References on the Administration of Agents to Nerves Using ALZET® Osmotic Pumps

16119 Favero, M., Cangiano, A., Busetto, G. The timing of activity is a regulatory signal during development of neural connections. J Mol Neurosci 2014; 53(-3-):324-9. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); rat; animal info: rat strain AO; functionality of mp verified by establishment of a chronic conduction block; tissue perfusion (sciatic nerve);.

15197 Buys, M.J., Alphonso, C., Buys, M.J. Novel Use of Perineural Pregabalin Infusion for Analgesia in a Rat Neuropathic Pain Model1809. ANESTHESIA AND ANALGESIA 2014; 119(--):481-488. >>> Pregabalin; Saline; CSF/CNS (sciatic nerve); Rat; 2ML1; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 8-9 weeks old); behavioral testing (Hargreaves'radient heat testing, guarding behavior, incapacitance testing); pump and catheter secured using 4-0 silk suture; sciatic nerve injury;.

15150 Yan, J.G., Logiudice, J., Davis, J., Zhang, L.L., Agresti, M., Sanger, J., Matloub, H.S., Havlik, R., Yan, J.G. CALCITONIN PUMP IMPROVES NERVE REGENERATION AFTER TRANSECTION INJURY AND REPAIR. MUSCLE&NERVE 2015; 51(--):229-234. >>> Calcitonin; Water, distilled sterile; CSF/CNS (sciatic nerve); Rat; 2006; Animal info (Sprague Dawley, 250-300g, 3 months old); half-life (p.233); long-term study;“To achieve a continuous and gradual mode of delivery, a mini-osmotic pump was implanted to deliver medication at a constant 0.15 ul/h” pg 233;"Calcitonin has short absorption and elimination half-lives of 10–15 minutes and 50–80 minutes, respectively; however, using an osmotic pump allows for gradual and prolonged release."pg233; pg230 diagram of pump implantation;.

15142 Williams, B.A., Butt, M.T., Zeller, J.R., Coffee, S., Pippi, M.A., Williams, B.A. Multimodal Perineural Analgesia with Combined Bupivacaine-Clonidine-Buprenorphine-Dexamethasone: Safe In Vivo and Chemically Compatible in Solution. PAIN MEDICINE 2015; 16(--):186-198. >>> Bupivacaine; clonidine; dexamethasone; Saline; CSF/CNS (sciatic nerve); Rat; 2ML1; Controls received mp w/ vehicle; animal info (male, albino, CD[SD]); no stress (see pg. 192); post op. care (IM butorphanol tartrate 0.05 mg/kg, ceftiofur sodium 5 mg/kg); stability verified by (pg. 195); used polyurethane catheter 0.5mm ID 0.9 mmOD; pumps removed after 1 week; dose (66.6 ug/mL).

14261 Lee, J.Y., Jahng, J.W., Kim, S.M., Kim, M.J., Lee, J.H., Lee, J.H. Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study. Journal of Oral and Maxillofacial Surgery 2015; 73(--):410-423. >>> Nerve growth factor, human B-; PBS; CSF/CNS (inferior alveolar nerve); Dog (beagle); 2ML2; Controls received mp w/ vehicle; animal info (male, beagle, 18 weeks old, 10-12 kg); good methods (picture of implant pg 413); Multiple pumps per animal (2; one pump delivered NGF other delivered PBS); used rat jugular catheter, 15 cm long; pump body placed into retromandibular area; long-term study;.

13925 Schuh, C.D., Pierre, S., Weigert, A., Weichand, B., Altenrath, K., Schreiber, Y., Ferreiros, N., Zhang, D.D., Suo, J., Treutlein, E.M., Henke, M., Kunkel, H., Grez, M., Nuesing, R., Bruene, B., Geisslinger, G., Scholich, K. Prostacyclin mediates neuropathic pain through interleukin 1beta-expressing resident macrophages. Pain 2014; 155(-3-):545-555. >>> CAY10441; PBS; DMSO; CSF/CNS (sciatic nerve); Mice; 1007D; Controls received mp w/ vehicle; animal info (C57BL6N or IP receptor KO mice); 1% DMSO used; behavioral testing (paw withdrawal latency); immunology; sciatic nerve injury; used RenaSil tube attached to PE-60, and fixed by Stabiloblast 2-component glue; pumps primed in 37C saline; pumps implanted subcutaneously and fixed with 6-0 Prolene suture to muscle; CAY10441 is an IP receptor antagonist;.

13630 Unezaki, S., Katano, T., Hiyama, T.Y., Tu, N.H., Yoshii, S., Noda, M., Ito, S. Involvement of Na(x) sodium channel in peripheral nerve regeneration via lactate signaling. European Journal of Neuroscience 2014; 39(-5-):720-729. >>> Lactate; hydroxycinnamic acid, alpha-cyano-4;; Saline; CSF/CNS (sciatic nerve); Mice (transgenic); 1004; Controls received mp w/ vehicle; animal info ([B6.Cg-Tg (thy1-YFP)16Jrs/J, Nax -/-, 8-15 weeks old); behavioral testing (von frey filaments, neurometer analysis); alpha-cyano-4 hydroxycinnamic acid aka CIN; used catheter tubing #7701 to attach pump to silicone tubing sutured to nerve stump; schematic of surgery on pg.725, figure 3A;.

