Long-Term Infusion Using ALZET^® Osmotic Pumps

R0239 Richardson,R.T., Noushi,F., O'Leary,S. Inner ear therapy for neural preservation. AUDIOLOGY AND NEURO-OTOLOGY 2006; 11(-6-):343-356. >>> Nerve growth factor; NT-3; adenovirus; brain-derived neurotrophic factor; perilymph, artificial; glial-derived neurotrophic factor; ciliary neurotrophic factor; fibroblast growth factor, acidic; fibroblast growth factor-1; fibroblast growth factor-2; fibroblast growth factor, basic; Ear (cochlea); ear (scala tympani); Guinea-pig; 1,2,4,8 weeks; 15-60, 11-12, 26 days; Comparison of polymers, hydrogels, gene therapy, cell-based therapy, and injections vs. mp; long-term study; pumps replaced; no stress (see pg. 350); half-life (p. 344), short in blood; gene therapy; peptides; animal info (deafened); Table 2; "The mini-osmotic pump device is ideally suited to studying the effects of neurotrophic factors in the cochlea experimentally." (p. 350).

P9141 Seigel,G.M., Lupien,S.B., Campbell,L.A., Ishii,D.N. Systemic IGF-I treatment inhibits cell death in diabetic rat retina. Journal of Diabetes and Its Complications 2006; 20(-3-):196-204. >>> Insulin-like growth factor-1; streptozotocin; Acetate; SC; Rat; 2002; 8 weeks; Controls received mp w/ vehicle; long-term study; animal info (Wistar, 12 wks old); diabetes.

P9041 Signolet,I.L., Bousquet,P.P., Monassier,L.J.P. Improvement of cardiac Diastolic function by long-term centrally mediated sympathetic inhibition in one-kidney, one-clip hypertensive rabbits. American Journal of Hypertension 2008; 21(-1-):54-60. >>> Rilmenidine; metoprolol; SC; Rabbit; 2ML2; 6 weeks; Controls received mp w/ vehicle; animal info (New Zealand, White, 4-6 wks old, male, 0.7-0.9 kg., nephrectomized); long-term study.

P9032 Fukuda,D., Sata,M., Ishizaka,N., Nagai,R. Critical role of bone marrow angiotensin II type 1 receptor in the pathogenesis of atherosclerosis in apolipoprotein E-deficient mice. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2008; 28(-1-):90-96. >>> Angiotensin II; Mice; 8 weeks; Controls received mp w/ vehicle; long-term study; peptides; animal info (ApoE -/-, AT1aR -/-).

P9029 Recinos,A.III, LeJeune,W.S., Sun,H., Lee,C.Y., Tieu,B.C., Lu,M., Hou,T., Boldogh,I., Tilton,R.G., Brasier,A.R. Angiotensin II induces IL-6 expression and the Jak-STAT3 pathway in aortic adventitia of LDL receptor-deficient mice. Atherosclerosis 2007; 194(-1-):125-133. >>> Angiotensin II; SC; Mice; 7 weeks; Controls were sham operated; long-term study; pumps replaced after 4 weeks; peptides; animal info (LDLR -/-, 7 wks old).

P9010 Ichihara,A., Kaneshiro,Y., Takemitsu,T., Sakoda,M., Nakagawa,T., Nishiyama,A., Kawachi,H., Shimizu,F., Inagami,T. Contribution of nonproteolytically activated prorenin in glomeruli to hypertensive renal damage. Journal of the American Society of Nephrology 2006; 17(-9-):2495-2503. >>> Prorenin, hand6 region decapeptide, rat; SC; Rat; 2004; 8 weeks; Controls received mp w/ saline; long-term study; pumps replaced after 4 weeks; peptides; animal info (male, Wistar-Kyoto, SHRsp, 4 wks old, 100-150 g.); nephrology.

P9001 Stenstroem,B., Zhao,C.M., Tommeraas,K., Arum,C.J., Chen,D. Is gastrin partially responsible for body weight reduction after gastric bypass?. European Surgical Research 2006; 38(-2-):94-101. >>> Gastrin-17, synthetic rat; Saline; BSA; SC; Rat; 2 months; Controls received no treatment; functionality of mp verified by serum gastrin concentrations; long-term study; pumps replaced every 2 weeks; peptides; animal info (male, Sprague Dawley, 2 months old, 230 g., gastric bypass).

P8996 Galatz,L.M., Silva,M.J., Rothermich,S.Y., Zaegel,M.A., Havlioglu,N., Thomopoulos,S. Nicotine delays tendon-to-bone healing in a rat shoulder model. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME 2006; 88A(-9-):2027-2034. >>> Nicotine; SC; Rat; 2ML4; 4, 8 weeks; 10 days; Controls received mp w/ saline; functionality of mp verified by nicotine and continine blood levels; long-term study; pumps replaced after 4 weeks; animal info (male, Sprague Dawley, 350-400 g.); rotator cuff healing model.

P8984 Flamant,M., Placier,S., Rodenas,A., Curat,C.A., Vogel,W.F., Chatziantoniou,C., Dussaule,J.C. Discoidin domain receptor 1 null mice are protected against hypertension-induced renal disease. Journal of the American Society of Nephrology 2006; 17(-12-):3374-3381. >>> Angiotensin II; SC; Mice (transgenic); 4, 6 weeks; Functionality of mp verified by systolic blood pressure; pumps replaced; peptides; animal info (male, 129/Sv x CD1, wt, DDR1 -/-, 4-6 months old, 30-35 g.); nephrology; long-term study; pump model incorrectly stated as 1004.

P8980 Mueller,M.A., Beutner,F., Teupser,D., Ceglarek,U., Thiery,J. Prevention of atherosclerosis by the mTOR inhibitor everolimus in LDLR(-/-) mice despite severe hypercholesterolemia. Atherosclerosis 2008; 198(-1-):39-48. >>> SDZ RAD; Ethanol; propylene glycol; SC; Mice; 2004; 12 weeks; Long-term study; pumps replaced every 28 days; animal info (LDL receptor deficient, male); SDZ RAD also known as everolimus or certican; compound is a mamallian target of rapamycin inhibitor.

P8979 Susic,D., Zhou,X.Y., Frohlich,E.D., Lippton,H., Knight,M. Cardiovascular effects of prorenin blockade in genetically spontaneously hypertensive rats on normal and high-salt diet. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1117-H1121. >>> PRAM-1; Water, distilled; SC; Rat; 2ML4; 4, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks; stability verified; cardiovascular; peptides; animal info (male, SHR, 20 wks old); PRAM-1 is a custom synthesized prorenin inhibitor; "no degradation products of the peptide were observed".

P8972 Prabhakar,S., Starnes,J., Shi,S., Lonis,B., Tran,R. Diabetic nephropathy is associated with oxidative stress and decreased renal nitric oxide production. Journal of the American Society of Nephrology 2007; 18(-11-):2945-2952. >>> Insulin, porcine; Glycerine, ethanol; SC; Rat; 2004; 12 weeks; Controls received no treatment; long-term study; pumps replaced every 4 wks; multiple pumps per animal (2); animal info (ZSF1, 8 wks old, 200-250 g.); 50% ethanol used.

P8929 Badlani,N., Inoue,A., Healey,R., Coutts,R., Amiel,D. The protective effect of OP-1 on articular cartilage in the development of osteoarthritis. OSTEOARTHRITIS AND CARTILAGE 2008; 16(-5-):600-606. >>> Osteogenic protein-1; Lactose; Knee (articular cavity); Rabbit; 2004; 6 weeks; Controls received mp w/ vehicle; functionality of mp verified by separate in vivo test; peptides; post op. care ("appropriate"); animal info (New Zealand, White, 1 yr old); also known as BMP-7 or bone morphogenic protein-7; catheter was secured with polyethylene stents; "The efficacy of the (ALZET Pump) was tested in vivo before beginning the study with good results"; "constant infusion of OP-1 may be the most ideal form of delivery"; long-term study; good methods.

P8926 Cui,G.L., Takaishi,S., Ai,W.D., Betz,K.S., Florholmen,J., Koh,T.J., Houghton,J., Pritchard,D.M., Wang,T.C. Gastrin-induced apoptosis contributes to carcinogenesis in the stomach. LABORATORY INVESTIGATION 2006; 86(-10-):1037-1051. >>> Gastrin-17; SC; Mice; 8 weeks; Controls received mp w/ PBS; functionality of mp verified by serum gastrin levels; long-term study; pumps replaced; cancer (gastric); peptides; animal info (male, GAS-KO, 2 months old).

P8923 Deseure,K., Breand,S., Colpaert,F.C. Curative-like analgesia in a neuropathic pain model: Parametric analysis of the dose and the duration of treatment with a high-efficacy 5-HT1A receptor agonist. European Journal of Pharmacology 2007; 568(-1-3-):134-141. >>> F13640; Acetate buffer; water, sterile; SC; Rat; 2ML1; 2ML2; 2ML4; 2, 4, 8 weeks; 1, 2, 4, 7 days; Controls received mp w/ vehicle; dose-response (fig. 2); long-term study; pumps replaced; half-life (p. 139) 2.5 hrs. in rat plasma; multiple pumps per animal (2); post op. care (Na ampicillin); animal info (male, Sprague Dawley, 220-240 g., intraorbital nerve ligation).

P8915 Lujan,M.E., Krzemien,A.A., Reid,R.L., Van Vugt,D.A. Effect of leptin administration on ovulation in food-restricted rhesus monkeys. Neuroendocrinology 2006; 84(-2-):103-114. >>> Leptin, recomb. human; Water, sterile; CSF/CNS; Monkey; 2002; 32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 wks; stress/adverse reaction: (see pg. 104) 1 animal removed from study due to CNS infection at 28 weeks; peptides; animal info (female, Rhesus, 6-10 yrs. old, 5-12 kg.); endocrinology.

P8876 Viquez,O.M., Valentine,H.L., Amarnath,K., Milatovic,D., Valentine,W.M. Copper accumulation and lipid oxidation precede inflammation and myelin lesions in N,N-diethyldithiocarbamate peripheral myelinopathy. TOXICOLOGY AND APPLIED PHARMACOLOGY 2008; 229(-1-):77-85. >>> Diethyldithiocarbamate, sodium; IM (abdominal); Rat; 2ML4; 2, 4, 8 weeks; Functionality of mp verified by residual volume, visual inspection for blockage; long-term study; pumps replaced after 4 weeks; animal info (male, Sprague Dawley).

P8813 Kubota,Y., Takubo,K., Suda,T. Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche. Biochemical and Biophysical Research Communications 2008; 366(-2-):335-339. >>> Uridine, bromodeoxy-; PBS; SC; Mice; 2004; 2 months; Long-term study; pumps replaced after 28 days; multiple pumps per animal (2); animal info (C57BL/6, 1 month old); labeling of bone marrow LRC's.

P8812 Minnaard-Huiban,M., Emmen,J.M.A., Roumen,L., Beugels,I.P.E., Cohuet,G.M.S., van Essen,H., Ruijters,E., Pieterse,K., Hilbers,P.A.J., Ottenheijm,H.C.J., Plate,R., de Gooyer,M.E., Smits,J.F.M., Hermans,J.J.R. Fadrozole reverses cardiac fibrosis in spontaneously hypertensive heart failure rats: Discordant enantioselectivity Versus reduction of plasma aldosterone. Endocrinology 2008; 149(-1-):28-31. >>> Fadrozole, R-; fadrozole, S-; Sodium phosphate buffer; PEG 400; SC; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; dose-response (Fig. 1); long-term study; pumps replaced after 4 weeks; no stress (see pg. 29); cardiovascular; animal info (male, lean SHHF, 40 wks old, 450g.); aldosterone biosynthesis inhibitor.

P8787 Sharma,U., Rhaleb,N.E., Pokharel,S., Harding,P., Rasoul,S., Peng,H.M., Carretero,O.A. Novel anti-inflammatory mechanisms of N-Acetyl-Ser-Asp-Lys-Pro in hypertension-induced target organ damage. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-3-):H1226-H1232. >>> Angiotensin II; peptide, Ac-SDKP; Saline; acetic acid; SC; Mice; 2004; 8 weeks; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; long-term study; pumps replaced after 4 weeks; cardiovascular; peptides; animal info (male, C57BL6/J, 12-14 wks old).

P8773 Lu,H., Rateri,D.L., Feldman,D.L., Charnigo,RJ Jr, Fukamizu,A., Ishida,J.J., Oesterling,E.G., Cassis,L.A., Daugherty,A. Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice. Journal of Clinical Investigation 2008; 118(-3-):984-993. >>> Aliskiren; PBS; SC; Mice; 2004; 4, 12 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma Aliskiren levels; dose-response (fig. 1); long-term study; pumps replaced every 28 days; no stress (see pg. 985); enzyme inhibitor (Renin); cardiovascular; animal info (male, C57BL/6, Ldlr -/-, 6-8 wks old, 30g.).

P8739 Qiao,X.T., Ziel,J.W., Mckimpson,W., Madison,B.B., Todisco,A., Merchant,J.L., Samuelson,L.C., Gumucio,D.L. Prospective identification of a multilineage progenitor in murine stomach epithelium. Gastroenterology 2007; 133(-6-):1989-1998. >>> Interferon-gamma; PBS; BSA; IP; Mice (transgenic); 2, 8, 12 weeks; Controls received mp w/ vehicle; comparison of IP injections vs. mp; peptides; animal info (12.4 KVil-EGFP tg; C57BL/6J; 4 months old); long-term study.

P8723 Brandt,C., Glien,M., Gastens,A.M., Fedrowitz,M., Bethmann,K., Volk,H.A., Potschka,H., Loescher,W.F. Prophylactic treatment with levetiracetam after status epilepticus: Lack of effect on epileptogenesis, neuronal damage, and behavioral alterations in rats. Neuropharmacology 2007; 53(-2-):207-221. >>> Levetriacetam; Water, distilled; SC; Rat; 2ML2; 8 weeks; Controls received mp w/ saline; functionality of mp verified by residual volume; long-term study; pumps replaced three times every 14 days; stress/adverse reaction (see pg. 210); half life (p. 209) 2-3 hours; animal info (female, Sprague-Dawley, 200-200g, epileptic); levetiracetam is an antiepileptic drug.

P8717 Brooks,D.E., Komaromy,A.M., Kallberg,M.E., Miyabashi,T., Ollivier,F.J., Lambrou,G.N. Blood flow velocity response of the ophthalmic artery and anterior optic nerve head capillaries to carbogen gas in the rhesus monkey model of optic nerve head ischemia. VETERINARY OPHTHALMOLOGY 2007; 10(-;-):20-27. >>> Endothelin-1; CSF/CNS (optic nerve); Monkey (Rhesus); 2004; 8 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; peptides; animal info (young, male, Rhesus); glaucoma.

