S., Takata,J., Kataoka,Y., Katamine,S. Hot spots in prion protein for pathogenic conversion. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-29-):11921-11926. >>> GN8; Glucose; saline; SC; CSF/CNS; Mice; ; Controls received mp w/ vehicle; animal info (4 wk old).

P8645 Adachi,K., Mirzadeh,Z., Sakaguchi,M., Yamashita,T., Nikolcheva,T., Gotoh,Y., Peltz,G., Gong,L., Kawase,T., varez-Buylla,A., Okano,H., Sawamoto,K. beta-Catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone. Stem Cells 2007; 25(-11-):2827-2836. >>> Ro3303544; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; enzyme inhibitor (protein kinase); animal info (adult, male, C57BL/6, 9 wks); GSK3-beta is a protein kinase.

P8644 Hamill,C.E., Caudle,W.M., Richardson,J.R., Yuan,H., Pennell,K.D., Greene,J.G., Miller,G.W., Traynelis,S.F. Exacerbation of Dopaminergic terminal damage in a mouse model of Parkinson's disease by the g-protein-coupled receptor protease-activated receptor 1. MOLECULAR PHARMACOLOGY 2007; 72(-3-):653-664. >>> BMS-200261; Hepes, buffered saline; CSF/CNS; Mice; 1003D; 2 days; Controls received mp w/ vehicle; animal info (male, C57BL/6, PAR1 +/-); neurodegenerative (Parkinson's Disease); BMS-200261 is a PAR1 antagonist (protease activated receptor); "The pumps were stored overnight at 37oC in sterile saline (0.9% NaCl) to prime drug release." (p. 654); "Placement was verified in a subset of animals by injection of 0.5 ul of Evans blue." (p. 654).

P8633 Chan,S.H.H., Wang,L.L., Tseng,H.L., Chan,J.Y.H. Upregulation of AT1 receptor gene on activation of protein kinase C beta/nicotinamide adenine dinucleotide diphosphate oxidase/ERK1/2/c-fos signaling cascade mediates long-term pressor effect of angiotensin II in rostral ventrolateral medulla. Journal of Hypertension 2007; 25(-9-):1845-1861. >>> Angiotensin II; CSF, artificial; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; comparison of ICV microinjections vs. mp; cardiovascular; peptides; post op. care (procaine penicillin); animal info (male, Sprague-Dawley, 200-280g).

P8632 Han,G.P., Li,L., Kosugi,I., Kawasaki,H., Tsuchida,T., Miura,K., Tsutsui,Y. Enhancement of susceptibility of adult mouse brain to cytomegalovirus infection by infusion of epidermal growth factor. Journal of Neuroscience Research 2007; 85(-13-):2981-2990. >>> Epidermal growth factor, mouse; PBS; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; peptides; animal info (female, BALB/c; 10, 25, and 70 wks old).

P8630 Lee,C.L., Kuo,T.F., Wang,J.J., Pan,T.M. Red mold rice ameliorates impairment of memory and learning ability in intracerebroventricular amyloid beta-infused rat by repressing amyloid beta accumulation. Journal of Neuroscience Research 2007; 85(-14-):3171-3182. >>> Amyloid protein, beta (1-40); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, Wistar, 280-320 g); neurodegenerative (Alzheimer's disease).

P8628 Basselin,M., Villacreses,N.E., Lee,H.J., Bell,J.M., Rapoport,S.I. Chronic lithium administration attenuates up-regulated brain arachidonic acid metabolism in a rat model of neuroinflammation. Journal of Neurochemistry 2007; 102(-3-):761-772. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 6 days; Controls received mp w/ vehicle; no stress (see pg. 763); animal info (male, Fischer, CDF (f-344)/Crl BR, 2 months old).

P8627 Huang,B.S., Ahmad,M., Tan,J., Leenen,F.H.H. Sympathetic hyperactivity and cardiac dysfunction post-MI: Different impact of specific CNS versus general AT1 receptor blockade. Journal of Molecular and Cellular Cardiology 2007; 43(-4-):479-486. >>> Losartan; spironolactone; CSF, artificial; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; comparison of SC injections vs. mp; no stress (see pg. 483); cardiovascular; ischemia (cardiac); animal info (male, wistar, 200-250g, coronary artery ligation MI).

P8626 Cho,S.R., Benraiss,A., Chmielnicki,E., Samdani,A., Economides,A., Goldman,S.A. Induction of neostriatal neurogenesis slows disease progression in a transgenic murine model of Huntington disease. Journal of Clinical Investigation 2007; 117(-10-):2889-2902. >>> Ara C; Saline; CSF/CNS; Mice (transgenic); 1002; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; ALZET brain infusion kit 3 used; animal info (5-9 weeks old); neurodegenerative (Huntington's disease).

P8586 Scarpace,P.J., Matheny,M., Zhang,Y., Cheng,K.Y., Tumer,N. Leptin antagonist reveals an uncoupling between leptin receptor signal transducer and activator of transcription 3 signaling and metabolic responses with central leptin resistance. J Pharmacol.Exp.Ther. 2007/2; 320(-2-):706-712. >>> Leptin antagonist; NaHCO3; CSF/CNS; 14 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; peptides; animal info (male, F344xBN, 3 months old, 275-375 grams).

P8560 Plum,L., Rother,E., Muenzberg,H., Wunderlich,F.T., Morgan,D.A., Hampel,B., Shanabrough,M., Janoschek,R., Koenner,A.C., Alber,J., Suzuki,A., Krone,W., Horvath,T.L., Rahmouni,K., Bruening,J.C. Enhanced leptin-stimulated Pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metabolism 2007; 6(-6-):431-445. >>> Leptin; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Peptides; animal info (male).

P8541 Muramatsu,Y., Furuichi,Y., Tojo,N., Moriguchi,A., Maemoto,T., Nakada,H., Hino,M., Matsuoka,N. Neuroprotective efficacy of FR901459, a novel derivative of cyclosporin A, in in vitro mitochondrial damage and in vivo transient cerebral ischemia models. Brain Research 2007; 1149(--):181-190. >>> Cyclosporin A; FR901459; Cremophor EL; saline;; CSF/CNS; Rat; 1003D; 48 hours; Dose-response (fig. 7); comparison of IV bolus injections vs. mp; ischemia (cerebral); animal info (male, Wistar, 9-10 weeks old, 274-280 grams, MCAO).

P8532 Moenkkoenen,K.S., Hakumaeki,J.M., Hirst,R.A., Miettinen,R.A., O'Callaghan,C., Maennistoe,P.T., Laitinen,J.T. Intracerebroventricular antisense knockdown of G alpha i2 results in ciliary stasis and ventricular dilatation in the rat. BMC NEUROSCIENCE 2007; 8(--):U12-U26. >>> Oligodeoxynucleotide, antisense; oligodeoxynucleotide, missense; Saline, sterile; CSF/CNS; Rat; 1007D; 2002; 7,11 days; Controls received mp w/ vehicle or nonsense or mismatched oligos; no stress (see pg. 2); antisense (G alpha i2); brain tissue distribution; animal info (male, Harlan Wistar, 3 weeks old, 50-100 grams, adult, 250 grams).

P8526 Randolph,C.L., Bierl,M.A., Isaacson,L.G. Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input. Brain Research 2007; 1144(--):59-69. >>> Nerve growth factor, mouse; Bisbenzimide; CSF/CNS; Rat; 2002; 2 weeks; Controls received no treatment; peptides; animal info (female, Sprague-Dawley, 3 months old); bisbenzimide (fluorescent marker) added to infusate to monitor cannula placement.

