References on the Administration of Insulin Using ALZET® Osmotic Pumps

16219 Keunhee Oh,, Sae Rom Kim,, Dae-Kyum Kim,, Myung Won Seo,, Changjin Lee,, Hak Mo Lee,, Ju-Eun Oh,, Eun Young Choi,, Dong-Sup Lee,, Yong Song Gho,, Kyong Soo Park  ACS Nano 2015; 9(-12-):11718-11727. >>> Insulin; SC; Mice (NOD); animal info (BALB/c, NOD male mice, aged 7-10 wks); functionality of mp verified by blood glucose levels; diabetes; Dose (0.14 units/day);.

16172 Schaschkow, A., Mura, C., Dal, S., Langlois, A., Seyfritz, E., Sookhareea, C., Bietiger, W., Peronet, C., Jeandidier, N., Pinget, M., Sigrist, S., Maillard, E. Impact of the Type of Continuous Insulin Administration on Metabolism in a Diabetic Rat Model. J Diabetes Res 2016; 2016(--):8310516. >>> insulin; buffer solution; SC; rat; animal info (male, Lewis, STZ injection); comparison of injection of insulin vs mp; post op. care (Baytril 10 mg/kg QD for 7 days); diabetes; pumps primed for 24 hours in 37C saline;“…continuous insulin delivery by pumps restored normoglycaemia, which induced the reduction of both reactive oxygen species and macrophage infiltration into the liver and omentum. Injections controlled the glucose levels for only a short period of time and therefore tissue stress and inflammation were elevated.” pg 1; “pumps require no daily injection and facilitate rat follow- up. Well-being of the animals and the homogeneity of the results permit researchers to limit the numbers of animals and experiments used to build solid and reproducible results.” pg 8; Dose (4 IU/day);.

16018 Grant, C. W., Moran-Paul, C. M., Duclos, S. K., Guberski, D. L., Arreaza-Rubin, G., Spain, L. M. Testing agents for prevention or reversal of type 1 diabetes in rodents. PLoS One 2013; 8(-8-):e72989. >>> insulin; SC; rat; animal info: LEW.1WR1, male and female, 21-25 days old; functionality of mp verified by glycosuria, blood glucose levels>250 mg/dl; dose response: Fig. 6; paper mentions a downside of continuous infusion of insulin: a drawback of this approach was that animals could become hypoglycemic or on the low end of the normal range at higher frequencies than was observed when injections were used; insulin (0.25 U/day);.

15880 Templeman, N. M., Clee, S. M., Johnson, J. D. Suppression of hyperinsulinaemia in growing female mice provides long-term protection against obesity. Diabetologia 2015; 58(-10-):2392-402. >>> Insulin2, murine peptide; SC; Mice; 2004; Controls received mp w/ vehicle; animal info (Ins1 -/-; 25 weeks old); functionality of mp verified by plasma insulin; peptides; diabetes; Dose (0.1 U/day);.

15754 Stephanie Dal,, Nathalie Jeandidier,, Elodie Seyfritz,, William Bietiger,, Claude Pe´ronet,, Franc¸ois Moreau,, Michel Pinget,, Elisa Maillard, and Se´verine Sigrist  EXPERIMENTAL BIOLOGY AND MEDICINE 2016; 241(--):184-192. >>> Insulin; SC; Rats; 2006; animal info (male, Wistar, 180-200g, STZ); immunology; diabetes;"The intensive subcutaneous insulin administrationperformed using a mini pump in our study led to animprovement in the metabolic control of diabetic rats, as confirmed by a decrease in fructosamine levels and an increase in body weight after four weeks of treatment. Moreover, blood insulin concentration was maintained at the same level throughout the study attesting to the efficiency of this therapy."pg 189; Dose (2 UI/200g/day);.

15716 Christian W. Grant,, Catherine M. Moran-Paul,, Shane K. Duclos,, Dennis L. Guberski,, Guillermo Arreaza-Rubín,, Lisa M. Spain  PLoS One 2013; 8(-8-):1-14. >>> ISO-092, insulin; SC; rat; not specified; LEW.1WR1, male and female, 21-25 days old, rats; functionality of mp verified by glycosuria, blood glucose levels>250 mg/dl; Fig. 6 for dose-response; dose: insulin (0.25 U/day);.

