References on the Administration of Agents Using Multiple ALZET® Osmotic Pumps in a Single Animal

Q0800 Neerhof,M.G., Synowiec,S., Khan,S., Thaete,L.G. Pathophysiology of Chronic Nitric Oxide Synthase Inhibition-Induced Fetal Growth Restriction in the Rat. HYPERTENSION IN PREGNANCY 2011; 30(-1-):28-36. >>> L-NAME; ABT-546; Saline; ethyl alcohol; SC; Rat; 2ML1; 7 days; Controls received mp w/ vehicle; animal info (female, male, Sprague-Dawley, 11-20 wks old, 225-250 g); multiple pumps per animal (2).

Q0641 Suresh,P.S., Jayachandra,K.C., Medhamurthy,R. The effect of progesterone replacement on gene expression in the corpus luteum during induced regression and late luteal phase in the bonnet monkey (Macaca radiata). Reproductive Biology and Endocrinology 2011; 9(-;-):U1-U16. >>> Progesterone; Ethanol; propylene glycol; Monkey; 2ML1; 24 hours; Animal info (adult, female, bonnet, macaca radiata, 3.3-5.1 kg); multiple pumps per animal (3); replacement therapy (corpus leuteum); endocrinology.

Q0932 Thaete,L.G., Neerhof,M.G. ENDOTHELIN AND BLOOD PRESSURE REGULATION IN THE FEMALE RAT: STUDIES INNORMAL PREGNANCY AND WITH NITRIC OXIDE SYNTHASE INHIBITION-INDUCEDHYPERTENSION. HYPERTENSION IN PREGNANCY 2011; 19(-2-):233-247. >>> A-127722; A-182086; A-192621; FR-139317; L-NAME; Ethyl alcohol; propylene glycol; NaOH; water; SC; IV (jugular); Rat (pregnant); rat; 7 days; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley); multiple pumps per animal (2); wound clips used; 20% ethyl alcohol used; "It is likely that administration with the pumps resulted in more consistent drug levels..." pg 242.

Q0675 van Esch,J.H.M., van Veghel,R., Garrelds,I.M., Leijten,F., Bouhuizen,A.M., Danser,A.H.J. Handle Region Peptide Counteracts the Beneficial Effects of the Renin Inhibitor Aliskiren in Spontaneously Hypertensive Rats. Hypertension 2011; 57(-4-):852-858. >>> Handle region peptide; aliskiren; Saline; SC; Rat; 2ML4; 3 weeks; Controls received mp w/ vehicle; animal info (male, SHRs, 280-300g); peptides; multiple pumps per animal (2).

Q0863 Broadbent,N.J., Squire,L.R., Clark,R.E. Sustained Dorsal Hippocampal Activity is Not Obligatory for Either the Maintenance or Retrieval of Long-Term Spatial Memory. Hippocampus 2010; 20(-12-):1366-1375. >>> Lidocaine; CSF/CNS (hippocampus); Rat; 2ML2; 2ML1; 7 days; Controls received mp w/ aCSF; animal info (Long-Evans, naive, male, 300-350 g); good methods "Seven days after minipump implantation, rats were anesthetized with isoflurane gas, the tubing attached to the internal cannula sealed" pg 3; multiple pumps per animal (2); post op. care (Baytril antibiotic); multiple Plastics One cannulae used.

P9936 De Felice,M., Ossipov,M.H., Wang,R.Z., Lai,J., Chichorro,J., Meng,I., Dodick,D.W., Vanderah,T.W., Dussor,G., Porreca,F. Triptan-Induced Latent Sensitization A Possible Basis for Medication Overuse Headache. Annals of Neurology 2010; 67(-3-):325-337. >>> Sumatriptan; naratriptan hydrochloride; GR127935; Saline; SC; Rat; 2001; 6 days; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (adult, male, Sprague Dawley, 175-250 g).

Q0581 De Felice,M., Ossipov,M.H., Wang,R.Z., Dussor,G., Lai,J., Meng,I.D., Chichorro,J., Andrews,J.S., Rakhit,S., Maddaford,S., Dodick,D., Porreca,F. Triptan-induced enhancement of neuronal nitric oxide synthase in trigeminal ganglion dural afferents underlies increased responsiveness to potential migraine triggers. Brain 2010; 133(-;-):2475-2488. >>> Sumatriptan; NXN-323; Saline; SC; Rat; 2001; 6 days; Controls received mp w/ vehicle; animal info (adult, male, Sprague Dawley, 175-250 g); enzyme inhibitor (neuronal nitric oxide synthase); multiple pumps per animal (2).

Q0332 Hajagos-Toth,J., Kormanyos,Z., Falkay,G., Pal,A., Gaspar,R. Potentiation of the uterus-relaxing effects of beta-adrenergic agonists with nifedipine: studies on rats and the human myometrium. Acta Obstetricia et Gynecologica Scandinavica 2010; 89(-10-):1284-1289. >>> Nifedipine; salmeterol xinafoate; PEG 400; ethanol; saline, physiologic; methanol; water; SC; Rat (pregnant); 2ML1; ; Controls received mp w/ vehicle; animal info (Sprague-Dawley, mature, female, 180-200 g, male, 240-260 g); multiple pumps per animal (2).

Q0320 Huang,B.S., Ahmadi,S., Ahmad,M., White,R.A., Leenen,F.H.H. Central neuronal activation and pressor responses induced by circulating ANG II: role of the brain aldosterone-"ouabain" pathway. American Journal of Physiology-Heart and Circulatory Physiology 2010; 299(-2-):U207-U215. >>> Angiotensin II; FAD-286; aldosterone, d-; Saline; CSF, artificial; ethanol; SC; CSF/CNS; Rat; 2001D; 2001; 2002; 2004; 2 weeks; 1, 4 days; Controls received mp w/ vehicle; animal info (male, Wistar, 200-250 g); peptides; dose-response; enzyme inhibitor (aldosterone synthase); multiple pumps per animal (2); telemetry probe (model TA11PA-C40, DSI) used to measure BP and HR; 1% ethanol used with aldosterone.

Q0909 Hutchinson,M.R., Zhang,Y.N., Shridhar,M., Evans,J.H., Buchanan,M.M., Zhao,T.X., Slivka,P.F., Coats,B.D., Rezvani,N., Wieseler,J., Hughes,T.S., Landgraf,K.E., Chan,S., Fong,S., Phipps,S., Falke,J.J., Leinwand,L.A., Maier,S.F., Yin,H., Rice,K.C., Watkins,L.R. Evidence that opioids may have toll-like receptor 4 and MD-2 effects. BRAIN BEHAVIOR AND IMMUNITY 2010; 24(-1-):83-95. >>> Morphine; naloxone; SC; Rat; ; Controls received mp w/ saline; animal info (adult, male, Sprague Dawley, 300-375 g); multiple pumps per animal (2).

Q0020 Kramer,B.W., Kallapur,S.G., Moss,T.J.M., Nitsos,I., Polglase,G.P., Newnham,J.P., Jobe,A.H. Modulation of fetal inflammatory response on exposure to lipopolysaccharide by chorioamnion, lung, or gut in sheep. American Journal of Obstetrics and Gynecology 2010; 202(-1-):U177-U185. >>> Endotoxin, LPS; Saline; Lung (trachea); uterus (amniotic fluid); Sheep (pregnant); 2ML1; 2, 7 days; Controls received mp w/vehicle; teratology; no stress (see pg. 77e2, 77e4); multiple pumps per animal (2); animal info (female, time-mated pregnant).

Q0087 Lucas,G., Du,J., Romeas,T., Mnie-Filali,O., Haddjeri,N., Pineyro,G., Debonnel,G. Selective Serotonin Reuptake Inhibitors Potentiate the Rapid Antidepressant-Like Effects of Serotonin4 Receptor Agonists in the Rat. PLoS One 2010; 5(-2-):U300-U311. >>> Prucalopride; RS 67333; citalopram; SC; Rat; ; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (Sprague Dawley, 250-300 g.).

Q0498 Mazarati,A.M., Pineda,E., Shin,D., Tio,D., Taylor,A.N., Sankar,R. Comorbidity between epilepsy and depression: Role of hippocampal interleukin-1beta. NEUROBIOLOGY OF DISEASE 2010; 37(-2-):461-467. >>> Interleukin-1, receptor antagonist, human, recomb.; Saline, sterile; CSF/CNS (hippocampus); Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit 1 used; multiple pumps per animal (2); animal info (male, Wistar, 50 days old); bilateral hippocampal infusion.