13425 Yan, J.G., Zhang, L.L., Agresti, M., LoGiudice, J., Yan, Y.H., Wang, Z.Y., Sanger, J.R., Matloub, H.S. The effect of calcium modulating agents on peripheral nerve recovery after crush. Journal of Neuroscience Methods 2013; 217(-1-2-):54-62. >>> Nifedipine; calcitonin; CSF/CNS (sciatic nerve); Rat; 2006; Controls received mp w/ saline or sham only; animal info (3 month old, male, Sprague-Dawley 250-300g); functionality of mp verified by decrease in calcium levels; peptides; Picture of MP p56, Fig1A. MP Pump setup p56, Fig1B;.

12973 Ribeiro-Resende, V.T., Carrier-Ruiz, A., Lemes, R.M.R., Reis, R.A.M., Mendez-Otero, R. Bone marrow-derived fibroblast growth factor-2 induces glial cell proliferation in the regenerating peripheral nervous system. Molecular Neurodegeneration 2012; 7(-;-):U1-U17. >>> Antibody, Fibroblast growth factor-2, mouse, monoclonal; CSF/CNS (sciatic nerve); Rat; 2002; Animal info (male, Lister Hooded, 3 mo old).

12923 Gill, B.C., Balog, B.M., Dissaranan, C., Jiang, H.H., Steward, J.B., Lin, D.L., Damaser, M.S. Neurotrophin therapy improves recovery of the neuromuscular continence mechanism following simulated birth injury in rats. NEUROUROLOGY AND URODYNAMICS 2013; 32(-1-):82-87. >>> Albumin; Saline, sterile; CSF/CNS (pudendal nerve); Rat; 1007D; 2002; Animal info (Sprague Dawley, female, virgin, 200-225 g); bilateral cannula used;"localized continuous neurotrophin supplementation has been most effective at promoting nerve regeneration and provided the rationale behind utilizing miniature osmotic pumps in this study."pg 86; bilateral infusion;.

12833 Kim, J.M., Kim, Y.J., Kim, D.M. Increased expression of oxyproteins in the optic nerve head of an in vivo model of optic nerve ischemia. BMC Ophthalmology 2012; 12(-;-):U1-U6. >>> Endothelin-1; CSF/CNS (optic nerve, perineural region); Rabbit; Animal info (New Zealand, 2.5-3.5 kg); long-term study; PE tubing used.

12699 Carrasco, D.I., Bichler, E.K., Rich, M.M., Wang, X.Y., Seburn, K.L., Pinter, M.J. Motor terminal degeneration unaffected by activity changes in SOD1(G93A) mice; a possible role for glycolysis. NEUROBIOLOGY OF DISEASE 2012; 48(-1-):132-140. >>> Tetrodotoxin; dexamethasone; CSF/CNS (sciatic nerve); Mice; 2002; Animal info (SOD1 G93A); good methods, pg 133.

12443 Favero, M., Busetto, G., Cangiano, A. Spike timing plays a key role in synapse elimination at the neuromuscular junction. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2012; 109(-25-):E1667-E1675. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); Rat; 2ML4; Animal info (AO strain, 200-400 g, Harlan); fig 1, schematic of pump and placement of conduction block.

12069 Martinez, J.A., Kasamatsu, M., Rosales-Hernandez, A., Hanson, L.R., Frey, W.H., Toth, C.C. Comparison of central versus peripheral delivery of pregabalin in neuropathic pain states. Molecular Pain 2012; 8(-;-):U7-U26. >>> Pregabalin, I125; pregabalin, unlabeled; CSF/CNS (intrathecal); CSF/CNS (sciatic nerve); Rat; 2002; Controls received mp w/ saline; animal info (Sprague Dawley, male, 200-225 g); catheter patency and functionality of mp verified via pump infusion of india ink; neuropathic pain.

11706 Wang, X.Y., Wang, Q.B., Engisch, K.L., Rich, M.M. Activity-Dependent Regulation of the Binomial Parameters p and n at the Mouse Neuromuscular Junction In Vivo. Journal of Neurophysiology 2010; 104(-5-):2352-2358. >>> Tetrodotoxin; NaCl; CSF/CNS (sciatic nerve); Mice; 1002; Animal info (unaffected litter mates of ClCn1adr-mto2J, 2-4 mo old); catheter tubing was filled with saline so that"after surgery the foot muscles were not paralyzed"pg 2353.

11658 Wang, X.Y., Wang, Q.B., Yang, S.Z., Bucan, M., Rich, M.M., Engisch, K.L. Impaired Activity-Dependent Plasticity of Quantal Amplitude at the Neuromuscular Junction of Rab3A Deletion and Rab3A Earlybird Mutant Mice. Journal of Neuroscience 2011; 31(-10-):3580-3588. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); Mice; Animal info (Rab3a +/-, male, female, 2.5-3.5 mo old, C57BL/6J); silastic cuff used.

11633 Toledo, R.N., Borin, A., Cruz, O.L.M., Ho, P.L., Testa, J.R.G., Fukuda, Y. The Action of Topical Basic Fibroblast Growth Factor in Facial Nerve Regeneration. OTOLOGY&NEUROTOLOGY 2010; 31(-3-):498-505. >>> Fibroblast growth factor, basic; albumin, human; Ringer lactate solution; CSF/CNS (facial nerve anastomosis); Rat; 2002; Controls received mp w/ sodium heparin, human albumin in Ringer lactate solution; animal info (male, Wistar, adult); functionality of mp verified by residual volume; good methods, pg 499; stress/adverse reaction: (see pg. 501)"neuroma at the anastomosis","hemoatoma at the surgical incision"; tissue perfusion (epineural anastomosis); vinyl catheter used;"We chose to use minipumps because this method allows better control of the quantity of bFGF delivered at the target site and is compatible with standard facial nerve surgical techniques. Furthermore, the isolate reservoir of the minipumps prevents the contact of the study drug with substances that could inactivate it"pg 502.