P8515 Ishii,T., Muranaka,R., Tashiro,O., Nishimura,M. Chronic intracerebroventricular administration of anti-neuropeptide Y antibody stimulates starvation-induced feeding via compensatory responses in the hypothalamus. Brain Research 2007; 1144(--):91-100. >>> Antibody, rabbit, anti-neuropeptide Y; Saline; CSF/CNS; Mice; 2002; 13 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; ALZET brain infusion kit 3 used; animal info (male, ddy, 7 weeks old, 35-40 g).

P8513 Gomori,A., Ishihara,A., Ito,M., Matsushita,H., Ito,M., Mashiko,S., Iwaasa,H., Matsuda,M., Bednarek,M.A., Qian,S., MacNeil,D.J., Kanatani,A. Blockade of MCH1 receptor signalling ameliorates obesity and related hepatic steatosis in ovariectomized mice. Br.J.Pharmacol. 2007/7; 151(-6-):900-908. >>> Melanin-concentrating hormone 1, receptor antagonist; Propylene glycol; water, distilled; CSF/CNS; Mice; 2004; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks of vehicle delivery; ALZET brain infusion kit used; post op. care (cefamezin-a); animal info (female, C57BL/6J, 12 weeks old, female Mch1r KD, 14-16 weeks old, 22 g); cannula placement confirmed with Evans Blue dye; obesity.

P8507 Stranahan,A.M., Arumugam,T.V., Cutler,R.G., Lee,K., Egan,J.M., Mattson,M.P. Diabetes impairs hippocampal function through glucocorticoid-mediated effects on new and mature neurons. Nat.Neurosci. 2008/3; 11(-3-):309-317. >>> Ara-C; CSF/CNS; Mice; 1002; ; Diabetes; animal info (2 months old, wild-type); Ara-C is an anti-mitotic drug.

P8500 Xue,B.J., Pamidimukkala,J., Lubahn,D.B., Hay,M. Estrogen receptor-alpha mediates estrogen protection from angiotensin II- induced hypertension in conscious female. American Journal of Physiology-Heart and Circulatory Physiology 2007; 292(-4-):H1770-H1776. >>> Angiotensin II; Estradiol, 17B-; ICI-182,780; CSF/CNS; SC; Mice; 1002; ; Blood pressure measured; cardiovascular; ALZET brain infusion kit used; peptides; animal info (OVX, WT, and ER knockout); CNS group delivered E2 + ICI.

P8497 Strbian,D., Karjalainen-Lindsberg,M.L., Kovanen,P.T., Tatlisumak,T., Lindsberg,P.J. Mast cell stabilization reduces hemorrhage formation and mortality after administration of thrombolytics in experimental ischemic stroke. Circulation 2007; 116(-4-):411-418. >>> Cromoglycate; CSF/CNS; Rat; 2001; ; ALZET brain infusion kit 2 used; ischemia (cerebral); animal info (13-14 weeks old).

P8488 Smith,J.M., Lunga,P., Story,D., Harris,N., Le Belle,J., James,M.F., Pickard,J.D., Fawcett,J.W. Inosine promotes recovery of skilled motor function in a model of focal brain injury. Brain 2007; 130(--):915-925. >>> Inosine; CSF, artificial; CSF/CNS; Rat; 2004; 4 weeks; ALZET Brain Infusion Kit 2 used; multiple pumps per animal (2); post op. care (carprofen, IP); traumatic brain inujury; behavioral study; dental cement used; animal info (male, lister, hooded, 200-300 g).

P8431 Pan,Y.X., Gao,L., Wang,W.Z., Zheng,H., Liu,D.M., Patel,K.P., Zucker,I.H., Wang,W. Exercise training prevents arterial baroreflex dysfunction in rats treated with central angiotensin II. Hypertension 2007; 49(-3-):519-527. >>> Angiotensin II; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ aCSF; cardiovascular; ALZET brain infusion kit II used; peptides; animal info (male, Sprague-Dawley, 300-310g.); cannula placement confirmed with chicago blue dye.

P8428 Iwashita,A., Muramatsu,Y., Yamazaki,T., Muramoto,M., Kita,Y., Yamazaki,S., Mihara,K., Moriguchi,A., Matsuoka,N. Neuroprotective efficacy of the peroxisome proliferator-activated receptor delta-selective Agonists in vitro and in vivo. Journal of Pharmacology and Experimental Therapeutics 2007; 320(-3-):1087-1096. >>> L-165041; GW501516; PEG 300; saline; DMSO; CSF/CNS; Rat; mice; 1003D; 1007D; 2, 6 days; Controls received mp w/ vehicle; dose-response (fig. 6); cardiovascular; ischemia (cerebral); animal info (male, Wistar, 9-10 wks old, 250-280 g.; male, C57/BL6, 9-10 wks old, 19-22g.); neurodegenerative (Parkinson's disease); Right middle cerebral artery occlusion; 30% DMSO.

P8422 Lavoie,N., Peralta,MR III, Chiasson,M., Lafortune,K., Pellegrini,L., Seress,L., Toth,K. Extracellular chelation of zinc does not affect hippocampal excitability and seizure-induced cell death in rats. JOURNAL OF PHYSIOLOGY-LONDON 2007; 578(-1-):275-289. >>> Tricine; EDTA, copper; EDTA, zinc; EDPA; EDTA, calcium; Saline; cresyl violet; CSF/CNS; Rat; 2001D; 12 hours; Controls received mp w/ vehicle; functionality of mp verified by residual volume; brain tissue distribution; animal info (male, Sprague-Dawley, >35 days old); cresyl violet used to verify injection site.

P8420 Jang,S., Lee,J.H., Choi,K.R., Kim,D., Yoo,H.S., Oh,S. Cytochemical alterations in the rat retina by LPS administration. Neurochemical Research 2007; 32(-1-):1-10. >>> Endotoxin, LPS; sphingosine-1-phosphate; CSF/CNS; Rat; 2ML1; 7 days; Controls received mp w/ saline; cyanoacrylate adhesive; animal info (male, Sprague-Dawley, 260-280g).

P8413 Ochedalski,T., Subburaju,S., Wynn,P.C., Aguilera,G. Interaction between oestrogen and oxytocin on hypothalamic-pituitary-adrenal axis activity. Journal of Neuroendocrinology 2007; 19(-3-):189-197. >>> Oxytocin; Saline, sterile; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); ALZET brain infusion kit used; animal info (female, Sprague-Dawley, 250-300g.).

P8409 Prada,C.M., Garcia-Alloza,M., Betensky,R.A., Zhang-Nunes,S.X., Greenberg,S.M., Bacskai,B.J., Frosch,M.P. Antibody-mediated clearance of amyloid-beta peptide from cerebral amyloid angiopathy revealed by quantitative in vivo imaging. Journal of Neuroscience 2007; 27(-8-):1973-1980. >>> Antibody, anti-amyloid protein beta (10D5); antibody, anti-human tau (16B5); Dye, indocyanine green; CSF/CNS; Mice (transgenic); 2002; 14 days; Controls received mp w/ anti-Tau AB; comparison of single CNS injection vs. mp; animal info (Tg2576, 10-13 months old); neurodegenerative (Alzheimer's disease, CAA); permanent cranial window for serial imaging with multiphoton laser-scanning microscopy; "with continous infusion of anti-AB antibody, the clearance of CAA was more robust and continued throughout the duration of the treatment." (pg. 1978).

P8406 Zeevalk,G.D., Manzino,L., Sonsalla,P.K., Bernard,L.P. Characterization of intracellular elevation of glutathione (GSH) with glutathione monoethyl ester and GSH in brain and neuronal cultures: Relevance to Parkinson's disease. Experimental Neurology 2007; 203(-2-):512-520. >>> Glutathione, monoethyl ester; MPP+; Saline; SC; CSF/CNS; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; dose-response (fig. 6); stability verified by incubation for 0-28 days at 37 celsius, assessed by HPLC; half-life (pg. 515) 10-14 hours in culture; brain tissue distribution; toxicology; animal info (Sprague-Dawley, 300g.); neurodegenerative (Parkinson's disease).