Q0306 Norrby,K., Nordenhem,A. Dalteparin, a low-molecular-weight heparin, promotes angiogenesis mediated by heparin-binding VEGF-A in vivo. APMIS 2010; 118(-12-):949-957. >>> Dalteparin; epirubicin; Saline; SC; Rat; 2002; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (adult, male, Sprague-Dawley, 6-7 wks old, 218-223 g.); cancer; multiple pumps per animal (2); dalteparin is a low-molecular-weight heparin; epirubicin is a chemotherapeutic.

Q0355 Pinnock,S.B., Blake,A.M., Platt,N.J., Herbert,J. The Roles of BDNF, pCREB and Wnt3a in the Latent Period Preceding Activation of Progenitor Cell Mitosis in The Adult Dentate Gyrus by Fluoxetine. PLoS One 2010; 5(-10-):U236-U243. >>> K252a; fluoxetine; Saline; CSF/CNS; SC; Rat; 1002; 2ML2; 7, 14 days; Controls received mp w/ saline; animal info (male, Sprague-Dawley, 8 wks old, 200-250 g); multiple pumps per animal (2); cannula placement verified by examining its track on sections stained with cresyl violet.

Q0132 Sanders,D., Simkiss,D., Braddy,D., Baccus,S., Morton,T., Cannady,R., Weaver,N., Rose,J.E., Levin,E.D. NICOTINIC RECEPTORS IN THE HABENULA: IMPORTANCE FOR MEMORY. Neuroscience 2010; 166(-2-):386-390. >>> Mecamylamine; CSF, artificial; CSF/CNS (lateral habenula nucleus); Rat; 2004; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (female, Sprague Dawley, 3 months old, 200-225 g.); two 28-gauge cannulae used; PE tubing used.

Q0068 Schuhmacher,S., Wenzel,P., Schulz,E., Oelze,M., Mang,C., Kamuf,J., Gori,T., Jansen,T., Knorr,M., Karbach,S., Hortmann,M., Maethner,F., Bhatnagar,A., Foerstermann,U., Li,H., Muenzel,T., Daiber,A. Pentaerythritol Tetranitrate Improves Angiotensin II-Induced Vascular Dysfunction via Induction of Heme Oxygenase-1. Hypertension 2010; 55(-4-):897-U153. >>> Angiotensin II; PETN; ISMN; DMSO; SC; Rat; mice; 7 days; Controls received mp w/ vehicle; multiple pumps per animal; animal info (male, Wistar, 7 wks old, 250 g., HO-1, +/+ or -/-, 3-4 months old); PETN is pentaerythritol tetranitrate; ISMN is isosorbide-5 mononitrate.

Q0066 Shi,P., ez-Freire,C., Jun,J.Y., Qi,Y.F., Katovich,M.J., Li,Q.H., Sriramula,S., Francis,J., Sumners,C., Raizada,M.K. Brain Microglial Cytokines in Neurogenic Hypertension. Hypertension 2010; 56(-2-):297-303. >>> Minocycline; angiotensin II; SC; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ saline; ALZET brain infusion kit used; multiple pumps per animal (2); post op. care (buprenorphine); animal info (male, Sprague Dawley, 6 wks old).

P9972 Trevaskis,J.L., Lei,C.L., Koda,J.E., Weyer,C., Parkes,D.G., Roth,J.D. Interaction of Leptin and Amylin in the Long-term Maintenance of Weight Loss in Diet-induced Obese Rats. Obesity 2010; 18(-1-):21-26. >>> Amylin, rat; leptin, murine; Water, sterile; DMSO; SC; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; peptides; long-term study; multiple pumps per animal (2); animal info (male, DIO, 500 g); pumps replaced after 4 weeks; 50% DMSO for amylin; endocrinology.

Q0190 Troidl,C., Nef,H., Voss,S., Schilp,A., Kostin,S., Troidl,K., Szardien,S., Rolf,A., Schmitz-Rixen,T., Schaper,W., Hamm,C.W., Elsaesser,A., Moellmann,H. Calcium-dependent signalling is essential during collateral growth in the pig hind limb-ischemia model. Journal of Molecular and Cellular Cardiology 2010; 49(-1-):142-151. >>> Phorbol didecanoate, 4-alpha-; IA (femoral); Pig; 2ML1; 7 days; Controls received mp w/ saline or sham operation; cardiovascular; animal info (male, juvenile, crossbred, 38kg, femoral artery occlusion); post op. care (cefquinome, fentanyl); ischemia (hind limb); excellent color photograph of multiple mp placement (Fig. 1); multiple pumps per animal (2).

Q0081 Turek,V.F., Trevaskis,J.L., Levin,B.E., Dunn-Meynell,A.A., Irani,B., Gu,G.B., Wittmer,C., Griffin,P.S., Vu,C., Parkes,D.G., Roth,J.D. Mechanisms of Amylin/Leptin Synergy in Rodent Models. Endocrinology 2010; 151(-1-):143-152. >>> Amylin; leptin; DMSO; water, sterile; SC; Rat; mice; 2004; 2ML4; 28 days; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (lean, male, Sprague Dawley, 426 g., amylin KO, 12 wks old, male, female); one group contained pumps with amylin and leptin; 50% DMSO used; obesity.

Q0601 Zhang,J.F., Villacorta,L., Chang,L., Fan,Z.Z., Hamblin,M., Zhu,T.Q., Chen,C.S., Cole,M.P., Schopfer,F.J., Deng,C.X., Garcia-Barrio,M.T., Feng,Y.H., Freeman,B.A., Chen,Y.E. Nitro-Oleic Acid Inhibits Angiotensin II-Induced Hypertension. Circulation Research 2010; 107(-4-):540-U200. >>> Nitro-oleic acid; oleic acid; angiotensin II; Polyethylene glycol; SC; Mice; 2004; 2002; ; Controls received mp w/ vehicle; animal info (male, C57BL/6J, 8-10 weeks old); peptides; multiple pumps per animal (2); systolic and diastolic BP recordings every 4 h by radiotelemetry.

Q0459 Beaumont,E., Whitaker,C.M., Burke,D.A., Hetman,M., Onifer,S.M. EFFECTS OF ROLIPRAM ON ADULT RAT OLIGODENDROCYTES AND FUNCTIONAL RECOVERY AFTER CONTUSIVE CERVICAL SPINAL CORD INJURY. Neuroscience 2009; 163(-4-):985-990. >>> Rolipram; DMSO; SC; Rat; 2002; ; Spinal cord injury; animal info (female, adult, Sprague Dawley, 228-267 g); multiple pumps per animal (2); stress/adverse reaction: (see pg. 966) "the data of one rat in the rolipram-treated group was removed from the study because of its morbidity".

Q0562 Bennett,S.P., Boyd,T.D., Norden,M., Padmanabhan,J., Neame,P., Wefes,I., Potter,H. A novel technique for simultaneous bilateral brain infusions in a mouse model of neurodegenerative disease. Journal of Neuroscience Methods 2009; 184(-2-):320-326. >>> Antichymotrypsin, alpha-1; CSF, artificial; CSF/CNS; CSF/CNS (hippocampal parenchyma); Mice; 1004; 28 days; Controls received mp w/ vehicle; animal info (PS/APP, PS/APP/ACT, 10 mo old); functionality of mp verified by residual volume; cyanoacrylate adhesive; multiple pumps per animal (2); stability verified ("minimal degradation" after 28 days); custom catheter solution for simultaneous bilateral brain infusions; nitrile cap used to affix custom catheters to the skull; pump plus custom catheter assembly Fig 3e; "entire catheters and pumps we used can be completely removed without terminating the life of the animal" pg 326.

Q0807 Cannady,R., Weir,R., Wee,B., Gotschlich,E., Kolia,N., Lau,E., Brotherton,J., Levin,E.D. Nicotinic antagonist effects in the mediodorsal thalamic nucleus: Regional heterogeneity of nicotinic receptor involvement in cognitive function. Biochemical Pharmacology 2009; 78(-7-):788-794. >>> Nicotine; methyllyaconitine; erythroidine, dihydro-beta-; Saline; CSF, artificial; SC; CSF/CNS (mediodorsal thalamic nucleus); Rat; 2004; 2ML4; 4 weeks; Controls received mp w/ vehicle; animal info (young adult, female, Sprague Dawley); multiple pumps per animal (2); behavioral testing (radial maze); Polypro suturing used to secure the minipumps and tubing; memory.

