References on the Intracerebroventricular Administration of Agents to Mice Using ALZET^® Osmotic Pumps

R0240 Bazan,N.G. Omega-3 fatty acids, pro-inflammatory signaling and neuroprotection. CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE 2007; 10(-2-):136-141. >>> Neuroprotectin D1; CSF/CNS (third ventricle); Mice; ; Ischemia (cerebral); neurodegenerative (Alzheimer's disease); see pg. 137.

R0234 Behlke,M.A. Progress towards in vivo use of siRNAs. MOLECULAR THERAPY 2006; 13(-4-):644-670. >>> RNA, small interfering; CSF/CNS (dorsal third ventricle); Mice; 2 weeks; Half-life (p. 649-650), 0.03-6.5 hrs in mice; brain tissue distribution; ALZET mentioned on pg. 659, ref. 206.

R0212 Sahu,A. Leptin signaling in the hypothalamus: emphasis on energy homeostasis and leptin resistance. Frontiers in Neuroendocrinology 2003; 24(-4-):225-253. >>> Leptin, recomb., mouse; CSF, artificial; PBS; CSF/CNS; Rat; mice; 4 weeks; Controls received mp w/ vehicle; p. 237-238.

R0153 Meier,J.J., Gallwitz,B., Schmidt,W.E., Nauck,M.A. Glucagon-like peptide 1 as a regulator of food intake and body weight: therapeutic perspectives. European Journal of Pharmacology 2002; 440(--):269-279. >>> Glucagon-like peptide-1; CSF/CNS; mice; ; continuous ICV infusion via minipump mentioned on p. 272; numerous delivery methods; brain tissue distribution; peptides.

R0138 Seidman,S., Eckstein,F., Grifman,M., Soreq,H. Antisense technologies have a future fighting neurodegenerative diseases. Antisense&Nucleic Acid Drug Development 1999; 9(--):333-340. >>> Oligonucleotide, antisense; CSF/CNS; Rat; mice; ; Antisense; osmotic pumps mentioned on p. 337; neurodegenerative.

P9192 Singh,M.K., Elefteriou,F., Karsenty,G. Cocaine and amphetamine-regulated transcript may regulate bone remodeling as a circulating molecule. Endocrinology 2008; 149(-8-):3933-3941. >>> CART; PBS; CSF/CNS (third ventricle); Mice (transgenic); 28 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; cyanoacrylate adhesive; animal info (CART -/-, female, 5 months old); compound also known as cocaine and amphetamine-regulated transcript, a mediator of the leptin regulation of bone mass.

P9176 Knauf,C., Cani,P.D., it-Belgnaoui,A., Benani,A., Dray,C., Cabou,C., Colom,A., Uldry,M., Rastrelli,S., Sabatier,E., Godet,N., Waget,A., Penicaud,L., Valet,P., Burcelin,R. Brain glucagon-like peptide 1 signaling controls the onset of high-fat diet-induced insulin resistance and reduces energy expenditure. Endocrinology 2008; 149(-10-):4768-4777. >>> Exendin-9; SC; CSF/CNS; Mice; 2004; 4 weeks; Controls received SC mp w/ Exendin-9; animal info (C57BL6/J, 12 wks old).

P9174 Trotter-Mayo,R.N., Roberts,M.R. Leptin acts in the periphery to protect thymocytes from glucocorticoid-mediated apoptosis in the absence of weight loss. Endocrinology 2008; 149(-10-):5209-5218. >>> Leptin; IP; CSF/CNS; Mice; 1002; 12 days; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ob/ob, db/db, wt, C57BL/6).

P9120 Shen,Q., Wang,Y., Kokovay,E., Lin,G., Chuang,S.M., Goderie,S.K., Roysam,B., Temple,S. Adult SVZ stem cells lie in a vascular niche: A quantitative analysis of niche cell-cell interactions. Cell Stem Cell 2008; 3(-3-):289-300. >>> Antibody, GoH3; CSF, artificial; CSF/CNS; Mice (transgenic); 1007D; 6 days; Controls received mp w/ immunoglobulin G, rat; animal info (2-3 months old, Swiss Webster, GFAP-GFP).

P9118 Knauf,C., Cani,P.D., Kim,D.H., Iglesias,M.A., Chabo,C., Waget,A., Colom,A., Rastrelli,S., Delzenne,N.M., Drucker,D.J., Seeley,R.J., Burcelin,R. Role of central nervous system glueagon-like peptide-1 reeeptors in enterie glueose sensing. Diabetes 2008; 57(-10-):2603-2612. >>> Exendin (9-39); CSF/CNS; Mice; ; ALZET brain infusion kit; animal info (male, C57BL/6, 12 wks old, GLP-1Rc KO); cannula connected to tygon tubing filled with artificial CSF; dental cement used.

P9074 Xue,B.J., Zhao,Y.Z., Johnson,A.K., Hay,M. Central estrogen inhibition of angiotensin II-induced hypertension in male mice and the role of reactive oxygen species. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1025-H1032. >>> Tempol; estradiol, 17B-; ICI-182780; SC; CSF/CNS; Mice; 1002; ; ALZET brain infusion kit used; peptides; animal info (male, C57BL/6, 12-16 wks old); location of cannula was verified histologically post mortem.

P9059 Yang,D.S., Kumar,A., Stavrides,P., Peterson,J., Peterhoff,C.M., Pawlik,M., Levy,E., Cataldo,A.M., Nixon,R.A. Neuronal apoptosis and autophagy cross talk in aging PS/APP mice, a model of Alzheimer's disease. American Journal of Pathology 2008; 173(-3-):665-681. >>> Leupeptin; HEPES, buffer; CSF/CNS; Mice (transgenic); 2004; 2 weeks; Enzyme inhibitor (cysteine protease); cyanoacrylate adhesive; animal info (6 months old, PS/APP, or wt); neurodegenerative (Alzheimer's Disease); pumps primed in sterile saline at 37 degree Celsius for 48 hours.

P9054 Hao,Q., Su,H., Marchuk,D.A., Rola,R., Wang,Y.Q., Liu,W.Z., Young,W.L., Yang,G.Y. Increased tissue perfusion promotes capillary dysplasia in the ALK1-deficient mouse brain following VEGF stimulation. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-6-):H2250-H2256. >>> Hydralazine; nicardipine; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ saline; animal info (adult, male, ALK1 +/-); fig. 1 illustrates cannula placement; cannula placement verified by CBF measurements.

P8952 Dolev,I., Michaelson,D.M. The nucleation growth and reversibility of Amyloid-beta deposition in vivo. JOURNAL OF ALZHEIMERS DISEASE 2006; 10(-2-3-):291-301. >>> Thiorphan, DL-; CSF, artificial; ascorbic acid; CSF/CNS; Mice (transgenic); 2004; 4 weeks; Controls received mp w/ vehicle; enzyme inhibitor (neprilysin); animal info (male, C57BL/6, ApoE3 tg, ApoE4 tg, wt, 5 months old); neurodegenerative (Alzheimer's disease).

P8917 Bartolomucci,A., Rigamonti,A.E., Bulgarelli,I., Torsello,A., Locatelli,V., Pavone,F., Levi,A., Possenti,R., Muller,E.E., Moles,A. Chronic intracerebroventricular TLQP-21 delivery does not modulate the GH/IGF-1-axis and muscle strength in mice. GROWTH HORMONE&IGF RESEARCH 2007; 17(-4-):342-345. >>> TLQP-21, synthetic; CSF, artificial; CSF/CNS; Mice; 1002; 14 days; ALZET brain infusion kit 1 used; peptides; animal info (male, Swiss CD1, 30-35 g.); behavioral testing (grip strength).

P8853 Hook,V.Y.H., Kindy,M., Hook,G. Inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant beta-secretase site of the amyloid precursor protein. Journal of Biological Chemistry 2008; 283(-12-):7745-7753. >>> CAO74Me; E64d; Saline; Me2SO; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; no stress (see pg. 7747); enzyme inhibitor (cysteine protease); ALZET brain infusion kit 3 used; peptides; animal info (male, London APP, Swedish/London APP; 6, 12 months old); neurodegenerative (Alzheimer's disease); "Animals remained healthy and were observed to have normal eating, body weight, grooming + general appearance during the inhibitor treatment period. Of the 96 animals used in this study, none showed postoperative complications, such as infections; also, none died." (p. 7747).

P8829 Zaheer,A., Zaheer,S., Thangavel,R., Wu,Y.H., Sahu,S.K., Yang,B.L. Glia maturation factor modulates beta-amyloid-induced glial activation, inflammatory cytokine/chemokine production and neuronal damage. Brain Research 2008; 1208(-;-):192-203. >>> Amyloid protein, beta (1-42), human; Saline; CSF/CNS; Mice; 2004; 28 days; Controls received mp w/ vehicle; peptides; animal info (C57BL/6, wt, GMF-KO, 5-6 months old); neurodegenerative (Alzheimer's disease).

P8807 Sato,S., Hanada,R., Kimura,A., Abe,T., Matsumoto,T., Iwasaki,M., Inose,H., Ida,T., Mieda,M., Takeuchi,Y., Fukumoto,S., Fujita,T., Kato,S., Kangawa,K., Kojima,M., Shinomiya,K., Takeda,S. Central control of bone remodeling by neuromedin U. Nature Medicine 2007; 13(-10-):1234-1240. >>> Neuromedin U-23, rat; leptin, human; CSF/CNS; Mice; 28 days; Controls received mp w/ PBS; peptides; animal info (C57BL/6J, wt, LepOb, Nmu -/-, Adrb2 -/-, male, female, 3 months old).

P8794 Louis,S.A., Rietze,R.L., Deleyrolle,L., Wagey,R.E., Thomas,T.E., Eaves,A.C., Reynolds,B.A. Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay. Stem Cells 2008; 26(-4-):988-996. >>> Epidermal growth factor; ara-c; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; cyanoacrylate adhesive; animal info (adult, C57BL/6).

P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P8751 Ghia,J.E., Blennerhassett,P., Collins,S.M. Impaired parasympathetic function increases susceptibility to inflammatory bowel disease in a mouse model of depression. Journal of Clinical Investigation 2008; 118(-6-):2209-2218. >>> Reserpine; Acetic acid; CSF/CNS; Mice; 14 days; Controls received mp w/ vehicle; dose-response (Fig. 1); comparison of IP injection vs. mp; no stress (see pg. 2210); antihypertensive; cyanoacrylate adhesive; animal info (male, female, C57BL/6, op/op, wt, 7-9 wks old, colitis, vagotomy); animal model of depression; plastics one cannula used; PE-60 used.

