References on Immunology Research Using ALZET^® Osmotic Pumps

Q0479 Dang,M.H., Kato,H., Ueshiba,H., Omori-Miyake,M., Yamagoe,S., Ando,K., Imanishi,K., Arimura,Y., Haruta,I., Kotani,T., Ozaki,M., Suzuki,K., Uchiyama,T., Yagi,J. Possible role of LECT2 as an intrinsic regulatory factor in SEA-induced toxicity in D-galactosamine-sensitized mice. CLINICAL IMMUNOLOGY 2010; 137(-3-):311-321. >>> Antigen, staphylococcal enterotoxin A; SC; Mice; 2001; ; Animal info (B6, LECT2-/-, female, 8-12 wks old); post op. care (penicillin); immunology.

Q0583 Milner,J.D., Orekov,T., Ward,J.M., Cheng,L., Torres-Velez,F., Junttila,I., Sun,G.P., Buller,M., Morris,S.C., Finkelman,F.D., Paul,W.E. Sustained IL-4 exposure leads to a novel pathway for hemophagocytosis, inflammation, and tissue macrophage accumulation. Blood 2010; 116(-14-):2476-2483. >>> Interleukin-4, recomb. mouse; interleukin-13 recomb. mouse; SC; Mice; 3 days; Controls received mp w/ PBS; animal info (C57BL6, b6 Rag2 -/-, b6 Stat6 -/-); 100 ul sized pump used; immunology.

Q0336 Park,H.B., Oh,K., Garmaa,N., Seo,M.W., Byoun,O.J., Lee,H.Y., Lee,D.S. CP-690550, a Janus Kinase Inhibitor, Suppresses CD4+ T-Cell-Mediated Acute Graft-Versus-Host Disease by Inhibiting the Interferon-gamma Pathway. Transplantation 2010; 90(-8-):825-835. >>> CP690550; Mice; 14 days; Animal info ((B6xbm12)F1 (H-2bxbm12)); immunology; enzyme inhibitor (janus kinase, JAK3).

P9932 Park,J.H., Adoro,S., Guinter,T., Erman,B., Alag,A.S., Catalfamo,M., Kimura,M.Y., Cui,Y.Z., Lucas,P.J., Gress,R.E., Kubo,M., Hennighausen,L., Feigenbaum,L., Singer,A. Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells. NATURE IMMUNOLOGY 2010; 11(-3-):257-U10. >>> Interleukin-7, recomb, mouse; SC; Mice; 2 weeks; Animal info (79Z); immunology.

Q0599 Smith,M.S., Goldman,D.C., Bailey,A.S., Pfaffle,D.L., Kreklywich,C.N., Spencer,D.B., Othieno,F.A., Streblow,D.N., Garcia,J.V., Fleming,W.H., Nelson,J.A. Granulocyte-Colony Stimulating Factor Reactivates Human Cytomegalovirus in a Latently Infected Humanized Mouse Model. Cell Host&Microbe 2010; 8(-3-):284-291. >>> Granulocyte-Colony Stimulating Factor; AMD3100; SC; Mice; 1007D; 7 days; Controls received mp w/ PBS; animal info (7-10 wks old, NOD-scid, IL2Rgc null); immunology.

P9401 Cook,C.H., Bickerstaff,A.A., Wang,J.J., Zimmerman,P.D., Forster,M.R., Nadasdy,T., Colvin,R.B., Hadley,G.A., Orosz,C.G. Disruption of Murine Cardiac Allograft Acceptance by Latent Cytomegalovirus. AMERICAN JOURNAL OF TRANSPLANTATION 2009; 9(-1-):42-53. >>> Gallium nitrate; SC; Mice; 2002; 28 days; Controls received mp w/ agent and no transplantation; no stress (see pg. 44); cadiovascular; immunology; animal info (C57BL/6); heterotropic cardiac transplantation.

P9410 Hasegawa,T., Iwanaga,K., Hultquist,D.E., Liao,H., Visovatti,S.H., Pinsky,D.J. Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-4-):H1007-H1016. >>> Riboflavin; Saline; IP; Mice; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by plasma riboflavin levels; dose-response (fig. 1); cardiovascular; immunology; animal info (male, CBA/J, B1OA, 8-12 wks old, cardiac transplantation); antioxidant.

P9512 Hinojosa,E., Boyd,A.R., Orihuela,C.J. Age-Associated Inflammation and Toll-Like Receptor Dysfunction Prime the Lungs for Pneumococcal Pneumonia. Journal of Infectious Diseases 2009; 200(-5-):546-554. >>> Tumor necrosis factor-a; PBS; albumin, mouse serum; SC; Mice; 1007D; 5, 6 days; Controls received mp w/ vehicle; animal info (young Balb/cby, adult); immunology.

R0252 Jacob,A., Wu,R.Q., Zhou,M., Wang,P. Mechanism of the Anti-inflammatory Effect of Curcumin: PPAR-gamma Activation. PPAR Research 2009; 2007(-89369-):-. >>> Curcumin; IV; Rat; 2ML1; 3 days; Immunology; phytochemical component in turmeric; antioxidant properties; anti-inflammatory.

Q0809 Kim,S., Shen,T., Min,B. Basophils Can Directly Present or Cross-Present Antigen to CD8 Lymphocytes and Alter CD8 T Cell Differentiation into IL-10-Producing Phenotypes. Journal of Immunology 2009; 183(-5-):3033-3039. >>> Interleukin-3; Mice (transgenic); 7 days; Animal info (C57BL/6, OT-I TCR-transgenic, IL-4KO B6); immunology.