Q0469 Covington,HE III, Maze,I., LaPlant,Q.C., Vialou,V.F., Ohnishi,Y.N., Berton,O., Fass,D.M., Renthal,W., Rush,AJ III, Wu,E.Y., Ghose,S., Krishnan,V., Russo,S.J., Tamminga,C., Haggarty,S.J., Nestler,E.J. Antidepressant Actions of Histone Deacetylase Inhibitors. Journal of Neuroscience 2009; 29(-37-):11451-11460. >>> MS-275; hydroxamic acid; Cyclodextrin, beta hydroxypropyl; CSF/CNS (nucleus accumbens); Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (9-11 wks old, C57BL/6J, male); enzyme inhibitor (histone deacetylase); multiple pumps per animal (2); Plastics One bilateral cannula used; behavioral testing (social interaction, sucrose preference, forced-swim task, light/dark box); 5% cyclodextrin used; wound clips used; antidepressant.

Q0443 da Silva,A.A., do Carmo,J.M., Freeman,J.N., Tallam,L.S., Hall,J.E. A Functional Melanocortin System May Be Required for Chronic CNS-Mediated Antidiabetic and Cardiovascular Actions of Leptin. Diabetes 2009; 58(-8-):1749-1756. >>> Leptin; SHU-9119; CSF/CNS; Rat; 2002; 10, 17 days; Animal info (male, Sprague-Dawley, 325-350 g); pumps replaced after 7 days; multiple pumps per animal (2); guide cannula used; cannula placement verified by angiotensin II dipsogenic effect; diabetes; Leptin and SHU-9119 attached to Y connector.

P9406 Ferreira,A.J., Shenoy,V., Yamazato,Y., Sriramula,S., Francis,J., Yuan,L., Castellano,R.K., Ostrov,D.A., Oh,S.P., Katovich,M.J., Raizada,M.K. Evidence for Angiotensin-converting Enzyme 2 as a Therapeutic Target for the Prevention of Pulmonary Hypertension. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 2009; 179(-11-):1048-1054. >>> XNT; A-779; Saline; SC; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; cardiovascular; antihypertensive; multiple pumps per animal (2); animal info (male, Sprague Dawley, 12 wks old); A-779 is a Mas (Ang (1-7) receptor) antagonist; XNT is an ACE2 synthetic activator; saline was low pH.

Q0603 Gallego,J.M., Ortiz,L., Gutierrez,R., Barcia,J.A. Continuous bilateral infusion of GABA in the dorsomedian nucleus of the thalamus elevates the generalized seizure threshold in amygdala-kindled rats. SEIZURE-EUROPEAN JOURNAL OF EPILEPSY 2009; 18(-7-):537-540. >>> Aminobutyric acid, gamma; Saline; CSF/CNS (dorsomedian nuclei of the thalamus); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (adult, male, Wistar); functionality of mp verified by residual volume; multiple pumps per animal (2); bilateral infusion.

P9788 Ginovart,N., Wilson,A.A., Hussey,D., Houle,S., Kapur,S. D2-Receptor Upregulation is Dependent upon Temporal Course of D2-Occupancy: A Longitudinal [11C]-Raclopride PET Study in Cats. Neuropsychopharmacology 2009; 34(-3-):662-671. >>> Haloperidol; SC; Cat; 2ML4; 4 weeks; Comparison of bolus injections vs. SC mp; multiple pumps per animal (4).

Q0455 Gotfredsen,K., Lindh,C.H., Berglundh,T. Does Longstanding Nicotine Exposure Impair Bone Healing and Osseointegration? An Experimental Study in Rabbits. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2009; 91B(-2-):918-923. >>> Nicotine tartrate; Water, sterile; SC; Rabbit; 6 months; Animal info (New Zealand, white, female); long-term study; pumps replaced monthly; multiple pumps per animal (2); "the results indicate the relative safety of nicotine replacement therapy for periods up to 6 months." pg 923.

Q0543 Hutchinson,M.R., Lewis,S.S., Coats,B.D., Skyba,D.A., Crysdale,N.Y., Berkelhammer,D.L., Brzeski,A., Northcutt,A., Vietz,C.M., Judd,C.M., Maier,S.F., Watkins,L.R., Johnson,K.W. Reduction of opioid withdrawal and potentiation of acute opioid analgesia by systemic AV411 (ibudilast). BRAIN BEHAVIOR AND IMMUNITY 2009; 23(-2-):240-250. >>> Morphine; oxycodone; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (pathogen-free, male, Sprague-Dawley, 300-350 g); multiple pumps per animal (2); PE60 catheter connected to pump for 2-day delayed low-dose infusion.

P9640 Kang,Y.M., Ma,Y., Zheng,J.P., Elks,C., Sriramula,S., Yang,Z.M., Francis,J. Brain nuclear factor-kappa B activation contributes to neurohumoral excitation in angiotensin II-induced hypertension. Cardiovascular Research 2009; 82(-3-):503-512. >>> Angiotensin II; losartan; pyrrolidine dithiocarbamate; tempol; Saline; CSF, artificial; IV (femoral); CSF/CNS; Rat; 4 weeks; Controls received mp w/vehicle; peptides; multiple pumps per animal (2); animal info (adult, male, Sprague-Dawley, 275-300g); bilateral cannula used for multiple drug infusions.

Q0613 Kawasaki,M., Ponzio,T.A., Yue,C.M., Fields,R.L., Gainer,H. Neurotransmitter regulation of c-fos and vasopressin gene expression in the rat supraoptic nucleus. Experimental Neurology 2009; 219(-1-):212-222. >>> TTX; CNQX; APV; Saline; CSF/CNS (supraoptic nucleus); Rat; 2001D; 3.5 hours; Controls received mp w/ vehicle; animal info (adult, male, Spraque-Dawley, 250-350 g); functionality of mp verified by measuring Fluorogold in the brain; multiple pumps per animal (2); multiple cannulae (2, 28 gauge) used; pumps not implanted but activated by immersion in 0.9% saline at 37°C.; PE-60 tubing used.

P9421 Kobayashi,N., Takeshima,H., Fukushima,H., Koguchi,W., Mamada,Y., Hirata,H., Machida,Y., Shinoda,M., Suzuki,N., Yokotsuka,F., Tabei,K., Matsuoka,H. Cardioprotective Effects of Pitavastatin on Cardiac Performance and Remodeling in Failing Rat Hearts. American Journal of Hypertension 2009; 22(-2-):176-182. >>> Pitavastatin; Wortmannin; Y-27632; DMSO; saline; SC; Rat; 2ML4; 7 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced; enzyme inhibitor (phosphatidyl inositol 3-kinase, Rho kinase); cardiovascular; multiple pumps per animal (2); animal info (male, Dahl S, Dahl R, 11 wks old); 8% DMSO used.

P9649 Levin,E.D., Perkins,A., Brotherton,T., Qazi,M., Berez,C., Montalvo-Ortiz,J., Davis,K., Williams,P., Christopher,N.C. Chronic underactivity of medial frontal cortical beta2-containing nicotinic receptors increases clozapine-induced working memory impairment in female rats. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY&BIOLOGICAL PSYCHIATRY 2009; 33(-2-):296-302. >>> Nicotine; methllycaconitine; erithrodine, dihydro-beta; Saline; CSF, artificial; SC; CSF/CNS (medial frontal cortex); Rat; 2004; 2ML4; 4 weeks; Controls received mp w/vehicle; animal info (adult female, SD); multiple pumps per animal (2); bilateral infusion; behavioral testing (radial-Arm-maze).

Q0435 Muhlhausler,B.S., Morrison,J.L., McMillen,I.C. Rosiglitazone Increases the Expression of Peroxisome Proliferator-Activated Receptor-gamma Target Genes in Adipose Tissue, Liver, and Skeletal Muscle in the Sheep Fetus in Late Gestation. Endocrinology 2009; 150(-9-):4287-4294. >>> Rosiglitazone; Ethanol; water; SC; Sheep (ewe); 2ML4; ; Controls received mp w/ vehicle; animal info (adult, Merino, 123-126 days old); multiple pumps per animal (4); 7% ethanol used; endocrinology.

Q0529 Mukaddam-Daher,S., Menaouar,A., Paquette,P.A., Jankowski,M., Gutkowska,J., Gillis,M.A., Shi,Y.F., Calderone,A., Tardif,J.C. Hemodynamic and Cardiac Effects of Chronic Eprosartan and Moxonidine Therapy in Stroke-Prone Spontaneously Hypertensive Rats. Hypertension 2009; 53(-5-):775-U58. >>> Moxonidine; eprosartan; Saline; NaOH; HCl; SC; Rat; 2004; 2ML4; 8 weeks; Controls received mp w/ vehicle; animal info (SP-SHR, 14 wks old, 250-275 g); antihypertensive; pumps replaced after 4 weeks; long-term study; multiple pumps per animal (2); moxonidine or eprosartan were infused separately or in combination.

