References on the Administration of Leptin Using ALZET^® Osmotic Pumps

Q0862 Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Serrano,R., Fernandez,A., Ros,M., Carrascosa,J.M., Arribas,C., Martinez,C., Andres,A., Gallardo,N. Regulation of Insulin-Stimulated Glucose Uptake in Rat White Adipose Tissue upon Chronic Central Leptin Infusion: Effects on Adiposity. Endocrinology 2011; 152(-4-):1366-1377. >>> Leptin, rat; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Wistar, 3-mo old); peptides.

Q0662 German,J.P., Thaler,J.P., Wisse,B.E., Oh,I., Sarruf,D.A., Matsen,M.E., Fischer,J.D., Taborsky,GJ Jr, Schwartz,M.W., Morton,G.J. Leptin Activates a Novel CNS Mechanism for Insulin-Independent Normalization of Severe Diabetic Hyperglycemia. Endocrinology 2011; 152(-2-):394-404. >>> Leptin; PBS; CSF/CNS; Rat; 11 days; Controls received mp w/ vehicle; animal info (adult, male, Wistar); peptides; pump implanted following 7-day recovery period after cannula insertion.

Q0884 Mendoza,J., Lopez-Lopez,C., Revel,F.G., Jeanneau,K., Delerue,F., Prinssen,E., Challet,E., Moreau,J.L., Grundschober,C. Dimorphic Effects of Leptin on the Circadian and Hypocretinergic Systems of Mice. Journal of Neuroendocrinology 2011; 23(-1-):28-38. >>> Leptin; NaCl; SC; Mice; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (male, female, C57BL/6, 23 g).

P9975 German,J.P., Wisse,B.E., Thaler,J.P., Oh,I., Sarruf,D.A., Ogimoto,K., Kaiyala,K.J., Fischer,J.D., Matsen,M.E., Taborsky,GJ Jr, Schwartz,M.W., Morton,G.J. Leptin Deficiency Causes Insulin Resistance Induced by Uncontrolled Diabetes. Diabetes 2010; 59(-7-):1626-1634. >>> Leptin; SC; Rat; 2ML4; ; Controls received mp w/ vehicle; animal info (male, Wistar, adult).

Q0513 Gil-Ortega,M., Stucchi,P., Guzman-Ruiz,R., Cano,V., Arribas,S., Gonzalez,M.C., Ruiz-Gayo,M., Fernandez-Alfonso,M.S., Somoza,B. Adaptative Nitric Oxide Overproduction in Perivascular Adipose Tissue during Early Diet-Induced Obesity. Endocrinology 2010; 151(-7-):3299-3306. >>> Leptin; SC; Mice; 2004; 4 weeks; Controls received mp w/ saline; animal info (ob/ob, 4 wks old).

Q0401 Harris,R.B.S. Leptin Responsiveness of Mice Deficient in Corticotrophin-Releasing Hormone Receptor Type 2. Neuroendocrinology 2010; 92(-3-):198-206. >>> Leptin; IP; Mice; 1002; 12 days; Controls received mp w/PBS; animal info (male, female, wt, CRHR2 KO).

Q0121 Hilzendeger,A.M., Goncalves,A.C.D., Plehm,R., Diedrich,A., Gross,V., Pesquero,J.B., Bader,M. Autonomic dysregulation in ob/ob mice is improved by inhibition of angiotensin-converting enzyme. JOURNAL OF MOLECULAR MEDICINE-JMM 2010; 88(-4-):383-390. >>> Leptin; Mice; 28 days; Peptides; animal info (male, C57BL/6J wt, ob/ob, 16 weeks old).

Q0313 Inoue,W., Luheshi,G.N. Acute starvation alters lipopolysaccharide-induced fever in leptin-dependent and -independent mechanisms in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2010; 299(-6-):R1709-R1719. >>> Leptin, recomb. rat; Saline; SC; 1007D; 4 days; Controls received mp w/ vehicle; animal info (adult, female, Sprague-Dawley, 200-225 g).

Q0082 Kaiyala,K.J., Morton,G.J., Leroux,B.G., Ogimoto,K., Wisse,B., Schwartz,M.W. Identification of Body Fat Mass as a Major Determinant of Metabolic Rate in Mice. Diabetes 2010; 59(-7-):1657-1666. >>> Leptin; Sodium bicarbonate; SC; Mice; 1007D; ; Controls received mp w/ vehicle; animal info (ob/ob, wt, C57BL/6, 11 wks old); obesity; diabetes; Lynch coil filled with saline for 24 hour delayed infusion.

Q0085 Knight,Z.A., Hannan,K.S., Greenberg,M.L., Friedman,J.M. Hyperleptinemia Is Required for the Development of Leptin Resistance. PLoS One 2010; 5(-6-):U248-U255. >>> Leptin, recomb.; PBS; SC; Mice; 1004; 2002; 2004; 20 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks, or 12 days; animal info (male, ob/ob C57BL/6J, 4 wks old); functionality of mp verified by plasma leptin levels; "We found, consistent with previous studies, that leptin replacement in ob/ob animals is sufficient to normalize their body weight and other physiologic abnormalities, and furthermore that this normalization can be maintained for 6 months by replacing the pumps monthly." pg. e11377; obesity.

Q0163 Park,S., Ahn,I.S., Kim,D.S. Central infusion of leptin improves insulin resistance and suppresses beta-cell function, but not beta-cell mass, primarily through the sympathetic nervous system in a type 2 diabetic rat model. LIFE SCIENCES 2010; 86(-23-24-):854-862. >>> Leptin; CSF, artificial; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 223 g.); aCSF recipe; diabetes.

