References on the Administration of Neurotrophic Factors
Using ALZET^® Osmotic Pumps

1. Brain-derived Neurotrophic Factor

Q0837 Warner-Schmidt,J.L., Chen,E.Y., Zhang,X.Q., Marshall,J.J., Morozov,A., Svenningsson,P., Greengard,P. A Role for p11 in the Antidepressant Action of Brain-Derived Neurotrophic Factor. Biological Psychiatry 2010; 68(-6-):528-535. >>> Brain-derived neurotrophic factor, recomb.; CSF/CNS; Mice (transgenic); 1003D; 3 days; Animal info (8-10 wks old, C57BL/6, BDNF Tg); behavioral testing (tail suspension test, forced swim test, open field locomotor activity); Plastics One cannula used.

Q0828 Wu,S.Y., Wang,T.F., Yu,L., Jen,C.J., Chuang,J.I., Wu,F.S., Wu,C.W., Kuo,Y.M. Running exercise protects the substantia nigra dopaminergic neurons against inflammation-induced degeneration via the activation of BDNF signaling pathway. BRAIN BEHAVIOR AND IMMUNITY 2011; 25(-1-):135-146. >>> Brain-derived neurotrophic factor, recomb. human; CSF, artificial; CSF/CNS (striatum); Mice; 1002; 14 days; Controls received sham surgery; animal info (C57BL/6J, 8 wks old, 20-25 g).

Q0693 Rohe,M., Synowitz,M., Glass,R., Paul,S.M., Nykjaer,A., Willnow,T.E. Brain-Derived Neurotrophic Factor Reduces Amyloidogenic Processing through Control of SORLA Gene Expression. Journal of Neuroscience 2009; 29(-49-):15472-15478. >>> Brain-derived neurotrophic factor; CSF, artificial; CSF/CNS (hippocampus); Mice; 1007D; 7 days; Controls received mp w/ vehicle; animal info (BNDF -/-); ALZET brain infusion kit 2 used; neurodegenerative (Alzheimer's disease, Huntington's disease).

Q0588 Keiner,S., Witte,O.W., Redecker,C. Immunocytochemical Detection of Newly Generated Neurons in the Perilesional Area of Cortical Infarcts After Intraventricular Application of Brain-Derived Neurotrophic Factor. Journal of Neuropathology and Experimental Neurology 2009; 68(-1-):83-93. >>> Brain-derived neurotrophic factor; NaCl; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (male, Wistar, 12-14 weeks, 250-300 g); functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; ischemia (focal cortical infarct); behavioral testing (the limb-use asymmetry; tapered beam walking; ladder rung walking); fig. 1c diagram of a parasagittal section of the rat brain showing cannula placement.

Q0453 Agterberg,M.J.H., Versnel,H., van Dijk,L.M., de Groot,J.C.M.J., Klis,S.F.L. Enhanced Survival of Spiral Ganglion Cells After Cessation of Treatment with Brain-Derived Neurotrophic Factor in Deafened Guinea Pigs. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY 2009; 10(-3-):355-367. >>> Brain-derived neurotrophic factor; BSA; Ear (cochlea); Guinea pig; 2004; 4 weeks; Controls were untreated; animal info (albino, female, 250-350 g); pump connected to Cochlear (R) electrode array; tissue perfusion (cochlea).

Q0409 Schmidt,H.D., Duman,R.S. Peripheral BDNF Produces Antidepressant-Like Effects in Cellular and Behavioral Models. Neuropsychopharmacology 2010; 35(-12-):2378-2391. >>> Brain-derived neurotrophic factor, recomb; PBS; BSA; Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (C57BL/6, BALB/c, 8-10 wks old); behavioral testing (forced swim test, novelty induced hypophagia, chronic unpredictable stress).

Q0241 Im,S.H., Yu,J.H., Park,E.S., Lee,J.E., Kim,H.O., Park,K.I., Kim,G.W., Park,C.I., Cho,S.R. INDUCTION OF STRIATAL NEUROGENESIS ENHANCES FUNCTIONAL RECOVERY IN AN ADULT ANIMAL MODEL OF NEONATAL HYPOXIC-ISCHEMIC BRAIN INJURY. Neuroscience 2010; 169(-1-):259-268. >>> Brain-derived neurotrophic factor; epidermal growth factor; ara-C; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ICR, 6 wks old); behavioral testing (rotarod performance, forelimb-use asymmetry test).

Q0074 Geremia,N.M., Pettersson,L.M.E., Hasmatali,J.C., Hryciw,T., Danielsen,N., Schreyer,D.J., Verge,V.M.K. Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons. Experimental Neurology 2010; 223(-1-):128-142. >>> Brain-derived neurotrophic factor, anti-, sheep; immunoglobulin G, sheep; CSF/CNS (intrathecal); Rat; 2001; 3 days; Controls received mp w/ control IgG; post op. care (buprenorphine); animal info (male, Wistar, adult, 300-500 g.); sciatic nerve transection.

P9764 Nagahara,A.H., Merrill,D.A., Coppola,G., Tsukada,S., Schroeder,B.E., Shaked,G.M., Wang,L., Blesch,A., Kim,A., Conner,J.M., Rockenstein,E., Chao,M.V., Koo,E.H., Geschwind,D., Masliah,E., Chiba,A.A., Tuszynski,M.H. Neuroprotective effects of brain-derived neurotrophic factor in rodent and primate models of Alzheimer's disease. Nature Medicine 2009; 15(-3-):331-337. >>> Brain-derived neurotrophic factor, recomb. human; CSF, artificial; CSF/CNS (entorhinal cortex); Rat; 2004; 4 weeks; Controls received mp w/vehicle; animal info (cognitively impaired, Fischer); neurodegenerative (Alzheimer's disease); behavioral testing (morris water maze test); bilateral cannula used; brain tissue distribution.

P9656 Snapyan,M., Lemasson,M., Brill,M.S., Blais,M., Massouh,M., Ninkovic,J., Gravel,C., Berthod,F., Goetz,M., Barker,P.A., Parent,A., Saghatelyan,A. Vasculature Guides Migrating Neuronal Precursors in the Adult Mammalian Forebrain via Brain-Derived Neurotrophic Factor Signaling. Journal of Neuroscience 2009; 29(-13-):4172-4188. >>> Brain-derived neurotrophic factor; immunoglobulin-G-Fe; RNA, small interfering; TrkB-Fr; NaCI; IA (carotid); CSF/CNS (rostral migratory, system); Mice; 1007D; 7 days; Controls received mp w/control siRNA; animal info (2-3 mo old C57BL/J6); brain tissue distribution; ALZET mouse jugular catheter used; heparin added to BDNF.

P9516 Wissman,A.M., Brenowitz,E.A. The Role of Neurotrophins in the Seasonal-Like Growth of the Avian Song Control System. Journal of Neuroscience 2009; 29(-20-):6461-6471. >>> TrkB-Fc; TrkC-Fc; brain-derived neurotrophic factor; nerve growth factor; PBS; CSF/CNS (robust nucleus of the arcopallium); Bird; 1002; ; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; animal info (sparrow, adult, male, Gambel's white crowned).

P9447 Song,X.Y., Li,F., Zhang,F.H., Zhong,J.H., Zhou,X.F. Peripherally-Derived BDNF Promotes Regeneration of Ascending Sensory Neurons after Spinal Cord Injury. PLoS One 2008; 3(-3-):U22-U37. >>> Brain-derived neurotrophic factor, recomb. human; CSF/CNS (sciatic nerve); Rat; 1007D; 7 days; Controls received mp w/ PBS; comparison of foot pad injections vs. mp; peptides; animal info (female, Sprague Dawley, 8-10 wks old, SCI); behavioral testing (hind limb motor function).

P9341 Callaway,C.W., Ramos,R., Logue,E.S., Betz,A.E., Wheeler,M., Repine,M.J. Brain-derived neurotrophic factor does not improve recovery after cardiac arrest in rats. Neuroscience Letters 2008; 445(-1-):103-107. >>> Brain-derived neurotrophic factor; CSF/CNS; Rat; 1003D; ; Controls received mp w/ saline; saline filled pumps replaced after cardiac arrest w/ BDNF filled pumps; ischemia (cerebral); PE-50 tubing used; bilateral infusion via 25 g injectors.

P9237 Galvao,R.P., Garcia-Verdugo,J.M., varez-Buylla,A. Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats. Journal of Neuroscience 2008; 28(-50-):13368-13383. >>> Ara-C; brain-derived neurotrophic factor; Saline; BSA; CSF/CNS; Rat; mice; 1007D; 2002; 6, 14 days; Controls received mp w/ vehicle; functionality of mp verified by weight loss due to BDNF's appetite suppression (p. 13377), residual volume; stability verified by testing activity of residual BDNF volume after 14 days/37 degrees Celsius; brain tissue distribution; peptides; post op. care (Buprenorphine); animal info (male, Sprague Dawley, 250 g., male CD-1 3 mo old); mp/cannula placement visually confirmed after brain sectioning.

P9224 Reyes,J.H., O'Shea,K.S., Wys,N.L., Velkey,J.M., Prieskorn,D.M., Wesolowski,K., Miller,J.M., Altschuler,R.A. Glutamatergic Neuronal Differentiation of Mouse Embryonic Stem Cells after Transient Expression of Neurogenin 1 and Treatment with BDNF and GDNF: In Vitro and In Vivo Studies. Journal of Neuroscience 2008; 28(-48-):12622-12631. >>> Doxycycline; brain-derived neurotrophic factor; glial cell line-derived neurotrophic factor; Ear (scala tympani); Guinea-pig; 2002; 27 days; Controls received no treatment to contralateral ear; pumps replaced; peptides; tissue perfusion (scala tympani); animal info (NIH strain, 275-315 g., deafened); cannula and catheter contained doxycycline, mp contained BDNF/GDNF (delayed delivery) to follow, thus providing 2 days Dox, 25 days BDNF/GDNF.

