References on the Administration of Neurotrophic Factors
Using ALZET® Osmotic Pumps


16332 Sophie Dutheil,, Isabelle Watabe,, Karina Sadlaoud,, Alain Tonetto,, Brahim Tighilet2. BDNF Signaling Promotes Vestibular Compensation by Increasing Neurogenesis and Remodeling the Expression of Potassium-Chloride Cotransporter KCC2 and GABAAReceptor in the Vestibular Nuclei. Journal of Neuroscience 2016; 36(-23-):6199-6212. >>> Brain-derived neurotrophic factor, K252a; Saline; CSF, artificial; CSF/CNS (ventricles); Cat; 2ML4; Controls received mp w/ saline; animal info (adult, male cat, 4-5 kg); dose-response (pg 6205, 6206); behavioral testing (rotating beam experimental device test); peptides;.

16211 Meschin, P., Demion, M., Cazorla, O., Finan, A., Thireau, J., Richard, S., Lacampagne, A. p11 modulates calcium handling through 5-HT(4)R pathway in rat ventricular cardiomyocytes. Cell Calcium 2015; 58(-6-):549-57. >>> Brain-derived neurotrophic factor; saline; SC; rat; 2ML2; Controls received mp w/ vehicle; animal info (200–224 g male Wistar rats); functionality of mp verified by ECG (electrocardiograms); post op. care (lidocaine, heating pad); replacement therapy (neurotrophin); Dose (180 ng/day));.

15870 Ramekers, D., Versnel, H., Strahl, S. B., Klis, S. F., Grolman, W. Temporary Neurotrophin Treatment Prevents Deafness-Induced Auditory Nerve Degeneration and Preserves Function. J Neurosci 2015; 35(-36-):12331-45. >>> Brain-derived neurotrophic factor; PBS; guinea pig serum; CSF/CNS; Guinea pig; 2004; Controls received mp w/ vehicle; animal info (female, albino, 250-350g); functionality of mp verified by histological analysis; 1% guinea pig serum used; stress/adverse reaction: (see pg. 12336); Cannula placement verified via histological analysis; pumps removed after 4 weeks; pumps primed for 40 hours in 37C saline; Dose (17 ug/28 days);.

15794 Wen-Chung Liu, MD, San-Nan Yang, MD, PhD, Chih-Wei J. Wu, PhD, Lee-Wei Chen, MD, PhD, Julie Y.H. Chan, PhD. Hyperbaric Oxygen Therapy Alleviates Carbon Monoxide Poisoning–Induced Delayed Memory Impairment by Preserving Brain-Derived Neurotrophic Factor–Dependent Hippocampal Neurogenesis. Crit. Care Med 2016; 44(-1-):. >>> TrkB-Fc, recombinant human; brain-derived neurotrophic factor; CSF, artificial; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult); animal info (male, Sprague Dawley, adult); behavioral testing (radial arm maze, memory talk); used dental cement; pumps primed in 37C saline prior to implantation; Dose (); brain coordinates;.

15746 Jia Chen, Cheng-Ren Li, Heng Yang, Juan Liu, Tao Zhang, Shu-Sheng Jiao, Yan-Jiang Wang, Zhi-Qiang Xu. proBDNF Attenuates Hippocampal Neurogenesis and Induces Learning and Memory Deficits in Aged Mice. NEUROTOXICITY RESEARCH 2016; (-29-):47-53. >>> Brain-derived neurotrophic factor, pro; antibody brain-derived neurotrophic factor, anti-pro;; BSA; CSF/CNS (hippocampus); Mice; 1002; Controls received mp w/ vehicle; Controls received mp w/ vehicle; neurodegenerative (Alzheimer's Disease); behavioral testing (morris water maze); Cannula placement verified postmortem;.

14928 Garofalo, S., D'Alessandro, G., Chece, G., Brau, F., Maggi, L., Rosa, A., Porzia, A., Mainiero, F., Esposito, V., Lauro, C., Benigni, G., Bernardini, G., Santoni, A., Limatola, C., Limatola, C. Enriched environment reduces glioma growth through immune and non-immune mechanisms in mice. Nature Communications 2015; 6(--):U26-U38. >>> Interleukin-15; brain-derived neurotrophic factor; PBS; CSF/CNS (striatum); Mice; 1007D; Controls received mp w/ vehicle; animal info (male, C57BL6, 3 weeks or 2 months old); ALZET brain infusion kit 3 used; cancer (glioma, U87MG human); tissue perfusion (right striatum); immunology; pumps primed in 37C saline overnight;.