Q0093 Pfaffly,J., Michaelides,M., Wang,G.J., Pessin,J.E., Volkow,N.D., Thanos,P.K. Leptin Increases Striatal Dopamine D2 Receptor Binding in Leptin-Deficient Obese (ob/ob) Mice. Synapse 2010; 64(-7-):503-510. >>> Leptin; Saline; SC; Mice; 1002; 8 days; Controls received mp w/ vehicle; animal info (male, ob/ob, 8 wks old); obesity.

P9915 Sikka,G., Yang,R., Reid,S., Benjo,A., Koitabashi,N., Camara,A., Baraban,E., O'Donnell,C.P., Berkowitz,D.E., Barouch,L.A. Leptin is essential in maintaining normal vascular compliance independent of body weight. International Journal of Obesity 2010; 34(-1-):203-206. >>> Leptin; Mice; 2, 4 weeks; Controls received mp w/ PBS; animal info (7-9 mo old, ob/ob, C57BL/6).

P9972 Trevaskis,J.L., Lei,C.L., Koda,J.E., Weyer,C., Parkes,D.G., Roth,J.D. Interaction of Leptin and Amylin in the Long-term Maintenance of Weight Loss in Diet-induced Obese Rats. Obesity 2010; 18(-1-):21-26. >>> Amylin, rat; leptin, murine; Water, sterile; DMSO; SC; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; peptides; long-term study; multiple pumps per animal (2); animal info (male, DIO, 500 g); pumps replaced after 4 weeks; 50% DMSO for amylin; endocrinology.

Q0060 Tschoep,J., Nogueiras,R., Haas-Lockie,S., Kasten,K.R., Castaneda,T.R., Huber,N., Guanciale,K., Perez-Tilve,D., Habegger,K., Ottaway,N., Woods,S.C., Oldfield,B., Clarke,I., Chua,S Jr, Farooqi,I.S., O'Rahilly,S., Caldwell,C.C., Tschoep,M.H. CNS Leptin Action Modulates Immune Response and Survival in Sepsis. Journal of Neuroscience 2010; 30(-17-):6036-6047. >>> Leptin; Saline, isotonic; SC; CSF/CNS; Mice; 1003D; 14 days; Controls received mp w/ vehicle; cyanoacrylate adhesive; ALZET brain infusion kit 3 used; animal info (male, ob/ob, wt, 8-10 wks old); post op. care (buprenorphine).

Q0081 Turek,V.F., Trevaskis,J.L., Levin,B.E., Dunn-Meynell,A.A., Irani,B., Gu,G.B., Wittmer,C., Griffin,P.S., Vu,C., Parkes,D.G., Roth,J.D. Mechanisms of Amylin/Leptin Synergy in Rodent Models. Endocrinology 2010; 151(-1-):143-152. >>> Amylin; leptin; DMSO; water, sterile; SC; Rat; mice; 2004; 2ML4; 28 days; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (lean, male, Sprague Dawley, 426 g., amylin KO, 12 wks old, male, female); one group contained pumps with amylin and leptin; 50% DMSO used; obesity.

P9888 Wang,M.Y., Chen,L., Clark,G.O., Lee,Y., Stevens,R.D., Ilkayeva,O.R., Wenner,B.R., Bain,J.R., Charron,M.J., Newgard,C.B., Unger,R.H. Leptin therapy in insulin-deficient type I diabetes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010; 107(-11-):4813-4819. >>> Leptin; SC; Mice; 2001; 12 days; Controls received mp w/PBS, comparison of insulin pellet v. mp; pumps replaced after 6 days, animal info (5 wks old, NOD).

P9931 Badman,M.K., Kennedy,A.R., Adams,A.C., Pissios,P., Maratos-Flier,E. A very low carbohydrate ketogenic diet improves glucose tolerance in ob/ob mice independently of weight loss. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2009; 297(-5-):E1197-E1204. >>> Leptin; SC; Mice; ; Controls received mp w/ saline; animal info (10wks old, wt, ob/ob).

Q0489 Ceccarini,G., Flavell,R.R., Butelman,E.R., Synan,M., Willnow,T.E., Bar-Dagan,M., Goldsmith,S.J., Kreek,M.J., Kothari,P., Vallabhajosula,S., Muir,T.W., Friedman,J.M. PET Imaging of Leptin Biodistribution and Metabolism in Rodents and Primates. Cell Metabolism 2009; 10(-2-):148-1U7. >>> Leptin, recomb.; leptin, dota-; SC; Mice; 10 days; Animal info (ob/ob, 16 wks old).

Q0447 Collura,L.A., Hoffman,J.B., Wilson,M.E. Administration of human leptin differentially affects parameters of cortisol secretion in socially housed female rhesus monkeys. Endocrine 2009; 36(-3-):530-537. >>> Leptin, recomb. human; SC; Monkey; 28 days; Controls received "no leptin supplementation"; animal info (adult, female, rhesus).

Q0443 da Silva,A.A., do Carmo,J.M., Freeman,J.N., Tallam,L.S., Hall,J.E. A Functional Melanocortin System May Be Required for Chronic CNS-Mediated Antidiabetic and Cardiovascular Actions of Leptin. Diabetes 2009; 58(-8-):1749-1756. >>> Leptin; SHU-9119; CSF/CNS; Rat; 2002; 10, 17 days; Animal info (male, Sprague-Dawley, 325-350 g); pumps replaced after 7 days; multiple pumps per animal (2); guide cannula used; cannula placement verified by angiotensin II dipsogenic effect; diabetes; Leptin and SHU-9119 attached to Y connector.