P8403 Marchalant,Y., Rosi,S., Wenk,G.L. Anti-inflammatory property of the cannabinoid agonist WIN-55212-2 in a rodent model of chronic brain inflammation. Neuroscience 2007; 144(-4-):1516-1522. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 21 days; Controls received mp w/ vehicle; no stress (see pg. 1518); animal info (male, F-344, 3 months old); neurodegenerative (Alzheimer's disease, Parkinson's disease, MS); Neuroinflammation; "Chronic infusion of LPS and WIN-55212-2 injections was well tolerated by all rats: they gained weight normally for the duration of the study." (pg. 1518); plastics one cannula used.

P8386 Serralta,A., Barcia,J.A., Ortiz,P., Duran,C., Hernandez,M.E., Alos,M. Effect of intracerebroventricular continuous infusion of valproic acid versus single i.p. and i.c.v. injections in the amygdala kindling epilepsy model. Epilepsy Research 2006; 70(-1-):15-26. >>> Valproic acid; Saline; CSF/CNS; Rat; 2001; 2002; 7 days; Controls received no treatment; dose-response (fig. 1); comparison of icv, IP injections vs. mp; no stress (see pg. 22); toxicology; multiple pumps per animal (2); animal info (male, Wistar, amygdala-kindled epilepsy, 380-420g.); mp primed 1 hr. in 37.5 celsius saline; evans blue dye used to confirm cannula placement, "chronic i.c.v. VPA infusion affords protection against kindled seizures accompanied by less ataxia and sedation" (pg. 24).

P8331 Joppa,M.A., Gogas,K.R., Foster,A.C., Markison,S. Central infusion of the melanocortin receptor antagonist agouti-related peptide (AgRP(83-132)) prevents cachexia-related symptoms induced by radiation and colon-26 tumors in mice. Peptides 2007; 28(-3-):636-642. >>> Agouti-related peptide (83-132); Saline, sterile; CSF/CNS; Mice; 1003D; 72 hours; Controls received mp w/ vehicle; cancer (colon adenocarcinoma); peptides; animal info (male, BALB/C, 6 weeks old, 22 grams); Research Diets D12689B.

P8327 Schwenke,D.O., Pearson,J.T., Mori,H., Shirai,M. Does central nitric oxide elicit pulmonary hypertension in conscious rats?. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2006; 153(-3-):250-260. >>> L-NAME; SIN-1; CSF, artificial; CSF/CNS; Rat; 2004; 3 weeks; Controls received mp w/ vehicle; enzyme inhibitor (NO synthase); cardiovascular; animal info (male, Sprague-Dawley, 200-320 grams, 8 weeks old); Evans blue dye used to confirm correct cannula placement; SIN-1 is 2-[4-morpholinyl]-sydnonimine-hydrochloride.

P8322 Sakaguchi,M., Shingo,T., Shimazaki,T., Okano,H.J., Shiwa,M., Ishibashi,S., Oguro,H., Ninomiya,M., Kadoya,T., Horie,H., Shibuya,A., Mizusawa,H., Poirier,F., Nakauchi,H., Sawamoto,K., Okano,H. A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-18-):7112-7117. >>> Antibody, anti-galectin-1; immunoglobulin G, rabbit; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle or rabbit IgG; peptides; animal info (male, 1295J wt or galectin-1 -/-, 8 weeks old).

P8319 Burgos-Ramos,E., Hervas-Aguilar,A., Puebla-Jimenez,L., Boyano-Adanez,M.C., rilla-Ferreiro,E. Chronic but not acute intracerebroventricular administration of amyloid beta-peptide(25-35) decreases somatostatin content, adenylate cyclase activity, somatostatin-induced inhibition of adenylate cyclase activity, and adenylate cyclase I levels in the rat hippocampus. Journal of Neuroscience Research 2007; 85(-2-):433-442. >>> Amyloid protein, beta (25-35); amyloid protein, beta (35-25); Acetic acid; CSF/CNS; Rat; 14 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp peptides; animal info (male, Wistar, 200-250 grams); neurodegenerative (Alzheimer's disease).

P8316 Ohta,S., Gregg,C., Weiss,S. Pituitary adenylate cyclase-activating polypeptide regulates forebrain neural stem cells and neurogenesis in vitro and in vivo. Journal of Neuroscience Research 2006; 84(-6-):1177-1186. >>> Pituitary adenylate cyclase-activating polypeptide; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; peptides; animal info (CD1, 6-7 wks old).

P8300 Messina,M.M., Overton,J.M. Cardiovascular effects of melanin-concentrating hormone. REGULATORY PEPTIDES 2007; 139(-1-3-):23-30. >>> Melanin-concentrating hormone; PBS; BSA; CSF/CNS; Rat; 1002; 10 days; Controls received mp w/ vehicle; dose-response (fig. 1); comparison of acute icv infusion vs. mp; peptides; post op. care (Bupivacaine); animal info (male, Long-Evans, 330g.).

P8291 Won,S.J., Xie,L., Kim,S.H., Tang,H.D., Wang,Y.M., Mao,X.O., Banwait,S., Jin,K.L. Influence of age on the response to fibroblast growth factor-2 treatment in a rat model of stroke. Brain Research 2006; 1123(--):237-244. >>> Fibroblast growth factor-2; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; animal info (24 month old/3 month old, male, Fisher); ischemia (cerebral).

P8284 Yazdani,U., German,D.C., Liang,C.L., Manzino,L., Sonsalla,P.K., Zeevalk,G.D. Rat model of Parkinson's disease: Chronic central delivery of 1-methyl-4-phenylpyridinium (MPP+). Experimental Neurology 2006; 200(-1-):172-183. >>> MPP+; Saline-iodine; CSF/CNS; Rat; 2ML4; 14 days; Stability verified by HPLC, 28 days; animal info (male, Sprague-Dawley, 300-350 grams); neurodegenerative (Parkinson's Disease); "This study presents a chronic model of PD in rats that encompasses the desirable components of current chronic models while reducing their less desirable aspects." P. 181.

P8283 Yau,J.L.W., Noble,J., Graham,M., Seckl,J.R. Central administration of a cytochrome P450- 7 alpha product 7 alpha-hydroxypregnenolone improves spatial memory retention in cognitively impaired aged rats. Journal of Neuroscience 2006; 26(-43-):11034-11040. >>> Pregnenolone; dehydroepiandrosteone; CSF, artificial; cyclodextrin, hydroxypropyl, beta; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male, Lister hooded, 29 months, 500-600 grams); dental cement used to secure cannula; behavioral study; 10% cyclodextrin used; aging.

P8276 Rustamzadeh,E., Hall,W.A., Todhunter,D.A., Vallera,V.D., Low,W.C., Liu,H.Y., Panoskaltsis-Mortari,A., Vallera,D.A. Intracranial therapy of glioblastoma with the fusion protein DTAT in immunodeficient mice. International Journal of Cancer 2006; 120(-2-):411-419. >>> DTAT; Saline; CSF/CNS; Mice; 1007D; ; Controls received mp w/ vehicle; dose-response (table 1); comparison of bolus ICV injections vs. mp; cancer (glioblastoma); toxicology; peptides; animal info (C57BL/6, female, 12 weeks old); diptheria toxin; "delivery of DTAT resulted in about a 16 fold increase in maximum tolerated dose." (p. 411); "The results of this experiment indicate that the toxicity of uPAR can be reduced by CED (mp) delivery." (p. 416).

P8267 Warner-Schmidt,J.L., Duman,R.S. VEGF is an essential mediator of the neurogenic and behavioral actions of antidepressants. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-11-):4647-4652. >>> Vascular endothelial growth Factor 164, recomb. rat; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; peptides; animal info (male, Sprague-Dawley, 175-200g.).

