References on the Intracerebroventricular Administration of Agents to Mice Using ALZET® Osmotic Pumps

16330 Drougard, A., Fournel, A., Marlin, A., Meunier, E., Abot, A., Bautzova, T., Duparc, T., Louche, K., Batut, A., Lucas, A., Le-Gonidec, S., Lesage, J., Fioramonti, X., Moro, C., Valet, P., Cani, P. D., Knauf, C. Central chronic apelin infusion decreases energy expenditure and thermogenesis in mice. Sci Rep 2016; 6(--):31849. >>> Apelin; CSF, artificial; CSF/CNS (lateral ventricle); Mice; 2004; Controls received mp w/ vehicle; animal info (C57Bl6/J mice, 13-15 week old); animal info (C57Bl6/J mice, 13-15 week old);“stability of apelin and the duration of the treatment were compatible with the stability of the molecule” (pg. 8); peptides; anesthetised mice with isoflurane; Brain coordinates − 1 mm lateral, − 0.2 mm anteroposterior from the bregma and − 1.7 mm deep; Dose (20 nM);.

16305 Apkarian, A. V., Mutso, A. A., Centeno, M. V., Kan, L., Wu, M., Levinstein, M., Banisadr, G., Gobeske, K. T., Miller, R. J., Radulovic, J., Hen, R., Kessler, J. A. Role of adult hippocampal neurogenesis in persistent pain. Pain 2016; 157(-2-):418-28. >>> AraC; Saline; CSF/CNS (lateral ventricle); mice; 1002; Controls received mp w/ vehicle; animal info (C57Bl/6 male mice, 8-9 wks old); dose-response (pg 421); behavioral testing (touch and cold sensitivity of injured paw, Von Frey, Tail suspension test, Open field, Black-box emergence test);“Mice show fullbehavioral and functional recovery soon after surgery” pg 419; Brain coordinates; 0.4mmposterior to the bregma, 1mmlateral to the midline, and 2 mm ventral to the skull.

16301 Bhagat, S. M., Butler, S. S., Taylor, J. R., McEwen, B. S., Strittmatter, S. M. Erasure of fear memories is prevented by Nogo Receptor 1 in adulthood. Mol Psychiatry 2016; 21(-9-):1281-9. >>> Nogo Receptor (310) ecto-Fc; PBS; CSF/CNS (right lateral ventricle); mice; 28 days; Controls received mp w/ vehicle; animal info (Adult male C57BL6/J mice (4–6 mo old)); functionality of mp verified by behavioral endpoints; functionality of mp verified by behavioral endpoints; behavioral testing (fear conditioning, extinction training, extinction retrieval, fear reacquisition, cue selectivity); cyanoacrylate adhesive; NgR(310)ecto-Fc aka Nogo Receptor(310) ecto-Fc; Dose (0.2 mg); Resultant CNS protein levels;.

16287 de Kloet, A. D., Pitra, S., Wang, L., Hiller, H., Pioquinto, D. J., Smith, J. A., Sumners, C., Stern, J. E., Krause, E. G. Angiotensin Type-2 Receptors Influence the Activity of Vasopressin Neurons in the Paraventricular Nucleus of the Hypothalamus in Male Mice. Endocrinology 2016; 157(-8-):3167-80. >>> Compound 21; CSF, artificial; Saline; CSF/CNS (lateral ventricle); Mice, rats; Controls received mp w/ vehicle; animal info (Male mice (10–12 wk old) or rats (4–6 wk old)); functionality of mp verified by plasma vasopressin levels; ALZET brain infusion kit used; ALZET brain infusion kit used; Brain coordinates; from bregma 0.2 mm posterior, 1.0 mm lateral, and 2.5 mm ventral; Dose (7.5 ng/kg/hr);.

16267 Varvel, N. H., Grathwohl, S. A., Degenhardt, K., Resch, C., Bosch, A., Jucker, M., Neher, J. J. Replacement of brain-resident myeloid cells does not alter cerebral amyloid-beta deposition in mouse models of Alzheimer's disease. J Exp Med 2015; 212(-11-):1803-9. >>> Valganciclovir; PBS; CSF/CNS (ventricle); mice; 2004; Controls received mp w/ vehicle; animal info (Female hemizygous TK mice crossed w/ male hemizygous APPPS1 or hemizygous APP23); ALZET brain infusion kit 3 used; good methods (pg. 11807); post op. care (paracetamol, i.p. 5 mg/kg daily; acetaminophen i.p. 5 mg/kg daily); brain tissue distribution; Dental cement used; Cannula placement verified via staining; anesthetized using ketamine and xylazine; Brain coordinates; from bregma: +0.1 mm anteroposterior, 1.0 mm lateral, and 2.5 mm dorsoventral; Dose (50 mg/ml);.