Q0798 Miksa,M., Wu,R.Q., Dong,W.F., Komura,H., Amin,D., Ji,Y.X., Wang,Z.M., Wang,H.C., Ravikumar,T.S., Tracey,K.J., Wang,P. Immature Dendritic Cell-Derived Exosomes Rescue Septic Animals Via Milk Fat Globule Epidermal Growth Factor VIII. Journal of Immunology 2009; 183(-9-):5983-5990. >>> Epidermal growth factor VIII, milk fat globule, recomb. murine; IV (jugular); Rat; 2001D; 20 hours; Controls received mp w/ PBS; immunology; animal info (male, Sprague-Dawley).

Q0530 Morris,G.P., Brown,N.K., Kong,Y.C.M. Naturally-existing CD4+CD25+Foxp3+ regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen. Journal of Autoimmunity 2009; 33(-1-):68-76. >>> Thyroid stimulating hormone, bovine; IP; Mice; 2001; 7 days; Controls received sham operation with saline mp; animal info (female, CBA/J (H2k), 6-10 wks old); immunology.

Q0571 Roa,J., Garcia-Galiano,D., Varela,L., Sanchez-Garrido,M.A., Pineda,R., Castellano,J.M., Ruiz-Pino,F., Romero,M., Aguilar,E., Lopez,M., Gaytan,F., Dieguez,C., Pinilla,L., Tena-Sempere,M. The Mammalian Target of Rapamycin as Novel Central Regulator of Puberty Onset via Modulation of Hypothalamic Kiss1 System. Endocrinology 2009; 150(-11-):5016-5026. >>> Leucine, L-; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (Wistar, female); immunology.

P9241 Amantini,C., Mosca,M., Lucciarini,R., Perfumi,M.C., Santoni,G. Thiorphan-induced survival and proliferation of rat thymocytes by activation of Akt/survivin pathway and inhibition of caspase-3 activity. Journal of Pharmacology and Experimental Therapeutics 2008; 327(-1-):215-225. >>> Thiorphan; SR140333; substance P; Ethanol; water, distilled; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle or no treatment; no stress (see pg. 216); enzyme inhibitor (peptidase (NEP/CD10)); immunology; animal info (male, Wistar, 21 days old); 10% EtOH used; "We chose to delivery SP, SR140333, and Thiorphan by a continuous route of administration, because this modality closely mimics the pathophysiological state observed in immune-mediated diseases." (p. 216).

P8843 Changelian,P.S., Moshinsky,D., Kuhn,C.F., Flanagan,M.E., Munchhof,M.J., Harris,T.M., Doty,J.L., Sun,J.M., Kent,C.R., Magnuson,K.S., Perregaux,D.G., Sawyer,P.S., Kudlacz,E.M. The specificity of JAK3 kinase inhibitors. Blood 2008; 111(-4-):2155-2157. >>> PF-956980; PEG 300; SC; Mice; 2004; 14 days; Dose-response (Fig. 1); enzyme inhibitor (JAK3); immunology.

P8859 Koo,J.W., Duman,R.S. IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-2-):751-756. >>> Interleukin-1 receptor antagonist; BSA; PBS; CSF/CNS; Rat; 2004; 21 days; Controls received mp w/ vehicle; immunology; ALZET brain infusion kit 2 used; animal info (adult, male, Sprague Dawley, 230-250g.).

P9430 McCandless,E.E., Zhangt,B., Diamondt,M.S., Klein,R.S. CXCR4 antagonism increases T cell trafficking in the central nervous system and improves survival from West Nile virus encephalitis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-32-):11270-11275. >>> AMD 3100; PBS; SC; Mice; 2002; 13-16 days; Controls received mp w/ vehicle; half-life (p. 11272) 0.9 hours in rodent; immunology; animal info (male, C57BL/6, 5 wks old); CXCR4 antagonist; "To ensure sufficient levels of the antagonist throughout the 16-day experiment period, AMD3100 was delivered via a s.c. osmotic infusion pump." (p. 11271).

P9256 Mishra,N.C., Rir-Sima-Ah,J., Langley,R.J., Singh,S.P., Pena-Philippides,J.C., Koga,T., Razani-Boroujerdi,S., Hutt,J., Campen,M., Kim,K.C., Tesfaigzi,Y., Sopori,M.L. Nicotine primarily suppresses lung Th2 but not goblet cell and muscle cell responses to allergens. Journal of Immunology 2008; 180(-11-):7655-7663. >>> Nicotine; SC; Rat; 10-20 days; Controls received mp w/ saline; immunology; animal info (female, Brown Norway, 6-8 wks old).

P9280 Mitamura,T., Yamada,A., Hanaoka,K., Asano,M., Niwa,T., Seki,J. Effect of pharmacokinetic profile on the pancreatic toxicity and efficacy of tacrolimus in rats. JOURNAL OF TOXICOLOGICAL SCIENCES 2008; 33(-5-):575-584. >>> FK-506; Propylene glycol; ethanol; castor oil, hydrogenated; IV (femoral); Rat; 2002; 9, 14 days; Controls received mp w/ vehicle; functionality of mp verified by blood drug concentration; dose-response (fig. 4); comparison of bolus IV injections vs. mp; immunology; toxicology; animal info (male, Jcl:SD, 9 wks old: Fischer, WKAtt, 8 wks old; skin allograft); 15% EtOH used; "The sustained blood concentration of tacrolimus (via ALZET) with an AUC similar to that of bolus administration was better tolerated and had the same efficacy...These results indicate that the sustained-release formulation of tacrolimus has the potential to improve the safety of tacrolimus." (p. 583); also known as FK-506.

