References on the Administration of Leptin Using ALZET® Osmotic Pumps
R0247 Fernstrom,J.D., Choi,S. The development of tolerance to drugs that suppress food intake. PHARMACOLOGY&THERAPEUTICS 2008; 117(-1-):105-122. >>> Fenfluramine; leptin; melanotan II; CSF, artificial; PBS; SC; CSF/CNS; Rat; 8 weeks; 7, 28 days; Controls received mp w/ vehicle; dose-response (fig. 5); pumps replaced; tolerance; peptides; animal info (male, obese); endocrinology; review, see p. 108-110.
R0241 Crowley,W.R., Ramoz,G., Torto,R., Keefe,K.A., Wang,J.J., Kalra,S.P. Neuroendocrine actions and regulation of hypothalamic neuropeptide Y during lactation. Peptides 2007; 28(-2-):447-452. >>> Leptin, recomb. rat; Insulin; SC; Rat; ; Replacement therapy (suckling induced hypoleptinemia); peptides; animal info (female, lactating); see p. 450; endocrinology.
R0212 Sahu,A. Leptin signaling in the hypothalamus: emphasis on energy homeostasis and leptin resistance. Frontiers in Neuroendocrinology 2003; 24(-4-):225-253. >>> Leptin, recomb., mouse; CSF, artificial; PBS; CSF/CNS; Rat; mice; 4 weeks; Controls received mp w/ vehicle; p. 237-238.
P9179 Zhang,R., Dhillon,H., Yin,H., Yoshimura,A., Lowell,B.B., Maratos-Flier,E., Flier,J.S. Selective Inactivation of Socs3 in SF1 Neurons Improves Glucose Homeostasis without Affecting Body Weight. Endocrinology 2008; 149(-11-):5654-5661. >>> Leptin, murine; SC; Mice; 1002; 10 days; Controls received mp w/ saline; peptides; animal info (male, C57BL/6, 20 wks old, Socs3 flox/flox, Sf1-Cre, wt).
P9174 Trotter-Mayo,R.N., Roberts,M.R. Leptin acts in the periphery to protect thymocytes from glucocorticoid-mediated apoptosis in the absence of weight loss. Endocrinology 2008; 149(-10-):5209-5218. >>> Leptin; IP; CSF/CNS; Mice; 1002; 12 days; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ob/ob, db/db, wt, C57BL/6).
P9173 Trevaskis,J.L., Coffey,T., Cole,R., Lei,C., Wittmer,C., Walsh,B., Weyer,C., Koda,J., Baron,A.D., Parkes,D.G., Roth,J.D. Amylin-Mediated Restoration of Leptin Responsiveness in Diet-Induced Obesity: Magnitude and Mechanisms. Endocrinology 2008; 149(-11-):5679-5687. >>> Leptin, murine; amylin, rat; Water, sterile; DMSO; Rat; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (DIO-prone, Sprague Dawley); 50% DMSO used; Research Diets D1226B.
P9131 Chiba,T., Shinozaki,S., Nakazawa,T., Kawakami,A., Ai,M., Kaneko,E., Kitagawa,M., Kondo,K., Chait,A., Shimokado,K. Leptin deficiency suppresses progression of atherosclerosis in apoE-deficient mice. Atherosclerosis 2008; 196(-1-):68-75. >>> Leptin, recomb. mouse; SC; Mice; 2004; ; Controls received mp w/ saline; peptides; animal info (C57BL/6, ApoE-/-); Research Diets D12108.
P9042 Gutman,R., Hacmon-Keren,R., Choshniak,I., Kronfeld-Schor,N. Effect of food availability and leptin on the physiology and hypothalamic gene expression of the golden spiny mouse: a desert rodent that does not hoard food. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-6-):R2015-R2023. >>> Leptin; SC; Mice; 2002; 23 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; post op. care (Baytril); animal info (golden, spiny, male).
P9025 Unniappan,S., Kieffer,T.J. Leptin extends the anorectic effects of chronic PYY(3-36) administration in ad libitum-fed rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-1-):R51-R58. >>> Peptide YY (3-36), rat; peptide YY (3-36), human; leptin; Saline; SC; Rat; 2001D; 2ML1; 2ML2; 7 days; 24 hours; Controls received mp w/ vehicle; animal info (Fischer 344, 224 g.); 14-day pumps were removed after 7 days, and reimplanted into a new set of animals; "In the coadministration studies, a single pump was used to deliver both PYY (3-36) and leptin." pg. R53; "PYY (3-36) remained biologically active while in the pumps for at least 9 days.".
P9011 Covey,S.D., Wideman,R.D., McDonald,C., Unniappan,S., Huynh,F., Asadi,A., Speck,M., Webber,T., Chua,S.C., Kieffer,T.J. The pancreatic beta cell is a key site for mediating the effects of leptin on glucose homeostasis. Cell Metabolism 2006; 4(-4-):291-302. >>> Leptin; PBS; SC; Mice; mice (transgenic); 2001D; 12 hours; Controls received mp w/ saline; peptides; animal info (C57BL/6-TgN (R/Pcre) 25 Mgn, Lepr flox/flox, 10 wks old).
P8995 Leclercq,I.A., Vansteenberghe,M., Lebrun,V.B., VanHul,N.K., barca-Quinones,J., Sempoux,C.L., Picard,C., Staerkel,P., Horsmans,Y.L. Defective hepatic regeneration after partial hepatectomy in leptin-deficient mice is not rescued by exogenous leptin. LABORATORY INVESTIGATION 2006; 86(-11-):1161-1171. >>> Leptin, recomb. mouse; SC; Mice; 1003D; 44 hours; Controls received no treatment; functionality of mp verified by serum leptin concentrations; replacement therapy (partial hepatectomy); comparison of IP injections vs. mp; peptides; animal info (female, ob/ob, lean).
P8941 Komatsu,T., Chiba,T., Yamaza,H., To,K., Toyama,H., Higami,Y., Shimokawa,I. Effect of leptin on hypothalamic gene expression in calorie-restricted rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 2006; 61(-9-):890-898. >>> Leptin, recomb.; PBS; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Fischer 344, 34 wks old).
P8915 Lujan,M.E., Krzemien,A.A., Reid,R.L., Van Vugt,D.A. Effect of leptin administration on ovulation in food-restricted rhesus monkeys. Neuroendocrinology 2006; 84(-2-):103-114. >>> Leptin, recomb. human; Water, sterile; CSF/CNS; Monkey; 2002; 32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 wks; stress/adverse reaction: (see pg. 104) 1 animal removed from study due to CNS infection at 28 weeks; peptides; animal info (female, Rhesus, 6-10 yrs. old, 5-12 kg.); endocrinology.
P8910 do Carmo,J.M., Hall,J.E., da Silva,A.A. Chronic central leptin infusion restores cardiac sympathetic-vagal balance and baroreflex sensitivity in diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-5-):H1974-H1981. >>> Leptin; CSF/CNS; Rat; 1007D; 7 days; Peptides; animal info (male, Sprague Dawley, 340-360 g.); tygon tubing used; diabetes.
P8902 Judge,M.K., Zhang,J., Tuemer,N., Carter,C., Daniels,M.J., Scarpace,P.J. Prolonged hyperphagia with high-fat feeding contributes to exacerbated weight gain in rats with adult-onset obesity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-3-):R773-R780. >>> Leptin, recomb. murine; SC; Rat; 2001; 7 days; Controls received mp w/ saline; dose-response (fig. 8); peptides; animal info (male, F344 x BN); obesity.
P8891 Duan,J., Choi,Y.H., Hartzell,D., la-Fera,M.A., Hamrick,M., Baile,C.A. Effects of subcutaneous leptin injections on hypothalamic gene profiles in lean and ob/ob mice. Obesity 2007; 15(-11-):2624-2633. >>> Leptin, recomb. mouse; SC; Mice; 1002; 14 days; Controls received mp w/ PBS; peptides; animal info (obese, ob/ob, lean).
P8869 Sennello,J.A., Fayad,R., Pini,M., Gove,M.E., Ponemone,V., Cabay,R.J., Siegmund,B., Dinarello,C.A., Fantuzzi,G. Interleukin-18, together with interleukin-12, induces severe acute pancreatitis in obese but not in nonobese leptin-deficient mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-23-):8085-8090. >>> Leptin; SC; Mice; 1 month; Replacement therapy (leptin deficient); peptides; animal info (ob/ob, slim, female, 6-10 wks old); obesity.
P8866 Terao,S., Yilmaz,G., Stokes,K.Y., Ishikawa,M., Kawase,T., Granger,D.N. Inflammatory and injury responses to ischemic stroke in obese mice. Stroke 2008; 39(-3-):943-950. >>> Leptin, mouse; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma leptin levels; peptides; post op. care (neomycin trisulfate salt hydrate); animal info (young, 5-7 wks old, ob/ob); obesity.
P8823 Cariou,B., Bouchaert,E., Abdelkarim,M., Dumont,J., Caron,S., Fruchart,J.C., Burcelin,R., Kuipers,F., Staels,B. FXR-deficiency confers increased susceptibility to torpor. FEBS Letters 2007; 581(-27-):5191-5198. >>> Leptin, recomb. mouse; SC; 1003D; 3 days; Controls received mp w/ sterile saline; functionality of mp verified by plasma leptin levels; peptides; animal info (female, C57BL/6, wt, FXR -/-).
P8807 Sato,S., Hanada,R., Kimura,A., Abe,T., Matsumoto,T., Iwasaki,M., Inose,H., Ida,T., Mieda,M., Takeuchi,Y., Fukumoto,S., Fujita,T., Kato,S., Kangawa,K., Kojima,M., Shinomiya,K., Takeda,S. Central control of bone remodeling by neuromedin U. Nature Medicine 2007; 13(-10-):1234-1240. >>> Neuromedin U-23, rat; leptin, human; CSF/CNS; Mice; 28 days; Controls received mp w/ PBS; peptides; animal info (C57BL/6J, wt, LepOb, Nmu -/-, Adrb2 -/-, male, female, 3 months old).
P8800 Park,S., Hong,S.M., Sung,S.R., Jung,H.K. Long-term effects of central leptin and resistin on body weight, insulin resistance, and beta-cell function and mass by the modulation of hypothalamic leptin and insulin signaling. Endocrinology 2008; 149(-2-):445-454. >>> Leptin, recomb. rat; resistin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; replacement therapy (pancreatectomy, 90%); peptides; animal info (male, Sprague Dawley, 220g).
P8798 Trivedi,P., Yang,R.H., Barouch,L.A. Decreased p110-alpha catalytic activity accompanies increased myocyte apoptosis and cardiac hypertrophy in leptin deficient ob/ob mice. CELL CYCLE 2008; 7(-5-):560-565. >>> Leptin, recomb. mouse; SC; Mice; 1002; 4 weeks; Replacement therapy (leptin-deficient mice); pumps replaced after 14 days; cardiovascular; peptides; animal info (ob/ob, db/db, wt, 2-3 months old, 12-13 months); obesity.
P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.
P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).
P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.