16251 Sooy, K., Noble, J., McBride, A., Binnie, M., Yau, J. L., Seckl, J. R., Walker, B. R., Webster, S. P. Cognitive and Disease-Modifying Effects of 11beta-Hydroxysteroid Dehydrogenase Type 1 Inhibition in Male Tg2576 Mice, a Model of Alzheimer's Disease. Endocrinology 2015; 156(-12-):4592-603. >>> UE2316; DMSO, PEG; CSF, artificial; SC, CSF/CNS (ventricles); mice; 2004; Controls received mp w/ vehicle; animal info (Male C57BL/6 mice, 8-10 months, 24 month); functionality of mp verified ; 50:50 DMSO-PEG used; dose-response (p 4596); behavioral testing (memory in passive avoidance, Y Maze, Open field, spontaneous alternation, morris water maze); brain tissue distribution; Dose (5 mg/kg/d, 10 mg/kg/d, 15 mg/kg/d, 100 ng/h);.

16250 Smith, P. Y., Hernandez-Rapp, J., Jolivette, F., Lecours, C., Bisht, K., Goupil, C., Dorval, V., Parsi, S., Morin, F., Planel, E., Bennett, D. A., Fernandez-Gomez, F. J., Sergeant, N., Buee, L., Tremblay, M. E., Calon, F., Hebert, S. S. miR-132/212 deficiency impairs tau metabolism and promotes pathological aggregation in vivo. Hum Mol Genet 2015; 24(-23-):6721-35. >>> miR-132 mimics; In vivo transfection agent; CSF/CNS (ventricle); mice; 2006; Controls received mp w/ vehicle; animal info (Twelve-month-old 3xTg-AD WT mice); ALZET brain infusion kit 2 used; post op. care (50Ǩl Anafen (1 mg/ml) and 500Ǩl saline (0.9%)); behavioral testing (Barnes maze test); In vivo transfection agent used: jetPEI; Dose (1.8Ǩg/day); Brain coordinates; ventricle A/P =−0.22 M/l = 0.0 D/v = −3.5.

16236 Robins, S. C., Trudel, E., Rotondi, O., Liu, X., Djogo, T., Kryzskaya, D., Bourque, C. W., Kokoeva, M. V. Evidence for NG2-glia derived, adult-born functional neurons in the hypothalamus. PLoS One 2013; 8(-10-):e78236. >>> Uridine, bromodeoxy; uridine, ethynyldeoxy; CSF, artificial; CSF/CNS (right lateral ventricle); mice; 1007D; animal info: NG2-CreER:tdTomato mice; functionality of mp verified post brain removal; Plastics One infusion kit used; brain tissue distribution; delayed delivery (2 days); delayed delivery (2 days); Dose: 30 ug/day (BrdU and EdU); Brain coordinates; 0.3 mm (anteroposterior), 1.0 mm (lateral), and 2.5 mm (dorsoventral, below skull).

16233 Rabinovich-Toidman, P., Rabinovich-Nikitin, I., Ezra, A., Barbiro, B., Fogel, H., Slutsky, I., Solomon, B. Mutant SOD1 Increases APP Expression and Phosphorylation in Cellular and Animal Models of ALS. PLoS One 2015; 10(-11-):e0143420. >>> Antibody, monoclonal, BBS; CSF/CNS (right lateral ventricle); mice; 2006; Controls received mp w/ isotype-matched non relevant MAb; animal info (male Hemizygous B6SJLTgN (SOD1G93A) 1 Gur mice); brain infusion cannula used; neurodegenerative (ALS); immunology; antibodies infused; MAb aka Monoclonal antibody; Ketamine and Xylazine anesthesia; Dose (1.5 mg/mL); Brain coordinates; l-2mm [Bregma] in the anterio-posterior direction, 2.8mm in mediolateral direction and 3mm depth.

16232 Prokop, S., Miller, K. R., Drost, N., Handrick, S., Mathur, V., Luo, J., Wegner, A., Wyss-Coray, T., Heppner, F. L. Impact of peripheral myeloid cells on amyloid-beta pathology in Alzheimer's disease-like mice. J Exp Med 2015; 212(-11-):1811-8. >>> Ganciclovir; CSF, artificial; CSF/CNS (lateral ventricles); mice; 2001; Controls received mp w/ vehicle; animal info (APPPS1 transgenic mice); brain infusion cannula used; neurodegenerative (Alzheimer’s); Dose (2.5 mg/ml); Therapeutic indication (Alzheimer’s Disease);.

