References on the Administration of Insulin Using ALZET^® Osmotic Pumps

Q0655 Polakof,S., Medale,F., Larroquet,L., Vachot,C., Corraze,G., Panserat,S. Insulin Stimulates Lipogenesis and Attenuates Beta-Oxidation in White Adipose Tissue of Fed Rainbow Trout. Lipids 2011; 46(-2-):189-199. >>> Insulin, bovine; IP; Fish (Rainbow trout); 5, 11 days; Controls received mp w/ saline; animal info (rainbow trout, 200 g); post op. care (antibiotic gel).

Q0506 Adam,A., Leclair,P., Montpas,N., Koumbadinga,G.A., Bachelard,H., Marceau,F. Altered cardiac bradykinin metabolism in experimental diabetes caused by the variations of angiotensin-converting enzyme and other peptidases. Neuropeptides 2010; 44(-2-):69-75. >>> Insulin, regular; IP; Rat; 2ML2; 7 days; Controls received no treatment; animal info (diabetic, Sprague Dawley, 125-150 g.); diabetes.

Q0322 Annaba,F., Ma,K., Kumar,P., Dudeja,A.K., Kineman,R.D., Shneider,B.L., Saksena,S., Gill,R.K., Alrefai,W.A. Ileal apical Na(+)-dependent bile acid transporter ASBT is upregulated in rats with diabetes mellitus induced by low doses of streptozotocin. American Journal of Physiology-Gastrointestinal and Liver Physiology 2010; 299(-4-):G898-G906. >>> Insulin, human; SC; Rat; 2ML1; 3 days; Animal info (Sprague-Dawley, males, 7-8 wks old, 200-250 g); diabetes; "Upon insulin treatment from subcutaneous osmotic pumps, glucose levels were restored to normal" pg G904; streptozotocin-induced diabetes.

P9976 Nordquist,L., Shimada,K., Ishii,T., Furuya,D.T., Kamikawa,A., Kimura,K. Proinsulin C-peptide prevents type-1 diabetes-induced decrease of renal Na+-K+-ATPase alpha-1-subunit in rats. DIABETES-METABOLISM RESEARCH AND REVIEWS 2010; 26(-3-):193-199. >>> C-peptide, human; insulin, recomb. human; SC; Rat; 2ML2; 1 weeks; Controls received mp w/ saline; animal info (male, Sprague Dawley, 15 wks old, 350-450 g.); replacement therapy (STZ-induced Diabetes).

Q0182 Polakof,S., Skiba-Cassy,S., Choubert,G., Panserat,S. Insulin-induced hypoglycaemia is co-ordinately regulated by liver and muscle during acute and chronic insulin stimulation in rainbow trout (Oncorhynchus mykiss). Journal of Experimental Biology 2010; 213(-9-):1443-1452. >>> Insulin, bovine; Saline; IP; Fish (trout); 1003D; 4 days; Controls received mp w/ vehicle; peptides; animal info (rainbow trout, 170 g); post op. care (antibiotic ointment); comparison of IP injections vs. mp; 17 degrees C; endocrinology.

Q0030 Searls,Y.M., Loganathan,R., Smirnova,I.V., Stehno-Bittel,L. Intracellular Ca2+ regulating proteins in vascular smooth muscle cells are altered with type 1 diabetes due to the direct effects of hyperglycemia. Cardiovascular Diabetology 2010; 9(-;-):U1-U17. >>> Insulin; SC; Rat; 2004; 28 days; Controls received mp w/saline; peptides; animal info (male, DR-BB Wistar, 21-26 days old); comparison of SC injections vs. mp; replacement therapy (anti-RT6 mAB-induced diabetes); endocrinology; Humulin R, Humulin U and Humulin N used;.