P8709 Wen,J., Wang,P.Y., Smith,S.V., Haller,C.A., Chaikof,E.L. Syndecans are differentially expressed during the course of aortic aneurysm formation. JOURNAL OF VASCULAR SURGERY 2007; 46(-5-):1014-1025. >>> Angiotensin II; SC; Mice; 1002; 2004; 1, 4, 8 weeks; funtionality of mp verified by systolic blood pressure; long-term study; pumps replaced after 4 weeks; cardiovascular; peptides; animal info (male, C57BL/6, ApoE -/-, 8 wks old).

P8656 Jesmin,S., Maeda,S., Mowa,C.N., Zaedi,S., Togashi,H., Prodhan,S.H., Yamaguchi,T., Yoshioka,M., Sakuma,I., Miyauchi,T., Kato,N. Antagonism of endothelin action normalizes altered levels of VEGF and its signaling in the brain of stroke-prone spontaneously hypertensive rat. European Journal of Pharmacology 2007; 574(-2-3-):158-171. >>> SB209670; Saline; SC; Rat; 2ML4; 12 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; ischemia (cerebral); animal info (SHRSP, WKY, 6 wks old); endothelin dual receptor antagonist.

P8653 Bichler,E.K., Carrasco,D.I., Rich,M.M., Cope,T.C., Pinter,M.J. Rat motoneuron properties recover following reinnervation in the absence of muscle activity and evoked acetylcholine release. JOURNAL OF PHYSIOLOGY-LONDON 2007; 585(-1-):47-56. >>> Tetrodotoxin; Penicillin G potassium; gentamicin sulfate; CSF/CNS (posterior tibial nerve); Rat; 2004; 4, 8 weeks; Controls received mp w/ saline; long-term study; pumps replaced after 28 days; no stress (see pg. 52); animal info (female, Wistar, 270-380g, MG nerve crush; used silastic nerve cuff.

P8649 Sancho-Bru,P., Bataller,R., Fernandez-Varo,G., Moreno,M., Ramalho,L.N., Colmenero,J., Mari,M., Claria,J., Jimenez,W., Arroyo,V., Brenner,D.A., Gines,P. Bradykinin attenuates hepatocellular damage and fibrosis in rats with chronic liver injury. Gastroenterology 2007; 133(-6-):2019-2028. >>> Bradykinin; Saline; SC; Rat; mice; 1007D; 2004; 10 weeks; 6 days; Controls received mp w/ vehicle; dose-response (fig 2); long-term study; pumps replaced; no stress (see pg. 2026); animal info (male, Wistar, 205g; male, C57/BL6, 8 wks old); nephrology.

P8642 Takahashi,H., Ichihara,A., Kaneshiro,Y., Inomata,K., Sakoda,M., Takemitsu,T., Nishiyama,A., Itoh,H. Regression of nephropathy developed in diabetes by (Pro)renin receptor blockade. Journal of the American Society of Nephrology 2007; 18(-7-):2054-2061. >>> Prorenin, receptor blocker; peptide, M30TRISAE36; SC; Rat; 2004; 12 weeks; Controls received mp w/ vehicle peptide M30TRISAE36; replacement therapy (heminephrectomy, STZ-induced diabetes); long-term study; pumps replaced every 28 days; peptides; animal info (male, Sprague-Dawley, 17 wks old).

P8636 Shao,T.C., Li,H., Ittmann,M., Cunningham,G.R. Effects of dutasteride on prostate growth in the large probasin-large T antigen mouse model of prostate cancer. Journal of Urology 2007; 178(-4-):1521-1527. >>> Dutasteride; DMSO; PEG 400; SC; Mice (transgenic); 2004; 4, 8 weeks; Controls received mp w/ vehicle; comparison of oral admin. vs. mp; long-term study; pumps replaced after 4 weeks; no stress (see pg. 1522); enzyme inhibitor (5a-reductase); cancer (prostate); animal info (male, LPB-Tag, tg, wt, 6-7 and 10-11 wks old); 40% DMSO; "Dutasteride greatly decreased prostate tumor growth... without significant negative effects." p. 1526.

P8617 Carro,E., Trejo,J.L., Busiguina,S., Torres-Aleman,I. Circulating Insulin-Like Growth Factor I Mediates the Protective Effects of Physical Exercise against Brain Insults of Different Etiology and Anatomy. Journal of Neuroscience 2001; 21(-15-):5678-5684. >>> Antibody, anti-IGF-I; serum, rabbit; insulin-like growth factor I, recomb.; Saline; SC; Rat; mice; 1002; 2004; 4-6 weeks; 14 days; Controls received mp w/ normal rabbit serum; long-term study; peptides; animal info (male, C57BL/6, 25 grams, male, wistar, 250-300 grams).

P8572 Surwit,R.S., Edwards,C.L., Murthy,S., Petro,A.E. Transient effects of long-term leptin supplementation in the prevention of diet-induced obesity in mice. Diabetes 2000; 49(-7-):1203-1208. >>> Leptin, recomb. mouse; SC; Mice; 2004; 12 weeks; Controls received mp w/ PBS; functionality of mp verified by plasma levels and residual volume; long-term study; pumps replaced every 4 weeks; no stress (see pg. 1205); peptides; animal info (male, C57BL/6J, B6, A/J, 20 grams); obesity.

P8567 Zhang,J., Tokui,Y., Yamagata,K., Kozawa,J., Sayama,K., Iwahashi,H., Okita,K., Miuchi,M., Konya,H., Hamaguchi,T., Namba,M., Shimomura,I., Miyagawa,J.I. Continuous stimulation of human glucagon-like peptide-1 (7-36) amide in a mouse model (NOD) delays onset of autoimmune type 1 diabetes. Diabetologia 2007; 50(-9-):1900-1909. >>> Glucagon-like peptide-1; Acetic acid buffer, 5%; SC; Mice; 2004; 4 or 8 weeks; Controls received mp w/ saline; functionality of mp verified by GLP-1 plasma levels; long-term study; pumps replaced after 4 weeks; animal info (female, NOD mice, 8 weeks old); diabetes; "plasma concentrations of active GLP-1...after pump implantation were significantly elevated and about 1.5 fold higher than before the pump implantation. We therefore considered this delivery system to be appropriate for this experiment." (p. 1903).

P8559 Samuel,C.S., Lin,F., Hossain,M.A., Zhao,C.X., Ferraro,T., Bathgate,R.A.D., Tregear,G.W., Wade,J.D. Improved chemical synthesis and demonstration of the relaxin receptor binding affinity and biological activity of mouse relaxin. Biochemistry 2007; 46(-18-):5374-5381. >>> Relaxin, synthetic mouse; SC; Mice; 2004; 4 months; Controls received no treatment; dose-response (fig. 4); long-term study; pumps replaced every month; half-life (p. 5376) 10-15 minutes; peptides; animal info (Rln -/-, 2 months old, male).

P8516 Hong,L.Z., Hsieh,P.S. Hyperinsulinemia Instead of Insulin Resistance Induces Baroreflex Dysfunction in Chronic Insulin-Infused Rats. American Journal of Hypertension 2007; 20(-4-):451-458. >>> Insulin, porcine; Glycerin; ethanol; SC; Rat; 2002; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 14 days; post op. care (penicillin); animal info (male, Sprague-Dawley, 200-250 grams); 50% ETOH used [manufacturer suggests 15% or less ETOH; for pump compatibility purposes].

P8513 Gomori,A., Ishihara,A., Ito,M., Matsushita,H., Ito,M., Mashiko,S., Iwaasa,H., Matsuda,M., Bednarek,M.A., Qian,S., MacNeil,D.J., Kanatani,A. Blockade of MCH1 receptor signalling ameliorates obesity and related hepatic steatosis in ovariectomized mice. Br.J.Pharmacol. 2007/7; 151(-6-):900-908. >>> Melanin-concentrating hormone 1, receptor antagonist; Propylene glycol; water, distilled; CSF/CNS; Mice; 2004; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks of vehicle delivery; ALZET brain infusion kit used; post op. care (cefamezin-a); animal info (female, C57BL/6J, 12 weeks old, female Mch1r KD, 14-16 weeks old, 22 g); cannula placement confirmed with Evans Blue dye; obesity.

P8478 Ma,T., Gutnick,J., Salazar,B., Larsen,M.D., Suenaga,E., Zilber,S., Huang,Z., Huddleston,J., Smith,R.L., Goodman,S. Modulation of allograft incorporation by growth factors over a prolonged continuous infusion of duration in vivo. Bone 2007; 41(-3-):386-392. >>> Fibroblast growth factor-2; osteogenic protein-1, recomb. human; Water, distilled; acetate buffer; Bone (tibia); Rabbit; 2004; 4,36 weeks; Controls received no treatment to contralateral side; long-term study; pumps replaced every 4 weeks, with 4 week rest periods between 4 week infusion periods; animal info (New Zealand white, 6-12 months old, 3.5-4.2 kg); peptides; OP-1 also known as bone morphogenic protein-7 (BMP-7); unique drug test chamber and bone harvest chamber used in conjunction with mp.

P8477 Mack,C., Wilson,J., Athanacio,J., Reynolds,J., Laugero,K., Guss,S., Vu,C., Roth,J., Parkes,D. Pharmacological actions of the peptide hormone amylin in the long-term regulation of food intake, food preference, and body weight. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 293(-5-):R1855-R1863. >>> Amylin; DMSO; SC; Rat; 2ML4; 4,8,11 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma amylin concentrations; dose-response (fig 1); comparison of IP injections vs. mp; long-term study; pumps replaced every 4 weeks; half-life (p. R1861) "short;" peptides; animal info (male, Sprague-Dawley, Levin DIOP); 50% DMSO used; endocrinology; "Due to its short half-life, amylin was administered via subcutaneous infusion as a proof of concept for its potential use as an antiobesity agent." (p. R1861).

P8443 Banach-Petrosky,W., Jessen,W.J., Ouyang,X., Gao,H., Rao,J., Quinn,J., Aronow,B.J., bate-Shen,C. Prolonged exposure to reduced levels of androgen accelerates prostate cancer progression in Nkx3.1; Pten mutant mice. Cancer Research 2007; 67(-19-):9089-9096. >>> Testosterone propionate; PEG 400; SC; Mice; 2004; 7 months; Controls received mp w/ vehicle, or no treatment; replacement therapy (castration); dose-response (fig 1A); long-term study; pumps replaced every 28 days; cancer prostate; animal info (male, C57Bl/65X129/SvImJ, 6 wks old).

P8436 Rabbani,Z.N., Batinic-Haberle,I., Anscher,M.S., Huang,J., Day,B.J., Alexander,E., Dewhirst,M.W., Vujaskovic,Z. Long-term administration of a small molecular weight catalytic metalloporphyrin antioxidant, AEOL 10150, protects lungs from radiation-induced injury. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 2007; 67(-2-):573-580. >>> AEOL 10150; SC; Rat; 2002; 10 weeks; 8 days; Controls received mp w/ vehicle; functionality of mp verified by plasma AEOL 10150 concentrations; dose-response (fig. 4); long-term study; pumps replaced every 2 weeks; half-life (p. 574), short; cancer; animal info (female, Fisher-344, 150-170 grams); "Osmotic mini-pumps provided consistent and dose-dependent delivery of AEOL 10150," "a continuous availability of antioxidant via osmotic infusion pumps throughout the study." (p. 575).

P8405 Zhang,X.C., Jia,Y.H., Jackson,E.K., Tofovic,S.P. 2-methoxyestradiol and 2-ethoxyestradiol retard the progression of renal disease in aged, obese, diabetic ZSF1 rats. Journal of Cardiovascular Pharmacology 2007; 49(-1-):56-63. >>> Estradiol, 2-hydroxy-; estradiol, 1-methoxy-; PEG 400; Rat; 2ML4; 9 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 32 days; no stress (see pg. 58-9); cardiovascular; animal info (male, obese ZSF1,35 wks old, 650-375g.); nephrology.

P8397 Park,J.H., Widi,G.A., Gimbel,D.A., Harel,N.Y., Lee,D.H.S., Strittmatter,S.M. Subcutaneous Nogo receptor removes brain amyloid-beta and improves spatial memory in Alzheimer's transgenic mice. Journal of Neuroscience 2006; 26(-51-):13279-13286. >>> Nogo-66 receptor (310) ecto-Fc, rat; immunoglobulin G, rat; PBS; SC; Mice (transgenic); 2004; 12 weeks; Controls received mp w/ rat IgG; long-term study; pumps replaced every 28 days; animal info (APPswe/PSEN-1delta E9, 7-10 months old); neurodegenerative (Alzheimer's disease).

P8343 Bone,W., Walden,C.M., Fritsch,M., Voigtmann,U., Leifke,E., Gottwald,U., Boomkamp,S., Platt,F.M., van der Spoel,A.C. The sensitivity of murine spermiogenesis to miglustat is a quantitative trait: a pharmacogenetic study. Reproductive Biology and Endocrinology 2007; 5(--):U1-U13. >>> Miglustat; SC; Mice; 2004; 7 weeks; Functionality of mp verified by serum agent levels; comparison of oral vs. mp; long-term study; enzyme inhibitor (ceramide glucosyltransferase); animal info (male, C57BL/6J, BALB/C, FVB/N, 6 weeks old); reproductive endocrinology.

P8323 Shibata,M.A., Morimoto,J., Doi,H., Morishima,S., Naka,M., Otsuki,Y. Electrogene therapy using endostatin, with or without suicide gene therapy, suppresses murine mammary tumor growth and metastasis. Cancer Gene Therapy 2007; 14(-3-):268-278. >>> Ganciclovir; Saline; SC; Mice; 2002; 8 weeks; Controls received mp w/ agent and empty control vector; long-term study; pumps replaced every 14 days; no stress (see pg. 271); cancer (mammary adenocarcinoma); animal info (BALB/C, female, 20-24 grams).

P8289 Banach-Petrosky,W., Ouyang,X.S., Gao,H., Nader,K., Ji,Y., Suh,N., DiPaola,R.S., bate-Shen,C. Vitamin D inhibits the formation of prostatic intraepithelial neoplasia in Nkx3.1; Pten mutant mice. Clinical Cancer Research 2006; 12(-19-):5895-5901. >>> Vitamin D3, 1,25-dihydroxy-; Propylene glycol; SC; Mice; 2004; 1-4 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; stability verified by serum calcium levels; cancer (prostate); animal info (4-7 months old, male).

P8282 Birkeland,J.A.K., Sjaastad,I., Brattelid,T., Qvigstad,E., Moberg,E.R., Krobert,K.A., Bjornerheim,R., Skomedal,T., Sejersted,O.M., Osnes,J.B., Levy,F.O. Effects of treatment with a 5-HT4 receptor antagonist in heart failure. British Journal of Pharmacology 2007; 150(-2-):143-152. >>> SB207266; SC; Rat; 2ML4; 6 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 3 weeks; stress/adverse reaction: (see pg. 145), 1 animal managed to remove the mp [ALZET: This could be due to the agent being irritating or a number of additional variables]; cardiovascular; animal info (male, Wistar, 320 g, MI by left coronary artery ligation).