P9436 Reuter,S., Velic,A., Edemir,B., Schroeter,R., Pavenstaedt,H., Gabriels,G., Bleich,M., Schlatter,E. Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2008; 456(-6-):1075-1084. >>> NHE-3I4167; Water, sterile; SC; Rat; 2001; 5 days; Replacement therapy (nephrectomy); immunology; animal info (male, Lewis, Lewis Brown Norway, 240-290 g., kidney transplantation); nephrology; Nat/Ht Exchanger type 3 Inihibitor #4167).

P9235 Sonderegger,I., Iezzi,G., Maier,R., Schmitz,N., Kurrer,M., Kopf,M. GM-CSF mediates autoimmunity by enhancing IL-6-dependent Th17 cell development and survival. Journal of Experimental Medicine 2008; 205(-10-):2281-2294. >>> Interleukin-6, recomb. human; SC; Mice (transgenic); mice; 2001; 7 days; Controls received sham surgery; immunology; peptides; animal info (BALB/c, wt, GM-CSF -/-, DO11.10 TG, 8-12 wks old); "To ensure a continuous supply, we implanted osmotic minipump containing IL-6 in mice." (p. 2289).

P9236 Uchiyama,Y., Yoshida,H., Koike,N., Hayakawa,N., Sugita,A., Nishimura,T., Mihara,M. Anti-IL-6 receptor antibody increases blood IL-6 level via the blockade of IL-6 clearance, but not via the induction of IL-6 production. INTERNATIONAL IMMUNOPHARMACOLOGY 2008; 8(-11-):1595-1601. >>> Interleukin-6, recomb. human; SC; Mice; 1002; 9-10 days; Functionality of mp verified by serum hIL-6 levels; immunology; peptides; animal info (male, C57BL/6, IL-6 deficient).

P9263 Wang,L., van Panhuys,N., Hu-Li,J., Kim,S.H., Le Gros,G., Min,B.K. Blimp-1 induced by IL-4 plays a critical role in suppressing IL-2 production in activated CD4 T cells. Journal of Immunology 2008; 181(-8-):5249-5256. >>> Interleukin-4; ovalbumin; SC; Mice; 7 days; Immunology, animal info (CD45.1, C57BL/6).

P8509 Bhasin,M., Wu,M.Z., Tsirka,S.E. Modulation of microglial/macrophage activation by macrophage inhibitory factor (TKP) or tuftsin (TKPR) attenuates the disease course of experimental autoimmune encephalomyelitis. BMC IMMUNOLOGY 2007; 8(--):U1-U16. >>> Tuftsin; macrophage inhibitory factor; SC; Mice; 2002; 30 days; Controls received mp w/ PBS; pumps replaced after 14 days; enzyme inhibitor (ACE); immunology; peptides; animal info (female, tPA -/- or wt, 6-10 weeks old); neurodegenerative (Multiple Sclerosis).

P8446 Coenen,J.J.A., Koenen,H.J.P.M., van Rijssen,E., Kasran,A., Boon,L., Hilbrands,L.B., Joosten,I. Rapamycin, not cyclosporine, permits thymic generation and peripheral preservation of CD4+ CD25+ FoxP3+ T cells. Bone Marrow Transplantation 2007; 39(-9-):537-545. >>> Cyclosporin A; SC; Mice; 2004; 7,14,28 days; Controls received mp w/PBS; dose-response (fig. 1); cardiovascular; immunology; animal info (C3H (H-2K), BALB/C (H-2d), 8-12 wks old).

P8610 Dietrich,T., Onderka,J., Bock,F., Kruse,F.E., Vossmeyer,D., Stragies,R., Zahn,G., Cursiefen,C. Inhibition of Inflammatory Lymphangiogenesis by Integrin alpha 5 Blockade. American Journal of Pathology 2007; 171(-1-):361-372. >>> JSM6427; JSM6424; SC; Mice; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by mp and plasma levels analysis; dose-response (p. 368); pumps replaced after one week; half-life (p. 363) "rapid;" cardiovascular; immunology; animal info (BALB/C, 6 weeks old, suture-induced inflammatory corneal neovascularization); integrin a5B1 and avB3 antagonists; angiogenesis; lymphangiogenesis.

P8629 Fitzgerald,D.C., Ciric,B., Touil,T., Harle,H., Grammatikopolou,J., Das Sarma,J., Gran,B., Zhang,G.X., Rostami,A. Suppressive effect of IL-27 on encephalitogenic Th17 cellsAnd the effector phase of experimental autoimmune encephalomyelitis1. Journal of Immunology 2007; 179(-5-):3268-3275. >>> Interleukin-27, recomb. mouse; PBS; SC; Mice; 7 days; Controls received mp w/ vehicle; animal info (female, C57BL/6, 8-12 wks old); neurodegenerative (multiple sclerosis); "initially we tested i.p. injection of recombinant carrier-free IL-27 however, these experiments did not provide reproducible findings. To achieve continual, consistent delivery of cytokine throughout the treatment period, we implanted osmotic minipumps." (p. 3273); immunology.

P8441 Kabashima,K., Shiraishi,N., Sugita,K., Mori,T., Onoue,A., Kobayashi,M., Sakabe,J.I., Yoshiki,R., Tamamura,H., Fujii,N., Inaba,K., Tokura,Y. CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. American Journal of Pathology 2007; 171(-4-):1249-1257. >>> TN14003, 4F-benzoyl; Saline; SC; Mice; 1007D; 2,4,5,7 days; Controls received mp w/ vehicle; immunology; animal info (female, C57BL/6, 8 wks old).