P8700 Hindlet,P., Bado,A., Farinotti,R., Buyse,M. Long-term effect of leptin on H+ -coupled peptide cotransporter 1 activity and expression in vivo: Evidence in leptin-deficient mice. Journal of Pharmacology and Experimental Therapeutics 2007; 323(-1-):192-201. >>> Leptin, recomb.; PBS; SC; Rat; mice; 1007D; 2ML1; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; peptides; animal info (male, Wistar, 250-275g; male, C57BL/6J, wt, ob/ob, 7 wks old).
P8686 Harris,R.B.S., Bartness,T.J., Grill,H.J. Leptin responsiveness in chronically decerebrate rats. Endocrinology 2007; 148(-10-):4623-4633. >>> Leptin; PBS; IP; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum levels; dose-response (fig. 3A); peptides; animal info (Sprague-Dawley, male, 275-300g, chronically decerebrate); endocrinology.
P8678 Gallardo,N., Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Gomez-Alonso,S., Blanco,P., Carrascosa,J.M., Ros,M., Andres,A. Tissue-specific effects of central leptin on the expression of genes involved in lipid metabolism in liver and white adipose tissue. Endocrinology 2007; 148(-12-):5604-5610. >>> Leptin; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; peptides; animal info (3 months old, Wistar, male); endocrinology.
P8625 Dixit,V.D., Yang,H.W., Sun,Y.X., Weeraratna,A.T., Youm,Y.H., Smith,R.G., Taub,D.D. Ghrelin promotes thymopoiesis during aging. Journal of Clinical Investigation 2007; 117(-10-):2778-2790. >>> Ghrelin; leptin, recomb. mouse; PBS; SC; Mice; 2 weeks; Controls received mp w/ vehicle; peptides; animal info (Balb/c; 2,4,14,20 and 24 months old, femail, C57BL/6, 2,68,18 months old); endocrinology.
P8599 Stocker,C.J., Wargent,E., O'Dowd,J., Cornick,C., Speakman,J.R., Arch,J.R.S., Cawthorne,M.A. Prevention of diet-induced obesity and impaired glucose tolerance in rats following administration of leptin to their mothers. American Journal of Physiology- Regulatory, Integrative and Comparative Physiology 2007; 292(-5-):R1810-R1818. >>> Leptin, rat; Saline, physiological; SC; Rat (pregnant); 28 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin concentration; teratology; peptides; animal info (female, Wistar, 200-225 grams, gestational day 14).
P8588 Tannenbaum,G.S., Gurd,W., Lapointe,M. Leptin Is a Potent Stimulator of Spontaneous Pulsatile Growth Hormone (GH) Secretion and the GH Response to GH-Releasing Hormone. Endocrinology 1998; 139(-9-):3871-3875. >>> Leptin, recomb. mouse; PBS; CSF/CNS; Rat; 2001; 6-7 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Sprague-Dawley, 225-300 grams); cannula placement verified with methylene blue dye.
P8586 Scarpace,P.J., Matheny,M., Zhang,Y., Cheng,K.Y., Tumer,N. Leptin antagonist reveals an uncoupling between leptin receptor signal transducer and activator of transcription 3 signaling and metabolic responses with central leptin resistance. J Pharmacol.Exp.Ther. 2007/2; 320(-2-):706-712. >>> Leptin antagonist; NaHCO3; CSF/CNS; 14 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; peptides; animal info (male, F344xBN, 3 months old, 275-375 grams).
P8580 Harris,R.B., Mitchell,T.D., Kelso,E.W., Flatt,W.P. Changes in environmental temperature influence leptin responsiveness in low- and high-fat-fed mice. Am J Physiol Regul Integr Comp Physiol 2007/7; 293(-1-):R106-R115. >>> Leptin, recomb. mouse; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; peptides; animal info (male, C57BL/6, 15 weeks old, 25-20 grams).
P8572 Surwit,R.S., Edwards,C.L., Murthy,S., Petro,A.E. Transient effects of long-term leptin supplementation in the prevention of diet-induced obesity in mice. Diabetes 2000; 49(-7-):1203-1208. >>> Leptin, recomb. mouse; SC; Mice; 2004; 12 weeks; Controls received mp w/ PBS; functionality of mp verified by plasma levels and residual volume; long-term study; pumps replaced every 4 weeks; no stress (see pg. 1205); peptides; animal info (male, C57BL/6J, B6, A/J, 20 grams); obesity.
P8571 Ioffe,E., Moon,B., Connolly,E., Friedman,J.M. Abnormal regulation of the leptin gene in the pathogenesis of obesity. Proc Natl Acad Sci US A 1998; 95(-20-):11852-11857. >>> Leptin, recomb. murine; SC; Mice (transgenic); 28 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; animal info (male, C57BL/6J ob/ob Tg).
P8560 Plum,L., Rother,E., Muenzberg,H., Wunderlich,F.T., Morgan,D.A., Hampel,B., Shanabrough,M., Janoschek,R., Koenner,A.C., Alber,J., Suzuki,A., Krone,W., Horvath,T.L., Rahmouni,K., Bruening,J.C. Enhanced leptin-stimulated Pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metabolism 2007; 6(-6-):431-445. >>> Leptin; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Peptides; animal info (male).
P8495 Turner,S.M., Roy,S., Sul,H.S., Neese,R.A., Murphy,E.J., Samandi,W., Roohk,D.J., Hellerstein,M.K. Dissociation between adipose tissue fluxes and lipogenic gene expression in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-4-):E1101-E1109. >>> Leptin, recomb. murine; SC; Mice; 28 days; Leptin plasma levels taken; peptides; obesity; animal info (6-7 weeks old, female, C57BL/6J); endocrinology.
P8490 Scherbarth,F., Rozman,J., Klingenspor,M., Brabant,G., Steinlechner,S. Wheel running affects seasonal acclimatization of physiological and morphological traits in the Djungarian hamster (Phodopus sungorus). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 293(-3-):R1368-R1375. >>> Leptin, recomb. mouse; HCL; SC; Hamster; 1007D; 14 days; Pumps replaced every seven days; peptides; animal info (5-7 month old, M&F).
P8473 Harris,R.B.S., Mitchell,T.D., Hebert,S. Leptin-Induced Changes in Body Composition in High Fat-Fed Mice1. EXPERIMENTAL BIOLOGY AND MEDICINE 2003; 228(-1-):24-32. >>> Leptin, recomb. mouse; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; functionality of mp verified by serum leptin concentrations; comparison of IP injections vs. mp; peptides; animal info (female, C57BL/6J, 8 weeks old); obesity.
P8471 Fewlass,D.C., Noboa,K., Pi-Sunyer,F.X., Johnston,J.M., Yan,S.D., Tezapsidis,N. Obesity-related leptin regulates Alzheimer's A beta. FASEB J. 2004/12; 18(-15-):1870-1878. >>> Leptin, recomb. mouse; PBS; SC; Mice (transgenic); 2004; 4,8; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks; peptides; post op. care (lidocaine); neurodegenerative(Alzheimer's disease); animal info (male, female, Tg2576 APPswe, C57BL/6 wt, 10 wks old, 32 wks old); obesity.
P8468 Abe,Y., Ono,K., Kawamura,T., Wada,H., Kita,T., Shimatsu,A., Hasegawa,K. Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation. Am.J.Physiol Heart Circ.Physiol 2007/5; 292(-5-):H2387-H2396. >>> Leptin, recomb. mouse; SC; Mice; 2ML4; 4 weeks; Controls received mp w/ sterile PBS; no stress (see pg H2395); cardiovascular; peptides; animal info (C57/BL6, 12 wks old, MI coronary artery ligation); ischemia (cardiac).
P8390 Johnston,R.A., Theman,T.A., Terry,R.D., Williams,E.S., Shore,S.A. Pulmonary responses to acute ozone exposure in fasted mice: effect of leptin administration. Journal of Applied Physiology 2007; 102(-1-):149-156. >>> Leptin, recomb. mouse; PBS; HCR; NaOH; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentration; peptides; post op. care (buprenorphine hydrochloride); animal info (male, C57BL/6J, 8 wks old).
P8351 Verlaeten,O., Casery,C., Cavagna,S., Navilie,D., Giraudon,P., Belin,M.F., Begeot,M., Bernard,A. Identification of Urop11, a novel leptin-modulated gene that is upregulated in the hypothalamus of mice with virus-induced obesity. Journal of Molecular Endocrinology 2007; 38(-1-2-):3-17. >>> Leptin; PBS; SC; Mice; 1 week; Peptides; animal info (male, C57BL/6J, control or HFD obese, CDV infected).
P8324 Shimomura,I., Hammer,R.E., Ikemoto,S., Brown,M.S., Goldstein,J.L. Leptin reverses insulin resistance and diabetes mellitus in mice with congenital lipodystrophy. Nature 1999/9/2; 401(-6748-):73-76. >>> Leptin, recomb. mouse; PBS, Dulbecco's; SC; Mice (transgenic); 1002; 12 days; Controls received mp w/ vehicle; peptides; animal info (C57BL/6J, ob/ob, male, female, 14-16 weeks old).
P8262 Nogueiras,R., Tovar,S., Mitchell,S.E., Barrett,P., Rayner,D.V., Dieguez,C., Williams,L.M. Negative energy balance and leptin regulate neuromedin-U expression in the rat pars tuberalis. Journal of Endocrinology 2006; 190(-2-):545-553. >>> Leptin, recomb. human; Saline; CSF/CNS; Rat; 2ML2; 7 days; 24, 48 hours; Controls received mp w/ vehicle; dose-response (fig. 6); peptides; animal info (male, Sprague-Dawley, 8-10 wks old, 300g.); cannula placement confirmed by methylene blue staining; endocrinology.
P8222 Gavrilova,O., Marcus-Samuels,B., Leon,L.R., Vinson,C., Reitman,M.L. Leptin and diabetes in lipoatrophic mice. Nature 2000; 403(-6772-):850-. >>> Leptin; Mice (transgenic); 4 weeks; 6 days; Controls received mp w/ saline; dose-response (fig. 1); peptides; animal info (male, 7, 13 weeks old); mouse model of lipodystrophy.
P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).
P8206 Luque,R.M., Huang,Z.H., Shah,B., Mazzone,T., Kineman,R.D. Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-3-):E891-E899. >>> Leptin, recomb.mouse; Saline; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, C57BL/6J ob/ob or wt, 10 wks old, 45g.); endocrinology.
P8186 la-Fera,M.A., Choi,Y.H., Hartzell,D.L., Duan,J.H., Hamrick,M., Baile,C.A. Sensitivity of ob/ob mice to leptin-induced adipose tissue apoptosis. Obesity Research 2005; 13(-9-):1540-1547. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response (fig 1); peptides; animal info (female, ob/ob or wt, 15 wk old, 60-65 grams, 20-25 grams).
P8155 Burcelin,R., Kamohara,S. Acute stimulation of glucose metabolism in mice by leptin treatment. Nature 1997/1/1; 389(--):374-377. >>> Leptin; CSF, artificial; CSF/CNS (third ventricle); Mice; 10 days; Controls received mp w/ PBS; comparison of acute IV infusion vs. mp; CSF pumps replaced with leptin mp after 7 days; peptides; animal info (C57BL/6J +/+ lean, 25 grams); tissue perfusion (ventricle).
P8141 Huang,Q.L., Timofeeva,E., Richard,D. Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. Journal of Endocrinology 2006; 191(-1-):179-188. >>> Leptin, recomb. murine; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ PBS; peptides; animal info (male, Wistar, 270-290g.); tissue perfusion (ventricle).