P9218 Shepherd,R.K., Coco,A., Epp,S.B. Neurotrophins and electrical stimulation for protection and repair of spiral ganglion neurons following sensorineural hearing loss. Hearing Research 2008; 242(-1-2-):100-109. >>> Brain-derived neurotrophic factor, recomb. human; Ringer's solution; albumin, guinea pig; Ear (scala tympani); Guinea-pig; 2004; 4 weeks; Controls received no treatment to contralateral cochlea; functionality of mp verified by residual volume and intact connections; peptides; post op. care (Carprofen, Baytril); tissue perfusion (scala tympani); animal info (pigmented, 400-844 g., kanamycin/furosemide deafened).

P9167 Bretzner,F., Liu,J., Currie,E., Roskams,A.J., Tetzlaff,W. Undesired effects of a combinatorial treatment for spinal cord injury - transplantation of olfactory ensheathing cells and BDNF infusion to the red nucleus. European Journal of Neuroscience 2008; 28(-9-):1795-1807. >>> Brain-derived neurotrophic factor; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 300-400 g.); 28 gauge cannula, silastic tubing used; spinal cord injury; wound clips used.

P9051 Song,B.N., Li,Y.X., Han,D.M. Effects of delayed brain-derived neurotrophic factor application on cochlear pathology and auditory physiology in rats. Chinese Medical Journal 2008; 121(-13-):1189-1196. >>> Brain-derived neurotrophic factor, recomb. human; Albumin, rat; Ringer's solution; Ear (cochlea); Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, albino, Sprague Dawley, 10-12 wks old, 220-250 g.); tissue perfusion (cochlea); functionality of mp verified by residual volume; good methods pg. 1191; pumps were primed in sterile saline at 37 degree Celsius for 48 hours; 1-cm len.

P9013 Ruggieri,MR Sr, Braverman,A.S., D'Andrea,L., Simpkiss,B., Kozin,S.H., Pontari,M.A., Betz,R., Barbe,M.F. Functional reinnervation of the canine bladder after spinal root transection and immediate end-on-end repair. Journal of Neurotrauma 2006; 23(-7-):1125-1136. >>> Brain-derived neurotrophic factor; CSF/CNS (spinal cord, intrathecal); Dog; 2002; 14 days; Controls received mp w/ saline; dose-response; peptides; post io. care (ketoprofen); animal info (female, mongrel hound, 6-12 months old, 18-22 kg., bladder denervation); mp connected to silicone cuff surrounding spinal cord root bundles (2).

P8932 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; 3 days; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.

P8878 Hontanilla,B., Auba,C., Gorria,O. Nerve regeneration through nerve autografts after local administration of brain-derived neurotrophic factor with osmotic pumps. Neurosurgery 2007; 61(-6-):1268-1274. >>> Brain-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Rat; 1002; 2 weeks; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve graft); replacement therapy (axotomy); no stress (see pg. 1270); stress/adverse reaction: (see pg. 1270) "one pump was extruded"; animal info (male, Wistar, 200-250g.); pg. 1269, fig A shows image of pump implantation and cannulae location.

P8803 Pinnock,S.B., Herbert,J. Brain-derived neurotropic factor and neurogenesis in the adult rat dentate gyrus: interactions with corticosterone. European Journal of Neuroscience 2008; 27(-10-):2493-2500. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, rat serum; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 2496) 1 animal died (cause unknown); peptides; animal info (male, Lister-Hooded, 200-250g.); cannula position confirmed post mortem with cresyl violet.

P8690 Vetencourt,J.F.M., Sale,A., Viegi,A., Baroncelli,L., De Pasquale,R., O'Leary,O.F., Castren,E., Maffei,L. The antidepressant fluoxetine restores plasticity in the adult visual cortex. Science 2008; 320(-5874-):385-388. >>> Brain-derived neurotrophic factor; CSF/CNS (intracortical); Rat; 1 week.

P8661 Miller,J.M., Le Prell,C.G., Prieskorn,D.M., Wys,N.L., Altschuler,R.A. Delayed neurotrophin treatment following deafness rescues spiral ganglion cells from death and promotes regrowth of auditory nerve peripheral processes: Effects of brain-derived neurotrophic factor and fibroblast growth factor. Journal of Neuroscience Research 2007; 85(-9-):1959-1969. >>> Brain-derived neurotrophic factor; fibroblast growth factor-1; Perilymph, artificial; albumin, guinea pig serum; Ear (scala tympani); Guinea-pig; 2002; 26 days; Controls received mp w/ vehicle; pumps replaced at day 13; peptides; tissue perfusion (scala tympani); animal info (male, female, pigmented, 250-300g, deafened).

P8587 Mamounas,L.A., Altar,C.A., Blue,M.E., Kaplan,D.R., Tessarollo,L., Lyons,W.E. BDNF promotes the regenerative sprouting, but not survival, of injured serotonergic axons in the adult rat brain. J Neurosci 2000; 20(-2-):771-782. >>> Brain-derived neurotrophic factor, recomb. human; PBS, sterile; CSF/CNS (frontoparietal cortex); CSF/CNS (dorsal hippocampus); Rat; 1007D; 2002; 2004; 1,2,3 weeks; 1,3,8,18 days; Controls received mp w/ vehicle; dose-response (fig. 4); peptides; brain tissue distribution; animal info (male, Sprague-Dawley, 225-250 grams); mp and cannula primed 4-18 hours in 37 C PBS.

P8176 Radeloff,A., Smolders,J.W.T. Brain-derived neurotrophic factor treatment does not improve functional recovery after hair cell regeneration in the pigeon. Acta Oto-laryngologica 2006; 126(-5-):452-459. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, chicken; Hank's solution; Ear (scala tympani); Bird (pigeon); 2002; 8 weeks; Controls received mp w/ vehicle or no treatment to contralateral ear; long-term study; pumps replaced every 14 days; ALZET brain infusion kit used; peptides; animal info (Columba livia, 6 months old, 320-580 grams); deafening of both ears; tissue perfusion (scala tympani).

P8012 Takahashi,M., Kakita,A., Futamura,T., Watanabe,Y., Mizuno,M., Sakimura,K., Castren,E., Nabeshima,T., Someya,T., Nawa,H. Sustained brain-derived neurotrophic factor up-regulation and sensorimotor gating abnormality induced by postnatal exposure to phencyclidine: comparison with adult treatment. Journal of Neurochemistry 2006; 99(-3-):770-780. >>> Brain-derived neurotrophic factor; SC; Rat; 2002; 10 days; Controls received mp w/ saline; animal info (male, 8 weeks old, Wistar).

P7994 Vavrek,R., Girgis,J., Tetzlaff,W., Hebert,G.W., Fouad,K. BDNF promotes connections of corticospinal neurons onto spared descending interneurons in spinal cord injured rats. Brain 2006; 129(--):1534-1545. >>> Brain-derived neurotrophic factor; NT-3; Albumin, rat serum; CSF/CNS (left motor cortex, intrathecal, cisterna magna); Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; SCI (spinal cord injury); dental cement used to secure cannula; NT-3 delivered intrathecally; tissue perfusion (left motor cortex).

P7978 Engelhardt,M., Di Cristo,G., Berardi,N., Maffei,L., Wahle,P. Differential effects of NT-4, NGF and BDNF on development of neurochemical architecture and cell size regulation in rat visual cortex during the critical period. European Journal of Neuroscience 2007; 25(-2-):529-540. >>> Nerve growth factor, mouse; NT-4, human recomb.; brain-derived neurotrophic factor, recomb. human; CSF/CNS (visual cortex); Rat; 1007D; 8 days; Post op. care (antibiotics and local anesthetics); animal info (Long-Evans); dental cement used; 30 G cannula used; tissue perfusion (visual cortex).

P7874 Naert,G., Ixart,G., Tapia-Arancibia,L., Givalois,L. Continuous i.c.v. infusion of brain-derived neurotrophic factor modifies hypothalamic-pituitary-adrenal axis activity, locomotor activity and body temperature rhythms in adult male rats. Neuroscience 2006; 139(-2-):779-789. >>> Brain-derived neurotrophic factor; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle, or no treatment; comparison of acute icv injections vs. mp; no stress (see pg. 782); peptides; animal info (male, Sprague-Dawley, 375g.); mp + tubing primed overnight in 37 celsius saline; telemetric transmitter IP.

P7870 Nakai,S., Matsunaga,W., Ishida,Y., Isobe,K., Shirokawa,T. Effects of BDNF infusion on the axon terminals of locus coeruleus neurons of aging rats. NEUROSCIENCE RESEARCH 2006; 54(-3-):213-219. >>> Brain-derived neurotrophic factor; antibody, anti-BDNF; brain-derived neurotrophic factor, biotin-labeled; immunoglobulin G; PBS; BSA; CSF/CNS (frontal cortex); Rat; 2002; 14 days; Controls received mp w/ vehicle, or IgG; dose-response (fig. 3); ALZET brain infusion kit used; brain tissue distribution; peptides; post op. care (enrofloxacin); animal info (male, F344/N, 13 month old, 19 month old); mp primed 3-6 hours in 37 celsius saline.

P7816 Givalois,L., Naert,G., Tapia-Arancibia,L., Arancibia,S. Involvement of brain-derived neurotrophic factor in the regulation of hypothalamic somatostatin in vivo. Journal of Endocrinology 2006; 188(-3-):425-433. >>> Brain-derived neurotrophic factor, recomb. human; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle, or no treatment; comparison of icv injections vs. mp; peptides; animal info (male, Sprague-Dawley, 260-280g.); mp primed overnight in 37 celsius saline.

P7765 Richardson,R.T., O'Leary,S., Wise,A., Hardman,J., Clark,G. A single dose of neurotrophin-3 to the cochlea surrounds spiral ganglion neurons and provides trophic support. Hearing Research 2005; 204(-1-2-):37-47. >>> NT-3, recomb. human; brain-derived neurotrophic factor; Ringer's solution; Ear (scalatympani); Guinea-pig; 2004; 28 days; Comparison of bolus infusion vs. mp; peptides; animal info (adult, pigmented Dunkin-Hartley, 455g.); tissue perfusion (scala tympani).

P7592 Imamura,K., Morii,H., Nakadate,K., Yamada,T., Mataga,N., Watanabe,Y., Mori,N. Brain-derived neurotrophic factor enhances expression of superior cervical ganglia clone 10 in lateral geniculate nucleus and visual cortex of developing kittens. European Journal of Neuroscience 2006; 23(-3-):637-648. >>> Brain-derived neurotrophic factor, recomb. human; PBS; BSA; CSF/CNS (visual cortex); Cat (kitten); cat; 2002; 14 days; Controls received mp w/ vehicle; brain tissue distribution; peptides; multiple pumps per animal (2); post op. care (ampicillin sodium).