Q0503 de Chantemele,E.J.B., Muta,K., Mintz,J., Tremblay,M.L., Marrero,M.B., Fulton,D.J., Stepp,D.W. Protein Tyrosine Phosphatase 1B, a Major Regulator of Leptin-Mediated Control of Cardiovascular Function. Circulation 2009; 120(-9-):753-U84. >>> Leptin; SC; Mice; 1007D; 7 days; Balb/c controls received mp w/ leptin; animal info (PTP1B null, Balb/c).

Q0430 French,S.S., Greives,T.J., Zysling,D.A., Chester,E.M., Demas,G.E. Leptin increases maternal investment. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 2009; 276(-1675-):4003-4011. >>> Leptin, recomb. murine; Tris buffer; Hamster; 2002; 14 days; Controls received mp w/ vehicle; animal info (adult, female, Siberian).

P9829 Knight,W.D., Seth,R., Boron,J., Overton,J.M. Short-term physiological hyperleptinemia decreases arterial blood pressure. REGULATORY PEPTIDES 2009; 154(-1-3-):60-68. >>> Leptin, recomb. rat; SC; Rat; 2ML1; 7 days; Controls received mp w/ saline; peptides; animal info (male, Fisher 344 x Brown Norway, Sprague Dawley, 300 g.).

P9347 Kusakabe,T., Tanioka,H., Ebihara,K., Hirata,M., Miyamoto,L., Miyanaga,F., Hige,H., Aotani,D., Fujisawa,T., Masuzaki,H., Hosoda,K., Nakao,K. Beneficial effects of leptin on glycaemic and lipid control in a mouse model of type 2 diabetes with increased adiposity induced by streptozotocin and a high-fat diet. Diabetologia 2009; 52(-4-):675-683. >>> Leptin, recomb. mouse; SC; Mice; 2002; 14 days; Controls received mp w/ saline; animal info (7 wks old, C57BL/6J, male).

P9357 Miyazaki,M., Sampath,H., Liu,X.Q., Flowers,M.T., Chu,K., Dobrzyn,A., Ntambi,J.M. Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. Biochemical and Biophysical Research Communications 2009; 380(-4-):818-822. >>> Leptin, recomb.; SC; Mice; 1002; 4 days; Controls received mp w/ PBS; animal info (Scd1 -/-).

P9551 Motyl,K.J., McCabe,L.R. Leptin Treatment Prevents Type I Diabetic Marrow Adiposity But Not Bone Loss in Mice. Journal of Cellular Physiology 2009; 218(-2-):376-384. >>> Leptin; Saline, sterile; SC; Mice; 2004; ; Controls received mp w/vehicle; post op. care (Carprofen); animal info (14 wks old, BALB/c).

P9982 Singh,A., Wirtz,M., Parker,N., Hogan,M., Strahler,J., Michailidis,G., Schmidt,S., Vidal-Puig,A., Diano,S., Andrews,P., Brand,M.D., Friedman,J. Leptin-mediated changes in hepatic mitochondrial metabolism, structure, and protein levels. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009; 106(-31-):13100-13105. >>> Leptin; SC; Mice; 8 days; Controls received mp w/ sterile PBS; animal info (male, C57BL/6, 8-10 wks old, ob/ob); 0.5 ul/hr rate of infusion; withdrawal.

Q0083 Tang,G.B., Cui,J.G., Wang,D.H. Role of hypoleptinemia during cold adaptation in Brandt's voles (Lasiopodomys brandtii). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-5-):R1293-R1301. >>> Leptin, recomb. murine; PBS; SC; Prairie vole; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, Brandts, 90-120 days old); functionality of mp verified by serum leptin levels.

Q0043 Tirard,J., Gout,J., Sarafian,D., Blondet,A., Vigier,M., Rajas,F., Mithieux,G., Begeot,M., Naville,D. Leptin Infusion and Obesity in Mouse Cause Alterations in the Hypothalamic Melanocortin System. Obesity 2009; 16(--):1763-1769. >>> Leptin, recomb. ovine; PBS; IP; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, C57BL/6J, 4 weeks old).

Q0747 Wang,W.F., Baghdoyan,H.A., Lydic,R. Leptin Replacement Restores Supraspinal Cholinergic Antinociception in Leptin-Deficient Obese Mice. JOURNAL OF PAIN 2009; 10(-8-):836-843. >>> Leptin; Mice; 1007D; 7 days; Animal info (adult, male, B6, obese); functionality of mp verified by leptin ELISA kit; obesity.

P9462 White,C.L., Whittington,A., Barnes,M.J., Wang,Z., Bray,G.A., Morrison,C.D. HF diets increase hypothalamic PTP1B and induce leptin resistance through both leptin-dependent and -independent mechanisms. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2009; 296(-2-):E291-E299. >>> Leptin; Saline; SC; CSF/CNS; Rat; mice; 10, 14 days; Controls received mp w/ vehicle; comparison of IP, ICV injections vs. mp; peptides; post op. care (analgesics); animal info (male, Long Evans, 10 wks old; C57BL/6 wt, ob/ob, 8 wks old).