14204 Frias, B., Santos, J., Morgado, M., Sousa, M.M., Gray, S.M.Y., McCloskey, K.D., Allen, S., Cruz, F., Cruz, C.D., Cruz, C.D. The Role of Brain-Derived Neurotrophic Factor (BDNF) in the Development of Neurogenic Detrusor Overactivity (NDO). Journal of Neuroscience 2015; 35(--):2146-2160. >>> Brain-derived neurotropic factor; TrkB-Ig2; Tris buffer; glycerol; CSF/CNS (intrathecal); Rat; 2001; 2004; Controls received mp w/ saline; animal info (female, Wistar, 250-275g); 10% glycerol used; spinal cord injury; no stress (see pg. 2154); post op. care (ciprofloxacin 1 mg/kg IP injection; bladders manually emptied twice a day); used silicone catheter;.

13898 Pettersson, L.M.E., Geremia, N.M., Ying, Z.X., Verge, V.M.K., Pettersson, L.M.E. Injury-Associated PACAP Expression in Rat Sensory and Motor Neurons Is Induced by Endogenous BDNF. PLoS One 2014; 9(--):U629-U640. >>> Antibody, brain-derived neurotrophic factor; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ control IgG; animal info (male, Wistar, 250-300tg); post op. care (buprenorphine 0.05-0.1 mg/kg SC); peripheral nerve injury;.

13417 Mantilla, C.B., Gransee, H.M., Zhan, W.Z., Sieck, G.C. Motoneuron BDNF/TrkB signaling enhances functional recovery after cervical spinal cord injury. Experimental Neurology 2013; 247(-;-):101-109. >>> Brain-derived neurotrophic factor;; CSF/CNS (intrathecal); Rat; 2002; Animal info (male, Sprague Dawley, adult); spinal cord injury; post op. care (Acetominophen orally and buprenorphine IM for 3 days); pulsed delivery (delayed delivery for 3 days - aCSF only in catheter); tissue perfusion (C4 segment of spine); peptides;"Mini-osmotic pumps were successfully implanted for intrathecal delivery at the C4 level and functioned properly throughout the 14 day duration of the experiment."pg 103"Differences in survival rates across groups were likely unrelated to the possible additional morbidity associated with intrathecal catheter and miniosmotic pump implantation, as the survival rate was 70% (28 out of 40) compared to 84% (26 out of 31) in rats not implanted with an intrathecal pump (p = 0.17)."pg 103; PE-10 intrathecal cannula, 10 cm.

13405 Landry, T.G., Fallon, J.B., Wise, A.K., Shepherd, R.K. Chronic neurotrophin delivery promotes ectopic neurite growth from the spiral ganglion of deafened cochleae without compromising the spatial selectivity of cochlear implants. Journal of Comparative Neurology 2013; 521(-12-):2818-2832. >>> Neurotrophin; Brain-derived neurotrophic factor; Ear (cochlea); Guinea pig; 2004; Controls received mp w/ artificial perilymph; animal info (young adult, 300-600g); tissue perfusion (cochlea); peptides;.

13233 Giampa, C., Montagna, E., Dato, C., Melone, M.A.B., Bernardi, G., Fusco, F.R. Systemic Delivery of Recombinant Brain Derived Neurotrophic Factor (BDNF) in the R6/2 Mouse Model of Huntington's Disease. PLoS One 2013; 8(-6-):U444-U455. >>> Brain-derived neurotrophic factor;; PBS; BSA; SC; Mice; 1004; Controls received mp w/ saline; animal info (F1, R6/2); pumps replaced every 28 days; behavioral testing (motor coordination); mp were used to infuse BDNF peripherally to examine its effect centrally;.

12919 Frias, B., Allen, S., Dawbarn, D., Charrua, A., Cruz, F., Cruz, C.D. BRAIN-DERIVED NEUROTROPHIC FACTOR, ACTING AT THE SPINAL CORD LEVEL, PARTICIPATES IN BLADDER HYPERACTIVITY AND REFERRED PAIN DURING CHRONIC BLADDER INFLAMMATION. Neuroscience 2013; 234(-;-):88-102. >>> Brain-derived neurotrophic factor; CSF/CNS (intrathecal); Rat; Control animals received mp w/ saline; animal info (Wistar, female); infusion rate of 1 ul/hr.

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2. Ciliary Neurotrophic Factor

13595 Fang, M.R., He, D.Q., Zhang, F., Hu, Z.Y., Yang, J., Jiang, H., Han, S. Antineuroinflammatory and neurotrophic effects of CNTF and C16 peptide in an acute experimental autoimmune encephalomyelitis rat model. FRONTIERS IN NEUROANATOMY 2013; 7(-;-):U1-U17. >>> Ciliary neurotrophic factor, recombinant rat; PBS; CSF/CNS (intrathecal); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Lewis, 250-300g, adult); ALZET brain infusion kit used; post op. care (temperature and humidity controlled chambers; penicillin injected IM 25000 IU); immunology; Ciliary neurotrophic factor aka CNTF; Ciliary neurotrophic factor is a cytokine; pumps primed overnight in room temp saline; cannulas sterilized overnight in 100% ethanol; used anchoring screws and dental cememnt;.