P8088 Fulton,S., Pissios,P., Manchon,R.P., Stiles,L., Frank,L., Pothos,E.N., Maratos-Flier,E., Flier,J.S. Leptin regulation of the mesoaccumbens dopamine pathway. Neuron 2006; 51(-6-):811-822. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1003D; 1002; 3, 11, 14 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); peptides; animal info (male, C57BL/6J wt, ob/ob or Lepr (db/db); 4-10 wks old); mp primed 4-6 hrs. at 37 celcius.
P8040 Zhang,J., Matheny,M.K., Tuemer,N., Mitchell,M.K., Scarpace,P.J. Leptin antagonist reveals that the normalization of caloric intake and the thermic effect of food after high-fat feeding are leptin dependent. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 292(-2-):R868-R874. >>> Leptin; leptin antagonist; Sodium bicarbonate; CSF/CNS; 2001; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; peptides; animal info (male, Fischer, Brown Norway); dental cement used to secure cannula.
P8014 izawa-Abe,M., Ogawa,Y., Masuzaki,H., Ebihara,K., Satoh,N., Iwai,H., Matsuoka,N., Hayashi,T., Hosoda,K., Inoue,G., Yoshimasa,Y., Nakao,K. Pathophysiological role of leptin in obesity-related hypertension. J.Clin.Invest 2000/5; 105(-9-):1243-1252. >>> Leptin, recomb. mouse; SC; Mice; 1003D; 2002; 3 days; Controls received mp w/ vehicle; blood pressure taken; leptin plasma levels; cardiovascular; peptides; animal info (10 weeks old, male, transgenic, leptin defficient, C57BL/6J).
P7907 Wagoner,B., Hausman,D.B., Harris,R.B.S. Direct and indirect effects of leptin on preadipocyte proliferation and differentiation. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 290(-6-):R1557-R1564. >>> Leptin, recomb rat; PBS; IP; Rat; 1007D; 5 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentrations; no stress (see pg. R1559); peptides; animal info (male, Sprague-Dawley, 320-340 g).
P7895 Chen,K., Li,F.H., Li,J., Cai,H.B., Strom,S., Bisello,A., Kelley,D.E., Friedman-Einat,M., Skibinski,G.A., McCrory,M.A., Szalai,A.J., Zhao,A.Z. Induction of leptin resistance through direct interaction of C-reactive protein with leptin. Nature Medicine 2006; 12(-4-):425-432. >>> Leptin, recomb. human; C-reactive protein, human; Saline; SC; Mice; mice (transgenic); 6, 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum concentrations; dose-response (fig.5); peptides; MRI; multiple pumps per animal (2); animal info (wt or ob/ob, 8 wk old, human CRPtg.).
P7888 da Silva,A.A., Tallam,L.S., Liu,J.K., Hall,J.E. Chronic antidiabetic and cardiovascular actions of leptin: role of CNS and increased adrenergic activity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 291(-5-):R1275-R1282. >>> Leptin; Saline; CSF/CNS; Rat; 2002; 10 days; Controls received IV saline; functionality of mp verified by plasma leptin concentrations; comparison of IV infusion vs. mp; cardiovascular; peptides; animal info (male, Sprague-Dawley, 275-325g.).
P7811 Krol,E., Duncan,J.S., Redman,P., Morgan,P.J., Mercer,J.G., Speakman,J.R. Photoperiod regulates leptin sensitivity in field voles, Microtus agrestis. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 2006; 176(-2-):153-163. >>> Leptin, recomb. mouse; PBS; SC; Vole; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides; animal info (male, field vole (microtus agrestis), 20-23 wk old, 26g.); "the osmotic pumps were effective in delivering biologically active hormone to the animals." (pg. 160).
P7717 Suzuki,R., Tobe,K., Aoyama,M., Sakamoto,K., Ohsugi,M., Kamei,N., Nemoto,S., Inoue,A., Ito,Y., Uchida,S., Hara,K., Yamauchi,T., Kubota,N., Terauchi,Y., Kadowaki,T. Expression of DGAT2 in white adipose tissue is regulated by central leptin action. Journal of Biological Chemistry 2005; 280(-5-):3331-3337. >>> Leptin; Saline; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, C57BL/6, 16-18 wk old).
P7710 Lohmus,M., Sundstroem,L.F., Silverin,B. Chronic administration of leptin in Asian Blue Quail. JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-COMPARATIVE EXPERIMENTAL BIOLOGY 2006; 305A(-1-):13-22. >>> Leptin, recomb. chicken; PBS; SC; Bird (quail); 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume at 3 time points; peptides; animal info (male, asian blue quail, 50 g, young).
P7709 Benomar,Y., Wetzler,S., Larue-Achagiotis,C., Djiane,J., Tome,D., Taouis,M. In vivo leptin infusion impairs insulin and leptin signalling in liver and hypothalamus. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2005; 242(-1-2-):59-66. >>> Leptin, ovine; Water; buffer, sodium dihydrogen phosphate; IP; Rat; 2001; 7 days; Controls received mp w/ saline; peptides; animal info (male, wistar 150-160 g, 7-8 wks old).
P7699 Chiellini,C., Costa,M., Novelli,S.E., Amri,E.Z., Benzi,L., Bertacca,A., Cohen,P., Del,Prato S., Friedman,J.M., Maffei,M. Identification of cathepsin K as a novel marker of adiposity in white adipose tissue. J.Cell Physiol 2003/5; 195(-2-):309-321. >>> Leptin, recomb. mouse; SC; Mice; 2002; 3 days; Controls received mp w/ PBS; peptides; animal info (female, C57B/6J, 10 weeks old).
P7685 Alon,T., Friedman,J.M. Late-onset leanness in mice with targeted ablation of melanin concentrating hormone neurons. Journal of Neuroscience 2006; 26(-2-):389-397. >>> Leptin; PBS; Mice; 2002; 9 days; Peptides; animal info (C57BL/6).
P7651 Shore,S.A., Schwartzman,I.N., Mellema,M.S., Flynt,L., Imrich,A., Johnston,R.A., Shore,S.A. Effect of leptin on allergic airway responses in mice. Journal of Allergy and Clinical Immunology 2005; 115(--):103-109. >>> Leptin; Saline; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; post op. care (buprenorphine analgesic); animal info (BALB/cj).
P7648 Hamrick,M.W., la-Fera,M.A., Choi,Y.H., Pennington,C., Baile,C.A. Leptin treatment induces loss of bone marrow adipocytes and increases bone formation in leptin-deficient ob/ob mice. Journal of Bone and Mineral Research 2005; 20(-6-):994-1001. >>> Leptin; Saline; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; peptides; animal info (ob/ob, 15 weeks old, female).
P7642 Sahu,A., Metlakunta,A.S. Hypothalamic phosphatidylinositol 3-kinase-phosphodiesterase 3B-cyclic AMP pathway of leptin signalling is impaired following chronic central leptin infusion. Journal of Neuroendocrinology 2005; 17(-11-):720-726. >>> Leptin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 2002; 9, 23 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; pumps replaced after 7 days; peptides; animal info (male, Sprague-Dawley 250-300 g, adult); all animals received aCSF for 7 days, then agent or vehicle for 2 or 16 days.
P7626 Rousseau,K., Atcha,Z., Denton,J., Cagampang,F.R.A., Ennos,A.R., Freemont,A.J., Loudon,A.S.I. Skeletal bone morphology is resistant to the high amplitude seasonal leptin cycle in the Siberian hamster. Journal of Endocrinology 2005; 186(-3-):475-479. >>> Leptin, recomb. murine; PBS; SC; Hamster; 1007D; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; pumps replaced at 1 week; peptides; animal info (male, Siberian, 16 wk old, 38 g).
P7616 Eiden,S., Simon,E., Schmidt,I. Dose-related steady states of fat loss in long-term leptin-treated ob/ob mice: Leptin resistance or desensitization versus counterregulatory signaling. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 2005; 175(-7-):487-497. >>> Leptin, recomb. murine His 6-tagged; Saline; SC; Mice; 1002; 55, 60 days; Controls received mp w/ agent and were 2 months old; functionality of mp verified by residual volume; dose-response (fig 1); long-term study; pumps replaced every 15 days; no stress (see pg.488); peptides; animal info (C57B1/6J, male, ob/ob; 2 or 5 months old 33 g, 58 g).
P7600 Barouch,L.A., Gao,D.Q., Chen,L., Miller,K.L., Xu,W.H., Phan,A.C., Kittleson,M.M., Minhas,K.M., Berkowitz,D.E., Wei,C.M., Hare,J.M. Cardiac myocyte apoptosis is associated with increased DNA damage and decreased survival in murine models of obesity. Circulation Research 2006; 98(-1-):119-124. >>> Leptin, recomb. mouse; SC; Mice; 1002; 4 weeks; Pumps replaced after 14 days; cardiovascular; peptides; animal info (6-8 month old); obesity.
P7548 Gunduz,Z., Dursun,N., Akgun,H., Ozturk,F., Okur,H., Koc,N. Renal effects of long-term leptin infusion and preventive role of losartan treatment in rats. REGULATORY PEPTIDES 2005; 132(-1-3-):59-66. >>> Leptin, recomb. murine; PBS; IP; Rat; 28 days; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, 218-277 g).
P7526 Toyoshima,Y., Gavrilova,O., Yakar,S., Jou,W., Pack,S., Asghar,Z., Wheeler,M.B., LeRoith,D. Leptin improves insulin resistance and hyperglycemia in a mouse model of type 2 diabetes. Endocrinology 2005; 146(-9-):4024-4035. >>> Leptin; SC; Mice; 2002; 14 days; Controls received mp w/ saline; functionality of mp verified by serum leptin levels; replacement therapy (type 2 diabetes); peptides; animal info (MKR male, 6-7 wk. old, wild type 20-25 g).
P7525 Trevaskis,J.L., Butler,A.A. Double leptin and melanocortin-4 receptor gene mutations have an additive effect on fat mass and are associated with reduced effects of leptin on weight loss and food intake. Endocrinology 2005; 146(-10-):4257-4265. >>> Leptin; Saline; SC; Mice; 2002; 14 days; Comparison of IP&SC injections vs. mp; peptides; animal info (6 month old, female, WT C57BL/6J); mp primed 4 hours in PBS.
P7518 Wilsey,J., Matheny,M.K., Scarpace,P.J. Oral vanadium enhances the catabolic effects of central leptin in young adult rats. Endocrinology 2006; 147(-1-):493-501. >>> Leptin; CSF, artificial; CSF/CNS; Rat; 2004; 21, 28 days; Controls received mp w/ vehicle; dose-response (fig. 7); ALZET brain infusion kit used; peptides; animal info (F344, brown Norway, male, 3 months old, 357-370 g); mp primed 36 hours in 37 C sterile saline.
P7507 Wieland,C.W., Florquin,S., Chan,E.D., Leemans,J.C., Weijer,S., Verbon,A., Fantuzzi,G., van der Poll,T. Pulmonary Mycobacterium tuberculosis infection in leptin-deficient ob/ob mice. International Immunology 2005; 17(-11-):1399-1408. >>> Leptin; SC; Mice; 2004; 18 days; Controls received mp w/ saline; immunology; peptides; animal info (female, C57BL/6, ob/ob or WT, 6-8 wk old).