P7494 Bariohay,B., Lebrun,B., Moyse,E., Jean,A. Brain-derived neurotrophic factor plays a role as an anorexigenic factor in the dorsal vagal complex. Endocrinology 2005; 146(-12-):5612-5620. >>> Brain-derived neurotrophic factor, recomb. human; CSF, artificial; CSF/CNS (dorsal vagal complex); Rat; 1002; 15 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (fig. 2); brain tissue distribution; peptides; animal info (male, Wistar, 290 g); cannula placement verified with pontamine blue dye.

P7470 Plantman,S., Novikova,L., Novikov,L., Hammarberg,H., Wallquist,W., Kellerth,J.O., Cullheim,S. Integrin messenger RNAs in the red nucleus after axotomy and neurotrophic administration. NeuroReport 2005; 16(-7-):709-713. >>> Brain-derived neurotrophic factor, recomb human; NT-3; CSF/CNS (intrathecal, subarachnoid space); Rat; 1, 3 weeks; Controls recieved no treatment; peptides; animal info (female, Sprague-Dawley); spinal hemisection (peripheral axotomy).

P7368 Mohapel,P., Frielingsdorf,H., Haeggblad,J., Zachrisson,O., Brundin,P. Platelet-derived growth factor (PDGF-BB) and brainderived neurotrophic factor (BDNF) induce striatal neurogenesis in adult rats with 6-hydroxydopamine lesions. Neuroscience 2005; 132(-3-):767-776. >>> Platelet-derived growth factor-BB; brain-derived neurotrophic factor; uridine, bromodeoxy-; PBS; CSF/CNS; Rat; 2002; 10 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit used; neurodegenerative (parkinson's disease); labeling of striatum.

P7352 Scharfman,H., Goodman,J., Macleod,A., Phani,S., Antonelli,C., Croll,S. Increased neurogenesis and the ectopic granule cells after intrahippocampal BDNF infusion in adult rats. Experimental Neurology 2005; 192(-2-):348-356. >>> Brain-derived neurotrophic factor; PBS; BSA; CSF/CNS (dorsal hilus); Rat; 2002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by immunochemistry&residual volume; brain tissue distribution; plastics one cannula; neurogenesis.

P7331 Wise,A.K., Richardson,R., Hardman,J., Clark,G., O'Leary,S. Resprouting and survival of guinea pig cochlear neurons in response to the administration of the neurotrophins brain-derived neurotrophic factor and neurotrophin-3. Journal of Comparative Neurology 2005; 487(-2-):147-165. >>> Brain-derived neurotrophic factor, recomb. human; NT-3; Ear (scala tympani); Guinea-Pig; 2004; 28 days; Controls received mp w/ Ringer's solution; cannula patency ascertained by visual inspection; tissue perfusion (scala tympani).

P7306 Shepherd,R.K., Coco,A., Epp,S.B., Crook,J.M. Chronic depolarization enhances the trophic effects of brain-derived neurotrophic factor in rescuing auditory neurons following a sensorineural hearing loss. Journal of Comparative Neurology 2005; 486(-2-):145-158. >>> Brain-derived neurotrophic factor, recomb. human; perilymph, artificial; Ringer's solution; albumin, recomb. guinea-pig; Ear (scala tympani); Guinea-Pig; 2004; 28 days; Controls received mp w/ artificial perilymph or untreated contralateral cochlea; functionality of mp verified by residual fluid, cannula connection and patency; no stress (see pg. 155); good methods; peptides; post op. care (carprofen, Baytril); mp primed 36-48 hours in 37 degrees celsius Ringer's solution; Electrode array also inserted into scala tympani; "We observed no evidence of mechanical trauma to cochlea following long-term implantation and AP or BDNF delivery." (p. 155); tissue perfusion (scala tympani).

P7212 Radecki,D.T., Brown,L.M., Martinez,J., Teyler,T.J. BDNF protects against stress-induced impairments in spatial learning and memory and LTP. Hippocampus 2005; 15(-2-):246-253. >>> Brain-derived neurotrophic factor, recomb. human; CSF/CNS (hippocampus); Rat; 2002; 14 days; Controls received mp w/ vehicle, or PBS or no treatment; functionality of mp verified by residual volume; no stress (see pg. 248); brain tissue distribution; peptides; cyanoacrylate adhesive; "Direct hippocampal infusion was used in our studies to maximize the targeting of BDNF within the hippocampus." (p. 251) Memory study.

P7209 Wimmer,C., Mees,K., Stumpf,T., Welsch,U., Reichel,O., Suckfuell,M. Round window application of D-methionine, sodium thiosulfate, brain-derived neurotrophic factor, and fibroblast growth factor-2 in cisplatin-induced ototoxicity. OTOLOGY&NEUROTOLOGY 2004; 25(-1-):33-40. >>> Methionine, D-; filoblast growth factor 2; thiosulfate, sodium petahydrate; brain-derived neurotrophic factor; Water; PBS; Ear (round window membrane); Guinea-Pig; 2001; 6 days; Controls received mp w/ saline; mp primed > 12 hours in saline; tissue perfusion (round window membrane).

P6930 Gillespie,L.N., Clark,G.M., Marzella,P.L. Delayed neurotrophin treatment supports auditory neuron survival in deaf guinea pigs. NeuroReport 2004; 15(-7-):1121-1125. >>> Brain-derived neurotrophic factor; nerve growth factor; NT-3; NT-4/5; Ear (scala tympani, cochlea); Guinea-pig; 2004; 28 days; Tissue perfusion.

P6890 Liu,I.Y.C., Lyons,W.E., Mamounas,L.A., Thompson,R.F. Brain-derived neurotrophic factor plays a critical role in contextual fear conditioning. Journal of Neuroscience 2004; 24(-36-):7958-7963. >>> Brain-derived neurotrophic factor, recomb. human; CSF, artificial; CSF/CNS (hippocampus); Mice; 1007D; 2 weeks; Controls received mp w/ vehicle; replacement therapy (BDNF knockout mice); dose-response (fig. 7); pumps replaced at the end of the 1st week; no stress (see pg. 7960); brain tissue distribution; peptides; multiple pumps per animal (2); "all mice appeared well groomed and healthy and exhibited no abnormal behaviors in their home cage." (p. 7960).

P6867 Xu,B., Michalski,B., Racine,R.J., Fahnestock,M. The effects of brain-derived neurotrophic factor (BDNF) administration on kindling induction, Trk expression and seizure-related morphological changes. Neuroscience 2004; 126(-3-):521-531. >>> Brain-derived neurotrophic factor; cytochrome C; CSF/CNS (hippocampus); Rat; 2002; 14 days; Controls received mp w/ cytochrome C; comparison of bolus injections vs. mp; ALZET brain infusion kit used.

P6856 Frielingsdorf,H., Schwarz,K., Brundin,P., Mohapel,P. No evidence for new dopaminergic neurons in the adult mammalian substantia nigra. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-27-):10177-10182. >>> Uridine, bromodeoxy-; brain-derived neurotrophic factor; CSF/CNS; Rat; mice; 2002; 2004; 10,21 days; Brain tissue distribution.

P6806 Haninec,P., Dubovy,P., Samal,F., Houstava,L., Stejskal,L. Reinnervation of the rat musculocutaneous nerve stump after its direct reconnection with the C5 spinal cord segment by the nerve graft following avulsion of the ventral spinal roots: a comparison of intrathecal administration of brain-derived neurotrophic factor and Cerebrolysin. Experimental Brain Research 2004; 159(-4-):425-432. >>> Brain-derived neurotrophic factor; cerebrolysin; PBS; CSF/CNS (intrathecal); Rat; 2002; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; ALZET brain infusion kit 2 used; C5 ventral root avulsion; "indicating a superior availability and effect of neuron-promoting agents after their continuous intrathecal rather than single or intravenous administration even if their concentrations were lower." (p. 430).

P6770 Canals,J.M., Pineda,J.R., Torres-Peraza,J.F., Bosch,M., Martin-Ibanez,R., Munoz,M.T., Mengod,G., Ernfors,P., Alberch,J. Brain-derived neurotrophic factor regulates the onset and severity of motor dysfunction associated with enkephalinergic neuronal degeneration in Huntington's disease. Journal of Neuroscience 2004; 24(-35-):7727-7739. >>> Brain-derived neurotrophic factor; PBS; CSF/CNS (striatum); Mice (transgenic); 2001; 1 week; Controls received mp w/ vehicle; peptides; neurodegenerative (Huntington's disease).

P6652 Eisch,A.J., Bolanos,C.A., de Wit,J., Simonak,R.D., Pudiak,C.M., Barrot,M., Verhaagen,J., Nestler,E.J. Brain-derived neurotrophic factor in the ventral midbrain-nucleus accumbens pathway: A role in depression. Biological Psychiatry 2003; 54(-10-):994-1005. >>> Brain-derived neurotrophic factor, human recomb.; CSF/CNS (ventral tegmentum); Rat; 1007D; 1 week; Controls received mp w/ saline; no stress (see pg. 1001); post op. care (penicillin [IM], topical antibiotic); cannula placement and gross morphology verified at end of infusion period.

P6458 Olivius,P., Alexandrov,L., Miller,J.M., Ulfendahl,M., Bagger-Sjoebaeck,D., Kozlova,E.N. A model for implanting neuronal tissue into the cochlea. BRAIN RESEARCH PROTOCOLS 2004; 12(-3-):152-156. >>> Brain-derived neurotrophic factor; ciliary neurotrophic factor Ax1, human; Ear (scala tympani); Guinea-pig; 2002; 21-22 days; Pump replaced on days 11-12; post op. care (cyclosporine, doxycycline); tissue perfusion (scala tympani).

P6440 Matsunaga,W., Shirokawa,T., Isobe,K. BDNF is necessary for maintenance of noradrenergic innervations in the aged rat brain. NEUROBIOLOGY OF AGING 2004; 25(-3-):341-348. >>> Brain-derived neurotrophic factor; BDNF antibody-; immunoglobulin G; PBS; CSF/CNS (frontal cortex); Rat; 2002; 13-15 days; Controls received mp w/ vehicle; post op. care (antibiotic for 5 days after operation); cannula secured by dental cementl; BDNF dissolved in PBS (pH 7.4); brain tissue distribution was measured by biotin-labeled BDNF.