13585 Baeyens, L., Lemper, M., Leuckx, G., De Groef, S., Bonfanti, P., Stange, G., Shemer, R., Nord, C., Scheel, D.W., Pan, F.C., Ahlgren, U., Gu, G.Q., Stoffers, D.A., Dor, Y., Ferrer, J., Gradwohl, G., Wright, C.V.E., Van de Casteele, M., German, M.S., Bouwens, L., Heimberg, H. Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice. NATURE BIOTECHNOLOGY 2014; 32(-1-):76-+. >>> Epidermal growth factor, human recombinant; ciliary neurotrophic factor, human recombinant; Acetic acid; IP; Mice; 1007D; Controls received mp w/ vehicle; animal info (ALX35d, 13 weeks old, hyperglycemic); diabetes; ciliary neurotrophic factor aka CNTF; Epidermal growth factor aka EGF;.

12225 Solymar, M., Szelenyi, Z., Petervari, E. A Fever-like Effect of Central Infusion of CNTF in Freely Moving Mice with Diet-Induced Obesity. Journal of Molecular Neuroscience 2011; 45(-2-):212-215. >>> Ciliary neurotrophic factor; NaCl; CSF/CNS; Mice; 2001; Animal info (male, C57BL/6); incorrectly listed Model 2001D used.

11330 Fang, M.R., Wang, J., Huang, J.Y., Ling, S.C., Rudd, J.A., Hu, Z.Y., Yew, D.T., Han, S. The Neuroprotective Effects of Reg-2 Following Spinal Cord Transection Injury. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology 2011; 294(-1-):24-45. >>> Regeneration gene protein 2; ciliary neurotrophic factor; Saline; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001D; 2001; Controls received mp w/ vehicle; animal info (adult, female, 200-250 g, Sprague Dawley); functionality of mp verified by residual volume; spinal cord injury.

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3. Glial-derived Neurotrophic Factor

15022 Fukuoka, T., Noguchi, K., Fukuoka, T. A POTENTIAL ANTI-ALLODYNIC MECHANISM OF GDNF FOLLOWING L5 SPINAL NERVE LIGATION; MITIGATION OF NPY UP-REGULATION IN THE TOUCH SENSE PATHWAY. NEUROSCIENCE 2015; 304(--):240-249. >>> Glial cell line-derived neurotrophic factor; Saline; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, young adult, 220-230g); behavioral testing (paw withdrawal);.

13662 Sterky, F.H., Pernold, K., Harvey, B.K., Lindqvist, E., Hoffer, B.J., Olson, L. Glial Cell Line-Derived Neurotrophic Factor Partially Ameliorates Motor Symptoms Without Slowing Neurodegeneration in Mice With Respiratory Chain-Deficient Dopamine Neurons. CELL TRANSPLANTATION 2013; 22(-9-):1529-1539. >>> Glial cell line-derived neurotrophic factor; Saline; CSF/CNS (striatum); Mice; 1004; Controls received mp w/ vehicle; ALZET brain infusion kit used; neurodegenerative (Parkinson); behavioral testing (spontaneous behavior); Used polyethylene tubing instead of vinyl tubing in BIK p. 1531;.

11956 Fransson, A., Maruyama, J., Miller, J.M., Ulfendahl, M. Post-Treatment Effects of Local GDNF Administration to the Inner Ears of Deafened Guinea Pigs. Journal of Neurotrauma 2010; 27(-9-):1745-1751. >>> Glial-derived neurotrophic factor; Ear (cochlea); Guinea pig; 2002; Controls received mp w/ artificial perilymph; animal info (deafened); silcone tube used; tissue perfusion (cochlea); pump replaced after 2 weeks; post op. care (lidocaine).

11718 Patil, S.B., Brock, J.H., Colman, D.R., Huntley, G.W. Neuropathic pain- and glial derived neurotrophic factor-associated regulation of cadherins in spinal circuits of the dorsal horn. Pain 2011; 152(-4-):924-935. >>> Glial-derived neurotrophic factor, recomb. human; Citrate buffer; CSF/CNS (intrathecal); Rat; 1007D; 2002; Controls received mp w/ vehicle; animal info (adult, male, Sprague-Dawley,>60 days old, 200-250 g).