P8416 Khalaf,W.F., Yang,F.C., Chen,S., White,H., Bessler,W., Ingram,D.A., Clapp,D.W. K-ras is critical for modulating multiple c-kit-mediated cellular functions in wild-type and Nf1+/- mast cells. Journal of Immunology 2007; 178(-4-):2527-2534. >>> Kit-ligand; SC; Mice; 7 days; Controls received mp w/ PBS; replacement therapy (irradiation, fetal liver cell transfer); immunology; animal info (W/Wv).

P8966 Marcu,A.C., Paccione,K.E., Barbee,R.W., Diegelmann,R.F., Ivatury,R.R., Ward,K.R., Loria,R.M. Androstenetriol immunomodulation improves survival in a severe trauma Hemorrhage shock model. JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE 2007; 63(-3-):662-669. >>> Morphine; SC; Rat; 1003D; 6-48 hours; Immunology; post op. care (antibiotic ointment); animal info (male, Sprague Dawley, 300-375 g.); mp used to provide "clinically relevant amounts of analgesia during the entire experiment." (p. 663).

P8965 Matsuno,H., Yoshida,K., Ochiai,A., Okamoto,M. Requirement of methotrexate in combination with anti-tumor necrosis factor alpha therapy for adequate suppression of osteoclastogenesis in rheumatoid arthritis. Journal of Rheumatology 2007; 34(-12-):2326-2333. >>> Influximab; immunoglobulin G, human; SC; Mice (SCID); 2 weeks; Controls received mp w/ human IgG; immunology; animal info (SCID-HuRAg-pit, 6-7 wks old; TNF-alpha inhibitor.

P8557 Oh,K., Shen,T., Le Gros,G., Min,B. Induction of Th2 type immunity in a mouse system reveals a novel immunoregulatory role of basophils. Blood 2007; 109(-7-):2921-2927. >>> Interleukin-3; SC; Mice; mice (transgenic); 7 days; Controls received mp w/ PBS or no treatment; immunology; peptides; animal info (C57BL/6, BIO.A Rag -/-); "Sought an alternative strategy to generate basophils in vivo by administering IL-3 into mice via a miniosmotic pump." (p. 2923).

P8463 Shen,X.Z., Li,P., Weiss,D., Fuchs,S., Xiao,H.D., Adams,J.A., Williams,I.R., Capecchi,M.R., Taylor,W.R., Bernstein,K.E. Mice with enhanced macrophage angiotensin-converting enzyme are resistant to melanoma. American Journal of Pathology 2007; 170(-6-):2122-2134. >>> Captopril; losartan; SC; Mice; 2 weeks; Functionality of mp verified by systolic blood pressure; enzyme inhibitor (ACE); cancer (melanoma); immunology; animal info (C56BL/6, wt, ACE 10/10, 8wks old).

P8310 Singh,A.K., Gudehithlu,K.P., Litbarg,N.O., Sethupathi,P., Arruda,J.A.L., Dunea,G. Transplanting fragments of diabetic pancreas into activated omentum gives rise to new insulin producing cells. Biochemical and Biophysical Research Communications 2007; 355(-1-):258-262. >>> Insulin; SC; Rat; 28 days; Replacement therapy (STZ-induced diabetes, pancreatectomy and autotransplanted into omentum; immunology; animal info (male, Sprague-Dawley, 150-200g.); nephrology.

R0248 Tsutsumi,H., Tamamura,H., Fujii,N. Inhibitors of the chemokine receptor CXCR4: Chemotherapy of AIDS, metastatic cancer, leukemia and rheumatoid arthritis. Letters in Drug Design&Discovery 2007; 4(-1-):20-26. >>> TN14003, 4F-benzoyl-; SC; Mice; Mice (SCID); ; Cancer (breast carcinoma); immunology; peptides; CXCR4 antagonist; review, see p. 21, 23.

P8367 Wranning,C.A., El-Akouri,R.R., Groth,K., Moelne,J., Parra,A.K., Braennstroem,M. Rejection of the transplanted uterus is suppressed by cyclosporine A in a semi-allogeneic mouse model. Human Reproduction 2007; 22(-2-):372-379. >>> Cyclosporin A; Propylene glycol; SC; Mice; 1007D; 7-10 days; Controls received mp w/ vehicle, or no treatment; functionality of mp verified by serum CyA levels; dose-response (Table 1); immunology; post op.care (buprenorphin, carprofen, cefuroxine); animal info (female, C57BL/6, B6CBAF1, 6-8 wks old, uterine transplant).

P8747 Zou,W., Guo,Q., Wang,E., Cai,J., Cheng,Z. Intrathecal morphine suppresses immune function in rats with inflammatory-induced pain. Journal of International Medical Research 2007; 35(-5-):626-636. >>> Morphine; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001; 1 week; Controls received mp w/ vehicle; dose-response (fig. 1); immunology; post op. care (natrium penicillin); animal info (male, Sprague-Dawley, 250-300g).

P8394 Earle,S.A., El-Haddad,A., Patel,M.B., Ruiz,P., Pham,S.M., Majetschak,M. Prolongation of skin graft survival by exogenous ubiquitin. Transplantation 2006; 82(-11-):1544-1546. >>> Ubiquitin; albumin; Saline, normal; IP; Mice; 2002; 5,14 days; Controls received mp w/ vehicle, or albumin or no treatment; functionality of mp verified by serum ubiquitin levles; dose-response (fig. 1); no stress (see pg. 1546); immunology; peptides; animal info ( C3H/HEJ, DBA2, 22g., skin grafts); "Ubiquitin administration was well tolerated and body weight was similar in both groups" (pg. 1546).