14340 Pandey, G., Makhija, E., George, N., Chakravarti, B., Godbole, M.M., Ecelbarger, C.M., Tiwari, S., Tiwari, S. Insulin Regulates Nitric Oxide Production in the Kidney Collecting Duct Cells. Journal of Biological Chemistry 2015; 590(--):5582-5591. >>> Insulin; SC; Mice; 1007D; Animal info (male, C57BL6J, 5 months old); cardiovascular;.

14301 Mehrotra, P., Jamwal, S.V., Saquib, N.M., Sinha, N., Siddiqui, Z., Manivel, V., Chatterjee, S., Rao, K.V.S., Rao, K.V.S. Pathogenicity of Mycobacterium tuberculosis Is Expressed by Regulating Metabolic Thresholds of the Host Macrophage. PLoS Pathogens 2014; 10(--):U589-U608. >>> Insulin, bovine; UK5099; SC; Mice; Controls received mp w/ vehicle; animal info (female, BALB/C, 4-6 weeks old); immunology; diabetes;.

14650 Adzovic, L., Lynn, A.E., D'Angelo, H.M., Crockett, A.M., Kaercher, R.M., Royer, S.E., Hopp, S.C., Wenk, G.L., Wenk, G.L. Insulin improves memory and reduces chronic neuroinflammation in the hippocampus of young but not aged brains. Journal of Neuroinflammation 2015; 12(--):U1-U10. >>> Insulin, recombinant cat; endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; Controls received mp w/ vehicle; animal info (male, F-344, 3 months old, 21 months old); bilateral cannula used; behavioral testing (morris water maze); tissue perfusion (fourth ventricle); bilateral infusion; used tygon tubing to attach cannula to pump;.

14635 Dodd, G.T., Decherf, S., Loh, K., Simonds, S.E., Wiede, F., Balland, E., Merry, T.L., Muenzberg, H., Zhang, Z.Y., Kahn, B.B., Neel, B.G., Bence, K.K., Andrews, Z.B., Cowley, M.A., Tiganis, T., Tiganis, T. Leptin and Insulin Act on POMC Neurons to Promote the Browning of White Fat. Cell 2015; 160(--):88-104. >>> Leptin; insulin, human; CSF/CNS; CSF/CNS (intra-arcuate nucleus of the hypothalamus); Mice; 1002; Control animals received mp w/ vehicle; animal info (8 wks old, C57BL/6); ALZET brain infusion kit (3) used; Plastics One bilateral cannula used with PEG tubing and a Y connector.

13629 Tsuchiya, K., Accili, D. Liver Sinusoidal Endothelial Cells Link Hyperinsulinemia to Hepatic Insulin Resistance. Diabetes 2013; 62(-5-):1478-1489. >>> Insulin; PBS; Mice; 1003D; Controls received mp w/ vehicle; animal info (male, VECKO); functionality of mp verified by plasma insulin; no stress (see pg.1480); peptides; diabetes;.

13475 Palei, A.C., Spradley, F.T., Granger, J.P. Euglycemic Hyperinsulinemia Increases Blood Pressure in Pregnant Rats Independent of Placental Antiangiogenic and Inflammatory Factors. American Journal of Hypertension 2013; 26(-11-):1445-1451. >>> Insulin; SC; Rat (pregnant); 1007D; Animal info (female, Sprague Dawley); functionality of mp verified by serum insulin levels; cardiovascular; peptides; diabetes;.

13247 Frikke-Schmidt, H., Pedersen, T.A., Fledelius, C., Olsen, G.S., Hellerstein, M. Adipose Weight Gain during Chronic Insulin Treatment of Mice Results from Changes in Lipid Storage without Affecting De Novo Synthesis of Palmitate. PLoS One 2013; 8(-9-):U1010-U1016. >>> Insulin, human; SC; Mice; 1002; Controls received mp w/ phosphate buffer; animal info (15 weeks old, female, C57BL6/J).

13243 Hokke, S.N., Armitage, J.A., Puelles, V.G., Short, K.M., Jones, L., Smyth, I.M., Bertram, J.F., Cullen-McEwen, L.A. Altered Ureteric Branching Morphogenesis and Nephron Endowment in Offspring of Diabetic and Insulin-Treated Pregnancy. PLoS One 2013; 8(-3-):U453-U461. >>> Insulin; Saline; Mice; 1007D; Controls received mp w/ saline; Peptides; animal info (8-12wk, C57BL/6J, mice (pregnant)); mp used to infuse insulin to study the effect of hyperglycemia and glycemic control on ureteric branching during pregnancy; diabetes;.