Q0364 Oslund,K.L., Hyde,D.M., Putney,L.F., Alfaro,M.F., Walby,W.F., Tyler,N.K., Schelegle,E.S. Activation of Calcitonin Gene-Related Peptide Receptor during Ozone Inhalation Contributes to Airway Epithelial Injury and Repair. Toxicologic Pathology 2009; 37(-6-):805-813. >>> Uridine, bromodeoxy; C-2806 8-37; NaOH; IV (jugular); SC; Rat; 2001D; ; Controls received mp w/ sterile saline; animal info (male, Wistar, 200-250 g); peptide; multiple pumps per animal (2); C-2806 8-37 is a CGRP receptor antagonist; wound clips used; labeling of airway epithelium.

Q0286 Rosi,S., Ramirez-Amaya,V., Vazdarjanova,A., Esparza,E.E., Larkin,P.B., Fike,J.R., Wenk,G.L., Barnes,C.A. Accuracy of hippocampal network activity is disrupted by neuroinflammation: rescue by memantine. Brain 2009; 132(-;-):2464-2477. >>> Endotoxin, LPS; memantine; CSF/CNS (fourth ventricle); SC; Rat; 2004; 2ML4; 4 weeks; Controls received mp w/ aCSF; animal info (3-month-old, male, F-344, Sprague Dawley); multiple pumps per animal (2); no stress (see pg 2468) "No adverse neurological signs were observed..."; behavioral testing (exploration).

P9086 Thomale,U., Tyler,B., Renard,V., Dorfman,B., Guarnieri,M., Haberl,H., Jallo,G. Local chemotherapy in the rat brainstem with multiple catheters: a feasibility study. Childs Nervous System 2009; 25(-1-):21-28. >>> Carboplatin; Dextrose; CSF/CNS (parenchyma); Rat; 2001; 7 days; Controls received mp w/ vehicle; multiple pumps per animal (2, 3); animal info (male, Fisher); polyethylene tubing (PE) used; x-ray of skull showing cannulae position; pg. 22 illustration of pump and implanted cannulae.

P9792 Uekita,T., Okaichi,H. Pretraining Does Not Ameliorate Spatial Learning Deficits Induced by Intrahippocampal Infusion of AP5. Behavioral Neuroscience 2009; 123(-3-):520-526. >>> AP5; CSF, artificial; CSF/CNS (hippocampus); Rat; 1002; 14 days; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (male, albino, Wistar, 2 months old); Plastics One cannula used; silicon rubber used.

Q0517 Wang,X., Chow,F.L., Oka,T., Hao,L., Lopez-Campistrous,A., Kelly,S., Cooper,S., Odenbach,J., Finegan,B.A., Schulz,R., Kassiri,Z., Lopaschuk,G.D., Fernandez-Patron,C. Matrix Metalloproteinase-7 and ADAM-12 (a Disintegrin and Metalloproteinase-12) Define a Signaling Axis in Agonist-Induced Hypertension and Cardiac Hypertrophy. Circulation 2009; 119(-18-):2480-U112. >>> Oligonucleotide, antisense; oligonucleotide, scrambled; RNA, small interferring; angiotensin II; PBS; SC; Mice; rat; 14 days; Controls received mp w/ vehicle; animal info (MMP-7-/-, wt, 12 wks old; SHR, WKY, Sprague Dawley, 250-350 g); peptides; antisense (MMP-7); multiple pumps per animal (2); blood pressure measured indirectly using a computerized tail cuff plethysmography system.

P9459 Wei,S.G., Yu,Y., Zhang,Z.H., Felder,R.B. Angiotensin II upregulates hypothalamic AT1 receptor expression in rats via the mitogen-activated protein kinase pathway. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1425-H1433. >>> Angiotensin II; losartan; PD-98059; SP-600125; SB-203580; Saline; DMSO; CSF, artificial; SC; CSF/CNS; Rat; 2002; 2004; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; enzyme inhibitor (MAP kinase, Jun N-terminal kinase); cardiovascular; peptides; multiple pumps per animal (2); animal info (male, Sprague Dawley, 275-300 g.); cannula placement confirmed at end of study; 0.1%-0.5% DMSO used.

P9880 Wu,K.L.H., Hsu,C., Chan,J.Y.H. Nitric Oxide and Superoxide Anion Differentially Activate Poly(ADP-ribose) Polymerase-1 and Bax to Induce Nuclear Translocation of Apoptosis-Inducing Factor and Mitochondrial Release of Cytochrome c after Spinal Cord Injury. Journal of Neurotrauma 2009; 26(-7-):965-977. >>> Methylisothiourea, S-; PTIO, carboxy-; Coenzyme Q10; tempol; FeTMPyP; furosemide; PJ-34; CSF, artificial; sesame oil; CSF/CNS (spinal cord); Rat; 1003D; 3 days; Controls received mp w/ vehicle; multiple pumps per animal (2); post op. care (procraine penicillin); adult, male, specific pathogen free, 200-250 g.); spinal cord injury; PE-10 catheter used; PTIO is a NO trapping agent; enzyme inhibitor PJ-34 is poly(ADP-ribose) polymerase PARP inhibitor.

P9466 Xing,J.H., Lu,J., Li,J.H. Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1380-H1387. >>> Nerve growth factor; SC; Rat; 1003D; 72 hours; Controls received mp w/ saline to contralateral leg; cardiovascular; peptides; multiple pumps per animal (2); ischemia (muscle); animal info (male, Sprague Dawley, 5-7 wks old); SC in the hindlimb.

Q0568 Xu,J., Kirigiti,M.A., Grove,K.L., Smith,M.S. Regulation of Food Intake and Gonadotropin-Releasing Hormone/Luteinizing Hormone during Lactation: Role of Insulin and Leptin. Endocrinology 2009; 150(-9-):4231-4240. >>> Insulin, human; leptin, rat; Saline; SC; Rat; 1003D; 48 hours; Controls received mp w/ vehicle; animal info (d 8-9 post partum, 6 d post ovariectomy); multiple pumps per animal (2); replacement therapy (ovariectomized).

P9467 Xue,B.J., Badaue-Passos,D.Jr, Guo,F., Gomez-Sanchez,C.E., Hay,M., Johnson,A.K. Sex differences and central protective effect of 17 beta-estradiol in the development of aldosterone/NaCl-induced hypertension. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1577-H1585. >>> Aldosterone; estradiol, 17b-; ICI 182,780; SC; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy, castration); cardiovascular; multiple pumps per animal (2); animal info (male, Sprague Dawley, 10-12 wks old); cannula placement verified histologically; estrogen receptor antagonist; endocrinology.

P9503 Alvarez-Fischer,D., Guerreiro,S., Hunot,S., Saurini,F., Marien,M., Sokoloff,P., Hirsh,E.C., Hartmann,A., Michel,P.P. Modelling Parkinson-like neurodegeneration via osmotic minipump delivery of MPTP and probenecid. Journal of Neurochemistry 2008; 107(-3-):701-711. >>> MPTP HCl; probenecid; Saline; SC; IP; Mice; 1002; 2004; 2, 3, 4 weeks; 5 days; Controls received mp w/ vehicle; dose-response (fig 3); comparison of IP injections vs. mp; multiple pumps per animal (2); animal info (male, C57BL/6, 9-10 wks old); neurodegenerative (Parkinson's disease); probenecid used as an adjuvant molecule, serving as a solvent for MPTP in some experiments.

P9078 Billes,S.K., Cowley,M.A. Catecholamine reuptake inhibition causes weight loss by increasing locomotor activity and thermogenesis. Neuropsychopharmacology 2008; 33(-6-):1287-1297. >>> Nisoxetine; GBR12783; bupropion; DMSO; saline, nonpyrogenic; SC; Mice; 1007D; 2001; 7 days; Controls received mp w/vehicle; multiple pumps per animal (2); animal info (adult, male, C57BL/6J); 10% DMSO used; "we were limited to 7 days by drug solubility." pg. 1288; incision was closed with wound clips; nisoxetine is a norepinephrine reuptake inhibitor; GBR12783 is a dopamine reuptake inhibitor; "This study emphasizes that although acute studies may infer long-term drug effects, they do not necessarily predict a drug's chronic effects. Even though the chronic studies presented here were limited (by method of administration and drug solubility) to 1 week of treatment, they were sufficient to illustrate differences between the acute and subchronic effects of catecholamine reuptake inhibitors.".