P7610 Feng,P., Rahim,R.T., Cowan,A., Liu-Chen,L.Y., Peng,X.H., Gaughan,J., Meissler,JJ Jr, Adler,M.W., Eisenstein,T.K. Effects of mu, kappa or delta opioids administered by pellet or pump on oral Salmonella infection and gastrointestinal transit. European Journal of Pharmacology 2006; 534(-1-3-):250-257. >>> Morphine sulfate; enkephalin analog DPDPE; U50,488H; deltorphin II, D-ala2-; Saline, pyrogen free; SC; Mice; 1003D; 48 hours; Controls received mp w/ vehicle; dose-response (fig 1); comparison of pellets vs. mp; immunology; animal info (female, 6 wk old); mp primed 4 hours in 37 C saline; "morphine pellet potently exacerbated oral salmonella infection, but morphine given by pump, at doses which were immunosuppresive had a substantially lesser effect (of infection)." (p. 251). "Further, we and others have found that morphine pellets induce sepsis in mice." (p. 251).

P7782 Glezer,I., Lapointe,A., Rivest,S. Innate immunity triggers oligodendrocyte progenitor reactivity and confines damages to brain injuries. FASEB Journal 2006; 20(-2-):U405-U430. >>> Endotoxin, LPS; MK-801; Saline, sterile; CSF/CNS; Rat; 2004; 3, 7 days; Controls received mp w/ vehicle; immunology; animal info (male, Sprague-Dawley, 250g.).

P8143 Griffiths,G.D., Hornby,R.J., Jagger,C.P., Brown,A.P., Stoten,A., Pearce,P.C., Scott,L., Pritchard,D.I. Development of methods to measure humoral immune responses against selected antigens in the common marmoset (Callithrix jacchus) and the effect of pyridostigmine bromide administration. INTERNATIONAL IMMUNOPHARMACOLOGY 2006; 6(-12-):1755-1764. >>> Pyridostigmine bromide; Saline, sterile isotonic; SC; Marmoset; 28 days; Controls received mp w/ vehicle; no stress (see p.1759,1762); immunology; animal info (male, female, 300-500g.); mp primed 40 hours; "delivery by pump ensured the animals would receive an appropriate dose of the drug over the desired time period.", oral delivery "would introduce unacceptable stress into the experiment and presentation in food was discounted because of difficulties in estimating the dose administered." (p.1757).

P8084 Hirayama,S., Shiraishi,T., Shirakusa,T., Higuchi,T., Miller,E.J. Prevention of neutrophil migration ameliorates rat lung allograft rejection. MOLECULAR MEDICINE 2006; 12(-9-10-):208-213. >>> Antileukinate; PBS; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; comparison of bolus IM injection vs. mp; immunology; peptides; ischemia (lung); animal info (male, Fischer 344, 250-300g, left lung transplantation); mp primed 4 hrs in 37 celcius saline.

P8082 Hornby,R.J., Pearce,P.C., Bowditch,A.P., Scott,L., Griffiths,G.D. Multiple vaccine and pyridostigmine bromide interactions in the common marmoset Callithrix jacchus: Immunological and endocrinological effects. INTERNATIONAL IMMUNOPHARMACOLOGY 2006; 6(-12-):1765-1779. >>> Pyridostigmine bromide; Saline, sterile isotonic; SC; Marmoset; 2004; 28 days; Controls received mp w/ vehicle; no stress (see p.1776); immunology; animal info (female, vasectomized male, 331-565g. 2-5.5 yrs. old); mp primed 40 hours.

P7881 Hu,J.S., Freeman,C.M., Stolberg,V.R., Chiu,B.C., Bridger,G.J., Fricker,S.P., Lukacs,N.W., Chensue,S.W. AMD3465, a novel CXCR4 receptor antagonist, abrogates schistosomal antigen-elicited (type-2) pulmonary granuloma formation. American Journal of Pathology 2006; 169(-2-):424-432. >>> AMD 3465; PBS; IP; Mice; 5 days; Controls received mp w/ vehicle; dose-response (fig. 1); no stress (see pg. 426); immunology; animal info (female, CBA/J, 6-8 wk old).

P7468 Kim,J.H., Bronson,C.L., Hayton,W.L., Radmacher,M.D., Roopenian,D.C., Robinson,J.M., Anderson,C.L. Albumin turnover: FcRn-mediated recycling saves as much albumin from degradation as the liver produces. American Journal of Physiology-Gastrointestinal and Liver Physiology 2006; 290(-2-):G352-G360. >>> Leucine, 3H-; SC; Mice; 1002; 2 weeks; Controls received mp w/ agent and were WT mice; functionality of mp verified by residual volume and steady state plasma concentrations of labeled proteins; comparison of IV bolus injections vs. mp; half-life (p. G353)<15 minutes; immunology; animal info (C57BL/6J, FcRn a-chain, knockout, male 28 g); mp primed 4 hours in 37C isotonic saline.

P7856 Liu,Y.R., Liu,J., Tetzlaff,W., Paty,D.W., Cynader,M.S. Biliverdin reductase, a major physiologic cytoprotectant, suppresses experimental autoimmune encephalomyelitis. Free Radical Biology and Medicine 2006; 40(-6-):960-967. >>> CSF, artificial; CSF, artificial; gentamicin; CSF/CNS (intrathecal, subarachnoid space); Rat; 2004; ; Immunology; animal info (male, Lewis, 200g., EAE (experimental autoimmune encephalomyelitis)); freshly prepared antioxidant enzymes were delivered intrathecally once daily through the PE tubing via Hamilton syringe on days 13-17, mp then reconnected to keep tube clear; multiple sclerosis model; pump used to maintain catheter patency.