P6358 varez-Borda,B., Haripal,B., Nottebohm,F. Timing of brain-derived neurotrophic factor exposure affects life expectancy of new neurons. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-11-):3957-3961. >>> Brain-derived neurotrophic factor; CSF/CNS (high vocal center); Bird (canary); 1007D; 6 days; 23 g guide cannula used; pumps implanted IP with an external catheter; infusion schematic p. 3958; external pump application.

P6347 Kim,D.H., Jahng,T.A. Continuous brain-derived neurotrophic factor (BDNF) infusion after methylprednisolone treatment in severe spinal cord injury. Journal of Korean Medical Science 2004; 19(-1-):113-122. >>> Brain-derived neurotrophic factor; CSF, artificial; CSF/CNS (intrathecal); Rat; 2004; 6 weeks; ALZET brain infusion kit; spinal cord injury; behavorial study.

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2. Ciliary Neurotrophic Factor

R0239 Richardson,R.T., Noushi,F., O'Leary,S. Inner ear therapy for neural preservation. AUDIOLOGY AND NEURO-OTOLOGY 2006; 11(-6-):343-356. >>> Nerve growth factor; NT-3; adenovirus; brain-derived neurotrophic factor; perilymph, artificial; glial-derived neurotrophic factor; ciliary neurotrophic factor; fibroblast growth factor, acidic; fibroblast growth factor-1; fibroblast growth factor-2; fibroblast growth factor, basic; Ear (cochlea); ear (scala tympani); Guinea-pig; 1,2,4,8 weeks; 15-60, 11-12, 26 days; Comparison of polymers, hydrogels, gene therapy, cell-based therapy, and injections vs. mp; long-term study; pumps replaced; no stress (see pg. 350); half-life (p. 344), short in blood; gene therapy; peptides; animal info (deafened); Table 2; "The mini-osmotic pump device is ideally suited to studying the effects of neurotrophic factors in the cochlea experimentally." (p. 350).

P7953 Hagg,T., Varon,S. Ciliary Neurotrophic Factor Prevents Degeneration of Adult Rat Substantia Nigra Dopaminergic Neurons in vivo. Proceedings of the National Academy of Sciences 1993; 90(-13-):6315-6319. >>> Ciliary neurotrophic factor, recomb. human; CSF, artificial; albumin, rat serum; CSF/CNS (substantia nigra); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides; neurodegenerative (Parkinson's disease); animal info (female, Sprague-Dawley, 200 grams); lynch coil filled with vehicle or agent, mp filled with vehicle; mp partly coated with paraffin.

P7860 Pun,S., Santos,A.F., Saxena,S., Xu,L., Caroni,P. Selective vulnerability and pruning of phasic motoneuron axons in motoneuron disease alleviated by CNTF. Nature Neuroscience 2006; 9(-3-):408-419. >>> Ciliary neurotrophic factor, recomb. rat; glial-derived neurotrophic factor, recomb. human; PBS; BSA; IM (triceps surae); Mice (transgenic); 1002; ; Controls received mp w/ vehicle; functionality of mp verified by residual volume; peptides; neurodegenerative (motoneuron disease); animal info (Tg G93A SoD1, G85R S0D1).

P7558 Kelleher,M.O., Myles,L.M., Al-Abri,R.K., Glasby,M.A. The use of ciliary neurotrophic factor to promote recovery after peripheral nerve injury by delivering it at the site of the cell body. Acta Neurochirurgica 2006; 148(-1-):55-61. >>> Ciliary neurotrophic factor; CSF/CNS (intrathecal, subarachnoid space); Sheep; 2ML4; 28 days; Controls received mp w/ physiological saline; no stress (see pg. 57); half-life (pg. 59) 3 minutes in serum; peptides; post op. care (cefuroxime); median nerve transected and repaired; "The adverse cytokine-like side-effects associated with systemic administration of CNTF, namely cough, asthenia, nausea, anorexia and weight loss, aphthous stomatitis and fever were not observed in any of our animals". (pg. 57).

P7339 Yokota,H., Yoshikawa,M., Hirabayashi,H., Nakase,H., Uranishi,R., Nishimura,F., Sugie,Y., Ishizaka,S., Sakaki,T. Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha;) following experimental intracerebral hemorrhage in rats. Neuroscience Letters 2005; 377(-3-):170-175. >>> Ciliary neurotrophic factor; PBS; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; good methods p. 171; ALZET brain infusion kit.

P6761 McCallister,W.V., McCallister,E.L., Dubois,B., Trumble,T.E. Regeneration along intact nerves using nerve growth factor and ciliary neurotrophic factor. Journal of Reconstructive Microsurgery 2004; 20(-6-):473-481. >>> Nerve growth factor; ciliary neurotrophic factor; CSF/CNS (peroneal nerve); Rat; 2ML4; 28 days; Controls received mp w/ isotonic saline; peptides; mp primed 24 hours in 37 degrees Celsius saline.

P5990 Ramirez,B.U., Retamal,L., Vergara,C. Ciliary Neurotrophic Factor (CNTF) affects the excitable and contractile properties of innervated skeletal muscles. Biological Research 2003; 36(-3-4-):303-312. >>> Ciliary Neurotrophic Factor; Forskolin; PBS; BSA; SC; Rat; 2002; 14 days; Controls received mp w/ saline; control group pumps were implanted and contained saline; experimental groups had either CNTF or forskolin.

P5983 Zou,J., Bretlau,P., Pyykkoe,I., Toppila,E., Olovius,N.P., Stephanson,N., Beck,O., Miller,J.M. Comparison of the protective efficacy of Neurotrophins and antioxidants for vibration-induced trauma. ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES 2003; 65(-3-):155-161. >>> Brain-derived neurotrophic factor; ciliary neurotrophic factor; Ear (scala tympani); Guinea-pig; 2002; 14 days; tissue perfusion (cochlea); comparison of RWM injections vs. mp; peptides.

P5009 Shinohara,T., Bredberg,G., Ulfendahl,M., Pyykko,I., Olivius,N.P, Kaksonen,R., Lindstrom,B., Altschuler,R., Miller,J.M. Neurotrophic factor intervention restores auditory function in deafened animals. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2002; 99(-3-):1657-1660. >>> Brain-derived neurotrophic factor; Ciliary neurotrophic factor; Neomycin;; Perilymph, artificial; Ear (scala tympani); guinea-pig; 2002; 26 days; controls received mp w/ vehicle; tissue perfusion (cochlea, scala tympani); pumps replaced at day 15; peptides; catheter filled with perilymph and 10% neomycin; pump filled with vehicle or neurotrophic factor solution; 48-hr infusion of neomycin to cause deafness followed by 12 or 26 day infusion of neurotrophic factor pump reservoir..

P4362 Lee,T.T., Green,B.A., Dietrich,W.D., Yezierski,R.P. Neuroprotective effects of basic fibroblast growth factor following spinal cord contusion injury in the rat. Journal of Neurotrauma 1999; 16(-5-):347-356. >>> Interleukin-1; Interleukin-4; Interleukin-6; Fibroblast growth factor, basic; Nerve growth factor; Ciliary neurotrophic factor;; Water; PBS; BSA; CHAPS;; CSF/CNS (spinal cord, intrathecal);; rat;; 2001;; 7 days;; controls received mp with vehicle; tissue perfusion (injury site); good methods; peptides; 1:10 dilutional effect of CSF confirmed by dye study; PE10/50 tubing used with pump and 30 gauge needle to deliver drugs through small dural plial opening;.

P4338 Berhow,M.T., Hiroi,N., Kobierski,L.A., Hyman,S.E., Nestler,E.J. Influence of cocaine on the JAK-STAT pathway in the mesolimbic dopamine system. Journal of Neuroscience 1996/12/15; 16(-24-):8019-8026. >>> Ciliary neurotrophic factor; Brain-derived neurotrophic factor;; Sodium phosphate; Saline; BSA;; CSF/CNS (ventral tegmental area);; rat;; 2002;; ; controls received mp with vehicle; functionality of CNTF delivery via mp verified by weight loss and increased TH levels in VTA; peptides; recomb. human growth factor used;.

P4330 Berhow,M.T., Hiroi,N., Nestler,E.J. Regulation of ERK (extracellular signal regulated kinase), part of the neurotrophin signal transduction cascade, in the rat mesolimbic dopamine system by chronic exposure to morphine or cocaine;. Journal of Neuroscience 1996; 16(-15-):4707-4715. >>> Brain-derived neurotrophic factor; Ciliary neurotrophic factor; Oligonucleotide, phosphorothioate antisense; AP-5, D-; AP-5, L-;; Sodium phosphate; Saline; BSA;; CSF/CNS (ventral tegmental area);; rat;; 2002;; ; controls received mp with vehicle or sense or scrambled oligo; comparison of acute injections vs. mp; peptides; antisense; human recomb. growth factors used;.

P3906 Lewin,S.L., Utley,D.S., Cheng,E.T., Verity,A.N., Terris,D.J. Simultaneous treatment with BDNF and CNTF after peripheral nerve transection and repair enhances rate of functional recovery compared with BDNF treatment alone. Laryngoscope 1997; 107(--):992-999. >>> Brain-derived neurotrophic factor; Ciliary neurotrophic factor; Ringer's solution, lactated; CSF/CNS (sciatic nerve); rat; 2ML4; 30 days; tissue perfusion (sciatic nerve): functionality of mp verified by residual volume; stress/adverse reaction: cannula extrusion, problems in denervated hindpaws; peptides; agents given singly and concomitantly in same pump.

P3833 Marques,M.J., Neto,H.S. Ciliary neurotrophic factor stimulates in vivo myotube formation in mice. Neurosci.Lett. 1997; 234(--):43-46. >>> Ciliary neurotrophic factor; PBS; IM; mice; 2001; 8 days; controls received mp w/mouse serum and PBS; tissue perfusion (tibialis anterioris muscle); peptides; recomb. rat CNTF used.