11422 Joseph, N.M., He, S.H., Quintana, E., Kim, Y.G., Nunez, G., Morrison, S.J. Enteric glia are multipotent in culture but primarily form glia in the adult rodent gut. Journal of Clinical Investigation 2011; 121(-9-):3398-3411. >>> Epidermal growth factor; fibroblast growth factor; glial-derived neurotrophic factor; BSA; PBS; IP; Rat; Controls received mp w/ vehicle; animal info (P90).

11029 Voutilainen, M.H., Baeck, S., Peraenen, J., Lindholm, P., Raasmaja, A., Maennistoe, P.T., Saarma, M., Tuominen, R.K. Chronic infusion of CDNF prevents 6-OHDA-induced deficits in a rat model of Parkinson's disease. Experimental Neurology 2011; 228(-1-):99-108. >>> Central dopmaine neurotrophic factor; mesencephalic astrocyte-derived neurotrophic factor; glial-derived neurotrophic factor; PBS; CSF/CNS (striatum); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Wistar, 250-280 g); neuroprotection.

10257 Ubhi, K., Rockenstein, E., Mante, M., Inglis, C., Adame, A., Patrick, C., Whitney, K., Masliah, E. Neurodegeneration in a Transgenic Mouse Model of Multiple System Atrophy Is Associated with Altered Expression of Oligodendroglial-Derived Neurotrophic Factors. Journal of Neuroscience 2010; 30(-18-):6236-6246. >>> Glial-derived neurotrophic factor; CSF/CNS; Mice (transgenic); 1002; Animal info (MBP-h alpha syn Tg, 8 months old).

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4. NT-3

R0239 Richardson,R.T., Noushi,F., O'Leary,S. Inner ear therapy for neural preservation. AUDIOLOGY AND NEURO-OTOLOGY 2006; 11(-6-):343-356. >>> Nerve growth factor; NT-3; adenovirus; brain-derived neurotrophic factor; perilymph, artificial; glial-derived neurotrophic factor; ciliary neurotrophic factor; fibroblast growth factor, acidic; fibroblast growth factor-1; fibroblast growth factor-2; fibroblast growth factor, basic; Ear (cochlea); ear (scala tympani); Guinea-pig; 1,2,4,8 weeks; 15-60, 11-12, 26 days; Comparison of polymers, hydrogels, gene therapy, cell-based therapy, and injections vs. mp; long-term study; pumps replaced; no stress (see pg. 350); half-life (p. 344), short in blood; gene therapy; peptides; animal info (deafened); Table 2; "The mini-osmotic pump device is ideally suited to studying the effects of neurotrophic factors in the cochlea experimentally." (p. 350).

P9183 Wilson-Gerwing,T.D., Stucky,C.L., McComb,G.W., Verge,V.M.K. Neurotrophin-3 significantly reduces sodium channel expression linked to neuropathic pain states. Experimental Neurology 2008; 213(-2-):303-314. >>> NT-3; PBS; albumin, serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Post op. care (buprenorphine); animal info (male, Wistar, young adult, 250-300 g.).

P8038 Wilson-Gerwing,T.D., Verge,V.M.K. Neurotrophin-3 attenuates galanin expression in the chronic constriction injury model of neuropathic pain. Neuroscience 2006; 141(-4-):2075-2085. >>> NT-3; PBS; albumin, rat serum; streptomycin; penicillin; CSF/CNS (intrathecal, lumbar); Rat; 2001; 7 days; Post op. care (buprenorphine); animal info (adult, male, wistar, 250-300 grams).

P7994 Vavrek,R., Girgis,J., Tetzlaff,W., Hebert,G.W., Fouad,K. BDNF promotes connections of corticospinal neurons onto spared descending interneurons in spinal cord injured rats. Brain 2006; 129(--):1534-1545. >>> Brain-derived neurotrophic factor; NT-3; Albumin, rat serum; CSF/CNS (left motor cortex, intrathecal, cisterna magna); Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; SCI (spinal cord injury); dental cement used to secure cannula; NT-3 delivered intrathecally; tissue perfusion (left motor cortex).

P7765 Richardson,R.T., O'Leary,S., Wise,A., Hardman,J., Clark,G. A single dose of neurotrophin-3 to the cochlea surrounds spiral ganglion neurons and provides trophic support. Hearing Research 2005; 204(-1-2-):37-47. >>> NT-3, recomb. human; brain-derived neurotrophic factor; Ringer's solution; Ear (scalatympani); Guinea-pig; 2004; 28 days; Comparison of bolus infusion vs. mp; peptides; animal info (adult, pigmented Dunkin-Hartley, 455g.); tissue perfusion (scala tympani).