P8746 Ma,Q.L., Harris-White,M.E., Ubeda,O.J., Simmons,M., Beech,W., Lim,G.P., Teter,B., Frautschy,S.A., Cole,G.M. Evidence of A beta and transgene-dependent defects in ERK-CREB signaling in Alzheimer's models. Journal of Neurochemistry 2007; 103(-4-):1594-1607. >>> Antibody, monoclonal anti-amyloid peptide B (1-15); immunoglobulin IgG2b; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ non-specific mouse IgG2b; animal info (Tg2576, Tg-; 12, 22 months old); neurodegenerative (Alzheimer's disease); "AB vaccine or anti-AB antibodies are still among the most promising treatments for AD." (p. 1605).

P8681 Grattan,D.R., Jasoni,C.L., Liu,X.H., Anderson,G.M., Herbison,A.E. Prolactin regulation of gonadotropin-releasing hormone neurons to suppress luteinizing hormone secretion in mice. Endocrinology 2007; 148(-9-):4344-4351. >>> Prolactin, ovine; CSF/CNS; Mice; 1002; 7 days; Serum levels taken, peptides, animal info (adult, female, C57BL6/J, 6-8 weeks, 24-28g, OVX).

P8660 Soliz,J., Gassmann,M., Joseph,V. Soluble erythropoietin receptor is present in the mouse brain and is required for the ventilatory acclimatization to hypoxia. JOURNAL OF PHYSIOLOGY-LONDON 2007; 583(-1-):329-336. >>> Erythropoietin receptor, soluble; Phosphate buffer; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male C57/BL6, 3 months old, hypoxia).

P8646 Kuwata,K., Nishida,N., Matsumoto,T., Kamatari,Y.O., Hosokawa-Muto,J., Kodama,K., Nakamura,H.K., Kimura,K., Kawasaki,M., Takakura,Y., Shirabe,S., Takata,J., Kataoka,Y., Katamine,S. Hot spots in prion protein for pathogenic conversion. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-29-):11921-11926. >>> GN8; Glucose; saline; SC; CSF/CNS; Mice; ; Controls received mp w/ vehicle; animal info (4 wk old).

P8645 Adachi,K., Mirzadeh,Z., Sakaguchi,M., Yamashita,T., Nikolcheva,T., Gotoh,Y., Peltz,G., Gong,L., Kawase,T., varez-Buylla,A., Okano,H., Sawamoto,K. beta-Catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone. Stem Cells 2007; 25(-11-):2827-2836. >>> Ro3303544; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; enzyme inhibitor (protein kinase); animal info (adult, male, C57BL/6, 9 wks); GSK3-beta is a protein kinase.

P8644 Hamill,C.E., Caudle,W.M., Richardson,J.R., Yuan,H., Pennell,K.D., Greene,J.G., Miller,G.W., Traynelis,S.F. Exacerbation of Dopaminergic terminal damage in a mouse model of Parkinson's disease by the g-protein-coupled receptor protease-activated receptor 1. MOLECULAR PHARMACOLOGY 2007; 72(-3-):653-664. >>> BMS-200261; Hepes, buffered saline; CSF/CNS; Mice; 1003D; 2 days; Controls received mp w/ vehicle; animal info (male, C57BL/6, PAR1 +/-); neurodegenerative (Parkinson's Disease); BMS-200261 is a PAR1 antagonist (protease activated receptor); "The pumps were stored overnight at 37oC in sterile saline (0.9% NaCl) to prime drug release." (p. 654); "Placement was verified in a subset of animals by injection of 0.5 ul of Evans blue." (p. 654).

P8632 Han,G.P., Li,L., Kosugi,I., Kawasaki,H., Tsuchida,T., Miura,K., Tsutsui,Y. Enhancement of susceptibility of adult mouse brain to cytomegalovirus infection by infusion of epidermal growth factor. Journal of Neuroscience Research 2007; 85(-13-):2981-2990. >>> Epidermal growth factor, mouse; PBS; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; peptides; animal info (female, BALB/c; 10, 25, and 70 wks old).

P8626 Cho,S.R., Benraiss,A., Chmielnicki,E., Samdani,A., Economides,A., Goldman,S.A. Induction of neostriatal neurogenesis slows disease progression in a transgenic murine model of Huntington disease. Journal of Clinical Investigation 2007; 117(-10-):2889-2902. >>> Ara C; Saline; CSF/CNS; Mice (transgenic); 1002; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; ALZET brain infusion kit 3 used; animal info (5-9 weeks old); neurodegenerative (Huntington's disease).

P8622 Senechal,Y., Kelly,P.H., Cryan,J.F., Natt,F., Dev,K.K. Amyloid precursor protein knockdown by siRNA impairs spontaneous alternation in adult mice. Journal of Neurochemistry 2007; 102(-6-):1928-1940. >>> RNA, small interfering; RNAi buffer, isotonic; CSF/CNS (dorsal third ventricle); Mice; 1002; 2 weeks; Controls received mp w/ vehicle or control siRNA; no stress (see pg. 1934); animal info (male, C57BL/6JICO, 10-12 weeks old, 23-26 g); neurodegenerative (Alzheimer's disease); siRNA targets: amyloid precursor protein (APP), GFP, APP-mismatch, or non targeting control.

P8560 Plum,L., Rother,E., Muenzberg,H., Wunderlich,F.T., Morgan,D.A., Hampel,B., Shanabrough,M., Janoschek,R., Koenner,A.C., Alber,J., Suzuki,A., Krone,W., Horvath,T.L., Rahmouni,K., Bruening,J.C. Enhanced leptin-stimulated Pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metabolism 2007; 6(-6-):431-445. >>> Leptin; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Peptides; animal info (male).

P8515 Ishii,T., Muranaka,R., Tashiro,O., Nishimura,M. Chronic intracerebroventricular administration of anti-neuropeptide Y antibody stimulates starvation-induced feeding via compensatory responses in the hypothalamus. Brain Research 2007; 1144(--):91-100. >>> Antibody, rabbit, anti-neuropeptide Y; Saline; CSF/CNS; Mice; 2002; 13 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; ALZET brain infusion kit 3 used; animal info (male, ddy, 7 weeks old, 35-40 g).

P8513 Gomori,A., Ishihara,A., Ito,M., Matsushita,H., Ito,M., Mashiko,S., Iwaasa,H., Matsuda,M., Bednarek,M.A., Qian,S., MacNeil,D.J., Kanatani,A. Blockade of MCH1 receptor signalling ameliorates obesity and related hepatic steatosis in ovariectomized mice. Br.J.Pharmacol. 2007/7; 151(-6-):900-908. >>> Melanin-concentrating hormone 1, receptor antagonist; Propylene glycol; water, distilled; CSF/CNS; Mice; 2004; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks of vehicle delivery; ALZET brain infusion kit used; post op. care (cefamezin-a); animal info (female, C57BL/6J, 12 weeks old, female Mch1r KD, 14-16 weeks old, 22 g); cannula placement confirmed with Evans Blue dye; obesity.

P8507 Stranahan,A.M., Arumugam,T.V., Cutler,R.G., Lee,K., Egan,J.M., Mattson,M.P. Diabetes impairs hippocampal function through glucocorticoid-mediated effects on new and mature neurons. Nat.Neurosci. 2008/3; 11(-3-):309-317. >>> Ara-C; CSF/CNS; Mice; 1002; ; Diabetes; animal info (2 months old, wild-type); Ara-C is an anti-mitotic drug.

P8500 Xue,B.J., Pamidimukkala,J., Lubahn,D.B., Hay,M. Estrogen receptor-alpha mediates estrogen protection from angiotensin II- induced hypertension in conscious female. American Journal of Physiology-Heart and Circulatory Physiology 2007; 292(-4-):H1770-H1776. >>> Angiotensin II; Estradiol, 17B-; ICI-182,780; CSF/CNS; SC; Mice; 1002; ; Blood pressure measured; cardiovascular; ALZET brain infusion kit used; peptides; animal info (OVX, WT, and ER knockout); CNS group delivered E2 + ICI.

P8464 Zhao,M., Momma,S., Delfani,K., Carlen,M., Cassidy,R.M., Johansson,C.B., Brismar,H., Shupliakov,O., Frisen,J., Janson,A.M. Evidence for neurogenesis in the adult mammalian substantia nigra. Proc.Natl.Acad.Sci.U.S.A 2003/6/24; 100(-13-):7925-7930. >>> Uridine, bromodeoxy-; thymidine, 3H; Ara-C; Saline, radio-isotopes; CSF/CNS; IP; Mice; 1003D; 1002; 2004; 2,3,10,14,21 days; Comparison of oral administration vs. IP injections; animal info (male, C57BL/6, 2-20 months old); neurodegenerative (Parkinson's disease); "the blood brain barrier and or peripheral metabolism limit the availability of BrdUrd in the brain." (p. 7929).

P8428 Iwashita,A., Muramatsu,Y., Yamazaki,T., Muramoto,M., Kita,Y., Yamazaki,S., Mihara,K., Moriguchi,A., Matsuoka,N. Neuroprotective efficacy of the peroxisome proliferator-activated receptor delta-selective Agonists in vitro and in vivo. Journal of Pharmacology and Experimental Therapeutics 2007; 320(-3-):1087-1096. >>> L-165041; GW501516; PEG 300; saline; DMSO; CSF/CNS; Rat; mice; 1003D; 1007D; 2, 6 days; Controls received mp w/ vehicle; dose-response (fig. 6); cardiovascular; ischemia (cerebral); animal info (male, Wistar, 9-10 wks old, 250-280 g.; male, C57/BL6, 9-10 wks old, 19-22g.); neurodegenerative (Parkinson's disease); Right middle cerebral artery occlusion; 30% DMSO.

P8409 Prada,C.M., Garcia-Alloza,M., Betensky,R.A., Zhang-Nunes,S.X., Greenberg,S.M., Bacskai,B.J., Frosch,M.P. Antibody-mediated clearance of amyloid-beta peptide from cerebral amyloid angiopathy revealed by quantitative in vivo imaging. Journal of Neuroscience 2007; 27(-8-):1973-1980. >>> Antibody, anti-amyloid protein beta (10D5); antibody, anti-human tau (16B5); Dye, indocyanine green; CSF/CNS; Mice (transgenic); 2002; 14 days; Controls received mp w/ anti-Tau AB; comparison of single CNS injection vs. mp; animal info (Tg2576, 10-13 months old); neurodegenerative (Alzheimer's disease, CAA); permanent cranial window for serial imaging with multiphoton laser-scanning microscopy; "with continous infusion of anti-AB antibody, the clearance of CAA was more robust and continued throughout the duration of the treatment." (pg. 1978).

P8331 Joppa,M.A., Gogas,K.R., Foster,A.C., Markison,S. Central infusion of the melanocortin receptor antagonist agouti-related peptide (AgRP(83-132)) prevents cachexia-related symptoms induced by radiation and colon-26 tumors in mice. Peptides 2007; 28(-3-):636-642. >>> Agouti-related peptide (83-132); Saline, sterile; CSF/CNS; Mice; 1003D; 72 hours; Controls received mp w/ vehicle; cancer (colon adenocarcinoma); peptides; animal info (male, BALB/C, 6 weeks old, 22 grams); Research Diets D12689B.

