Recent References on the Study of Obesity, Appetite & Satiety
Using ALZET® Osmotic Pumps
1. Agouti-Related Peptide
P9007 Hillebrand,J.J.G., Kas,M.J.H., Scheurink,A.J.W., van Dijk,G., Adan,R.A.H. AgRP(83-132) and SHU9119 differently affect activity-based anorexia. European Neuropsychopharmacology 2006; 16(-6-):403-412. >>> SHU 9119; agouti-related protein (83-132), human; Saline, sterile, isotonic; CSF/CNS; Rat; 1007D; 4, 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; post op. care (Buprenorphine, temagesic); animal info (female, Wistar wu); melanocortin antagonist.
P8331 Joppa,M.A., Gogas,K.R., Foster,A.C., Markison,S. Central infusion of the melanocortin receptor antagonist agouti-related peptide (AgRP(83-132)) prevents cachexia-related symptoms induced by radiation and colon-26 tumors in mice. Peptides 2007; 28(-3-):636-642. >>> Agouti-related peptide (83-132); Saline, sterile; CSF/CNS; Mice; 1003D; 72 hours; Controls received mp w/ vehicle; cancer (colon adenocarcinoma); peptides; animal info (male, BALB/C, 6 weeks old, 22 grams); Research Diets D12689B.
P5827 Kas,M.J.H., van Dijk,G., Scheurink,A.J.W., Adan,R.A.H. Agouti-related protein prevents self-starvation. Molecular Psychiatry 2003; 8(-2-):235-240. >>> Agouti-related protein; Saline; CSF/CNS; Rat; 1007D; 4-5 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; peptides; catheter tubing filled w/ saline to allow 2-3 day delayed infusion; Agouti-related protein is a suppressor of melanocortin receptor activity.
P5583 Fekete,C., Sarkar,S., Rand,W.M., Harney,J.W., Emerson,C.H., Bianco,A.C., Lechan,R.M. Agouti-related protein (AGRP) has a central inhibitory action on the hypothalamic-pituitary-thyroid (HPT) axis; Comparisons between the effect of AGRP and neuropeptide Y on energy homeostasis and the HPT axis. Endocrinology 2002; 143(-10-):3846-3853. >>> Neuropeptide Y; Agouti-related protein; CSF, artificial; CSF/CNS; rat; 1003D; 3 days; peptides; cannula was implanted then occluded with a dummy cannula for one week prior to infusion to allow for recovery; AGRP is an appetite stimulant.
2. CART (55-102)
P7017 Choi,Y.H., la-Fera,M.A., Li,C.L., Hartzell,D.L., Little,D.E., Kuhar,M.J., Balle,C.A. CART peptide: central mediator of leptin-induced adipose tissue apoptosis?. REGULATORY PEPTIDES 2004; 121(-1-3-):155-162. >>> CART (55-102); leptin, recomb. rat; CSF, artificial; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ vehicle; dose-response; peptides; mp primed overnight; catheter connecting mp to cannula filled with 12 ul aCSF to give 24 hours lag time before agent delivery.
P5040 Rohner-Jeanrenaud,F., Craft,L.S., Bridwell,J., Suter,T.M., Tinsley,F.C., Smiley,D.L., Burkhart,D.R., Statnick,M.A., Heiman,M.L., Ravussin,E., Caro,J.F. Chronic central infusion of cocaine- and amphetamine-regulated transcript (CART 55-102): effects on body weight homeostasis in lean and high -fat-fed obese rats. International Journal of Obesity 2002; 26(--):143-149. >>> CART (55-102); Saline; ascorbic acid;; CSF/CNS; rat; 2001; 6 days; controls received mp w/ vehicle; in vitro stability verified by HPLC: 72% of agent was stable after 7 days (p. 145); peptides; ALZET Brain Infusion Kit used; CART is Cocaine-and Amphetamine-Regulated Transcript; pumps primed for at least 20 hours (p. 144).
3. Corticotropin-rel. Factor
P7604 Bernier,N.J., Craig,P.M. CRF-related peptides contribute to stress response and regulation of appetite in hypoxic rainbow trout. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2005; 289(-4-):R982-R990. >>> Corticotropin-rel. factor, a helical (9-41); Saline, physiological; NaOH; CSF/CNS; Fish (rainbow trout); 1003D; 8 days; Controls received mp w/ vehicle; no stress (see pg. R984); peptides; animal info (male, female, hypoxia); CRF receptor antagonist; x-ray radiography; mp encased in a layer of dialysis tubing; cannula placement confirm; mp at 14 C.