11227 Aktas, Z., Gurelik, G., Goecuen, P.U., Akyuerek, N., Oenol, M., Hasanreisoglu, B. Matrix metalloproteinase-9 expression in retinal ganglion cell layer and effect of topically applied brimonidine tartrate 0.2% therapy on this expression in an endothelin-1-induced optic nerve ischemia model. International Ophthalmology 2010; 30(-3-):253-259. >>> Endothelin-1, human, porcine; Eye (optic nerve); Rabbit; 2002; Animal info (New Zealand, albino); vinyl tubing used; image of pump and tubing through the upper eyelid (Fig.1, Fig 2b);.

11149 Kasselman, L.J., Rutkove, S.B. APPLICATION OF ANGIOTENSIN II TO HEALTHY RAT SCIATIC NERVE CAN PRODUCE NEUROPATHY WITHOUT ASSOCIATED VASCULOPATHY. Muscle&Nerve 2010; 42(-6-):959-965. >>> Angiotensin II; CSF/CNS (sciatic nerve); Rat; 2004; Controls received mp w/ PBS; animal info (male, Wistar, 250-300 g); peptides; long-term study; good methods, pg 960; no stress (see pg. 961)"All animals from both groups survived the surgery without complications"; pump connected to silicone tube that was connected to a piece of Gelfoam; Fig. 1, schematic of pump, tube, and gelfoam. pg 960.

10999 Lee, H.K., Seo, I.A., Suh, D.J., Park, H.T. Nidogen Plays a Role in the Regenerative Axon Growth of Adult Sensory Neurons Through Schwann Cells. Journal of Korean Medical Science 2009; 24(-4-):654-659. >>> Alkaline phosphatase tag; eTEM-AP, recomb.; CSF/CNS (sciatic nerve); Rat; 1002; Controls received mp w/ alkaline phosphatase tag; animal info (adult, male, Sprague-Dawley, 150-300 g); peptides.

10768 Toth, C.C., Willis, D., Twiss, J.L., Walsh, S., Martinez, J.A., Liu, W.Q., Midha, R., Zochodne, D.W. Locally Synthesized Calcitonin Gene-Related Peptide Has a Critical Role in Peripheral Nerve Regeneration. Journal of Neuropathology and Experimental Neurology 2009; 68(-3-):326-337. >>> Cycloheximide; puromycin; Saline; CSF/CNS (sciatic nerve); Rat; 2002; Animal info (adult, male, Sprague-Dawley, 250-300 g).

10685 Vrbova, G., Mehra, N., Shanmuganathan, H., Tyreman, N., Schachner, M., Gordon, T. Chemical communication between regenerating motor axons and Schwann cells in the growth pathway. European Journal of Neuroscience 2009; 30(-3-):366-375. >>> Gallamine triethiodide; tubocurarine; atropine; suramin; CSF/CNS (sciatic nerve); Rat; 2ML4; Controls received mp w/saline; animal info (female, adult, Sprague Dawley, 200-220 g); schematic illustration of pump with silastic catheter, Fig 1b.

10402 Apel, P.J., Ma, J.J., Callahan, M., Northam, C.N., Alton, T.B., Sonntag, W.E., Li, Z.Y. EFFECT OF LOCALLY DELIVERED IGF-1 ON NERVE REGENERATION DURING AGING: AN EXPERIMENTAL STUDY IN RATS. Muscle&Nerve 2010; 41(-3-):335-341. >>> Insulin-like growth factor-I, recomb. human; CSF/CNS (tibial nerve); Rat; 2004; Controls received mp w/ saline; long-term study; pumps replaced after 6 weeks; animal info (Fischer 344 x Brown Norway, Sprague Dawley; 8 months old, 24 months old); diagram of pump with custom-made T-tube.

10381 Favero, M., Buffelli, M., Cangiano, A., Busetto, G. THE TIMING OF IMPULSE ACTIVITY SHAPES THE PROCESS OF SYNAPTIC COMPETITION AT THE NEUROMUSCULAR JUNCTION. Neuroscience 2010; 167(-2-):343-353. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 2002; 2ML4; Animal info (adult, male, Wistar, 180-350 g).

10052 Unezaki, S., Yoshii, S., Mabuchi, T., Saito, A., Ito, S. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. Journal of Neuroscience Methods 2009; 178(-2-):308-315. >>> Nerve growth factor; glial-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Mice (transgenic); 1004; Controls received mp w/ vehicle; half-life (p. 308)"short"; animal info (10 wks old, 20 g., Thy1-YEP); image of pump pg. 309; schematic of drug delivery system with pump+silicone, fig. 1);"Because of the short biological half-life of neurotrophic factors, a delivery system that protects the protein and slowly releases it locally over a prolonged period of time is required."pg. 308; tissue perfusion (sciatic nerve).

9840 Wang, X., LeVatte, T.L., Archibalda, M.L., Chauhan, B.C. Increase in endothelin B receptor expression in optic nerve astrocytes in endothelin-1 induced chronic experimental optic neuropathy. Experimental Eye Research 2009; 88(-3-):378-385. >>> Endothelin-1; CSF/CNS (optic nerve); Rat; 2004; Post op. care (buprenorphine); animal info (adult, male, Brown Norway, 250-300g); fellow eye served as untreated control; silastic tubing used; brain tissue distribution.