16230 Pikor, N. B., Astarita, J. L., Summers-Deluca, L., Galicia, G., Qu, J., Ward, L. A., Armstrong, S., Dominguez, C. X., Malhotra, D., Heiden, B., Kay, R., Castanov, V., Touil, H., Boon, L., O'Connor, P., Bar-Or, A., Prat, A., Ramaglia, V., Ludwin, S., Turley, S. J., Gommerman, J. L. Integration of Th17- and Lymphotoxin-Derived Signals Initiates Meningeal-Resident Stromal Cell Remodeling to Propagate Neuroinflammation. Immunity 2015; 43(-6-):1160-73. >>> Anti-interleukin 22, anti-interleukin 17, LymphotoxinǞR-Ig; Immunoglobulin; CSF/CNS (cerebroventricular); mice; 2004; Controls received mp w/ vehicle; animal info (SJL/J mice,); animal info (SJL/J mice,); dose-response (pg 1166); peptides; Fibroblast remodeling in vivo; Dose (2 mg/ml (LTBR), 2.25 mg/ml (IL));.

16228 Pazos, P., Lima, L., Tovar, S., Gonzalez-Touceda, D., Dieguez, C., Garcia, M. C. Divergent responses to thermogenic stimuli in BAT and subcutaneous adipose tissue from interleukin 18 and interleukin 18 receptor 1-deficient mice. Sci Rep 2015; 5(--):17977. >>> Interleukin-18; Saline; CSF/CNS (lateral ventricle); mice; 1007D; Controls received mp w/ vehicle; animal info (10–11 weeks old Il18r1-KO, WT mice); brain infusion kit used; behavioral testing (cold-challenge); brain tissue distribution; effects of interleukin-18 on food intake, body composition and iWAT morphology; Dose (20Ǩg/mouse/7 days,);.

16226 Parsi, S., Smith, P. Y., Goupil, C., Dorval, V., Hebert, S. S. Preclinical Evaluation of miR-15/107 Family Members as Multifactorial Drug Targets for Alzheimer's Disease. Mol Ther Nucleic Acids 2015; 4(--):e256. >>> miR-16 mimic; Saline; CSF/CNS (ventricle); mice; 1007D; Controls received mp w/ vehicle; animal info (Wild-type mice (C57BL/6, female, 2 months old)); ALZET brain infusion kit 2 used; dose-response (pg. 2); neurodegenerative (Alzheimer’s); post op. care (Anafen); replacement therapy (miRNA replacement therapy); brain tissue distribution; delayed delivery (12 hours delivery/day); potent inhibitor of amyloid precursor protein (APP) and BACE1 expression, AǞpeptide production, and Tau phosphorylation in cells; Dose (4.2Ǩg/Ǩl); Brain coordinates; ventricle A/P =−0.22 M/L = 0.5 D/v = 3.5).

16222 Ono, D., Honma, S., Honma, K. Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms. Eur J Neurosci 2015; 42(-12-):3128-37. >>> D-Luciferin K; Saline, physiological; CSF, artificial; IP, CSF/CNS (lateral ventricle); mice; 2002, 1004; Controls received mp w/ vehicle; animal info (Male and female mice, C57BL/6j, 3.5-7 months); functionality of mp verified by measurement of bioluminescence; functionality of mp verified by measurement of bioluminescence; Isoflurane anesthesia; circadian rhythm measurements; Dose (100 mM);.

16254 Yongzhi Li,, Keiko Tanaka,, Li Wang,, Yasuhito Ishigaki, Nobuo Kato4  Journal of Experimental Medicine 2015; 237(--):329-338. >>> NMDAR-CSF; saline; CSF/CNS (left lateral ventricle); mice; 1004; Controls received mp w/ vehicle; animal info: C57BL/6 male mice (age: 8 weeks; weight: 22-24 g); functionality of mp verified by behavioral tests; neurodegenerative (anti-NMDAR encephalitis); behavioral testing (spontaneous locomotor activity, open field test, NOR test, Morris Water maze); Immunology, immunotherapy; brain tissue distribution; cyanoacrylate adhesive;“The diffusion of methlene blue in all ventricles was confirmed in all mice examined” pg 331; Cannula placement verified via methylene blue or thionine solution injection into brain; NMDAR-CSF aka Anti-N-methyl-D-aspartate receptor-CSF; CSF collected from patients with anti-NMDAR encephalitis; Brain coordinates; 0.4 mm posterior to bregma, 1.0 mm lateral to the lambda point, and 2.0 mm deep from the dural surface.

16193 Kim, J. S., Brownjohn, P. W., Dyer, B. S., Beltramo, M., Walker, C. S., Hay, D. L., Painter, G. F., Tyndall, J. D., Anderson, G. M. Anxiogenic and Stressor Effects of the Hypothalamic Neuropeptide RFRP-3 Are Overcome by the NPFFR Antagonist GJ14. Endocrinology 2015; 156(-11-):4152-62. >>> Peptide-3, RF amide-related; GJ14; CSF/CNS (left lateral ventricle); mice; 1007D; Controls received mp w/ vehicle; animal info: Female C57BL/6 mice (8 wk old); functionality of mp verified by behavior testing; behavioral testing (elevated plus maze and open field tests for 5 minutes each); peptides; RFRP-3 aka Peptide-3, RF amide-related; GJ14 is a NPFF1R antagonist with moderate antagonism at NPFF2R; G-protein coupled neuropeptide FF (NPFF) receptors belong to the RFamide receptor family and are referred to as NPFF1R (also known as GPR147) and NPFF2R (GPR74); Dose: 2 nmol/mouse/d (RFRP-3); 20 nmol/mouse/d (GJ14).