Q0568 Xu,J., Kirigiti,M.A., Grove,K.L., Smith,M.S. Regulation of Food Intake and Gonadotropin-Releasing Hormone/Luteinizing Hormone during Lactation: Role of Insulin and Leptin. Endocrinology 2009; 150(-9-):4231-4240. >>> Insulin, human; leptin, rat; Saline; SC; Rat; 1003D; 48 hours; Controls received mp w/ vehicle; animal info (d 8-9 post partum, 6 d post ovariectomy); multiple pumps per animal (2); replacement therapy (ovariectomized).

Q0296 Zhang,J.J., Scarpace,P.J. The soluble leptin receptor neutralizes leptin-mediated STAT3 signalling and anorexic responses in vivo. British Journal of Pharmacology 2009; 158(-2-):475-482. >>> Soluble leptin receptor; leptin; CSF, artificial; saline; CSF/CNS; SC; Rat; 2001; 3 weeks; Controls received mp w/ vehicle; animal info (2 mo-old, male, F344 x Brown Norway); ALZET brain infusion kit used; pumps replaced after after 2 weeks; 2 week aCSF delayed infusion; aCSF pump was replaced with SLR pump plus leptin pump after 2 weeks.

P9725 Altarejos,J.Y., Goebel,N., Conkright,M.D., Inotiel,H., Xie,J.X., Arias,C.M., Sawchenko,P.E., Montminy,M. The Creb1 coactivator Crtc1 is required for energy balance and fertility. Nature Medicine 2008; 14(-10-):1112-1117. >>> Leptin; PBS, sterile; SC; Mice; 10 days; Animal info (7-8 wks old, male, Crtc -/-, wt).

P9391 Birsoy,K., Soukas,A., Torrens,J., Ceccarini,G., Montez,J., Maffei,M., Cohen,P., Fayzikhodjaeva,G., Viale,A., Socci,N.D., Friedman,J.M. Cellular program controlling the recovery of adipose tissue mass: An in vivo imaging approach. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-35-):12985-12990. >>> Leptin; PBS; SC; Mice (transgenic); 8 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides; animal info (female, C57BL/6J, BAC Hg, 7-8 wks old).

P9131 Chiba,T., Shinozaki,S., Nakazawa,T., Kawakami,A., Ai,M., Kaneko,E., Kitagawa,M., Kondo,K., Chait,A., Shimokado,K. Leptin deficiency suppresses progression of atherosclerosis in apoE-deficient mice. Atherosclerosis 2008; 196(-1-):68-75. >>> Leptin, recomb. mouse; SC; Mice; 2004; ; Controls received mp w/ saline; peptides; animal info (C57BL/6, ApoE-/-); Research Diets D12108.

P8910 do Carmo,J.M., Hall,J.E., da Silva,A.A. Chronic central leptin infusion restores cardiac sympathetic-vagal balance and baroreflex sensitivity in diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-5-):H1974-H1981. >>> Leptin; CSF/CNS; Rat; 1007D; 7 days; Peptides; animal info (male, Sprague Dawley, 340-360 g.); tygon tubing used; diabetes.

R0247 Fernstrom,J.D., Choi,S. The development of tolerance to drugs that suppress food intake. PHARMACOLOGY&THERAPEUTICS 2008; 117(-1-):105-122. >>> Fenfluramine; leptin; melanotan II; CSF, artificial; PBS; SC; CSF/CNS; Rat; 8 weeks; 7, 28 days; Controls received mp w/ vehicle; dose-response (fig. 5); pumps replaced; tolerance; peptides; animal info (male, obese); endocrinology; review, see p. 108-110.

P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.

P9042 Gutman,R., Hacmon-Keren,R., Choshniak,I., Kronfeld-Schor,N. Effect of food availability and leptin on the physiology and hypothalamic gene expression of the golden spiny mouse: a desert rodent that does not hoard food. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-6-):R2015-R2023. >>> Leptin; SC; Mice; 2002; 23 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; post op. care (Baytril); animal info (golden, spiny, male).

P9569 Hinoi,E., Gao,N., Jung,D.Y., Yadav,V., Yoshizawa,T., Myers,MG Jr, Chua,SC Jr, Kim,J.K., Kaestner,K.H., Karsenty,G. The sympathetic tone mediates leptin's inhibition of insulin secretion by modulating osteocalcin bioactivity. Journal of Cell Biology 2008; 183(-7-):1235-1242. >>> Leptin, human; CSF/CNS (third ventricle); Mice; 7 days; Alzet brain infusion kit 2 used.

P8902 Judge,M.K., Zhang,J., Tuemer,N., Carter,C., Daniels,M.J., Scarpace,P.J. Prolonged hyperphagia with high-fat feeding contributes to exacerbated weight gain in rats with adult-onset obesity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-3-):R773-R780. >>> Leptin, recomb. murine; SC; Rat; 2001; 7 days; Controls received mp w/ saline; dose-response (fig. 8); peptides; animal info (male, F344 x BN); obesity; Research Diets D12492.

P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.

P9682 Martin,A., David,V., Vico,L., Thomas,T. Impaired Energetic Metabolism After Central Leptin Signaling Leads to Massive Appendicular Bone Loss in Hindlimb-Suspended Rats. Journal of Bone and Mineral Research 2008; 23(-12-):2040-2047. >>> Leptin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 14 days; Controls received mp w/ vehicle; animal info (11 wks old, female, Wistar); Plastics One cannula used.