9693 Temporin, K., Tanaka, H., Kuroda, Y., Okada, K., Yachi, K., Moritomo, H., Murase, T., Yoshikawa, H. Interleukin-1 beta promotes sensory nerve regeneration after sciatic nerve injury. Neuroscience Letters 2008; 440(-2-):130-133. >>> Interleukin-1, beta; CSF/CNS (sciatic nerve); Rat; 2002; Controls received mp w/ PBS; animal info (female, Wistar, 180-220 g., sciatic nerve injury); behavioral testing (motor function, toe spreading test, sensory function).

9689 Song, X.Y., Li, F., Zhang, F.H., Zhong, J.H., Zhou, X.F. Peripherally-Derived BDNF Promotes Regeneration of Ascending Sensory Neurons after Spinal Cord Injury. PLoS One 2008; 3(-3-):U22-U37. >>> Brain-derived neurotrophic factor, recomb. human; CSF/CNS (sciatic nerve); Rat; 1007D; Controls received mp w/ PBS; comparison of foot pad injections vs. mp; peptides; animal info (female, Sprague Dawley, 8-10 wks old, SCI); behavioral testing (hind limb motor function); spinal cord injury.

9555 Favero, M., Jiang, D.J., Chiamulera, C., Cangiano, A., Fumagalli, G.F. Expression of small-conductance calcium-activated potassium channels (SK3) in skeletal muscle: regulation by muscle activity. JOURNAL OF PHYSIOLOGY-LONDON 2008; 586(-19-):4763-4774. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 2002; Animal info (male, Wistar, 300-350 g., soleus nerve crushed); silicone cuff placed around the sciatic nerve connected to mp by catheter.

9467 Hashimoto, M., Ishii, K., Nakamura, Y., Watabe, K., Kohsaka, S., Akazawa, C. Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury. Journal of Neurochemistry 2008; 107(-4-):918-927. >>> Cyclopamine; Cyclodextrin, 2-hydroxypropyl-b-; CSF/CNS (sciatic nerve); Rat; 2001; Controls received mp w/ vehicle to contralateral side; multiple pumps per animal (2); animal info (male, Wistar, 7 wks old, 200-250 g., sciatic nerve injury); 45% cyclodextrin used.

9109 Hontanilla, B., Auba, C., Gorria, O. Nerve regeneration through nerve autografts after local administration of brain-derived neurotrophic factor with osmotic pumps. Neurosurgery 2007; 61(-6-):1268-1274. >>> Brain-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Rat; 1002; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve graft); replacement therapy (axotomy); no stress (see pg. 1270); stress/adverse reaction: (see pg. 1270)"one pump was extruded"; animal info (male, Wistar, 200-250g.); pg. 1269, fig A shows image of pump implantation and cannulae location.

9041 Li, L.H., Qin, H.Z., Shi, W.Q., Gao, G.D. Local Nogo-66 administration reduces neuropathic pain after sciatic nerve transection in rat. Neuroscience Letters 2007; 424(-3-):145-148. >>> Glutathione S-transferase; glutathione S-transferase-Nogo-66; CSF/CNS (sciatic nerve); Rat; 2002; Controls received mp w/ GST or no treatment; peptides; animal info (male, Sprague Dawley, 240-260g., Sciatic nerve transection); pain; silicon tube used.

8947 Brooks, D.E., Komaromy, A.M., Kallberg, M.E., Miyabashi, T., Ollivier, F.J., Lambrou, G.N. Blood flow velocity response of the ophthalmic artery and anterior optic nerve head capillaries to carbogen gas in the rhesus monkey model of optic nerve head ischemia. VETERINARY OPHTHALMOLOGY 2007; 10(-;-):20-27. >>> Endothelin-1; CSF/CNS (optic nerve); Monkey (Rhesus); 2004; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; peptides; animal info (young, male, Rhesus); glaucoma.

8927 Favero, M., Lorenzetto, E., Bidoia, C., Buffelli, M., Busetto, G., Cangiano, A. Synapse formation and elimination: Role of activity studied in different models of adult muscle reinnervation. Journal of Neuroscience Research 2007; 85(-12-):2610-2619. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 2002; 2ML4; Controls received no treatment; animal info (male, Wistar, 180-400g); silicone nerve cuff used.

8882 Bichler, E.K., Carrasco, D.I., Rich, M.M., Cope, T.C., Pinter, M.J. Rat motoneuron properties recover following reinnervation in the absence of muscle activity and evoked acetylcholine release. JOURNAL OF PHYSIOLOGY-LONDON 2007; 585(-1-):47-56. >>> Tetrodotoxin; Penicillin G potassium; gentamicin sulfate; CSF/CNS (posterior tibial nerve); Rat; 2004; Controls received mp w/ saline; long-term study; pumps replaced after 28 days; no stress (see pg. 52); animal info (female, Wistar, 270-380g, MG nerve crush; used silastic nerve cuff.

8880 Matute, C., Torre, I., Perez-Cerda, F., Perez-Samartin, A., Alberdi, E., Etxebarria, E., Arranz, A.M., Ravid, R., Rodriguez-Antigueedad, A., Saanchez-Goomez, M.V., Domercq, M. P2X7 receptor blockade prevents ATP excitotoxicity in oligodendrocytes and ameliorates experimental autoimmune encephalomyelitis. Journal of Neuroscience 2007; 27(-35-):9525-9533. >>> Adenosine triphosphate-gamma-S; adenosine triphosphate, 2', 3'-O-(benzoyl-4-benzoyl)-; adenosine triphosphate, oxidized; dye, brilliant blue G; Saline; PBS, sterile; CSF/CNS (optic nerve); Rabbit; 1003D; Controls received mp w/ vehicle; neurodegenerative (multiple sclerosis).