16192 Kim, J., Yoon, H., Horie, T., Burchett, J. M., Restivo, J. L., Rotllan, N., Ramirez, C. M., Verghese, P. B., Ihara, M., Hoe, H. S., Esau, C., Fernandez-Hernando, C., Holtzman, D. M., Cirrito, J. R., Ono, K., Kim, J. microRNA-33 Regulates ApoE Lipidation and Amyloid-beta Metabolism in the Brain. J Neurosci 2015; 35(-44-):14717-26. >>> anti-miR-33; CSF/CNS (intracerevrovenctricular); Mice; Controls received negative control; animal info: mir-33 knock-out mice, two month-old APPsw/PSEN1_9 (APP/PS1) transgenic mice; neurodegenerative (Alzheimer’s); immunology; brain tissue distribution; Dose: 30 ug/d.

16185 Su, C. H., Tsai, C. Y., Chang, A. Y., Chan, J. Y., Chan, S. H. MRI/DTI of the Brain Stem Reveals Reversible and Irreversible Disruption of the Baroreflex Neural Circuits: Clinical Implications. Theranostics 2016; 6(-6-):837-48. >>> Angiotensin II; CSF/CNS (ventricle); Mice; 1007D; animal info (male, C57BL6, adult); cardiovascular; peptides; bp measured using radiotelemetry (DSI); Dose (7.5 ug/hr);.

16132 Hamilton, L. K., Dufresne, M., Joppe, S. E., Petryszyn, S., Aumont, A., Calon, F., Barnabe-Heider, F., Furtos, A., Parent, M., Chaurand, P., Fernandes, K. J. Aberrant Lipid Metabolism in the Forebrain Niche Suppresses Adult Neural Stem Cell Proliferation in an Animal Model of Alzheimer's Disease. Cell Stem Cell 2015; 17(-4-):397-411. >>> Ara-C; Oleic Acid; AB142089; BSA; CSF/CNS (cerebroventricular); mice; 1007D; 1004; Controls received mp w/ vehicle; animal info: 2-month-old WT or 3xTg-AD mice; functionality of mp verified by Analysis of brain sections by IMS; cancer; dose-response (pg 407); neurodegenerative (NSC impairment); enzyme inhibitor (SCD-1 inhibitor– AB142089); Dose: 500 mM Oleic Acid; 10mM C12 Oleic Acid conjugated to vehicle; 10mM C13 Oleic Acid conjugated to vehicle; 2% AraC; 40 mM SCD-1 inhibitor.

16128 Giachino, C., Boulay, J. L., Ivanek, R., Alvarado, A., Tostado, C., Lugert, S., Tchorz, J., Coban, M., Mariani, L., Bettler, B., Lathia, J., Frank, S., Pfister, S., Kool, M., Taylor, V. A Tumor Suppressor Function for Notch Signaling in Forebrain Tumor Subtypes. Cancer Cell 2015; 28(-6-):730-42. >>> Platelet-derived growth factor - A, human recombinant; CSF/CNS (forebrain lateral ventricle); mice; animal info: adult Hes5::GFP Notch-reporter mice; cancer (gliomas); brain tissue distribution;“increased PDGF signaling induces glioma-like growths from the subventricular zone (SVZ) in mice” (pg 731);.

16127 Ghanbari, A., Esmaeilpour, T., Bahmanpour, S., Golmohammadi, M. G., Sharififar, S., Azari, H. Depletion of neural stem cells from the subventricular zone of adult mouse brain using cytosine b-Arabinofuranoside. Brain Behav 2015; 5(-11-):e00404. >>> Ara-C; Saline; CSF/CNS (right lateral ventricle); Mice; 1007D; Controls received sham operation and mp w/ vehicle; animal info: Five-week-old male C57-BL6 mice; pumps replaced once after 7 days; ALZET brain infusion kit 3 used; good methods (pg 2); post op. care (0.5 mg/kg buprenorphine every 12 h for 48 hours); cyanoacrylate adhesive (Loctite 454); delayed delivery (6-12 hours); Dose: 2% Ara-C in Saline; Brain coordinates; Paxinos mouse brain Atlas: Anterior-Posterior (AP): _0. 2, Medial-Lateral (ML): +0. 9 from the bregma point and Dorsal-Ventral: +2.7 from the top of skull.