P7470 Plantman,S., Novikova,L., Novikov,L., Hammarberg,H., Wallquist,W., Kellerth,J.O., Cullheim,S. Integrin messenger RNAs in the red nucleus after axotomy and neurotrophic administration. NeuroReport 2005; 16(-7-):709-713. >>> Brain-derived neurotrophic factor, recomb human; NT-3; CSF/CNS (intrathecal, subarachnoid space); Rat; 1, 3 weeks; Controls recieved no treatment; peptides; animal info (female, Sprague-Dawley); spinal hemisection (peripheral axotomy).

P7331 Wise,A.K., Richardson,R., Hardman,J., Clark,G., O'Leary,S. Resprouting and survival of guinea pig cochlear neurons in response to the administration of the neurotrophins brain-derived neurotrophic factor and neurotrophin-3. Journal of Comparative Neurology 2005; 487(-2-):147-165. >>> Brain-derived neurotrophic factor, recomb. human; NT-3; Ear (scala tympani); Guinea-Pig; 2004; 28 days; Controls received mp w/ Ringer's solution; cannula patency ascertained by visual inspection; tissue perfusion (scala tympani).

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5. NT-4-5

P8676 Royo,N.C., LeBold,D., Magge,S.N., Chen,I., Hauspurg,A., Cohen,A.S., Watson,D.J. Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function. Neuroscience 2007; 148(-2-):359-370. >>> NT-4/5, recomb. human; PBS; BSA; CSF/CNS (somatosensory cortex); 1007D; ; Controls received mp w/ vehicle; tissue perfusion (cortex); good methods (p. 360); ALZET brain infusion kit 2 used; brain tissue distribution; post op. care (recovery on heating pad); animal info (male, Sprague-Dawley, 350-400g); 0.1% BSA; traumatic brain injury.

P8389 Jabbar,S., Harada,F., Aita,M., Ohishi,M., Saito,I., Kawano,Y., Suzuki,A., Nozawa-Inoue,K., Maeda,T. Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage. Journal of Comparative Neurology 2007; 501(-3-):400-412. >>> NT-4/5, recomb.; Saline, physiological; SC; Mice; 1002; 7, 14 days; Controls received mp w/ vehicle, or no treatment; replacement therapy (nt-4-5 knock out); peptides; animal info (12 wks old, 20-28g.); inferior alveolar nerve transection.

P7978 Engelhardt,M., Di Cristo,G., Berardi,N., Maffei,L., Wahle,P. Differential effects of NT-4, NGF and BDNF on development of neurochemical architecture and cell size regulation in rat visual cortex during the critical period. European Journal of Neuroscience 2007; 25(-2-):529-540. >>> Nerve growth factor, mouse; NT-4, human recomb.; brain-derived neurotrophic factor, recomb. human; CSF/CNS (visual cortex); Rat; 1007D; 8 days; Post op. care (antibiotics and local anesthetics); animal info (Long-Evans); dental cement used; 30 G cannula used; tissue perfusion (visual cortex).

P6930 Gillespie,L.N., Clark,G.M., Marzella,P.L. Delayed neurotrophin treatment supports auditory neuron survival in deaf guinea pigs. NeuroReport 2004; 15(-7-):1121-1125. >>> Brain-derived neurotrophic factor; nerve growth factor; NT-3; NT-4/5; Ear (scala tympani, cochlea); Guinea-pig; 2004; 28 days; Tissue perfusion.

P6328 Cabelli,R.J., Shelton,D.L., Segal,R.A., Shatz,C.J. Blockade of Endogenous Ligands of TrkB Inhibits Formation of Ocular Dominance Columns. Neuron 1997; 19(--):63-76. >>> Immunoglobulin G, TrkA; immunoglobulin, TrkB; immunoglobulin G, TrkC; NT-4/5; NT-3; BSA; PBS, sterile; CSF/CNS (visual cortex); Cat; ferret; 2002; 2,3 weeks; 2 days; Controls received mp w/ vehicle, or IgG protein fusions unrelated to Trk; comparison of CSF/CNS injections vs. mp; stability verified by Ab immunostaining of brain sections; peptides; post op. care (7 days oral amoxicillin); 3 week infusions had a flow rate of approx. 0.33 ul/hr, "the reduced flow rate (~0.33 ul/hr) was achieved by coating ~1/3 of the surface area of the minipump with hot paraffin prior to filling") p. 74).

P6243 Viegi,A., Cotrufo,T., Berardi,N., Mascia,L., Maffei,L. Effects of dark rearing on phosphorylation of neurotrophin Trk receptors. European Journal of Neuroscience 2002; 16(-10-):1925-1930. >>> Nerve growth factor; brain-derived neurotrophic factor, recomb. human; NT-4; CSF/CNS (visual cortex); Rat; 8 days; Controls received mp w/ cytochrome c; peptides.