P8266 Urushitani,M., bou Ezzi,S., Julien,J.P. Therapeutic effects of immunization with mutant superoxide dismutase in mice models of amyotrophic lateral sclerosis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-7-):2495-2500. >>> Antibody, mouse anti hSOD1; antibody, rabbit polyclonal anit-hSOD1-FITC; Saline; CSF/CNS; Mice; 2004; 16, 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; stability verified by Western Blot w/ residual volume AB after 18 days infusion; brain tissue distribution; animal info (G93A SOD1, 85 days old); neurodegenerative (ALS).

P8263 Nishimura,M., Takahashi,H., Yoshimura,M. Upregulation of the brain renin-angiotensin system in rats with chronic renal failure. ACTA PHYSIOLOGICA 2007; 189(-4-):369-377. >>> Candesartan; CSF, artificial; IV (jugular); CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; replacement therapy (subtotal nephrectomy, dorsal rhizotomy); antihypertensive; cyanoacrylate adhesive; animal info (male, SHR/Izumo, 8 wks old, 300-350g.); ATI receptor antagonist.

P8262 Nogueiras,R., Tovar,S., Mitchell,S.E., Barrett,P., Rayner,D.V., Dieguez,C., Williams,L.M. Negative energy balance and leptin regulate neuromedin-U expression in the rat pars tuberalis. Journal of Endocrinology 2006; 190(-2-):545-553. >>> Leptin, recomb. human; Saline; CSF/CNS; Rat; 2ML2; 7 days; 24, 48 hours; Controls received mp w/ vehicle; dose-response (fig. 6); peptides; animal info (male, Sprague-Dawley, 8-10 wks old, 300g.); cannula placement confirmed by methylene blue staining; endocrinology.

P8258 Rosi,S., Vazdarjanova,A., Ramirez-Amaya,V., Worley,P.F., Barnes,C.A., Wenk,G.L. Memantine protects against LPS-induced neuroinflammation, restores behaviorally-induced gene expression and spatial learning in the rat. Neuroscience 2006; 142(-4-):1303-1315. >>> Endotoxin, LPS; memantine; CSF, artificial; SC; CSF/CNS (fourth ventricle); Rat; 2004; 2ML4; 28 days; Controls received mp w/ vehicle; no stress (see pg. 1308); multiple pumps per animal (2); animal info (male, F-344, 3 months old); neurodegenerative (Alzheimer's disease, Multiple Sclerosis, Amyotrophic Lateral Sclerosis, etc.).

P8253 Leker,R.R., Soldner,F., Velasco,I., Gavin,D.K., ndroutsellis-Theotokis,A., McKay,R.D.G. Long-lasting regeneration after ischemia in the cerebral cortex. Stroke 2007; 38(-1-):153-161. >>> Ara-C; Saline; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; ischemia (cerebral); animal info (SHR, permanent distal middle cerebral artery occlusion).

P8246 Sud,R., Ignatowski,T.A., Lo,C.P.K., Spengler,R.N. Uncovering molecular elements of brain-body communication during development and treatment of neuropathic pain. BRAIN BEHAVIOR AND IMMUNITY 2007; 21(-1-):112-124. >>> Tumor necrosis Factor-a, recomb. rat: tumor necrosis factor-a, recomb. rat, heat-inactivated; amitriptyline; CSF, artificial; CSF/CNS; Rat; 8 days; Controls received mp w/ vehicle, or heat-inactivated rr-TNFa; comparison of ip injections vs. mp; peptides; animal info (male, Sprague-Dawley, 300-350g); chronic constriction injury to the right sciatic nerve.

P8236 Lecanu,L., Greeson,J., Papadopoulos,V. Beta-amyloid and oxidative stress jointly induce neuronal death, amyloid deposits, gliosis, and memory impairment in the rat brain. Pharmacology 2006; 76(-1-):19-33. >>> Amyloid protein, beta 42; ferrous sulfate; buthionine sulfoximine; CSF, artificial; water, distilled; CSF/CNS; Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; stability verified by 14 days in mp at 37 C; animal info (male, Long-Evans, 3-4 month old, 300-325 grams); neurodegenerative (Alzheimer's disease).

P8233 D'Addario,C., Di Benedetto,M., Izenwasser,S., Candeletti,S., Romualdi,P. Role of serotonin in the regulation of the dynorphinergic system by a kappa-opoid agonist and cocaine treatment in rat CNS. Neuroscience 2007; 144(-1-):157-164. >>> Cocaine; Water, sterile; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 200-250 grams).

P8225 Kondo,N., Sekijima,T., Kondo,J., Takamatsu,N., Tohya,K., Ohtsu,T. Circannual control of hibernation by HP complex in the brain. Cell 2006; 125(-1-):161-172. >>> Antibody, polyclonal, anti-HP20c; immunoglobulin G, rabbit; PBS; CSF/CNS; Chipmunk; 2002; 2 weeks; Controls received mp w/ IgG; dose-response (fig. 6); animal info (male, tamias sibiricus, hibernating).

P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).

P8183 Hill-Pryor,C., Dunbar,J.C. The effect of high fat-induced obesity on cardiovascular and physical activity and opioid responsiveness in conscious rats. Clinical and Experimental Hypertension 2006; 28(-2-):133-145. >>> Funaltrexamine, B-; DAMGO; CSF/CNS; Rat; 2ML4; 2 weeks; Controls received mp w/ aCSF; cardiovascular; peptides; animal info (male, Wistar, 275-300 grams); mu opioid agonist, antagonist.

P8178 Robichaud,M., Debonnel,G. Allopregnanolone and ganaxolone increase the firing activity of dorsal raphe nucleus serotonergic neurons in female rats. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY 2006; 9(-2-):191-200. >>> Allopregnanolone; citalopram; RU-486; ganaxolone; Water, distilled; ethanol; SC; CSF/CNS; Rat; 3, 7 days; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 250-325 grams); 3% ethanol used.

P8166 Di Benedetto,M., D'Addario,C., Candeletti,S., Romualdi,P. Alterations of CREB and DARPP-32 phosphorylation following cocaine and monoaminergic uptake inhibitors. Brain Research 2007; 1128(-1-):33-39. >>> Cocaine; fluoxetine; GBR12909; nisoxetine; Water, sterile; SC; CSF/CNS; Rat; 2001; 2ML1; 7 days; Controls received mp w/ vehicle; no stress (see pg. 37); animal info (male, Sprague-Dawley, 200-250 grams).

P8153 Bauer,S., Patterson,P.H. Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. Journal of Neuroscience 2006; 26(-46-):12089-12099. >>> Ara-C; leukemia inhibitory factor, recomb. mouse; epidermal growth factor, recomb. human; Saline; PBS; BSA; CSF/CNS; Mice; 1003D; 1007D; 1, 3, 6, 7, days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 or 3 used; peptides; animal info (adult, male, C57BL/6J, 2-6 months old).

P8149 Kovacs,P., Morales,J.C., Karkanias,G.B. The effect of diabetes and centrally administered insulin on anterior hypothalamic estrogen receptor alpha immunoreactivity. ACTA DIABETOLOGICA 2007; 44(-1-):38-44. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (STZ-induced Diabetes, ovariectomy); peptides; animal info (female, Sprague-Dawley, 175-200g.); tissue perfusion (ventricle).

P8142 Iores-Marcal,L.M., Viel,T.A., Buck,H.S., Nunes,V.A., Gozzo,A.J., Cruz-Silva,I., Miranda,A., Shimamoto,K., Ura,N., Araujo,M.S. Bradykinin release and inactivation in brain of rats submitted to an experimental model of Alzheimer's disease. Peptides 2006; 27(-12-):3363-3369. >>> Amyloid protein, beta (1-40), human; E-64; HEPES; E-64; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; brain tissue distribution; peptides; animal info (male, Wistar, 3 mo. old); neurodegenerative (Alzheimer's disease); cathepsin B inhibitor.