P7473 Minhas,K.M., Khan,S.A., Raju,S.V.Y., Phan,A.C., Gonzalez,D.R., Skaf,M.W., Lee,K., Tejani,A.D., Saliaris,A.P., Barouch,L.A., O'Donnell,C.P., Emala,C.W., Berkowitz,D.E., Hare,J.M. Leptin repletion restores depressed beta-adrenergic contractility in ob/ob mice independently of cardiac hypertrophy. JOURNAL OF PHYSIOLOGY-LONDON 2005; 565(-2-):463-474. >>> Leptin, recomb. mouse; SC; Mice; 1002; 28 days; Functionality of mp verified by plasma agent levels; long-term study; pumps replaced after 14 days; cardiovascular; peptides; animal info (C57BL/6J wt, ob/ob, 6 wk old).
P7451 Fekete,C., Singru,P.S., Sanchez,E., Sarkar,S., Christoffolete,M.A., Riberio,R.S., Rand,W.M., Emerson,C.H., Bianco,A.C., Lechan,R.M. Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. Endocrinology 2006; 147(-1-):520-529. >>> Leptin, recomb. mouse; insulin; glucose; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; functionality of mp verified by agent levels in CSF+ serum; peptides; post op. care (bacitracin ointment); animal info (male Sprague-Dawley, 200-250 g); cannula placement confirmed by light microscope.
P7430 Hewson,A.K., Tung,L.Y., Connell,D.W., Tookman,L., Dickson,S.L. The rat arcuate nucleus integrates peripheral signals provided by leptin, insulin, and a ghrelin mimetic. Diabetes 2002/12; 51(-12-):3412-3419. >>> Insulin, recomb. human; leptin, recomb. murine; Saline; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; replacement therapy (fa/fa obese); ALZET brain infusion kit 2 used; peptides; mp primed overnight in 37 degree C saline.
P7406 Hillebrand,J.J.G., Koeners,M.P., de Rijke,C.E., Kas,M.J.H., Adan,R.A.H. Leptin treatment in activity-based anorexia. Biological Psychiatry 2005; 58(-2-):165-171. >>> Leptin; Saline, sterile; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; post op. care (buprenorphin); telemetry implants in IP cavity.
P7383 Demas,G.E., Sakaria,S. Leptin regulates energetic tradeoffs between body fat and humoural immunity. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 2005; 272(-1574-):1845-1850. >>> Leptin, recomb. murine; Tris buffer; SC; Hamster; 2002; 10 days; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; obesity; animal info (female, >60 days old, siberian).
P7370 Wilson,M.E., Fisher,J., Brown,J. Chronic subcutaneous leptin infusion diminishes the responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis in female rhesus monkeys. PHYSIOLOGY&BEHAVIOR 2005; 84(-3-):449-458. >>> Leptin; SC; Monkey (rhesus); 28 days; Peptides.
P7347 Sennello,J.A., Fayad,R., Morris,A.M., Eckel,R.H., Asilmaz,E., Montez,J., Friedman,J.M., Dinarello,C.A., Fantuzzi,G. Regulation of T cell-mediated hepatic inflammation by adiponectin and leptin. Endocrinology 2005; 146(-5-):2157-2164. >>> Leptin; adiponectin; PBS; SC; Mice (transgenic); 1007D; 6, 8 days; Functionality of mp verified by plasma levels; peptides.
P7321 Aparicio,T., Kotelevets,L., Tsocas,A., Laigneau,J.P., Sobhani,I., Chastre,E., Lehy,T. Leptin stimulates the proliferation of human colon cancer cells in vitro but does not promote the growth of colon cancer xenografts in nude mice or intestinal tumorigenesis in ApcMin/+ mice. Gut 2005; 54(-8-):1136-1145. >>> Leptin, recomb. human; leptin, recomb. murine; Saline, BSA; SC; Mice (nude); mice; 2002; 4, 6 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; pumps replaced every 2 weeks; cancer (colon, intestinal adenoma); peptides.
P7319 Coppola,A., Meli,R., Diano,S. Inverse shift in circulating corticosterone and leptin levels elevates hypothalamic deiodinase type 2 in fasted rats. Endocrinology 2005; 146(-6-):2827-2833. >>> Leptin, recomb. rat; PBS; SC; Rat; 1003D; 3 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); peptides.
P7314 Martin,A., de Vittoris,R.L., David,V., Moraes,R., Begeot,M., Lafage-Proust,M.H., Alexandre,C., Vico,L., Thomas,T. Leptin modulates both resorption and formation while preventing disuse-induced bone loss in tail-suspended female rats. Endocrinology 2005; 146(-8-):3652-3659. >>> Leptin, recomb. human; IP; Rat; 3, 7, 14 days; Controls received mp w/ vehicle; peptides.
P7175 Cohen,P., Yang,G.Q., Yu,X.X., Soukas,A.A., Wolfish,C.S., Friedman,J.M., Li,C. Induction of leptin receptor expression in the liver by leptin and food deprivation. Journal of Biological Chemistry 2005; 280(-11-):10034-10039. >>> Leptin, recomb.; PBS; SC; Mice; 2002; 6,8,10 days; Controls received mp w/ vehicle; peptides.
P7172 Barazzoni,R., Zanetti,M., Bosutti,A., Biolo,G., Vitali-Serdoz,L., Stebel,M., Guarnieri,G. Moderate caloric restriction, but not physiological hyperleptinemia per se, enhances mitochondrial oxidative capacity in rat liver and skeletal muscle - Tissue-specific impact on tissue triglyceride content and AKT activation. Endocrinology 2005; 146(-4-):2098-2106. >>> Leptin, recomb. murine; Sodium citrate; SC; Rat; 2ML1; 7 days; Controls received mp w/ vehicle; peptides.
P7160 Cettour-Rose,P., Theander-Carrillo,C., Asensio,C., Klein,M., Visser,T.J., Burger,A.G., Meier,C.A., Rohner-Jeanrenaud,F. Hypothyroidism in rats decreases peripheral glucose utilisation, a defect partially corrected by central leptin infusion. Diabetologia 2005; 48(-4-):624-633. >>> Thyroxine; leptin, human analog; triiodothyronine, reverse; Saline, isotonic; SC; Rat; 2001; 3, 6 days; Controls received mp w/ vehicle, functionality of mp verified by plasma levels, replacement therapy (hypothyroidism), enzyme inhibitor (deiodinase), peptides, multiple pumps per animal (2), agents are also known as T3 and T4.
P7151 Rooks,C.R., Penn,D.M., Kelso,E., Bowers,R.R., Bartness,T.J., Harris,R.B.S. Sympathetic denervation does not prevent a reduction in fat pad size of rats or mice treated with peripherally administered leptin. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2005; 289(-1-):R92-R102. >>> Leptin, recomb. murine; PBS; IP; Rat; mice; 1002; 2002; 13 days; Controls received mp w/ vehicle; peptides; obesity.
P7102 Nogueiras,R., Barreiro,M.L., Caminos,J.E., Gaytan,F., Suominen,J.S., Navarro,V.M., Casanueva,F.F., Aguilar,E., Toppar,J., Dieguez,C., Tena-Sempere,M. Novel expression of resistin in rat testis: functional role and regulation by nutritional status and hormonal factors. Journal of Cell Science 2004; 117(-15-):3247-3257. >>> Leptin, recomb. human; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; peptides.
P7100 Lohmus,M., Olin,M., Sundstroem,L.F., Troedsson,M.H.T., Molitor,T.W., El Halawani,M. Leptin increases T-cell immune response in birds. General and Comparative Endocrinology 2004; 139(-3-):245-250. >>> Leptin, recomb. chicken; PBS; SC; Bird (quail); 1002; 2 weeks; Controls received mp w/ vehicle; peptides; asian blue quail.
P7094 Pizarro,M., Balasubramaniyan,N., Solis,N., Solar,A., Duarte,I., Miquel,J.F., Suchy,F.J., Trauner,M., Accatino,L., Ananthanarayanan,M., Arrese,M. Bile secretory function in the obese Zucker rat: Evidence of cholestasis and altered canalicular transport function. Gut 2004; 53(-12-):1837-1843. >>> Leptin; Sodium citrate; SC; Rat; 2ML1; ; Controls received mp w/ vehicle.
P7093 Akirav,E.M., Chan,O., Inouye,K., Riddell,M.C., Matthews,S.G., Vranic,M. Partial leptin restoration increases hypothalamic-pituitary-adrenal activity while diminishing weight loss and hyperphagia in streptozotocin diabetic rats. METABOLISM-CLINICAL AND EXPERIMENTAL 2004; 53(-12-):1558-1564. >>> Leptin, recomb. mouse; PBS; SC; Rat; 4 days; Controls received mp w/ vehicle; replacement therapy (STZ-diabetes); peptides; STZ induced diabetes.
P7071 Hao,J., de Vaca,S.C., Carr,K.D. Effects of chronic ICV leptin infusion on motor-activating effects of D-amphetamine in food-restricted and ad libitum fed rats. PHYSIOLOGY&BEHAVIOR 2004; 83(-3-):377-381. >>> Leptin, recomb. mouse; Saline, sterile; BSA; CSF/CNS; Rat; 2002; 12,14 days; Controls received mp w/ vehicle; peptides.
P7055 Li,G., Zhang,Y., Wilsey,J.T., Scarpace,P.J. Unabated anorexic and enhanced thermogenic responses to melanotan II in diet-induced obese rats despite reduced melanocortin 3 and 4 receptor expression. Journal of Endocrinology 2004; 182(-1-):123-132. >>> Leptin, recomb. mouse; melanotan II; CSF, artificial; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used.
P7045 Bertile,F., Raclot,T. Differences in mRNA expression of adipocyte-derived factors in response to fasting, refeeding and leptin. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 2004; 1683(-1-3-):101-109. >>> Leptin, recomb. rat; PBS; SC; Rat; 2ML2; 4,5-7 days; Controls received mp w/ vehicle; peptides.
P7017 Choi,Y.H., la-Fera,M.A., Li,C.L., Hartzell,D.L., Little,D.E., Kuhar,M.J., Balle,C.A. CART peptide: central mediator of leptin-induced adipose tissue apoptosis?. REGULATORY PEPTIDES 2004; 121(-1-3-):155-162. >>> CART (55-102); leptin, recomb. rat; CSF, artificial; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ vehicle; dose-response; peptides; mp primed overnight; catheter connecting mp to cannula filled with 12 ul aCSF to give 24 hours lag time before agent delivery.
P7007 Crowley,W.R., Ramoz,G., Torto,R., Kalra,S.P. Role of leptin in orexigenic neuropeptide expression during lactation in rats. Journal of Neuroendocrinology 2004; 16(-7-):637-644. >>> Leptin, recomb. rat; insulin, bovine; Saline; SC; Rat; 2001; 3 days; Controls received mp w/ vehicle; peptides.
P7002 Zanetti,M., Barazzoni,R., Vadori,M., Stebel,M., Biolo,G., Guarnieri,G. Lack of direct effect of moderate hyperleptinemia to improve endothelial function in lean rat aorta: role of calorie restriction. Atherosclerosis 2004; 175(-2-):253-259. >>> Leptin, murine; Sodium, citrate; SC; Rat; 2ML1; 1 week; 7 days; Controls received mp w/ vehicle; cardiovascular; peptides.