P8322 Sakaguchi,M., Shingo,T., Shimazaki,T., Okano,H.J., Shiwa,M., Ishibashi,S., Oguro,H., Ninomiya,M., Kadoya,T., Horie,H., Shibuya,A., Mizusawa,H., Poirier,F., Nakauchi,H., Sawamoto,K., Okano,H. A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-18-):7112-7117. >>> Antibody, anti-galectin-1; immunoglobulin G, rabbit; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle or rabbit IgG; peptides; animal info (male, 1295J wt or galectin-1 -/-, 8 weeks old).

P8316 Ohta,S., Gregg,C., Weiss,S. Pituitary adenylate cyclase-activating polypeptide regulates forebrain neural stem cells and neurogenesis in vitro and in vivo. Journal of Neuroscience Research 2006; 84(-6-):1177-1186. >>> Pituitary adenylate cyclase-activating polypeptide; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; peptides; animal info (CD1, 6-7 wks old).

P8276 Rustamzadeh,E., Hall,W.A., Todhunter,D.A., Vallera,V.D., Low,W.C., Liu,H.Y., Panoskaltsis-Mortari,A., Vallera,D.A. Intracranial therapy of glioblastoma with the fusion protein DTAT in immunodeficient mice. International Journal of Cancer 2006; 120(-2-):411-419. >>> DTAT; Saline; CSF/CNS; Mice; 1007D; ; Controls received mp w/ vehicle; dose-response (table 1); comparison of bolus ICV injections vs. mp; cancer (glioblastoma); toxicology; peptides; animal info (C57BL/6, female, 12 weeks old); diptheria toxin; "delivery of DTAT resulted in about a 16 fold increase in maximum tolerated dose." (p. 411); "The results of this experiment indicate that the toxicity of uPAR can be reduced by CED (mp) delivery." (p. 416).

P8266 Urushitani,M., bou Ezzi,S., Julien,J.P. Therapeutic effects of immunization with mutant superoxide dismutase in mice models of amyotrophic lateral sclerosis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-7-):2495-2500. >>> Antibody, mouse anti hSOD1; antibody, rabbit polyclonal anit-hSOD1-FITC; Saline; CSF/CNS; Mice; 2004; 16, 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; stability verified by Western Blot w/ residual volume AB after 18 days infusion; brain tissue distribution; animal info (G93A SOD1, 85 days old); neurodegenerative (ALS).

P8155 Burcelin,R., Kamohara,S. Acute stimulation of glucose metabolism in mice by leptin treatment. Nature 1997/1/1; 389(--):374-377. >>> Leptin; CSF, artificial; CSF/CNS (third ventricle); Mice; 10 days; Controls received mp w/ PBS; comparison of acute IV infusion vs. mp; CSF pumps replaced with leptin mp after 7 days; peptides; animal info (C57BL/6J +/+ lean, 25 grams); tissue perfusion (ventricle).

P8153 Bauer,S., Patterson,P.H. Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. Journal of Neuroscience 2006; 26(-46-):12089-12099. >>> Ara-C; leukemia inhibitory factor, recomb. mouse; epidermal growth factor, recomb. human; Saline; PBS; BSA; CSF/CNS; Mice; 1003D; 1007D; 1, 3, 6, 7, days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 or 3 used; peptides; animal info (adult, male, C57BL/6J, 2-6 months old).

P8119 Knauf,C., Rieusset,J., Foretz,M., Cani,P.D., Uldry,M., Hosokawa,M., Martinez,E., Bringart,M., Waget,A., Kersten,S., Desvergne,B., Gremlich,S., Wahli,W., Seydoux,J., Delzenne,N.M., Thorens,B., Burcelin,R. Peroxisome proliferator-activated receptor alpha null mice have increased white adipose tissue glucose utilization, GLUT4, and fat mass: Role in liver and brain. Endocrinology 2006; 147(-9-):4067-4078. >>> Wy-14643; DMSO; saline; CSF/CNS; Mice; 7 days; Controls received mp w/ vehicle; comparison of icv 3 hr. acute infusion vs. mp; no stress (see p.4072); Alzet brain infusion kit used; animal info (C57BL/6, wt or female PPAR -/-; 12-14 wks old, 20-25g.); 50% DMSO; endocrinology.

P8111 Jackson,E.L., Garcia-Verdugo,J.M., Gil-Perotin,S., Roy,M., Quinones-Hinojosa,A., VandenBerg,S., varez-Buylla,A. PDGFR-alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 2006; 51(-2-):187-199. >>> Platelet-derived growth factor-AA; Saline; BSA; CSF/CNS; Mice; 6, 14 days; Controls received mp w/ vehicle; comparison of icv injections vs. mp; peptides; animal info (CD-1, GFAP-Tva, 2-4 months old).

P7973 Bartolomucci,A., La Corte,G., Possenti,R., Locatelli,V., Rigamonti,A.E., Torsello,A., Bresciani,E., Bulgarelli,I., Rizzi,R., Pavone,F., D'Amato,F.R., Severini,C., Mignogna,G., Giorgi,A., Schinina,M.E., Elia,G., Brancia,C., Ferri,G.L., Conti,R., Ciani,B., Pascucci,T., Dell'Omo,G., Muller,E.E., Levi,A., Moles,A. TLQP-21, a VGF-derived peptide, increases energy expenditure and prevents the early phase of diet-induced obesity. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-39-):14584-14589. >>> TLQP-21, synthetic; CSF, artificial; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ vehicle; stability verified by HPLC after 14 days at 37 C; ALZET brain infusion kit 1 used; peptides; animal info (male, CD1); obesity.

P7925 Tada,T., Zhan,H., Tanaka,Y., Hongo,K., Matsumoto,K., Nakamura,T. Intraventricular administration of hepatocyte growth factor treats mouse communicating hydrocephalus induced by transforming growth factor beta 1. NEUROBIOLOGY OF DISEASE 2006; 21(-3-):576-586. >>> Hepatocyte growth factor, recomb. human; PBS; BSA; CSF/CNS; Mice; 1007D; 1002; 7,14 days; Animal info (C57BL/6, 10-days old).

P7923 Simard,A.R., Soulet,D., Gowing,G., Julien,J.P., Rivest,S. Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease. Neuron 2006; 49(-4-):489-502. >>> Ganciclovir; Saline; HCL; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); animal info (15-24 weeks old).

P7864 Ricard,J., Salinas,J., Garcia,L., Liebl,D.J. EphrinB3 regulates cell proliferation and survival in adult neurogenesis. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 31(-4-):713-722. >>> Ephrin B3-Fc ligand; PBS; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ Fc fragments; animal info (CD1, Ephrin B3 -/-, wt).

P7861 Ramirez-Castillejo,C., Sanchez-Sanchez,F., ndreu-Agullo,C., Ferron,S.R., roca-Aguilar,J.D., Sanchez,P., Mira,H., Escribano,J., Farinas,I. Pigment epithelium-derived factor is a niche signal for neural stem cell renewal. Nature Neuroscience 2006; 9(-3-):331-339. >>> Pigment epithelium-derived factor; pigment epithelium-derived factor, c-terminal; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (CD1, adult).

P7824 Moriya,R., Sano,H., Umeda,T., Ito,M., Takahashi,Y., Matsuda,M., Ishihara,A., Kanatani,A., Iwaasa,H. RFamide peptide QRFP43 causes obesity with hyperphagia and reduced thermogenesis in mice. Endocrinology 2006; 147(-6-):2916-2922. >>> Peptide, RFamide QREFP43; PBS; BSA; CSF/CNS; Mice; 2004; 4 weeks; 24 days; Controls received mp w/ vehicle; dose-response (fig. 3); pumps replaced after 2 weeks of vehicle alone; ALZET brain infusion kit used; peptides; post op. care (cefamedin alpha); animal info (male, C57BL/6J, 27-30g., 15-wk old); endocrinology.

P7812 Ma,Q.L., Lim,G.P., Harris-White,M.E., Yang,F.S., Ambegaokar,S.S., Glabe,C.G., Teter,B., Frautschy,S.A., Cole,G.M. Antibodies against beta-amyloid reduce A beta oligomers, glycogen synthase kinase-3 beta activation and tau phosphorylation in vivo and in vitro. Journal of Neuroscience Research 2006; 83(-3-):374-384. >>> Antibody, monoclonal, anti-amyloid protein, beta (1-15); immunoglobulin, IgG2b, mouse; PBS; CSF/CNS; Mice (transgenic); 1002; 14 days; Controls received mp w/ mouse IgG2b; post op. care (marcaine); neurodegenerative (Alzheimer's disease); animal info (male, female, 11-12 month old).

P7779 Craft,J.M., Watterson,D.M., Van Eldik,L.J. Human amyloid beta-induced neuroinflammation is an early event in neurodegeneration. Glia 2006; 53(-5-):484-490. >>> Amyloid protein, beta (1-42), human; HEPES; lipoprotein, human high density; CSF/CNS; Mice; 1002; 2 weeks; 28 days; Controls received mp w/ vehicle; peptides; neurodegenerative (Alzheimer's disease); animal info (C57BL/6, 2-3 months old); mp partially coated with paraffin; HDL reduces AB aggregation.

P7706 Mashiko,S., Ishihara,A., Iwaasa,H., Sano,H., Oda,Z., Ito,J., Yumoto,M., Okawa,M., Suzuki,J., Fukuroda,T., Jitsuoka,M., Morin,N.R., MacNeil,D.J., Van der Ploeg,L.H., Ihara,M., Fukami,T., Kanatani,A. Characterization of neuropeptide Y (NPY) Y5 receptor-mediated obesity in mice: chronic intracerebroventricular infusion of D-Trp34NPY. Endocrinology 2003/5; 144(-5-):1793-1801. >>> Neuropeptide Y, D-Trp34; PBS; BSA; CSF/CNS; Mice; 2001; 2002; 13-21 days; Controls received mp w/ vehicle; dose-response (fig. 1); pumps replaced after 7-14 days; ALZET Brain Infusion kit used; peptides; post op. care (cefamedin); animal info (C57BL/6J, 9-12 weeks old, male); cannula placement confirmed by Evans blue dye injection.

P7670 Lim,D.A., Suarez-Farinas,M., Naef,F., Hacker,C.R., Menn,B., Takebayashi,H., Magnasco,M., Patil,N., varez-Buylla,A. In vivo transcriptional profile analysis reveals RNA splicing and chromatin remodeling as prominent processes for adult neurogenesis. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 31(-1-):131-148. >>> Ara-C; Saline; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle or no treatment; animal info (CD-1, 2-3 months old); anti-mitotic cytosine arabinoside.