P8243 Saxe,M.D., Malleret,G., Vronskaya,S., Mendez,I., Garcia,A.D., Sofroniew,M.V., Kandel,E.R., Hen,R. Paradoxical Influence of hippocampal neurogenesis on working memory. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-11-):4642-4646. >>> Ganciclovir; Saline, sterile; SC; Mice (transgenic); 2004; 8 weeks; Controls received mp w/ agent but did not contain GFAP-Tk Tg; long-term study; pumps replaced after 4 weeks, with 2 weeks in between implantations; no stress (see pg. 4643); animal info (male, C57BL/6-BALB/C, 12-20 weeks old); "Because this system has previously been shown to cause gastrointestinal toxicity at high doses (16), we delivered a constant low dose of GCV for several weeks via SC osmotic minipumps...This modification achieved a nearly complete reduction in hippocampal neurogenesis...but resulted in no effects on activity, body weight, food consumption, or (GI) pathology." (p. 4643).

P8176 Radeloff,A., Smolders,J.W.T. Brain-derived neurotrophic factor treatment does not improve functional recovery after hair cell regeneration in the pigeon. Acta Oto-laryngologica 2006; 126(-5-):452-459. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, chicken; Hank's solution; Ear (scala tympani); Bird (pigeon); 2002; 8 weeks; Controls received mp w/ vehicle or no treatment to contralateral ear; long-term study; pumps replaced every 14 days; ALZET brain infusion kit used; peptides; animal info (Columba livia, 6 months old, 320-580 grams); deafening of both ears; tissue perfusion (scala tympani).

P8167 Bidani,A.K., Picken,M., Hacioglu,R., Williamson,G., Griffin,K.A. Spontaneously reduced blood pressure load in the rat streptozotocin-induced diabetes model: potential pathogenetic relevance. American Journal of Physiology-Renal Physiology 2007; 292(-2-):F647-F654. >>> Insulin; SC; Rat; 16-20, 36-40 weeks; Replacement therapy (STZ-induced Diabetes); long-term study; pumps replaced every 4 weeks; peptides; animal info (male, Sprague-Dawley, 8 weeks old, 250 grams).

P8161 Balatsouka,D., Gotfredsen,K., Sorensen,L., Lindh,C.H., Berglundh,T. Effect of systemic administration of nicotine on healing in osseous defects. An experimental study in rabbits. Part II. CLINICAL ORAL IMPLANTS RESEARCH 2006; 17(-5-):488-494. >>> Nicotine hydrogen tartrate salt; Water, sterile; SC; Rabbit; 2ML4; 8 weeks; Controls received mp w/ saline; functionality of mp verified by cotinine plasma levels; long-term study; pumps replaced after 4 weeks; multiple pumps per animal (2); animal info (female, New Zealand white, 9-12 months old, 3-4 kg, osseous defects).

P8079 Nakamura,S., Murayama,N., Noshita,T., Katsuragi,R., Ohno,T. Cognitive dysfunction induced by sequential injection of amyloid-beta and ibotenate into the bilateral hippocampus; protection by memantine and MK-801. European Journal of Pharmacology 2006; 548(-1-3-):115-122. >>> Memantine; MK-801; Saline; SC; Rat; 2ML2; 6 weeks; 11 days; Controls received mp w/ vehicle; dose-response (fig.2); long-term study; pumps replaced every 2 weeks; animal info (male, F344/DuCrj, 10 wk old, 220-250g.); neurodegenerative (Alzheimers's disease).

P8042 Wente,W., Efanov,A.M., Brenner,M., Kharitonenkov,A., Koester,A., Sandusky,G.E., Sewing,S., Treinies,I., Zitzer,H., Gromada,J. Fibroblast growth factor-21 improves pancreatic beta-cell function and survival by activation of extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Diabetes 2006; 55(-9-):2470-2478. >>> Fibroblast growth factor-21; Saline; SC; Mice; 1002; 8 weeks; FGF-21 plasma levels taken; long-term study; pumps replaced every two weeks; animal info (C57BL/6, 8 weeks old); diabetes.

P8020 Ma,T., Nelson,E.R., Mawatari,T., Oh,K.J., Larsen,D.M., Smith,R.L., Goodman,S.B. Effects of local infusion of OP-1 on particle-induced and NSAID-induced inhibition of bone ingrowth in vivo. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 2006; 79A(-3-):740-746. >>> OP-1, recomb. human; Acetate buffer; Bone (tibia); Rabbit; 2004; 6, 24 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4-6 weeks; stress/adverse reaction (see pg. 742) implant (DTC and mp) infection in one animal [manufacturer note: pumps are provided sterile]; peptides; post op. care (cephalosporin); animal info (male, New Zealand white, 6-12 months old, 3.5-4.2 kg); agent also know as bone morphogenic protein-7; mp implanted with a titanium drug test chamber (DTC).

P7996 Viquez,O.M., Valentine,H.L., Friedman,D.B., Olson,S.J., Valentine,W.M. Peripheral nerve protein expression and carbonyl content in N,N-diethlydithiocarbamate myelinopathy. Chemical Research In Toxicology 2007; 20(-3-):370-379. >>> Diethlydithiocarbamate, N, N-; IP; Rat; 2ML4; 8 weeks; Long-term study; pumps replaced every 4 weeks; animal info (adult, male, Sprague-Dawley, 351 grams).

P7963 Rubel,E.W., Dew,L.A  Science 1995; 267(-5198-):701-707. >>> Thymidine; 3H tracer; radio-isotopes; Ear (semicircular canal); Guinea-pig; 2002; 2,4,6 weeks; Functionality of mp verified by scintillation counts of perilymph of the ear; long-term study; pumps replaced every two weeks; ALZET brain infusion kit used; animal info (albino, 300-400 g); Vetbond adhesive used to close skin; tissue perfusion (semicircular canal).

P7875 Lund,A.K., Goens,M.B., Nunez,B.A., Walker,M.K. Characterizing the role of endothelin-1 in the progression of cardiac hypertrophy in aryl hydrocarbon receptor (AhR) null mice. TOXICOLOGY AND APPLIED PHARMACOLOGY 2006; 212(-2-):127-135. >>> BQ123; Saline; SC; Mice; 2004; 7, 28, 58 days; 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced on day 29; cardiovascular; peptides; animal info (C57B6N, AhR -/-, wt, 2 month old, 5 month old, 20-30g.); ET-A receptor antagonist.

P7866 Lupien,S.B., Bluhm,E.J., Ishii,D.N. Effect of IGF-I on DNA, RNA, and protein loss associated with brain atrophy and impaired learning in diabetic rats. NEUROBIOLOGY OF DISEASE 2006; 21(-3-):487-495. >>> Insulin-like growth factor-I; Acetic acid; SC; Rat; 7-8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 18 days; peptides; neurodegenerative (Alzheimer's disease); animal info (male, Harlan Wistar, 10 wk. old).

P7853 Jesmin,S., Zaedi,S., Maeda,S., Togashi,H., Yamaguchi,I., Goto,K., Miyauchi,T. Endothelin antagonism suppresses plasma and cardiac endothelin-1 levels in SHRSPs at the typical hypertensive stage. EXPERIMENTAL BIOLOGY AND MEDICINE 2006; 231(-6-):919-924. >>> SB209670; Saline; SC; Rat; 2ML4; 12 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; cardiovascular; animal info (male, SHRSP, 6 wk. old, Wistar-Kyoto); ETA/ETB dual receptor antagonist.

P7850 Ichihara,A., Kaneshiro,Y., Takemitsu,T., Sakoda,M., Suzuki,F., Nakagawa,T., Nishiyama,A., Inagami,T., Hayashi,M. Nonproteolytic activation of prorenin contributes to development of cardiac fibrosis in genetic hypertension. Hypertension 2006; 47(-5-):894-900. >>> Prorenin decapeptide, rat; peptides, synthetic; SC; Rat; 2004; 8 weeks; Controls received mp w/ saline; long-term study; pumps replaced after 4 weeks; cardiovascular; peptides; animal info (male, Wistar Kyoto, SHR, 4 wk. old).

P7809 Ma,J., Weisberg,A., Griffin,J.P., Vaughan,D.E., Fogo,A.B., Brown,N.J. Plasminogen activator inhibitor-1 deficiency protects against aldosterone- induced glomerular injury. Kidney International 2006; 69(-6-):1064-1072. >>> Aldosterone, D-; DMSO; SC; Mice; 2004; 12 weeks; Controls received mp w/ vehicle; replacement therapy (uninephrectomy); long-term study; pumps replaced every 4 weeks; cardiovascular; animal info (male, C57BL/6J wt or PAI-1(-/-), 8-10 wk old, 27g.); 3.6% DMSO; nephrology.

P7777 Fan,Y.Y., Baba,R., Nagai,Y., Miyatake,A., Hosomi,N., Kimura,S., Sun,G.P., Kohno,M., Fujita,M., Abe,Y., Nishiyama,A. Augmentation of intrarenal angiotensin II levels in uninephrectomized aldosterone/salt-treated hypertensive rats; Renoprotective effects of an ultrahigh dose of olmesartan. HYPERTENSION RESEARCH 2006; 29(-3-):169-178. >>> Aldosterone; Ethanol; SC; Rat; 2002; 3, 6 weeks; Controls received mp w/ vehicle; replacement therapy (uninephrectomy); long-term study; pumps replaced every 2 weeks; cardiovascular; animal info (male, Sprague-Dawley, 162-184g.); 2% ETOH.

P7756 Shibuya,K., Kanasaki,K., Isono,M., Sato,H., Omata,M., Sugimoto,T., Araki,S.I., Isshiki,K., Kashiwagi,A., Haneda,M., Koya,D. N-acetyl-seryl-aspartyl-lysyl-proline prevents renal insufficiency and mesangial matrix expansion in diabetic db/db mice. Diabetes 2005; 54(-3-):838-845. >>> Peptide, Ac-SDKP; Balanced salt solution; SC; Mice; 2004; 8 weeks; Controls received mp w/ vehicle, or controld peptide; functionality of mp verified by plasma AC-SDKP levels; replacement therapy (diabetes); long-term study; pumps replaced at 4 weeks; no stress (see pg. 839, 843); peptides; animal info (male, db/db, db/m, 10 wk. old).

P7750 Suzuki,J., Iwai,M., Li,Z., Li,J.M., Min,L.J., Ide,A., Yoshii,T., Oshita,A., Mogi,M., Horiuchi,M. Effect of combination of calcium antagonist, azelnidipine, and AT1 receptor blocker, olmesartan, on atherosclerosis in apolipoprotein E-deficient mice. Journal of Hypertension 2005; 23(-7-):1383-1389. >>> Olmesartan; IP; Mice; 1002; 10 weeks; Dose-response (fig.1); long-term study; pumps replaced every 2 weeks; cardiovascular; animal info (adult, male, ApoE -/-, 6 wk. old, 25-30g.).

P7740 Sekhon,H.S., Song,P.F., Jia,Y.B., Lindstrom,J., Spindel,E.R. Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure. Cell and Tissue Research 2005; 320(-2-):287-297. >>> Nicotine tartrate; Water, bacteriostatic; SC; Monkey (pregnant); 2ML4; 19 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 3 weeks; post op. care (cefazolin); animal info (female, Rhesus, gestation day 26-160); amniocentesis.

P7708 Gohlke,P., Linz,W., Scholkens,B.A., Kuwer,I., Bartenbach,S., Schnell,A., Unger,T. Angiotensin-converting enzyme inhibition improves cardiac function. Role of bradykinin. Hypertension 1994/4; 23(-4-):411-418. >>> Hoe 140; Saline, physiological; SC; Rat; 14 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 weeks; cardiovascular; animal info (SHR, stroke prone, 6 weeks old, male).

P7681 Colpaert,F.C., Deseure,K., Stinus,L., Adriaensen,H. High-efficacy 5-hydroxytryptamine 1A receptor activation counteracts opioid hyperallodynia and affective conditioning. Journal of Pharmacology and Experimental Therapeutics 2006; 316(-2-):892-899. >>> F13640; morphine, HCl; Saline; SC; Rat; 2ML2; 6 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every two weeks; multiple pumps per animal (2); animal info (male, Sprague-Dawley, 220-240 g); during pump replacement a new incision was made about 1 cm away from the previous incision.

P7633 Bao,L.H., Osawe,I., Haas,M., Quigg,R.J. Signaling through up-regulated C3a receptor is key to the development of experimental lupus nephritis. Journal of Immunology 2005; 175(-3-):1947-1955. >>> C3aR; DMSO; Mice; 2001; 6 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every week; half-life (p. 1948), 1.5 hours; animal info (BALB/c, male); 50% DMSO used.

P7621 Narai,H., Nagano,I., Ilieva,H., Shiote,M., Nagata,T., Hayashi,T., Shoji,M., Abe,K. Prevention of spinal motor neuron death by insulin-like growth factor-1 associating with the signal transduction systems in SOD(G93A) transgenic mice. Journal of Neuroscience Research 2005; 82(-4-):452-457. >>> Insulin-like growth factor I, recomb. human; CSF, artificial; CSF/CNS (intrathecal, subarachnoid space, lumbar); Mice (transgenic); 2004; 5-10 weeks; Controls received mp w/ vehicle; dose-response (fig 1); long-term study; pumps replaced every 4 weeks; neurodegenerative (Amyotrophic Lateral Sclerosis); animal info (C57BL/6J, female).

P7606 Kadoya,T., Oyanagi,K., Kawakami,E., Hasegawa,M., Inagaki,Y., Sohma,Y., Horie,H. Oxidized galectin-1 advances the functional recovery after peripheral nerve injury. Neuroscience Letters 2005; 380(-3-):284-288. >>> Galectin-1, recomb. human oxidized; CSF/CNS (sciatic nerve); Rat; 2ML4; 12 weeks; Controls received mp w/ PBS; dose-response (fig. 1); long-term study; no stress (see pg. 285); peptides; animal info (male, adult, Sprague-Dawley); "No rats showed any toxic effects or reaction to the administration of rh GAL-1/Ox." (p. 285).

P7602 Iwai,M., Chen,R., Li,Z., Shiuchi,T., Suzuki,J., Ide,A., Tsuda,M., Okumura,M., Min,L.J., Mogi,M., Horiuchi,M. Deletion of angiotensin II type 2 receptor exaggerated atherosclerosis in apolipoprotein E-null mice. Circulation 2005; 112(-11-):1636-1643. >>> Valsartan; IP; Mice; 1002; 10 weeks; Controls received no treatment; long-term study; cardiovascular; animal info (male); AT1 receptor blocker.

P7575 Zhan,G.X., Serrano,F., Fenik,P., Hsu,R., Kong,L.H., Pratico,D., Klann,E., Veasey,S.C. NADPH oxidase mediates hypersomnolence and brain oxidative injury in a murine model of sleep apnea. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 2005; 172(-7-):921-929. >>> Apocynin; DMSO; SC; Mice; 1002; 8 weeks; Controls received mp w/ vehicle or PBS; long-term study; pumps replaced; enzyme inhibitor (NADPH oxidase); animal info (male, C57BL/6J, 10 wk old).