P3732 Miller,J.M., Chi,D.H., O'Keeffe,L.J., Kruszka,P., Raphael,Y., Altschuler,R.A. Neurotrophins can enhance spiral ganglion cell survival after inner hair cell loss. Int.J.Devl.Neuroscience 1997; 15(-4/5-):631-643. >>> Brain-derived neurotrophic factor; NT-3; Glial-derived neurotrophic factor; Ciliary neurotrophic factor; Perilymph, artificial;; Ear (cochlea);; guinea-pig;; 2 weeks; 28 days;; controls received mp with AP; pumps replaced after 7 days; tissue perfusion (scala tympani); peptides;.

P3425 Newman,J.P., Verity,A.N., Hawatmeh,S., Fee Jr,W.E., Terris,D.J. Ciliary neurotrophic factor enhances peripheral nerve regeneration. Arch Otolaryngol.Head Neck Surg. 1996; 122(--):399-403. >>> Ciliary neurotrophic factor; Nerve growth factor; Water, sterile; CSF/CNS (sciatic nerve); rat; 2ML4; 4 weeks; controls received Ringer's solution, lactated; tissue perfusion (nerve); peptides.

P2650 Zhang,F., Richardson,P.M., Holland,D.P., Guo,Q., Tatton,W.G. CNTF or (-)-deprenyl in immature rats: survival of axotomized facial motoneurons and weight loss. J.Neurosci.Res. 1995; 40(--):564-570. >>> Ciliary neurotrophic factor; Saline; Albumin, bovine serum; CSF/CNS (spinal cord, intrathecal); CSF/CNS (extrathecal-by cranio-cervical junction); rat; 1007D; 7 days; controls received mp w/ vehicles; dose-response; stress/adverse reaction: weight loss; peptides; CNTF given intrathecally and extrathecally; recomb. rat CNTF used.

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3. Glial-derived Neurotrophic Factor

Q0778 Voutilainen,M.H., Baeck,S., Peraenen,J., Lindholm,P., Raasmaja,A., Maennistoe,P.T., Saarma,M., Tuominen,R.K. Chronic infusion of CDNF prevents 6-OHDA-induced deficits in a rat model of Parkinson's disease. Experimental Neurology 2011; 228(-1-):99-108. >>> Central dopmaine neurotrophic factor; mesencephalic astrocyte-derived neurotrophic factor; glial-derived neurotrophic factor; PBS; CSF/CNS (striatum); Rat; 2002; 3, 14 days; Controls received mp w/ vehicle; animal info (male, Wistar, 250-280 g); neuroprotection.

Q0275 Li,J.H., Chian,R.J., Ay,I., Kashi,B.B., Celia,S.A., Tamrazian,E., Pepinsky,R.B., Fishman,P.S., Brown,RH Jr, Francis,J.W. Insect GDNF:TTC fusion protein improves delivery of GDNF to mouse CNS. Biochemical and Biophysical Research Communications 2009; 390(-3-):947-951. >>> Glial-derived neurotrophic factor, recombinant, rat; glial-derived neurotrophic factor, TTC; HEPES; NaCl; BSA; CSF/CNS; Mice; 1 week; Controls received mp w/ vehicle; animal info (normal, male, C57BL6, 8-10-wks-old, 25-30 g); GDNF:TTC also known as glial-derivied neurotrophic factor: tetanus toxin C-fragment fusion protein.

Q0059 Ubhi,K., Rockenstein,E., Mante,M., Inglis,C., Adame,A., Patrick,C., Whitney,K., Masliah,E. Neurodegeneration in a Transgenic Mouse Model of Multiple System Atrophy Is Associated with Altered Expression of Oligodendroglial-Derived Neurotrophic Factors. Journal of Neuroscience 2010; 30(-18-):6236-6246. >>> Glial-derived neurotrophic factor; CSF/CNS; Mice; 1002; 14 days; Animal info (MBP-h alpha syn Tg, 8 months old).

P9872 Unezaki,S., Yoshii,S., Mabuchi,T., Saito,A., Ito,S. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. Journal of Neuroscience Methods 2009; 178(-2-):308-315. >>> Nerve growth factor; glial-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Mice (transgenic); 1004; 4 weeks; Controls received mp w/ vehicle; half-life (p. 308) "short"; animal info (10 wks old, 20 g., Thy1-YEP); image of pump pg. 309; schematic of drug delivery system, fig. 1); "Because of the short biological half-life of neurotrophic factors, a delivery system that protects the protein and slowly releases it locally over a prolonged period of time is required." pg. 308; tissue perfusion (sciatic nerve).

P9515 Scheper,V., Paasche,G., Miller,J.M., Warnecke,A., Berkingali,N., Lenarz,T., Stoever,T. Effects of Delayed Treatment With Combined GDNF and Continuous Electrical Stimulation on Spiral Ganglion Cell Survival in Deafened Guinea Pigs. Journal of Neuroscience Research 2009; 87(-6-):1389-1399. >>> Glial-derived neurotrophic factor; Ear (cochlea); Guinea-pig; 2002; 48 days; Controls received mp w/artificial perilymph; tissue perfusion (cochlea); long-term study; pumps replaced after 13 days; good methods (pg. 1391); animal info (male, pigmented, 250-450 g.); pumps primed; image of pump and electrode cannula device used on fig. 1.

P9441 Sakai,A., Asada,M., Seno,N., Suzuki,H. Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain. Pain 2008; 137(-2-):378-388. >>> Oligodeoxynucleotide, phosphorothioate antisense; glial-derived neurotrophic factor, recomb. human; neural cell adhesion molecule, mimetic peptide C3d; oligodeoxynucleotide, phosphorothioate missense; CSF/CNS (subarachnoid space); Rat; 4, 6, 7, 9 days; Controls received mp w/ saline; antisense (NCAM); peptides; multiple pumps per animal (2); animal info (male, Sprague Dawley, 5 wks old, 140-160 g., sciatic nerve injury); behavioral testing (mechanical allodynia, thermal hypersensitivity, paw withdrawal, plantar test).

P9324 Averill,S., Inglis,J.J., King,V.R., Thompson,S.W.N., Cafferty,W.B.J., Shortland,P.J., Hunt,S.P., Kidd,B.L., Priestley,J.V. Reg-2 expression in dorsal root ganglion neurons after adjuvant-induced monoarthritis. Neuroscience 2008; 155(-4-):1227-1236. >>> Nerve growth factor, recomb. human; glial-derived neurotrophic factor, recomb. human; leukemia inhibitory factor, recomb. human; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides, animal info (male, Wistar, 220-400 g.).

P9156 Du,Y.L., Li,X.P., Yang,D.H., Zhang,X.J., Chen,S., Huang,K.X., Le,W.D. Multiple molecular pathways are involved in the neuroprotection of GDNF against proteasome inhibitor induced dopamine neuron degeneration in vivo. EXPERIMENTAL BIOLOGY AND MEDICINE 2008; 233(-7-):881-890. >>> Glial-derived neurotrophic factor, recomb.; CSF, artificial; CSF/CNS (striatum); Mice; 1002; 2 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit used; animal info (male, C57BL/6, 8 wks old); neurodegenerative (Parkinson's disease).

P7483 Chen,Y., Ai,Y., Slevin,J.R., Maley,B.E., Gash,D.M. Progenitor proliferation in the adult hippocampus and substantia nigra induced by glial cell line-derived neurotrophic factor. Experimental Neurology 2005; 196(-1-):87-95. >>> Glial-derived neurotrophic factor, recomb. human methionyl; CSF, artificial; CSF/CNS (striatum); Rat; 28 days; Controls received mp w/ vehicle; brain tissue distribution; animal info (male, Sprague-Dawley, 2 month old).

P6801 Iannotti,C., Zhang,Y.P., Shields,C.B., Han,Y.C., Burke,D.A., Xu,X.M. A neuroprotective role of glial cell line-derived neurotrophic factor following moderate spinal cord contusion injury. Experimental Neurology 2004; 189(-2-):317-332. >>> Glial-derived neurotrophic factor, recomb. human; glial-derived neurotrophic factor, alexa S94 dye-conjugated; Saline; CSF/CNS (intrathecal); Rat; 2004; 3 weeks; 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume and cannula patency; good methods p. 318; brain tissue distribution; spinal cord injury.

P6644 Bergerot,A., Shortland,P.J., Anand,P., Hunt,S.P., Carlstedt,T. Co-treatment with riluzole and GDNF is necessary for functional recovery after ventral root avulsion injury. Experimental Neurology 2004; 187(-2-):359-366. >>> Glial-derived neurotrophic factor, isr-met human; CSF/CNS (intrathecal); Rat; 2002; 2 weeks; Controls received no treatment to contralateral side.

P6602 Tolbert,D.L., Clark,B.R. GDNF and IGF-I trophic factors delay hereditary Purkinje cell degeneration and the progression of gait ataxia. Experimental Neurology 2003; 183(-1-):205-219. >>> Glial-derived neurotrophic factor; insulin-like growth factor I; SC; Rat; 2004; 4,8 weeks; Controls received mp w/ saline; long-term study; pumps replaced at 4 weeks; peptides.

P6592 Dempsey,R.J., Sailor,K.A., Bowen,K.K., Tuereyen,K., Vemuganti,R. Stroke-induced progenitor cell proliferation in adult spontaneously hypertensive rat brain: effect of exogenous IGF-1 and GDNF. Journal of Neurochemistry 2003; 87(-3-):586-597. >>> Insulin-like growth factor I; glial-derived neurotrophic factor; CSF, artificial; CSF/CNS; Rat; 1 week; Controls received mp w/ vehicle; ALZET brain infusion kit used; peptides; ischemia (cerebral); MCAO.

P6506 Nagano,M., Sakai,A., Takahashi,N., Umino,M., Yoshioka,K., Suzuki,H. Decreased expression of glial cell line-derived neurotrophic factor signaling in rat models of neuropathic pain. British Journal of Pharmacology 2003; 140(-7-):1252-1260. >>> Glial-derived neurotrophic factor, recomb. human; Saline; CSF/CNS (intrathecal); Rat; 9 days; Controls received mp w/ vehicle; replacement therapy (spinal nerve ligation axotomy); post op. care (IP kanamycin sulfate).