Q0939 Park,S., Hong,S.M., Ahn,I.S. Long-Term Intracerebroventricular Infusion of Insulin, but Not Glucose, Modulates Body Weight and Hepatic Insulin Sensitivity by Modifying the Hypothalamic Insulin Signaling Pathway in Type 2 Diabetic Rats. Neuroendocrinology 2009; 89(-4-):387-399. >>> Insulin; glucose; CSF/CNS; Rat; 4 weeks; Controls received mp w/ artificial CSF; animal info (Sprague-Dawley, male, 215 g); one group contained insulin + glucose; pump implanted after 1 week recovery period.

P9941 Polakof,S., Skiba-Cassy,S., Panserat,S. Glucose homeostasis is impaired by a paradoxical interaction between metformin and insulin in carnivorous rainbow trout. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-6-):R1769-R1776. >>> Metformin; insulin, bovine; SC; Fish; 1007D; ; Controls received mp w/ saline; post op.care (antibiotic gel); animal info (rainbow trout); one group contained metformin and insulin.

Q0568 Xu,J., Kirigiti,M.A., Grove,K.L., Smith,M.S. Regulation of Food Intake and Gonadotropin-Releasing Hormone/Luteinizing Hormone during Lactation: Role of Insulin and Leptin. Endocrinology 2009; 150(-9-):4231-4240. >>> Insulin, human; leptin, rat; Saline; SC; Rat; 1003D; 48 hours; Controls received mp w/ vehicle; animal info (d 8-9 post partum, 6 d post ovariectomy); multiple pumps per animal (2); replacement therapy (ovariectomized).

P8767 Johansson,M.E., Andersson,I.J., Alexanderson,C., Skott,O., Holmaeng,A., Bergstroem,G. Hyperinsulinemic rats are normotensive but sensitized to angiotensin II. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 294(-4-):R1240-R1247. >>> Insulin; losartan; SC; Rat; 6 weeks; Controls received mp w/ no treatment; cardiovascular; peptides; animal info (female, Sprague Dawley, 180g.); endocrinology.

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P9376 Rodriguez,W.E., Sen,U., Tyagi,N., Kumar,M., Carneal,G., Aggrawal,D., Newsome,J., Tyagi,S.C. PPAR gamma agonist normalizes glomerular filtration rate, tissue levels of homocysteine, and attenuates endothelial-myocyte uncoupling in alloxan induced diabetic mice. International Journal of Biological Sciences 2008; 4(-4-):236-244. >>> FITC-Insulin; IP; Mice; 2001D; 24 hours; Replacement therapy (Alloxan-induced diabetes); cardiovascular; animal info (male, C57BL/6J); endocrinology.

P9611 Takimoto,C., Kumagai,H., Osaka,M., Sakata,K., Onami,T., Kamayachi,T., Iigaya,K., Hayashi,K., Saruta,T., Itoh,H. Candesartan and Insulin Reduce Renal Sympathetic Nerve Activity in Hypertensive Type 1 Diabetic Rats. HYPERTENSION RESEARCH 2008; 31(-10-):1941-1951. >>> Insulin; SC; Rat; 2 weeks; Controls received mp w/ vehicle; animal info (male, 13 wks old, SHR); replacement therapy (STZ-induced Diabetes).

P8758 Warne,J.P., Foster,M.T., Horneman,H.F., Pecoraro,N.C., de Jong,H.K., Ginsberg,A.B., Akana,S.F., Dallman,M.F. The gastroduodenal branch of the common hepatic vagus regulates voluntary lard intake, fat deposition, and plasma metabolites in streptozotocin-diabetic rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-1-):E190-E200. >>> Insulin, Humulin R U500; IV (superior mesenteric); Rat; 2002; 10 days; Controls received mp w/ saline; replacement therapy (STZ-induced Diabetes, gastroduodenal branch vagotomy).