P7640 Zhao,L.X., Ma,Q.L., Calon,F., Harris-White,M.E., Yang,F.S., Lim,G.P., Morihara,T., Ubeda,O.J., Ambegaokar,S., Hansen,J.E., Weisbart,R.H., Teter,B., Frautschy,S.A., Cole,G.M. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nature Neuroscience 2006; 9(-2-):234-242. >>> Peptide, PAK18; peptide, PAK18-R192A; CSF, artificial; CSF/CNS; Mice; 1002; 1 month; 2, 15 days; Controls received mp w/ vehicle or control peptide PAK18-R192A; peptides; neurodegenerative (Alzheimer's disease); animal info (B6D2F1, 11 month old, male); p21-activated kinase (PAK) peptide 18 contains PAK inhibitory domain with cell permeant TAT sequence.

P7634 Andersson,N., Islander,U., Egecioglu,E., Loef,E., Swanson,C., Moverare-Skrtic,S., Sjoegren,K., Lindberg,M.K., Carlsten,H., Ohlsson,C. Investigation of central versus peripheral effects of estradiol in ovariectomized mice. Journal of Endocrinology 2005; 187(-2-):303-309. >>> Estradiol, 17B-; Cyclodextrin, hydroxypropyl, beta; CSF/CNS; SC; Mice; 1002; ; Controls received mp w/ vehicle; ALZET Brain Infusion Kit used; multiple pumps per animal (2) used; animal info (F, OVX, C57BL/6, 8 weeks old); SC and ICV administration performed concomitantly.

P7630 Bauer,S., Patterson,P.H. The cell cycle-apoptosis connection revisited in the adult brain. Journal of Cell Biology 2005; 171(-4-):641-650. >>> Uridine, bromodeoxy-; Saline; CSF/CNS; Mice; 1003D; 24 hours; ALZET Brain Infusion Kit 2 used; animal info (2-4 month old, C57BL/6).

P7618 Park,J.H., Gimbel,D.A., GrandPre,T., Lee,J.K., Kim,J.E., Li,W.W., Lee,D.H.S., Strittmatter,S.M. Alzheimer precursor protein interaction with the Nogo-66 receptor reduces amyloid-beta plaque deposition. Journal of Neuroscience 2006; 26(-5-):1386-1395. >>> Nogo-66 receptor (310) ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS; Mice (transgenic); 2004; 56 days; Controls received mp w/ rat IgG; long-term study; pumps replaced after 28 days; Alzet Brain Infusion Kit 2 used; brain tissue distribution; cyanoacrylate adhesive; neurodegenerative (Alzheimer's disease) animal info (C57BL/6J, APPswe/PSEN or WT, 6-8 months old).

P7615 Kitazawa,M., Oddo,S., Yamasaki,T.R., Green,K.N., LaFerla,F.M. Lipopolysaccharide-induced inflammation exacerbates tau pathology by a cyclin-dependent kinase 5-mediated pathway in a transgenic model of Alzheimer's disease. Journal of Neuroscience 2005; 25(-39-):8843-8853. >>> Roscovitine; DMSO; CSF/CNS; Mice; mice (transgenic); 1002; 2 weeks; Controls received mp w/ vehicle; enzyme inhibitor (cdK5); ALZET Brain Infusion Kit 3 used; neurodegenerative (Alzheimer's disease); animal info (3xTg-AD, WT, 4 month old, female); 50% DMSO.

P7607 Fernandez,A.M., Carro,E.M., Lopez-Lopez,C., Torres-Aleman,I. Insulin-like growth factor I treatment for cerebellar ataxia: Addressing a common pathway in the pathological cascade?. BRAIN RESEARCH REVIEWS 2005; 50(-1-):134-141. >>> Insulin-like growth factor I; SC; CSF/CNS; Rat; mice; 1 month; 2 weeks; Peptides; neurodegenerative (cerebellar ataxia); Review, pg. 138, ref #21; "This long-term delivery system increases therapeutically available IGF-1 at least 30-fold as compared to single subcutaneous injections" (p. 138).

P7588 Gammon,C.M., Freeman,GM Jr, Xie,W.H., Petersen,S.L., Wetsel,W.C. Regulation of gonadotropin-releasing hormone secretion by cannabinoids. Endocrinology 2005; 146(-10-):4491-4499. >>> CP 55, 940; CSF, artificial; CSF/CNS (third ventricle); Mice; ; Controls received mp w/ vehicle; animal info (female,C57BL/6J); mp primed 12 hrs in saline; cannula location confirmed w/ methylene blue.

P7580 Poluektova,L., Meyer,V., Walters,L., Paez,X., Gendelman,H.E. Macrophage-induced inflammation affects hippocampal plasticity and neuronal development in a murine model of HIV-1 encephalitis. Glia 2005; 52(-4-):344-353. >>> Uridine, bromodeoxy-; Saline, physiological; CSF/CNS; Mice (SCID); 2004; 7, 28 days; Animal info (SCID, 4 wk old, male).

P7568 Pieper,A.A., Wu,X.L., Han,T.W., Estill,S.J., Dang,Q.Y., Wu,L.C., Reece-Fincanon,S., Dudley,C.A., Richardson,J.A., Brat,D.J., McKnight,S.L. The neuronal PAS domain protein 3 transcription factor controls FGF-mediated adult hippocampal neurogenesis in mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2005; 102(-39-):14052-14057. >>> Fibroblast growth factor, basic; CSF, artificial; CSF/CNS; Mice; 1007D; 4 days; Controls received mp w/ vehicle; animal info (male, 3 month old); also known as FGF2.

P7561 Sun,Y.J., Jin,K.L., Childs,J.T., Xie,L., Mao,X.O., Greenberg,D.A. Vascular endothelial growth factor-B (VEGFB) stimulates neurogenesis: Evidence from knockout mice and growth factor administration. Developmental Biology 2006; 289(-2-):329-335. >>> Vascular endothelial growth factor-B; CSF, artificial; CSF/CNS; Rat; mice; 1003D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); animal info (mice, 10-12 wk old; male, Sprague-Dawley).

P7547 Ghashghaei,H.T., Weber,J., Pevny,L., Schwab,R., Schwab,M.H., Lloyd,K.C.K., Eisenstat,D.D., Lai,C., Anton,E.S. The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zone. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-6-):1930-1935. >>> Neuregulin 1, GST-; Neuregulin 2, GST-; GST; CSF, artificial; CSF/CNS; Mice; 1003D; 1007D; 7 days; 24 hours; Controls received mp w/ GST; comparison of ICV injections vs. mp; animal info (8 wk old, CD-1ErbB4).

P7544 Daniels,D.J., Lenard,N.R., Etienne,C.L., Law,P.Y., Roerig,S.C., Portoghese,P.S. Opioid-induced tolerance and dependence in mice is modulated by the distance between pharmacophores in a bivalent ligand series. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2005; 102(-52-):19208-19213. >>> Morphine; DN-20; MDAN-16; MDAN-17; MDAN-18; MDAN-19; MDAN-20; MDAN21; MA19; Saline, sterile; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; tolerance; dependance; cyanoacrylate adhesive; animal info (male, ICR, 25-30 g); mp primed in 37 C saline overnight; MDAN is mu-delta-agonist-antagonist series to opioid receptors of bivalent ligands that contain different length spacers (16-21 atoms) and pharmocophores derived from naltrindole; MA is mu-agonist monovalent control; DN is delta antagonist monovalent control.

P7521 Ophir,G., Amariglio,N., Jacob-Hirsch,J., Elkon,R., Rechavi,G., Michaelson,D.M. Apolipoprotein E4 enhances brain inflammation by modulation of the NF-kappaB signaling cascade. NEUROBIOLOGY OF DISEASE 2005; 20(-3-):709-718. >>> Endotoxin, LPS; CSF/CNS; Mice (transgenic); ; Controls received PBS; Alzet brain infusion kit used; neurodegenerative (Alzheimer's disease); animal info (male, 4 month old, ApoE3, ApoE4 transgenic); 5 min infusion with syringe pump and BIK, no mp used.

P7480 Craft,J.M., Watterson,D.M., Marks,A., Van Eldik,L.J. Enhanced susceptibility of S-100B transgenic mice to neuroinflammation and neuronal dysfunction induced by intracerebroventricular infusion of human beta-amyloid. Glia 2005; 51(-3-):209-216. >>> Amyloid protein, beta (1-42), human oligomeric; HEPES buffer; lipoprotein, high density; CSF/CNS; Mice (transgenic); 1002; 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; brain tissue distribution; peptides; neurodegenerative (Alzheimer's disease); mp partially coated with paraffin to reduce infusion rate and extend duration.

P7465 Lenard,N.R., Roerig,S.C. Development of antinociceptive tolerance and physical dependence following morphine i.c.v. infusion in mice. European Journal of Pharmacology 2005; 527(-1-3-):71-76. >>> Morphine sulfate; Saline, sterile; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; comparison of sc pellets vs. mp; no stress (see pg. 73); tolerance; dependence; cyanoacrylate adhesive; post op. care (antibacterial ointment); animal info (male, 28-45 g); mp primed in sterile saline 37C overnight.

P7436 Parras,C.M, Galli,R, Britz,O, Soares,S. Mash 1 specifies neurons and oligodendrocytes in the postnatal brain. The European Molecular Biology Organization Journal 2004; 23(--):4495-4505. >>> Ara-C; Saline; CSF/CNS; Mice; 6 days; Animal info (male, 8-10 weeks old).

P7393 Thakker,D.R., Natt,F., Huesken,D., van der Putten,H., Maier,R., Hoyer,D., Cryan,J.F. siRNA-mediated knockdown of the serotonin transporter in the adult mouse brain. Molecular Psychiatry 2005; 10(-8-):782-789. >>> RNA, small interfering; citalopram; RNA, mm; RNAi buffer, isotonic; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; gene therapy; downregulation of SERT; animal info (male, BALB/C, 19-29 g); "maximally effective RNAi response requires 2 weeks of siRNA infusion." P. 783; behavioral study.

P7366 Kitaichi,K., Fukuda,M., Nakayama,H., Aoyama,N., Ito,Y., Fujimoto,Y., Takagi,K., Takagi,K., Hasegawa,T. Behavioral changes following antisense oligonucleotide-induced reduction of organic cation transporter-3 in mice. Neuroscience Letters 2005; 382(-1-2-):195-200. >>> Oligonucleotide, antisense; oligonucleotide, antisense, scrambled; CSF/CNS (third ventricle); Mice; 1002; 7 days; Antisense (OCT3); "The dose and infusion period of AS&SERAS used in this study did not produce any abnormal behavior or weight loss." p. 196.