8783 VanSaun, M., Hurnburg, B.C., Arnett, M.G., Pence, M., Aferle, M.J. Activation of matrix metal loproteinase-3 is altered at the frog neuromuscular junction following changes in synaptic activity. Developmental Neurobiology 2007; 67(-11-):1488-1497. >>> Tetrodotoxin; Ringer's solution; CSF/CNS (sciatic nerve); Frog (rana pipiens); 2001; Pump implanted SC in the back with catheter guided to the sciatic nerve.

8733 Lever, I.J., Pheby, T.M., Rice, A.S.C. Continuous infusion of the cannabinoid WIN 55,212-2 to the site of a peripheral nerve injury reduces mechanical and cold hypersensitivity. British Journal of Pharmacology 2007; 151(-2-):292-302. >>> WIN-55,212-2 mesylate salt; SR141716A; SR144528; Saline; tween 20; albumin, rat serum; DMSO; CSF/CNS (sciatic nerve); Rat; 2001; Controls received mp w/ vehicle; functionality of mp verified after removal, as well as mp/catheter connection, catheter patency and position; dose-response (fig. 3); animal info (male, Wistar, 250-300 g, partial ligation injury); 4% DMSO; 14.5 % DMSO.

8617 Xie, W.R., Strong, J.A., Li, H.Q., Zhang, J.M. Sympathetic sprouting near sensory neurons after nerve injury occurs preferentially on spontaneously active cells and is reduced by early nerve block. Journal of Neurophysiology 2007; 97(-1-):492-502. >>> Tetrodotoxin; tetraethylammonium chloride; pyridine, 4-amino-; CSF, artificial; CSF/CNS (sciatic nerve); Rat; 2001; Controls received mp w/ vehicle, or contralateral uninjured nerve; replacement therapy (axotomy); animal info (female, Sprague-Dawley, 80-100g., sciatic nerve ligation/transesction); mp primed 4 hrs in 37 celsius saline; chronic pain model.

8253 Wang, X.Y., Engisch, K.L., Teichert, R.W., Olivera, B.M., Pinter, M.J., Rich, M.M. Prolongation of evoked and spontaneous synaptic currents at the neuromuscular junction after activity blockade is caused by the upregulation of fetal acetylcholine receptors. Journal of Neuroscience 2006; 26(-35-):8983-8987. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Mice; 1002; Controls received mp w/ vehicle; animal info (2-4 months old); silastic nerve cuff; tissue perfusion (sciatic nerve).

8188 Wang, Y.L., Wang, D.Z., Nie, X., Lei, D.L., Liu, Y.P., Zhang, Y.J., Suwa, F., Tamada, Y., Fang, Y.R., Jin, Y. The role of bone morphogenetic protein-2 in vivo in regeneration of peripheral nerves. BRITISH JOURNAL OF ORAL&MAXILLOFACIAL SURGERY 2007; 45(-3-):197-202. >>> Bone morphogenic protein-2; Saline; CSF/CNS (facial nerve); Rabbit; 2ML4; Controls received mp w/ vehicle; animal info (New Zealand, 1.6-2.3 kg); total daily infused volume incorrect; tissue perfusion (facial nerve).

8146 Toth, C., Brussee, V., Martinez, J.A., McDonald, D., Zochodne, D.W. Rescue and regeneration of injured peripheral nerve axons by intrathecal insulin. Neuroscience 2006; 139(-2-):429-449. >>> Insulin; Saline; CSF/CNS (intrathecal, lumbar, peroneal nerve); Rat; 2002; Controls received mp w/ vehicle; peptides; animal info (adult male, Sprague-Dawley, 250-300 grams); silicone catheters (0.012"x 0.025") used between L6-S1 vertebrae; nerve perfusion chamber used for nerve infusion; nerve injury; India Ink in pumps to verify delivery; behavioral testing.

7913 Fansa, H., Schneider, W., Wolf, G., Keilhoff, G. Influence of insulin-like growth factor-I (IGF-I) on nerve autografts and tissue-engineered nerve grafts. Muscle Nerve 2002; 26(-1-):87-93. >>> Insulin-like growth factor I; Dulbecco's mem; CSF/CNS (sciatic nerve); Rat; 2004; Controls received mp w/ vehicle; no stress (see pg. 89); animal info (female, wistar, 250-300 g).

7814 Kadoya, T., Oyanagi, K., Kawakami, E., Hasegawa, M., Inagaki, Y., Sohma, Y., Horie, H. Oxidized galectin-1 advances the functional recovery after peripheral nerve injury. Neuroscience Letters 2005; 380(-3-):284-288. >>> Galectin-1, recomb. human oxidized; CSF/CNS (sciatic nerve); Rat; 2ML4; Controls received mp w/ PBS; dose-response (fig. 1); long-term study; no stress (see pg. 285); peptides; animal info (male, adult, Sprague-Dawley);"No rats showed any toxic effects or reaction to the administration of rh GAL-1/Ox."(p. 285).