P6984 Shimamura,M., Matsuda,M., Ando,Y., Koishi,R., Yasumo,H., Furukawa,H., Shimomura,L. Leptin and insulin down-regulate angiopoietin-like protein 3, a plasma triglyceride-increasing factor. Biochemical and Biophysical Research Communications 2004; 322(-3-):1080-1085. >>> Leptin, recomb. mouse; PBS; SC; Mice; 12 days; Controls received mp w/ vehicle.
P6960 McAlister,E.D., Van Vugt,D.A. Effect of leptin administration versus re-feeding on hypothalamic neuropeptide gene expression in fasted male rats. Canadian Journal of Physiology and Pharmacology 2004; 82(-12-):1128-1134. >>> Leptin, recomb. murine; CSF, artificial; SC; CSF/CNS; Rat; 2001; ; Peptides; ICV cannulation performed one week prior to pump implantation.
P6948 Kim,M.S., Namkoong,C., Kim,H.S., Jang,P.G., Pak,Y.M.K., Katakami,H., Park,J.Y., Lee,K.U. Chronic central administration of ghrelin reverses the effects of leptin. International Journal of Obesity 2004; 28(-10-):1264-1271. >>> Leptin; ghrelin; Saline; CSF/CNS (third ventricle); Rat; 7 days; Controls received mp w/ vehicle; plasma levels; peptides; Plastics One cannula used; 7 day recovery from cannulation prior to pump implant; cannulation verified by dipsogenic response to ICV administration of angiotensin II (150 ng/rat), positive response was defined as the consumption of 5 ml of water within 1 hr of injection.
P6899 Montez,J.M., Soukas,A., Asilmaz,E., Fayzikhodjaeva,G., Fantuzzi,G., Friedman,J.M. Acute leptin deficiency, leptin resistance, and the physiologic response to leptin withdrawal. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2005; 102(-7-):2537-2542. >>> Leptin; PBS; SC; Mice; 8 days; Controls received mp w/ vehicle; peptides; 0.5 ul/hr pump used.
P6827 Miyazaki,M., Jacobson,M.J., Man,W.C., Cohen,P., Asilmaz,E., Friedman,J.M., Ntambi,J.M. Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. Journal of Biological Chemistry 2003; 278(-36-):33904-33911. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1002; 12 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); cardiovascular; peptides.
P6826 Huang,L, Wang,Z, Li,C. Modulation of Circulating Leptin Levels by Its Soluble Receptor. Journal of Biological Chemistry 2001; 276(-9-):6343-6349. >>> Leptin, recomb. mouse; SC; Mice; 1002; 12 days; Functionality of mp verified by plasma leptin levels; replacement therapy (ob/ob mice); peptides.
P6810 Delporte,M.L., El Mkadem,S.A., Quisquater,M., Brichard,S.M. Leptin treatment markedly increased plasma adiponectin but barely decreased plasma resistin of ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2004; 287(-3-):E446-E453. >>> Leptin, recomb. mouse; Sodium citrate; SC; Mice; 2001; 3, 6 days; Controls received mp w/ vehicle; replacement therapy (obese (ob/ob) mice); peptides; obesity; diabetes.
P6784 Schirmer,S.H., Buschmann,I.R., Jost,M.M., Hoefer,I.E., Grundmann,S., Andert,J.P., Ulusans,S., Bode,C., Piek,J.J., van Royen,N. Differential effects of MCP-1 and leptin on collateral flow and arteriogenesis. Cardiovascular Research 2004; 64(-2-):356-364. >>> Monocyte chemoattractant protein-1, recomb. human; leptin, recomb. human; IA (femoral); Rabbit; 2ML1; 7 days; Controls received mp w/ PBS; no stress (see pg. 359); cardiovascular; peptides; right femoral artery occlusion.
P6762 Suganami,T., Mukoyama,M., Mori,K., Yokoi,H., Koshikawa,M., Sawai,K., Hidaka,S., Ebihara,K., Tanaka,T., Sugawara,A., Kawachi,H., Vinson,C., Ogawa,Y., Nakao,K. Prevention and reversal of renal injury by leptin in a new mouse model of diabetic nephropathy. FASEB Journal 2004; 18(-13-):U261-U283. >>> Leptin, recomb. mouse; Saline; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentrations; peptides; diabetes.
P6748 Howard,J.K., Cave,B.J., Oksanen,L.J., Tzameli,I., Bjorbaek,C., Flier,J.S. Enhanced leptin sensitivity and attenuation of diet-induced obesity in mice with haploinsufficiency of Socs3. Nature Medicine 2004; 10(-7-):734-738. >>> Leptin, murine; PBS; SC; Mice; 1002; 8 days; Controls received mp w/ PBS; peptides; obesity.
P6678 Figlewicz,D.P., Bennett,J., Evans,S.B., Kaiyala,K., Sipols,A.J., Benoit,S.C. Intraventricular insulin and leptin reverse place preference conditioned with high-fat diet in rats. Behavioral Neuroscience 2004; 118(-3-):479-487. >>> Leptin; insulin; CSF; CSF/CNS (third ventricle); Rat; 7,14 days; Controls received mp w/ vehicle; peptides; behavioral study; one week and two week pump used; models not listed.
P6626 Freeman,D.A., Lewis,D.A., Kauffman,A.S., Blum,R.M., Dark,J. Reduced leptin concentrations are permissive for display of torpor in Siberian hamsters. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2004; 287(-1-):R97-R103. >>> Leptin; PBS; SC; Hamster; 2002; 14 days; Controls received mp w/ vehicle; peptides; telemetry devices used.
P6583 Suzuki,R., Tobe,K., Aoyama,M., Inoue,A., Sakamoto,K., Yamauchi,T., Kamon,J., Kubota,N., Terauchi,Y., Yoshimatsu,H., Matsuhisa,M., Nagasaka,S., Ogata,H., Tokuyama,K., Nagai,R., Kadowaki,T. Both insulin signaling defects in the liver and obesity contribute to insulin resistance and cause diabetes in Irs2-/- mice. Journal of Biological Chemistry 2004; 279(-24-):25039-25049. >>> Leptin; CSF/CNS; Mice; 1003D; ; Controls received mp w/ saline; peptides.
P6547 Aparicio,T., Guilmeau,S., Goiot,H., Tsocas,A., Laigneau,J.P., Bado,A., Sobhani,I., Lehy,T. Leptin reduces the development of the initial precancerous lesions induced by azoxymethane in the rat colonic mucosa. Gastroenterology 2004; 126(-2-):499-510. >>> Leptin, recomb. mouse; Saline; BSA; SC; IP; Intraluminal (colon); Rat; 2ML1; 7,23 days; Controls received mp w/ vehicle; animal info (male, Fisher 344, 280-300 g.); functionality of mp verified by plasma concentrations; comparison of 2 daily colonic lumen injections vs. mp; pumps replaced every 8 days to ensure peptide activity; cancer (colon); peptides.
P6546 Asensio,C., Cettour-Rose,P., Theander-Carrillo,C., Rohner-Jeanrenaud,F., Muzzin,P. Changes in glycemia by leptin administration or high-fat feeding in rodent models of obesity/type 2 diabetes suggest a link between resistin expression and control of glucose homeostasis. Endocrinology 2004; 145(-5-):2206-2213. >>> Leptin, human; Saline, isotonic; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides.
P6489 Asilmaz,E., Cohen,P., Miyazaki,M., Dobrzyn,P., Ueki,K., Fayzikhodjaeva,G., Soukas,A.A., Kahn,C.R., Ntambi,J.M., Socci,N.D., Friedman,J.M. Site and mechanism of leptin action in a rodent form of congenital lipodystrophy. Journal of Clinical Investigation 2004; 113(-3-):414-424. >>> Leptin, recomb. mouse; SC; CSF/CNS (third ventricle); Mice; 12 days; Controls received mp w/ PBS; dose-response (fig.6); peptides.
P6471 Perez,C., Fernandez-Galaz,C., Fernandez-Agullo,T., Arribas,C., Andres,A., Ros,M., Carrascosa,J.M. Leptin impairs insulin signaling in rat adipocytes. Diabetes 2004; 53(-2-):347-353. >>> Leptin, rat; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle.
P6453 Cui,Y.X., Huang,L., Elefteriou,F., Yang,G.Q., Shelton,J.M., Giles,J.E., Oz,O.K., Pourbahrami,T., Lu,C.Y.H., Richardson,J.A., Karsenty,G., Li,C. Essential role of STAT3 in body weight and glucose homeostasis. MOLECULAR AND CELLULAR BIOLOGY 2004; 24(-1-):258-269. >>> Leptin, recomb. human; Saline; CSF/CNS (third ventricle); Mice (knockout, transgenic); 2004; 28 days; ALZET brain infusion kit 2; peptides; obesity; diabetes.
P6284 Elefteriou,F., Takeda,S., Ebihara,K., Magre,J., Patano,N., Kim,C.A., Ogawa,Y., Liu,X., Ware,S.M., Craigen,W.J., Robert,J.J., Vinson,C., Nakao,K., Capeau,J., Karsenty,G. Serum leptin level is a regulator of bone mass. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-9-):3258-3263. >>> Leptin; CSF/CNS; Mice (transgenic); ; ALZET brain infusion kit 2 used; peptides; recomb. human leptin used; no pump listed.
P6253 Lalonde,J., Samson,P., Poulin,S., Deshaies,Y., Richard,D. Additive effects of leptin and topiramate in reducing fat deposition in lean and obese ob/ob mice. PHYSIOLOGY&BEHAVIOR 2004; 80(-4-):415-420. >>> Leptin; PBS; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; obesity.
P6086 Pal,R., Sahu,A. Leptin signaling in the hypothalamus during chronic central leptin infusion. Endocrinology 2003; 144(-9-):3789-3798. >>> Leptin; CSF; artificial; CSF/CNS; Rat; 2002; 16 days; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; plastics one cannula used; first pump filled with aCSF served as a recovery.
P6085 Moraes,R.C., Blondet,A., Birkenkamp-Demtroeder,K., Tirard,J., Orntoft,T.F., Gertler,A., Durand,P., Naville,D., Begeot,M. Study of the alteration of gene expression in adipose tissue of diet-induced obese mice by microarray and reverse transcription-polymerase chain reaction analyses. Endocrinology 2003; 144(-11-):4773-4782. >>> Leptin; PBS; IP; Mice; 1007D; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; obesity.
P6073 Miyanaga,F., Ogawa,Y., Ebihara,K., Hidaka,S., Tanaka,T., Hayashi,S., Masuzaki,H., Nakao,K. Leptin as an adjunct of insulin therapy in insulin-deficient diabetes. Diabetologia 2003; 46(-10-):1329-1337. >>> Leptin; insulin, human; Saline; SC; Mice (transgenic); 1007D; ; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; paper incorrectly states pump model as a 1002D.