P6343 Wang,R., Guo,W., Ossipov,M.H., Vanderah,T.W., Porreca,F., Lai,J. Glial cell line-derived neurotrophic factor normalized neurochemical chances in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain. Neuroscience 2003; 121(-3-):815-824. >>> Glial-derived neurotrophic factor, recomb. human; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2002; 10 days; Controls received mp w/ vehicle; spinal nerve ligation injury.

P6221 Hess,B.H., Krewet,J.A., Tolbert,D.L. Olivocerebellar projections are necessary for exogenous trophic factors to delay heredo-Purkinje cell degeneration. Brain Research 2003; 986(-1-2-):54-62. >>> Glial-derived neurotrophic factor; insulin-like growth factor I; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ saline or no treatment; ALZET brain infusion kit used.

P5967 Boyd,J.G., Gordon,T. Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneurons in vivo. Experimental Neurology 2003; 183(-2-):610-619. >>> Glial-derived neurotrophic factor; brain-derived neurotrophic factor; Saline; CSF/CNS (tibial nerve); Rat; 2001; 2ML4; 7,28 days; Controls received mp w/ vehicle; tissue perfusion (nerve repair site); good methods (p. 611,612); silastic cuff used; pumps used for a 1 week and 4 week study.

P5858 Ramer,M.S., Bradbury,E.J., McMahon,S.B. Nerve growth factor induces P2X(3) expression in sensory neurons. J Neurochem 2001; 77(-3-):864-875. >>> Nerve growth factor; Glial-derived neurotrophic factor; Saline; rat serum; CSF/CNS (intrathecal); Rat; 2001; 1 week; Peptides.

P5845 Akkina,S.K., Patterson,C.L., Wright,D.E. GDNF rescues nonpeptidergic unmyelinated primary afferents in streptozotocin-treated diabetic mice. Exp Neurol 2001; 167(-1-):173-182. >>> Nerve growth factor; Glial-derived neurotrophic factor; CSF, artificial; CSF/CNS (intrathecal); Mice; 1002; 14 days; Controls received mp w/ vehicle; comparison of intrathecal injections vs. mp; stress/adverse reaction (see pg. 178, surgical complications); peptides; PE-10 attached to PE-60 tubing; catheter placement verified at sacrifice.

P5837 Li,S.L., Xu,B., Martin,D., Racine,R.J., Fahnestock,M. Glial cell line-derived neurotrophic factor modulates kindling and activation-induced sprouting in hippocampus of adult rats. Experimental Neurology 2002; 178(-1-):49-58. >>> Glial-derived neurotrophic factor; Cytochrome C; Citrate butter; CSF/CNS; Rat; 2002; 11 days; Peptides; 7 day recovery from surgery.

P5588 Leffler,A., Cummins,T.R., Dib-Hajj,S.D., Hormuzdiar,W.N., Black,J.A., Waxman,S.G. GDNF and NGF reverse changes in repriming of TTX-sensitive Na+ currents following axotomy of dorsal root ganglion neurons. Journal of Neurophysiology 2002; 88(-2-):650-658. >>> Glial-derived neurotrophic factor; Nerve growth factor; Saline; CSF/CNS (intrathecal, lumbar); rat; 7 days; controls received mp with vehicle; peptides; some animals received both agents infused simultaneously by the same pump; delivery was to the lumbar enlargement.

P4887 Issa,P.C., Lever,I.J., Michael,G.J., Bradbury,E.J., Malcangio,M. Intrathecally delivered glial cell line-derived neurotrophic factor produces electrically evoked release of somatostatin in the dorsal horn of the spinal cord. Journal of Neurochemistry 2001; 78(--):221-229. >>> Glial-derived neurotrophic factor, recombinant human; Saline; Albumin, rat serum; CSF/CNS (intrathecal); rat; 2001; 11-13 days; controls received mp w/ vehicle; peptides; vehicle was saline w/ 0.1% rat serum albumin; neuroprotection; no mention of pump replacement for 11-13 day delivery..

P4850 Tolbert,D.L., Bradley,M.W., Tolod,E.G., Torres-Aleman,I., Clark,B.R. Chronic intraventricular infusion of glial cell line-derived neurotrophic factor (GDNF) rescues some cerebellar Purkinje cells from heredodegeneration. Experimental Neurology 2000; 170(--):375-379. >>> Glial-derived neurotrophic factor; Saline; CSF/CNS; rat; 2004; 4 weeks; controls received mp w/ vehicle; peptides; brain distribution; aging; neuroprotection..

P4798 Kirik,D., Georgievska,B., Rosenblad,C., Bjorklund,A. Delayed infusion of GDNF promotes recovery of motor function in the partial lesion model of Parkinson's disease.. European Journal of Neuroscience 2001; 13(--):1589-1599. >>> Glial-derived neurotrophic factor;; Saline; Serum, rat; Gentamycin;; CSF/CNS (striatum); CSF/CNS;; rat;; 2002;; 4 weeks;; Controls received mp w/ vehicle; pumps replaced after 2 weeks; ALZET brain infusion kit used; peptides; neurodegenerative (Parkinson's disease); neuroprotection;.

P4769 Ramer,M.S, Priestley,J.V., McMahon,S.B. Functional regeneration of sensory axons into the adult spinal cord.. Nature 2000; 403(--):312-316. >>> Nerve growth factor; NT-3; Brain- derived neurotrophic factor; Glial-derived neurotrophic factor;; Albumin, rat serum; Saline, sterile;; CSF/CNS (intrathecal);; rat;; 7 days;; Controls received mp w/ vehicle; peptides; rats had received complete crush injuries of 2-3 cervical dorsal roots (rhizotomized);.

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4. NT-3

P9183 Wilson-Gerwing,T.D., Stucky,C.L., McComb,G.W., Verge,V.M.K. Neurotrophin-3 significantly reduces sodium channel expression linked to neuropathic pain states. Experimental Neurology 2008; 213(-2-):303-314. >>> NT-3; PBS; albumin, serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Post op. care (buprenorphine); animal info (male, Wistar, young adult, 250-300 g.).

P8038 Wilson-Gerwing,T.D., Verge,V.M.K. Neurotrophin-3 attenuates galanin expression in the chronic constriction injury model of neuropathic pain. Neuroscience 2006; 141(-4-):2075-2085. >>> NT-3; PBS; albumin, rat serum; streptomycin; penicillin; CSF/CNS (intrathecal, lumbar); Rat; 2001; 7 days; Post op. care (buprenorphine); animal info (adult, male, wistar, 250-300 grams).

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5. NT-4-5

P8676 Royo,N.C., LeBold,D., Magge,S.N., Chen,I., Hauspurg,A., Cohen,A.S., Watson,D.J. Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function. Neuroscience 2007; 148(-2-):359-370. >>> NT-4/5, recomb. human; PBS; BSA; CSF/CNS (somatosensory cortex); 1007D; ; Controls received mp w/ vehicle; tissue perfusion (cortex); good methods (p. 360); ALZET brain infusion kit 2 used; brain tissue distribution; post op. care (recovery on heating pad); animal info (male, Sprague-Dawley, 350-400g); 0.1% BSA; traumatic brain injury.

P8389 Jabbar,S., Harada,F., Aita,M., Ohishi,M., Saito,I., Kawano,Y., Suzuki,A., Nozawa-Inoue,K., Maeda,T. Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage. Journal of Comparative Neurology 2007; 501(-3-):400-412. >>> NT-4/5, recomb.; Saline, physiological; SC; Mice; 1002; 7, 14 days; Controls received mp w/ vehicle, or no treatment; replacement therapy (nt-4-5 knock out); peptides; animal info (12 wks old, 20-28g.); inferior alveolar nerve transection.

P6328 Cabelli,R.J., Shelton,D.L., Segal,R.A., Shatz,C.J. Blockade of Endogenous Ligands of TrkB Inhibits Formation of Ocular Dominance Columns. Neuron 1997; 19(--):63-76. >>> Immunoglobulin G, TrkA; immunoglobulin, TrkB; immunoglobulin G, TrkC; NT-4/5; NT-3; BSA; PBS, sterile; CSF/CNS (visual cortex); Cat; ferret; 2002; 2,3 weeks; 2 days; Controls received mp w/ vehicle, or IgG protein fusions unrelated to Trk; comparison of CSF/CNS injections vs. mp; stability verified by Ab immunostaining of brain sections; peptides; post op. care (7 days oral amoxicillin); 3 week infusions had a flow rate of approx. 0.33 ul/hr, "the reduced flow rate (~0.33 ul/hr) was achieved by coating ~1/3 of the surface area of the minipump with hot paraffin prior to filling") p. 74).

P6243 Viegi,A., Cotrufo,T., Berardi,N., Mascia,L., Maffei,L. Effects of dark rearing on phosphorylation of neurotrophin Trk receptors. European Journal of Neuroscience 2002; 16(-10-):1925-1930. >>> Nerve growth factor; brain-derived neurotrophic factor, recomb. human; NT-4; CSF/CNS (visual cortex); Rat; 8 days; Controls received mp w/ cytochrome c; peptides.

P5632 Wahle,P., Di Cristo,G., Schwerdtfeger,G., Engelhardt,M., Berardi,N., Maffei,L. Differential effects of cortical neurotrophic factors on development of lateral geniculate nucleus and superior colliculus neurons: anterograde and retrograde actions. Development 2003; 130(-3-):611-622. >>> Nerve growth factor; NT-4/5; brainderived neurotrophic factor; leukemia inhibitory factor; CSF/CNS (visual cortex); Rat; 1007D; 7 days; Controls received mp w/ cytochrome C; peptides..

P5537 Lodovichi,C., Berardi,N., Pizzorusso,T., Maffei,L. Effects of neurotrophins on cortical plasticity: same or different?. J.Neurosci. 2000/3/15; 20(-6-):2155-2165. >>> Nerve growth factor; NT-3; NT-4; brain-derived neurotrophic factor; cytochrome C; CSF/CNS (visual cortex); Rat; 1007D; 1 week; Functionality of mp verified by detection of neurotrophins by immunohistochemistry; peptides; brain tissue distribution; mouse NGF, human recomb. NT-3 AND NT-4, and human recomb. BDNF used.