P8141 Huang,Q.L., Timofeeva,E., Richard,D. Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. Journal of Endocrinology 2006; 191(-1-):179-188. >>> Leptin, recomb. murine; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ PBS; peptides; animal info (male, Wistar, 270-290g.); tissue perfusion (ventricle).

P8123 Lensman,M., Korzhevskii,D.E., Mourovets,V.O., Kostkin,V.B., Izvarina,N., Perasso,L., Gandolfo,C., Otellin,V.A., Polenov,S.A., Balestrino,M. Intracerebroventricular administration of creatine protects against damage by global cerebral ischemia in rat. Brain Research 2006; 1114(--):187-194. >>> Creatine; glycerol; Saline; CSF/CNS; Rat; 1002; 7, 13 days; Controls received mp w/ vehicle, or glycerol in saline; no stress (see pg.193); ischemia (cerebral); animal info (male, Sprague-Dawley, 270-320g, carotid artery occlusion); mp use overcomes the "poor brain bioavailability of systemically administered creatine. " (p.188).

P8119 Knauf,C., Rieusset,J., Foretz,M., Cani,P.D., Uldry,M., Hosokawa,M., Martinez,E., Bringart,M., Waget,A., Kersten,S., Desvergne,B., Gremlich,S., Wahli,W., Seydoux,J., Delzenne,N.M., Thorens,B., Burcelin,R. Peroxisome proliferator-activated receptor alpha null mice have increased white adipose tissue glucose utilization, GLUT4, and fat mass: Role in liver and brain. Endocrinology 2006; 147(-9-):4067-4078. >>> Wy-14643; DMSO; saline; CSF/CNS; Mice; 7 days; Controls received mp w/ vehicle; comparison of icv 3 hr. acute infusion vs. mp; no stress (see p.4072); Alzet brain infusion kit used; animal info (C57BL/6, wt or female PPAR -/-; 12-14 wks old, 20-25g.); 50% DMSO; endocrinology.

P8113 Kanehisa,M., Akiyoshi,J., Kitaichi,T., Matsushita,H., Tanaka,E., Kodama,K., Hanada,H., Isogawa,K. Administration of antisense DNA for ghrelin causes an antidepressant and anxiolytic response in rats. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY&BIOLOGICAL PSYCHIATRY 2006; 30(-8-):1403-1407. >>> Oligodeoxynucleotide, antisense; oligodeoxynucleotide, missense; Saline; CSF/CNS; Rat; 1003D; 1007D; 3 days; Controls received mp w/ vehicle, or missense oligo; antisense (ghrelin); post op. care (ceftriaxone sodium); animal info (male, Wistar, 7-9 wks old, 300-330g.).

P8111 Jackson,E.L., Garcia-Verdugo,J.M., Gil-Perotin,S., Roy,M., Quinones-Hinojosa,A., VandenBerg,S., varez-Buylla,A. PDGFR-alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 2006; 51(-2-):187-199. >>> Platelet-derived growth factor-AA; Saline; BSA; CSF/CNS; Mice; 6, 14 days; Controls received mp w/ vehicle; comparison of icv injections vs. mp; peptides; animal info (CD-1, GFAP-Tva, 2-4 months old).

P8106 Liu,D.M., Gao,L., Roy,S.K., Cornish,K.G., Zucker,I.H. Neuronal angiotensin II type 1 receptor upregulation in heart failure - Activation of activator protein 1 and jun N-terminal kinase. Circulation Research 2006; 99(-9-):1004-1011. >>> Angiotensin II; losartan; CSF, artificial; CSF/CNS; Rabbit; 2001; 6 days; Controls received no treatment; cardiovascular; peptides; animal info (male, New Zealand White, 3.2-4.1 kg., CHF).

P8085 Date,I., Takagi,N., Takagi,K., Tanonaka,K., Funakoshi,H., Matsumoto,K., Nakamura,T., Takeo,S. Hepatocyte growth factor attenuates cerebral ischemia-induced increase in permeability of the blood-brain barrier and decreases in expression of tight junctional proteins in cerebral vessels. Neuroscience Letters 2006; 407(-2-):141-145. >>> Hepatocyte growth factor, recomb. human; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ physiological saline; peptides; ischemia (cerebral); animal info (male, Wistar, 180-220g, microsphere-induced cerebral embolism).

P8064 Zhang,B., Hata,R., Zhu,P.X., Sato,K., Wen,T.C., Yang,L.H., Fujita,H., Mitsuda,N., Tanaka,J., Samukawa,K., Maeda,N., Sakanaka,M. Prevention of ischemic neuronal death by intravenous infusion of a ginseng saponin, ginsenoside Rb(1), that upregulates Bcl-x(L) expression. Journal of Cerebral Blood Flow and Metabolism 2006; 26(-5-):708-721. >>> Ginsenoside Rb1; Saline, isotonic; IV (femoral); CSF/CNS; Rat; gerbil; 2001; 2004; 4 weeks; 1, 7 days; Controls received mp w/ vehicle; dose-response (fig. 2); cardiovascular; ischemia (cerebral); animal info (SHR-SP, 16-18 wk old, 250-300g. MCAO; male, Mongolian Gerbil, 70-80g., 12 wk old, MCAO).

P8062 Gelling,R.W., Morton,G.J., Morrison,C.D., Niswender,K.D., Myers,MG Jr, Rhodes,C.J., Schwartz,M.W. Insulin action in the brain contributes to glucose lowering during insulin treatment of diabetes. Cell Metabolism 2006; 3(-1-):67-73. >>> LY294002; CSF/CNS (third ventricle); Rat; 2002; 9 days; Controls received mp w/ vehicle; comparison of icv injections vs. mp; enzyme inhibitor (phosphatidylinositol 3-kinase); animal info (male, Sprague-Dawley, 250-350g); cannula placement confirmed; tissue perfusion (ventricle).

P8054 Cifani,C., Guerrini,R., Massi,M., Polidori,C. Chronic intracerebroventricular infusion of nociceptin/orphanin FQ increases food and ethanol intake in alcohol-preferring rats. Peptides 2006; 27(-11-):2803-2810. >>> Nociceptin/orphanin FQ; Saline; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig.1); peptides; post op.care (benzyl penicillin, dihydrostreptomycin); animal info(Marchigian-Sardinian alcohol-preferring (msp), male, 250-300g.); cannula placement verified with india ink injection at experiment end.

P8045 Thompson,H.J., Marklund,N., LeBold,D.G., Morales,D.M., Keck,C.A., Vinson,M., Royo,N.C., Grundy,R., McIntosh,T.K. Tissue sparing and functional recovery following experimental traumatic brain injury is provided by treatment with an anti-myelin-associated glycoprotein antibody. European Journal of Neuroscience 2006; 24(-11-):3063-3072. >>> Antibody, anti-MAG mAb; CSF/CNS; 1003D; 3 days; ALZET brain infusion kit 1 used; brain tissue distribution; animal info (male, Sprague-Dawley, 350-400 grams); cannula secured via tissue adhesive; 35 mm long PE tubing used.

P8040 Zhang,J., Matheny,M.K., Tuemer,N., Mitchell,M.K., Scarpace,P.J. Leptin antagonist reveals that the normalization of caloric intake and the thermic effect of food after high-fat feeding are leptin dependent. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 292(-2-):R868-R874. >>> Leptin; leptin antagonist; Sodium bicarbonate; CSF/CNS; 2001; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; peptides; animal info (male, Fischer, Brown Norway); dental cement used to secure cannula.