P6066 Scarpace,P.J., Matheny,M., Zolotukhin,S., Tumer,N., Zhang,Y. Leptin-induced leptin resistant rats exhibit enhanced responses to the melanocortin agonist MT II. Neuropharmacology 2003; 45(-2-):211-219. >>> Leptin; melanotan II; CSF, artificial; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; obesity; melanotan II (MT II) is a melanocortin receptor agonist; leptin was infused for 7 days, then the pump was replaced with MTII for another 7 days; pump model was not listed.
P6039 Alon,T., Friedman,J.M., Socci,N.D. Cytokine-induced patterns of gene expression in skeletal muscle tissue. Journal of Biological Chemistry 2003; 278(-34-):32324-32334. >>> Leptin; Saline; SC; Mice; 2002; 2 weeks; Controls received mp w/ PBS; peptides.
P6027 Bertile,F., Oudart,H., Le Maho,Y., Raclot,T. Recombinant leptin in the hypothalamic response to late fasting. Biochemical and Biophysical Research Communications 2003; 310(-3-):949-955. >>> Leptin; PBS; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; leptin plasma levels taken; peptides; obesity; multiple doses used.
P6026 Oben,J.A., Roskams,T., Yang,S.Q., Lin,H.Z., Sinelli,N., Li,Z.P., Torbenson,M., Thomas,S.A., Diehl,A.M. Norepinephrine induces hepatic fibrogenesis in leptin deficient ob/ob mice. Biochemical and Biophysical Research Communications 2003; 308(-2-):284-292. >>> Leptin; PBS; SC; Mice; 2 weeks; Peptides; pump model not listed.
P6021 Fan,X.Q., Bradbury,M.W., Berk,P.D. Leptin and insulin modulate nutrient partitioning and weight loss in ob/ob mice through regulation of long-chain fatty acid uptake by adipocytes. Journal of Nutrition 2003; 133(-9-):2707-2715. >>> Leptin; PBS; SC; Mice; 1,2,7,14,21 days; Controls received mp w/ saline; peptides; obesity; catheter attached; pump model not stated.
P6011 Singh,M.K., Krisan,A.D., Crain,A.M., Collins,D.E., Yaspelkis,BB III METABOLISM-CLINICAL AND EXPERIMENTAL 2003; 52(-9-):1196-1205. >>> Leptin; PBS; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; comparison of SC injections vs. mp; peptides.
P5982 Polotsky,V.Y., Li,J.G., Punjabi,N.M., Rubin,A.E., Smith,P.L., Schwartz,A.R., O'Donnell,C.P. Intermittent hypoxia increases insulin resistance in genetically obese mice. JOURNAL OF PHYSIOLOGY-LONDON 2003; 552(-1-):253-264. >>> Leptin; SC; Mice (transgenic); 1007D; 7 days; Peptides.
P5932 Abelenda,M., Ledesma,A., Rial,E., Puerta,M. Leptin administration to cold-acclimated rats reduces both food intake and brown adipose tissue thermogenesis. JOURNAL OF THERMAL BIOLOGY 2003; 28(-6-7-):525-530. >>> Leptin; Saline; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; peptides.
P5920 Barouch,L.A., Berkowitz,D.E., Harrison,R.W., O'Donnell,C.P., Hare,J.M. Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice. Circulation 2003; 108(-6-):754-759. >>> Leptin; PBS; SC; Mice (knockout); 1002; ; Controls received mp w/ vehicle; leptin plasma levels taken; pumps replaced every two weeks; obesity; leptin was recomb. mouse.
P5869 Takeda,S., Elefteriou,F., Levasseur,R., Liu,X.Y., Zhao,L.P., Parker,K.L., Armstrong,D., Ducy,P., Karsenty,G. Leptin regulates bone formation via the sympathetic nervous system. Cell 2002; 111(-3-):305-317. >>> Leptin; MTII; CSF/CNS (third ventricle); Mice; 2004; 28 days; Controls received mp w/ PBS; ALZET brain infusion kit 2 used; peptides; leptin was human recomb.
P5862 Velkoska,E., Morris,M.J., Burns,P., Weisinger,R.S. Leptin reduces food intake but does not alter weight regain following food deprivation in the rat. International Journal of Obesity 2003; 27(-1-):48-54. >>> Leptin, murine; Saline; heparin; IV (femoral); Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by leptin plasma levels; saline pumps replaced after 14 days w/ leptin pumps; peptides; rats received IV saline at 5 ul/hr for the first two weeks; heparin dose 50 U/ml.
P5830 Dobbins,R.L., Szczepaniak,L.S., Zhang,W.G., McGarry,J.D. Chemical sympathectomy alters regulation of body weight during prolonged ICV leptin infusion. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2003; 284(-4-):E778-E787. >>> Leptin; PBS; BSA; CSF/CNS (third ventricle); Rat; 2002; 10 days; Controls received mp w/ vehicle; peptides; leptin dissolved in PBS&0.2% bovine srum albumin; plastics one cannula used.
P5822 Yamauchi,T., Kamon,J., Waki,H., Terauchi,Y., Kubota,N., Hara,K., Mori,Y., Ide,T., Murakami,K., Tsuboyama-Kasaoka,N., Ezaki,O., Akanuma,Y., Gavrilova,O, Vinson,C, Reitman,M.L, Kagechika,H., Shudo,K., Yoda,M., Nakano,Y., Tobe,K., Nagai,R, Kimura,S, Tomita,M., Froguel,P., Kadowaki,T. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 2001; 7(-8-):941-946. >>> Adiponectin; Leptin, mouse recomb; PBS; SC; Mice; 1002; 12 days; Comparison of IP injections vs. SC mp; peptides; obesity research; adiponectin is an adipocyte-derived hormone (adipocytokine).
P5775 Steinberg,G.R., Rush,J.W.E., Dyck,D.J. AMPK expression and phosphorylation are increased in rodent muscle after chronic leptin treatment. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2003; 284(-3-):E648-E654. >>> Leptin, murine; PBS; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; peptides.
P5766 Hidaka,S., Yoshimatsu,H., Kondou,S., Oka,K., Tsuruta,Y., Sakino,H., Itateyama,I., Noguchi,H., Himeno,K., Okamoto,K., Teshima,Y., Okeda,T., Sakata,T. Hypoleptinemia, but not hypoinsulinemia, induces hyperphagia in streptozotocin-induced diabetic rats. J Neurochem 2001; 77(-4-):993-1000. >>> Insulin; Leptin; CSF/CNS (third ventricle); Rat; 2001; 6 days; Controls received mp w/ saline; peptides; leptin was recomb. murine; guide cannula w/ stylet used.
P5741 Bowen,H., Mitchell,T.D., Harris,R.B.S. Method of leptin dosing, strain, and group housing influence leptin sensitivity in high-fat-fed weanling mice. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2003; 284(-1-):R87-R100. >>> Leptin; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; functionality of mp verified by leptin plasma levels, tables p. R92-R93, R97; peptides.
P5705 Takahashi,N., Patel,H.R., Qi,Y., Dushay,J., Ahima,R.S. Divergent effects of leptin in mice susceptible or resistant to obesity. Hormone and Metabolic Research 2002; 34(-11-12-):691-697. >>> Leptin, murine; PBS; SC; Mice; 2001; 2002; 2 weeks; 7 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; peptides.
P5628 Eiden,S., Doering,H., Schmidt,I. Developmental and food-access-dependent changes in effector systems activated by leptin. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2002; 445(-3-):366-374. >>> Leptin; PBS; SC; Rat (neonate); 10 days; Controls received mp w/ vehicle; comparison of injections v. mp; peptides; 14 day and 25 day old rat pups used..
P5613 Chen,H.C., Jensen,D.R., Myers,H.M., Eckel,R.H., Farese,RV Jr Journal of Clinical Investigation 2003; 111(-11-):1715-1722. >>> Leptin; SC; mice; 2001; 5 days; Controls received mp with saline; replacement therapy (genetically leptin deficient mice); pumps replaced after 7 days; peptides.
P5577 Fernandez-Galaz,C., Fernandez-Agullo,T., Perez,C., Peralta,S., Arribas,C., Andres,A., Carrascosa,J.M., Ros,M. Long-term food restriction prevents ageing-associated central leptin resistance in wistar rats. Diabetologia 2002; 45(-7-):997-1003. >>> Leptin; PBS; CSF/CNS; rat; 2001; 6 days; controls received mp with leptin; dose-response (graph, p. 999); peptides.
P5553 Cettour-Rose,P., Burger,A.G., Meier,C.A., Visser,T.J., Rohner-Jeanrenaud,F. Central stimulatory effect of leptin on T-3 production is mediated by brown adipose tissue type II deiodinase. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2002; 283(-5-):E980-E987. >>> Leptin; thyroxine; triiodothyronine; Saline; SC; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; peptides.
P5539 Chen,H.C., Ladha,Z., Farese,RV Jr Endocrinology 2002; 143(-8-):2893-2898. >>> Leptin; SC; CSF/CNS; Mice; 1002; 3, 6 days; Dose-response (graph, p. 2897); peptides; ALZET brain infusion kit 2 used.
P5516 Alavi,K., Schwartz,M.Z., Prasad,R., O'connor,D., Funanage,V. Leptin: a new growth factor for the small intestine. J.Pediatr.Surg. 2002; 37(-3-):327-330. >>> Leptin; BSA; IV; Rat; 2002; 14 days; Dose-response; peptides.
P5514 Scarpace,P.J., Matheny,M., Zhang,Y., Shek,E.W., Prima,V., Zolotukhin,S., Tumer,N. Leptin-induced leptin resistance reveals separate roles for the anorexic and thermogenic responses in weight maintenance. Endocrinology 2002; 143(-8-):3026-3035. >>> Leptin, recomb. mouse; CSF, artificial; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; functionality of mp verified by leptin plasma and CSF levels; peptides; ALZET brain infusion kit used.
P5466 Kondoh,M., Tsukahara,R., Kuronaga,M., Higashimoto,M., Takiguchi,M., Sato,M. Enhancement of MT synthesis by leptin in fasted mice. LIFE SCIENCES 2002; 71(-20-):2425-2433. >>> Leptin; PBS; SC; Mice; 1007D; 6 days; Peptides.
P5459 Kveiborg,M., Chiusaroli,R., Sims,N.A., Wu,M., Sabatakos,G., Horne,W.C., Baron,R. The increased bone mass in Delta FosB transgenic mice is independent of circulating leptin levels. Endocrinology 2002; 143(-11-):4304-4309. >>> Leptin; SC; Mice; 1002; 4 weeks; Controls received mp w/ saline; pumps replaced after 2 weeks; peptides.
P5425 Yaksh,T.L., Scott,B., LeBel,C.L. Effects of continuous lumbar intrathecal infusion of leptin in rats on weight regulation. Neuroscience 2002; 110(-4-):703-710. >>> Leptin; PBS; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; dose-response (p. 703); peptides; recomb. murine leptin used.
P5408 Zhang,Y., Matheny,M., Zolotukhin,S., Tumer,N., Scarpace,P.J. Regulation of adiponectin and leptin gene expression in white and brown adipose tissues: influence of beta 3-adrenergic agonists, retinoic acid, leptin and fasting. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 2002; 1584(-2-3-):115-122. >>> CL-316243; leptin; Saline; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; comparison of single IP injections vs mp; beta 3-adrenergic agonist.