P8800 Park,S., Hong,S.M., Sung,S.R., Jung,H.K. Long-term effects of central leptin and resistin on body weight, insulin resistance, and beta-cell function and mass by the modulation of hypothalamic leptin and insulin signaling. Endocrinology 2008; 149(-2-):445-454. >>> Leptin, recomb. rat; resistin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; replacement therapy (pancreatectomy, 90%); peptides; animal info (male, Sprague Dawley, 220g).

P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).

P9439 Sahu,A. Effects of chronic central leptin infusion on proopiomelanocortin and neurotensin gene expression in the rat hypothalamus. Neuroscience Letters 2008; 440(-2-):125-129. >>> Leptin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 2002; 23 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; pumps replaced after 7 days of mp w/aCSF alone for 16 more days of vehicle or agent; peptides; animal info (male, Sprague Dawley, 250 g.).

P8869 Sennello,J.A., Fayad,R., Pini,M., Gove,M.E., Ponemone,V., Cabay,R.J., Siegmund,B., Dinarello,C.A., Fantuzzi,G. Interleukin-18, together with interleukin-12, induces severe acute pancreatitis in obese but not in nonobese leptin-deficient mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-23-):8085-8090. >>> Leptin; SC; Mice; 1 month; Replacement therapy (leptin deficient); peptides; animal info (ob/ob, slim, female, 6-10 wks old); obesity.

P8866 Terao,S., Yilmaz,G., Stokes,K.Y., Ishikawa,M., Kawase,T., Granger,D.N. Inflammatory and injury responses to ischemic stroke in obese mice. Stroke 2008; 39(-3-):943-950. >>> Leptin, mouse; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma leptin levels; peptides; post op. care (neomycin trisulfate salt hydrate); animal info (young, 5-7 wks old, ob/ob); obesity.

P9173 Trevaskis,J.L., Coffey,T., Cole,R., Lei,C., Wittmer,C., Walsh,B., Weyer,C., Koda,J., Baron,A.D., Parkes,D.G., Roth,J.D. Amylin-Mediated Restoration of Leptin Responsiveness in Diet-Induced Obesity: Magnitude and Mechanisms. Endocrinology 2008; 149(-11-):5679-5687. >>> Leptin, murine; amylin, rat; Water, sterile; DMSO; Rat; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (DIO-prone, Sprague Dawley); 50% DMSO used; Research Diets D1226B.

P8798 Trivedi,P., Yang,R.H., Barouch,L.A. Decreased p110-alpha catalytic activity accompanies increased myocyte apoptosis and cardiac hypertrophy in leptin deficient ob/ob mice. CELL CYCLE 2008; 7(-5-):560-565. >>> Leptin, recomb. mouse; SC; Mice; 1002; 4 weeks; Replacement therapy (leptin-deficient mice); pumps replaced after 14 days; cardiovascular; peptides; animal info (ob/ob, db/db, wt, 2-3 months old, 12-13 months); obesity.

P9174 Trotter-Mayo,R.N., Roberts,M.R. Leptin acts in the periphery to protect thymocytes from glucocorticoid-mediated apoptosis in the absence of weight loss. Endocrinology 2008; 149(-10-):5209-5218. >>> Leptin; IP; CSF/CNS; Mice; 1002; 12 days; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ob/ob, db/db, wt, C57BL/6).

P9025 Unniappan,S., Kieffer,T.J. Leptin extends the anorectic effects of chronic PYY(3-36) administration in ad libitum-fed rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-1-):R51-R58. >>> Peptide YY (3-36), rat; peptide YY (3-36), human; leptin; Saline; SC; Rat; 2001D; 2ML1; 2ML2; 7 days; 24 hours; Controls received mp w/ vehicle; animal info (Fischer 344, 224 g.); 14-day pumps were removed after 7 days, and reimplanted into a new set of animals; "In the coadministration studies, a single pump was used to deliver both PYY (3-36) and leptin." pg. R53; "PYY (3-36) remained biologically active while in the pumps for at least 9 days.".

P9179 Zhang,R., Dhillon,H., Yin,H., Yoshimura,A., Lowell,B.B., Maratos-Flier,E., Flier,J.S. Selective Inactivation of Socs3 in SF1 Neurons Improves Glucose Homeostasis without Affecting Body Weight. Endocrinology 2008; 149(-11-):5654-5661. >>> Leptin, murine; SC; Mice; 1002; 10 days; Controls received mp w/ saline; peptides; animal info (male, C57BL/6, 20 wks old, Socs3 flox/flox, Sf1-Cre, wt).

P9293 Zhang,W., la-Fera,M.A., Hartzell,D.L., Hausman,D., Baile,C.A. Adipose tissue gene expression profiles in ob/ob mice treated with leptin. LIFE SCIENCES 2008; 83(-1-2-):35-42. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response (fig. 1); peptides; animal info (female, ob/ob, 16 wks old).

P8468 Abe,Y., Ono,K., Kawamura,T., Wada,H., Kita,T., Shimatsu,A., Hasegawa,K. Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation. Am.J.Physiol Heart Circ.Physiol 2007/5; 292(-5-):H2387-H2396. >>> Leptin, recomb. mouse; SC; Mice; 2ML4; 4 weeks; Controls received mp w/ sterile PBS; no stress (see pg H2395); cardiovascular; peptides; animal info (C57/BL6, 12 wks old, MI coronary artery ligation); ischemia (cardiac).