P5632 Wahle,P., Di Cristo,G., Schwerdtfeger,G., Engelhardt,M., Berardi,N., Maffei,L. Differential effects of cortical neurotrophic factors on development of lateral geniculate nucleus and superior colliculus neurons: anterograde and retrograde actions. Development 2003; 130(-3-):611-622. >>> Nerve growth factor; NT-4/5; brainderived neurotrophic factor; leukemia inhibitory factor; CSF/CNS (visual cortex); Rat; 1007D; 7 days; Controls received mp w/ cytochrome C; peptides..

P5537 Lodovichi,C., Berardi,N., Pizzorusso,T., Maffei,L. Effects of neurotrophins on cortical plasticity: same or different?. J.Neurosci. 2000/3/15; 20(-6-):2155-2165. >>> Nerve growth factor; NT-3; NT-4; brain-derived neurotrophic factor; cytochrome C; CSF/CNS (visual cortex); Rat; 1007D; 1 week; Functionality of mp verified by detection of neurotrophins by immunohistochemistry; peptides; brain tissue distribution; mouse NGF, human recomb. NT-3 AND NT-4, and human recomb. BDNF used.

P5232 Hagg,T. Neurotrophins prevent death and differentially affect tyrosine hydroxylase of adult rat nigrostriatal neurons in vivo. Exp.Neurol. 1998/1; 149(-1-):183-192. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-3; NT-4; PBS; Albumin, rat serum; Buffer, sodium acetate; CSF/CNS (nigrostriatal complex); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides: stability (p.184); "at 1 mg/ml these factors retain more than 70% of their activity after 14 days at 37 C in ALZET pumps"; pumps partially coated with paraffin to reduce flow rate to 0.25 ul/hr; lynch coil used; neuronal survival; neurodegenerative (Parkinson's disease).

P4747 Kernie,S.G, Liebl,D.J, Parada,L.F. BDNF regulates eating behavior and locomotor activity in mice.. The European Molecular Biology Organization Journal 2000; 19(-6-):1290-1300. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-4/5; PBS; CSF/CNS (third ventricle); Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume after sacrifice; peptides; ALZET brain infusion kit used; brain cannula secured to skull by cyanoacrylate adhesive (Loctite 454); cannula patency verified by methlene blue injection after sacrifice; recomb. human BDNF used; all neurotrophins infused via same pump;.

P4714 Gillespie,D.C, Crair,M.C, Stryker,M.P. Neurotrophin-4/5 alters responses and blocks the effect of monocular deprivation in cat visual cortex during the critical period.. The Journal of Neuroscience 2000; 20(-24-):9174-9186. >>> NT-4/5; NT-3; Nerve growth factor;; PBS; BSA; Sodium acetate;; CSF/CNS (visual cortex);; cat; cat (kitten);; 1007D; 2001;; 7 days;; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 9175) some cannulae were found to be obstructed at the end of experiment; peptides; cannulae patency verified at the end of experiment;.

P4391 Kobayashi,N.R., Fan,D.-P., Giehl,K.M., Bedard,A.M., Wiegand,S.J., Tetzlaff,W. BDNF and NT-4/5 prevent atrophy of rat rubrospinal neurons after cervical axotomy, stimulate GAP-43 and Ta1-tubulin mRNA expression, and promote axonal regeneration. Journal of Neuroscience 1997/12/15; 17(-24-):9583-9595. >>> Nerve growth factor; Brain-derived neurotrophic factor; NT-3; NT-4/5;; PBS, sterile; Albumin, rat serum; Penicillin; Streptomycin;; CSF/CNS (red nucleus);; rat;; 2001;; 7 days;; controls received mp with vehicle or no pump; tissue perfusion (rubrospinal neurons); peptides; brain tissue distribution of neurotrophins assessed by immunohistochemistry (p. 9587); schematic of model, p. 9587; recomb. human NGF, BDNF, NT-4/5, and NT-3 used;.

P4385 Fernandes,K.J.L., Kobayashi,N.R., Jasmin,B.J., Tetzlaff,W. Acetylcholinesterase gene expression in axotomized rat facial motoneurons is differentially regulated by neurotrophins: Correlation with trkB and trkC mRNA levels and isoforms. Journal of Neuroscience 1998/12/1; 18(-23-):9936-9947. >>> Nerve growth factor; NT-3; NT-4/5; Brain-derived neurotrophic factor;; Saline; Buffer, sodium phosphate; Albumin, rat serum; Penicillin; Streptomycin;; CSF/CNS (facial nerve);; rat;; 2001;; 7 days;; controls received mp with vehicle; tissue perfusion (nerve stump); dose-response for BDNF (p. 9939); peptides; some animals underwent bilateral axotomy, with one pump infusing each side; silastic tubing was sutured in place over nerve stump, and adhesive prevented leakage from tubing;.