P8167 Bidani,A.K., Picken,M., Hacioglu,R., Williamson,G., Griffin,K.A. Spontaneously reduced blood pressure load in the rat streptozotocin-induced diabetes model: potential pathogenetic relevance. American Journal of Physiology-Renal Physiology 2007; 292(-2-):F647-F654. >>> Insulin; SC; Rat; 16-20, 36-40 weeks; Replacement therapy (STZ-induced Diabetes); long-term study; pumps replaced every 4 weeks; peptides; animal info (male, Sprague-Dawley, 8 weeks old, 250 grams).

P8683 Garcia-San Frutos,M., Fernandez-Agullo,T., De Solis,A.J., Andres,A., Arribas,C., Carrascosa,J.M., Ros,M. Impaired central insulin response in aged Wistar rats: Role of adiposity. Endocrinology 2007; 148(-11-):5238-5247. >>> Insulin, human; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; dose-response (fig. 1, p. 5241); peptides; animal info (Wistar, 3, 8, 24 month, male); endocrinology.

P8516 Hong,L.Z., Hsieh,P.S. Hyperinsulinemia Instead of Insulin Resistance Induces Baroreflex Dysfunction in Chronic Insulin-Infused Rats. American Journal of Hypertension 2007; 20(-4-):451-458. >>> Insulin, porcine; Glycerin; ethanol; SC; Rat; 2002; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 14 days; post op. care (penicillin); animal info (male, Sprague-Dawley, 200-250 grams); 50% ETOH used [manufacturer suggests 15% or less ETOH; for pump compatibility purposes].

P8073 Hoppe,C.C., Evans,R.G., Moritz,K.M., Cullen-McEwen,L.A., Fitzgerald,S.M., Dowling,J., Bertram,J.F. Combined prenatal and postnatal protein restriction influences adult kidney structure, function, and arterial pressure. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 292(-1-):R462-R469. >>> Insulin; aminohippuric acid, p-; Radio-isotopes; 3H tracer; SC; Rat; 2ML1; 3 days; Cardiovascular; animal info (Sprague-Dawley, male, 132 days old, 340-425g.); nephrology.

P8734 Kim,S.J., Nian,C., McIntosh,C.H.S. Resistin is a key mediator of glucose-dependent insulinotropic polypeptide (GIP) stimulation of lipoprotein lipase (LPL) activity in adipocytes. Journal of Biological Chemistry 2007; 282(-47-):34139-34147. >>> Glucose-dependent insulinotropic polypeptide; PBS; IP; Rat; 2 weeks; Controls received mp w/ vehicle; peptide; animals info (VDF Zucker, Fa/Fa or lean; 8 weeks old).

P8149 Kovacs,P., Morales,J.C., Karkanias,G.B. The effect of diabetes and centrally administered insulin on anterior hypothalamic estrogen receptor alpha immunoreactivity. ACTA DIABETOLOGICA 2007; 44(-1-):38-44. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (STZ-induced Diabetes, ovariectomy); peptides; animal info (female, Sprague-Dawley, 175-200g.); tissue perfusion (ventricle).

P8972 Prabhakar,S., Starnes,J., Shi,S., Lonis,B., Tran,R. Diabetic nephropathy is associated with oxidative stress and decreased renal nitric oxide production. Journal of the American Society of Nephrology 2007; 18(-11-):2945-2952. >>> Insulin, porcine; Glycerine, ethanol; SC; Rat; 2004; 12 weeks; Controls received no treatment; long-term study; pumps replaced every 4 wks; multiple pumps per animal (2); animal info (ZSF1, 8 wks old, 200-250 g.); 50% ethanol used.

P8310 Singh,A.K., Gudehithlu,K.P., Litbarg,N.O., Sethupathi,P., Arruda,J.A.L., Dunea,G. Transplanting fragments of diabetic pancreas into activated omentum gives rise to new insulin producing cells. Biochemical and Biophysical Research Communications 2007; 355(-1-):258-262. >>> Insulin; SC; Rat; 28 days; Replacement therapy (STZ-induced diabetes, pancreatectomy and autotransplanted into omentum; immunology; animal info (male, Sprague-Dawley, 150-200g.); nephrology.