P9392 Bosch,O.J., Neumann,I.D. Brain vasopressin is an important regulator of maternal behavior independent of dams' trait anxiety. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-44-):17139-17144. >>> Vasopressin, arginine; oxytocin; oligonucleotide, antisense; oligonucleotide, missense; Ringer's solution; CSF/CNS; CSF/CNS (medial preoptic area); Rat (pregnant); 1007D; 5-7 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; antisense (AVP-R); peptides; multiple pumps per animal (2); post op. care (Enrofloxacin, Baytril); animal info (female, Wistar, HAB, LAB, 14 wks old); behavioral testing (maternal care, pup retrieval test, elevated plus maze); cannula placement and infusion sites verified with blue ink.

R0256 Bunge,M.B. Novel combination strategies to repair the injured mammalian spinal cord. JOURNAL OF SPINAL CORD MEDICINE 2008; 31(-3-):262-269. >>> Rolipram; SC; Rat; 1007D; 2 weeks; Pumps replaced after 1 week; enzyme inhibitor (phosphpodiesterase-4); multiple pumps per animal (2); animal info (female, Fischer, SCI); review, see pgs. 266-267; behavioral testing (locomotion).

P9382 Feldmann,G., Habbe,N., Dhara,S., Bisht,S., Alvarez,H., Fendrich,V., Beaty,R., Mullendore,M., Karikari,C., Bardeesy,N., Ouellette,M.M., Yu,W., Maitra,A. Hedgehog inhibition prolongs survival in a genetically engineered mouse model of pancreatic cancer. Gut 2008; 57(-10-):1420-1430. >>> Cyclopamine; Sodium citrate buffer; phosphate buffer; cyclodextrin, 2-hydroxy-propyl-beta-; SC; Mice (transgenic); 5, 61-67 days; Controls received mp w/ vehicle; long-term study; pumps replaced weekly; cancer (panreatic adenocarcinoma); multiple pumps per animal (2); animal info (Pdx1-Cre; LsL-Kras-G12D; Ink4a/ArF-lox/lox Tg, 5 wks old); "pharmacological Hedgehog blockage by continuous administration of low-dose cyclopamine prolongs survival in this model (of pancreatic cancer)." (p. 1420); 30% cyclodextrin used.

P9285 Feldmann,G., Fendrich,V., McGovern,K., Bedja,D., Bisht,S., Alvarez,H., Koorstra,J.B.M., Habbe,N., Karikari,C., Mullendore,M., Gabrielson,K.L., Sharma,R., Matsui,W., Maitra,A. An orally bioavailable small-molecule inhibitor of Hedgehog signaling inhibits tumor initiation and metastasis in pancreatic cancer. MOLECULAR CANCER THERAPEUTICS 2008; 7(-9-):2725-2735. >>> IPI-269609; Cyclodextrin, 2-hydroxypropyl-b-; water, sterile; SC; Mice (nude); 2002; 6 weeks; 5, 7 days; Controls received mp w/ vehicle; comparison of daily PO vs. mp; pumps replaced every 2 wks; no stress (see pg. 2734); cancer (pancreatic); multiple pumps per animal (2); animal info (male, athymic CD1 nu/nu, 6-12 wks old); 30% cyclodextrin; Hedgehog inhibitor; "at the applied doses there were no signs of toxicity, weight loss, or other signs of adverse events in mice during the described treatment periods." (p. 2734).

P9230 Hashimoto,M., Ishii,K., Nakamura,Y., Watabe,K., Kohsaka,S., Akazawa,C. Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury. Journal of Neurochemistry 2008; 107(-4-):918-927. >>> Cyclopamine; Cyclodextrin, 2-hydroxypropyl-b-; CSF/CNS (sciatic nerve); Rat; 2001; 1 week; Controls received mp w/ vehicle to contralateral side; multiple pumps per animal (2); animal info (male, Wistar, 7 wks old, 200-250 g., sciatic nerve injury); 45% cyclodextrin used.

P9650 Hayashi,S., Fujii,E., Kato,A., Kimura,K., Mizoguchi,K., Suzuki,M., Sugimoto,T., Takanashi,H., Itoh,Z., Omura,S., Wanibuchi,H. Characterization of Multinuclear Hepatocytes Induced in Rats by Mitemcinal (GM-611), an Erythromycin Derivative. Toxicologic Pathology 2008; 36(-6-):858-865. >>> Uridine, bromodeoxy-; DMSO; water, distilled; IP; Rat; 2002; 28 days; Pumps replaced after 14 days; animal info (Sprague Dawley, female); multiple pumps per animal (2); 50% DMSO used.

P8813 Kubota,Y., Takubo,K., Suda,T. Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche. Biochemical and Biophysical Research Communications 2008; 366(-2-):335-339. >>> Uridine, bromodeoxy-; PBS; SC; Mice; 2004; 2 months; Long-term study; pumps replaced after 28 days; multiple pumps per animal (2); animal info (C57BL/6, 1 month old); labeling of bone marrow LRC's.

P9279 Mueller,L.U.W., Schore,R.J., Zheng,Y., Thomas,E.K., Kim,M.O., Cancelas,J.A., Gu,Y., Williams,D.A. Rac guanosine triphosphatases represent a potential target in AML. LEUKEMIA 2008; 22(-9-):1803-1806. >>> NSC23766; Mice (SCID); 28 days; Controls received mp w/ PBS; pumps replaced after 14 days; enzyme inhibitor (Rac GTPas); cancer (acute myeloid leukemia); multiple pumps per animal (2); animal info (NOD/SCID, irradiated).

P9795 Phongsisay,V., Susuki,K., Matsuno,K., Yamahashi,T., Okamoto,S., Funakoshi,K., Hirata,K., Shinoda,M., Yuki,N. Complement inhibitor prevents disruption of sodium channel clusters in a rabbit model of Guillain-Barre syndrome. Journal of Neuroimmunology 2008; 205(-1-2-):101-104. >>> Nafamostat mesilate; DMSO; IV (jugular); Rabbit; 2ML1; 7 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; enzyme inhibitor (serine protease); multiple pumps per animal (2); animal info (White); 50% DMSO used.

P9252 Qin,Z.Y., Gongora,M.C., Ozumi,K., Itoh,S., Akram,K., Ushio-Fukai,M., Harrison,D.G., Fukai,T. Role of Menkes ATPase in Angiotensin II-Induced Hypertension A Key Modulator for Extracellular Superoxide Dismutase Function. Hypertension 2008; 52(-5-):945-U556. >>> Angiotensin II; norepinephrine; tempol; Saline; SC; Mice; 2002; 14 days; Controls received sham surgery with no mp or empty mp; functionality of mp verified by blood pressure; cardiovascular; peptides; multiple pumps per animal (2); animal info (female, C57BL/6EJ blotchy MNK mut, 8-12 wks old).

P9105 Roth,J.D., Trevaskis,J.L., Wilson,J., Lei,C., Athanacio,J., Mack,C., Kesty,N.C., Coffey,T., Weyer,C., Parkes,D.G. Antiobesity effects of the beta-cell hormone amylin in combination with phentermine or sibutramine in diet-induced obese rats. Int.J.Obes.(Lond) 2008/8; 32(-8-):1201-1210. >>> Amylin, rat; sibutramine; phentermine; DMSO; water; SC; Rat; 2 weeks; Controls received mp w/ vehicle; dose-response (table 2); multiple pumps per animal (2); animal info (male, DIO-prone, 450-500 g.); Research Diets D12266B; obesity; 50% DMSO used.

P9441 Sakai,A., Asada,M., Seno,N., Suzuki,H. Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain. Pain 2008; 137(-2-):378-388. >>> Oligodeoxynucleotide, phosphorothioate antisense; glial-derived neurotrophic factor, recomb. human; neural cell adhesion molecule, mimetic peptide C3d; oligodeoxynucleotide, phosphorothioate missense; CSF/CNS (subarachnoid space); Rat; 4, 6, 7, 9 days; Controls received mp w/ saline; antisense (NCAM); peptides; multiple pumps per animal (2); animal info (male, Sprague Dawley, 5 wks old, 140-160 g., sciatic nerve injury); behavioral testing (mechanical allodynia, thermal hypersensitivity, paw withdrawal, plantar test).

P9144 Teitelbaum,A.A., Gareau,M.G., Jury,J., Yang,P.C., Perdue,M.H. Chronic peripheral administration of corticotropin-releasing factor causes colonic barrier dysfunction similar to psychological stress. American Journal of Physiology-Gastrointestinal and Liver Physiology 2008; 295(-3-):G452-G459. >>> Corticotropin-rel factor; Stressin1; sauvagine, anti-; urocortin III; Saline; SC; Rat; 2002; 12 days; Controls received mp w/ vehicle; peptides; multiple pumps per animal (2); animal info (mast cell-deficient, +/+, 10 wks old, 200-250 g.); "The use of the minipump avoids daily interactions with the animals, possibly causing less variability in the results." pg G458; stressin1 is a selective CFR-R1 agonist; urocortin III is a CFR-R2 agonist; antisauvagine is a CRF-R2 antagonist.