16120 Ferrón, Sacri R., Andreu-Agulló, Celia, Mira, Helena, Sánchez, Pilar, Ángeles Marqués-Torrejón, M., Fariñas, Isabel  Nature Protocols 2007; 2(-4-):849-859. >>> Growth Factor; Saline; CSF/CNS (ventricle); mice; 1007D; animal info: any; ALZET brain infusion kit 3 used; good methods (pg 854); post op. care (mice placed on thermal blanket); brain tissue distribution; Cannula placement verified via tissue distribution upon brain removal; Brain coordinates; 0.0 antero-posterior, 0.7 medio-lateral stereotaxic coordinates.

16113 Dorr, A., Kress, E., Podschun, R., Pufe, T., Tauber, S. C., Brandenburg, L. O. Intrathecal application of the antimicrobial peptide CRAMP reduced mortality and neuroinflammation in an experimental model of pneumococcal meningitis. J Infect 2015; 71(-2-):188-99. >>> Antimicrobial peptide, cathelicidin-related; Saline; CSF/CNS (right lateral ventricle); mice; 1007D; Controls received mp w/ saline; animal info: male C57BL/6 mice (weight 19-23 g, aged 2-3 months); ALZET brain infusion kit 2 used; good methods (pg 189-190); brain tissue distribution of CRAMP; peptides; CRAMP aka Cathelicidin-related antimicrobial peptide; Dose: 12 ug/d.

16014 Jackson, K. L., Palma-Rigo, K., Nguyen-Huu, T. P., Davern, P. J., Head, G. A. Actions of rilmenidine on neurogenic hypertension in BPH/2J genetically hypertensive mice. J Hypertens 2014; 32(-3-):575-86. >>> rilmenidine; Ringer’s solution; CSF/CNS (ventricles); mice; 1002; Controls received mp w/ ringer’s solution vehicle; animal info (BPH/2J and BPN/3J mice); functionality of mp verified by behavioral tests; dose-response (pg 577-582, description and graphical representation); behavioral testing (pg 577; mice were exposed to aversive behavioral stimuli; restraint stress and dirty cage-switch tests conducted); tissue perfusion (brain tissue); antihypertensive; antihypertensive; Dose: 15ug/hr of rilmenidine.

16012 Ghadge, G. D., Pavlovic, J. D., Koduvayur, S. P., Kay, B. K., Roos, R. P. Single chain variable fragment antibodies block aggregation and toxicity induced by familial ALS-linked mutant forms of SOD1. Neurobiol Dis 2013; 56(--):74-8. >>> antibody, monoclonal anti-SOD1; CSF/CNS (ventricles); mice; animal info: FALS transgenic mice; anti SOD1 monoclonal antibody; neurodegenerative (Amyotrophic Lateral Sclerosis); paper does not mention ALZET much, or pump model;.

15973 Yael Kusne,, Eugenio A. Carrera-Silva,, Anthony S. Perry,, Elisabeth J. Rushing,, Edward K. Mandell,, Justin D. Dietrich,, Andrea E. Errasti,, Daniel Gibbs,, Michael E. Berens,, Joseph C. Loftus,, Christopher Hulme,, Weiwei Yang,, Zhimin Lu,, Kenneth Aldape,, Nader Sanai,, Carla V. Rothlin,, Sourav Ghosh  Science Signaling 2015; 7(-338-):1-15. >>> PZ09; CSF/CNS (ICV); mice; animal info (Six- to 8-week-old female NOD-SCID, TNFa−/−, and control mice); dose-response (pg. 2-5); tissue perfusion (brain; gliobastomas); PZ09 aka small-molecule, benzimidazoleadenosine triphosphate–competitive aPKC inhibitor; xenografts; Dose: 10 uM PZ09.

15916 Kim, D. H., Moon, E. Y., Yi, J. H., Lee, H. E., Park, S. J., Ryu, Y. K., Kim, H. C., Lee, S., Ryu, J. H. Peptide fragment of thymosin beta4 increases hippocampal neurogenesis and facilitates spatial memory. Neuroscience 2015; 310(--):51-62. >>> Ac-SDKP; PBS; CSF/CNS (third ventricle); Mice; 1007D; animal info (male, C57BL6J, 25-28g, 8 weeks old); behavioral testing (Morris water maze); tissue perfusion (third ventricle); peptides; Cannula placement verified via histologic analysis; used Plastics one cannula; brain coordinates;.

15898 Yanamandra, K., Kfoury, N., Jiang, H., Mahan, T. E., Ma, S., Maloney, S. E., Wozniak, D. F., Diamond, M. I., Holtzman, D. M. Anti-tau antibodies that block tau aggregate seeding in vitro markedly decrease pathology and improve cognition in vivo. Neuron 2013; 80(-2-):402-14. >>> Antibodies, anti-tau; PBS; CSF/CNS (ICV); mice; 2006; long-term study; controls received mp w/ vehicle anti-AB HJ3.4 and PBS; P301S, tau pathology, 5 months old; pumps replaced every 6 weeks ; neurodegenerative (tau pathology);.