P8957 Tiwari,S., Halagappa,V.K.M., Riazi,S., Hu,X.Q., Ecelbarger,C.A. Reduced expression of insulin receptors in the kidneys of insulin-resistant rats. Journal of the American Society of Nephrology 2007; 18(-10-):2661-2671. >>> Insulin, recomb. human; SC; Rat; 2002; 28 days; Controls received sham operation; pumps replaced; peptides; animal info (Sprague Dawley, 250-280 g.); nephrology; Research Diets.

P8701 Warne,J.P., Horneman,H.F., Ginsberg,A.B., Pecoraro,N.C., Foster,M.T., Akana,S.F., Dallman,M.F. Mapping brain c-fos immunoreactivity after insulin-induced voluntary lard intake: Insulin- and lard-associated patterns. Journal of Neuroendocrinology 2007; 19(-10-):794-808. >>> Insulin, Humulin R U500; Saline; IV (jugular); Rat; 2002; 10 days; Controls received mp w/ vehicle; replacement therapy (STZ-induced diabetes); no stress (see pg. 797); peptides; post op. care (Ketoprofen); animal info (male, Sprague-Dawley, 300g); "The presence of an osmotic mini-pump at either site did not cause any obvious signs of pains or discomfort in the rats. " (p. 797).

P8620 Warne,J.P., Foster,M.T., Horneman,H.E., Pecoraro,N.C., Ginsberg,A.B., Akana,S.F., Dallman,M.F. Afferent signalling through the common hepatic branch of the vagus inhibits voluntary lard intake and modifies plasma metabolite levels in rats. JOURNAL OF PHYSIOLOGY-LONDON 2007; 583(-2-):455-467. >>> Insulin, Humulin R U500; IV (superior mesenteric); Rat; 2002; 10 days; Controls received mp w/ saline; replacement therapy (STZ-induced diabetes); peptides; animal info (male, Sprague-Dawley, 300 g); catheter position verified.

P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).

P8304 Chakrabarty,S., Miller,B.T., Collins,T.J., Nagamani,M. Ovarian dysfunction in peripubertal hyperinsulinemia. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION 2006; 13(-2-):122-129. >>> Insulin; SC; Rat; 2004; 4 weeks; Controls received mp w/ physiological saline; no stress (see pg. 126); peptides; animal info (female, Sprague-Dawley, 28 days old); "This method of drug delivery eliminates the stress and trauma of daily injections." (p. 126).

P7451 Fekete,C., Singru,P.S., Sanchez,E., Sarkar,S., Christoffolete,M.A., Riberio,R.S., Rand,W.M., Emerson,C.H., Bianco,A.C., Lechan,R.M. Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. Endocrinology 2006; 147(-1-):520-529. >>> Leptin, recomb. mouse; insulin; glucose; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by agent levels in CSF+ serum; peptides; post op. care (bacitracin ointment); animal info (male Sprague-Dawley, 200-250 g); cannula placement confirmed by light microscope.

P7758 Song,J., Hu,X.Q., Riazi,S., Tiwari,S., Wade,J.B., Ecelbarger,C.A. Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats. American Journal of Physiology-Renal Physiology 2006; 290(-5-):F1055-F1064. >>> Insulin, human; Water; SC; Rat; 2002; 28 days; Controls received sham surgery; pumps replaced at 2 weeks; no stress (see pg. F1056); animal info (male, Sprague-Dawley, 250-280g.); Humulin-R; continuous radiotelemetry to record blood pressure.