P7554 Lautrette,A., Li,S.Q., Alili,R., Sunnarborg,S.W., Burtin,M., Lee,D.C., Friedlander,G., Terzi,F. Angiotensin II and EGF receptor cross-talk in chronic kidney diseases: a new therapeutic approach. Nature Medicine 2005; 11(-8-):867-874. >>> Angiotensin II; Saline; SC; Mice; mice (transgenic); 2004; 2 months; 7 days; Controls received mp w/ vehicle; replacement therapy (nephrectomy); long-term study; pumps replaced at 1 month; peptides; animal info (C57Bl6, female, 1 week old).

P7458 Schaefer,S., Schmidts,H.L., Bleich,M., Busch,A.E., Linz,W., Schaefer,S. Nephroprotection in Zucker diabetic fatty rats by vasopeptidase inhibition is partly bradykinin B2 receptor dependent. British Journal of Pharmacology 2004; 143(--):27-32. >>> Icatibant; SC; Rat; 26 weeks; Controls received no treatment; long-term study; pumps replaced every 4 weeks; animal info (ZDF, 21 weeks old, diabetic nephropathy); bradykinin B2 receptor antogonist.

P7401 Donners,M.M.P.C., Bot,I., De Windt,L.J., van Berkel,T.J.C., Daemen,M.J.A.P., Biessen,E.A.L., Heeneman,S. Low-dose FK506 blocks collar-induced atherosclerotic plaque development and stabilizes plaques in ApoE-/- mice. AMERICAN JOURNAL OF TRANSPLANTATION 2005; 5(-6-):1204-1215. >>> FK-506; PBS; SC; Mice; 2004; 4,8 weeks; Controls received mp w/ vehicle; plasma levels taken; long-term study; pumps replaced every 4 weeks.

P7362 McDonald,C.G., Dailey,V.K., Bergstrom,H.C., Wheeler,T.L., Eppolito,A.K., Smith,L.N., Smith,R.F. Periadolescent nicotine administration produces enduring changes in dendritic morphology of medium spiny neurons from nucleus accumbens. Neuroscience Letters 2005; 385(-2-):163-167. >>> Nicotine; Saline; SC; Rat; 1002; 2004; 6 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced at two weeks with the 2004 model; "5.6 mglkglday was chosen to provides plasma nicotine levels comparable to those seen in human smokers." p. 164; bahavioral study.

P7341 Zhou,Y., Chen,R., Catanzaro,S.E., Hu,L.F., Dansky,H.M., Catanzaro,D.F. Differential effects of angiotensin II on atherogenesis at the aortic sinus and descending aorta of apolipoprotein-E-deficient mice. American Journal of Hypertension 2005; 18(-4-):486-492. >>> Angiotensin II; Saline; SC; Mice (knockout); 2004; 8-32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; cardiovascular; peptides.

P7334 Turrone,P., Remington,G., Kapur,S., Nobrega,J.N. Continuous but not intermittent olanzapine infusion induces vacuous chewing movements in rats. Biological Psychiatry 2005; 57(-4-):406-411. >>> Haloperidol; olanzapine; SC; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; comparison of injections vs. mp; long-term study; pumps replaced every four weeks in a new incision; post op. care (placed in heated plexiglass cage); wound clips and vetbond were used to close incision.

P7328 Kharitonenkov,A., Shiyanova,T.L., Koester,A., Ford,A.M., Micanovic,R., Galbreath,E.J., Sandusky,G.E., Hammond,L.J., Moyers,J.S., Owens,R.A., Gromada,J., Brozinick,J.T., Hawkins,E.D., Wroblewski,V.J., Li,D.S., Mehrbod,F., Jaskunas,S.R., Shanafelt,A.B. FGF-21 as a novel metabolic regulator. Journal of Clinical Investigation 2005; 115(-6-):1627-1635. >>> Fibroblast growth factor-21; Saline; SC; Mice; 8 weeks; Controls received mp w/ vehicle; replacement therapy (diabetic, db/db); comparison of SC injections vs. mp (minipump used to study mitogenicity during longer exposure); long-term study.

P7320 Balatsouka,D., Gotfredsen,K., Lindh,C.H., Berglundh,T. The impact of nicotine on bone healing and osseointegration - An experimental study in rabbits. CLINICAL ORAL IMPLANTS RESEARCH 2005; 16(-3-):268-276. >>> Nicotine hydrogen tartrate salt; Water, sterile; SC; Rabbit; 2ML4; 8 weeks; Controls received mp w/ saline; functionality of mp verified by plasma cotinine levels; long-term study; pumps replaced after 4 weeks.

P7300 Venken,K., Boonen,S., Van Herck,E., Vandenput,L., Kumar,N., Sitruk-Ware,R., Sundaram,K., Bouillon,R., Vanderschueren,D. Bone and muscle protective potential of the prostate-sparing synthetic androgen 7alpha-methyl-19-nortestosterone: Evidence from the aged orchidectomized male rat model. Bone 2005; 36(-4-):663-670. >>> Nortestosterone, 7a-methyl-19-; Propandiol; SC; Rat; 2004; 16 weeks; Controls received mp w/ vehicle; replacement therapy (orchidectomy); dose-response (fig. 1); long-term study; pumps replaced every 4 weeks; animal info (male, 13 month old, Wistar, 600-650 g).

P7286 Balatsouka,D., Gotfredsen,K., Lindh,C.H., Berglundh,T. The impact of nicotine on osseointegration - An experimental study in the femur and tibia of rabbits. CLINICAL ORAL IMPLANTS RESEARCH 2005; 16(-4-):389-395. >>> Nicotine hydrogen tartrate salt; water, sterile; SC; Rabbit; 2ML4; 2 months; Controls received mp w/ saline; functionality of mp verified by plasma cotinine levels; long-term study; pumps replaced at 4 weeks; multiple pumps per animal (2); post op. care (streptocillin, temgesic); titanium implants in femur and tibia.

P7245 Mashiko,S., Ishihara,A., Gomori,A., Moriya,R., Ito,M., Iwaasa,H., Matsuda,M., Feng,Y., Shen,Z., Marsh,D.J., Bednarek,M.A., MacNeil,D.J., Kanatani,A. Antiobesity effect of a melanin-concentrating hormone 1 receptor antagonist in diet-induced obese mice. Endocrinology 2005; 146(-7-):3080-3086. >>> Melanin-concentrating hormone 1 receptor antagonist; Propylene glycol; CSF, artificial; CSF/CNS; CSF/CNS (third ventricle); Mice; 1007D; 2002; 2004; 3, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 1 or 4 weeks of vehicle; ALZET brain infusion kit used; peptides; cyanoacrylate adhesive; post op. care (cefamedin); DEXA; cannula placement confirmed by Evans blue dye injection; animal info (C57BL/6); pumps used in lateral ventricle for one group of animals, and third ventricle for others. Third ventricle group used Plastics One cannulae.

P7184 Mathian,A., Weinberg,A., Gallegos,M., Banchereau,J., Koutouzov,S. IFN-alpha induces early lethal lupus in preautoimmune (New Zealand black x New Zealand White)F1 but not in BALB/c mice1. Journal of Immunology 2005; 174(-5-):2499-2506. >>> Interferon, mouse; PBS; BSA; SC; Mice; 1002; 8-10 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 weeks; immunology.

P7168 Kobayashi,Y., Kim,K.J., Itoh,T. Gene expression of bone-resorbing cytokines in rat osteolysis model. JOURNAL OF ORTHOPAEDIC SCIENCE 2005; 10(-1-):62-69. >>> Polyethylene; Serum, rat; Knee (articular cavity); Rat; 2002; 2, 4, 6, 8, 10 weeks; Controls received mp w/ vehicle, comparison of single injection vs. mp, long-term study; pumps replaced every 2 weeks, "Continuous infusion induced more active fibrous tissue formation with osteoclastic bone resorption than did the single injection... even though the dose of PE particles we used for continuous infusion was one order of magnitude lower." (p. 68).

P7070 Foster,HE Jr, Yono,M., Shin,D., Takahashi,W., Pouresmail,M., Afiatpour,P., Latifpour,J. Effects of chronic administration of doxazosin on alpha1-adrenoceptors in the rat prostate. Journal of Urology 2004; 172(-6-):2465-2470. >>> Doxazosin; DMSO; SC; Rat; 8,12 weeks; Controls received mp w/ vehicle; dose-response (table 2); long-term study; cancer (prostatic hyperplasia); 50% DMSO; due to limitations in solubility mp was combined with oral dosing to deliver higher doses.

P7053 Zucker,B., Ludin,D.E., Gerds,T.A., Luecking,C.H., Landwehrmeyer,G.B., Feuerstein,T.J. Gabapentin-lactam, but not gabapentin, reduces protein aggregates and improves motor performance in a transgenic mouse model of Huntington's disease. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 2004; 370(-2-):131-139. >>> Gabapentin-lactam; gabapentin; Isobutanol; saline; SC; Rat; mice (transgenic); 2002; 4-8 weeks; Controls received mp w/ vehicle, or no treatment; functionality of mp verified by residual volume; long-term study; pumps replaced every 2 weeks; no stress (see pg. 137); half-life (pg. 134, 136) approx. 2 hours; "The continuous application of both GBP-L and of GBP by ALZET pumps have maintained the plasma levels so that the actions of these drugs at their targets in the CNS were not interrupted due to their rather short half-lives." (pg. 138); neurodegenerative (huntington's disease).

P7031 Hild,S.A., Attardi,B.J., Reel,J.R. The ability of a gonadotropin-releasing hormone antagonist, acyline, to prevent irreversible infertility induced by the indenopyridine, CDB-4022, in adult male rats: The role of testosterone. Biology of Reproduction 2004; 71(-1-):348-358. >>> Acyline; Tween 80; water, bacteriostatic; SC; Rat; 2002; 2004; 2,4,10,14 weeks; Controls received mp w/ vehicle; dose-response (fig. 2); long-term study; pumps replaced every 4 weeks; peptides; multiple pumps per animal (2); in exp.II two rats died at weeks 14 and 16, both had received mp w/ vehicle weeks -4 to 0, mp removed at week 0; GRH antagonist.

P7005 Duncan,R.E., El-Sohemy,A., Archer,M.C. Mevalonate promotes the growth of tumors derived from human cancer cells in vivo and stimulates proliferation in vitro with enhanced cyclin-dependent kinase-2 activity. Journal of Biological Chemistry 2004; 279(-32-):33079-33084. >>> Mevalonate; SC; Mice (nude); 2004; 13 weeks; Controls received mp w/ vehicle, or isotonic saline; long-term study; pumps replaced every 28 days; cancer (MDA-MB-435); MDA-MB-435 is a highly malignant human cancer of either breast adenocarcinoma or occult melanoma (origin uncertain); cancer (breast, melanoma, adenocarcinoma).

P6870 Ferrandi,M., Molinari,I., Barassi,P., Minotti,E., Bianchi,G., Ferrari,P. Organ hypertrophic signaling within caveolae membrane subdomains triggered by ouabain and antagonized by PST 2238. Journal of Biological Chemistry 2004; 279(-32-):33306-33314. >>> Ouabain; SC; Rat; 2002; 2004; 18 weeks; Controls received mp w/ sterile saline; functionality of mp verified by plasma ouabain concentration; long-term study.

P6838 Thordarson,G., Semaan,S., Low,C., Ochoa,D., Leong,H., Rajkumar,L., Guzman,R.C., Nandi,S., Talamantes,F. Mammary tumorigenesis in growth hormone deficient spontaneous dwarf rats; effects of hormonal treatments. Breast Cancer Research and Treatment 2004; 87(-3-):277-290. >>> Insulin-like growth factor I, recomb. human; SC; Rat; 21 weeks; 10 days; Controls received no treatment; replacement therapy (GH deficient, dwarf); long-term study; cancer (mammary); peptides.

P6822 Lee,J.K., Kim,J.E., Sivula,M., Strittmatter,S.M. Nogo receptor antagonism promotes stroke recovery by enhancing axonal plasticity. Journal of Neuroscience 2004; 24(-27-):6209-6217. >>> Antibody, NgR (310) Ecto-Fc; Immunoglobulin G, rat; PBS; CSF/CNS; Rat; 2ML4; 9 weeks; Controls received mp w/ rat IgG; long-term study; pumps replaced after 28 days; good methods (p. 6210); ALZET brain infusion kit 2 used; peptides; cyanoacrylate adhesive; ischemia (cerebral); Nogo-Nogo receptor (NgR); NgR (310) Ecto-Fc is an IgG fusion protein with a soluble ectodomain fragment of NgG; behavioral study.

P6794 Pittner,R.A., Moore,C.X., Bhavsar,S.P., Gedulin,B.R., Smith,P.A., Jodka,C.M., Parkes,D.G., Paterniti,J.R., Srivastava,V.P., Young,A.A. Effects of PYY[3-36] in rodent models of diabetes and obesity. International Journal of Obesity 2004; 28(-8-):963-971. >>> Peptide YY (3-36); PBS; SC; Rat; mice; 2004; 2ML4; 4, 8 weeks; Controls received mp w/ vehicle; dose-response (fig. 1); comparison of IP injections vs. mp; long-term study; pumps replaced after 4 weeks; diabetes; obesity; peptide.

P6787 Yono,M., Foster,HE Jr, Shin,D., Takahashi,W., Pouresmail,M., Latifpour,J. Doxazosin treatment causes differential alterations of alpha1-adrenoceptor subtypes in the rat kidney, heart and aorta. LIFE SCIENCES 2004; 75(-21-):2605-2614. >>> Doxazosin; DMSO; SC; Rat; 12 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma doxazosin levels; long-term study; antihypertensive; 50% DMSO; doxazosin supplemented orally in drinking water.

P6760 Neves,K.R., Graciolli,F.G., dos Reis,L.M., Pasqualucci,C.A., Moyses,R.M.A., Jorgetti,V. Adverse effects of hyperphosphatemia on myocardial hypertrophy, renal function, and bone in rats with renal failure. Kidney International 2004; 66(-6-):2237-2244. >>> Parathyroid hormone, rat 1-34; Cysteine; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; replacement therapy (parathyroidectomy, 5/6 nephrectomy); long-term study; pumps replaced at day 28.

P6747 Itoh,T., Nagaya,N., Murakami,S., Fujii,T., Iwase,T., Ishibashi-Ueda,H., Yutani,C., Yamagishi,M., Kimura,H., Kangawa,K. C-type natriuretic peptide ameliorates monocrotaline-induced pulmonary hypertension in rats. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 2004; 170(-11-):1204-1211. >>> Natriuretic peptide, C-type; Glucose; SC; Rat; 1,5,8 weeks; Controls received mp w/ vehicle; comparison of IV injections vs. mp; long-term study; pumps replaced every 2 weeks; antihypertensive; peptides; "Continuous infusion of CNP ameliorated MCT - induced pulmonary hypertension and improved survival in rats." (p.1210).