P8035 Nogueiras,R., Pfluger,P., Tovar,S., Arnold,M., Mitchell,S., Morris,A., Perez-Tilve,D., Vazquez,M.J., Wiedmer,P., Castaneda,T.R., DiMarchi,R., Tschoep,M., Schurmann,A., Joost,H.G., Williams,L.M., Langhans,W., Dieguez,C. Effects of obestatin on energy balance and growth hormone secretion in rodents. Endocrinology 2007; 148(-1-):21-26. >>> Obestatin; Saline; CSF/CNS; Rat; 2ML2; 2001D; 7 days; Controls received mp w/ vehicle; animal info (adult, male, 10-12 weeks old, 350-360 grams); obesity.

P8023 Niewiadomska,G., Baksalerska-Pazera,M., Gasiorowska,A., Mietelska,A. Nerve growth factor differentially affects spatial and recognition memory in aged rats. Neurochemical Research 2006; 31(-12-):1481-1490. >>> Nerve growth factor, beta; CSF, artificial; CSF/CNS; Rat; 2004; 1 month; ALZET brain infusion kit used; post op. care (antibiotic and analgesic to scalp + fluid therapy); silastic tubing used; 28 G cannula.

P7987 Wang,Y.M., Galvan,V., Gorostiza,O., Ataie,M., Jin,K.L., Greenberg,D.A. Vascular endothelial growth factor improves recovery of sensorimotor and cognitive deficits after focal cerebral ischemia in the rat. Brain Research 2006; 1115(--):186-193. >>> Vascular endothelial growth factor; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); behavioral study.

P7973 Bartolomucci,A., La Corte,G., Possenti,R., Locatelli,V., Rigamonti,A.E., Torsello,A., Bresciani,E., Bulgarelli,I., Rizzi,R., Pavone,F., D'Amato,F.R., Severini,C., Mignogna,G., Giorgi,A., Schinina,M.E., Elia,G., Brancia,C., Ferri,G.L., Conti,R., Ciani,B., Pascucci,T., Dell'Omo,G., Muller,E.E., Levi,A., Moles,A. TLQP-21, a VGF-derived peptide, increases energy expenditure and prevents the early phase of diet-induced obesity. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-39-):14584-14589. >>> TLQP-21, synthetic; CSF, artificial; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ vehicle; stability verified by HPLC after 14 days at 37 C; ALZET brain infusion kit 1 used; peptides; animal info (male, CD1); obesity.

P7966 Oh,I., Shimizu,H., Satoh,T., Okada,S., Adachi,S., Inoue,K., Eguchi,H., Yamamoto,M., Imaki,T., Hashimoto,K., Tsuchiya,T., Monden,T., Horiguchi,K., Yamada,M., Mori,M. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443(-7112-):709-712. >>> NUCB2; CSF/CNS; Rat; 10 days; Animal info (male, Wistar, 200-250 grams).

P7932 Zeinoaldini,S., Swarts,H.J.M., Van de Heijning,B.J.M. Effects of central infusion and immunoneutralization of growth hormone on the timing of puberty and plasma leptin levels in the female rat. REGULATORY PEPTIDES 2006; 134(-2-3-):158-163. >>> Growth hormone, recomb. human; immunoglobulin G, goat; growth hormone, anti-rat; Saline, sterile; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides; post op. care (analgesia, ibuprofen); animal info (female, Wistar, 45 grams).

P7925 Tada,T., Zhan,H., Tanaka,Y., Hongo,K., Matsumoto,K., Nakamura,T. Intraventricular administration of hepatocyte growth factor treats mouse communicating hydrocephalus induced by transforming growth factor beta 1. NEUROBIOLOGY OF DISEASE 2006; 21(-3-):576-586. >>> Hepatocyte growth factor, recomb. human; PBS; BSA; CSF/CNS; Mice; 1007D; 1002; 7,14 days; Animal info (C57BL/6, 10-days old).

P7923 Simard,A.R., Soulet,D., Gowing,G., Julien,J.P., Rivest,S. Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease. Neuron 2006; 49(-4-):489-502. >>> Ganciclovir; Saline; HCL; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); animal info (15-24 weeks old).

P7918 Saavedra,J.M., Armando,I., Bregonzio,C., Juorio,A., Macova,M., Pavel,J., Sanchez-Lemus,E. A centrally acting, anxiolytic angiotensin II AT1 receptor antagonist prevents the isolation stress-induced decrease in cortical CRF1 receptor and benzodiazepine binding. Neuropsychopharmacology 2006; 31(-6-):1123-1134. >>> Candesartan; Saline; sodium carbonate; SC; CSF/CNS; Rat; 14 days; Controls received mp w/ vehicle; animal info (Wistar, 8 weeks old).

P7914 Chen,L., Yamada,K., Nabeshima,T., Sokabe,M. Alpha 7 Nicotinic acetylcholine receptor as a target to rescue deficit in hippocampal UP induction in beta-amyloid infused rats. Neuropharmacology 2006; 50(-2-):254-268. >>> Amyloid protein, beta (1-40); amyloid protein, beta (40-1); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; 11,14 days; Controls received mp w/ vehicle or reverse AB (40-1); no stress (see pg. 255); peptides; neurodegenerative (Alzheimer's disease); animal info (male, KBL Wistar, 280-300 g., 7 weeks old); 35% acetonitrile used.

P7912 Beraud,E., Viola,A., Regaya,I., Confort-Gouny,S., Siaud,P., Ibarrola,D., Le Fur,Y., Barbaria,J., Pellissier,J.F., Sabatier,J.M., Medina,I., Cozzone,P.J. Block of neural Kv1.1 potassium channels for neuroinflammatory disease therapy. Annals of Neurology 2006; 60(-5-):586-596. >>> BgK-F6A; CSF/CNS; Rat; 2001; 2002; 3-5 days; 14-18 days; Controls received mp w/ saline; rats with highest doses of drug had jumping seizures (2 rats), pg. 588; ALZET brain infusion kit 2 used; peptides; animal info (female, Lewis, 6 and 12 wk old).

P7888 da Silva,A.A., Tallam,L.S., Liu,J.K., Hall,J.E. Chronic antidiabetic and cardiovascular actions of leptin: role of CNS and increased adrenergic activity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 291(-5-):R1275-R1282. >>> Leptin; Saline; CSF/CNS; Rat; 2002; 10 days; Controls received IV saline; functionality of mp verified by plasma leptin concentrations; comparison of IV infusion vs. mp; cardiovascular; peptides; animal info (male, Sprague-Dawley, 275-325g.).

P7874 Naert,G., Ixart,G., Tapia-Arancibia,L., Givalois,L. Continuous i.c.v. infusion of brain-derived neurotrophic factor modifies hypothalamic-pituitary-adrenal axis activity, locomotor activity and body temperature rhythms in adult male rats. Neuroscience 2006; 139(-2-):779-789. >>> Brain-derived neurotrophic factor; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle, or no treatment; comparison of acute icv injections vs. mp; no stress (see pg. 782); peptides; animal info (male, Sprague-Dawley, 375g.); mp + tubing primed overnight in 37 celsius saline; telemetric transmitter IP.

P7864 Ricard,J., Salinas,J., Garcia,L., Liebl,D.J. EphrinB3 regulates cell proliferation and survival in adult neurogenesis. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 31(-4-):713-722. >>> Ephrin B3-Fc ligand; PBS; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ Fc fragments; animal info (CD1, Ephrin B3 -/-, wt).

P7861 Ramirez-Castillejo,C., Sanchez-Sanchez,F., ndreu-Agullo,C., Ferron,S.R., roca-Aguilar,J.D., Sanchez,P., Mira,H., Escribano,J., Farinas,I. Pigment epithelium-derived factor is a niche signal for neural stem cell renewal. Nature Neuroscience 2006; 9(-3-):331-339. >>> Pigment epithelium-derived factor; pigment epithelium-derived factor, c-terminal; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (CD1, adult).