P8004 Toba,H., Gomyo,E., Miki,S., Shimizu,T., Yoshimura,A., Inoue,R., Sawai,N., Tsukamoto,R., Asayama,J., Kobara,M., Nakata,T. Hyperinsulinaemia increases the gene expression of endothelial nitric oxide synthase and the phosphatidylinositol 3-kinase/Akt pathway in rat aorta. Clinical and Experimental Pharmacology and Physiology 2006; 33(-5-6-):440-447. >>> Insulin; Saline; SC; Rat; 2 weeks; Controls received mp w/ vehicle; insulin plasma levels taken; cardiovascular; peptides; animal info (male, Wistar, 280-300 grams).

P7928 Toth,C., Brussee,V., Martinez,J.A., McDonald,D., Zochodne,D.W. Rescue and regeneration of injured peripheral nerve axons by intrathecal insulin. Neuroscience 2006; 139(-2-):429-449. >>> Insulin; Saline; CSF/CNS (intrathecal, lumbar, peroneal nerve); Rat; 2002; 2 weeks; Controls received mp w/ vehicle; peptides; animal info (adult male, Sprague-Dawley, 250-300 grams); silicone catheters (0.012" x 0.025") used between L6-S1 vertebrae; nerve perfusion chamber used for nerve infusion; nerve injury; India Ink in pumps to verify delivery; behavioral testing.

P7985 Warne,J.P., Horneman,H.F., Wick,E.C., Bhargava,A., Pecoraro,N.C., Ginsberg,A.B., Akana,S.F., Dallman,M.F. Comparison of superior mesenteric versus jugular venous infusions of insulin in streptozotocin-diabetic rats on the choice of caloric intake, body weight, and fat stores. Endocrinology 2006; 147(-11-):5443-5451. >>> Insulin, methylene blue; Saline; IV (jugular); Rat; 2002; ; Functionality of mp verified by dye infusion; replacement therapy (diabetic); no stress (see pg. 5444); good methods; peptides; animal info (male, Sprague-Dawley, 275 grams); diabetes; PE-60 fused to PE-5.

P7673 Dragonas,C., Mueller,A., Maltaris,T., Kraemer,P., Dimmler,A., Jaeger,W., Beckmann,M.W., Dittrich,R. Role of insulin in the progression of ovarian sex cord stromal tumors in rats. Journal of Cancer Research and Clinical Oncology 2005; 131(-11-):751-757. >>> Insulin; SC; Rat; 28, 56, 84 days; Controls received mp w/ saline; replacement therapy (ovariectomy, STZ-induced diabetes); long-term study; pumps replaced every 28 days; no stress (see pg.753); cancer (sex cord stomal); animal info (female, Lewis, 60 days old, 150-210 g).

P6956 El Midaoui,A., De Champlain,J. Effects of glucose and insulin on the development of oxidative stress and hypertension in animal models of type 1 and type 2 diabetes. Journal of Hypertension 2005; 23(-3-):581-588. >>> Insulin; Saline; glutamic acid; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; insulin plasma levels taken; cardiovascular; peptides; diabetes; 0.7% glutamic acid added to prevent insulin aggregation; replacement therapy (STZ-induced Diabetes).

P7358 El Midaoui,A., Ongali,B., Petcu,M., Rodi,D., De Champlain,J., Neugebauer,W., Couture,R. Increases of spinal kinin receptor binding sites in two rat models of insulin resistance. Peptides 2005; 26(-8-):1323-1330. >>> Insulin; SC; Rat; 2002; 2 weeks; Peptides; diabetes.

P6949 Fowler,M., Virostko,J., Chen,Z.Y., Poffenberger,G., Radhika,A., Brissova,M., Shiota,M., Nicholson,W.E., Shi,Y.B., Hirshberg,B., Harlan,D.M., Jansen,E.D., Powers,A.C. Assessment of pancreatic islet mass after islet transplantation using in vivo bioluminescence imaging. Transplantation 2005; 79(-7-):768-776. >>> Insulin, human; SC; Mice (SCID); 1002; 7,10 days; Diabetes; bioluminescence imaging (BLI); IVIS 200 system used after pumps were removed.