P5407 Zhang,Y., Wilsey,J.T., Frase,C.D., Matheny,M.M., Bender,B.S., Zolotukhin,S., Scarpace,P.J. Peripheral but not central leptin prevents the immunosuppression associated with hypoleptinemia in rats. Journal of Endocrinology 2002; 174(-3-):455-461. >>> Leptin; PBS; SC; Rat; 2001; 1 week; Functionality of mp verified by leptin plasma levels; peptides; murine recomb. leptin used.
P5397 Rousseau,K., Atcha,Z., Cagampang,F.R.A., Le Rouzic,P., Stirland,J.A., Ivanov,T.R., Ebling,F.J.P., Klingenspor,M., Loudon,A.S.I. Photoperiodic regulation of leptin resistance in the seasonally breeding Siberian hamster (Phodopus sungorus). Endocrinology 2002; 143(-8-):3083-3095. >>> Leptin; PBS; SC; Hamster; 1007D; 14 days; Controls received mp w/ vehicle; functionality of mp verified by leptin plasma levels; pumps replaced on day 7; peptides; recomb. murine leptin used.
P5391 Sahu,A. Resistance to the satiety action of leptin following chronic central leptin infusion is associated with the development of leptin resistance in neuropeptide Y neurones. Journal of Neuroendocrinology 2002; 14(-10-):796-804. >>> Leptin; PBS; CSF/CNS; Rat; 2002; 28 days; Controls received mp w/ vehicle; pumps replaced 14 days; stability verified by plasma leptin levels; peptides; 7-day aCSF infusion followed by 28-day leptin infusion followed by another period of aCSF infusion.
P5385 Li,Z., Lin,H., Yang,S., Diehl,A.M. Murine leptin deficiency alters Kupffer cell production of cytokines that regulate the innate immune system. Gastroenterology 2002/10; 123(-4-):1304-1310. >>> Leptin; SC; Mice; 2 weeks; Controls received mp w/ saline; peptides; immunology.
P5349 Madiehe,A.M., Hebert,S., Mitchell,T.D., Harris,R.B.S. Strain-dependent stimulation of growth in leptin-treated obese db/db mice. Endocrinology 2002; 143(-10-):3875-3883. >>> Leptin; PBS; IP; Mice; 1007D; 2004; 7, 24, 26 days; Controls received mp w/ PBS; peptides.
P5280 Hidaka,S., Yoshimatsu,H., Kondou,S., Tsuruta,Y., Oka,K., Noguchi,H., Okamoto,K., Sakino,H., Teshima,Y., Okeda,T., Sakata,T. Chronic central leptin infusion restores hyperglycemia independent of food intake and insulin level in streptozotocin-induced diabetic rats. FASEB J 2002/4; 16(-6-):509-518. >>> Leptin; Saline; SC; CSF/CNS (third ventricle); Rat; 2001; 6 days; Controls received mp w/ vehicle; guide cannula used; 7-day recovery period; leptin serum levels checked; comparison of icv vs. sc leptin administration; peptides.
P5204 Ma,X.H., Muzumdar,R., Yang,X.M., Gabriely,I., Berger,R., Barzilai,N. Aging is associated with resistance to effects of leptin on fat distribution and insulin action. J Gerontol.A Biol Sci.Med.Sci. 2002/6; 57(-6-):B225-B231. >>> Leptin; Saline; SC; Rat; 7 days; Controls received mp/ vehicle: peptides; recomb. mouse leptin.
P5203 Harris,R.B., Mitchell,T.D., Mynatt,R.L. Leptin responsiveness in mice that ectopically express agouti protein. Physiol Behav. 2002/2/1; 75(-1-2-):159-167. >>> Leptin; PBS; IP; Mice; 1002; 13 days; Controls received mp w/ vehicle; functionality verified by leptin serum levels via radioimmunoassay; comparison of icv injections vs. mp; peptides.
P5173 Matson,C.A., Reid,D.F., Ritter,R.C. Daily CCK injection enhances reduction of body weight by chronic intracerebroventricular leptin infusion. Am.J Physiol Regul.Integr.Comp Physiol 2002/5; 282(--):R1368-R1373. >>> Leptin; Saline; CSF/CNS; Rat; 2002; 10 days; Controls received mp w/ vehicle; peptides; "Chronic infusion of leptin by osmotic minipump generally requires much lower leptin doses to produce body weight reductions equivalent to repeated bolus injections" (p.R1368); 1-week recovery period using a guide cannula.
P5166 Steinberg,G.R., Bonen,A., Dyck,D.J. Fatty acid oxidation and triacylglycerol hydrolysis are enhanced after chronic leptin treatment in rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2002; 282(--):E593-E600. >>> Leptin; PBS; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by leptin serum levels; peptides;.
P5160 Jacobson,L. Middle-aged C57BL/6 mice have impaired responses to leptin that are not improved by calorie restriction. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2002; 282(--):E786-E793. >>> Leptin, mouse; PBS; SC; mice; 3, 4, or 10 days; controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; peptides; dose-ranging study.
P5115 Steinberg,G.R., Dyck,D.J., Calles-Escandon,J., Tandon,N.N., Luiken,J.J.F.P., Glatz,J.F.C., Bonen,A. Chronic leptin administration decreases fatty acid uptake and fatty acid transporters in rat skeletal muscle. Journal of Biological Chemistry 2002; 277(-11-):8854-8860. >>> Leptin; PBS; SC; rat; 2ML2; 2 weeks; controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides.
P5098 Watanobe,H, Suda,T, Wikberg,J.E.S., Schioth,H.B. Evidence that physiological levels of circulating leptin exert a stimulatory effect on luteinizing hormone and prolactin surges in rats. Biochemical and Biophysical Research Communications 1999; 263(--):162-165. >>> Leptin; PBS; SC; rat; 2001; 3 days; controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; peptides.
P5064 Bernal-Mizrachi,C., Weng,S., Li,B., Nolte,L.A., Feng,C., Coleman,T., Holloszy,J.O., Semenkovich,C.F. Respiratory uncoupling lowers blood pressure through a leptin-dependent mechanism in genetically obese mice. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2002; 22(--):961-968. >>> Leptin; PBS; IP; mice (transgenic); 2001; 7 days; controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; cardiovascular; leptin was recombinant mouse leptin..
P5056 Chen,H.C., Smith,S.J., Ladha,Z., Jensen,D.R., Ferreira,L.D., Pulawa,L.K., McGuire,J.G., Pitas,R.E., Eckel,R.H., Farese,RV Jr Journal of Clinical Investigation 2002; 109(-8-):1049-1055. >>> Leptin, recombinant mouse; PBS; SC; rat (neonate); 1002; 2002; 5 days; controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; dose-response (page 135); peptides; 1002 pumps used in 15-day old pups, 2002 used in 24-day old pups; His6-tagged murine leptin used.
P4969 Overton,J.M., Williams,T.D., Chambers,J.B., Rashotte,M.E. Central leptin infusion attenuates the cardiovascular and metabolic effects of fasting in rats. Hypertension 2001; 37(--):663-669. >>> Leptin, mouse; PBS; CSF/CNS; rat; 1007D; 6 days; controls received mp w/ vehicle; obesity; Plastics One guide cannula used; guide cannula placement confirmed by the presence of a vigorous drinking response to ICV injection of Angiotensin II;.
P4953 Pearson,P.Y., O'Connor,D.M., Schwartz,M.Z. Novel effect of leptin on small intestine adaptation. Journal of Surgical Research 2001; 97(--):192-195. >>> Leptin; Bovine serum albumin; IV (jugular); rat; 2002; 14 days; controls received mp w/ vehicle; peptides; rats had undergone 80% small intestine resection; Short Bowel Syndrome;.
P4932 Boozer,C.N., Leibel,R.L., Love,R.J., Cha,M.C., Aronne,L.J. Synergy of sibutramine and low-dose leptin in treatment of diet-induced obesity in rats. METABOLISM-CLINICAL AND EXPERIMENTAL 2001; 50(-8-):889-893. >>> Sibutramine; Leptin; Saline; Sodium citrate; SC; rat; 2002; 2ML4; 7 days; controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; dose response (table 3 p. 892); rats given either sibutramine, leptin, both, or vehicle; sibutramine is an antianorexigenic agent; leptin dissolved in sodium citrate vehicle, sibutramine in saline vehicle; Sibutramine + Leptin group received multiple pumps per animal (2)..
P4923 Drazen,D.L., Demas,G.E., Nelson,R.J. Leptin effects on immune function and energy balance are photoperiod dependent in Siberian hamsters (Phodopus sungorus). Endocrinology 2001; 142(--):2768-2775. >>> Leptin; Tris buffer; SC; hamster; 2002; 2 weeks; controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; dose-response (graph p. 2770); "Leptin infusion significantly increased serum leptin levels in both long- and short-day hamsters after both 8 and 13 days of treatment (P<0.05 in all cases)." p. 2770; 3-day surgical recovery period.
P4837 Burguera,B., Hofbauer,L.C., Thomas,T., Gori,F., Evans,G.L., Khosla,S., Riggs,B.L., Turner,R.T. Leptin reduces ovariectomy-induced bone loss in rats. Endocrinology 2001; 142(-8-):3546-3553. >>> Leptin, recombinant human; SC; Rat; Model not mentioned; ; Controls received mp w/ vehicle; functionality of mp verified by plasma and CSF leptin levels; replacement therapy (orariectomy - see comments); dose-repsponse (p. 3549 table 1); peptides; estrogen replacement given via SC injections, not ALZET pump.
P4732 Correia,M.L.G, Morgan,D.A, Sivitz,W.I, Mark,A.L, Haynes,W.G Hypertension 2001; 37(--):936-942. >>> Leptin, recomb. mouse;; IV (jugular); CSF/CNS (third ventricle);; rat;; 2002; 2004; 2ML4;; 2, 4 weeks;; Controls received mp w/ vehicle; functionality of mp verified by CSF and plasma leptin levels; peptides; antihypertensive; Methylene blue stain and microtone slicing to enfure cannula placement; High and low dose studies using 2002, 2004, respectively;.
P4711 Atcha,Z, Cagampang,F.R.A, Stirland,J.A, Morris,I.D., Brooks,A.N, Ebling,F.J.P, Klingenspor,M, Loudon,A.S.I. Leptin acts on metabolism in a photoperiod-dependent manner, but has no effect on reproductive function in the Seasonally Breeding Siberian Hamster (Phodopus sungorus).. Endocrinology 2000; 141(-11-):4128-4135. >>> Leptin, recomb. mouse;; PBS;; SC;; hamster;; 1007D;; 14 days;; Controls received mp w/ vehicle; functionality of mp verified by leptin plasma levels; long-term study, pumps replaced after seven days; peptides;.
P4621 Exner,C, Hebebrand,J, Remschmidt,H, Wewetzer,C, Ziegler,A, Herpertz,S, Schweiger,U, Blum,W.F, Preibisch,G, Heldmaier,G, Klingenspor,M Molecular Psychiatry 2000; 5(--):476-481. >>> Leptin, recomb. mouse; Saline;; SC;; rat;; 2001;; 7 days;; controls received mp w/vehicle; functionality of mp verified by serum leptin levels at end of study.