P9454 Thomsen,M., Fink-Jensen,A., Woldbye,D.P.D., Woertwein,G., Sager,T.N., Holm,R., Pepe,L.M., Caine,S.B. Effects of acute and chronic aripiprazole treatment on choice between cocaine self-administration and food under a concurrent schedule of reinforcement in rats. Psychopharmacology 2008; 201(-1-):43-53. >>> Aripiprazole; Cyclodextrin, hydroxypropyl-b-; methylsulfonic acid; NaOH; SC; Rat; 2ML1; 5 days; Controls received mp w/ lowest dose which had no effect; dose-response (fig. 2); half-life (p. 46) 2 hours in rats, 60 hours in humans; tolerance; dependence; multiple pumps per animal (2); animal info (male, Sprague Dawley, 9 wks old, 400-500 g.); behavioral testing (cocaine self-administration); 20% cyclodextrin used.

P9173 Trevaskis,J.L., Coffey,T., Cole,R., Lei,C., Wittmer,C., Walsh,B., Weyer,C., Koda,J., Baron,A.D., Parkes,D.G., Roth,J.D. Amylin-Mediated Restoration of Leptin Responsiveness in Diet-Induced Obesity: Magnitude and Mechanisms. Endocrinology 2008; 149(-11-):5679-5687. >>> Leptin, murine; amylin, rat; Water, sterile; DMSO; Rat; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (DIO-prone, Sprague Dawley); 50% DMSO used; Research Diets D1226B.

P9135 Wenzel,P., Mollnau,H., Oelze,M., Schulz,E., Wickramanayake,J.M.D., Mueller,J., Schuhmacher,S., Hortmann,M., Baldus,S., Gori,T., Brandes,R.P., Muenzel,T., Daiber,A. First evidence for a crosstalk between mitochondrial and NADPH oxidase-derived reactive oxygen species in nitroglycerin-triggered vascular dysfunction. ANTIOXIDANTS&REDOX SIGNALING 2008; 10(-8-):1435-1447. >>> Nitroglycerin; apocynin; glibenclamide; Ethanol; SC; Rat; 1003D; 1007D; 3, 7 days; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (male, Wistar, 250 g.).

P9461 Whitaker,C.M., Beaumont,E., Wells,M.J., Magnuson,D.S.K., Hetman,M., Onifer,S.M. Rolipram attenuates acute oligodendrocyte death in the adult rat ventrolateral funiculus following contusive cervical spinal cord injury. Neuroscience Letters 2008; 438(-2-):200-204. >>> Rolipram; DMSO; SC; Rat; 2002; 12, 24, 72 hours; Controls received mp w/ vehicle; enzyme inhibitor (phosphodiesterase 4); multiple pumps per animal (2); post op. care (Gentozen, bacitracin zinc); animal info (female, Sprague Dawley, 228-267 g., SCI).

P8371 Billes,S.K., Cowley,M.A. Inhibition of dopamine and norepinephrine reuptake produces additive effects on energy balance in lean and obese mice. Neuropsychopharmacology 2007; 32(-4-):822-834. >>> Nisoxetine; GBR12783; Saline, sterile nonpyrogenic; water sterile; DMSO; SC; Mice; 1007D; 2001; 1 week; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (male, C57BL/6J); 10% DMSO (NIS); 50% DMSO (GBR).

P8600 de Seigneux,S., Nielsen,J., Olesen,E.T.B., Dimke,H., Kwon,T.H., Frokiaer,J., Nielsen,S. Long-term aldosterone treatment induces decreased apical but increased basolateral expression of AQP2 in CCD of rat kidney. American Journal of Physiology- Renal Physiology 2007; 293(-1-):F87-F99. >>> Aldosterone; vasopressin, 1-deamino-8-D-arginine; angiotensin II; Saline; DMSO; ringer's solution, lactated; SC; Rat; 2001; 2002; 6,7,8 days; Controls received mp w/ vehicle; cardiovascular; peptides; multiple pumps per animal (2); post op. care (Buprenorphine); animal info (male, Wistar-Hanover, 220-250 grams, male, Sprague-Dawley, 190-210 grams, male, Munich-Wistar, 220 grams); 30% and 25% DMSO used.

P8569 Dedkov,E.I., Zheng,W., Christensen,L.P., Weiss,R.M., Mahlberg-Gaudin,F., Tomanek,R.J. Preservation of coronary reserve by ivabradine-induced reduction in heart rate in infarcted rats is associated with decrease in perivascular collagen. Am.J.Physiol Heart Circ.Physiol 2007/7; 293(-1-):H590-H598. >>> Ivabradine; bromodeoxy; Dextrose; IP; Rat; 4 weeks; 2,6,13 days; 72 hours; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (male, Sprague-Dawley, 12 months old, MI); ischemia (cardiac).

P8923 Deseure,K., Breand,S., Colpaert,F.C. Curative-like analgesia in a neuropathic pain model: Parametric analysis of the dose and the duration of treatment with a high-efficacy 5-HT1A receptor agonist. European Journal of Pharmacology 2007; 568(-1-3-):134-141. >>> F13640; Acetate buffer; water, sterile; SC; Rat; 2ML1; 2ML2; 2ML4; 2, 4, 8 weeks; 1, 2, 4, 7 days; Controls received mp w/ vehicle; dose-response (fig. 2); long-term study; pumps replaced; half-life (p. 139) 2.5 hrs. in rat plasma; multiple pumps per animal (2); post op. care (Na ampicillin); animal info (male, Sprague Dawley, 220-240 g., intraorbital nerve ligation).

P8257 Elmarakby,A.A., Williams,J.M., Imig,J.D., Pollock,J.S., Pollock,D.M. Synergistic actions of enalapril and ternpol during chronic angiotensin II-induced hypertension. VASCULAR PHARMACOLOGY 2007; 46(-2-):144-151. >>> Angiotensin II; icatibant; SC; Rat; 2002; 14 days; Controls received no treatment; functionality of mp verified by systolic blood pressure; cardiovascular; peptides; multiple pumps per per animal (2); animal info (male, Sprague-Dawley, 200-250g.).

P8201 Grobe,J.L., Mecca,A.P., Lingis,M., Shenoy,V., Bolton,T.A., Machado,J.M., Speth,R.C., Raizada,M.K., Katovich,M.J. Prevention of angiotensin II-induced cardiac remodeling by angiotensin-(1-7). American Journal of Physiology-Heart and Circulatory Physiology 2007; 292(-2-):H736-H742. >>> Angiotensin II; angiotensin (1-7); angiotensin (1-7), D-ALa7-; SC; Rat; 2004; 4 weeks; Controls received sham treatment; cardiovascular; peptides; multiple pumps per animal (2); animal info (male, Sprague-Dawley, 270-300g.).

P8536 Joyce,M.M., Burghardt,J.R., Burghardt,R.C., Hooper,R.N., Jaeger,L.A., Spencer,T.E., Bazer,F.W., Johnson,G.A. Pig conceptuses increase uterine interferon-regulatory factor 1 (IRF1), but restrict expression to stroma through estrogen- induced IRF2 in luminal epithelium. Biology of Reproduction 2007; 77(-2-):292-302. >>> Conceptus secretory proteins, porcine; Albumin, porcine serum; dye, india ink; PBS; Intrauterine (uterine horn); Pig; 2ML1; 4 days; Controls received mp w/ PSA; peptides; multiple pumps per animal (2); animal info (female, pseudopregnant); india ink used to confirm agent distribution in uterus; tissue perfusion (uterine horn).

P8680 Joyce,M.M., Burghardt,R.C., Geisert,R.D., Burghardt,J.R., Hooper,R.N., Ross,J.W., Ashworth,M.D., Johnson,G.A. Pig conceptuses secrete estrogen and interferons to differentially regulate uterine STAT1 in a temporal and cell type-specific manner. Endocrinology 2007; 148(-9-):4420-4431. >>> Conceptus secretory proteins, porcine; Intrauterine; Pig; 2ML1; ; Controls received mp w/ albumin, porcine serum; tissue perfusion (uterus); good methods p. 4421; peptides; multiple pumps per animal (2); animal info (female).

P8677 King,T., Vardanyan,A., Majuta,L., Melemedjian,O., Nagle,R., Cress,A.E., Vanderah,T.W., Lai,J., Porreca,F. Morphine treatment accelerates sarcoma-induced bone pain, bone loss, and spontaneous fracture in a murine model of bone cancer. Pain 2007; 132(-1-2-):154-168. >>> Morphine; naloxone; Saline; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; dose-response (p. 157, 159); no stress (see pg. 155); cancer (bone); multiple pumps per animal (2); animal info (20-25g, male, adult, C3H/HeJ); pain.