P7329 Kim,S.J., Winter,K., Nian,C., Tsuneoka,M., Koda,Y., McIntosh,C.H.S. Glucose-dependent insulinotropic polypeptide (GIP) stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxo1, and down-regulation of bax expression. Journal of Biological Chemistry 2005; 280(-23-):22297-22307. >>> Glucose-dependent insulinotropic polypeptide (1-42), synthetic porcine; IP; Rat; 2 weeks; Replacement therapy (diabetic); immunology; peptides.

P6903 Nyberg,J., Anderson,M.F., Meister,B., Alborn,A.M., Stroem,A.K., Brederlau,A., Illerskog,A.C., Nilsson,O., Kieffer,T.J., Hietala,M.A., Ricksten,A., Eriksson,P.S. Glucose-dependent insulinotropic polypeptide is expressed in adult hippocampus and induces progenitor cell proliferation. Journal of Neuroscience 2005; 25(-7-):1816-1825. >>> Glucose-dependent insulinotropic polypeptide; PBS; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2; peptides; GIP.

R0211 Banks,W.A. The source of cerebral insulin. European Journal of Pharmacology 2004; 490(-1-3-):5-12. >>> Insulin, human; SC; Mice; ; Dose-response (Fig. 1); peptides; pg. 7 fig.1.

P7046 Bilodeau,M., Tousignant,J., Ethier,C., Rocheleau,B., Raymond,V.A., Lapointe,R. Anti-apoptotic effect of insulin on normal hepatocytes in vitro and in vivo. Apoptosis 2004; 9(-5-):609-617. >>> Insulin; HEPES buffer; IV (portal); Rat; 2001; 2,7 days; Controls received mp w/ vehicle; functionality of mp verified by degree of phosphorylation of insulin receptor; replacement therapy (liver atrophy); dose-response.

P7007 Crowley,W.R., Ramoz,G., Torto,R., Kalra,S.P. Role of leptin in orexigenic neuropeptide expression during lactation in rats. Journal of Neuroendocrinology 2004; 16(-7-):637-644. >>> Leptin, recomb. rat; insulin, bovine; Saline; SC; Rat; 2001; 3 days; Controls received mp w/ vehicle; peptides.

P6641 Figlewicz,D.P., Zavosh,A., Sexton,T., Neumaier,J.F. Catabolic action of insulin in rat arcuate nucleus is not enhanced by exogenous "tub" expression. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2004; 286(-6-):E1004-E1010. >>> Insulin; CSF, artificial; CSF/CNS (arcuate nucleus); Rat; 1007D; 6 days; Controls received mp w/ vehicle; dose-response (fig. 4); peptides; multiple pumps per animal (2).

P6678 Figlewicz,D.P., Bennett,J., Evans,S.B., Kaiyala,K., Sipols,A.J., Benoit,S.C. Intraventricular insulin and leptin reverse place preference conditioned with high-fat diet in rats. Behavioral Neuroscience 2004; 118(-3-):479-487. >>> Leptin; insulin; CSF; CSF/CNS (third ventricle); Rat; 7,14 days; Controls received mp w/ vehicle; peptides; behavioral study; one week and two week pump used; models not listed.

P6568 Fredersdorf,S., Thumann,C., Ulucan,C., Griese,D.P., Luchner,A., Riegger,G.A.J., Kromer,E.P., Weil,J. Myocardial hypertrophy and enhanced left ventricular contractility in Zucker diabetic fatty rats. CARDIOVASCULAR PATHOLOGY 2004; 13(-1-):11-19. >>> Insulin; SC; Rat; 2ML2; 2ML4; 6 weeks; Functionality of mp verified by serum glucose levels; pumps replaced after 2 weeks; cardiovascular; insulin used was actrapid Hm u500.