P6607 Kagamiishi,Y., Yamamoto,T., Watanabe,S. Detrimental role of corticotropin-releasing factor on the decrease of CA1 field potential induced by in vitro ischemia in rat hippocampal slices. JOURNAL OF PHARMACOLOGICAL SCIENCES 2004; 94(-1-):39-44. >>> Corticotropin-rel. factor; astressin; Saline; BSA; ascorbic acid; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; ALZET brain infusion kit 1 used; 2 week recovery period by filling tubing and cannula with sterile saline; tubing attached to pump after recovery periord; astressin is a novel CRF antagonist; BSA and ascorbic acid was 0.1%; ischemia (cerebral).
P3228 Jain,V., Shi,S.-Q., Vedernikov,Y.P., Saade,G.R., Chwalisz,K., Garfield,R.E. In vivo effects of corticotropin-releasing factor in pregnant rats. Am.J.Obstet.Gynecol. 1998; 178(--):186-191. >>> Corticotropin-rel. factor; Corticotropin-rel. factor, 9-41; Saline, normal; SC; rat (pregnant); 2ML1; no duration posted; controls received mp w/vehicle; peptides; cardiovascular.
P3927 Buwalda,B., de Boer,S.F., Van Kalkeren,A.A., Koolhaas,J.M. Physiological and behavioral effects of chronic intracerebroventricular infusion of corticotropin-releasing factor in the rat. Psychoneuroendocrinology 1997; 22(-5-):297-309. >>> Corticotropin-rel. factor, ovine; Saline; BSA; Ascorbic acid; CSF/CNS; rat; 2002; 10 days; controls received mp w/ vehicle; peptides; ALZET brain infusion kit used; catheter filled w/ saline to delay agent delivery.
P3832 Huang,T-S. Concomitant infusion of ovine corticotropin-releasing hormone does not prevent suppression of the hypothalamus-pituitary-adrenal axis by dexamethasone in male rats. J.Endocrinol.Invest. 1997; 20(--):393-396. >>> Dexamethasone; Corticotropin-rel. factor, ovine; SC; rat; 1003D; 2001; 3,7 days; replacement therapy; peptides.
P4268 Heinrichs,S.C., Lapsansky,J., Behan,D.P., Chan,R.K.W., Sawchenko,P.E., Lorang,M., Ling,N., Vale,W.W., De Souza,E.B. Corticotropin-releasing factor-binding protein ligand inhibitor blunts excessive weight gain in genetically obese Zucker rats and rats during nicotine withdrawal. Proc.Natl.Acad.Sci.USA 1996/12; 93(--):15475-15480. >>> Nicotine tartrate salt; Corticotropin-rel. factor, 6-33;; Saline; SC; CSF/CNS;; rat; 2001; 2002;; 14 days; controls received mp w/ vehicle; functionality of mp verified by plasma levels; peptides; ALZET brain infusion kit used; recomb. human corticotropin releasing factor used; dummy cannula maintained cannula patency during one week recovery period; after 14 day SC nicotine infusion, r/h CRF (6-33) was infused ICV.
P2719 Labeur,M.S., Arzt,E., Wiegers,G.J., Holsboer,F., Reul,J.M.H.M. Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression. Endocrinology 1995; 136(-6-):2678-2688. >>> Corticotropin-rel. factor; Saline, sterile; Ascorbic acid; CSF/CNS; rat; 2001; 1 week; controls received mp w/ vehicle; replacement therapy (adrenalectomy); peptides.
P3197 Hauger,R.L., Aguilera,G. Regulation of pituitary corticotropin releasing hormone (CRH) receptors by CRH: interaction with vasopressin. Endocrinology 1993; 133(--):1708-1714. >>> Corticotropin-rel. factor; Vasopressin; Vasopressin antagonist; SC; rat; 2001; 48 hours; controls received inactive mp or silastic tubing; peptides; the vasopressin antagonist was (mercapto cyclopenta-methylene propionic acid)-[methyl-tyrosine]arginine VP; agents given singly or together.