P6742 Ichihara,A., Hayashi,M., Kaneshiro,Y., Suzuki,F., Nakagawa,T., Tada,Y., Koura,Y., Nishiyama,A., Okada,H., Uddin,M.N., Nabi,A.H.M.N., Ishida,Y., Inagami,T., Saruta,T. Inhibition of diabetic nephropathy by a decoy peptide corresponding to the "handle'' region for nonproteolytic activation of prorenin. Journal of Clinical Investigation 2004; 114(-8-):1128-1135. >>> Peptide, prorenin handle region; SC; Rat; 2004; 8, 16, 24 weeks; Controls received mp w/ saline; long-term study; pumps replaced every 28 days; no stress (see pg.1129); enzyme inhibitor (prorenin); peptides; "Continuous infusion of HRP by subcutaneous minipumps did not affect the body weight, BP, or blood glucose levels of either control or diabetic rats during the 24-week treatment period." (p.1129); diabetes.

P6736 Pitkanen,A., Narkilahti,S, Bezvenyuk,Z, Haapalinna,A., Nissinen,J. Atipamezole, an alpha 2-adrenoceptor antagonist, has disease modifying effects on epileptogenesis in rats. Epilepsy Res. 2004; 61(--):119-140. >>> Atipamezole; Saline; SC; Rat; 2ML1; 2ML4; 9 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume and plasma levels; long-term study; pumps replaced every 4 weeks and one week (for the 2ML1); epilepsy; 2ML4 pump implanted, then the 2ML1, then the 2ML4 again; memory; behavioral study.

P6721 Hashimoto,N., Maeshima,Y., Satoh,M., Odawara,M., Sugiyama,H., Kashihara,N., Matsubara,H., Yamasaki,Y., Makino,H. Overexpression of angiotensin type 2 receptor ameliorates glomerular injury in a mouse remnant kidney model. American Journal of Physiology-Renal Physiology 2004; 286(-3-):F516-F525. >>> PD123319; SC; Mice; 2002; 6 weeks; Long-term study; pumps replaced every two weeks; AT2R specific antagonist.

P6708 Huddleston,G.G., Michael,R.P., Zumpe,D., Clancy,A.N. Estradiol in the male rat amygdala facilitates mounting but not ejaculation. PHYSIOLOGY&BEHAVIOR 2003; 79(-2-):239-246. >>> Fadrozole; Saline; SC; Rat; 2004; 8 weeks; Functionality of mp verified by residual volume; long-term study; pumps replaced after 26 days; enzyme inhibitor (aromatase); post op. care (penicillin, banamine); fadrozole is also termed CGS-15949A.

P6602 Tolbert,D.L., Clark,B.R. GDNF and IGF-I trophic factors delay hereditary Purkinje cell degeneration and the progression of gait ataxia. Experimental Neurology 2003; 183(-1-):205-219. >>> Glial-derived neurotrophic factor; insulin-like growth factor I; SC; Rat; 2004; 4,8 weeks; Controls received mp w/ saline; long-term study; pumps replaced at 4 weeks; peptides.

P6541 Brooks,D.E., Kaellberg,M.E., Cannon,R.L., Komaromy,A.M., Ollivier,F.J., Malakhova,O.E., Dawson,W.W., Sherwood,M.B., Kuekuerichkina,E.E., Lambrou,G.X. Functional and structural analysis of the visual system in the rhesus monkey model of optic nerve head ischemia. INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE 2004; 45(-6-):1830-1840. >>> Endothelin; Saline; acetic acid; CSF/CNS (optic nerve); Monkey; 18 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 6 weeks; no stress (see pg. 1833); peptides; "The osmotic minipumps were well tolerated by the monkeys for the length (1.5 years) of the experiment." The long exposure time was used to "mimic more closely the prolonged human glaucomatous condition." p. 1838; pump model not listed; ischemia.

P6528 Sepehrdad,R., Chander,P.N., Singh,G., Stier,CT Jr  American Journal of Physiology-Renal Physiology 2004; 286(-6-):F1185-F1192. >>> Benzamil; dimethylamiloride; Propanediol; Rat; 2002; 8 weeks; long-term study; pumps replaced every 2 weeks.

P6523 Cingolani,O.H., Yang,X.P., Liu,Y.H., Villanueva,M., Rhaleb,N.E., Carretero,O.A. Reduction of cardiac fibrosis decreases systolic performance without affecting diastolic function in hypertensive rats. Hypertension 2004; 43(-5-):1067-1073. >>> Peptide, Ac-SDKP; Acetic acid; SC; Rat; 4 months; Controls received mp w/ vehicle; Long-term study; pumps replaced every 4 weeks; no stress (see pg.1069); cardiovascular; peptides; Ac-SDKP is N-acetyl-seryl-aspartyl-lysyl-proline and is antifibrotic.

P6365 Turrone,P., Remington,G., Kapur,S., Nobrega,J.N. Differential effects of within-day continuous vs transient dopamine D2 receptor occupancy in the development of vacuous chewing movements (VCMs) in rats. Neuropsychopharmacology 2003; 28(-8-):1433-1439. >>> Haloperidol; SC; Rat; 2ML4; 8 weeks; Haloperidol plasma levels taken; comparison of SC injections vs. SC mp; long-term study; pumps replaced every 4 weeks; Vetbond glue and wound clips used to close wounds; "...minipump treated animals had stable haloperidol plasma levels throughout the day...subcutaneously [injection] treated rats experienced a dramatic decline over the same period." p. 1437.

P6302 Robinson,J.J., Aitken,R.P., Atkinson,T, Wallace,J.M., McNeilly,A.S. Effect of continuous infusion of thyrotrophin-releasing hormone on plasma prolactin and on ovarian activity in melatonin-treated ewes. Journal of Reproduction and Fertility 1996; 107(--):17-22. >>> Thyrotropin-rel. factor; SC; Sheep; 2ML4; 3 months; Long-term study; new pumps implanted every 28 days; multiple pumps per animal (3).

P6255 Moulder,J.E., Fish,B.L., Cohen,E.P. Impact of angiotensin II type 2 receptor blockade on experimental radiation nephropathy. RADIATION RESEARCH 2004; 161(-3-):312-317. >>> PD123319; Saline; SC; Rat; 2ML4; 12 weeks; Comparison of SC injections vs. mp; long-term study; pumps replaced every 4 weeks; PD123319 is an AT2 blocker.

P6220 Pizzo,D.P., Winkler,J., Sidiqi,I., Waite,J.J., Thal,L.J. Modulation of sensory inputs and ectopic presence of Schwann cells depend upon the route and duration of nerve growth factor administration. Experimental Neurology 2002; 178(-1-):91-103. >>> Nerve growth factor, recomb. human; cytochrome c; PBS; CSF/CNS; CSF/CNS (nucleus basalis magnocellularis); Rat; 2004; 3,6 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; stress/adverse reaction: (see pg. 98) minor inflammatory response at cannulation site; brain tissue distribution; peptides.

P6100 Feeney,S.J., Austin,L., Bennett,T.M., Kurek,J.B., Jean-Francois,M.J.B., Muldoon,C., Byrne,E. The effect of leukaemia inhibitory factor on SOD1 G93A murine amyotrophic lateral sclerosis. Cytokine 2003; 23(-4-5-):108-118. >>> Leukemia inhibitory factor; CSF/CNS (intrathecal); Mice (transgenic); 2004; 13 weeks; Comparison of SC injections vs. IT mp; long-term study; pumps replaced every 4 weeks; ALZET brain infusion kit 1 used (per Dr. Feeney); used the BIK for the IT infusion; glued and sutured the cannula in place; "All mice that entered the study recovered from the surgical procedure and appeared to have no adverse effects." (p. 111); neurodegenerative (Amyotrophic Lateral Sclerosis).

P6082 Hayashi,T., Sohmiya,K., Ukimura,A., Endoh,S., Mori,T., Shimomura,H., Okabe,M., Terasaki,F., Kitaura,Y. Angiotensin II receptor blockade prevents microangiopathy and preserves diastolic function in the diabetic rat heart. HEART 2003; 89(-10-):1236-1242. >>> Candesartan; IP; Rat; 2002; 6 weeks; Long-term study; pumps replaced every two weeks; diabetes.

P5937 Simon,G., Jackel,M., Illyes,G. Role of angiotensin II, sympathetic stimulation and salt in the development of structural vascular changes in rat kidney. Clinical and Experimental Pharmacology and Physiology 2003; 30(-7-):476-481. >>> Angiotensin II; SC; Rat; 2ML4; 4,12 weeks; Blood pressure taken; long-term study; pumps replaced every 28 days for 12 week study; cardiovascular; peptides.

P5936 Liu,R.L., Liu,I.Y., Bi,X.N., Thompson,R.F., Doctrow,S.R., Malfroy,B., Baudry,M. Reversal of age-related learning deficits and brain oxidative stress in mice with superoxide dismutase/catalase mimetics. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES O F AMERICA 2003; 100(-14-):8526-8531. >>> Euk-189; euk-207; Mannitol; water; SC; Mice; 2004; 3 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 28 days in same location; pumps primed for >40 hours in 5% mannitol; eukarion is a synthetic catalytic scavenger of reactive oxygen species; stress/adverse reaction: in 10% of mice, the pumps were replaced on the other side of hip area due to skin damage of original site of implantation; behavioral study.

P5890 Gregorevic,P., Plant,D.R., Leeding,K.S., Bach,L.A., Lynch,G.S. Improved contractile function of the mdx dystrophic mouse diaphragm muscle after insulin-like growth factor-I administration. American Journal of Pathology 2002; 161(-6-):2263-2272. >>> Insulin-like growth factor; HCL; Saline; SC; Mice; 1002; 8 weeks; Functionality of mp verified by residual volume; long-term study; pumps replaced after 2 weeks; no stress (see pg. 2264); good methods p. 2264; muscular dystrophy; partially dipped in paraffin wax to achieve a pumping rate of 0.125 ul/hr for 28 days.

P5726 Eder,I.E, Hoffmann,J, Rogatsch,H, Schafer,G, Zopf,D, Bartsch,G, Klocker,H  Cancer Gene Therapy 2003; 9(-2-):117-125. >>> Oligonucleotide, antisense; Water, distilled; SC; Mice (nude); 7 weeks; Controls received mp w/ vehicle; long-term study, pumps replaced every two weeks; no stress (see pg. 122); cancer (prostate); antisense; pumps implanted SC in the vicinity of tumors; ODN w/ specificity to the androgen receptor; "...mice did not show any reaction on the subcutaneously installed ALZET diffusion pumps. These were chosen to guarantee a continuous systemic administration of oligonucleotides, a commonly used method for antisense treatment in vivo." (p 122).

P5330 Ramchandra,R., Barrett,C.J., Guild,S.J., Malpas,S.C. Neural control of the renal vasculature in angiotensin II-induced hypertension. Clinical and Experimental Pharmacology and Physiology 2002; 29(-10-):867-872. >>> Angiotensin II; IV (jugular); Rabbit; 2ML4; 7 weeks; Long-term study, pumps replaced after 4 weeks; cardiovascular.

P5237 Abrahamsen,C.T., Barone,F.C., Campbell,W.G.,Jr., Nelson,A.H., Contino,L.C., Pullen,M.A., Grygielko,E.T., Edwards,R.M., Laping,N.J., Brooks,D.P. The angiotensin type 1 receptor antagonist, eprosartan, attenuates the progression of renal disease in spontaneously hypertensive stroke-prone rats with accelerated hypertension. J Pharmacol Exp.Ther. 2002; 301(-1-):21-28. >>> Eprosartan; IP; Rat; 2ML4; 12 weeks; Controls received mp w/ vehicle; long-term study. pumps replaced every 28 days; cardiovascular; antihypertensive; angiotensin type 1 receptor antagonist; ischemia.

P5164 Wang,X., Abdel-Rahman,A.A. Estrogen modulation of eNOS activity and its association with caveolin-3 and calmodulin in rat hearts. American Journal of Physiology-Heart and Circulatory Physiology 2002; 282(--):H2309-H2315. >>> Estradiol, 17B-; Saline; SC; rat; 2004; 12 weeks; controls received mp w/ vehicle; functionality of mp verified by plasma estradiol levels; replacement therapy (ovariectomy); long-term study, pumps replaced every 4 wks; cardiovascular.

P5100 Loot,A.E., Roks,A.J., Henning,R.H., Tio,R.A., Suurmeijer,A.J., Boomsma,F., van Gilst,W.H. Angiotensin-(1-7) attenuates the development of heart failure after myocardial infarction in rats. Circulation 2002/4/2; 105(-13-):1548-1550. >>> Angiotensin-(1-7); Saline; IV; rat; 2004; 8 weeks; controls received mp w/ vehicle; functionality of mp verified by plasma Ang(1-7) levels; long-term study, pumps replaced after 4 weeks; cardiovascular; peptides.

P5097 Garverick,H.A., Baxter,G., Gong,J., Armstrong,D.G., Campbell,B.K., Gutierrez,C.G., Webb,R. Regulation of expression of ovarian mRNA encoding steroidogenic enzymes and gonadotrophin receptors by FSH and GH in hypogonadotrophic cattle. REPRODUCTION 2002; 123(--):651-661. >>> Aldosterone; Saline; SC; rat; 2002; 7.5 weeks; controls received mp w/ vehicle; functionality of mp verified by systolic BP measurement; dose response (graph p. 1446); long-term study, pumps replaced every 2 weeks; cardiovascular; mineralocorticoid hypertension;.

P4945 Rothermund,L., Pinto,Y.M., Vetter,R., Herfort,N., Kossmehl,P., Neumayer,H.H., Paul,M., Kreutz,R. Effects of angiotensin II subtype 1 receptor blockade on cardiac fibrosis and sarcoplasmic reticulum Ca2+ handling in hypertensive transgenic rats overexpressing the Ren2 gene. Journal of Hypertension 2001; 19(--):1465-1472. >>> Eprosartan; Saline; IP; rat; 2ML4; 20 weeks; Controls received mp w/ vehicle; long-term study, pumps replaced every 4 weeks; cardiovascular; antihypertensive; Eprosartan is an Angiotensin II Type 1 receptor antagonist, given at antihypertensive (6 mg/kg/day) or hypertensive (60 mg/kg/day) doses..

P4904 Halonen,T., Nissinen,J., Pitkanen,A. Chronic elevation of brain GABA levels beginning two days after status epilepticus does not prevent epileptogenesis in rats. Neuropharmacology 2001; 40(--):536-550. >>> Vigabatrin; Saline; SC; rat; 2ML2; 10 weeks; controls received mp w/ vehicle; long-term study, pumps replaced every 2 weeks for 10 weeks; epilepsy; seizures..

P4846 Iwaniec,U.T., Fung,Y.K., Akhter,M.P., Haven,M.C., Nespor,S., Haynatzki,G.R., Cullen,D.M. Effects of nicotine on bone mass, turnover, and strength in adult female rats. Calcified Tissue International 2001; 68(--):358-364. >>> Nicotine; Saline; SC; rat; 2004; 3 months; controls received mp w/ vehicle; functionality of mp verified by serum nicotine levels; dose response (graphs p. 360); long-term study, pumps replaced every 30 days..