P6673 Lambert,A.J., Merry,B.J. Effect of caloric restriction on mitochondrial reactive oxygen species production and bioenergetics: reversal by insulin. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2004; 286(-1-):R71-R79. >>> Insulin; Saline; acetic acid; SC; Rat; 2002; 2 weeks; Insulin plasma levels; peptides; post op. care (buprenorphin injection); aging.

P6031 Kobayashi,T., Kaneda,A., Kamata,K. Possible involvement of IGF-1 receptor and IGF-binding protein in insulin-induced enhancement of noradrenaline response in diabetic rat aorta. British Journal of Pharmacology 2003; 140(-2-):285-294. >>> Insulin; Saline; SC; Rat; 2ML2; 2 weeks; Insulin plasma levels taken; cardiovascular; peptides; diabetes; replacement therapy (STZ-induced Diabetes).

P6236 Kovacs,P., Morales,J.C., Karkanias,G.B. Central insulin administration maintains reproductive behavior in diabetic female rats. Neuroendocrinology 2003; 78(-2-):90-95. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); no stress (see pg. 91).

P6073 Miyanaga,F., Ogawa,Y., Ebihara,K., Hidaka,S., Tanaka,T., Hayashi,S., Masuzaki,H., Nakao,K. Leptin as an adjunct of insulin therapy in insulin-deficient diabetes. Diabetologia 2003; 46(-10-):1329-1337. >>> Leptin; insulin, human; Saline; SC; Mice (transgenic); 1007D; ; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; paper incorrectly states pump model as a 1002D; replacement therapy (STZ-induced Diabetes).

P6015 Song,J., Knepper,M.A., Verbalis,J.G., Ecelbarger,C.A. Increased renal ENaC subunit and sodium transporter abundances in streptozotocin-induced type 1 diabetes. American Journal of Physiology-Renal Physiology 2003; 285(-6-):F1125-F1137. >>> Insulin; SC; Rat; 2002; 14 days; Insulin plasma levels taken; peptides; timeline of pump usage, p. F1127 (Fig. A); replacement therapy (STZ-induced Diabetes).

P5114 Granda,T.G., Velasco,A. Effects of insulin administration in diabetic rats on food and drink rhythmic schedules. BIOLOGICAL RHYTHM RESEARCH 2002; 33(-1-):1-14. >>> Insulin; SC; rat; 2ML4; 4 weeks; controls received mp w/ vehicle; replacement therapy (STZ-induced Diabetes).

P5498 Granda,T.G., Velasco,A. Sodium and potassium clearance rhythmicity in diabetic rats with insulin treatment. LIFE SCIENCES 2002; 71(-21-):2475-2487. >>> Insulin; SC; Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; replacement therapy (streptozotocin-induced diabetes); peptides.

P7430 Hewson,A.K., Tung,L.Y., Connell,D.W., Tookman,L., Dickson,S.L. The rat arcuate nucleus integrates peripheral signals provided by leptin, insulin, and a ghrelin mimetic. Diabetes 2002/12; 51(-12-):3412-3419. >>> Insulin, recomb. human; leptin, recomb. murine; Saline; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; replacement therapy (fa/fa obese); ALZET brain infusion kit 2 used; peptides; mp primed overnight in 37 degree C saline.

P6230 Kovacs,P., Parlow,A.F., Karkanias,G.B. Effect of centrally administered insulin on gonadotropin-releasing hormone neuron activity and luteinizing hormone surge in the diabetic female rat. Neuroendocrinology 2002; 76(-6-):357-365. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; 2002; 8-12 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); endocrinology.

P5825 Obici,S., Zhang,B.B., Karkanias,G., Rossetti,L. Hypothalamic insulin signaling is required for inhibition of glucose production. Nature Medicine 2002; 8(-12-):1376-1382. >>> Insulin, porcine; CSF, artifical; CSF/CNS (third ventricle); Rat; 2002; 3-7 days; Controls received mp w/ vehicle; stability verified by past experiment >11 days stable p.322; peptides; two cannulae used, one in the 3rd ventricle&the other in the right ventricle; 3 day recovery; two cannula systems used so that acute administration of compounds (via lateral ventricle) would not interfere w/ continuous infusion of a second solution (via the third ventricle).