P7248 Lakka,S.S., Gondi,C.S., Dinh,D.H., Olivero,W.C., Gujrati,M., Rao,V.H., Sioka,C., Rao,J.S. Specific interference of urokinase-type plasminogen activator receptor and matrix metalloproteinase-9 gene expression induced by double-stranded RNA results in decreased invasion, tumor growth, and angiogenesis in gliomas. Journal of Biological Chemistry 2005; 280(-23-):21882-21892. >>> RNA, small interfering; RNA, small interfering, uPAR; RNA, small interfering, MMP-9; CSF/CNS; Mice (nude); 1002; 2 weeks; Controls received mp w/ PBS or empty vector; cancer (glioblastoma); siRNA is within a plasmid vector (pUM vector); antiangiogenesis; gene therapy.

P7245 Mashiko,S., Ishihara,A., Gomori,A., Moriya,R., Ito,M., Iwaasa,H., Matsuda,M., Feng,Y., Shen,Z., Marsh,D.J., Bednarek,M.A., MacNeil,D.J., Kanatani,A. Antiobesity effect of a melanin-concentrating hormone 1 receptor antagonist in diet-induced obese mice. Endocrinology 2005; 146(-7-):3080-3086. >>> Melanin-concentrating hormone 1 receptor antagonist; Propylene glycol; CSF, artificial; CSF/CNS; CSF/CNS (third ventricle); Mice; 1007D; 2002; 2004; 3, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 1 or 4 weeks of vehicle; ALZET brain infusion kit used; peptides; cyanoacrylate adhesive; post op. care (cefamedin); DEXA; cannula placement confirmed by Evans blue dye injection; animal info (C57BL/6); pumps used in lateral ventricle for one group of animals, and third ventricle for others. Third ventricle group used Plastics One cannulae.

P7190 Holmberg,J., Armulik,A., Senti,K.A., Edoff,K., Spalding,K., Momma,S., Cassidy,R., Flanagan,J.G., Frisen,J. Ephrin-A2 reverse signaling negatively regulates neural progenitor proliferation and neurogenesis. GENES&DEVELOPMENT 2005; 19(-4-):462-471. >>> Ephrin-A2-Fc; Ephrin-B2-Fc; EphA7-Fc; Immunoglobulin G, goat anti-human; Ephrin-B1-Fc; PBS; CSF/CNS; Mice; 1007D; 3 days; Controls received mp w/ Fc; peptides; animal info (adult,male, C57BL/6).

P7182 Osuchowski,M.F., Edwards,G.L., Sharma,R.P. Fumonisin B1-induced neurodegeneration in mice after intracerebroventricular infusion is concurrent with disruption of sphingolipid metabolism and activation of proinflammatory signaling. Neurotoxicology 2005; 26(-2-):211-221. >>> Fumonisin B1; Saline; SC; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; dose-response (fig 3); no stress (see pg. 214); enzyme inhibitor (ceramide synthase); toxicology; mp primed overnight; animal info (female, 7-8 weeks old, BALB/C).

P7137 Baker,S.A., Baker,K.A., Hagg,T. D3 dopamine receptors do not regulate neurogenesis in the subventricular zone of adult mice. NEUROBIOLOGY OF DISEASE 2005; 18(-3-):523-527. >>> DPAT, 7-hydroxy-U-99194A maleate; Saline; CSF/CNS; CSF/CNS (neostriatum); Rat; mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response; comparison of IP injections vs. mp; stability verified by BrdU labeling; cyanoacrylate adhesive used.

P7110 Ona,V.O, Li,M.W., Vonsattel,J.P.G., Andrews,L.J., Khan,S.Q., Chung,W.M., Frey,A.S., Menon,A.S., Li,X.J., Stieg,P.E, Yuan,J., Penney,J.B., Young,A.B., Cha,J.H.L., Friedlander,R.M. Inhibition of caspase-1 slows disease progression in a mouse model of Huntington's disease. Nature 1999; 399(--):263-267. >>> zVAD-fmk; zFA-fmk; CSF/CNS; Mice; 4 weeks; Controls received mp w/ zFA-fmk; enzyme inhibitor (caspase).

P7099 Benn,S.C., Ay,I., Bastia,E., Chian,R.J., Celia,S.A., Pepinsky,R.B., Fishman,P.S., Brown,RH Jr, Francis,J.W. Tetanus toxin fragment C fusion facilitates protein delivery to CNS neurons from cerebrospinal fluid in mice. Journal of Neurochemistry 2005; 95(-4-):1118-1131. >>> Toxin, tetanus fragment C; tempol, toxin tetanus fragment C; CSF/CNS; Mice; 2001; 7 days; Stability verified by incubation at 37C for 7 days; brain tissue distribution; post op. care (bupivicaine and betadine in wound); animal info (C57BL/6, male, 25-30 g); dental cement used for cannula adhesion, agent also know as TTC; compounds were also distributed to further brain sites such as the hypothalamus and the thalamus.

P7068 Harris-White,M.E., Balverde,Z., Lim,G.P., Kim,P., Miller,S.A., Hammer,H. Role of LRP in TGFbeta2-mediated neuronal uptake of A beta and effects on memory. Journal of Neuroscience Research 2004; 77(-2-):217-228. >>> Amyloid protein, beta; transforming growth factor-B2; receptor-associated protein; HEPES buffer; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; stability verified by incubation at 37 degree Celsius for 2,4 weeks; peptides; RAP is a LRP (lipoprotein receptor-related protein) antagonist; memory.

P6989 Enwere,E., Shingo,T., Gregg,C., Fujikawa,H., Ohta,S., Weiss,S. Aging results in reduced epidermal growth factor receptor signaling, diminished olfactory neurogenesis, and deficits in fine olfactory discrimination. Journal of Neuroscience 2004; 24(-38-):8354-8365. >>> Epidermal growth factor, human recomb.; Ara-C; Saline; albumin, mouse serum; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; peptides.

P6961 Stamatovic,S.M., Shakui,P., Keep,R.F., Moore,B.B., Kunkel,S.L., van Rooijen,N., Andjelkovic,A.V. Monocyte chemoattractant protein-1 regulation of blood-brain barrier permeability. Journal of Cerebral Blood Flow and Metabolism 2005; 25(-5-):593-606. >>> Monocyte chemoattractant protein-1; PBS; CSF/CNS; Mice; 1003D; 1007D; 3,7 days; Animal info (CD-1 or CCR2, C57BL/C, 8-10 weeks old).

P6952 Henry,M., Ghibaudi,L., Gao,J., Hwa,J.J. Energy metabolic profile of mice after chronic activation of central NPY Y1, Y2, or Y5 receptors. Obesity Research 2005; 13(-1-):36-47. >>> Neuropeptide Y; Y1 agonist; Y5 agonist; Saline; water; ascorbic acid; CSF/CNS; Mice; 2001; 6 days; Controls received mp w/ vehicle; peptides; mice received a two week recovery period; guide cannula used and secured with tissue adhesive; animal info (6 week old, C57BL/6, 25-30 grams).

P6923 Kawakami,K., Kawakami,M., Kioi,M., Husain,S.R., Puri,R.K. Distribution kinetics of targeted cytotoxin in glioma by bolus or convection-enhanced delivery in a murine model. Journal of Neurosurgery 2004; 101(-6-):1004-1011. >>> Interleukin-13, PE38; Albumin, human serum; PBS; CSF/CNS; Mice (nude); 1007D; 7 days; Comparison of CNS injections vs. mp; cancer (glioma); ALZET brain infusion kit; cyanoacrylate adhesive.

P6916 Zhang,M., Li,J., Chakrabarty,P., Bu,B.T., Vincent,I. Cyclin-dependent kinase inhibitors attenuate protein hyperphosphorylation, cytoskeletal lesion formation, and motor defects in Niemann-Pick type C mice. American Journal of Pathology 2004; 165(-3-):843-853. >>> Roscovitine; olomoucine; iso-olomoucine; DMSO; CSF/CNS; Mice; 1002; 2, 4 weeks; Pumps replaced every 2 weeks for 4 week infusions; enzyme inhibitor (CDK); neurodegenerative (Alzheimer's disease, Amyotrophic Lateral Sclerosis, Niemann-Pick Type C disease); lynch coil used to accomodate 75% DMSO.

P6882 Craft,J.M., Watterson,D.M., Frautschy,S.A., Van Eldik,L.J. Aminopyridazines inhibit beta-amyloid-induced glial activation and neuronal damage in vivo. NEUROBIOLOGY OF AGING 2004; 25(-10-):1283-1292. >>> Amyloid protein, beta (1-42), human; HEPES buffer; lipoprotein, high density; CSF/CNS; Mice; 1002; 28 days; Controls received mp w/ vehicle; peptides; mp partially coated with paraffin wax to reduce infusion rate; HDL used to reduce amyloid-beta aggregation; Plastics One cannula used; neurodegenerative disease (Alzheimer's).

P6872 Dolev,I., Michaelson,D.M. A nontransgenic mouse model shows inducible amyloid-beta (Abeta) peptide deposition and elucidates the role of apolipoprotein E in the amyloid cascade. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-38-):13909-13914. >>> Thiorphan; CSF, artificial; ascorbic acid; CSF/CNS; Mice; mice (transgenic); 2004; 1 month; 2,4 weeks; Controls received mp w/ vehicle; enzyme inhibitor (neprilysin); ALZET brain infusion kit used; cyanoacrylate adhesive (Loctite 454); post op. care (oxytetracycline); neurodegenerative disease (Alzheimer's).

P6856 Frielingsdorf,H., Schwarz,K., Brundin,P., Mohapel,P. No evidence for new dopaminergic neurons in the adult mammalian substantia nigra. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-27-):10177-10182. >>> Uridine, bromodeoxy-; brain-derived neurotrophic factor; CSF/CNS; Rat; mice; 2002; 2004; 10,21 days; Brain tissue distribution.

P6843 Zimmerman,M.C., Lazartigues,E., Sharma,R.V., Davisson,R.L. Hypertension caused by angiotensin II infusion involves increased superoxide production in the central nervous system. Circulation Research 2004; 95(-2-):210-216. >>> Angiotensin II; losartan; SC; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ saline; cardiovascular; peptides; multiple pumps per animal (2); AT1 receptor antagonists.

P6769 Thakker,D.R., Natt,F., Huesken,D., Maier,R., Mueller,M., van der Putten,H., Hoyer,D., Cryan,J.F. Neurochemical and behavioral consequences of widespread gene knockdown in the adult mouse brain by using nonviral RNA interference. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-49-):17270-17275. >>> RNA, small interfering; GBR 12909; RNAi buffer; CSF/CNS (dorsal third ventricle); Mice; 1007D; 1002; 1,2 weeks; Controls received mp w/ vehicle; antisense (EGFP, DAT, scrambled sequence); Plastics One cannula used; "Results highlight the temporal effects of siRNA, requiring a constant minipump-mediated infusion in the dorsal third ventricle for a stable and bilateral knockdown of gene expression in the brain." (p. 17275); gene therapy.