P8205 McCandless,E.E., Wang,Q., Woerner,B.M., Harper,J.M., Klein,R.S. CXCL12 limits inflammation by localizing mononuclear infiltrates to the perivascular space during experimental autoimmune encephalomyelitis. Journal of Immunology 2006; 177(-11-):8053-8064. >>> AMD 3100; PBS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response (fig.3); immunology; animal info (C57BL/6, 8-12 wk old, female).

P8261 Si,Y., Ciccone,S., Yang,F.C., Yuan,J., Zeng,D., Chen,S., van de Vrugt,H.J., Critser,J., Arwert,F., Haneline,L.S., Clapp,D.W. Continuous in vivo infusion of interferon-gamma (IFN-gamma) enhances engraftment of syngeneic wild-type cells in Fanca-/- and Fancg-/- mice. Blood 2006; 108(-13-):4283-4287. >>> Interferon-gamma, mouse; PBS; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; no stress (see pg. 4286); immunology; peptides; animal info (C57BL/6).

P9203 Siddiqui,A.M., Cui,X.X., Wu,R.Q., Dong,W.F  Crit.Care Med. 2006; 34(-7-):1874-1882. >>> Curcumin; GW9662; DMSO; Dulbecco's modified eagle medium; IV (jugular); Rat; 2ML1; 3 days; Controls received mp w/vehicle; immunology; animal info (male, Sprague-Dawley; 275-325 g); phytochemical derived from the root Curcuma Conga (ginger family); 50% DMSO/ 50% DMEM; GW9662 via same pump; no apparent toxicity to DMSO observed; Curcumin administered IV due to low circulating plasma levels following gavage or gastric pump.

R0249 Tamamura,H., Tsutsumi,H., Fujii,N. The chemokine receptor CXCR4 as a therapeutic target for several diseases. MINI-REVIEWS IN MEDICINAL CHEMISTRY 2006; 6(-9-):989-995. >>> TN14003, 4F-benzoyl-; SC; Mice; mice (SCID); ; Cancer (breast carcinoma); immunology; peptides; Review, see p. 990, ref. #22; p. 992, red. # 56.

P7791 Ulusal,B.G., Ulusal,A.E., Hung,L.M., Wei,F.C. The effect of A2A adenosine receptor agonist on composite tissue allotransplant survival: An in vivo preliminary study. Journal of Surgical Research 2006; 131(-2-):261-266. >>> CGS 21680; SC; Rat; 7 days; Controls received no treatment; no stress (see pg. 263); immunology; animal info (Brown Norway, Lewis, Hindlimb transplantation); A-2a adenosine receptor agonist; "The animals tolerated the minipumps well." (pg.263).

P7375 Bao,L.H., Osawe,I., Puri,T., Lambris,J.D., Haas,M., Quigg,R.J. C5a promotes development of experimental lupus nephritis which can be blocked with a specific receptor antagonist. European Journal of Immunology 2005; 35(-8-):2496-2506. >>> Peptide, C5a receptor antagonist; DMSO; saline; SC; Mice (transgenic); 2001; 6 weeks; Controls received mp w/ vehicle; pumps replaced every week; immunology; peptides; 50% DMSO; C5a anaphylatoxin; "subcutaneous mini-osmotic pumps were used to deliver C5aRa or vehicle continuously, thereby achieving steady-state concentrations and avoiding the need for frequent injections." (p. 2498).

P7493 Cao,H., Jung,M., Stamatoyannopoulos,G. Hydroxamide derivatives of short-chain fatty acid have erythropoietic activity and induce gamma gene expression in vivo. Experimental Hematology 2005; 33(-12-):1443-1449. >>> Hydroxamate, butyryl; hydroxamate, propionyl; hydroxamic acid, suberoylanilide; hydroxamic acid, subericbis; SC; Mice (transgenic); 2001; 7 days; Controls received mp w/ PBS; dose-response; enzyme inhibitor (histone deacetylase); cardiovascular; immunology.

P6950 Chen,W.H., Langer,R.M., Janczewska,S., Furian,L., Geary,R., Qu,X.M., Wang,M., Verani,R., Condon,T., Stecker,K., Bennett,C.F., Stepkowski,S.M. Methoxyethyl-modified intercellular adhesion molecule-1 antisense phosphorothiateoligonucleotides inhibit allograft rejection, ischemic-reperfusion injury, and cyclosporine-induced nephrotoxicity. Transplantation 2005; 79(-4-):401-408. >>> Oligonucleotide, antisense; Saline; IV; Rat; 7 days; Comparison of gavage and IV injections vs. mp; antisense (phosphorothioate/phosphodiester); transplantation; 14 d pump used; immunology.

P7124 Day,Y.J., Li,Y.S., Rieger,J.M., Ramos,S.I., Okusa,M.D., Linden,J. A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury1. Journal of Immunology 2005; 174(-8-):5040-5046. >>> ATL146e; SC; Mice; 1003D; 24 hours; Controls received mp w/ vehicle; immunology; ischemia (hepatic); Chimeric mice of rA24 receptor created by irradiaton and bone marrow transplant.

P7498 De Klerck,B., Geboes,L., Hatse,S., Kelchtermans,H., Meyvis,Y., Vermeire,K., Bridger,G., Billiau,A., Schols,D., Matthys,P. Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis. ARTHRITIS RESEARCH&THERAPY 2005; 7(-6-):R1208-R1220. >>> AMD 3100; SC; Mice; 2002; 14 days; Controls received mp w/ PBS; immunology; animal info (male, 8-12 weeks).