13221 Thomas, C.M., Yong, Q.C., Seqqat, R., Chandel, N., Feldman, D.L., Baker, K.M., Kumar, R. Direct renin inhibition prevents cardiac dysfunction in a diabetic mouse model: comparison with an angiotensin receptor antagonist and angiotensin-converting enzyme inhibitor. Clinical Science 2013; 124(-7-8-):529-541. >>> Insulin; aliskiren (renin inhibitor); benazeprilat (ACEi); valsartan (ARB); streptozotocin; Saline; SC; Mice; 1004; Controls received mp w/vehicle, or (0.1M sodium citrate buffer (pH 4.5)); cardiovascular; animal info (male, C57b16/J, 12 weeks, blood glucose>250 mg/dl); pumps replaced every 4 weeks; enzyme inhibitor (renin); long-term study; diabetes;.

13179 Novikova, L., Smirnova, I.V., Rawal, S., Dotson, A.L., Benedict, S.H., Stehno-Bittel, L. Variations in Rodent Models of Type 1 Diabetes: Islet Morphology. JOURNAL OF DIABETES RESEARCH 2013; ;(-;-):U1-U13. >>> Insulin; SC; Rat; Peptides; animal info (BBDR, rat, 23-25 days); functionality of mp verified by measuring blood glucose levels; diabetes;.

13020 Grant, C.W., Duclos, S.K., Moran-Paul, C.M., Yahalom, B., Tirabassi, R.S., rreaza-Rubin, G., Spain, L.M., Guberski, D.L. Development of Standardized Insulin Treatment Protocols for Spontaneous Rodent Models of Type 1 Diabetes. COMPARATIVE MEDICINE 2012; 62(-5-):381-390. >>> Insulin; SC; Mice (NOD); 1002; Animal info (diabetic, NOD); Humulin R used; wound clips used; diabetes; dose response (Table 1);"Compared with injected insulin, osmotic insulin pumps provided superior glycemic control, although diabetic NOD mice did experience events of hypoglycemia. diabetes;.

13016 Fredersdorf, S., Thumann, C., Zimmermann, W.H., Vetter, R., Graf, T., Luchner, A., Riegger, G.A.J., Schunkert, H., Eschenhagen, T., Weil, J. Increased myocardial SERCA expression in early type 2 diabetes mellitus is insulin dependent: In vivo and in vitro data. Cardiovascular Diabetology 2012; 11(-;-):U1-U11. >>> Insulin; SC; Rat; 2ML2; 2ML4; animal info (male, Zucker, 118 g, 13 wks old); pumps replaced after 2 weeks; diabetes;"Pumps were changed after 2 weeks and the insulin dose was adapted to normalize blood glucose levels"pg 3; long-term study;.

12806 Li, L.J., Garikepati, R.M., Tsukerman, S., Kohan, D., Wade, J.B., Tiwari, S., Ecelbarger, C.M. Reduced ENaC activity and blood pressure in mice with genetic knockout of the insulin receptor in the renal collecting duct. American Journal of Physiology-Renal Physiology 2013; 304(-3-):F279-F288. >>> Insulin; SC; Mice; 1007D; Animal info (AQP2-Cre, wt).

12466 Ding, Q., Ash, C., Mracek, T., Merry, B., Bing, C. Caloric restriction increases adiponectin expression by adipose tissue and prevents the inhibitory effect of insulin on circulating adiponectin in rats. JOURNAL OF NUTRITIONAL BIOCHEMISTRY 2012; 23(-8-):867-874. >>> Insulin; Saline, isotonic; plasma, rat, stripped; SC; Rat; 2002; Controls received mp w/ vehicle; animal info (male, brown, Norway, 4 mo old).

12296 Nogi, Y., Nagashima, M., Terasaki, M., Nohtomi, K., Watanabe, T., Hirano, T. Glucose-Dependent Insulinotropic Polypeptide Prevents the Progression of Macrophage-Driven Atherosclerosis in Diabetic Apolipoprotein E-Null Mice. PLoS One 2012; 7(-4-):U967-U974. >>> Glucose-dependent insulinotropic polypeptide; Saline; SC; Mice; 1007D; Controls received mp w/ vehicle; animal info (8 wks old, ApoE -/-); functionality of mp verified via plasma drug levels.


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“A continuous infusion paradigm was utilized in both experiments to minimize any potential hemodynamic side effects from bolus NTG administration... Cha et al., Pharm Bioch and Behav 2003;74:551-557.