P8705 Mizuno,M., Sotoyama,H., Narita,E., Kawamura,H., Namba,H., Zheng,Y.J., Eda,T., Nawa,H. A cyclooxygenase-2 inhibitor ameliorates behavioral impairments induced by striatal administration of epidermal growth factor. Journal of Neuroscience 2007; 27(-38-):10116-10127. >>> Epidermal growth factor, recomb. human; Saline; CSF/CNS (striatum); CSF/CNS (nucleus accumbens); Rat; 2002; 8-14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (fig. 1); peptides; multiple pumps per animal (2); post op. care (cefmetazon); animal info (male, Sprague-Dawley, 7-8 wks old, 300-380g); cannula position confirmed after completion of tests.

P8534 Ni,W., Zhan,Y., He,H., Maynard,E., Balschi,J.A., Oettgen,P. Ets-1 is a critical transcriptional regulator of reactive oxygen species and p47phox gene expression in response to angiotensin II. Circulation Research 2007; 101(-10-):985-994. >>> Angiotensin II; Ets-1, dominant negative peptide; peptide, Ets-1, mutated; Saline; SC; Mice; 2 weeks; Controls received mp w/ vehicle or mutant control peptide; cardiovascular; peptides; multiple pumps per animal (2); animal info (C57BL/6, wt or Ets-1 -/-); peptide presence in thoracic aorta confirmed by immunoflorescence; DN-Ets-1 peptide biotin linked and HIV-TAT (48-60) linked.

P8399 Perry,T., Holloway,H.W., Weerasuriya,A., Mouton,P.R., Duffy,K., Mattison,J.A., Greig,N.H. Evidence of GLP-1-mediated neuroprotection in an animal model of pyridoxine-induced peripheral sensory neuropathy. Experimental Neurology 2007; 203(-2-):293-301. >>> Glucagon-like peptide-1 (7-36)-amide; exendin-4; exendin (9-39); peptide, inactive; SC; Rat; 14 days; Controls received mp w/ scrambled, inactive, 30aa peptide; functionality of mp verified by plasma GLP-1 levels; dose-response (fig. 3); half-life (pg. 293) 1.5 min. in rodents (GLP-1), 120 min. in rodents (Ex4); peptides; multiple pumps per animal (2); animal info (male, Sprague-Dawley, 300-350g.).

P8972 Prabhakar,S., Starnes,J., Shi,S., Lonis,B., Tran,R. Diabetic nephropathy is associated with oxidative stress and decreased renal nitric oxide production. Journal of the American Society of Nephrology 2007; 18(-11-):2945-2952. >>> Insulin, porcine; Glycerine, ethanol; SC; Rat; 2004; 12 weeks; Controls received no treatment; long-term study; pumps replaced every 4 wks; multiple pumps per animal (2); animal info (ZSF1, 8 wks old, 200-250 g.); 50% ethanol used.

P8542 Sakai,T., Balasubramanian,K., Maiti,S., Haider,J.B., Schroit,A.J. Plasmin-cleaved beta-2-glycoprotein 1 is an inhibitor of angiogenesis. American Journal of Pathology 2007; 171(-5-):1659-1669. >>> Glycoprotein 1, B-2-; glycoprotein 1, nicked B-2-; SC; IP; Mice; 2002; 28 days; Controls received mp w/ HSA; cancer (prostate adenocarcinoma); peptides; multiple pumps per animal (2); animal info (male, C57BL/6, 6 weeks old); B2GP1 is also known as apolipoprotein H; mp implanted IP on day 1, SC on day 14, implanted pumps not removed.

P8488 Smith,J.M., Lunga,P., Story,D., Harris,N., Le Belle,J., James,M.F., Pickard,J.D., Fawcett,J.W. Inosine promotes recovery of skilled motor function in a model of focal brain injury. Brain 2007; 130(--):915-925. >>> Inosine; CSF, artificial; CSF/CNS; Rat; 2004; 4 weeks; ALZET Brain Infusion Kit 2 used; multiple pumps per animal (2); post op. care (carprofen, IP); traumatic brain inujury; behavioral study; dental cement used; animal info (male, lister, hooded, 200-300 g).

P8674 Yang,J., Dhawan,V., Morrish,D.W., Kaufman,S. Bimodal effects of chronically administered neurokinin B (NKB) on in vivo and in vitro cardiovascular responses in female rats. REGULATORY PEPTIDES 2007; 143(-1-3-):136-142. >>> Neurokinin B; DMSO; water; IV (inferior vena cava); Rat; 2001D; 2ML1; 5 days, 22 hours; Controls received mp w/ vehicle; functionality of mp verified by blood pressure; dose-response (p. 138); cardiovascular; peptides; multiple pumps per animal (3); animal info (female, Long Evans, 250-300g); neuropeptide; silastic cannula; telemetry implanted IP; 10% DMSO used; (3) 2001D pumps used to achieve a higher concentration for 22 hours.

P7891 Armentero,M.T., Fancellu,R., Nappi,G., Bramanti,P., Blandini,F. Prolonged blockade of NMDA or mGluR5 glutamate receptors reduces nigrostriatal degeneration while inducing selective metabolic changes in the basal ganglia circuitry in a rodent model of Parkinson's disease. NEUROBIOLOGY OF DISEASE 2006; 22(-1-):1-9. >>> MK-801; pyridine, 2-methyl-6-(phenylethynyl)-; Saline; water; SC; Rat; 2ML4; 28 days; Controls received mp w/ saline; dose-response (fig. 1); multiple pumps per animal (2); neurodegenerative (Parkinson's disease) animal info (male, Sprague-Dawley, 250-280grams.); MPEP; 6-OHDA induced striatal lesion.

P8161 Balatsouka,D., Gotfredsen,K., Sorensen,L., Lindh,C.H., Berglundh,T. Effect of systemic administration of nicotine on healing in osseous defects. An experimental study in rabbits. Part II. CLINICAL ORAL IMPLANTS RESEARCH 2006; 17(-5-):488-494. >>> Nicotine hydrogen tartrate salt; Water, sterile; SC; Rabbit; 2ML4; 8 weeks; Controls received mp w/ saline; functionality of mp verified by cotinine plasma levels; long-term study; pumps replaced after 4 weeks; multiple pumps per animal (2); animal info (female, New Zealand white, 9-12 months old, 3-4 kg, osseous defects).

P7954 Billes,S.K., Cowley,M.A. Inhibition of Dopamine and Norepinephrine Reuptake Produces Additive Effects on Energy Balance in Lean and Obese Mice. Neuropsychopharmacology 2006; (--):1-13. >>> Nisoxetine; GBR 12783; Saline; water; DMSO; SC; Mice; 1007D; 2001; 1 week; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (male, C57BL/6J); 10% DMSO in saline; 50% DMSO in water; NE reuptake inhibitor; dopamine reuptake inhibitor.

P8053 Bruno,M.A., Cuello,A.C. Activity-dependent release of precursor nerve growth factor, conversion to mature nerve growth factor, and its degradation by a protease cascade. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-17-):6735-6740. >>> Neuroserpin; GM6001; CSF, artificial, PBS; CSF/CNS (parietal cortex); Rat; 2001; 72 hours; Controls received mp w/ vehicle into contralateral side; enzyme inhibitor (tpa, metalloproteinase); multiple pumps per animal (2); animal info (Fischer 344, 6 month old); Lynch coil with air-oil spacer at mp end of tubing; bilateral infusion cannulae; tissue perfusion (parietal cortex).

P7895 Chen,K., Li,F.H., Li,J., Cai,H.B., Strom,S., Bisello,A., Kelley,D.E., Friedman-Einat,M., Skibinski,G.A., McCrory,M.A., Szalai,A.J., Zhao,A.Z. Induction of leptin resistance through direct interaction of C-reactive protein with leptin. Nature Medicine 2006; 12(-4-):425-432. >>> Leptin, recomb. human; C-reactive protein, human; Saline; SC; Mice; mice (transgenic); 6, 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum concentrations; dose-response (fig.5); peptides; MRI; multiple pumps per animal (2); animal info (wt or ob/ob, 8 wk old, human CRPtg.).