P7316 Dokur,M., Chen,C.P., Advis,J.P., Sarkar,D.K. Beta-endorphin modulation of interferon-gamma, perforin and granzyme B levels in splenic NK cells: Effects of ethanol. Journal of Neuroimmunology 2005; 166(-1-2-):29-38. >>> Endorphin, B-; CSF/CNS (paraventricular nucleus); Rat; 2002; 18 hours; Controls received mp w/ aCSF; immunology; peptides.

P7432 Ellis,S., Mouihate,A., Pittman,Q.J. Early life immune challenge alters innate immune responses to lipopolysaccharide: implications for host defense as adults. FASEB Journal 2005; 19(-8-):U826-U842. >>> Corticosterone; PEG; IP; Rat; 2001; 1 week; Replacement therapy (adrenalectomy); no stress (see pg. 4); immunology; post op. care (derapen); "the survival rate of this procedure (ADX + mp) was 100% with animals having normal body temperature within 24 hours after the surgery.".

P7715 Fukada,K., Koyanagi,M., Arimura,Y., Ogiuchi,H., Uchiyama,T., Yagi,J. CD28 is required for induction and maintenance of immunological memory in toxin-reactive CD4+ T cells in vivo. Cellular Immunology 2005; 238(-2-):103-112. >>> Antigen, staphylococcal enterotoxin A; SC; Mice; 2001; 7 days; Immunology; post op. care (penicillin G); animal info (C57BL/6, female, B-12 wk. old, CD28 -/- or B6 wt).

P7589 Gemma,C., Fister,M., Hudson,C., Bickford,P.C. Improvement of memory for context by inhibition of caspase-1 in aged rats. European Journal of Neuroscience 2005; 22(-7-):1751-1756. >>> YVAD-CMK, Ac-; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ 0.6% DMSO in saline; functionality of mp verified by mp weight (not suggested by manufacturer); enzyme inhibitor (caspase -1); immunology; animal info (young, aged, make, Fischer 344 g); mp primed 48 hr in 37 C sterile saline.

P6865 Goodridge,H.S., Marshall,F.A., Else,K.J., Houston,K.M., Egan,C., Al-Riyami,L., Liew,F.Y., Harnett,W., Harnett,M.M. Immunomodulation via novel use of TLR4 by the filarial nematode phosphorylcholine-containing secreted product, ES-62. Journal of Immunology 2005; 174(-1-):284-293. >>> ES-62; PBS; SC; Mice; 2002; 14 days; Controls received mp w/ vehicle; dose-response (fig. 1); immunology.

P7217 Griffiths,G.D., Telford,G., Hooi,D.S.W., Cook,D.L., Wilkinson,L.J., Green,C.A., Pritchard,D.I. A T-cell-dependent humoral immune response is preserved during the administration of the nerve agent pre-treatment pyridostigmine bromide in a murine model. INTERNATIONAL IMMUNOPHARMACOLOGY 2005; 5(-3-):525-540. >>> Pyridostigmine bromide; Saline, sterile isotonic; SC; Mice; 2004; 28 days; Controls received mp w/ vehicle; enzyme inhibitor (cholinesterase); immunology; mp primed in saline at 37 degrees celsius for 48 hours; "PB administration was achieved by 28-day mini-osmotic pumps thus removing the requirement for multiple oral dosing and the associated stress this may have caused." (p. 536).

P7665 Guzman,E.A., Chen,Y.H., Langowski,J.L., De Guzman,A., Lo,H.L., Walter,B., Muller,H.K., Walker,A.M., Owen,L.B. Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin. Journal of Neuroimmunology 2005; 170(-1-2-):31-40. >>> Prolactin, recomb.; prolactin, S179D; Saline; SC; Mice; 2004; 21 days; Controls received mp w/ vehicle; immunology; animal info (male, C3H/HeJ or C3H/HeJ-TnFsF6gld, 10-12 wk. old).

P7163 Inokuchi,Y., Morohashi,T., Kawana,I., Nagashima,Y., Kihara,M., Umemura,S. Amelioration of 2,4,6-trinitrobenzene sulphonic acid induced colitis in angiotensinogen gene knockout mice. Gut 2005; 54(-3-):349-356. >>> Losartan; SC; Mice (knockout); 21 days; Controls received mp w/ normal saline, comparison of oral delivery vs. mp, immunology.

P7663 Kaplin,A.I., Deshpande,D.M., Scott,E., Krishnan,C., Carmen,J.S., Shats,I., Martinez,T., Drummond,J., Dike,S., Pletnikov,M., Keswani,S.C., Moran,T.H., Pardo,C.A., Calabresi,P.A., Kerr,D.A. IL-6 induces regionally selective spinal cord injury in patients with the neuroinflammatory disorder transverse myelitis. Journal of Clinical Investigation 2005; 115(-10-):2731-2741. >>> Interleukin 6, recomb. rat; PBS; BSA; CSF/CNS (intrathecal, subarachnoid space); Rat; 1007D; 7 days; Controls received mp w/ vehicle; immunology; neurodegenerative (transverse myelitis, spinal cord injury); animal info (Sprague-Dawley, adult); SCI.

P7329 Kim,S.J., Winter,K., Nian,C., Tsuneoka,M., Koda,Y., McIntosh,C.H.S. Glucose-dependent insulinotropic polypeptide (GIP) stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxo1, and down-regulation of bax expression. Journal of Biological Chemistry 2005; 280(-23-):22297-22307. >>> Glucose-dependent insulinotropic polypeptide (1-42), synthetic porcine; IP; Rat; 2 weeks; Replacement therapy (diabetic); immunology; peptides.