15713 Ghanashyam D. Ghadge, John D. Pavlovic,, Sujatha P. Koduvayur,, Brian K. Kay,, Raymond P. Roos  NEUROBIOLOGY OF DISEASE 2013; 56(--):74-78. >>> anti-SOD1 monoclonal antibodies (mAbs); CSF/CNS (intraventricular); mice; not specified; FALS transgenic mice; anti SOD1 monoclonal antibody; neurodegenerative (Amyotrophic Lateral Sclerosis); paper does not mention ALZET much, or pump model..

15276 Jianfang Chen,, Eileen Marks, Barry Lai,, Zhaojie Zhang,, James A. Duce,, Linh Q. Lam,, Irene Volitakis,, Ashley I. Bush,, Steven Hersch,, Jonathan H. Fox  PLoS One 2013; 8(-10-):12. >>> deferoxamine; CSF/CNS (left lateral ventricle); mice; 1002; 6 wk old, R6/2 HD mice; neurodegenerative (Huntington's disease); dose: 1 nmole/hr.

15121 Trivedi, C., Shan, X.Y., Tung, Y.C.L., Kabra, D., Holland, J., Amburgy, S., Heppner, K., Kirchner, H., Yeo, G.S.H., Perez-Tilve, D., Perez-Tilve, D. Tachykinin-1 in the Central Nervous System Regulates Adiposity in Rodents. ENDOCRINOLOGY 2015; 156(--):1714-1723. >>> Ghrelin; neuropeptide K; Saline; CSF/CNS (fourth ventricle); CSF/CNS; Rat; mice; 1002;1007D; Controls received mp w/ vehicle; animal info (rat male, Wistar, 260-290g; mice male, C57Bl6, 12-16 weeks old); ALZET brain infusion kit used; dose-response (pg 1718); post op. care (analgesics); behavioral testing (locomotor activity); neuropeptide K aka NPK;.

15089 Stark, R., Reichenbach, A., Lockie, S.H., Pracht, C., Wu, Q.L., Tups, A., Andrews, Z.B., Andrews, Z.B. Acyl Ghrelin Acts in the Brain to Control Liver Function and Peripheral Glucose Homeostasis in Male Mice. ENDOCRINOLOGY 2015; 156(--):858-868. >>> Ghrelin, acyl; CSF, artificial; CSF/CNS (third ventricle); Mice; 1007D; Controls received mp w/ vehicle; animal info (male, C57BL6); ALZET brain infusion kit 3 used; tissue perfusion (third ventricle);.

15056 Pereira, L., Medina, R., Baena, M., Planas, A.M., Pozas, E., Pozas, E. IFN gamma regulates proliferation and neuronal differentiation by STAT1 in adult SVZ niche. Frontiers in Cellular Neuroscience 2015; 9(--):U1-U10. >>> Interferon, gamma; Saline; CSF/CNS (third ventricle); Mice; 1007D; Controls received mp w/ vehicle; animal info (male, STAT2 KO or 129S6/SvEv); immunology;.

14485 Wu, Y.W., Tu, Q.S., Valverde, P., Zhang, J., Murray, D., Dong, L.Q., Cheng, J., Jiang, H., Rios, M., Morgan, E., Tang, Z.H., Chen, J.K., Chen, J.K. Central adiponectin administration reveals new regulatory mechanisms of bone metabolism in mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2014; 306(--):E1418-E1430. >>> Adiponectin, globular; adiponectin, full-length; CSF, artificial; CSF/CNS (third ventricle); Mice; Controls received mp w/ vehicle; animal info (male, APN-KO or WT, 20-25g, 4-6 weeks old); ALZET brain infusion kit 2 used; tissue perfusion (third ventricle); cyanoacrylate adhesive; Cannula placement verified via micrograph imaging; used tygon tubing to attach BIK cannula to pump;.

14429 Tan, M.S., Tan, L., Jiang, T., Zhu, X.C., Wang, H.F., Jia, C.D., Yu, J.T., Tan, L. Amyloid-beta induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer's disease. Cell Death&Disease 2014; 5(--):U228-U239. >>> RNA, small interfering NLRP1; RNA, small interfering, NLRP3; RNA, small interfering caspase-1; CSF, artificial; water, RNase-free; CSF/CNS (dorsal third ventricle); Mice (transgenic); Controls received mp w/ vehicle or control RNA; animal info (male, APP/PS1); functionality of mp verified by knowckdown of targetd gene; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); no stress"This dose of NLRP1 siRNA, caspase-1 siRNA or NLRP3 siRNA infusion was well tolerated, and no signs of neurotoxicity including hind-limb paralysis, vocalization, food intake, or neuroanatomical damage were observed in preliminary study."(see pg. 10); behavioral testing (morris water maze); tissue perfusion (dorsal third ventricle);"Our study using this approach of pump-mediated siRNA infusion is efficient in downregulation of NLRP1 mRNA (by about 60%) and protein (by about 50%) levels in APP/PS1 brain. And the treatment with control siRNA did not alter cerebral NLRP1 mRNA and protein levels compared with No siRNA-treated APP/PS1 mice, excluding an effect of pump-mediated infusion on NLRP1 expression levels."pg 8;.