P2287 Rivest,S., Plotsky,P.M., Rivier,C. CRF alters the infundibular LHRH secretory system from the medial preoptic area of female rats: possible involvement of opioid receptors. Neuroendocrinology 1993; 57(--):236-246. >>> Corticotropin-rel. factor; Ascorbic acid; Albumin, bovine serum; Saline; CSF/CNS (hypothalamic medial preoptic area); rat; 2001; 7 days; two pumps connected to double internal cannula; animals were ovariectomized.
4. Dexfenfluramine
P7822 Callebert,J., Esteve,J.M., Herve,P., Peoc'h,K., Tournois,C., Drouet,L., Launay,J.M., Maroteaux,L. Evidence for a control of plasma serotonin levels by 5-hydroxytryptamine2B receptors in mice. Journal of Pharmacology and Experimental Therapeutics 2006; 317(-2-):724-731. >>> Dexfenfluramine; RS-127445; Mice; ; Controls received mp w/ vehicle; comparison of IP injections vs. mp; cardiovascular; animal info (129PAS wt or 5-HT2B-R(-/-), 6wk old, 20-25g, male, female).
P5548 Launay,J.M., Herve,P., Peoc'h,K., Tournois,C., Callebert,J., Nebigil,C.G., Etienne,N., Drouet,L., Humbert,M., Simonneau,G., Maroteaux,L. Function of the serotonin 5-hydroxytryptamine 2B receptor in pulmonary hypertension. Nature Medicine 2002; 8(-10-):1129-1135. >>> RS-127445; dexfenfluramine; Mice; ; Controls received mp w/ vehicle; RS-127445 is a highly selective, high affinity 5-HT2B receptor antagonist; dexfenfluramine is an amphetamine-derived appetite suppressant.
P4569 Bratter,J, Gessner,I.H, Rowland,N.E. Effects of prenatal co-administration of phentermine and dexfenfluramine in rats.. European Journal of Pharmacology 1999; 369(--):R1-R3. >>> Phentermine; dexfenfluramine;; water;; SC;; rat (pregnant);; 2002;; 14 days;; controls received mp w/vehicle; teratology; phentermine and dexfenfluramine infused together in the same pump;.
5. Ghrelin
P8993 Wu,R.Q., Dong,W.F., Cui,X.X., Zhou,M., Simms,H.H., Ravikumar,T.S., Wang,P. Ghrelin down-regulates proinflammatory cytokines in sepsis through activation of the vagus nerve. Annals of Surgery 2007; 245(-3-):480-486. >>> Ghrelin, rat; Saline, normal; IV (jugular); Rat; 2001D; 15 hours; Controls received mp w/ vehicle; half-life (p. 485) 11-17 minutes (rat); peptides; animal info (male, Sprague Dawley, 275-325 g.); Sepsis by cecal ligation and puncture; vagotomy; "ghrelin's half-life is 11 minutes in the normal rat and 17 minutes at the late stage of sepsis. Therefore, a continuous infusion of ghrelin is required to maintain its effective concentration in the circulation." (p. 485).
P8949 Strassburg,S., Anker,S.D., Castaneda,T.R., Burget,L., Perez-Tilve,D., Pfluger,P.T., Nogueiras,R., Halem,H., Dong,J.Z., Culler,M.D., Datta,R., Tschoep,M.H. Long-term effects of ghrelin and ghrelin receptor agonists on energy balance in rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 295(-1-):E78-E84. >>> BIM-28131; BIM-28125; ghrelin; Sodium chloride; Tween 80; serum, rat; SC; Rat; 2002; 4 weeks; Dose-response (pg. E80, fig. 1); comparison of SC injections vs. mp; pumps replaced on day 15; half-life (p. E83) ghrelin is "~10-30" min; peptide; post op. care (buprenorphine); animal info (male, Sprague Dawley, 297-316 g.); BIM-28125 and BIM-28131 are GHS-R agonists (growth hormone secretagogue receptor); pumps were primed overnight.
P8842 DeBoer,M.D., Zhu,X.X., Levasseur,P.R., Inui,A., Hu,Z.Y., Han,G.F., Mitch,W.E., Taylor,J.E., Halem,H.A., Dong,J.Z., Datta,R., Culler,M.D., Marks,D.L. Ghrelin treatment of chronic kidney disease: Improvements in lean body mass and cytokine profile. Endocrinology 2008; 149(-2-):827-835. >>> Ghrelin, human; BIM-28131; BIM-28125; Saline; serum, inactivated rate; Tween 80; SC; Rat; 2002; 14 days; Controls received mp w/ saline; replacement therapy (Nephrectomy 5/6); half-life (pg. 834) "short"; peptides; animal info (male, F344/NTacfBR, 130-150g.); endocrinology; synthetic receptor agonists.
