Video Article Demonstrating ICV Delivery to the Mouse CNS

DeVos SL and Miller TM. Direct intraventricular delivery of drugs to the rodent central nervous system. J Vis Exp. 2013 May 12;(75):e50326. doi:10.3791/50326.

 

 

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This video article (JoVE) describes a method for targeted administration of drugs to the central nervous system by either continuous delivery using ALZET Osmotic Pumps or performing bolus injections into the right lateral ventricle of mice. Researchers at Washington University in St. Louis School of Medicine focused specifically on the delivery of antisense oligonucleotides to mice. However, this technique is readily adaptable to other drugs and other rodent species (i.e., rats).

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Pump Advantages

  • Ensure around-the-clock exposure to test agents at predictable levels
  • Permit continuous administration of short half-life proteins and peptides
  • Provide a convenient method for the chronic dosing of laboratory animals
  • Minimize unwanted experimental variables and ensure reproducible, consistent results
  • Eliminate the need for nighttime or weekend dosing
  • Reduce handling and stress to laboratory animals
  • Small enough for use in mice or very young rats
  • Allow for targeted delivery of agents to virtually any tissue
  • Cost-effective research tool