P6647 Caille,I., Allinquant,B., Dupont,E., Bouillot,C., Langer,A., Mueller,U., Prochiantz,A. Soluble form of amyloid precursor protein regulates proliferation of progenitors in the adult subventricular zone. Development 2004; 131(-9-):2173-2181. >>> Amyloid precursor protein-Fc, soluble; batimastat; immunoglobulin G, human; amyloid precursor like protein 2-Fc, soluble; oligonucleotide, antisense penetratin; AraC; Sodium chloride; saline; CSF/CNS; Mice; 1003D; 1007D; 3,6 days; Controls received mp w/ vehicle; antisense (APP, APLP2); brain tissue distribution; batimastat inhibits clearage of several membrane proteins; neurodegenerative disease (Alzheimer's).

P6627 Doh-ura,K., Ishikawa,K., Murakami-Kubo,I., Sasaki,K., Mohri,S., Race,R., Iwaki,T. Treatment of transmissible spongiform encephalopathy by intraventricular drug infusion in animal models. JOURNAL OF VIROLOGY 2004; 78(-10-):4999-5006. >>> Quinacrine; chloroquine; amphotericin B; E-64d; pentosan polysulfate; PBS; water, distilled; DMSO; SC; CSF/CNS; Rat; mice; dog (mongrel); 2 months; 4 weeks; Controls received mp w/ vehicle; dose-response (fig. 2); no stress (see pg. 5004); stress/adverse reaction: (see pg. 5002) PPS at highest dose (20mg/kg/day) showed hemorrhage in the SC area surrounding the mp; enzyme inhibitor (cysteine protease [E-64d]);.

P6619 Suzukawa,J., Omori,K., Yang,L., Inagaki,C. Continuous administration of antisense oligonucleotides to c-fos reduced the development of seizure susceptibility after ethacrynic acid-induced seizure in mice. Neuroscience Letters 2003; 349(-1-):21-24. >>> Oligodeoxynucleotide, phosphorothioate antisense; oligodexynucleotide, phosphorothioate sense; HEPES buffer; sodium chloride; CSF/CNS; Mice; 2002; 9 days; Controls received mp w/ sense ODN; antisense (C-fos); ALZET brain infusion kit used; after experiment, the settled position of the cannula was confirmed.

P6618 Lakka,S.S., Gondi,C.S., Yanamandra,N., Olivero,W.C., Dinh,D.H., Gujrati,M., Rao,J.S. Inhibition of cathepsin B and MMP-9 gene expression in glioblastoma cell line via RNA interference reduces tumor cell invasion, tumor growth and angiogenesis. ONCOGENE 2004; 23(-27-):4681-4689. >>> RNA, small interfering; human cytomegalovirus promoter; Empty vector; CSF/CNS; Mice; 2004; 5 weeks; Cancer (glioblastoma); siRNA against mmp-9 and cathepsin; antiangiogenesis; gene therapy.

P6601 McMahon,B.M., Stewart,J., Fauq,A., Younkin,S., Younkin,L., Richelson,E. Peptide nucleic acids targeted to the amyloid precursor protein. Journal of Molecular Neuroscience 2003; 20(-3-):261-265. >>> Peptide nucleic acid, antisense; peptide nucleic acid, missense; CSF/CNS; Mice; 2004; 28 days; Controls received mp w/ saline; comparison of ICV injections vs. mp; antisense (beta-site APP-clearing enzyme) (BACE); neurodegenerative disease (Alzheimer's).

P6583 Suzuki,R., Tobe,K., Aoyama,M., Inoue,A., Sakamoto,K., Yamauchi,T., Kamon,J., Kubota,N., Terauchi,Y., Yoshimatsu,H., Matsuhisa,M., Nagasaka,S., Ogata,H., Tokuyama,K., Nagai,R., Kadowaki,T. Both insulin signaling defects in the liver and obesity contribute to insulin resistance and cause diabetes in Irs2-/- mice. Journal of Biological Chemistry 2004; 279(-24-):25039-25049. >>> Leptin; CSF/CNS; Mice; 1003D; ; Controls received mp w/ saline; peptides.

P6580 Lindley,T.E., Doobay,M.F., Sharma,R.V., Davisson,R.L. Superoxide is involved in the central nervous system activation and sympathoexcitation of myocardial infarction-induced heart failure. Circulation Research 2004; 94(-3-):402-409. >>> Losartan; CSF/CNS; Mice; 1007D; 1 week; Controls received mp w/ vehicle; cardiovascular.

P6518 Murakami-Kubo,I., Doh-ura,K., Ishikawa,K., Kawatake,S., Sasaki,K., Kira,J., Ohta,S., Iwaki,T. Quinoline derivatives are therapeutic candidates for transmissible spongiform encephalopathies. JOURNAL OF VIROLOGY 2004; 78(-3-):1281-1288. >>> Quinine; quinoline, Bi-; DMSO; CSF/CNS; Mice; 2004; 4 weeks; Controls received mp w/ vehicle; dose-response (fig.3); comparison of IP injections vs. mp; ALZET brain infusion kit used; DMSO 25%.

P6495 Raposinho,P.D., Pedrazzini,T., White,R.B., Palmiter,R.D., Aubert,M.L. Chronic neuropeptide Y infusion into the lateral ventricle induces sustained feeding and obesity in mice lacking either Npy1r or Npy5r expression. Endocrinology 2004; 145(-1-):304-310. >>> Neuropeptide Y, porcine; Phosphate buffer; sodium chloride; ascorbic acid; BSA; CSF/CNS; Mice; 2001; 7 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg.305) initial weight loss from surgery/anesthesia; ALZET brain infusion kit 2 used; peptides; cyanoacrylate adhesive (Loctite 454).

P6489 Asilmaz,E., Cohen,P., Miyazaki,M., Dobrzyn,P., Ueki,K., Fayzikhodjaeva,G., Soukas,A.A., Kahn,C.R., Ntambi,J.M., Socci,N.D., Friedman,J.M. Site and mechanism of leptin action in a rodent form of congenital lipodystrophy. Journal of Clinical Investigation 2004; 113(-3-):414-424. >>> Leptin, recomb. mouse; SC; CSF/CNS (third ventricle); Mice; 12 days; Controls received mp w/ PBS; dose-response (fig.6); peptides.

P6453 Cui,Y.X., Huang,L., Elefteriou,F., Yang,G.Q., Shelton,J.M., Giles,J.E., Oz,O.K., Pourbahrami,T., Lu,C.Y.H., Richardson,J.A., Karsenty,G., Li,C. Essential role of STAT3 in body weight and glucose homeostasis. MOLECULAR AND CELLULAR BIOLOGY 2004; 24(-1-):258-269. >>> Leptin, recomb. human; Saline; CSF/CNS (third ventricle); Mice (knockout, transgenic); 2004; 28 days; ALZET brain infusion kit 2; peptides; obesity; diabetes.

P6418 Mercer,A., Roennholm,H., Holmberg,J., Lundh,H., Heidrich,J., Zachrisson,O., Ossoinak,A., Frisen,J., Patrone,C. PACAP promotes neural stem cell proliferation in adult mouse brain. Journal of Neuroscience Research 2004; 76(-2-):205-215. >>> PACAP38; uridine, bromodeoxy-; Saline; serum albumin, mouse; CSF/CNS; Mice; 1007D; 3.5,7 days.

P6364 Gregg,C., Weiss,S. Generation of functional radial glial cells by embryonic and adult forebrain neural stem cells. Journal of Neuroscience 2003; 23(-37-):11587-11601. >>> Fibroblast growth factor-2; epidermal growth factor; transforming growth factor; Saline; serum albumin; CSF/CNS; Mice; 1007D; 6,12 days; Pumps replaced at day 7 for 12 d EGF infusions.

P6362 Maslov,A.Y., Barone,T.A., Plunkett,R.J., Pruitt,S.C. Neural stem cell detection, characterization, and age-related changes in the subventricular zone of mice. Journal of Neuroscience 2004; 24(-7-):1726-1733. >>> Ara-C; Saline; CSF/CNS; Mice; 1007D; 7 days; ALZET brain infusion kit.

P6284 Elefteriou,F., Takeda,S., Ebihara,K., Magre,J., Patano,N., Kim,C.A., Ogawa,Y., Liu,X., Ware,S.M., Craigen,W.J., Robert,J.J., Vinson,C., Nakao,K., Capeau,J., Karsenty,G. Serum leptin level is a regulator of bone mass. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-9-):3258-3263. >>> Leptin; CSF/CNS; Mice (transgenic); ; ALZET brain infusion kit 2 used; peptides; recomb. human leptin used; no pump listed.

P6283 Marr,R.A., Guan,H.J., Rockenstein,E., Kindy,M., Gage,F.H., Verma,I., Masliah,E., Hersh,L.B. Neprilysin regulates amyloid beta peptide levels. Journal of Molecular Neuroscience 2003; 22(-1-2-):5-11. >>> Phosphoramidon; thiorphan; CSF/CNS; Mice (transgenic); ; Neurodegenerative (Alzheimer's disease); pump model not listed; neurotoxicity; agents are enzyme inhibitors (neprilysin).

P6239 Sinnayah,P., Blair-West,J.R., McBurnie,M.I., McKinley,M.J., Oldfield,B.J., Rivier,J., Vale,W.W., Walker,L.L., Weisinger,R.S., Denton,D.A. The effect of urocortin on ingestive behaviours and brain Fos immunore activity in mice. European Journal of Neuroscience 2003; 18(-2-):373-382. >>> CSF, artificial; urocortin, rat; CSF, artificial; saline; CSF/CNS (third ventricle); Mice; 2001; 2002; 21,28 days; Controls received mp w/ vehicle; dose-reponse; comparison of SC infusions vs. ICV mp; pumps replaced after 1 week and again after 2 weeks; no stress (see pg. 374); good methods p. 374; 7 days aCSF; mp replaced 7 or 14 days either UCN or aCSF; mp replaced 7 days aCSF for 28 days total.

P6238 Morshead,C.M., Garcia,A.D., Sofroniew,M.V., van der Kooy,D. The ablation of glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells. European Journal of Neuroscience 2003; 18(-1-):76-84. >>> Ganciclovir; Saline, physiological; CSF/CNS; Mice (transgenic); 1007D; 3 days; Controls received mp w/ vehicle.

P6232 Hohmann,J.G., Krasnow,S.M., Teklemichael,D.N., Clifton,D.K., Wynick,D., Steiner,R.A. Neuroendocrine profiles in galanin-overexpressing and knockout mice. Neuroendocrinology 2003; 77(-6-):354-366. >>> Galanin; CSF, artificial; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume in pump; comparison of ICV injections vs. mp; ALZET brain infusion kit used; peptides; cyanoacrylate adhesive.

P6196 Butchbach,M.E.R., Guo,H., Lin,C.L.G. Methyl-beta-cyclodextrin but not retinoic acid reduces EAAT3-mediated glutamate uptake and increases GTRAP3-18 expression. Journal of Neurochemistry 2003; 84(-4-):891-894. >>> Cyclodextrin, methyl-beta-; CSF, artifical; CSF/CNS; Mice; 1007D; 60 hours; Controls received mp w/ vehicle.