P5134 Thompson,C.I., Munford,J.W., Buell,E.H., Karry,R.J., Lee,C.T., Morgan,B.L., Radnovich,A.J. Plasma constituents and mortality in rat pups given chronic insulin via injection, pellet, or osmotic minipump. Can.J Physiol Pharmacol 2002/3; 80(--):180-192. >>> Insulin, bovine; Saline; SC; rat (neonate); 1002; 10 days; Animal info (neonates, 9 days old), controls received mp w/ vehicle; functionality of mp verified by plasma insulin levels and residual aspiration; comparison of pellet vs. sc injections vs. mp; no stress (see p. 186); "The minipumps appeared to be well tolerated and caused no obvious difficulty with nursing" (p. 186); teratology; peptides; "...pups tolerate insulin more readily from a minipump than from a pellet..." (p. 190) "pellets caused high mortality within 24 h" (abstract); "Hypothermia is safe, and it has been shown to be an effective technique for inducing anesthesia in neonatal and preweaning rats. We therefore used moderate hypothermia to subcutaneously implant the osmotic minipumps..." p. 186; "It took approximately 1.5 minutes to implant the minipump in each pup..." p. 186; "The present studies indicate that subcutaneous insulin pellets are not suitable for this purpose (chronic insulin delivery in rat pups), since rat pups do not tolerate them well." p. 191.

P4803 Fang,T.-C., Wu,C.C., Huang,W.C. Inhibition of nitric oxide synthesis accentuates blood pressure elevation in hyperinsulinemic rats.. Journal of Hypertension 2001; 19(-7-):1255-1262. >>> Insulin, porcine;; Glycerin; Ethanol;; SC;; rat;; 2002;; 6 weeks;; Controls received mp w/ vehicle; functionality of mp verified by removing, measuring residual volume; long-term study, pumps replaced after 14 days and 28 days; vehicle was 50:50 glycerin, ethanol mix;.

P5766 Hidaka,S., Yoshimatsu,H., Kondou,S., Oka,K., Tsuruta,Y., Sakino,H., Itateyama,I., Noguchi,H., Himeno,K., Okamoto,K., Teshima,Y., Okeda,T., Sakata,T. Hypoleptinemia, but not hypoinsulinemia, induces hyperphagia in streptozotocin-induced diabetic rats. J Neurochem 2001; 77(-4-):993-1000. >>> Insulin; Leptin; CSF/CNS (third ventricle); Rat; 2001; 6 days; Controls received mp w/ saline; peptides; leptin was recomb. murine; guide cannula w/ stylet used; replacement therapy (STZ-induced Diabetes).

P4980 Soriano,F.G., Pacher,P., Mabley,J., Liaudet,L., Szabo,C. Rapid reversal of the diabetic endothelial dysfunction by pharmacological inhibition of poly(ADP-ribose) polymerase. Circ.Res 2001/10/12; 89(-8-):684-691. >>> Insulin, bovine; SC; Mice; 2004; ; 1 or 4 weeks; replacement therapy (streptozotocin-induced diabetes); incorrectly states pump model was 1004.

P5236 Tabata,H. Peripheral neuropathy in B6C3F1 mice and SD rats induced by chronic intermittent insulin hypoglycemia. Drug Chem.Toxicol. 2000/8; 23(-3-):485-496. >>> Insulin, bovine; Water; HCl; SC; Rat; Mice; 1007D, 2002; 2 weeks; Controls received mp w/ vehicle; functionality veryfied by blood glucose levels; dose-response (p.490); pumps replaced at intervals of 5 to 7 days; peptides; 1007D pumps used in mice; 2002 pumps used in rats; toxicology.