P7843 Yamaguchi,Y., Miyashita,H., Tsunekawa,H., Mouri,A., Kim,H.C., Saito,K., Matsuno,T., Kawashima,S., Nabeshima,T. Effects of a novel cognitive enhancer, spiro[imidazo[1,2-a]pyridine-3,2- indan]-2(3H)-one (ZSET1446), on learning impairments induced by amyloid-beta (1-40) in the rat. Journal of Pharmacology and Experimental Therapeutics 2006; 317(-3-):1079-1087. >>> Amyloid protein, beta (1-40); amyloid protein, beta (40-1); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; 15 days; Controls received mp w/ vehicle, or AB (40-1); stability verified by testing in solution at 37 celsius (pg. 1080); neurodegenerative (alzheimer's disease); animal info (male, Wistar, 7 wk. old); "An animal model induced by chronic intracerebroventricular (i.c.v.) infusion of AB is particularly attractive for evaluation of drugs for AD because change is similar to those known for AD patients" (pg. 1080); 35% acetonitrile used.

P7831 Windle,R.J., Gamble,L.E., Kershaw,Y.M., Wood,S.A., Lightman,S.L., Ingram,C.D. Gonadal steroid modulation of stress-induced hypothalamo-pituitary-adrenal activity and anxiety behavior: Role of central oxytocin. Endocrinology 2006; 147(-5-):2423-2431. >>> Oxytocin antagonist; oxytocin antagonist, desGly-; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ saline; replacement therapy (ovariectomy); ALZET brain infusion kit used; peptides; animal info (female, Sprague-Dawley, 225-250g.); mp primed overnight in isotonic saline.

P7824 Moriya,R., Sano,H., Umeda,T., Ito,M., Takahashi,Y., Matsuda,M., Ishihara,A., Kanatani,A., Iwaasa,H. RFamide peptide QRFP43 causes obesity with hyperphagia and reduced thermogenesis in mice. Endocrinology 2006; 147(-6-):2916-2922. >>> Peptide, RFamide QREFP43; PBS; BSA; CSF/CNS; Mice; 2004; 4 weeks; 24 days; Controls received mp w/ vehicle; dose-response (fig. 3); pumps replaced after 2 weeks of vehicle alone; ALZET brain infusion kit used; peptides; post op. care (cefamedin alpha); animal info (male, C57BL/6J, 27-30g., 15-wk old); endocrinology.

P7816 Givalois,L., Naert,G., Tapia-Arancibia,L., Arancibia,S. Involvement of brain-derived neurotrophic factor in the regulation of hypothalamic somatostatin in vivo. Journal of Endocrinology 2006; 188(-3-):425-433. >>> Brain-derived neurotrophic factor, recomb. human; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle, or no treatment; comparison of icv injections vs. mp; peptides; animal info (male, Sprague-Dawley, 260-280g.); mp primed overnight in 37 celsius saline.

P7812 Ma,Q.L., Lim,G.P., Harris-White,M.E., Yang,F.S., Ambegaokar,S.S., Glabe,C.G., Teter,B., Frautschy,S.A., Cole,G.M. Antibodies against beta-amyloid reduce A beta oligomers, glycogen synthase kinase-3 beta activation and tau phosphorylation in vivo and in vitro. Journal of Neuroscience Research 2006; 83(-3-):374-384. >>> Antibody, monoclonal, anti-amyloid protein, beta (1-15); immunoglobulin, IgG2b, mouse; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ mouse IgG2b; post op. care (marcaine); neurodegenerative (Alzheimer's disease); animal info (male, female, 11-12 month old).

P7793 Seto,S., Nagao,S., Ozeki,S., Tetsuo,H., Akahoshi,M., Yano,K. Contribution of central nitric oxide to the regulation of blood pressure and sodium balance in DOCA-salt hypertension. Journal of Cardiovascular Pharmacology 2006; 47(-5-):680-685. >>> L-NMMA; IP; CSF/CNS; Rat; 2ML4; 4 weeks; Controls received mp w/ saline; replacement therapy (nephrectomy); dose-response (fig. 1); enzyme inhibitor (no synthase); cardiovascular; animal info (male, Wistar, 7 wk. old, 240g.); cannula location confirmed by methylene blue staining.

P7782 Glezer,I., Lapointe,A., Rivest,S. Innate immunity triggers oligodendrocyte progenitor reactivity and confines damages to brain injuries. FASEB Journal 2006; 20(-2-):U405-U430. >>> Endotoxin, LPS; MK-801; Saline, sterile; CSF/CNS; Rat; 2004; 3, 7 days; Controls received mp w/ vehicle; immunology; animal info (male, Sprague-Dawley, 250g.).

P7781 Grimaldi,P., Rossi,F. Lack of neurogenesis in the adult rat cerebellum after Purkinje cell degeneration and growth factor infusion. European Journal of Neuroscience 2006; 23(-10-):2657-2668. >>> Epidermal growth factor, recomb. human; fibroblast growth factor, basic; fibroblast growth factor 8b, recomb. mouse; Phosphate buffer; CSF/CNS; Rat; 2002; 1, 7, 14 days; Controls received mp w/ vehicle; peptides; animal info (Wistar albino, 200-250g, 2-3 months old).

P7779 Craft,J.M., Watterson,D.M., Van Eldik,L.J. Human amyloid beta-induced neuroinflammation is an early event in neurodegeneration. Glia 2006; 53(-5-):484-490. >>> Amyloid protein, beta (1-42), human; HEPES; lipoprotein, human high density; CSF/CNS; Mice; 1002; 2 weeks; 28 days; Controls received mp w/ vehicle; peptides; neurodegenerative (Alzheimer's disease); animal info (C57BL/6, 2-3 months old); mp partially coated with paraffin; HDL reduces AB aggregation.

P7762 Jung,K.H., Chu,K., Lee,S.T., Kim,S.J., Sinn,D.I., Kim,S.U., Kim,M., Roh,J.K. Granulocyte colony-stimulating factor stimulates neurogenesis via vascular endothelial growth factor with STAT activation. Brain Research 2006; 1073(--):190-201. >>> Granulocyte colony-stimulating factor, recomb.; Saline; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; no stress (see pg. 199); peptides; animal info (male, Sprague-Dawley, 220-240g).

P7747 Schwenke,D.O., Pearson,J.T., Kangawa,K., Shirai,M. Does central nitric oxide chronically modulate the acute hypoxic ventilatory response in conscious rats?. ACTA PHYSIOLOGICA 2006; 186(-4-):309-318. >>> L-NAME; sydnonimine-hydrochloride, 3-[4-morpholinyl]-; CSF, artificial; CSF/CNS; Rat; 2004; 3 weeks; Controls received mp w/ vehicle; no stress (see pg. 312); enzyme inhibitor (no synthase); post op. care (viccillin); animal info (male, Sprague-Dawley, 8 wk. old, 200-320g.); correct catheter positioning confirmed post mortem w/ Evans blue dye.

P7728 Frielingsdorf,H., Thal,L.J., Pizzo,D.P. The septohippocampal cholinergic system and spatial working memory in the Morris water maze. Behavioural Brain Research 2006; 168(-1-):37-46. >>> Nerve growth factor, recomb. human; CSF, artificial; gentamicin; albumin, rat serum; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; cannula placement confirmed by cresyl violet staining; peptides; animal info (male, Fisher 344, 13-14 wk. old, medial septum lesion, 400g); mp primed 2 days in 37 Celsius saline.