P6128 Cancedda,L., Putignano,E., Impey,S., Maffei,L., Ratto,G.M., Pizzorusso,T. Patterned vision causes CRE-mediated gene expression in the visual cortex through PKA and ERK. Journal of Neuroscience 2003; 23(-18-):7012-7020. >>> U0126; Rp-8-cl-cAMPS; DMSO; CSF/CNS; Rat; mice (transgenic); 1007D; 7 days; Enzyme inhibitor (PKA and extracellular singal-regulated kinase); U0126 (ERK inhibitor) and Rp-8-cl-cAMPS (PKA inhibitor) were dissolved in 1% DMSO.

P6110 Takahashi,K., Mizuarai,S., Araki,H., Mashiko,S., Ishihara,A., Kanatani,A., Itadani,H., Kotani,H. Adiposity elevates plasma MCP-1 levels leading to the increased CD11b-positive monocytes in mice. Journal of Biological Chemistry 2003; 278(-47-):46654-46660. >>> Neuropeptide Y; monocyte chemoattractant protein-1; PBS; BSA; CSF/CNS; Mice; 2002; 2 weeks; Controls received mp w/ vehicle; mcp-1 plasma levels taken; ALZET brain infusion kit used; peptides; obesity; 7-14 day recovery period after surgery; pumps filled with PBS during recovery; monocyte chemoattractant protein-1 is also known as MCP-1.

P6002 Smith,P.D., Crocker,S.J., Jackson-Lewis,V., Jordan-Sciutto,K.L., Hayley,S., Mount,M.P., O'Hare,M.J., Callaghan,S., Slack,R.S., Przedborski,S., Anisman,H., Park,D.S. Cyclin-dependent kinase 5 is a mediator of dopaminergic neuron loss in a mouse model of Parkinson's disease. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2003; 100(-23-):13650-13655. >>> Flavopiridol; CSF, artificial; CSF/CNS; Mice; 1007D; 14 days; Controls received mp w/ vehicle; neurodegenerative (Parkinson's disease).

P5948 Prass,K., Scharff,A., Ruscher,K., Loewl,D., Muselmann,C., Victorov,I., Kapinya,K., Dirnagl,U., Meisel,A. Hypoxia-induced stroke tolerance in the mouse is mediated by erythropoietin. Stroke 2003; 34(-8-):1981-1986. >>> Erythropoietin, soluble receptor; PBS; BSA; CSF/CNS; Mice; 1003D; 64 hours; Controls received mp w/ vehicle; peptides; ischemia (cerebral); human EPO used; 0.1%BSA used in vehicle.

P5874 Marcheselli,V.L., Hong,S., Lukiw,W.J., Tian,X.H., Gronert,K., Musto,A., Hardy,M., Gimenez,J.M., Chiang,N., Serhan,C.N., Bazan,N.G. Novel docosanoids inhibit brain ischemia-reperfusion-mediated leukocyte infiltration and pro-inflammatory gene expression. Journal of Biological Chemistry 2003; 278(-44-):43807-43817. >>> 10,17S-docosatriene; CSF/CNS (third ventricle); Mice; 48 hours; Controls received mp w/ vehicle; good methods p. 43808; ischemia (cerebral); pump model not stated; 10,17S-docosatriene is a lipid docosanoid.

P5869 Takeda,S., Elefteriou,F., Levasseur,R., Liu,X.Y., Zhao,L.P., Parker,K.L., Armstrong,D., Ducy,P., Karsenty,G. Leptin regulates bone formation via the sympathetic nervous system. Cell 2002; 111(-3-):305-317. >>> Leptin; MTII; CSF/CNS (third ventricle); Mice; 2004; 28 days; Controls received mp w/ PBS; ALZET brain infusion kit 2 used; peptides; leptin was human recomb.

P5859 Camargo,F., Erickson,R.P., Garver,W.S., Hossain,G.S., Carbone,P.N., Heidenreich,R.A., Blanchard,J. Cyclodextrins in the treatment of a mouse model of Niemann-Pick C disease. Life Sci 2001; 70(-2-):131-142. >>> Cyclodextrin, 2-hydroxypropyl-ß-; Saline, sterile; CSF/CNS; Mice (knockout); 2004; 28 days; stress/adverse reaction: (see pg.139) mice were active&broke the cemented cannulae away from the skull; ALZET brain infusion kit 2 used (per Dr. Erickson); 2-Hydroxypropyl-ß-cyclodextrin is (HPBCD); methylene blue dye infused (probably not by pump) after cyclodextrin infusion to confirm the intraventricular location; delivery route somewhat confusing - paper refers to the route alternately as intrathecal and intraventricular; neurodegenerative (Niemann-Pick Type C disease).

P5823 Liberatore,G.T., Samson,A., Bladin,C., Schleuning,W.D., Medcalf,R.L. Vampire bat salivary plasminogen activator (desmoteplase) - A unique fibrinolytic enzyme that does not promote neurodegeneration. Stroke 2003; 34(-2-):537-543. >>> Tissue-type plasminogen activator; Desmoteplase; PBS; CSF/CNS; Mice (knockout); 1007D; 7 days; Controls received mp w/ vehicle; peptides; Desmoteplase is a vampire bat salivary plasminogen activator (a fibrinolytic enzyme); neurodegeneration; tissue plasminogen activator is also known as alteplase.

P5801 Shingo,T., Sorokan,S.T., Shimazaki,T., Weiss,S. Erythropoietin regulates the in vitro and in vivo production of neuronal progenitors by mammalian forebrain neural stem cells. J Neurosci 2001; 21(-24-):9733-9743. >>> Erythropoietin; antibody, rabbit anti-EPO neutralizing; rabbit IgG; Saline; albumin, mouse serum; rat serum; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; peptides; Erythropoietin (EPO) Recomb. Human, antibody&IgG were dissolved in 0.9% saline with 1 mg/ml mouse serum albumin; pump/cannula schematic (p. 9739 fig A).

P5681 Teramoto,T., Qu,J.H., Plumier,J.C., Moskowitz,M.A. EGF amplifies the replacement of parvalbumin-expressing striatal inter neurons after ischemia. Journal of Clinical Investigation 2003; 111(-8-):1125-1132. >>> Epidermal growth factor; PBS; BSA; CSF/CNS; Mice; 1007D; 7 days; peptides; ALZET brain infusion kit 2 used; 1mg/ml of albumin was added as carrier to solution to prevent degradation; ischemia (cerebral).

P5647 Ophir,G., Meilin,S., Efrati,M., Chapman,J., Karussis,D., Roses,A., Michaelson,D.M. Human apoE3 but not apoE4 rescues impaired astrocyte activation in apo E null mice. NEUROBIOLOGY OF DISEASE 2003; 12(-1-):56-64. >>> Endotoxin, LPS; PBS; CSF/CNS; Mice (transgenic); ; No pump used in study; ALZET Brain Infusion Kit used.

P5639 Matsuoka,Y., Saito,M., LaFrancois,J., Saito,M., Gaynor,K., Olm,V., Wang,L.L., Casey,E., Lu,Y.F., Shiratori,C., Lemere,C., Duff,K. Novel therapeutic approach for the treatment of Alzheimer's disease by peripheral administration of agents with an affinity to beta-amyloid. Journal of Neuroscience 2003; 23(-1-):29-33. >>> Ganglioside; PBS; CSF/CNS; Mice (transgenic); 2 weeks; Comparison of IP injections vs. mp; ALZET brain infusion kit 2 used (per Dr. La Francois); neurodegenerative (Alzheimer's disease).

P5605 Gomori,A., Ishihara,A., Ito,M., Mashiko,S., Matsushita,H., Yumoto,M., Ito,M., Tanaka,T., Tokita,S., Moriya,M., Iwaasa,H., Kanatani,A. Chronic intracerebroventricular infusion of MCH causes obesity in mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2003; 284(-3-):E583-E588. >>> Melanin-concentrating hormone; Propylene glycol; CSF/CNS; Mice; 2001; 14 days; Controls received mp/ w vehicle; peptides; ALZET brain infusion kit used; 1st pump with vehicle only to allow for recovery (7-14 days), then the pump was replaced for MCH infusion; obesity.

P5599 Sanchez,I., Mahlke,C., Yuan,J.Y. Pivotal role of oligomerization in expanded polyglutamine neurodegenerative disorders. Nature 2003; 421(-6921-):373-379. >>> Dye, Congo Red; PBS; DMSO; CSF/CNS; Mice (transgenic); 2004; 28 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used (confirmed by Dr. Sanchez); neurodegenerative (Huntington's disease); 2% DMSO used.

P5598 Crocker,S.J., Smith,P.D., Jackson-Lewis,V., Lamba,W.R., Hayley,S.P., Grimm,E., Callaghan,S.M., Slack,R.S., Melloni,E., Przedborski,S., Robertson,G.S., Anisman,H., Merali,Z., Park,D.S. Inhibition of calpains prevents neuronal and behavioral deficits in an MPTP mouse model of Parkinson's disease. Journal of Neuroscience 2003; 23(-10-):4081-4091. >>> Epidermal growth factor; Ganciclovir; Saline; BSA; PBS; CSF/CNS; Mice; 1007D; 1002; ; Controls received mp w/ vehicle; Gene therapy; peptides; EGF was diluted in BSA-containing saline and infused for 7 hours, or 6 days in 1007D pumps; Ganciclovir was diluted in PBS and infused for 6 days via 1002 pumps.

P5539 Chen,H.C., Ladha,Z., Farese,RV Jr  Endocrinology 2002; 143(-8-):2893-2898. >>> Leptin; SC; CSF/CNS; Mice; 1002; 3, 6 days; Dose-response (graph, p. 2897); peptides; ALZET brain infusion kit 2 used.

P5392 Martens,D.J., Seaberg,R.M., van der Kooy,D. In vivo infusions of exogenous growth factors into the fourth ventricle of the adult mouse brain increase the proliferation of neural progenitors around the fourth ventricle and the central canal of the spinal cord. European Journal of Neuroscience 2002; 16(-6-):1045-1057. >>> Fibroblast growth factor 2; Epidermal growth factor; Heparin; Saline; Albumin, mouse; CSF/CNS (fourth ventricle); CSF/CNS; Mice; 6 days; Controls received mp w/ vehicle; peptides; EGF and FGF were infused alone or together, with or without heparin; heparin was added to prevent FGF degradation and to help present FGF to its receptor.

P5350 Bachoo,R.M., Maher,E.A., Ligon,K.L., Sharpless,N.E., Chan,S.S., You,M.J.J., Tang,Y., DeFrances,J., Stover,E., Weissleder,R., Rowitch,D.H., Louis,D.N., DePinho,R.A. Epidermal growth factor receptor and Ink4a/Arf: Convergent mechanisms governing terminal differentiation and transformation along the neural stem cell to astrocyte axis. CANCER CELL 2002; 1(-3-):269-277. >>> Epidermal growth factor; Saline; albumin, mouse serum; CSF/CNS; Mice; 1007D; 7 days; Peptides.