P8625 Dixit,V.D., Yang,H.W., Sun,Y.X., Weeraratna,A.T., Youm,Y.H., Smith,R.G., Taub,D.D. Ghrelin promotes thymopoiesis during aging. Journal of Clinical Investigation 2007; 117(-10-):2778-2790. >>> Ghrelin; leptin, recomb. mouse; PBS; SC; Mice; 2 weeks; Controls received mp w/ vehicle; peptides; animal info (Balb/c; 2,4,14,20 and 24 months old, femail, C57BL/6, 2,68,18 months old); endocrinology.
P8013 Nakazato,M., Murakami,N., Date,Y., Kojima,M., Matsuo,H., Kangawa,K., Matsukura,S. A role for ghrelin in the central regulation of feeding. Nature 2001/1/11; 409(-6817-):194-198. >>> Ghrelin; CSF/CNS; Rat; 12 days; Peptides.
P8001 Tschop,M., Smiley,D.L., Heiman,M.L. Ghrelin induces adiposity in rodents. Nature 2000/10/19; 407(-6806-):908-913. >>> Ghrelin; CSF/CNS; 2001; 7 days; Controls received mp w/ water; peptides; animal info (male, Sprague-Dawley).
P7896 Diano,S., Farr,S.A., Benoit,S.C., McNay,E.C., da Silva,I., Horvath,B., Gaskin,F.S., Nonaka,N., Jaeger,L.B., Banks,W.A., Morley,J.E., Pinto,S., Sherwin,R.S., Xu,L., Yamada,K.A., Sleeman,M.W., Tschoep,M.H., Horvath,T.L. Ghrelin controls hippocampal spine synapse density and memory performance. Nature Neuroscience 2006; 9(-3-):381-388. >>> Ghrelin; Saline; SC; Mice; 14 days; Controls received mp w/ vehicle; replacement therapy (ghrelin knockout); peptides; animal info (C57/B16, Ghsr -/-, male); neurodegenerative (Alzheimer's disease).
P7837 Martini,A.C., Fernandez-Fernandez,R., Tovar,S., Navarro,V.M., Vigo,E., Vazquez,M.J., Davies,J.S., Thompson,N.M., Aguilar,E., Pinilla,L., Wells,T., Dieguez,C., Tena-Sempere,M. Comparative analysis of the effects of ghrelin and unacylated ghrelin on luteinizing hormone secretion in male rats. Endocrinology 2006; 147(-5-):2374-2382. >>> Ghrelin, rat; ghrelin, des-octanoyl; L163, 255; Saline, sterile; heparin; BSA; IV (jugalar); Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, Sprague-Dawley, 12 wk. old); L163, 255 is GHS-R1a-specific agonist.
P7609 Wu,R.Q., Dong,W.F., Zhou,M., Cui,X.X., Simms,H.H., Wang,P. Ghrelin improves tissue perfusion in severe sepsis via downregulation of endothelin-1. Cardiovascular Research 2005; 68(-2-):318-326. >>> Ghrelin, rat; PD 142893; Saline, normal; IV (jugular); Rat; 2001D; 15 hours; Controls received mp w/ vehicle or sham operated; functionality of mp verified by plasma levels; half-life (pg. 324) 11-17 min. in rat; cardiovascular; peptides; animal info (male, Sprague-Dawley 275-325 g); cecal ligation.
P7303 Riley,L.G., Fox,B.K., Kaiya,H., Hirano,T., Grau,E.G. Long-term treatment of ghrelin stimulates feeding, fat deposition, and alters the GH/IGF-I axis in the tilapia, Oreochromis mossambicus. General and Comparative Endocrinology 2005; 142(-1-2-):234-240. >>> Ghrelin-C8, tilapia; ghrelin-C10, tilapia; Saline, sterile; IP; Fish (tilapia); 1002; 21 days; Controls received mp w/ vehicle; no stress (see pg. 235); peptides; post op. care (maracyn); fish temperature of 25 degrees celsius gives a 28 day predicted duration for mp 1002; endocrinology.