P4803 Fang,T.-C., Wu,C.C., Huang,W.C. Inhibition of nitric oxide synthesis accentuates blood pressure elevation in hyperinsulinemic rats.. Journal of Hypertension 2001; 19(-7-):1255-1262. >>> Insulin, porcine;; Glycerin; Ethanol;; SC;; rat;; 2002;; 6 weeks;; Controls received mp w/ vehicle; functionality of mp verified by removing, measuring residual volume; long-term study, pumps replaced after 14 days and 28 days; vehicle was 50:50 glycerin, ethanol mix;.

P4775 Weiss,D., Kools,J.J., Taylor,W.R. Angiotensin II-induced hypertension accelerates the development of atherosclerosis in ApoE-deficient mice. Circulation 2001; 103(--):448-454. >>> Angiotensin II; Norepinephrine;; Water; Sodium chloride; Acetic acid;; SC;; mice;; 1002;; 4 or 8 weeks; Functionality of mp verified by plasma angiotensin levels; long-term study, pumps replaced 4 to 8 week study; vehicle solution was 0.15 M NaCl, .01 N acetic acid;.

P4748 Iwaniec,U.T, Fung,Y.K., Cullen,D.M, Akhter,M.P, Haven,M.C, Schmid,M  Calcif.Tissue Int. 2000; 67(--):68-74. >>> Nicotine tartrate;; Saline;; SC;; rat;; 2004;; 2-3 months;; Controls received mp w/ vehicle; functionality of mp verified by serum nicotine levels via gas chromatography; long-term study, pumps replaced every 30 days; low (3.0 mg/kg.day) or high dose (4.5 mg/kg.day); bone density strength;.

P4742 Tang,W, Seidman,M.D, Henig,J.P, Shulman,A, Stracher,A  International Tinnitus Journal 2001; 7(-1-):4-12. >>> Leupeptin;; Hank's solution;; ear (round window);; guinea-pig;; 2002;; 8 weeks;; Controls received mp w/ vehicle; tissue perfusion (bulla); long-term study, pumps replaced every 13 days; IntraEAR catheter used; Leupeptin is a potent calpain inhibitor; neuroprotection; buprenorphine given as postoperative analgesic; chloramphenicol given as prophlactic antibiotic;.

P4680 Hieda,Y, Keyler,D.E, Ennifar,S, Fattom,A, Pentel,P.R  International Journal of Immunopharmacology 2000; 22(--):809-819. >>> Nicotine bitartrate;; Saline;; SC;; rat;; 2ML4;; 11 weeks;; Controls received mp w/ vehicle; comparison of chronic bolus injections vs. mp; long-term study, pumps replaced after 4 and 8 weeks; immunology; dependence;.

P4660 Cooper,T.T, Clancy,A.N., Karom,M, Moore,T.O, Albers,H.E. Conversion of testosterone to estradiol may not be necessary for the expression of mating behavior in male Syrian hamsters.. Hormones and Behavior 2000; 37(--):237-245. >>> Fadrozole;; Saline;; SC;; hamster;; 2004; 2002;; 8 weeks;; Controls received empty mp; functionality of mp verified by weighing residual fluid; long-term study, pumps replaced every 2 or 4 weeks; enzyme inhibitor (nonsteroidal aromatase inhibitor);.

P4643 Cummings,D.E, Kumar,N., Bardin,C.W., Sundaram,K., Bremner,W.J. Prostate-sparing effects in primates of the potent androgen 7a-Methyl-19-Nortestosterone: A potential alternative testosterone for androgen replacement and male contraception.. Journal of Clinical Endocrinology and Metabolism 1998; 83(-12-):4212-4219. >>> Nortestosterone, 7a-methyl-19-; Testosterone acetate;; Cyclodextrin, B-;; SC;; monkey;; 20 weeks;; functionality of mp verified by hormone serum levels; replacement therapy (castration); dose-response (p.4213); long-term study, pumps replaced weekly during 1st treatment, bi-weekly during 2nd period,&every 4 weeks during the last 2 periods; both steroids were dissolved in 45% solution of 2-hydroxypropyl-B-cyclodextrin; MRI.

P4631 Fung,Y.K., Mendlik,M.G, Haven,M.C, Akhter,M.P, Kimmel,D.B  Pharmacology and Toxicology 1998; 82(--):243-249. >>> Nicotine tartrate;; Saline, sterile;; SC;; rat;; 2004;; 2 months;; controls received mp w/ saline; long-term study, pumps replaced after 30 days; toxicology; post op. care (incision covered w/ mycitracin antibiotic and lidocaine).

P4528 Maruno,K, Absood,A, Said,S.I  Proc.Natl.Acad.Sci. 1998/11; 95(--):14373-14378. >>> Vasoactive intestinal peptide; forskolin; PBS; DMSO; SC; Mice (nude); 2002; 8 weeks; Controls received mp w/PBS; dose-response (pg. 14376-14377); long-term study, pumps replaced after 2 weeks; cancer (lung); multiple pumps per animal (2) in some animals; "For systemic delivery of peptides in vivo, constant infusion by osmotic pumps increases efficacy and reduces side effects relative to bolus injection." (p. 14377)..

P4506 Fang,T-C, Huang,W-C  Hypertension 1998; 32(--):235-242. >>> Insulin; PD123319; Losartan;; Glycerin; Ethanol; Saline;; SC;; rat;; 2002;; 6 weeks;; controls received mp w/vehicle; functionality of mp verified by residual volume; long-term study, pump replaced every 14 days; peptides; cardiovascular; insulin given singly or concomitantly w/losartan or PD 123319 from separate pumps; penicillin given at time of surgery..

P4501 Huang,W-C, Fang,T-C, Cheng,J-T  Hypertension 1998/8; 32(--):249-254. >>> Insulin;; Glycerin; Ethanol;; SC;; rat;; 2002;; 6 weeks;; controls received mp w/vehicle; functionality of mp verified by residual volume; long-term study, pumps replaced every 14 days; peptides; cardiovascular;.

P4480 Ueda,Y, Kitakaze,M, Imakita,M, Ishibashi-Ueda,H, Minamino,T, Asanuma,H, Ozaki,T, Imamura,E, Kuzuya,T, Hori,M  Arterioscler.Thromb.Vasc.Biol. 1999; 19(--):343-347. >>> FK-633; SC;; dog;; 2ML4;; 3 months;; controls received mp w/ vehicle; long-term study, pump replaced after 1 and 2 months; no stress (p. 344); cardiovascular;.

P4466 Fehniger,T.A, Carson,W.E, Mrózek,E, Caligiuri,M.A  Blood 1997/11/1; 90(-9-):3647-3653. >>> Interleukin-2; PBS; Serum, syngeneic mouse; SC; Mice; 8 weeks; Controls received mp w/vehicle; functionality of mp verified by plasma levels; long-term study, pump replaced every 10 days; immunology; peptides.

P4455 MacLaughlin,M, Monserrat,A.J, Muller,A, Matoso,M, Amorena,C  Kidney Blood Press Res. 1998; 21(--):329-334. >>> Hoe 140; Saline, physiological;; SC;; rat;; 2002;; 14 weeks;; controls received mp w/vehicle; functionality of mp verified by plasma levels; long-term study, pumps replaced every other week;.

P4435 Ferrari,P, Torielli,L, Ferrandi,M, Padoani,G, Duzzi,L, Florio,M, Conti,F, Melloni,P, Vesci,L, Corsico,N, Bianchi,G. PST 2238: A new antihypertensive compound that antagonizes the long-term pressor effect of ouabain. The Journal of Pharmacology and Experimental Therapeutics. 1998; 285(--):83-94. >>> Ouabain;; Saline;; rat;; 2004; 2002;; 4, 8 weeks;; controls received mp w/saline; functionality of mp verified by ouabain plasma levels and blood pressure increase; long-term study, pump replaced every 15 days for model 2002 and every 28 days for model 2004;.

P4413 Yang,H.T, Deschenes,M.R, Ogilvie,R.W, Terjung,R.L  Circulation Research 1996/7; 79(-1-):62-69. >>> Fibroblast Growth Factor, basic; Heparin;; Saline; Glycerol; Sodium azide;; IA (Femoral);; rat;; 2002;; 1,2,4 weeks;; controls received mp w/ heparin; functionality of mp verified by residual volume; long-term study, pumps replaced at day 14; no stress (p. 63); good methods (p.63); peptides; cardiovascular;.

P4366 Day-Lollini,P.A., Stewart,G.R., Taylor,M.J., Johnson,R.M., Chellman,G.J. Hyperplastic changes within the leptomeninges of the rat and monkey in response to chronic intracerebroventricular infusion of nerve growth factor. Exp.Neurol. 1997/5; 145(-1-):24-37. >>> Nerve growth factor;; Saline; Citric acid; Albumin, rat; Albumin, human;; CSF/CNS;; rat; monkey;; 2002; 2ML4;; 6 months;; controls received mp with vehicle; functionality of mp verified by residual volume; dose-response; long-term study, pumps replaced every 28 days; stability of NGF verified at 37ºfor 1 month by RP-HPLC, bioassay, and/or ELISA; good methods; peptides; recomb. human NGF used; half the mp was coated with paraffin to reduce flow rate; NGF solutions were placed in Lynch coiled tubing rather than pumps, due to adsorptive loss of rhNGF to the pump reservoir; in monkeys, ventricular cannula placement was confirmed by rapid drop in fluid level in PE tubing;.

P4351 Cioffi,G.A., Sullivan,P. The effect of chronic ischemia on the primate optic nerve. European Journal of Ophthalmology 1999; 9(-Supp. 1-):S34-S36. >>> Endothelin-I; CSF/CNS (optic nerve);; monkey; 2-6 months;; long-term study, pumps replaced after 4 weeks; peptides; abstract states pump implanted in rabbits also but no mention of rabbits throughout paper; ischemia.

P4343 Pitkanen,A., Nissinen,J., Jolkkonen,E., Tuunanen,J., Halonen,T. Effects of vigabatrin treatment on status epilepticus-induced neuronal damage and mossy fiber sprouting in the rat hippocampus. Epilepsy Research 1999; 33(--):67-85. >>> Vigabatrin;; Saline;; SC;; rat;; 2ML1;; 2 months;; controls received mp with vehicle; functionality of mp verified by plasma levels and residual volume; long-term study, pumps replaced every 2 weeks; enzyme inhibitor;.

P4329 Winkler,J., Ramirez,G.A., Kuhn,H.G., Peterson,D.A., Day-Lollini,P.A., Stewart,G.R., Tuszynski,M.H., Gage,F.H., Thal,L.J. Reversible schwann cell hyperplasia and sprouting of sensory and sympathetic neurites after intraventricular administration of nerve growth factor. Annals of Neurology 1997; 41(-1-):82-93. >>> Nerve growth factor; Cytochrome C;; CSF, artificial; Albumin, rat serum; Gentamycin;; CSF/CNS;; rat;; 2002;; 7 weeks;; long-term study, pumps replaced after 3.5 weeks; peptides; recomb. human NGF used; pumps coated with paraffin to reduce flow rate;.

P4326 Tyml,K., Mathieu-Costello,O., Cheng,L., Noble,E.G. Differential microvascular response to disuse in rat hindlimb skeletal muscles. J.Appl.Physiol. 1999; 87(-4-):1496-1505. >>> Tetrodotoxin;; Saline;; CSF/CNS (sciatic nerve);; rat;; 2002;; 2, 8 weeks;; controls received no treatment; tissue perfusion (nerve cuff); long-term study, pumps replaced every 2 weeks;.

P4298 Kim,K.J., Kobayashi,Y., Itoh,T. Osteolysis model with continuous infusion of polyethylene particles. Clinical Orthopaedics and Related Research 1998; (-352-):46-52. >>> Polyethylene particles; Serum, rat;; knee (articular cavity); rat;; 4-10 weeks;; controls received mp w/ vehicle; tissue perfusion (joint knee cavity); long-term study, pumps replaced every 2 weeks; diagram of model (p. 47); "In the clinical setting, wear debris are generated continuously... (T)herefore, there is a need...(for)..an in vivo model...(for) continuous infusion of wear particles" (pp. 46-47); filling tube was implanted into knee joint cavity;.

P4232 Andreassen,O.A., Ferrante,R.J., Beal,M.F., Jorgensen,H.A. Oral dyskinesias and striatal lesions in rats after long-term co-treatment with haloperidol and 3-nitropropionic acid. Neuroscience 1998; 87(-3-):639-648. >>> Nitropropionic acid, 3-; Water; NaOH; SC; rat; 16 weeks; controls received empty plastic tube; long-term study, pumps replaced after 33 and 38 days; stress/adverse reaction (p. 640); enzyme inhibitor.

P4160 Turner,N.D., Byers,F.M., Kenison,D.C. Plasma kinetics of zeranol in steers receiving variable doses in implant or mini-osmotic pump form. Unknown 1999; (--):71-75. >>> Zeranol; Water; cattle (steer); 56 days; comparison of pellets vs. mp; long-term study, pumps replaced after 28 days.

P4125 Winkler,J., Power,A.E., Ramirez,G.A., Thal,L.J. Short-term and complete reversal of NGF effects in rats with lesions of the nucleus basalis magnocellularis. Brain Research 1998; 788(--):1-12. >>> Nerve growth factor; Cytochrome C; Gentamicin; CSF, artificial; Albumin, rat serum; CSF/CNS; rat; 2002; 7 weeks; long-term study, pumps replaced after 3.5 weeks; "the pumps were paraffin-coated in 13 mm of wax, resulting in a flow rate of 0.25 ul/h (p. 2); recomb. human NGF used; peptides.

P4059 Webb,R.L., Barclay,B.W., Navarrete,A.E., Wosu,N.J., Sahota,P. Protective effects of valsartan and benazeprilat in salt-loaded stroke-prone spontaneously hypertensive rats. Clin.Exp.Hypertens. 1998; 20(-7-):775-793. >>> Valsartan; Benazeprilat; NaOH; SC; rat; 2ML2; 9.5 weeks; controls received mp w/vehicle; long-term study, pumps replaced every 2 weeks; radiotelemetric devices implanted concomitantly for blood pressure and heart rate measurement; antihypertensive; cardiovascular; ischemia (cerebral).

P4037 Gasslander,T., Mukaida,H., Herrington,M.K., Hinder,R.A., Adrian,T.E. Profound duodenogastric reflux causes pancreatic growth in rats. Gut 1995; 36(--):137-141. >>> Devazepine; DMSO; SC; rat; 2002; 6 weeks; devazepine (also called MK309) is a CCK receptor antagonist; controls received silicone tube or sham surgery; long-term study, pumps replaced after 14 days; peptides.

P4030 Rao,A.J., Ramachandra,S.G., Ramesh,V., Krishnamurthy,H.N., Jayaraman,S., Gopalakrishnan,K., Juneja,H.S. Effect of chronic administration of tamoxifen on fertility in male bonnet monkeys (macaca radiata). Andrologia 1998; 30(--):129-132. >>> Tamoxifen; Ethanol; Propylene glycol; SC; monkey; 90 days; controls received mp w/vehicle; long-term study, pumps replaced every 30 days.