P4264 Mendell,L.M., Johnson,R.D., Munson,J.B. Neurotrophin modulation of the monosynaptic reflex after peripheral nerve transection. Journal of Neuroscience 1999/4/15; 19(-8-):3162-3170. >>> NT-3; NT-4/5; Mannitol; Sucrose; Histidine;; CSF/CNS (gastocnemius nerve); cat; 2ML2; 2ML4;; 8-35 days; axotomized nerve controls received mp w/ vehicle or SC NT-3; tissue perfusion (medial gastrocnemius nerve); good methods (p. 3163); peptides; schematic of delivery model (p. 3163); 10 mm Gore-tex sleeve on nerve was connected via silastic tubing to pump.

P4259 Clarke,D.B., Bray,G.M., Aguayo,A.J. Prolonged administration of NT-4/5 fails to rescue most axotomized retinal ganglion cells in adult rats. Vision Research 1998; 38(--):1517-1524. >>> NT-4/5; PBS; Eye (vitreous chamber); rat; 2002; 14,28 days; controls received mp w/vehicle; comparison of injections vs. mp; peptides.

P3667 Munson,J.B., Shelton,D.L., McMahon,S.B. Adult mammalian sensory and motor neurons: roles of endogenous neurotrophins and rescue by exogenous neurotrophins after axotomy. Journal of Neuroscience 1997/1/1; 17(-1-):470-476. >>> Fusion protein; NT-3; NT-4/5;; Acetate; Saline;; CSF/CNS (gastrocnemius nerve); CSF/CNS (fibial nerve);; rat;; 2002;; 2 weeks;; controls received mp with vehicle; tissue perfusion (nerve stump;) tissue perfusion (intact nerve); peptides; schematic of delivery model (p. 471);.

P3483 Sawai,H., Clarke,D.B., Kittlerova,P., Bray,G.M., Aguayo,A.J. Brain-derived neurotrophic factor and neurotrophin-4/5 stimulate growth of axonal branches from regenerating retinal ganglion cells. J.Neurosci. 1996; 16(-12-):3887-3894. >>> NT-4/5; PBS; Eye (vitreous chamber); rat; 2002; 14 days; comparison of intraocular injections vs. mp.

P3413 Cabelli,R.J., Hohn,A., Shatz,C.J. Inhibition of ocular dominance column formation by infusion of NT-4/5 or BDNF. Science 1995; 267(--):1662-1666. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-4/5; NT-3; CSF/CNS (primary visual cortex); cat; 8,14,15,16 days; peptides.

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6. Nerve Growth Factor

16152 Pereira, P. A., Rocha, J. P., Cardoso, A., Vilela, M., Sousa, S., Madeira, M. D. Effects of chronic alcohol consumption, withdrawal and nerve growth factor on neuropeptide Y expression and cholinergic innervation of the rat dentate hilus. Neurotoxicology 2016; 54(--):153-60. >>> Nerve growth factor; Methylene blue; BSA; CSF, artificial; CSF/CNS; Rat; 2002; animal info (male, Wistar); functionality of mp verified by residual volume; ALZET brain infusion kit used; post op. care (SC injections of 0.9% saline (2ml)); pulsed delivery; used PE-60 tubing; used lynch coil;.

15856 Yokomizo, A., Takatori, S., Hashikawa-Hobara, N., Goda, M., Kawasaki, H. Nerve growth factor facilitates redistribution of adrenergic and non-adrenergic non-cholinergic perivascular nerves injured by phenol in rat mesenteric resistance arteries. Eur J Pharmacol 2016; 770(--):110-6. >>> Nerve growth factor; Saline, sterile; IP; Rat; 1007D; animal info (Wistar, 8 weeks old); Dose (20 ug/kg/day);.

15757 Ciro De Luca, Leonilde Savarese, Anna Maria Colangelo, Maria Rosaria Bianco, Giovanni Cirillo, Lilia Alberghina, Michele Papa. Astrocytes and Microglia-Mediated Immune Response in Maladaptive Plasticity is Differently Modulated by NGF in the Ventral Horn of the Spinal Cord Following Peripheral Nerve Injury. Cell Mol Neurobiol 2016; (-36-):37-46. >>> Nerve growth factor, b-; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-300g); Cannula placement verified via induced lower body paralysis (IT lidocane injection); spared nerve injury; Dose (12 ug/kg/day);.