13821 Jiang, T., Yu, J.T., Zhu, X.C., Tan, M.S., Gu, L.Z., Zhang, Y.D., Tan, L., Tan, L. Triggering receptor expressed on myeloid cells 2 knockdown exacerbates aging-related neuroinflammation and cognitive deficiency in senescence-accelerated mouse prone 8 mice. NEUROBIOLOGY OF AGING 2014; 35(--):1243-1251. >>> RNA, small interfering TREM2; Cerebrospinal fluid, artifical; CSF/CNS (dorsal third ventricle); Mice; 1004; Controls received mp w/ vehicle or control siRNA; animal info (SAMP8, 6 months old); functionality of mp verified by decrease TREM2 protein levels pg. 1246; ALZET brain infusion kit used; neurodegenerative (Alzheimers); no stress (see pg. 1244); behavioral testing (Morris water maze); tissue perfusion (third ventricle); immunology;.

13711 Azzi, A., Dallmann, R., Casserly, A., Rehrauer, H., Patrignani, A., Maier, B., Kramer, A., Brown, S.A. Circadian behavior is light-reprogrammed by plastic DNA methylation. Nature Neuroscience 2014; 17(-3-):U62-U69. >>> Zebularine; Cerebrospinal fluid, artificial; DMSO; CSF/CNS (third ventricle); Mice; 1004; Controls received mp w/ vehicle; animal info (C57BL6J, 6 weeks old); behavioral testing (wheel-running behavior);"To ensure that we could reliably chronically infuse zebularine into the third ventricle in a way that pharmacologically influences the SCN, we performed a control experiment in which mice were chronically infused with TGFa for 7 d using ALZET osmotic mini pump model 1007D as described"Online methods pg1; Cannula placement verified via visual inspection; Zebularine is a cytidine analog and DNA methyltransferase inhibitor; circadian rhythm study;.

13669 Wu, Q., Zheng, R.M., Srisai, D., McKnight, G.S., Palmiter, R.D. NR2B subunit of the NMDA glutamate receptor regulates appetite in the parabrachial nucleus. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2013; 110(-36-):14765-14770. >>> Lithium chloride; RO25-6981; bretazenil; Saline; CSF/CNS (fourth ventricle; parabrachial nucleus); Mice; 1002; Controls received mp w/ vehicle or sham surgery; animal info (male, Agrp DTR/+ or WT); bilateral cannula used; dose-response (pg.14766, fig.3; pg.14769, fig.6); behavioral testing (food intake); tissue perfusion (fourth ventricle; parabrachial nucleus); Cannula placement verified; RO25-6981 is a NR2B antagonist; bilateral infusion;.

13660 Tan, M.S., Yu, J.T., Jiang, T., Zhu, X.C., Guan, H.S., Tan, L. IL12/23 p40 Inhibition Ameliorates Alzheimer's Disease-Associated Neuropathology and Spatial Memory in SAMP8 Mice. JOURNAL OF ALZHEIMERS DISEASE 2014; 38(-3-):633-646. >>> RNA, small interfering p40; Cerebrospinal fluid, artificial; CSF/CNS (third ventricle); Mice; 1004; Controls received mp w/ control siRNA, aCSF, control antibody; animal info (male, SAMP8, SAMR1, 3 month, 7 month and 11 month old); functionality of mp verified by increased expression of IL-12 and IL-23; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer); behavioral testing (Morris water maze);.

13655 Pandey, N.R., Zhou, X., Qin, Z.H., Zaman, T., Gomez-Smith, M., Keyhanian, K., Anisman, H., Brunel, J.M., Stewart, A.F.R., Chen, H.H. The LIM Domain Only 4 Protein Is a Metabolic Responsive Inhibitor of Protein Tyrosine Phosphatase 1B That Controls Hypothalamic Leptin Signaling. Journal of Neuroscience 2013; 33(-31-):12647-12655. >>> Leptin; Saline; CSF/CNS (third ventricle); Mice; Animal info (male, LMO4KO); behavioral testing (paired vs single feeding); diabetes; used guide cannula from Plastics One. used dental cement/screws.