P7249 Lay,A.J., Liang,Z., Rosen,E.D., Castellino,F.J. Mice with a severe deficiency in protein C display prothrombotic and proinflammatory phenotypes and compromised maternal reproductive capabilities. Journal of Clinical Investigation 2005; 115(-6-):1552-1561. >>> Hirudin; IP; Mice (transgenic); 3 days; Controls received mp w/ saline; no stress (see pg. 1555); enzyme inhibitor (thrombin); cardiovascular; immunology.

P7184 Mathian,A., Weinberg,A., Gallegos,M., Banchereau,J., Koutouzov,S. IFN-alpha induces early lethal lupus in preautoimmune (New Zealand black x New Zealand White)F1 but not in BALB/c mice1. Journal of Immunology 2005; 174(-5-):2499-2506. >>> Interferon, mouse; PBS; BSA; SC; Mice; 1002; 8-10 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 weeks; immunology.

P7263 Min,B., Yamane,H., Hu-Li,J., Paul,W.E. Spontaneous and homeostatic proliferation of CD4 T cells are regulated by different mechanisms. Journal of Immunology 2005; 174(-10-):6039-6044. >>> Interleukin-7, recomb. mouse; Mice; mice (transgenic); 7, 28 days; Controls received mp w/ PBS; immunology; peptides.

P7537 Morishima,Y., Ishii,Y., Kimura,T., Shibuya,A., Shibuya,K., Hegab,A.E., Iizuka,T., Kiwamoto,T., Matsuno,Y., Sakamoto,T., Nomura,A., Taniguchi,M., Sekizawa,K. Suppression of eosinophilic airway inflammation by treatment with alpha-galactosylceramide. European Journal of Immunology 2005; 35(-10-):2803-2814. >>> Galactosylceramide, a-; Saline; SC; Mice; 29 days; Controls received mp w/ vehicle; comparison of SC injection vs. mp; immunology; animal info (BALB/c, 7-12 wk. old).

P7186 Newman,R., Cuan,N., Hampartzoumian,T., Connor,S.J., Lloyd,A.R., Grimm,M.C. Vasoactive intestinal peptide impairs leucocyte migration but fails to modify experimental murine colitis. Clinical and Experimental Immunology 2005; 139(-3-):411-420. >>> Vasoactive intestinal peptide; SC; Mice; 3, 7 days; Controls received mp w/ saline; functionality of mp verified by VIP plasma levels; comparison of IP injections vs. mp; immunology; peptides; TNBS-induced colitis.

P7203 Rochman,I., Paul,W.E., Ben-Sasson,S.Z. IL-6 increases primed cell expansion and survival. Journal of Immunology 2005; 174(-8-):4761-4767. >>> Cytochrome c, pigeon; intereukin-6, recomb.mouse; PBS; Mice; 7 days; Dose-response (Fig. 1); immunology; peptides.

P7460 Roychowdhury,S., Blaser,B.W., Freud,A.G., Katz,K., Bhatt,D., Ferketich,A.K., Bergdall,V., Kusewitt,D., Baiocchi,R.A., Caligiuri,M.A. IL-15 but not IL-2 rapidly induces lethal xenogeneic graft-versus-host disease. Blood 2005; 106(-7-):2433-2435. >>> Interleukin-15, recomb. human; interleukin-2, recomb. human; PBS; albumin, human; SC; Mice (SCID); 1007D; 10 days; Controls received mp w/ vehicle; immunology; animal info (female, CB17, hu-PBL-SCID, 8-12 weeks old).

P7302 Singer,H.S., Mink,J.W., Loiselle,C.R., Burke,K.A., Ruchkina,I., Morshed,S., Parveen,S., Leckman,J.F., Hallett,J.J., Lombroso,P.J. Microinfusion of antineuronal antibodies into rodent striatum: Failure to differentiate between elevated and low titers. Journal of Neuroimmunology 2005; 163(-1-2-):8-14. >>> Serum, human; dye trypan blue; PBS; CSF/CNS (ventrolateral striatum); Rat; 1007D; 2002; 96 hours; Controls received mp w/ vehicle, or sham surgery; functionality of mp verified by observing progression of Typan blue in the catheter; immunology; cannula placement confirmed by India ink dye injection; 65 ul serum placed in catheter, Trypan blue solution placed in mp; serum continued high or low levels of antineuronal antibodies.

P7507 Wieland,C.W., Florquin,S., Chan,E.D., Leemans,J.C., Weijer,S., Verbon,A., Fantuzzi,G., van der Poll,T. Pulmonary Mycobacterium tuberculosis infection in leptin-deficient ob/ob mice. International Immunology 2005; 17(-11-):1399-1408. >>> Leptin; SC; Mice; 2004; 18 days; Controls received mp w/ saline; immunology; peptides; animal info (female, C57BL/6, ob/ob or WT, 6-8 wk old).

P7574 Yamashita,N., Tashimo,H., Ishida,H., Matsuo,Y., Arai,H., Nagase,H., Adachi,T., Ohta,K. Role of insulin-like growth factor-I in allergen-induced airway inflammation and remodeling. Cellular Immunology 2005; 235(-2-):85-91. >>> Antibody, anti-IGF-I, murine; immunoglobulin G, goat; Saline, physiological; SC; Mice; 2 weeks; Controls received mp w/ goat IgG; dose-response (fig 2); immunology; animal info (male, 10-12 wk old).