P7719 Mouri,A., Zou,L.B., Iwata,N., Saido,T.C., Wang,D.Y., Wang,M.W., Noda,Y., Nabeshima,T. Inhibition of neprilysin by thiorphan (i.c.v.) causes an accumulation of amyloid beta and impairment of learning and memory. Behavioural Brain Research 2006; 168(-1-):83-91. >>> Thiorphan; Saline; NaOH; ascorbic acid; CSF/CNS; Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; enzyme inhibitor (neprilysin (aka enkephalinase)); neurodegenerative (Alzheimer's disease); animal info (male, wistar, 8 wk. old, 260-300g.); memory; learning.

P7670 Lim,D.A., Suarez-Farinas,M., Naef,F., Hacker,C.R., Menn,B., Takebayashi,H., Magnasco,M., Patil,N., varez-Buylla,A. In vivo transcriptional profile analysis reveals RNA splicing and chromatin remodeling as prominent processes for adult neurogenesis. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 31(-1-):131-148. >>> Ara-C; Saline; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle or no treatment; animal info (CD-1, 2-3 months old); anti-mitotic cytosine arabinoside.

P7666 Crocker,S.J., Hayley,S.P., Smith,P.D., Mount,M.P., Lamba,W.R., Callaghan,S.M., Slack,R.S., Park,D.S. Regulation of axotomy-induced dopaminergic neuron death and c-Jun phosphorylation by targeted inhibition of cdc42 or mixed lineage kinase. Journal of Neurochemistry 2006; 96(-2-):489-499. >>> CEP11004; Kreb's solution; bicarbonate; ringer's solution; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; enzyme inhibitor (mixed lineage kinases [MLK]); Alzet brain infusion kit 2 used; animal info (male, Wistar, 150-175 g, MFB axotomy).

P7659 Joo,Y., Kim,H.S., Woo,R.S., Park,C.H., Shin,K.Y., Lee,J.P., Chang,K.A., Kim,S., Suh,Y.H. Mefenamic acid shows neuroprotective effects and improves cognitive impairment in in vitro and in vivo Alzheimer's disease models. MOLECULAR PHARMACOLOGY 2006; 69(-1-):76-84. >>> Amyloid protein, beta (1-42); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 1007D; 1 week; Alzet brain infusion kit used; neurodegenerative (Alzheimer's disease); animal info (male, Wistar 200-250 g); 35% acetonitrile.

P7640 Zhao,L.X., Ma,Q.L., Calon,F., Harris-White,M.E., Yang,F.S., Lim,G.P., Morihara,T., Ubeda,O.J., Ambegaokar,S., Hansen,J.E., Weisbart,R.H., Teter,B., Frautschy,S.A., Cole,G.M. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nature Neuroscience 2006; 9(-2-):234-242. >>> Peptide, PAK18; peptide, PAK18-R192A; CSF, artificial; CSF/CNS; Mice; 1002; 1 month; 2, 15 days; Controls received mp w/ vehicle or control peptide PAK18-R192A; peptides; neurodegenerative (Alzheimer's disease); animal info (B6D2F1, 11 month old, male); p21-activated kinase (PAK) peptide 18 contains PAK inhibitory domain with cell permeant TAT sequence.

P7618 Park,J.H., Gimbel,D.A., GrandPre,T., Lee,J.K., Kim,J.E., Li,W.W., Lee,D.H.S., Strittmatter,S.M. Alzheimer precursor protein interaction with the Nogo-66 receptor reduces amyloid-beta plaque deposition. Journal of Neuroscience 2006; 26(-5-):1386-1395. >>> Nogo-66 receptor (310) ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS; Mice (transgenic); 2004; 56 days; Controls received mp w/ rat IgG; long-term study; pumps replaced after 28 days; Alzet Brain Infusion Kit 2 used; brain tissue distribution; cyanoacrylate adhesive; neurodegenerative (Alzheimer's disease) animal info (C57BL/6J, APPswe/PSEN or WT, 6-8 months old).

P7614 Malhotra,S., Savitz,S.I., Ocava,L., Rosenbaum,D.M. Ischemic preconditioning is mediated by erythropoietin through PI-3 kinase signaling in an animal model of transient ischemic attack. Journal of Neuroscience Research 2006; 83(-1-):19-27. >>> Erythropoietin receptor, soluble; PBS; BSA; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ vehicle; peptides; ischemia (cerebral); animal info (male, Wistar, 200-225 g, MCA occlusion).

P7585 Day,M., Langston,R.F. Post-training N-methyl-D-aspartate receptor blockade offers protection from retrograde interference but does not affect consolidation of weak or strong memory traces in the water maze. Neuroscience 2006; 137(-1-):19-28. >>> Ap5, D-; CSF, artificial; CSF/CNS; Rat; 1007D; 7, 14 days; Controls received mp w/ vehicle; pumps replaced after 7 days; post op. care (carpofen); animal info (male, Lister-hooded 230-280 g); electrophysiology.

P7584 Darnaudery,M., Perez-Martin,M., Belizaire,G., Maccari,S., Garcia-Segura,L.M. Insulin-like growth factor 1 reduces age-related disorders induced by prenatal stress in female rats. NEUROBIOLOGY OF AGING 2006; 27(-1-):119-127. >>> Insulin-like growth factor I, recomb. human; Saline; CSF/CNS; Rat; 2004; 21 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (female, Sprague-Dawley 24 month old, 2 month old).

P7582 Cardoso,A., Paula-Barbosa,M.M., Lukoyanov,N.V. Reduced density of neuropeptide Y neurons in the somatosensory cortex of old male and female rats: Relation to cholinergic depletion and recovery after nerve growth factor treatment. Neuroscience 2006; 137(-3-):937-948. >>> Dye, methylene blue; nerve growth factor; CSF, artificial; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle or sham surgery; functionality of mp verified by pretesting; Alzet brain infusion kit used; post op. care (antiseptic); animal info (male, female, Wistar 6 month old, 24 month old); Lynch coil filled with NGF, air-oil spacer between mp and catheter, mp filled with dye.

P7561 Sun,Y.J., Jin,K.L., Childs,J.T., Xie,L., Mao,X.O., Greenberg,D.A. Vascular endothelial growth factor-B (VEGFB) stimulates neurogenesis: Evidence from knockout mice and growth factor administration. Developmental Biology 2006; 289(-2-):329-335. >>> Vascular endothelial growth factor-B; CSF, artificial; CSF/CNS; Rat; mice; 1003D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); animal info (mice, 10-12 wk old; male, Sprague-Dawley).

P7547 Ghashghaei,H.T., Weber,J., Pevny,L., Schwab,R., Schwab,M.H., Lloyd,K.C.K., Eisenstat,D.D., Lai,C., Anton,E.S. The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zone. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-6-):1930-1935. >>> Neuregulin 1, GST-; Neuregulin 2, GST-; GST; CSF, artificial; CSF/CNS; Mice; 1003D; 1007D; 7 days; 24 hours; Controls received mp w/ GST; comparison of ICV injections vs. mp; animal info (8 wk old, CD-1ErbB4).

P7518 Wilsey,J., Matheny,M.K., Scarpace,P.J. Oral vanadium enhances the catabolic effects of central leptin in young adult rats. Endocrinology 2006; 147(-1-):493-501. >>> Leptin; CSF, artificial; CSF/CNS; Rat; 2004; 21, 28 days; Controls received mp w/ vehicle; dose-response (fig. 7); ALZET brain infusion kit used; peptides; animal info (F344, brown Norway, male, 3 months old, 357-370 g); mp primed 36 hours in 37 C sterile saline.

P7451 Fekete,C., Singru,P.S., Sanchez,E., Sarkar,S., Christoffolete,M.A., Riberio,R.S., Rand,W.M., Emerson,C.H., Bianco,A.C., Lechan,R.M. Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. Endocrinology 2006; 147(-1-):520-529. >>> Leptin, recomb. mouse; insulin; glucose; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by agent levels in CSF+ serum; peptides; post op. care (bacitracin ointment); animal info (male Sprague-Dawley, 200-250 g); cannula placement confirmed by light microscope.