15231 Schlachetzki, J.C.M., Pizzo, D.P., Morrissette, D.A., Winkler, J. Intracerebroventricular Administration of Nerve Growth Factor Induces Gliogenesis in Sensory Ganglia, Dorsal Root, and within the Dorsal Root Entry Zone. BIOMED RESEARCH INTERNATIONAL 2014; ;(-;-):U1-U9. >>> Nerve growth factor, human recombinant; Cytochrome C; CSF, artificial; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle; animal info (male, Fischer 344 albino, 281.8 +/- 2g); used Plastics One cannula;.

14883 Chen, W.H., Mao, C.Q., Zhuo, L.L., Ong, J.L., Chen, W.H. Beta-nerve growth factor promotes neurogenesis and angiogenesis during the repair of bone defects. Neural Regeneration Research 2015; 10(--):1159-1165. >>> Nerve growth factor, b-; PBS; Evans blue dye; Bone (parietal); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Sprague Dalwey, 250-300g); 1% Evans blue used; good methods (picture of dual cannula impmlantation pg 1161); Multiple pumps per animal (2); pump with agent inserted into right pocket, pump with PBS inserted into left pocket; bilateral infusion;.

14261 Lee, J.Y., Jahng, J.W., Kim, S.M., Kim, M.J., Lee, J.H., Lee, J.H. Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study. Journal of Oral and Maxillofacial Surgery 2015; 73(--):410-423. >>> Nerve growth factor, human B-; PBS; CSF/CNS (inferior alveolar nerve); Dog (beagle); 2ML2; Controls received mp w/ vehicle; animal info (male, beagle, 18 weeks old, 10-12 kg); good methods (picture of implant pg 413); Multiple pumps per animal (2; one pump delivered NGF other delivered PBS); used rat jugular catheter, 15 cm long; pump body placed into retromandibular area; long-term study;.

14605 Pereira, P.A., Neves, J., Vilela, M., Sousa, S., Cruz, C., Madeira, M.D., Pereira, P.A. Chronic alcohol consumption leads to neurochemical changes in the nucleus accumbens that are not fully reversed by withdrawal. NEUROTOXICOLOGY AND TERATOLOGY 2014; 44(--):53-61. >>> Nerve growth factor; CSF, artificial; methylene blue; CSF/CNS; Rat; 2002; Control animals received mp w/ vehicle; animal info (male, Wistar); Lynch coil; PE60 tubing used; ALZET brain infusion kit used;"The cannulae were connected to methylene blue... filled Alzet osmotic minipumps... via sterile coiled polyethylene tubing... This tubing was filled with air-oil spacer at the pump end and with NGF."pg 54; pump functionality verified via residual volume.

14598 Shaqura, M., Khalefa, B.I., Shakibaei, M., Zoellner, C., Al-Khrasani, M., Fuerst, S., Schaefer, M., Mousa, S.A., Mousa, S.A. New insights into mechanisms of opioid inhibitory effects on capsaicin-induced TRPV1 activity during painful diabetic neuropathy. Neuropharmacology 2014; 85(--):142-150. >>> Nerve growth factor, beta; albumin, rat serum; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; Control animals received mp w/ animal info (male, Wistar, STZ induced diabetes).

13950 Yegla, B., Parikh, V. EFFECTS OF SUSTAINED PRONGF BLOCKADE ON ATTENTIONAL CAPACITIES IN AGED RATS WITH COMPROMISED CHOLINERGIC SYSTEM. Neuroscience 2014; 261(-;-):118-132. >>> Antibody, pro-nerve growth factor; PBS; CSF/CNS; Rat; 1004; Controls received mp w/ vehicle; animal info (male, Wistar, 23 months old); ALZET brain infusion kit used; good methods (cannula placement pg.124, fig.2); post op. care (triple antibiotic cream on wound; Baytril injected; buprenorphine); behavioral testing (sustained attention task, performance measures); Cannula placement verified via Nissl staining; used dental cement and bone screws; pumps and cannula removed after 4 weeks;.

13507 Tomioka, T., Shimazaki, T., Yamauchi, T., Oki, T., Ohgoh, M., Okano, H. LIM Homeobox 8 (Lhx8) Is a Key Regulator of the Cholinergic Neuronal Function via a Tropomyosin Receptor Kinase A (TrkA)-mediated Positive Feedback Loop. Journal of Biological Chemistry 2014; 289(-2-):1000-1010. >>> Nerve growth factor, murine; PBS; CSF/CNS; Rat; 2001; Control animals received mp w/ vehicle; animal info (adult, male, Sprague Dawley, 8 wks old); ALZET brain infusion kit 2 used.

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What researchers are saying ...

"Utilizing continuous minipump administration enhances the knockdown to 63% of control." (p. 619) This compared with 40% by twice daily administration. "The results of the present study confirm the utility of osmotic minipump administration of antisense oligonucleotides." (p. 621) Falk et al., Pharmacol Biochem Behav 2002;72:617-622.