P4608 Scarpace,P.J, Matheny,M Am.J.Physiol. 1998; 275(--):E259-E264. >>> Leptin;; Saline;; SC;; rat;; 1003D;; 3 days;; controls received mp w/vehicle; comparison of IP injections vs. mp; peptides; 3 pumps implanted concomitantly in each animal..
P4605 Gavrilova,O, Leon,L.R, Marcus-Samuels,B, Mason,M.M, Castle,A.L, Refetoff,S, Vinson,C, Reitman,M.L PNAS 1999/12/7; 96(-25-):14623-14628. >>> Leptin;; Saline, sterile;; SC;; mice;; 2001;; 7 days;; controls received mp w/vehicle;.
P4604 Yaspelkis,B.B, Ansari,L, Ramey,E.L, Holland,G.J, Loy,S.F Metabolism 1999; 48(-5-):671-676. >>> Leptin;; PBS;; SC;; rat;; 2ML2;; 14 days;; controls received mp w/vehicle; peptides; recomb. mouse leptin used..
P4600 Chinookoswong,N., Wang,J.-L., Shi,Z.-Q. Leptin restores euglycemia and normalizes glucose turnover in insulin-deficient diabetes in the rat.. Diabetes 1999/7; 48(--):1487-1492. >>> Leptin, recomb. mouse;; PBS;; SC;; rat;; 12-14 days;; controls received mp w/vehicle; peptides; recomb. methionine murine leptin used;.
P4567 Rafael,J, Herling,A.W Am.J.Physiol.Regulatory Integrative Comp.Physiol. 2000; 278(--):R790-R795. >>> Leptin; PBS; SC; Mice; 1002; 15-75 days; Controls received mp w/vehicle; functionality of mp verified by plasma levels; long-term study, pumps replaced every 2 weeks; peptides.
P4544 Sindelar,D.K, Havel,P.J., Seeley,R.J., Wilkinson,C.W., Woods,S.C., Schwartz,M.W. Low plasma leptin levels contribute to diabetic hyperphagia in rats.. Diabetes 1999/6; 48(--):1275-1280. >>> Leptin;; Saline;; SC;; rat;; 7 days;; controls received mp w/vehicle; functionality of mp verified by plasma levels; peptides; recomb. rat leptin used;.
P4537 Pelleymounter,M.A., Cullen,M.J., Healy,D., Hecht,R., Winters,D., McCaleb,M Am.J.Physiol.(Regulatory Integrative Comp.Physiol.44) 1998; 275(--):R950-R959. >>> Leptin;; PBS;; SC;; mice;; 1007D;; 7 days;; controls received mp w/vehicle; half-life (p. R951); peptides; r-Met-murine leptin used;.
P4513 Pelleymounter,M.A., Baker,M.B., McCaleb,M Am.J.Physiol.(Endocrinol.Metab.39) 1999; 276(--):E955-E963. >>> Leptin;; PBS;; SC;; mice;; 2002; 1007D;; 14 days;; controls received mp w/vehicle; peptides; incorrectly mentions pump model as 2001D for 14 days (should be 2002).
P4489 Arvaniti,K, Deshaies,Y., Richard,D. Effect of leptin on energy balance does not require the presence of intact adrenals.. Am.J.Physiol.(Regulatory Integrative Comp.Physiol.44) 1998; 44(--):R105-R111. >>> Leptin, recomb. mouse;; Saline, sterile;; mice;; 2001;; ; controls received mp w/vehicle; peptides;.
P4454 Ioffe,E, Moon,B, Connolly,E, Friedman,J.M. Abnormal regulation of the leptin gene in the pathogenesis of obesity. Proc.Natl.Acad.Sci. 1998/9; 95(--):11852-11857. >>> Leptin;; PBS;; SC;; mice (transgenic);; 28 days;; functionality of mp verified by plasma levels; pumps replaced after 14 days; peptides; recomb. murine leptin used;.
P4368 Schneider,J.E., Zhou,D. Interactive effects of central leptin and peripheral fuel oxidation on estrous cyclicity. Am.J.Physiol.(Regulatory Integrative Comp.Physiol.46) 1999; 277(--):R1020-R1024. >>> Leptin;; CSF, artificial;; CSF/CNS;; hamster;; 1003D;; 3 days;; controls received mp with vehicle; peptides; murine leptin used;.
P4358 Shi,Z.Q., Nelson,A., Whitcomb,L., Wang,J., Cohen,A.M. Intracerebroventricular administration of leptin markedly enhances insulin sensitivity and systemic glucose utilization in conscious rats. Metabolism 1998/10; 47(-10-):1274-1280. >>> Leptin;; Dulbecco's phosphate buffer;; CSF/CNS;; rat;; 2002;; 7 days;; controls received mp with vehicle; comparison of bolus injections vs. mp; peptides; rat-metMuleptin used.
P4346 Nishiyama,M., Makino,S., Asaba,K., Hashimoto,K. Leptin effects on the expression of type-2 CRH receptor mRNA in the ventromedial hypothalamus in the rat. Journal of Neuroendocrinology 1999; 11(--):307-314. >>> Leptin;; HCl;; SC;; rat;; 2ML1; 5 days;; controls received mp with vehicle; comparison of single bolus I.P. injections vs. mp; peptides; recomb. human leptin used;.
P4269 Halaas,J.L., Boozer,C., Blair-West,J., Fidahusein,N., Denton,D.A., Friedman,J.M. Physiological response to long-term peripheral and central leptin infusion in lean and obese mice. Proc.Natl.Acad.Sci.USA 1997/8; 94(--):8878-8883. >>> Leptin;; PBS; CSF, artificial;; SC; CSF/CNS (third ventricle);; mice; 30 days; controls received mp w/ vehicle; functionality of mp verified by plasma levels in SC infusion studies; peptides; recomb. murine leptin used; animals were infused with aCSF for one week before leptin or PBS was infused for 30 days, followed by 14 days of aCSF infusion; animals that did not respond to ICV leptin received ICV angiotensin II to confirm cannula placement..
P4147 Wang,J.-L., Chinookoswong,N., Scully,S., Qi,M., Shi,Q.-Z. Differential effects of leptin in regulation of tissue glucose utilization in vivo. Endocrinology 1999; 140(-5-):2117-2124. >>> Leptin, recomb. mouse; Phosphate buffer; SC; rat; 2ML1; 5, 7 days; peptides; functionality of mp verified by leptin plasma levels; controls received mp w/vehicle.
P3961 Barzilai,N., Wang,J., Massilon,D., Vuguin,P., Hawkins,M., Rossetti,L. Leptin selectively decreases visceral adiposity and enhances insulin action. J.Clin.Invest. 1997; 100(-12-):3105-3110. >>> Leptin, recomb. mouse; Saline; SC; rat; 2ML1; 8 days; controls received mp w/vehicle; peptides.
P3915 Al-Barazanji,K.A., Buckingham,R.B., Arch,J.R.S., Haynes,A., Mossakowska,D.E., McBay,D.L., Holmes,S.D., McHale,M.T., Wang,X.-T., Gloger,I.S. Effects of intracerebroventricular infusion of leptin in obese Zucker rats. Obesity Research 1997; 5(-5-):387-394. >>> Leptin, recomb. mouse; PBS; CSF/CNS; rat; 2001; 14 days; controls received mp w/PBS; animals received 1 week PBS infusion, then pumps replaced w/mp w/either agent or vehicle; peptides; ALZET brain infusion kit used.
P3888 Dawson,R., Pelleymounter,M.A., Millard,W.J., Liu,S., Eppler,B. Attenuation of leptin-mediated effects by monosodium glutamate-induced arcuate nucleus damage. Am.J.Physiol.(Endocrinol.Metab.) 1997; 273(--):E202-E206. >>> Leptin, recomb. mouse; PBS; rat (pregnant); 2ML2; 14 days; controls received mp w/vehicle; peptides; "Implantation with osmotic minipumps containing leptin resulted in significant elevation in serum leptin levels." (pg. E203).
P3827 Wade,G.N., Lempicki,R.L., Panicker,A.K., Frisbee,R.M., Blaustein,J.D. Leptin facilitates and inhibits sexual behavior in female hamsters. Am.J.Physiol. 1997; 272(-4 Pt2-):R1354-R1358. >>> Leptin; SC; hamster; 1003D; 3 days; controls received mp w/vehicle; comparison of sc injections vs mp; peptides; "The fact that leptin is so much more effective when infused continuously than when injected once every 12 h is probably due to the rapid clearance of injected leptin." (pg. R1356).
P3798 Halaas,J.L., Friedman,J.M. Leptin and its receptor. J Endocrinol 1997; 155(-2-):215-216. >>> Leptin; PBS; CSF/CNS (third ventricle); Mice; 26 days; Controls received w/ vehicle; pumps replaced after 14 days with ACSF containing pumps; peptides; 4-day surgical recovery period achieved by infusion of ACSF; 4-day ACSF infusion followed by 14 day Leptin infusion followed by 10 day ACSF infusion.
P3793 Boyer,B.B., Ormseth,O.A., Buck,L., Nicolson,M., Pelleymounter,M.A., Barnes,B.M. Leptin prevents posthibernation weight gain but does not reduce energy expenditure in arctic ground squirrels. Comp.Biochem.Physiol. 1997; 118C(-3-):405-412. >>> Leptin, recomb. mouse; SC; squirrel; 2ML1; 3 weeks; controls received mp w/saline; functionality of mp verified by plasma leptin levels; pumps replaced weekly; no stress (see pg. 408); peptides.
P3772 Scarpace,P.J, Nicolson,M., Matheny,M J Endocrinol 1998; 159(-2-):349-357. >>> Leptin, mouse; Saline; SC; Rat; 2ML1; 7 days; Controls received mp w/ saline; peptides; serum leptin concentrations taken.
P3712 Huang,Q., Rivest,R., Richard,D. Effects of leptin on corticotropin-releasing factor (CRF) synthesis and CRF neuron activation in the paraventricular hypothalamic nucleus of obese (ob/ob) mice. Endocrinology 1998; 139(-4-):1524-1532. >>> Leptin, recomb. mouse; SC; mice; 1007D; 7 days; controls received mp w/ PBS; peptides.
P3711 Harris,R.B.S., Zhou,J., Redmann Jr,S.M., Smagin,G.N., Smith,S.R., Rodgers,E., Zachwieja,J.J. A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. Endocrinology 1998; 139(-1-):8-19. >>> Leptin, recomb. human; PBS; IP; mice; 1007D; 7 days; dose-response; peptides; "one or two daily injections ... causes excessive, intermittent elevations of the serum leptin concentration." (pg. 8).
P2691 Pelleymounter,M.A., Cullen,M.J., Baker,M.B., Hecht,R., Winters,D., Boone,T., Collins,F. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 1995; 269(--):540-543. >>> Leptin; PBS; SC; mice; 1007D; 21 days; controls received mp w/ PBS; comparison of ip injections vs mp; pumps replaced every 7 days; peptides.
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Researchers are saying...
“We administered nicotine to achieve a steady-state concentration, using subcutaneously implanted minipumps, a technique that avoids the episodic hypoxic-ischemic episodes that accompany nicotine injections, and that also sidesteps the inherent problems of repeated handling stress and episodic withdrawal between injections” Slotkin et al., Neurotoxicology and Teratology 2002; 24:369-384.