7663 Domercq, M., Etxebarria, E., Perez-Samartin, A., Matute, C. Excitotoxic oligodendrocyte death and axonal damage induced by glutamate transporter inhibition. Glia 2005; 52(-1-):36-46. >>> Oligonucleotide, antisense; oligonucleotide sense; kainate, dihydro-; aspartic acid, DL-threo-B-benzyloxy-; Saline, sterile; CSF/CNS (optic nerve); Rabbit; 1003D; Controls received mp w/ vehicle, or contralateral nerves; antisense (glutamate transporters GLAST + GLT-1); animal info (adult, male, white New Zealand).

7626 Xie, W.R., Strong, J.A., Meij, J.T.A., Zhang, J.M., Yu, L. Neuropathic pain: Early spontaneous afferent activity is the trigger. Pain 2005; 116(-3-):243-256. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 1003D; 2001; Controls received mp w/ vehicle; animal info (male, wistar, 200-250 g); nerve injury; silicone tubing (0.64 mm I.D., 1.19 mm O.D.);"implanting a silicone cuff on injured sciatic nerve was extremely difficult to do without further damaging the nerve.""hence instead of a cuff we surrounded the nerve with two soft pieces of silicone formed by cutting a slit in the end of silicone tubing."P. 245; behavioral study.

7622 Vassias, I., Lecolle, S., Vidal, P.P., de Waele, C. Modulation of GABA receptor subunits in rat facial motoneurons after axotomy. MOLECULAR BRAIN RESEARCH 2005; 135(-1-2-):260-275. >>> Colchicine; tetrodotoxin; PBS; CSF/CNS (facial nerve); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Long-Evans, 200 g); silastic tubing cuff.

7438 Eleore, L., Vassias, I., Vidal, P.P., Triller, A., de Waele, C. Modulation of glycine receptor subunits and gephyrin expression in the rat facial nucleus after axotomy. European Journal of Neuroscience 2005; 21(-3-):669-678. >>> Colchicine; tetrodotoxin; PBS; CSF/CNS (facial nerve); Rat; 2002; Controls received mp w/ vehicle; facial palsy; long-term study.

7411 Perrin, F.E., Lacroix, S., viles-Trigueros, M., David, S. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration. Brain 2005; 128(--):854-866. >>> Antibody, anti-MCP-1/MIA-1a; antibody, anti-IL-1B; immunoglobulin G; CSF/CNS (sciatic nerve); Mice; 1007D; Controls received mp w/ IgG; animal info (adult female, BALB/c); tissue perfusion (sciatic nerve); PE tubing + silastic nerve cuff used.

7234 Wang, X.Y., Engisch, K.L., Li, Y.J., Pinter, M.J., Cope, T.C., Rich, M.M. Decreased synaptic activity shifts the calcium dependence of release at the mammalian neuromuscular junction in vivo. Journal of Neuroscience 2004; 24(-47-):10687-10692. >>> Tetrodotoxin; Sodium chloride; penicillin; streptomycin; CSF/CNS (sciatic nerve); Mice; 1002; Controls received mp w/ vehicle; TTX cuff made from silastic tubing, was placed around sciatic nerve and connected to mp; tissue perfusion (sciatic nerve).

7152 Motoyoshi, K., Hyodo, M., Yamagata, T., Gyo, K. Restoring vocal fold movement after transection and immediate suturing of the recurrent laryngeal nerve with local application of basic fibroblast growth factor: An experimental study in the rat. Laryngoscope 2004; 114(-7-):1247-1252. >>> Fibroblast growth factor, basic; PBS; BSA; CSF/CNS (recurrent laryngeal nerve); Rat; 2002; Controls received mp w/ vehicle; functionality of mp verified by residual volume; good methods (p. 1248); nerve infusion schematic (p. 1248).

7113 Qiu, J., Cafferty, W.B.J., McMahon, S.B., Thompson, S.W.N. Conditioning injury-induced spinal axon regeneration requires signal transducer and activator of transcription 3 activation. Journal of Neuroscience 2005; 25(-7-):1645-1653. >>> AG-490; Saline; CSF/CNS (sciatic nerve); Rat; 2004; Controls received mp w/ vehicle; enzyme inhibitor (JAK2); silastic tubing and a gortex nerve cuff used.

6969 McCallister, W.V., McCallister, E.L., Dubois, B., Trumble, T.E. Regeneration along intact nerves using nerve growth factor and ciliary neurotrophic factor. Journal of Reconstructive Microsurgery 2004; 20(-6-):473-481. >>> Nerve growth factor; ciliary neurotrophic factor; CSF/CNS (peroneal nerve); Rat; 2ML4; Controls received mp w/ isotonic saline; peptides; mp primed 24 hours in 37 degrees Celsius saline.

6869 Miana-Mena, F.J, Roux, S, Benichou, J.C, Osta, R, Brulet, P  PNAS 2002; 99(-5-):3234-3239. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); Mice; 1007D; Controls received mp w/ sterile saline; tissue perfusion (sciatic nerve).

6752 Buffelli, M., Busetto, G., Bidoia, C., Favero, M., Cangiano, A. Activity-dependent synaptic competition at mammalian neuromuscular junctions. NEWS IN PHYSIOLOGICAL SCIENCES 2004; 19(--):85-91. >>> Tetrodotoxin; CSF/CNS (soleus nerve); Rat; Controls received mp on contralateral side.

6741 Chauhan, B.C., LeVatte, T.L., Jollimore, C.A., Yu, P.K., Reitsamer, H.A., Kelly, M.E.M., Yu, D.Y., Tremblay, F., Archibald, M.L. Model of endothelin-1 - Induced chronic optic neuropathy in rat. INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE 2004; 45(-1-):144-152. >>> Endothelin-1; Balanced salt solution; Eye (retrobulbar optic nerve); Rat; 2004; Controls received mp w/ vehicle and fellow eye w/ no treatment; dose-response (fig.5); long-term study; pumps replaced every 28 days; peptides; post op. care (rentamicin, buprenex).