P8823 Cariou,B., Bouchaert,E., Abdelkarim,M., Dumont,J., Caron,S., Fruchart,J.C., Burcelin,R., Kuipers,F., Staels,B. FXR-deficiency confers increased susceptibility to torpor. FEBS Letters 2007; 581(-27-):5191-5198. >>> Leptin, recomb. mouse; SC; 1003D; 3 days; Controls received mp w/ sterile saline; functionality of mp verified by plasma leptin levels; peptides; animal info (female, C57BL/6, wt, FXR -/-).

R0241 Crowley,W.R., Ramoz,G., Torto,R., Keefe,K.A., Wang,J.J., Kalra,S.P. Neuroendocrine actions and regulation of hypothalamic neuropeptide Y during lactation. Peptides 2007; 28(-2-):447-452. >>> Leptin, recomb. rat; Insulin; SC; Rat; ; Replacement therapy (suckling induced hypoleptinemia); peptides; animal info (female, lactating); see p. 450; endocrinology.

P8625 Dixit,V.D., Yang,H.W., Sun,Y.X., Weeraratna,A.T., Youm,Y.H., Smith,R.G., Taub,D.D. Ghrelin promotes thymopoiesis during aging. Journal of Clinical Investigation 2007; 117(-10-):2778-2790. >>> Ghrelin; leptin, recomb. mouse; PBS; SC; Mice; 2 weeks; Controls received mp w/ vehicle; peptides; animal info (Balb/c; 2,4,14,20 and 24 months old, femail, C57BL/6, 2,68,18 months old); endocrinology.

P8891 Duan,J., Choi,Y.H., Hartzell,D., la-Fera,M.A., Hamrick,M., Baile,C.A. Effects of subcutaneous leptin injections on hypothalamic gene profiles in lean and ob/ob mice. Obesity 2007; 15(-11-):2624-2633. >>> Leptin, recomb. mouse; SC; Mice; 1002; 14 days; Controls received mp w/ PBS; peptides; animal info (obese, ob/ob, lean).

P8678 Gallardo,N., Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Gomez-Alonso,S., Blanco,P., Carrascosa,J.M., Ros,M., Andres,A. Tissue-specific effects of central leptin on the expression of genes involved in lipid metabolism in liver and white adipose tissue. Endocrinology 2007; 148(-12-):5604-5610. >>> Leptin; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; peptides; animal info (3 months old, Wistar, male); endocrinology.

P8580 Harris,R.B., Mitchell,T.D., Kelso,E.W., Flatt,W.P. Changes in environmental temperature influence leptin responsiveness in low- and high-fat-fed mice. Am J Physiol Regul Integr Comp Physiol 2007/7; 293(-1-):R106-R115. >>> Leptin, recomb. mouse; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; peptides; animal info (male, C57BL/6, 15 weeks old, 25-20 grams).

P8686 Harris,R.B.S., Bartness,T.J., Grill,H.J. Leptin responsiveness in chronically decerebrate rats. Endocrinology 2007; 148(-10-):4623-4633. >>> Leptin; PBS; IP; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum levels; dose-response (fig. 3A); peptides; animal info (Sprague-Dawley, male, 275-300g, chronically decerebrate); endocrinology.

P8700 Hindlet,P., Bado,A., Farinotti,R., Buyse,M. Long-term effect of leptin on H+ -coupled peptide cotransporter 1 activity and expression in vivo: Evidence in leptin-deficient mice. Journal of Pharmacology and Experimental Therapeutics 2007; 323(-1-):192-201. >>> Leptin, recomb.; PBS; SC; Rat; mice; 1007D; 2ML1; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; peptides; animal info (male, Wistar, 250-275g; male, C57BL/6J, wt, ob/ob, 7 wks old).

P8390 Johnston,R.A., Theman,T.A., Terry,R.D., Williams,E.S., Shore,S.A. Pulmonary responses to acute ozone exposure in fasted mice: effect of leptin administration. Journal of Applied Physiology 2007; 102(-1-):149-156. >>> Leptin, recomb. mouse; PBS; HCR; NaOH; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentration; peptides; post op. care (buprenorphine hydrochloride); animal info (male, C57BL/6J, 8 wks old).

P8206 Luque,R.M., Huang,Z.H., Shah,B., Mazzone,T., Kineman,R.D. Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-3-):E891-E899. >>> Leptin, recomb.mouse; Saline; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, C57BL/6J ob/ob or wt, 10 wks old, 45g.); endocrinology.

P8560 Plum,L., Rother,E., Muenzberg,H., Wunderlich,F.T., Morgan,D.A., Hampel,B., Shanabrough,M., Janoschek,R., Koenner,A.C., Alber,J., Suzuki,A., Krone,W., Horvath,T.L., Rahmouni,K., Bruening,J.C. Enhanced leptin-stimulated Pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metabolism 2007; 6(-6-):431-445. >>> Leptin; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Peptides; animal info (male).

P8807 Sato,S., Hanada,R., Kimura,A., Abe,T., Matsumoto,T., Iwasaki,M., Inose,H., Ida,T., Mieda,M., Takeuchi,Y., Fukumoto,S., Fujita,T., Kato,S., Kangawa,K., Kojima,M., Shinomiya,K., Takeda,S. Central control of bone remodeling by neuromedin U. Nature Medicine 2007; 13(-10-):1234-1240. >>> Neuromedin U-23, rat; leptin, human; CSF/CNS; Mice; 28 days; Controls received mp w/ PBS; peptides; animal info (C57BL/6J, wt, LepOb, Nmu -/-, Adrb2 -/-, male, female, 3 months old).