P5232 Hagg,T. Neurotrophins prevent death and differentially affect tyrosine hydroxylase of adult rat nigrostriatal neurons in vivo. Exp.Neurol. 1998/1; 149(-1-):183-192. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-3; NT-4; PBS; Albumin, rat serum; Buffer, sodium acetate; CSF/CNS (nigrostriatal complex); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides: stability (p.184); "at 1 mg/ml these factors retain more than 70% of their activity after 14 days at 37 C in ALZET pumps"; pumps partially coated with paraffin to reduce flow rate to 0.25 ul/hr; lynch coil used; neuronal survival; neurodegenerative (Parkinson's disease).

P4747 Kernie,S.G, Liebl,D.J, Parada,L.F. BDNF regulates eating behavior and locomotor activity in mice.. The European Molecular Biology Organization Journal 2000; 19(-6-):1290-1300. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-4/5; PBS; CSF/CNS (third ventricle); Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume after sacrifice; peptides; ALZET brain infusion kit used; brain cannula secured to skull by cyanoacrylate adhesive (Loctite 454); cannula patency verified by methlene blue injection after sacrifice; recomb. human BDNF used; all neurotrophins infused via same pump;.

P4714 Gillespie,D.C, Crair,M.C, Stryker,M.P. Neurotrophin-4/5 alters responses and blocks the effect of monocular deprivation in cat visual cortex during the critical period.. The Journal of Neuroscience 2000; 20(-24-):9174-9186. >>> NT-4/5; NT-3; Nerve growth factor;; PBS; BSA; Sodium acetate;; CSF/CNS (visual cortex);; cat; cat (kitten);; 1007D; 2001;; 7 days;; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 9175) some cannulae were found to be obstructed at the end of experiment; peptides; cannulae patency verified at the end of experiment;.

P4391 Kobayashi,N.R., Fan,D.-P., Giehl,K.M., Bedard,A.M., Wiegand,S.J., Tetzlaff,W. BDNF and NT-4/5 prevent atrophy of rat rubrospinal neurons after cervical axotomy, stimulate GAP-43 and Ta1-tubulin mRNA expression, and promote axonal regeneration. Journal of Neuroscience 1997/12/15; 17(-24-):9583-9595. >>> Nerve growth factor; Brain-derived neurotrophic factor; NT-3; NT-4/5;; PBS, sterile; Albumin, rat serum; Penicillin; Streptomycin;; CSF/CNS (red nucleus);; rat;; 2001;; 7 days;; controls received mp with vehicle or no pump; tissue perfusion (rubrospinal neurons); peptides; brain tissue distribution of neurotrophins assessed by immunohistochemistry (p. 9587); schematic of model, p. 9587; recomb. human NGF, BDNF, NT-4/5, and NT-3 used;.

P4385 Fernandes,K.J.L., Kobayashi,N.R., Jasmin,B.J., Tetzlaff,W. Acetylcholinesterase gene expression in axotomized rat facial motoneurons is differentially regulated by neurotrophins: Correlation with trkB and trkC mRNA levels and isoforms. Journal of Neuroscience 1998/12/1; 18(-23-):9936-9947. >>> Nerve growth factor; NT-3; NT-4/5; Brain-derived neurotrophic factor;; Saline; Buffer, sodium phosphate; Albumin, rat serum; Penicillin; Streptomycin;; CSF/CNS (facial nerve);; rat;; 2001;; 7 days;; controls received mp with vehicle; tissue perfusion (nerve stump); dose-response for BDNF (p. 9939); peptides; some animals underwent bilateral axotomy, with one pump infusing each side; silastic tubing was sutured in place over nerve stump, and adhesive prevented leakage from tubing;.

P4264 Mendell,L.M., Johnson,R.D., Munson,J.B. Neurotrophin modulation of the monosynaptic reflex after peripheral nerve transection. Journal of Neuroscience 1999/4/15; 19(-8-):3162-3170. >>> NT-3; NT-4/5; Mannitol; Sucrose; Histidine;; CSF/CNS (gastocnemius nerve); cat; 2ML2; 2ML4;; 8-35 days; axotomized nerve controls received mp w/ vehicle or SC NT-3; tissue perfusion (medial gastrocnemius nerve); good methods (p. 3163); peptides; schematic of delivery model (p. 3163); 10 mm Gore-tex sleeve on nerve was connected via silastic tubing to pump.

P4259 Clarke,D.B., Bray,G.M., Aguayo,A.J. Prolonged administration of NT-4/5 fails to rescue most axotomized retinal ganglion cells in adult rats. Vision Research 1998; 38(--):1517-1524. >>> NT-4/5; PBS; Eye (vitreous chamber); rat; 2002; 14,28 days; controls received mp w/vehicle; comparison of injections vs. mp; peptides.

P3667 Munson,J.B., Shelton,D.L., McMahon,S.B. Adult mammalian sensory and motor neurons: roles of endogenous neurotrophins and rescue by exogenous neurotrophins after axotomy. Journal of Neuroscience 1997/1/1; 17(-1-):470-476. >>> Fusion protein; NT-3; NT-4/5;; Acetate; Saline;; CSF/CNS (gastrocnemius nerve); CSF/CNS (fibial nerve);; rat;; 2002;; 2 weeks;; controls received mp with vehicle; tissue perfusion (nerve stump;) tissue perfusion (intact nerve); peptides; schematic of delivery model (p. 471);.

P3483 Sawai,H., Clarke,D.B., Kittlerova,P., Bray,G.M., Aguayo,A.J. Brain-derived neurotrophic factor and neurotrophin-4/5 stimulate growth of axonal branches from regenerating retinal ganglion cells. J.Neurosci. 1996; 16(-12-):3887-3894. >>> NT-4/5; PBS; Eye (vitreous chamber); rat; 2002; 14 days; comparison of intraocular injections vs. mp.

P3413 Cabelli,R.J., Hohn,A., Shatz,C.J. Inhibition of ocular dominance column formation by infusion of NT-4/5 or BDNF. Science 1995; 267(--):1662-1666. >>> Brain-derived neurotrophic factor; Nerve growth factor; NT-4/5; NT-3; CSF/CNS (primary visual cortex); cat; 8,14,15,16 days; peptides.

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6. Nerve Growth Factor

Q0668 Beguin,P.C., El-Helou,V., Gillis,M.A., Duquette,N., Gosselin,H., Brugada,R., Villeneuve,L., Lauzier,D., Tanguay,J.F., Ribuot,C., Calderone,A. Nestin((+)) Stem Cells Independently Contribute to Neural Remodelling of the Ischemic Heart. Journal of Cellular Physiology 2011; 226(-5-):1157-1165. >>> Nerve growth factor, 2.5S; SC; Rat; 2ML1; 1 week; Controls received mp w/ saline; animal info (adult, male, Sprague-Dawley).

Q0682 Cirillo,G., Bianco,M.R., Colangelo,A.M., Cavaliere,C., Daniele,D., Zaccaro,L., Alberghina,L., Papa,M. Reactive astrocytosis-induced perturbation of synaptic homeostasis is restored by nerve growth factor. NEUROBIOLOGY OF DISEASE 2011; 41(-3-):630-639. >>> Nerve growth factor, beta, recomb.; GM6001; CSF, artificial; albumin, rat serum; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (Sprague-Dawley, male, 250-300 g); enzyme inhibitor (metalloproteinase); PE10 connected to PE60 tubing.

Q0685 Toda,S., Sakai,A., Ikeda,Y., Sakamoto,A., Suzuki,H. A local anesthetic, ropivacaine, suppresses activated microglia via a nerve growth factor-dependent mechanism and astrocytes via a nerve growth factor-independent mechanism in neuropathic pain. Molecular Pain 2011; 7(-;-):U1-U11. >>> Nerve growth factor, beta, recomb., rat; Saline; albumin, rat, serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 220-300 g); chronic constrictive injury (CCI).

Q0009 Cirillo,G., Cavaliere,C., Bianco,M.R., De Simone,A., Colangelo,A.M., Sellitti,S., Alberghina,L., Papa,M. Intrathecal NGF Administration Reduces Reactive Astrocytosis and Changes Neurotrophin Receptors Expression Pattern in a Rat Model of Neuropathic Pain. Cellular and Molecular Neurobiology 2010; 30(-1-):51-62. >>> Nerve growth factor, b-, recomb. rat; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (adult, male, Sprague-Dawley, 250-300 g); SCI; spinal cord injury; behavioral testing (thermal nociceptive testing, mechanical allodynia).

Q0129 Goda,M., Atagi,S., Amitani,K., Hobara,N., Kitamura,Y., Kawasaki,H. Nerve Growth Factor Suppresses Prostate Tumor Growth. JOURNAL OF PHARMACOLOGICAL SCIENCES 2010; 112(-4-):463-466. >>> Nerve growth factor; SC; Mice (nude); 1002; 2 weeks; Controls received mp w/ saline; cancer (prostate); animal info (BALB/c, Slc, 5 wks old, nu/nu).

Q0157 Lazo,O.M., Mauna,J.C., Pissani,C.A., Inestrosa,N.C., Bronfman,F.C. Axotomy-induced neurotrophic withdrawal causes the loss of phenotypic differentiation and downregulation of NGF signalling, but not death of septal cholinergic neurons. Molecular Neurodegeneration 2010; 5(-;-):U1-U12. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (Sprague Dawley, 280-300 g.); aCSF recipe; incorrectly stated 2002 pump.

Q0468 Conner,J.M., Franks,K.M., Titterness,A.K., Russell,K., Merrill,D.A., Christie,B.R., Sejnowski,T.J., Tuszynski,M.H. NGF Is Essential for Hippocampal Plasticity and Learning. Journal of Neuroscience 2009; 29(-35-):10883-10889. >>> Nerve growth factor, recomb. human; CSF, artificial; albumin, rat; CSF/CNS (septum); Rat; 2002; ; Controls received mp w/ vehicle; animal info (adult, female F344, 175-200 g, 3-4 months old); behavioral testing (Morris water maze test).

P9539 Xanthos,D.N., Kumar,N., Theodorsson,E., Coderre,T.J. The roles of nerve growth factor and cholecystokinin in the enhancement of morphine analgesia in a rodent model of central nervous system inflammation. Neuropharmacology 2009; 56(-3-):684-691. >>> Nerve growth factor, beta; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Long Evans, hooded).