6698 Brooks, D.E., Kaellberg, M.E., Cannon, R.L., Komaromy, A.M., Ollivier, F.J., Malakhova, O.E., Dawson, W.W., Sherwood, M.B., Kuekuerichkina, E.E., Lambrou, G.X. Functional and structural analysis of the visual system in the rhesus monkey model of optic nerve head ischemia. INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE 2004; 45(-6-):1830-1840. >>> Endothelin; Saline; acetic acid; CSF/CNS (optic nerve); Monkey; Controls received mp w/ vehicle; long-term study; pumps replaced every 6 weeks; no stress (see pg. 1833); peptides;"The osmotic minipumps were well tolerated by the monkeys for the length (1.5 years) of the experiment."The long exposure time was used to"mimic more closely the prolonged human glaucomatous condition."p. 1838; pump model not listed; ischemia.

6635 Trigueros, S.D.A., Kalyvas, A., David, S. Phospholipase A2 plays an important role in myelin breakdown and phagocytosis during Wallerian degeneration. MOLECULAR AND CELLULAR NEUROSCIENCE 2003; 24(-3-):753-765. >>> AACOCF3; PBS; DMSO; CSF/CNS (sciatic nerve); Mice; Controls received mp w/ vehicle; enzyme inhibitor (cytosolic phospolipase); tygon tubing used; AACOCF3 was dissolved in PBS and 1% DMSO; AACOCF3 is an arachidonic acid analogue/cPLA2 inhibitor or (arachidonyl trifluoromethyl ketone); pump model not listed; tissue perfusion (sciatic nerve).

6522 Costanzo, E.M., Barry, J.A., Ribchester, R.R. Competition at silent synapses in reinnervated skeletal muscle. Neuroscience 2000; 3(-7-):694-700. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; Controls received mp w/ agent and resectioned SN axons; no stress (see pg. 697, 699);"the advantage of this technique is that it does not cause nerve damage...while restricting the rapid diffusion of TTX from its site of administration and thereby ensuring patent, reliable and long-lasting nerve conduction block."(p. 699).

6351 Dobretsov, M., Hastings, S.L., Romanovsky, D., Stimers, J.R., Zhang, J.M. Mechanical hyperalgesia in rat models of systemic and local hyperglycemia. Brain Research 2003; 960(-1-2-):174-183. >>> Glucose; sorbinil; HEPES, buffered saline; CSF/CNS (sciatic nerve); Rat; 2002; Enzyme inhibitor (aldose reductase); post op. care (amoxicillin/clavulanate potassium 3 days).

6135 Dupont, A.C., Richmond, F.J.R., Loeb, G.E. Prevention of muscle disuse atrophy by low-frequency electrical stimulation in rats. IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING 2003; 11(-3-):218-226. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 1007D; Post Op. care (topical lidocaine cream applied); schematic of pump and nerve cuff p. 220;"all devices were checked at necropsy, and no evidence was found that devices moved from their sites of implantation."p.221.

6133 Dupont, A.C., Richmond, F.J.R., Loeb, G.E. Effects of muscle immobilization at different lengths on tetrodotoxin-induced disuse atrophy. IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING 2003; 11(-3-):209-217. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Rat; 1007D; Post Op. Care (Topical Lidocaine cream over sutures); schematic of pump and nerve cuff p. 210; nerve cuff consisted of silastic tubing&a custom cuff; cuff was closed by looping four sutures (3-0 silk) around it.

6149 Boyd, J.G., Gordon, T. Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneurons in vivo. Experimental Neurology 2003; 183(-2-):610-619. >>> Glial-derived neurotrophic factor; brain-derived neurotrophic factor; Saline; CSF/CNS (tibial nerve); Rat; 2001; 2ML4; Controls received mp w/ vehicle; tissue perfusion (nerve repair site); good methods (p. 611,612); silastic cuff used; pumps used for a 1 week and 4 week study.

5899 Rogers, S.D., Peters, C.M., Pomonis, J.D., Hagiwara, H., Ghilardi, J.R., Mantyh, P.W. Endothelin B receptors are expressed by astrocytes and regulate astrocyte hypertrophy in the normal and injured CNS. Glia 2003; 41(-2-):180-190. >>> Endothelin-1; Bosentan; Dye, Evans blue; CSF, artificial; CSF/CNS (optic nerve); Rabbit; 2ML2; Controls received mp w/ vehicle; functionality of mp verified by Evans Blue Dye; good methods p.182, 181; antihypertensive; Bosentan (commercial drug name is tracleer) is an endothelin receptor antagonist (ERA); Vialon catheter with holes used; pump/catheter schematic (p.182); pump is described as advantageous for optic nerve infusion (p.182).

5863 Fukaya, K., Hasegawa, M., Mashitani, T., Kadoya, T., Horie, H., Hayashi, Y., Fujisawa, H., Tachibana, O., Kida, S., Yamashita, J. Oxidized galectin-1 stimulates the migration of Schwann cells from both proximal and distal stumps of transected nerves and promotes axonal regeneration after peripheral nerve injury. Journal of Neuropathology and Experimental Neurology 2003; 62(-2-):162-172. >>> Galectin-1; Antibody, immunoglobulin G, rabbit; Antibody, anti-galectin-1; PBS; CSF/CNS (sciatic nerve); Rat; 2001; Controls received mp w/ vehicle; tissue perfusion (autograft sciatic nerve pg 163); peptides; recomb human galectin-1 used, also called beta-galactosidase binding animal lectin.