P6948 Kim,M.S., Namkoong,C., Kim,H.S., Jang,P.G., Pak,Y.M.K., Katakami,H., Park,J.Y., Lee,K.U. Chronic central administration of ghrelin reverses the effects of leptin. International Journal of Obesity 2004; 28(-10-):1264-1271. >>> Leptin; ghrelin; Saline; CSF/CNS (third ventricle); Rat; 7 days; Controls received mp w/ vehicle; plasma levels; peptides; Plastics One cannula used; 7 day recovery from cannulation prior to pump implant; cannulation verified by dipsogenic response to ICV administration of angiotensin II (150 ng/rat), positive response was defined as the consumption of 5 ml of water within 1 hr of injection.
P6836 Ariyasu,H., Takaya,K., Iwakura,H., Hosoda,H., Akamizu,T., Arai,Y., Kangawa,K., Nakao,K. Transgenic mice overexpressing des-acyl ghrelin show small phenotype. Endocrinology 2005; 146(-1-):355-364. >>> Ghrelin, rat des-acyl; Saline; IP; Mice; 1002; 10 days; Controls received mp w/ vehicle; no stress (see pg. 360); peptides.
P6549 Thompson,N.M., Gill,D.A.S., Davies,R., Loveridge,N., Houston,P.A., Robinson,I.C.A.F., Wells,T. Ghrelin and des-octanoyl ghrelin promote adipogenesis directly in vivo by a mechanism independent of the type 1a growth hormone secretagogue receptor. Endocrinology 2004; 145(-1-):234-242. >>> Ghrelin, rat; ghrelin, des-octanoyl rat; L-163,255; Saline, sterile; BSA; heparin; Bone (tibial bone marrow); Rat; 2001; 7 days; Controls received mp w/ vehicle; no stress (see pg.236); peptides; cyanoacrylate adhesive; L-163,255 is a spiropiperidine GHS-R1a agonist; 5 U/ml of heparin.
P5828 Tschop,M., Smiley,D.L., Heiman,M.L. Ghrelin induces adiposity in rodents. Nature 2003; 407(-6806-):908-913. >>> Ghrelin; Water; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig 4a; pg 911); peptides; 1 ul/hr pump model for 7 days; obesity research.
P4690 Date,Y, Murakami,N, Kojima,M, Kuroiwa,T, Matsukura,S, Kangawa,K., Nakazato,M. Central effects of a novel acylated peptide, ghrelin, on growth hormone release in rats.. Biochemical and Biophysical Research Communications 2000; 275(--):477-480. >>> Ghrelin; Saline; CSF/CNS; rat; 2002; 12 days; Controls received mp w/ vehicle; comparison of single bolus ICV injections vs. mp; peptides; Ghrelin is a novel growth-hormone-releasing acylated peptide; proper cannulae placement verified at end of experiment by the administration of dye..
6. Glucagon-like Peptide-1
P6648 Nikolaidis,L.A., Hentosz,T., Stolarski,C., Elahi,D., Shannon,R.P. Recombinant glucagon like peptide-1 (rGLP-1) improves survival in spontaneously hypertensive heart failure (SHHF) rats. Circulation 2003; 108(-17-):93-94. >>> Glucagon-like peptide-1 (7-36) amide; Saline; SC; Rat; ; Controls received mp w/ vehicle.
R0153 Meier,J.J., Gallwitz,B., Schmidt,W.E., Nauck,M.A. Glucagon-like peptide 1 as a regulator of food intake and body weight: therapeutic perspectives. European Journal of Pharmacology 2002; 440(--):269-279. >>> Glucagon-like peptide-1; CSF/CNS; mice; ; continuous ICV infusion via minipump mentioned on p. 272; numerous delivery methods; brain tissue distribution; peptides.
P6185 Farilla,L., Hui,H.X., Bertolotto,C., Kang,E., Bulotta,A., Di Mario,U., Perfetti,R. Glucagon-like peptide-1 promotes islet cell growth and inhibits apoptosis in Zucker diabetic rats. Endocrinology 2002; 143(-11-):4397-4408. >>> Glucagon-like peptide-1; Saline; SC; Rat; 2 days; Controls received mp w/ vehicle; peptides.
P5478 Hui,H.X., Farilla,L., Merkel,P., Perfetti,R. The short half-life of glucagon-like peptide-1 in plasma does not reflect its long-lasting beneficial effects. European Journal of Endocrinology 2002; 146(-6-):863-869. >>> Glucagon-like peptide-1; SC; Rat; 2 days; Dose-respose (figure 2, p. 146); half-life (p. 863) 1.5 - 5 min; peptides; agent is human recombinant.