P9466 Xing,J.H., Lu,J., Li,J.H. Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1380-H1387. >>> Nerve growth factor; SC; Rat; 1003D; 72 hours; Controls received mp w/ saline to contralateral leg; cardiovascular; peptides; multiple pumps per animal (2); ischemia (muscle); animal info (male, Sprague Dawley, 5-7 wks old); SC in the hindlimb.

Q0544 Hobara,N., Yoshida,N., Goda,M., Yokomizo,A., Kitamura,Y., Sendou,T., Kawasaki,H. Neurotrophic Effect of Hepatic Growth Factor (HGF) on Reinnervation of Perivascular Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves Following Phenol-Induced Nerve Injury in the Rat Mesenteric Artery. JOURNAL OF PHARMACOLOGICAL SCIENCES 2008; 108(-4-):495-504. >>> Hepatic growth factor, human; nerve growth factor; PBS; Triton-X; saline, sterile; IP; Rat; 2001; 7 days; Controls received mp w/ saline; animal info (8 wks old, Wistar).

P9004 Clark,R.S.B., Nathaniel,P.D., Zhang,X.P., Dixon,C.E., Alber,S.M., Watkins,S.C., Melick,J.A., Kochanek,P.M., Graham,S.H. boc-Aspartyl(OMe)-fluoromethylketone attenuates mitochondrial release of cytochrome c and delays brain tissue loss after traumatic brain injury in rats. Journal of Cerebral Blood Flow and Metabolism 2007; 27(-2-):316-326. >>> Nerve growth factor; CSF, artificial; albumin, mouse; CSF/CNS (parietal cortex); Rat; 2002; 14 days; Controls received mp w/ vehicle; no stress (see pg. 321); peptides; cardiovascular; animal info (male, Sprague Dawley, 280-400 g., CCI brain injury); behavioral testing (beam balance, beam walking, Morris water maze).

P8890 Hobara,N., Goda,M., Yoshida,N., Takatori,S., Kitamura,Y., Mio,M., Kawasaki,H. Angiotensin II type 2 receptors facilitate reinnervation of phenol-lesioned vascular calcitonin gene-related peptide-containing nerves in rat mesenteric arteries. Neuroscience 2007; 150(-3-):730-741. >>> Angiotensin II; nerve growth factor; PD123319; losartan; Saline; water; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; peptides; post op. care (penicillin); animal info (8 wks old, Wistar).

P8426 Hobara,N., Goda,M., Kitamura,Y., Sendou,T., Gomita,Y., Kawasaki,H. Adrenomedullin facilitates reinnervation of phenol-injured perivascular nerves in the rat mesenteric resistance artery. Neuroscience 2007; 144(-2-):721-730. >>> Adrenomedullin, recomb. human; nerve growth factor; Saline, sterile; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig. 2); peptides; animal info (Wistar, 8 wk old, perivascular denervation).

P8526 Randolph,C.L., Bierl,M.A., Isaacson,L.G. Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input. Brain Research 2007; 1144(--):59-69. >>> Nerve growth factor, mouse; Bisbenzimide; CSF/CNS; Rat; 2002; 2 weeks; Controls received no treatment; peptides; animal info (female, Sprague-Dawley, 3 months old); bisbenzimide (fluorescent marker) added to infusate to monitor cannula placement.

P7582 Cardoso,A., Paula-Barbosa,M.M., Lukoyanov,N.V. Reduced density of neuropeptide Y neurons in the somatosensory cortex of old male and female rats: Relation to cholinergic depletion and recovery after nerve growth factor treatment. Neuroscience 2006; 137(-3-):937-948. >>> Dye, methylene blue; nerve growth factor; CSF, artificial; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle or sham surgery; functionality of mp verified by pretesting; Alzet brain infusion kit used; post op. care (antiseptic); animal info (male, female, Wistar 6 month old, 24 month old); Lynch coil filled with NGF, air-oil spacer between mp and catheter, mp filled with dye.

P7728 Frielingsdorf,H., Thal,L.J., Pizzo,D.P. The septohippocampal cholinergic system and spatial working memory in the Morris water maze. Behavioural Brain Research 2006; 168(-1-):37-46. >>> Nerve growth factor, recomb. human; CSF, artificial; gentamicin; albumin, rat serum; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; cannula placement confirmed by cresyl violet staining; peptides; animal info (male, Fisher 344, 13-14 wk. old, medial septum lesion, 400g); mp primed 2 days in 37 Celsius saline.

P8023 Niewiadomska,G., Baksalerska-Pazera,M., Gasiorowska,A., Mietelska,A. Nerve growth factor differentially affects spatial and recognition memory in aged rats. Neurochemical Research 2006; 31(-12-):1481-1490. >>> Nerve growth factor, beta; CSF, artificial; CSF/CNS; Rat; 2004; 1 month; ALZET brain infusion kit used; post op. care (antibiotic and analgesic to scalp + fluid therapy); silastic tubing used; 28 G cannula.

P7365 Jakubowska-Dogru,E., Guemuesbas,U. Chronic intracerebroventricular NGF administration improves working memory in young adult memory deficient rats. Neuroscience Letters 2005; 382(-1-2-):45-50. >>> Nerve growth factor, recomb. rat; CSF, artificial; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; memory; behavioral study.

P7244 Obata,K., Katsura,H., Mizushima,T., Yamanaka,H., Kobayashi,K., Dai,Y., Fukuoka,T., Tokunaga,A., Tominaga,M., Noguchi,K. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury. Journal of Clinical Investigation 2005; 115(-9-):2393-2401. >>> SB203580; antibody, anti-nerve growth factor; oligodeoxynucleotide, antisense; oligodeoxynucleotide, FITC antisense; oligodeoxynucleotide, missense; DMSO; saline; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle, or sham surgery or mismatch ODN or contralateral no treatment; antisense (TRPA1); brain tissue distribution; 50% DMSO.

P7289 Pereira,P.A., Cardoso,A., Paula-Barbosa,M.M. Nerve growth factor restores the expression of vasopressin and vasoactive intestinal polypeptide in the suprachiasmatic nucleus of aged rats. Brain Research 2005; 1048(-1-2-):123-130. >>> Nerve growth factor; dye, methylene blue; CSF/CNS; Rat; 2002; 14 days; Functionality of mp verified by pretesting delivery rate; ALZET brain infusion kit used; peptides; post op. care (SC saline, local antiseptic); mp filled with Methylene blue, air-oil spacer at tubing connection, tubing filled with NGF; Lynch coil; "Because there is evidence that the surgical procedure does not interfere with the structure of SCN, vehicle - treated rats were not included in this study." (p. 124).

P6996 Bruno,M.A., Clarke,P.B.S., Seltzer,A., Quirion,R.M., Burgess,K., Cuello,A.C., Saragovi,H.U. Long-lasting rescue of age-associated deficits in cognition and the CNS cholinergic phenotype by a partial agonist peptidomimetic ligand of TrkA. Journal of Neuroscience 2004; 24(-37-):8009-8018. >>> Nerve growth factor; D3; dye, methylene blue; CSF, artificial, buffered; saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by infusion volume (pg. 8011); no stress (see pg. 8012); ALZET brain infusion kit used; peptides; used air-oil spacer in PE tubing at pump end, PE tubing used in coil for D3 mp filled with dye; D3 is a peptidomimetic which binds TrKA.

P6922 Chen,J.R., Wang,Y.J., Tseng,G.F. The effects of decompression and exogenous NGF on compressed cerebral cortex. Journal of Neurotrauma 2004; 21(-11-):1640-1651. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Rat; 2ML2; 14 days; Used coiled PE tubing (Lynch coil).

P7008 DeKosky,S.T., Abrahamson,E.E., Taffe,K.M., Dixon,C.E., Kochanek,P.M., Ikonomovic,M.D. Effects of post-injury hypothermia and nerve growth factor infusion on antioxidant enzyme activity in the rat: implications for clinical therapies. Journal of Neurochemistry 2004; 90(-4-):998-1004. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Rat; 2002; 1,3 days; Controls received mp w/ vehicle; stability verified by increased ChAT activity.

P6354 Harrington,A.W., Leiner,B., Blechschmitt,C., Arevalo,J.C., Lee,R., Moerl,L., Meyer,M., Hempstead,B.L., Yoon,S.O., Giehl,K.M. Secreted proNGF is a pathophysiological death-inducing ligand after adult CNS injury. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-16-):6226-6230. >>> Antibody, anti-nerve growth factor, neutralizing mouse; serum; immunoglobulin-G; penicillin; streptomycin; PBS; CSF/CNS (cortex); Rat; 2001; 1 week; Controls received mp w/ vehicle.

P6451 Seki,S., Sasaki,K., Igawa,Y., Nishizawa,O., Chancellor,M.B., De Groat,W.C., Yoshimura,N. Suppression of detrusor-sphincter dyssynergia by immunoneutralization of nerve growth factor in lumbosacral spinal cord in spinal cord injured rats. Journal of Urology 2004; 171(-1-):478-482. >>> Antibody, anti-nerve growth factor; CSF, artificial; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; spinal cord injury; PE-10 used for catheterization.

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“However, the s.c. mini-osmotic pump delivery of recombinant leptin raised circulating leptin into the physiological range (Table 2). These data confirmed the effectiveness of the s.c. infusion of leptin at 0.1 mg/day in restoring serum leptin levels.” Zhang et al. Journal of Endocrinology 2002; 174:455-461.

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References on the Administration of Neurotrophic Factors
Using ALZET^® Osmotic Pumps

1. Brain-derived Neurotrophic Factor

2. Ciliary Neurotrophic Factor

3. Glial-derived Neurotrophic Factor

4. NT-3

5. NT-4-5

6. Nerve Growth Factor

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Researchers are saying ...

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ALZET® Osmotic Pumps - Research Applications.

References on the Administration of Neurotrophic Factors Using ALZET® Osmotic Pumps

1. Brain-derived Neurotrophic Factor

2. Ciliary Neurotrophic Factor

3. Glial-derived Neurotrophic Factor

4. NT-3

5. NT-4-5

6. Nerve Growth Factor

Looking for references? We can help!

Researchers are saying ...

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References on the Administration of Neurotrophic Factors
Using ALZET^® Osmotic Pumps