5719 Germinario, E., Esposito, A., Midrio, M., Betto, R., Danieli-Betto, D. Expression of sarco(endo)plasmic reticulum Ca2+-ATPase slow (SERCA2) i soform in regenerating rat Soleus skeletal muscle depends on nerve impulses. Experimental Physiology 2002; 87(-5-):575-583. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); rat; 2002; tissue perfusion (nerve cuff); PE catheter connected to a silicon nerve cuff.

5502 Tam, S.L., Archibald, V., Tyreman, N., Gordon, T. Tetrodotoxin prevents motor unit enlargement after partial denervation in rat hindlimb muscles. JOURNAL OF PHYSIOLOGY-LONDON 2002; 543(-2-):655-663. >>> Tetrodotoxin; CSF/CNS (sciatic nerve); Rat; 2ML1; Functionality of mp verified by blockage of the sciatic nerve action potential.

5376 Wongtrakul, S., Bishop, A.T., Friedrich, P.F. Vascular endothelial growth factor promotion of neoangiogenesis in conventional nerve grafts. J Hand Surg. [Am. ] 2002; 27(-2-):277-285. >>> Vascular endothelial growth factor; PBS; Albumin, rabbit serum; CSF/CNS (sciatic nerve); Rabbit; 1003D; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve graft); peptides; human recomb. VEGF used; transplantation;.

5318 Young, C., Miller, E., Nicklous, D.M., Hoffman, J.R. Nerve growth factor and neurotrophin-3 affect functional recovery foll owing peripheral nerve injury differently. RESTORATIVE NEUROLOGY AND NEUROSCIENCE 2001; 18(--):167-175. >>> Nerve growth factor; NT-3; Saline; acetate; CSF/CNS (Sciatic nerve); Rat; 2004; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve); peptides;.

5314 Gordon, T., Boyd, J.G., Gordon, T. A dose-dependent facilitation and inhibition of peripheral nerve regeneration by brain-derived neurotrophic factor. European Journal of Neuroscience 2002; 15(--):613-626. >>> Brain-derived neurotropic factor; PBS; Tween 20; CSF/CNS (tibial nerve); Rat; 2ML4; Controls received gelfoam or mp w/ saline; tissue perfusion (tibial nerve); stability verified by in vitro assay on embryonic chick neurons (p. 614); good methods (p. 614-5); peptides; silastic tubing used; graphic showing nerve perfusion (p. 615);"pump, nerve cuff and drug delivery catheter did not interfere with regeneration of the TIB motor axons"(p. 617).

5140 Tiangco, D.A., Papakonstantinou, K.C., Mullinax, K.A., Terzis, J.K. IGF-I and end-to-side nerve repair: A dose-response study. Journal of Reconstructive Microsurgery 2001; 17(--):247-256. >>> Insulin-like growth factor I; Acetic acid; BSA; CSF/CNS (musculocutaneous nerve); rat; 2004; controls received mp w/ vehicle; tissue perfusion (musculocutaneous nerve coaption site); dose-response (text p. 254 paragraph 3); no stress (see pg. 251); good methods p. 249; vehicle was a 10mM acetic acid solution, with 0.1% BSA; nerve regeneration.

4913 Kryger, G.S., Kryger, Z., Zhang, F., Shelton, D.L., Lineaweaver, W.C., Buncke, H.J. Nerve growth factor inhibition prevents traumatic neuroma formation in the rat.. JOURNAL OF HAND SURGERY-AMERICAN VOLUME 2001; 26A(-4-):635-644. >>> Immunoglobulin G, TrkA; PBS;; CSF/CNS (sciatic nerve);; rat;; 2004;; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve); stress/adverse reaction: (see p. 643) foreign body reaction observed, but did not interfere with pump delivery; peptides; silicone tube used; TrkA-IgG is a nerve growth factor inhibitor; TrkA-IgG consists of the high affinity NGF receptor (TrkA) fused to an IgG heavy chain;.

4841 Everill, B, Kocsis, J.D. Nerve growth factor maintains potassium conductance after nerve injury in adult cutaneous afferent dorsal root ganglion neurons.. Neuroscience 2000; 100(-2-):417-422. >>> Nerve growth factor;; Saline;; CSF/CNS (sciatic nerve);; rat;; 2002;; Controls received mp w/ vehicle; peptides; tissue perfusion (sciatic nerve); mouse NGF used;.

4498 Cormery, B., Pons, F., Marini, J.-F., Gardiner, P.F. Myosin heavy chains in fibers of TTX-paralyzed rat soleus and medial gastrocnemius muscles;. J. Appl. Physiol 2000; 88(--):66-76. >>> Tetrodotoxin;; Penicillin; Streptomycin;; CSF/CNS (sciatic nerve);; rat;; 2004; 2002;; controls received mp with sterile saline; tissue perfusion (nerve cuff);.


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“In preliminary studies using this model, we observed severe side effects with 5-FU doses beyond 25 mg/kg biweekly injections (mice developed skin ulcers, bleeding and severe diarrhea). Thus, we chose to deliver 5-FU by continuous infusion at an equivalent cumulative dose in order to minimize toxicity. Additionally, studies have demonstrated an increased efficacy of chemotherapeutic agents when administered continuously.” Stoeltzing et al. Int. J. Cancer 2003;104:496-503.