P3979 Hashimoto,Y., Kurosawa,Y., Minami,K., Fushimi,K., Narita,H. A novel angiotensin II-receptor antagonist, 606A, induces regression of cardiac hypertrophy, augments endothelium-dependent relaxation and improves renal function in stroke-prone spontaneously hypertensive rats. Jpn.J.Pharmacol. 1998; 76(--):185-192. >>> 606A; Saline; SC; rat; 2ML4; 8 weeks; controls received mp w/saline; long-term study, pumps replaced after 4 weeks; cardiovascular; 606A is an angiotensin AT1 receptor antagonist.

P3967 Wheeler,S.E., Fitzgerald,B.P. Prolactin concentrations are not suppressed in mares administered constant exogenous melatonin. J.Equine Vet.Sci. 1998; 18(-1-):44-47. >>> Melatonin; Saline; DMSO; SC; horse; 12 weeks; controls received mp w/vehicle; functionality of mp verified by serum assays on days 5, 26 and 59; long-term study, pumps replaced every 28 days.

P3953 Silcox,D.H., Boden,S.D., Schimandle,J.H., Johnson,P., Whitesides,T.E., Hutton,W.C. Reversing the inhibitory effect of nicotine on spinal fusion using an osteoinductive protein extract. Spine 1998; 23(-3-):291-297. >>> Nicotine; SC; rabbit; 2ML4; 5, 10 weeks; long-term study, pumps replaced every 17 days; no stress (see pg. 293); stress/adverse reaction: wound infection (pg. 293).

P3913 Shibayama,M., Hattori,S., Himes,B.T., Murray,M., Tessler,A. Neurotrophin-3 prevents death of axotomized Clarke's nucleus neurons in adult rat. J.Comp.Neurol. 1998; 390(--):102-111. >>> NT-3; CSF/CNS (spinal cord, intrathecal); rat; 2ML4; 2 months; controls received mp w/PBS; functionality of mp verified by aspirating residual volume; comparison of gel foam vs. mp; long-term study, pumps replaced after 4 weeks; stability verified by bioactivity assay; peptides.

P3836 Nakamura,T. Contribution of L-arginine-derived nitric oxide to the structural integrity of coronary artery and cardiac interstitium, and to the regulation of sympathetic nerve activity: assessment using a rat model produced by chronic nitric oxide inhibition. Hokkaido J.Med.Sci. 1997; 72(-5-):503-516. >>> L-NAME; IP; rat; 2ML4; 4,8 weeks; controls received mp w/saline; dose-response; long-term study, pumps replaced after 4 weeks; cardiovascular.

P3823 Huang,Y., Snyder,R., Kligshteyn,M., Wickstrom,E. Prevention of tumor formation in a mouse model of burkitt's lymphoma by 6 weeks of treatment with anti-c-myc DNA phosphorothioate. Molec.Medicine 1995; 1(-6-):647-658. >>> Oligodeoxynucleotide, phosphorothioate antisense; Saline; SC; mice; 2002; 6 weeks; Controls received mp w/saline or scrambled DNA; comparison of pellets and SC injections vs. mp; long-term study, pumps replaced every 2 weeks; "Total amount of DNA in the pellets and the rate of release from each pellet varied widely and unpredictably." (pg. 649); cancer; toxicology; antisense.

P3775 Robinson,S.E., Maher,J.R., Wallace,M.J., Kunko,P.M. Perinatal methadone exposure affects dopamine, norepinephrine, and serotonin in the weanling rat. Neurotoxicol.Teratol. 1997; 19(-4-):295-303. >>> Methadone HCl; SC; rat (pregnant); 42 days; controls received mp w/sterile water; dependence; long-term study, pumps replaced after 4 weeks; teratology.

P3746 Walduck,A.K., Opdebeeck,J.P. Effect of the profile of antigen delivery on antibody responses in mice. J.Controlled Release 1997; 43(--):75-80. >>> BSA; PBS; SC; mice; 2002; 4, 8, 12 weeks; controls received PBS injections; comparison of sc injections vs. mp; long-term study, pumps replaced every 2 weeks; immunology.

P3737 Keller,D.A., Marshall,C.E., Lee,K.P. Subchronic nasal toxicity of hexamethylphosphoramide administered to rats orally for 90 days. Fundam.Appl.Toxicol. 1997; 40(--):15-29. >>> Hexamethylphosphoramide; Water; SC; rat; 2ML4; 45, 85 days; comparison of oral dosings vs. mp; long-term study, pumps replaced every 28 days; stability verified by gas chromatography; ALZAID chemical compatibility kit used to test compatibility of agent and vehicle (25% hexamethylphosphoramide); toxicology.

P3706 Basile,A.S., Huang,J.-M., Xie,C., Webster,P., Berlin,C., Skolnick,P. N-Methyl-D-aspartate antagonists limit aminoglycoside antibiotic-induced hearing loss. Nature Medicine 1996; 2(-12-):1338-1343. >>> MK-801; Ifenprodil tartrate; DMSO; Water, deionized; Alkamuls EL620; SC; guinea-pig; 2002; no duration posted; long-term study, pumps replaced after 14 days; agents NMDA antagonists; 40% DMSO used; dizocilpine maleate used.

P3692 Gong,J.G., Campbell,B.K., Bramley,T.A., Gutierrez,C.G., Peters,A.R., Webb,R. Suppression in the secretion of follicle-stimulating hormone and luteinizing hormone, and ovarian follicle development in heifers continuously infused with a gonadotropin-releasing hormone agonist. Biol.Reprod. 1996; 55(--):68-74. >>> Buserelin; SC; cattle; 2ML4; 48 days; controls received no treatment; long-term study, pumps replaced after 28 days; agent is a GnRH agonist; pumps replaced in opposite shoulder than original placement; researchers compare mp w/ injections from previous study (p. 72).

P3620 Malpas,S.C., Groom,A.S., Head,G.A. Baroreflex control of heart rate and cardiac hypertrophy in angiotensin II-induced hypertension in rabbits. Hypertension 1997; 29(--):1284-1290. >>> Angiotensin II; IV (jugular); rabbit; 2ML4; 1, 7 weeks; controls received sham pumps; long-term study, pumps replaced at 4 weeks; peptides; cardiovascular.

P3536 Gohlke,P., Kuwer,I., Schnell,A., Mall,G., Unger,T. Blockade of bradykinin b2 receptors prevents the increase in capillary density induced by chronic angiotensin-converting enzyme inhibitor treatment in stroke-prone spontaneously hypertensive rats. Hypertension 1997; 29(-2-):478-482. >>> Hoe 140; Saline; SC; rat; 14 weeks; controls received mp w/ saline; long-term study, pumps replaced every 2 weeks; cardiovascular; Hoe 140 is icatibant.

P3475 Orgul,S., Cioffi,G.A., Wilson,D.J., Bacon,D.R., Van Buskirk,E.M. An endothelin-1 induced model of optic nerve ischemia in the rabbit. Invest.Ophthalmol.Vis.Sci. 1996; 37(-9-):1860-1869. >>> Endothelin-1; CSF/CNS (optic nerve); rabbit; 2, 8 weeks; controls received mp w/ balanced salt solution; tissue perfusion (perineural region of optic nerve); long-term study, pumps replaced every 2 weeks; peptides; pump implanted above the eye; ischemia (optic nerve).

P3374 Madeddu,P., Anania,V., Parpaglia,P.P., Demontis,M.P., Varoni,M.V., Fattaccio,M.C., Glorioso,N. Chronic kinin receptor blockade induces hypertension in deoxycorticosterone-treated rats. Br.J.Pharmacol. 1993; 108(--):651-657. >>> Hoe 140; Saline, physiological; SC; rat; 2ML4; 6 weeks; controls received mp w/saline; long-term study, pumps replaced after 28 days; agent bradykinin antagonist; author states, "...osmotic pump implantation might have amplified the functional importance of B1 receptors&stimulated the generation of T-kinin" (p. 655) but concludes that this is unlikely.

P3283 McLaughlin,C.L., Rogan,G.J., Buonomo,F.C., Cole,W.J., Hartnell,G.F., Hudson,S., Kasser,T.R., Miller,M.A., Baile,C.A. Finishing lamb performance responses to bovine and porcine somatotropins administered by ALZET pumps. J.Anim.Sci. 1991; 69(--):4039-4048. >>> Growth hormone, bovine; Growth hormone, porcine; SC; sheep; 6 weeks; controls received no treatment; functionality of mp verified by in vitro testing; long-term study, pumps replaced every 2 weeks; peptides.

P3266 Zhou,H., Choong,P.C., Kartsogiannis,V., Martin,T.J., Ng,K.W. Transforming growth factor B1 stimulates bone formation and resorption in an in-vivo model in rabbits. Bone 1995; 17(-4 Supp-):443S-448S. >>> Transforming growth factor-B1; HCl; BSA; bone (tibia); rabbit; 2002; 2,3,4,6 weeks; contralateral controls received vehicle; tissue perfusion (bone implant chamber); silicone tubing used; peptides; recomb. human TGF-B1 used; long-term study; good diagram of pump and experimental system (p. 444S).

P3261 Manunta,P., Rogowski,A.C., Hamilton,B.P., Hamlyn,J.M. Ouabain-induced hypertension in the rat: relationships among plasma and tissue ouabain and blood pressure. J.Hypertens. 1994; 12(-5-):549-560. >>> Ouabain octahydrate; PBS, sterile; SC; rat; 2002; 5-6 weeks; controls received mp w/PBS; functionality of mp verified by residual volume; long-term study, pumps replaced every 14 days; "Prolonged infusion may be of physiological relevance because... circulating levels of ouabain do not appear to exhibit large variations in dogs." (p. 557); mp implanted on flank.

P3246 Li,H., Helander,H.F. Parietal cell kinetics after administration of omeprazole and ranitidine in the rat. Scand.J.Gastroenterol. 1995; 30(--):205-209. >>> Ranitidine; Thymidine; 3H tracer; Radio-isotopes; SC; rat; 2002; 2ML1; 5-56 days; controls received mp with saline; long-term study, pumps replaced weekly; 1 ranitidine and 1 thymidine pump used concomitantly in each animal.

P3225 Lyford,G.L., Gutnikov,S.A., Clark,A.M., Rawlins,J.N.P. Determinants of non-spatial working memory deficits in rats given intraventricular infusions of the NMDA antagonist AP5. Neuropsychologia 1993; 31(-10-):1079-1098. >>> AP5, D-; CSF, artificial;; CSF/CNS; rat; 2002; 14-40 days; controls received mp with vehicle; long-term study, pumps replaced every 10 days; sulphonamide powder applied topically to surgical wound.

P3221 Jablonskis,L.T., Howe,P.R.C. Lack of influence of circulating adrenaline on blood pressure in normotensive and hypertensive rats. Blood Pressure 1994; 3(--):112-119. >>> Epinephrine bitartrate; Saline; Ascorbic acid; SC; rat; 2002; 5-6 weeks; long-term study, pumps replaced every 2 weeks; mp implanted in flank region.

P3192 Himura,Y., Felten,S.Y., Kashiki,M., Lewandowski,T.J., Delahanty,J.M., Liang,C.-S. Cardiac noradrenergic nerve terminal abnormalities in dogs with experimental congestive heart failure. Circulation 1993; 88(--):1299-1309. >>> Norepinephrine; SC; dog; 2ML4; 8 weeks; controls received mp with saline; long-term study, pumps replaced after 3-4 weeks.

P3188 Chow,L., de Gasparo,M., Levens,N. Improved glucose metabolism following blockade of angiotensin converting enzyme but not angiotensin AT receptors. Eur.J.Pharmacol. 1995; 282(--):77-86. >>> Benazepril; Valsartan; Water, distilled; NaOH; HCI; IP; rat; 2ML4; 12 weeks; controls received mp with saline; long-term study, pumps replaced every 28 days; valsartan is an angiotensin AT1 receptor antagonist; antihypertensive.

P3164 Holmang,A., Jennische,E., Bjorntorp,P. The effects of long-term hyperinsulinaemia on insulin sensitivity in rats. Acta Physiol.Scand. 1995; 153(--):67-73. >>> Insulin, human; Propranolol; Saline; Ascorbic acid; SC; rat; 2001; 7 weeks; long-term study, pumps replaced weekly; peptides; antihypertensive.

P3142 Uhland-Smith,A., DeLuca,H.F. The necessity for calcium for increased renal vitamin D receptor in response to 1,25-dihydroxyvitamin D. Biochim.Biophys.Acta 1993; 1176(--):321-326. >>> Vitamin D3, 1,25-dihydroxy-; SC; rat; 2002; 4,8 weeks; comparison of oral vitamin D-3 vs. mp; long-term study, pumps replaced after 2 weeks.

P3128 Tilbrook,A.J., Galloway,D.B., Williams,A.H., Clarke,I.J. Treatment of young rams with an agonist of GnRH delays reproductive development. Horm.Behav. 1993; 27(--):5-28. >>> Luteinizing HRH; SC; sheep; 2001; 28 days; 16 weeks; comparison of biocompatible implant vs. mp; long-term study, pumps replaced every 4 weeks; pumps placed in foreleg; pumps more effective than implants in suppressing LH and FSH secretion; D-Trp6-pro9N-ethyl amide-LHRH used.

P3074 Zochodne,D.W., Murray,M., Nag,S., Riopelle,R.J. A segmental chronic pain syndrome in rats associated with intrathecal infusion of NMDA: evidence for selective action in the dorsal horn. Can.J.Neurol.Sci. 1994; 21(--):24-28. >>> NMDA; Magnesium sulfate; PBS; CSF/CNS (spinal cord, intrathecal); rat; 2002; 4-8 weeks; controls received mp with PBS; long-term study, pumps replaced every 2 weeks; NMDA given with MgS04.

P3054 Young,M  Steroids 1995; 60(--):133-136. >>> Aldosterone; SC; rat; 8 weeks; long-term study, pumps replaced at 4 weeks.

P3012 Verhofstad,A.A.J  J.Anat. 1993; 183(--):315-326. >>> Uridine, bromodeoxy-;; PBS;; SC;; rat;; 12 hours to 73 days;; comparison of bolus injections vs mp.; long-term study; no stress (see pg. 323);.

P3000 Dawson,J.M., Essex,C.P., Walsh,A., Beever,D.E., Gill,M., Buttery,P.J. Effect of fish-meal supplementation and B-agonist administration on adipose tissue metabolism in steers given silage. Anim.Prod. 1993; 57(--):397-406. >>> Cimaterol; SC; cattle; 2ML4; 6 weeks; controls received mp w/ buffer; long-term study, pumps replaced every 24 days; cimaterol is a B-adrenergic agonist.

P2998 DiGregorio,G.B., Gonzalez Reyna,A., Murphy,B.D. Roles of melatonin and prolactin in testicular crudescence in mink (Mustela vison). J.Reprod.Fertil. 1994; 102(--):1-5. >>> Prolactin, ovine; SC; mink; 110 days; controls received no treatment; long-term study, pumps replaced every 4 weeks; peptides.