P5214 Permanne,B., Adessi,C., Saborio,G.P., Fraga,S., Frossard,M.J., Van Dorpe,J., Dewachter,I., Banks,W.A., Van Leuven,F., Soto,C. Reduction of amyloid load and cerebral damage in a transgenic mouse model of Alzheimer's disease by treatment with a beta-sheet breaker peptide. FASEB J 2002/6; 16(-8-):860-862. >>> Amyloid protein, beta; Saline; DMSO; CSF/CNS; Mice (Transgenic); 1002; 2 months; Controls received mp w/ vehicle; comparison of IP injections vs. icv infusion via mp; half-life (37 +/- 5 min); peptides: neurodegenerative (Alzheimer's disease); 10% DMSO in saline vehicle used.

P5066 Marsh,D.J., Weingarth,D.T., Novi,D.E., Chen,H.Y., Trumbauer,M.E. Melanin-concentrating hormone 1 receptor-deficient mice are lean, hyperactive, and hyperphagic and have altered metabolism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES O F AMERICA 2002; 99(-5-):3240-3245. >>> Melanin-concentrating hormone; CSF, artificial; CSF/CNS (third ventricle); mice; 6 days; controls received mp w/ vehicle; comparison of ICV injections vs mp; peptides; cannula patency verified via cresyl violet staining; mice received vehicle pumps for 6 days; pump model not indicated; pumps used had release rate of 0.5 ul/hr..

P5042 Pierroz,D.D., Ziotopoulou,M., Ungsunan,L., Moschos,S., Flier,J.S., Mantzoros,C.S. Effects of acute and chronic administration of the melanocortin agonist MTII in mice with diet-induced obesity. Diabetes 2002; 51(--):1337-1345. >>> MTII; PBS; CSF/CNS; mice; 1007D; 8 days; controls received mp w/ vehicle; comparison of acute and chronic ip injections vs. chronic administration via minipump; ALZET Brain Infusion Kit used; MTII is a melanocortin agonist.

P4928 Cooper,J.D., Salehi,A., Delcroix,J.D., Howe,C.L., Belichenko,P.V., Chua-Couzens,J., Kilbridge,J.F., Carlson,E.J., Epstein,C.J., Mobley,W.C. Failed retrograde transport of NGF in a mouse model of Down's syndrome : Reversal of cholinergic neurodegenerative phenotypes following NGF infusion. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2001; 98(-18-):10439-10444. >>> Nerve growth factor; CSF, artificial; CSF/CNS; mice; 1002; 2 weeks; controls received mp w/ vehicle; neurodegenerative (Alzheimer's disease); Down's Syndrome.

P4912 Hutcheson,D.M., Matthes,H.W.D., Valjent,E., Sanchez-Blazquez,P., Rodriguez-Diaz,M., Garzon,J., Kieffer,B.L., Maldonado,R. Lack of dependence and rewarding effects of deltorphin II in mu-opioid receptor-deficient mice. European Journal of Neuroscience 2001; 13(--):153-161. >>> Deltorphin II; CSF/CNS (third ventricle); mice; 2001; 6 days; controls received mp w/ vehicle; comparison of ICV injections vs. mp; no stres (see pg. 154); good methods (mice ICV implant; p. 154); 5-day recovery period; competitive injection was by Hamilton microsyringe; histological analysis confirmed cannula placementand lack of tissue damage;.

P4747 Kernie,S.G, Liebl,D.J, Parada,L.F  The European Molecular Biology Organization Journal 2000; 19(-6-):1290-1300. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-4/5;; PBS;; CSF/CNS (third ventricle);; mice;; 1002;; 14 days;; Controls received mp w/ vehicle; functionality of mp verified by residual volume after sacrifice; peptides; ALZET brain infusion kit used; brain cannula secured to skull by cyanoacrylate adhesive (Loctite 454); cannula patency verified by methlene blue injection after sacrifice; recomb. human BDNF used; all neurotrophins infused via same pump;.

P4735 Conover,J.C, Doetsch,F., Garcia-Verdugo,J.-M, Gale,N.W, Yancopoulos,G.D., Alvarez-Buylla,A. Disruption of Eph/ephrin signaling affects migration and proliferation in the adult subventricular zone.. Nature Neuroscience 2000; 3(-11-):1091-1097. >>> EphB2; Ephrin-B1; Ephrin-B2;; PBS;; CSF/CNS;; mice;; 1007D;; 3.5 days or 13 hours;; Functionality of mp verified by brain slice immunohistochemistry; immunology; peptides; EphB1-3 are receptor tyrosine kinases; Ephrins-B/2-3 are transmembrane ligants; Fusion proteins consisting of Fc domain of human IgG (immunoglobulin) infused for all peptide agents above..

P4659 Bradbury,M.J, McBurnie,M.I., Denton,D.A., Lee,K.-F, Vale,W.W. Modulation of urocortin-induced hypophagia and weight loss by corticotropin-releasing factor receptor 1 deficiency in mice.. Endocrinology 2000; 141(-8-):2715-2724. >>> Urocortin; CSF, artificial; CSF/CNS (third ventricle); Mice; 2001; 2002; 4 weeks; Comparison of ICV injections vs mp; pumps replaced 2 times; peptides; delayed delivery; 2002 aCSF pumps were replaced with 2001 hormone pumps; then, these were replaced for 2001 aCSF pumps;.

P4452 Li,M., Ona,V.O, Guégan,C, Chen,M, Jackson-Lewis,V, Andrews,L.J., Olszewski,A.J, Stieg,P.E, Lee,J.-P, Przedborski,S, Friedlander,R.M. Functional role of caspase-1 and caspase-3 in an ALS transgenic mouse model. Science 2000/4/14; 288(-14-):335-339. >>> zVAD-fmk; DMSO; Pipes; CSF/CNS; Mice (transgenic); 56 days; Controls received mp w/vehicle; pumps replaced after 28 days; zVAD-fmk is a broad caspase inhibitor; also called N-benzyloxycarbonyl-Val-Asp- fluoromethylketone; neurodegenerative (Amyotrophic Lateral Sclerosis).

P4369 Doetsch,F., Caille,I., Lim,D.A., Garcia-Verdugo,J.M., Alvarez-Buylla,A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 1999/6/11; 97(--):703-716. >>> Thymidine, [3H]-; Ara-C; Radio-isotopes; Saline; 3H Tracer; CSF/CNS; CSF/CNS (surface of brain); mice; 2002; 1007D; 6, 12 days; Ara-C is cytosine-b-D-arabinofuranoside; controls received mp with vehicle.

P4273 Cooper,J.D., Messer,A., Feng,A.K., Chua-Couzens,J., Mobley,W.C. Apparent loss and hypertrophy of interneurons in a mouse model of neuronal ceroid lipofuscinosis: evidence for partial response to insulin-like growth factor-1 treatment. Journal of Neuroscience 1999/4/1; 19(-7-):2556-2567. >>> Insulin-like growth factor I; CSF, artificial;; CSF/CNS;; mice; 7 days; controls received mp w/ vehicle; peptides;.

P4272 Chen,K.S., Nishimura,M.C., Armanini,M.P., Crowley,C., Spencer,S.D., Phillips,H.S. Disruption of a single allele of the nerve growth factor gene results in atrophy of basal forebrain cholinergic neurons and memory deficits. Journal of Neuroscience 1997/10/1; 17(-19-):7288-7296. >>> Nerve growth factor; CSF, artificial; serum, mouse; CSF/CNS; Mice; 1007D; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; peptides; ALZET brain infusion kit used; cannula secured to skull using cyanoacrylate adhesive; model 1007D pump was half coated w/ wax to reduce flow rate; human recomb. NGF used; ngf +/- mice used.

P4269 Halaas,J.L., Boozer,C., Blair-West,J., Fidahusein,N., Denton,D.A., Friedman,J.M. Physiological response to long-term peripheral and central leptin infusion in lean and obese mice. Proc.Natl.Acad.Sci.USA 1997/8; 94(--):8878-8883. >>> Leptin;; PBS; CSF, artificial;; SC; CSF/CNS (third ventricle);; mice; 30 days; controls received mp w/ vehicle; functionality of mp verified by plasma levels in SC infusion studies; peptides; recomb. murine leptin used; animals were infused with aCSF for one week before leptin or PBS was infused for 30 days, followed by 14 days of aCSF infusion; animals that did not respond to ICV leptin received ICV angiotensin II to confirm cannula placement..

P4237 Tsirka,S.E., Rogove,A.D., Bugge,T.H., Degen,J.L., Strickland,S. An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus. J.Neurosci. 1997; 17(-2-):543-552. >>> Antiplasmin, alpha 2-; CSF, artificial;; CSF/CNS; mice; 1007D; 7 days; controls received mp w/vehicle.

P3798 Halaas,J.L., Friedman,J.M. Leptin and its receptor. J Endocrinol 1997; 155(-2-):215-216. >>> Leptin; PBS; CSF/CNS (third ventricle); Mice; 26 days; Controls received w/ vehicle; pumps replaced after 14 days with ACSF containing pumps; peptides; 4-day surgical recovery period achieved by infusion of ACSF; 4-day ACSF infusion followed by 14 day Leptin infusion followed by 10 day ACSF infusion.

P3797 Ullman,M.D., Ventura,R.F., Draski,L.J., Deitrich,R.A., Baker,R.C. Effect of exogenous GM1 on ethanol sensitivity in selectively bred mouse lines. Alcohol Clin Exp Res 1997; 21(-4-):661-665. >>> GM1; Saline; 3H tracer; CSF/CNS; mice; 1003D; 72 hours; controls received mp w/saline; ALZET brain infusion kit used; cannula secured to skull using cyanoacrylate adhesive; brain tissue distribution.

P3256 Craig,C.G., Tropepe,V., Morshead,C.M., Reynolds,B.A., Weiss,S., van der Kooy,D. In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J.Neurosci. 1996; 16(-8-):2649-2658. >>> Epidermal growth factor; Transforming growth factor-a; Fibroblast growth factor; Nerve growth factor; Saline; Albumin, mouse serum; CSF/CNS; mice; 1007D; 6 days; controls received no pumps or mp w/vehicle; peptides; mouse EGF used; human recomb. basic FGF used; mouse 7S NGF used; human recomb. TGF-alpha used.

P3251 Campbell,A.D., Erwin,V.G. Changes in mouse brain neurotensin receptor density following chronic infusion of neurotensin. Peptides 1995; 16(-3-):501-504. >>> Neurotensin; Saline; CSF/CNS; mice; 3, 7 days; controls received mp w/saline; peptides; loop of tubing allowed flow cessation.