P8586 Scarpace,P.J., Matheny,M., Zhang,Y., Cheng,K.Y., Tumer,N. Leptin antagonist reveals an uncoupling between leptin receptor signal transducer and activator of transcription 3 signaling and metabolic responses with central leptin resistance. J Pharmacol.Exp.Ther. 2007/2; 320(-2-):706-712. >>> Leptin antagonist; NaHCO3; CSF/CNS; 14 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; peptides; animal info (male, F344xBN, 3 months old, 275-375 grams).

P8490 Scherbarth,F., Rozman,J., Klingenspor,M., Brabant,G., Steinlechner,S. Wheel running affects seasonal acclimatization of physiological and morphological traits in the Djungarian hamster (Phodopus sungorus). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 293(-3-):R1368-R1375. >>> Leptin, recomb. mouse; HCL; SC; Hamster; 1007D; 14 days; Pumps replaced every seven days; peptides; animal info (5-7 month old, M&F).

P8599 Stocker,C.J., Wargent,E., O'Dowd,J., Cornick,C., Speakman,J.R., Arch,J.R.S., Cawthorne,M.A. Prevention of diet-induced obesity and impaired glucose tolerance in rats following administration of leptin to their mothers. American Journal of Physiology- Regulatory, Integrative and Comparative Physiology 2007; 292(-5-):R1810-R1818. >>> Leptin, rat; Saline, physiological; SC; Rat (pregnant); 28 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin concentration; teratology; peptides; animal info (female, Wistar, 200-225 grams, gestational day 14).

P8495 Turner,S.M., Roy,S., Sul,H.S., Neese,R.A., Murphy,E.J., Samandi,W., Roohk,D.J., Hellerstein,M.K. Dissociation between adipose tissue fluxes and lipogenic gene expression in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-4-):E1101-E1109. >>> Leptin, recomb. murine; SC; Mice; 28 days; Leptin plasma levels taken; peptides; obesity; animal info (6-7 weeks old, female, C57BL/6J); endocrinology.

P8351 Verlaeten,O., Casery,C., Cavagna,S., Navilie,D., Giraudon,P., Belin,M.F., Begeot,M., Bernard,A. Identification of Urop11, a novel leptin-modulated gene that is upregulated in the hypothalamus of mice with virus-induced obesity. Journal of Molecular Endocrinology 2007; 38(-1-2-):3-17. >>> Leptin; PBS; SC; Mice; 1 week; Peptides; animal info (male, C57BL/6J, control or HFD obese, CDV infected).

P8040 Zhang,J., Matheny,M.K., Tuemer,N., Mitchell,M.K., Scarpace,P.J. Leptin antagonist reveals that the normalization of caloric intake and the thermic effect of food after high-fat feeding are leptin dependent. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 292(-2-):R868-R874. >>> Leptin; leptin antagonist; Sodium bicarbonate; CSF/CNS; 2001; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; peptides; animal info (male, Fischer, Brown Norway); dental cement used to secure cannula.

P7685 Alon,T., Friedman,J.M. Late-onset leanness in mice with targeted ablation of melanin concentrating hormone neurons. Journal of Neuroscience 2006; 26(-2-):389-397. >>> Leptin; PBS; Mice; 2002; 9 days; Peptides; animal info (C57BL/6).

P7600 Barouch,L.A., Gao,D.Q., Chen,L., Miller,K.L., Xu,W.H., Phan,A.C., Kittleson,M.M., Minhas,K.M., Berkowitz,D.E., Wei,C.M., Hare,J.M. Cardiac myocyte apoptosis is associated with increased DNA damage and decreased survival in murine models of obesity. Circulation Research 2006; 98(-1-):119-124. >>> Leptin, recomb. mouse; SC; Mice; 1002; 4 weeks; Pumps replaced after 14 days; cardiovascular; peptides; animal info (6-8 month old); obesity.

P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).

P7895 Chen,K., Li,F.H., Li,J., Cai,H.B., Strom,S., Bisello,A., Kelley,D.E., Friedman-Einat,M., Skibinski,G.A., McCrory,M.A., Szalai,A.J., Zhao,A.Z. Induction of leptin resistance through direct interaction of C-reactive protein with leptin. Nature Medicine 2006; 12(-4-):425-432. >>> Leptin, recomb. human; C-reactive protein, human; Saline; SC; Mice; mice (transgenic); 6, 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum concentrations; dose-response (fig.5); peptides; MRI; multiple pumps per animal (2); animal info (wt or ob/ob, 8 wk old, human CRPtg.).

P9011 Covey,S.D., Wideman,R.D., McDonald,C., Unniappan,S., Huynh,F., Asadi,A., Speck,M., Webber,T., Chua,S.C., Kieffer,T.J. The pancreatic beta cell is a key site for mediating the effects of leptin on glucose homeostasis. Cell Metabolism 2006; 4(-4-):291-302. >>> Leptin; PBS; SC; Mice; mice (transgenic); 2001D; 12 hours; Controls received mp w/ saline; peptides; animal info (C57BL/6-TgN (R/Pcre) 25 Mgn, Lepr flox/flox, 10 wks old).

P7888 da Silva,A.A., Tallam,L.S., Liu,J.K., Hall,J.E. Chronic antidiabetic and cardiovascular actions of leptin: role of CNS and increased adrenergic activity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 291(-5-):R1275-R1282. >>> Leptin; Saline; CSF/CNS; Rat; 2002; 10 days; Controls received IV saline; functionality of mp verified by plasma leptin concentrations; comparison of IV infusion vs. mp; cardiovascular; peptides; animal info (male, Sprague-Dawley, 275-325g.).