P2984 Evans,D.A.P., Van Der Kleij,A.A.M., Sonnemans,M.A.F., Burbach,J.P.H., Van Leeuwen,F.W. Frameshift mutations at two hotspots in vasopressin transcripts in post-mitotic neurons. Proc.Natl.Acad.Sci.USA 1994; 91(--):6059-6063. >>> Vasopressin; SC; rat; 2ML4; 40 weeks; controls received a tube similar in size to mp and were sham operated every 28 days; replacement therapy (genetic -- homozygous(di/di) -- inability to produce vasopressin); long-term study, pumps replaced every 28 days.

P2975 Sun,Y., Ratajska,A., Zhou,G., Weber,K.T. Angiotensin-converting enzyme and myocardial fibrosis in the rat receiving angiotensin II or aldosterone. J.Lab.Clin.Med. 1993; 122(-4-):395-403. >>> Aldosterone; Angiotensin II; rat; 2,4,6 weeks; controls received no operation or treatment; replacement therapy (nephrectomy); long-term study; peptides.

P2968 Akyurek,L.M., Funa,K., Wanders,A., Larsson,E., Fellstrom,B.C. Inhibition of transplant arteriosclerosis in rat aortic grafts by low molecular weight heparin derivatives. Transplantation 1995; 59(-11-):1517-1524. >>> OAM 71262; LA-heparin; PM 29989; rat; 2ML4; 8 weeks; controls received mp with PM 29989; long-term study, pumps replaced after 4 weeks; stress/adverse reaction (pg. 1519): extreme edema surrounding mp site in >50% of animals with high doses of OAM 71262 or LA-heparin; deficient wound healing seen in 2 animals; agents are heparin derivatives; PM 29989 was used as a control heparin, because its molecular weight and anticoagulant properties are closer to heparin than the other 2 derivatives.

P2951 Sun,Y., Weber,K.T. Angiotensin II and aldosterone receptor binding in rat heart and kidney: response to chronic angiotensin II or aldosterone administration. J.Lab.Clin.Med. 1993; 122(-4-):404-411. >>> Angiotensin II; Aldosterone; rat; 2,4,6,8 weeks; controls received no treatment or surgery; replacement therapy (nephrectomy); long-term study; peptides.

P2924 Ronayne,E., Enright,W.J., Roche,J.F. Effects of continuous administration of gonadotropin-releasing hormone (GnRH) or a potent GnRH analogue on blood luteinizing hormone and testosterone concentrations in prepubertal bulls. Domest.Anim.Endocrinol. 1993; 10(-3-):179-189. >>> Luteinizing HRH; Leuprolide acetate; Saline; SC; cattle; 2ML4; 28,56 days; controls received mp with vehicle; functionality of mp verified by plasma levels; long-term study, pumps replaced at 28 days; peptides.

P2889 Madeddu,P., Parpaglia,P.P., Demontis,M.P., Varoni,M.V., Fattaccio,M.C., Anania,V., Glorioso,N. Early blockade of bradykinin B2-receptors alters the adult cardiovascular phenotype in rats. Hypertension 1995; 25(--):453-459. >>> Hoe 140; Saline, physiological; IP; rat; rat (pregnant); 8 weeks; controls received mp with vehicle; long-term study, pumps replaced.

P2881 Robert,V., Thiem,N.V., Cheav,S.L., Mouas,C., Swynghedauw,B., Delcayre,C. Increased cardiac types I and III collagen mRNAs in aldosterone-salt hypertension. Hypertension 1994; 24(--):30-36. >>> Aldosterone, d-; SC; rat; 8 weeks; controls received mp with serum; long-term study, pumps replaced every 15 days.

P2880 Robinson,J.J., Wallace,J.M., Aitken,R.P., McNeilly,A.S. Effect of chronic treatment with a GnRH agonist to suppress pulsatile LH secretion on the ability of exogenous melatonin to advance oestrous cyclicity in ewes. J.Reprod.Fertil. 1993; 99(--):601-608. >>> Buserelin; SC; sheep; 2ML4; 50 days; long-term study, pumps replaced on day 25; peptides.

P2821 Ataya,K., Pydyn,E., Ramahi-Ataya,A., Orton,C.G. Is radiation-induced ovarian failure in rhesus monkeys preventable by luteinizing hormone-releasing hormone agonists?: preliminary observations. J.Clin.Endocrinol.Metab. 1995; 80(-3-):790-795. >>> Leuprolide acetate; NaCl; SC; monkey; 2ML4; 82 days; controls received mp with vehicle and/or no irradiation; long-term study, pumps replaced every 24 days; leuprolide acetate is a luteinizing hormone-releasing hormone (LHRH) agonist.

P2790 Kohzuki,M., Yasujima,M., Kanazawa,M., Yoshida,K., Fu,L.P., Obara,K., Saito,T., Abe,K. Antihypertensive and renal-protective effects of losartan in streptozotocin diabetic rats. J.Hypertens. 1995; 13(--):97-103. >>> Losartan; Captopril; IP; rat; 2ML4; 8 weeks; controls received mp w/ vehicle; long-term study, pumps replaced; agents infused separately and concomitantly; antihypertensive.

P2784 Wilson,M.E. IGF-I administration advances the decrease in hypersensitivity to oestradiol negative feedback inhibition of serum LH in adolescent female rhesus monkeys. J.Endocrinol. 1995; 145(--):121-130. >>> Insulin-like growth factor I; SC; monkey; 14 months; dose-response (pg.125); long-term study, pumps replaced every 4 weeks; recomb. human IGF-1 used.

P2729 Gohlke,P., Lamberty,V., Kuwer,I., Bartenbach,S., Schnell,A., Linz,W., Scholkens,B.A., Wiemer,G., Unger,T. Long-term low-dose angiotensin converting enzyme inhibitor treatment increases vascular cyclic guanosine 3',5'-monophosphate. Hypertension 1993; 22(-5-):682-687. >>> Hoe 140; Sodium chloride; SC; rat; 14 weeks; controls received mp w/ vehicle; long-term study, pumps replaced every 2 weeks.

P2681 Campbell,S.E., Janicki,J.S., Matsubara,B.B., Weber,K.T. Myocardial fibrosis in the rat with mineralocorticoid excess; prevention of scarring by amiloride. Am.J.Hypertens. 1993; 6(-6-):487-495. >>> Aldosterone; Amiloride; PEG; Water, double-distilled; Ethanol; SC; rat; 2002; 8 weeks; controls received mp w/ vehicle; long-term study, pumps replaced every 2 weeks; agents given separately and together.

P2662 Brilla,C.G., Matsubara,L.S., Weber,K.T. Anti-aldosterone treatment and the prevention of myocardial fibrosis in primary and secondary hyperaldosteronism. J.Mol.Cell.Cardiology 1993; 25(--):563-575. >>> Aldosterone; SC; rat; 2002; 8 weeks; controls were unoperated, untreated rats; functionality of mp verified by plasma levels; long-term study.

P2646 Brooks,J., McNeilly,A.S. Regulation of gonadotrophin-releasing hormone receptor mRNA expression in the sheep. J.Endocrinol. 1994; 143(--):175-182. >>> Buserelin; SC; sheep; 2ML4; 42 days; long-term study, pumps replaced after 26 days; peptides; pumps inserted in the flank; buserelin is a synthetic GnRH agonist, [D-Ser(But)] GnRH(1-9) nonapeptide-ethylamide.

P2606 Ataya,K., Ramahi-Ataya,A. Reproductive performance of female rats treated with cyclophosphamide and/or LHRH agonist. Reprod.Toxicol. 1993; 7(-3-):229-235. >>> Leuprolide; Sodium chloride; SC; rat; 2002; 76-98 days; controls received mp w/ saline and/or sham operations; long-term study, pumps replaced every 12 days; cancer; toxicology.

P2541 Pinski,J., Halmos,G., Szepeshazi,K., Schally,A.V. Antagonists of bombesin/gastrin-releasing peptide as adjuncts to agonists of luteinizing hormone-releasing hormone in the treatment of experimental prostate cancer. Cancer 1993; 72(-11-):3263-3270. >>> RC-3095; Luteinizing HRH; Propylene glycol; Water; SC; rat; 2ML4; 7 weeks; controls received injection of vehicle only; long-term study, pumps replaced after 4 weeks; cancer (prostate); peptides; agents given concomitantly in some cases; D-Lys6-LHRH used; 50 U/ml of heparin; animal info (SD, F, 200-250 g).

P2530 Weckbecker,G., Tolcsvai,L., Stolz,B., Pollak,M., Bruns,C. Somatostatin analogue octreotide enhances the antineoplastic effects of tamoxifen and ovariectomy on 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinomas. Cancer Res. 1994; 54(--):6334-6337. >>> Octreotide; Saline; SC; rat; 2002; 6 weeks; controls received mp w/ vehicle; dose-response; long-term study; cancer; peptides.

P2489 Glenthoj,B., Bolwig,T.G., Hemmingsen,R. Effects of chronic discontinuous and continuous treatment of rats with a dopamine D receptor antagonist (NNC-756). Eur.J.Pharmacol. 1993; 242(--):283-291. >>> NNC-756; Acetic acid; Water; SC; rat; 2002; 15 weeks; comparison of Q week injections vs. mp; long-term study, pumps replaced every 2 weeks; dopamine receptor antagonist.

P2468 Pinski,J., Schally,A.V., Yano,T., Szepeshazi,K., Halmos,G., Groot,K., Comaru-Schally,A.M., Rakulovic,S., Nagy,A. Inhibition of growth of experimental prostate cancer in rats by LH-RH analogs linked to cytotoxic radicals. The Prostate 1993; 23(--):165-178. >>> Luteinizing HRH; Methotrexate; AJ-004; G-HMAQ; T-98; Propylene glycol; Water; SC; rat; 2ML4; 7-8 weeks; controls received tumors and mp w/ vehicle; long-term study, pumps replaced after 4 weeks; cancer (prostate); toxicology; peptides; T-98 and AJ-004 are hybrid cytoxic LH-RH analogs; D-Lys6-LHRH used.

P2380 Nylander,A.-G., Chen,D., Lilja,I., Axelson,J., Ihse,I., Rehfeld,J.F., Sundler,F., Hakanson,R. Enterochromaffin-like cells in rat stomach respond to short-term infusion of high doses of cholecystokinin but not to long-term, sustained, moderate hyperCCKemia caused by continuous cholecystokinin infusion or pancreaticobiliary diversion. Scand.J.Gastroenterol. 1993; 28(--):73-79. >>> MK-329; Cholecystokinin 8-sulfate; DMSO; Water; Albumin, bovine serum; Saline; SC; rat; 2002; 7 weeks, 4-7 hours; long-term study, pumps replaced every 10th day; peptides; devazepide is L-364, 718 or MK-329 and is a CCK-A receptor antagonist.

P2302 Bell,S.A., Hobbs,M.V., Rubin,R.L. Isotype-restricted hyperimmunity in a murine model of the toxic oil syndrome. J.Immunol. 1992; 148(--):3369-3376. >>> Oleic acid anilide; Saline; IP; mice; 2002; 6 weeks; Long-term study, pumps replaced every 2 weeks; immunology; toxicology; SC pump connected to IP catheter; fatty acid.

P2297 Sun,Y.-P., Zhu,B.-Q., Sievers,R.E., Glantz,S.A., Parmley,W.W. Metoprolol does not attenuate atherosclerosis in lipid-fed rabbits exposed to environmental tobacco smoke. Circulation 1994; 89(--):2260-2265. >>> Metoprolol; SC; rabbit; 10 weeks; functionality of mp verified by plasma levels of metoprolol; long-term study, pumps replaced every 3 weeks; antihypertensive.

P2277 Geraci,J.P., Taylor,D.A., Mariano,M.S., Jackson,K.L. Effects of dexamethasone on the development of radiation nephropathy in the rat. Radiat.Res. 1992; 131(--):186-191. >>> Dexamethasone; SC; rat; 2ML4; 166 days; comparison of administration in drinking water vs. mp; long-term study, pumps replaced every 4 weeks; benefits of mp admin, p.190.

P2244 de Bruine,A.P., Dinjens,W.N.M., Zijlema,J.H.L., Lenders,M.-H., Bosman,F.T. Renewal of enterochromaffin cells in the rat caecum. Anat.Rec. 1992; 233(--):75-82. >>> Uridine, bromodeoxy-; Ammonia; PBS; SC; rat; 2001; 2002; 1,2,4,8,16,25,31,73 days; long-term study, pumps replaced every 7 days or 14 days.

P2234 Cotter,M.A., Dines,K.C., Cameron,N.E. Prevention and reversal of motor and sensory peripheral nerve conduction abnormalities in streptozotocin-diabetic rats by the prostacyclin analogue iloprost. Naunyn-Schmiedeberg's Arch.Pharmacol. 1993; 347(--):534-540. >>> Iloprost; Water; IV (jugular); rat; 2ML4; 2 months; controls received mp w/vehicle; long-term study, pumps replaced every month; diabetes induced by streptozotocin.

P2209 Zumpe,D., Bonsall,R.W., Michael,R.P. Effects of the nonsteroidal aromatase inhibitor, fadrozole, on the sexual behavior of male cynomolgus monkeys (macaca fascicularis). Horm.Behav. 1993; 27(--):200-215. >>> Fadrozole; Water; SC; monkey; 2ML4; 4,12 weeks; controls received mp/water; long-term study, pumps replaced every 3 weeks; stability verified by plasma levels; pumps implanted in flank.

P2208 Bonsall,R.W., Clancy,A.N., Michael,R.P. Effects of the nonsteroidal aromatase inhibitor, fadrozole, on sexual behavior in male rats. Horm.Behav. 1992; 26(--):240-254. >>> Fadrozole; Water; SC; rat; 2002; 6 weeks; 14 days; controls received mp w/water; long-term study, pumps replaced every 14 days; stability verified by measuring residual solution in pumps; CGS 16949A is fadrozole.

P2175 Bao,G., Gohlke,P., Unger,T. Role of bradykinin in chronic antihypertensive actions of ramipril in different hypertension models. J.Cardiovasc.Pharmacol. 1992; 20(-9-):S96-S99. >>> Hoe 140; Saline; SC; rat; 2002; 6 weeks; long-term study, pumps replaced every 2 weeks; hoe 140 is a bradykinin antagonist; concomitant oral administration of ramipril.

P2174 Bao,G., Gohlke,P., Qadri,F., Unger,T. Chronic kinin receptor blockade attenuates the antihypertensive effect of ramipril. Hypertension 1992; 20(--):74-79. >>> Hoe 140; Saline; SC; rat; 2002; 6 weeks; controls received mp w/saline; long-term study, pumps replaced every 2 weeks; oral ace-inhibitor ramipril administered concomitantly.

P2043 Kibblewhite,D., Larrabee Jr,W.F., Sutton,D. The effect of relaxin on tissue expansion. Arch Otolaryngol.Head Neck Surg. 1992; 118(--):153-156. >>> Relaxin; Saline; SC; pig; 2ML4; 10.5 weeks; long-term study; stress/adverse reaction: one animal extruded osmotic pump during tissue expansion procedure (p. 154); peptides; half-life (p. 155); wound healing.