13642 Howell, K.K., Monk, B.R., Carmack, S.A., Mrowczynski, O.D., Clark, R.E., Anagnostaras, S.G. Inhibition of PKC disrupts addiction-related memory. Frontiers in Behavioral Neuroscience 2014; 8(-;-):U1-U9. >>> Peptide, zeta-inhibitory; chelerythrine; Cerebrospinal fluid, artificial; PBS; CSF/CNS (third ventricle); Mice; 1002; Controls received mp w/ vehicle; animal info (C57BL/6Jx129T2SvEms/J, adult); ALZET brain infusion kit 3 used; post op. care (buprenorphine injection); behavioral testing (locomotion, locomotor sensitization); pulsatile delivery; dependence; peptides; zeta-inhibitory peptide aka ZIP; Lynch coil of ZIP administration for 14.5 hours; used mineral oil; Schematic of pump implantation pg.3 Fig 1A;.

12979 Arteaga-Solis, E., Zee, T., Emala, C.W., Vinson, C., Wess, J., Karsenty, G. Inhibition of Leptin Regulation of Parasympathetic Signaling as a Cause of Extreme Body Weight-Associated Asthma. Cell Metabolism 2013; 17(-1-):35-48. >>> Leptin; CSF/CNS (third ventricle); Mice; Control animals received mp w/ PBS; animal info (DIO-WT, 5 wks old); ALZET brain infusion kit 2 used; infusion rate of 0.5 ul/hr.

12688 Malkesman, O., Austin, D.R., Tragon, T., Henter, I.D., Reed, J.C., Pellecchia, M., Chen, G., Manji, H.K. Targeting the BH3-interacting domain death agonist to develop mechanistically unique antidepressants. Molecular Psychiatry 2012; 17(-8-):770-780. >>> Citalopram; BI-2A7; BI-11A7; L447951; CSF/CNS (third ventricle); Mice; 1002; Control animals received mp w/ saline; animal info (129S1/SVImJ, 20-25 g, male, 8 wks old); brain infusion kit 3 used.

12455 Kazanis, I., Ffrench-Constant, C. The Number of Stem Cells in the Subependymal Zone of the Adult Rodent Brain is Correlated with the Number of Ependymal Cells and Not with the Volume of the Niche. STEM CELLS AND DEVELOPMENT 2012; 21(-7-):1090-1096. >>> Ara-C; CSF/CNS (subventricular); Rat; mice; 1007D; Controls received mp w/ saline; animal info (Sprague Dawley, 2 mo old, adult, 129sv); ALZET brain infusion kit II used.

12071 Wu, Q., Clark, M.S., Palmiter, R.D. Deciphering a neuronal circuit that mediates appetite. Nature 2012; 483(-7391-):594-U112. >>> Ondansetron; CSF/CNS (fourth ventricle); SC; Mice; 1002; Animal info (Agrp Dtr/+, wt); Plastics One cannula used; cannula placement verified at the end of the experiment; ondansetron is an anti-nausea drug.

11520 Musto, A.E., Gjorstrup, P., Bazan, N.G. The omega-3 fatty acid-derived neuroprotectin D1 limits hippocampal hyperexcitability and seizure susceptibility in kindling epileptogenesis. Epilepsia 2011; 52(-9-):1601-1608. >>> Docosahexaenoic acid, deuterium labeled; neuroprotectin D1; Albumin, human serum; CSF/CNS (third ventricle); SC; Mice; 1007D; Controls received mp w/ vehicle; animal info (adult, male, C57BL/6, 25-30 g); pumps replaced after 1 week.

11483 Liu, Q.A., Zhang, J.A., Zerbinatti, C., Zhan, Y., Kolber, B.J., Herz, J., Muglia, L.J., Bu, G.J. Lipoprotein Receptor LRP1 Regulates Leptin Signaling and Energy Homeostasis in the Adult Central Nervous System. PLOS BIOLOGY 2011; 9(-1-):U115-U123. >>> Leptin; CSF, artificial; CSF/CNS (third ventricle); Mice; Controls received mp w/ vehicle; animal info (12 months old, LRP1-KO, wt); pump connected to catheter after one week recovery period.

11475 Li, X.S., Wu, X.P., Camacho, R., Schwartz, G.J., LeRoith, D. Intracerebroventricular Leptin Infusion Improves Glucose Homeostasis in Lean Type 2 Diabetic MKR Mice via Hepatic Vagal and Non-Vagal Mechanisms. PLoS One 2011; 6(-2-):U261-U267. >>> Leptin; CSF, artificial; CSF/CNS (third ventricle); Mice; 1004; Controls received mp w/ vehicle; animal info (MRK type 2 diabetes, male); ALZET brain infusion kit used; Plastics One cannula used; polyethylene catheter tubing used; pump connected to catheter after 1 week recovery period; cannula placement verified post mortem.

 

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What researchers are saying...

“In summary, our data demonstrate that the local microinfusion of endostatin is able to inhibit tumor growth and increase survival of animals with orthotopic malignant gliomas. Local continuous i.c. delivery is more effective than daily systemic delivery.” (p.1255) Schmidt et al., Clinical Cancer Research 2004;10:1255-1262.