P7451 Fekete,C., Singru,P.S., Sanchez,E., Sarkar,S., Christoffolete,M.A., Riberio,R.S., Rand,W.M., Emerson,C.H., Bianco,A.C., Lechan,R.M. Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. Endocrinology 2006; 147(-1-):520-529. >>> Leptin, recomb. mouse; insulin; glucose; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by agent levels in CSF+ serum; peptides; post op. care (bacitracin ointment); animal info (male Sprague-Dawley, 200-250 g); cannula placement confirmed by light microscope.

P8088 Fulton,S., Pissios,P., Manchon,R.P., Stiles,L., Frank,L., Pothos,E.N., Maratos-Flier,E., Flier,J.S. Leptin regulation of the mesoaccumbens dopamine pathway. Neuron 2006; 51(-6-):811-822. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1003D; 1002; 3, 11, 14 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); peptides; animal info (male, C57BL/6J wt, ob/ob or Lepr (db/db); 4-10 wks old); mp primed 4-6 hrs. at 37 celcius.

P8141 Huang,Q.L., Timofeeva,E., Richard,D. Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. Journal of Endocrinology 2006; 191(-1-):179-188. >>> Leptin, recomb. murine; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ PBS; peptides; animal info (male, Wistar, 270-290g.); tissue perfusion (ventricle).

P8941 Komatsu,T., Chiba,T., Yamaza,H., To,K., Toyama,H., Higami,Y., Shimokawa,I. Effect of leptin on hypothalamic gene expression in calorie-restricted rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 2006; 61(-9-):890-898. >>> Leptin, recomb.; PBS; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Fischer 344, 34 wks old).

P7811 Krol,E., Duncan,J.S., Redman,P., Morgan,P.J., Mercer,J.G., Speakman,J.R. Photoperiod regulates leptin sensitivity in field voles, Microtus agrestis. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 2006; 176(-2-):153-163. >>> Leptin, recomb. mouse; PBS; SC; Vole; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides; animal info (male, field vole (microtus agrestis), 20-23 wk old, 26g.); "the osmotic pumps were effective in delivering biologically active hormone to the animals." (pg. 160).

P8995 Leclercq,I.A., Vansteenberghe,M., Lebrun,V.B., VanHul,N.K., barca-Quinones,J., Sempoux,C.L., Picard,C., Staerkel,P., Horsmans,Y.L. Defective hepatic regeneration after partial hepatectomy in leptin-deficient mice is not rescued by exogenous leptin. LABORATORY INVESTIGATION 2006; 86(-11-):1161-1171. >>> Leptin, recomb. mouse; SC; Mice; 1003D; 44 hours; Controls received no treatment; functionality of mp verified by serum leptin concentrations; replacement therapy (partial hepatectomy); comparison of IP injections vs. mp; peptides; animal info (female, ob/ob, lean).

P7710 Lohmus,M., Sundstroem,L.F., Silverin,B. Chronic administration of leptin in Asian Blue Quail. JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-COMPARATIVE EXPERIMENTAL BIOLOGY 2006; 305A(-1-):13-22. >>> Leptin, recomb. chicken; PBS; SC; Bird (quail); 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume at 3 time points; peptides; animal info (male, asian blue quail, 50 g, young).

P8915 Lujan,M.E., Krzemien,A.A., Reid,R.L., Van Vugt,D.A. Effect of leptin administration on ovulation in food-restricted rhesus monkeys. Neuroendocrinology 2006; 84(-2-):103-114. >>> Leptin, recomb. human; Water, sterile; CSF/CNS; Monkey; 2002; 32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 wks; stress/adverse reaction: (see pg. 104) 1 animal removed from study due to CNS infection at 28 weeks; peptides; animal info (female, Rhesus, 6-10 yrs. old, 5-12 kg.); endocrinology.

P8262 Nogueiras,R., Tovar,S., Mitchell,S.E., Barrett,P., Rayner,D.V., Dieguez,C., Williams,L.M. Negative energy balance and leptin regulate neuromedin-U expression in the rat pars tuberalis. Journal of Endocrinology 2006; 190(-2-):545-553. >>> Leptin, recomb. human; Saline; CSF/CNS; Rat; 2ML2; 7 days; 24, 48 hours; Controls received mp w/ vehicle; dose-response (fig. 6); peptides; animal info (male, Sprague-Dawley, 8-10 wks old, 300g.); cannula placement confirmed by methylene blue staining; endocrinology.

P7907 Wagoner,B., Hausman,D.B., Harris,R.B.S. Direct and indirect effects of leptin on preadipocyte proliferation and differentiation. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 290(-6-):R1557-R1564. >>> Leptin, recomb rat; PBS; IP; Rat; 1007D; 5 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentrations; no stress (see pg. R1559); peptides; animal info (male, Sprague-Dawley, 320-340 g).

P7518 Wilsey,J., Matheny,M.K., Scarpace,P.J. Oral vanadium enhances the catabolic effects of central leptin in young adult rats. Endocrinology 2006; 147(-1-):493-501. >>> Leptin; CSF, artificial; CSF/CNS; Rat; 2004; 21, 28 days; Controls received mp w/ vehicle; dose-response (fig. 7); ALZET brain infusion kit used; peptides; animal info (F344, brown Norway, male, 3 months old, 357-370 g); mp primed 36 hours in 37 C sterile saline.