P7681 Colpaert,F.C., Deseure,K., Stinus,L., Adriaensen,H. High-efficacy 5-hydroxytryptamine 1A receptor activation counteracts opioid hyperallodynia and affective conditioning. Journal of Pharmacology and Experimental Therapeutics 2006; 316(-2-):892-899. >>> F13640; morphine, HCl; Saline; SC; Rat; 2ML2; 6 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every two weeks; multiple pumps per animal (2); animal info (male, Sprague-Dawley, 220-240 g); during pump replacement a new incision was made about 1 cm away from the previous incision.

P7815 Goldstein,D.M., Alfredson,T., Bertrand,J., Browner,M.F., Clifford,K., Dalrymple,S.A., Dunn,J., Freire-Moar,J., Harris,S., Labadie,S.S., La Fargue,J., Lapierre,J.M., Larrabee,S., Li,F.J., Papp,E., McWeeney,D., Ramesha,C., Roberts,R., Rotstein,D., San Pablo,B., Sjogren,E.B., So,O.Y., Talamas,F.X., Tao,W., Trejo,A., Villasenor,A., Welch,M., Welch,T., Weller,P., Whiteley,P.E., Young,K., Zipfel,S. Discovery of S-[5-amino-1-(4-fluorophenyl)-1H-pyrazol-4-yl]-[3-(2,3- dihydroxypropoxy)phenyl]methanone (RO3201195), an orally bioavailable and highly selective inhibitor of p38 map kinase. Journal of Medicinal Chemistry 2006; 49(-5-):1562-1575. >>> R03201195; PEG 400; SC; Rat; 14 days; Controls received mp w/ vehicle; functionality of mp verified by plasma concentrations; pumps replaced on day 8; enzyme inhibitor (p38 map kinase); multiple pumps per animal (2); animal info (female, Lewis, 120-150g., rat adjuvant arthritis).

P7646 Hirano,T., Ran,J.M., Adachi,M. Opposing actions of angiotensin II type 1 and 2 receptors on plasma cholesterol levels in rats. Journal of Hypertension 2006; 24(-1-):103-108. >>> Angiotensin II; PD123319; CGP42112A; Saline; SC; Rat; 1007D; 2ML1; 2ML2; 2 weeks; 7 days; Controls received mp w/ vehicle; no stress (see pg.105); cardiovascular; peptides; multiple pumps per animal (2); animal info (male, Wistar, 8 wk old, 400 g); Ang II plasma levels taken.

P7592 Imamura,K., Morii,H., Nakadate,K., Yamada,T., Mataga,N., Watanabe,Y., Mori,N. Brain-derived neurotrophic factor enhances expression of superior cervical ganglia clone 10 in lateral geniculate nucleus and visual cortex of developing kittens. European Journal of Neuroscience 2006; 23(-3-):637-648. >>> Brain-derived neurotrophic factor, recomb. human; PBS; BSA; CSF/CNS (visual cortex); Cat (kitten); cat; 2002; 14 days; Controls received mp w/ vehicle; brain tissue distribution; peptides; multiple pumps per animal (2); post op. care (ampicillin sodium).

P8958 Li,J.H., Deane,J.A., Campanale,N.V., Bertram,J.F., Ricardo,S.D. Blockade of p38 mitogen-activated protein kinase and TGF-beta1/Smad signaling pathways rescues bone marrow-derived peritubular capillary endothelial cells in adriamycin-induced nephrosis. Journal of the American Society of Nephrology 2006; 17(-10-):2799-2811. >>> SB203580; ALK5; DMSO; water; Mice; 2 weeks; Controls received mp w/ vehicle; dose-response (p. 2801); enzyme inhibitor (p38 MAP Kinase); multiple pumps per animal (2); animal info (male, BALB/C, 6 wks old, 20-25 g.); TGF-B receptor I inhibitor.

P7623 Ohyama,M., Terunuma,A., Tock,C.L., Radonovich,M.F., Pise-Masison,C.A., Hopping,S.B., Brady,J.N., Udey,M.C., Vogel,J.C. Characterization and isolation of stem cell-enriched human hair follicle bulge cells. Journal of Clinical Investigation 2006; 116(-1-):249-260. >>> Uridine, bromodeoxy-; SC; Mice (nude); 1002; 4-6 weeks; Pumps replaced every 2 weeks; multiple pumps per animal (2).

P8382 Pocivavsek,A., Icenogle,L., Levin,E.D. Ventral hippocampal alpha7 and alpha4beta2 nicotinic receptor blockade and clozapine effects on memory in female rats. Psychopharmacology 2006; 188(-4-):597-604. >>> Nicotine ditartrate; MLA; erythroidine, dihydro-beta; Saline, sterile; CSF, artifical; SC; CSF/CNS (ventral hippocampus); Rat; 2004; 2ML4; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (female, Sprague-Dawley, 200-300g.); neurodegenerative (Alzheimer's disease); methyllycaconitine; cannula placement confirmed w/ Chicago sky-blue dye; schizophrenia.

P7862 Ran,J.M., Hirano,T., Fukui,T., Saito,K., Kageyama,H., Okada,K., Adachi,M. Angiotensin II infusion decreases plasma adiponectin level via its type 1 receptor in rats: an implication for hypertension-related insulin resistance. METABOLISM-CLINICAL AND EXPERIMENTAL 2006; 55(-4-):478-488. >>> Angiotensin II, human; PD123319; CGP42112A; Saline; SC; Rat; 1007D; 2ML1; 2ML2; 7, 14 days; Controls received mp w/ vehicle; functionality of mp verified by plasma angiotensin II levels; dose-response (table 1); no stress (see pg. 482); peptides; multiple pumps per animal (2); animal info (male, Wistar, 8 wk. old, 400g.).

P8258 Rosi,S., Vazdarjanova,A., Ramirez-Amaya,V., Worley,P.F., Barnes,C.A., Wenk,G.L. Memantine protects against LPS-induced neuroinflammation, restores behaviorally-induced gene expression and spatial learning in the rat. Neuroscience 2006; 142(-4-):1303-1315. >>> Endotoxin, LPS; memantine; CSF, artificial; SC; CSF/CNS (fourth ventricle); Rat; 2004; 2ML4; 28 days; Controls received mp w/ vehicle; no stress (see pg. 1308); multiple pumps per animal (2); animal info (male, F-344, 3 months old); neurodegenerative (Alzheimer's disease, Multiple Sclerosis, Amyotrophic Lateral Sclerosis, etc.).

P8386 Serralta,A., Barcia,J.A., Ortiz,P., Duran,C., Hernandez,M.E., Alos,M. Effect of intracerebroventricular continuous infusion of valproic acid versus single i.p. and i.c.v. injections in the amygdala kindling epilepsy model. Epilepsy Research 2006; 70(-1-):15-26. >>> Valproic acid; Saline; CSF/CNS; Rat; 2001; 2002; 7 days; Controls received no treatment; dose-response (fig. 1); comparison of icv, IP injections vs. mp; no stress (see pg. 22); toxicology; multiple pumps per animal (2); animal info (male, Wistar, amygdala-kindled epilepsy, 380-420g.); mp primed 1 hr. in 37.5 celsius saline; evans blue dye used to confirm cannula placement, "chronic i.c.v. VPA infusion affords protection against kindled seizures accompanied by less ataxia and sedation" (pg. 24).

P7927 Tiwari,S., Packer,R.K., Hu,X.Q., Sugimura,Y., Verbalis,J.G., Ecelbarger,C.A. Increased renal alpha-ENaC and NCC abundance and elevated blood pressure are independent of hyperaldosteronism in vasopressin escape. American Journal of Physiology-Renal Physiology 2006; 291(-1-):F49-F57. >>> Vasopressin, 1-desamino-8-D-arginine; aldosterone; SC; Rat; 2002; ; Blood pressure taken; replacement therapy (adrenalectomy); multiple pumps per animal (2); animal info (male, Sprague-Dawley, 275 grams, ADX); dDAVP; one pump for each drug.

P7760 Waeckel,L., Bignon,J., Liu,J.M., Markovits,D., Ebrahimian,T.G., Vilar,J., Mees,B., Blanc-Brude,O., Barateau,V., Le ricousse-Roussanne,S., Duriez,M., Tobelem,G., Wdzieczak-Bakala,J., Levy,B.I., Silvestre,J.S. Tetrapeptide AcSDKP induces postischemic neovascularization through monocyte chemoattractant protein-1 signaling. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2006; 26(-4-):773-779. >>> Peptide, AcSKDP; monocyte chemoattractant protein-1; SC; Mice; 2001; 2004; 7, 21 days; Controls received mp w/ saline; replacement therapy (MCP-1 deficient); dose-response (fig. 1); cardiovascular; peptides; multiple pumps per animal (2); ischemia (hind limb); animal info (c57BI/6, wt or MCP-1 deficient, 10 wk old).