P5320 Perry,T., Haughey,N.J., Mattson,M.P., Egan,J.M., Greig,N.H. Protection and reversal of excitotoxic neuronal damage by glucagon-like peptide-1 and exendin-4. Journal of Pharmacology and Experimental Therapeutics 2002; 302(-3-):881-888. >>> Glucagon-like peptide-1; Exendin-4; CSF, artificial; CSF/CNS; Rat; 1002; 14 days; Controls received mp w/ vehicle; Exendin-4 half-life (p. 882) 120 mins; peptides; ALZET brain infusion kit used.
P4217 Donahey,J.C.K., van Dijk,G., Woods,S.C., Seeley,R.J. Intraventricular GLP-1 reduces short- but not long-term food intake or body weight in lean and obese rats. Brain Research 1998; 779(--):75-83. >>> Glucagon-like peptide-1(7-36) amide; Saline; CSF/CNS (third ventricle); rat; 2002; 4 days; controls received mp w/saline; functionality of mp verified by movement of air bubble along tubing; one week recovery after cannula placement; cannula placement confirmed by administration of angiotensin II; pump filled with saline and agent and loaded in catheter tubing; air bubble used to separate solutions; Lynch coil technique; peptides.
P4482 Wang,Y, Perfetti,R, Greig,N.H, Hooloway,H.W, Deore,K.A, Montrose-Rafizadeh,C, Elahi,D, Egan,J.M. Glucagon-like peptide-1 can reverse the age-related decline in glucose tolerance in rats.. The Journal of Clinical Investigation 1997/6; 99(-12-):2883-2889. >>> Glucagon-like peptide-1 (GLP-1); Saline;; SC;; rat;; 48 hours;; controls received mp w/vehicle; functionality of mp verified by plasma GLP-1 levels; peptides;.
P3837 Mizuno,A., Kuwajima,M., Ishida,K., Noma,Y., Murakami,T., Tateishi,K., Sato,I., Shima,K. Extrapancreatic action of truncated glucagon-like peptide-1 in otsuka long-evans tokushima fatty rats, an animal model for non-insulin-dependent diabetes mellitus. Metabolism 1997; 46(-7-):745-749. >>> Glucagon-like peptide-1 (7-36) amide; SC; rat; 2002; 4 weeks; controls received mp w/vehicle; functionality of mp verified by plasma GLP-1 assays at days 0 and 12; peptides.
P3172 Hargrove,D.M., Nardone,N.A., Persson,L.M., Parker,J.C., Stevenson,R.W. Glucose-dependent action of glucagon-like peptide-1 (7-37) in vivo during short- or long- term administration. Metabolism 1995; 44(-9-):1231-1237. >>> Glucagon-like peptide-1 (7-37); Tween 80; Saline; SC; rat; 5 days; controls received mp with vehicle; comparison of acute infusion vs. mp; peptides; "... it is likely that only a portion of the GLP-1(7-37) administered subcutaneously was absorbed..." (p. 1235).
P3287 Kawai,K., Ohashi,S. Long-term (1-month) administration of GLP-1(7-36)amide to normal and diabetic rats. Digestion 1993; 54(-6-):359-360. >>> Glucagon-like peptide-1(7-36) amide; SC; rat; 2ML4; 4 weeks; controls received sham operation; peptides.
7. Leptin (2006-2009)
P9179 Zhang,R., Dhillon,H., Yin,H., Yoshimura,A., Lowell,B.B., Maratos-Flier,E., Flier,J.S. Selective Inactivation of Socs3 in SF1 Neurons Improves Glucose Homeostasis without Affecting Body Weight. Endocrinology 2008; 149(-11-):5654-5661. >>> Leptin, murine; SC; Mice; 1002; 10 days; Controls received mp w/ saline; peptides; animal info (male, C57BL/6, 20 wks old, Socs3 flox/flox, Sf1-Cre, wt).
P9174 Trotter-Mayo,R.N., Roberts,M.R. Leptin acts in the periphery to protect thymocytes from glucocorticoid-mediated apoptosis in the absence of weight loss. Endocrinology 2008; 149(-10-):5209-5218. >>> Leptin; IP; CSF/CNS; Mice; 1002; 12 days; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ob/ob, db/db, wt, C57BL/6).
P9173 Trevaskis,J.L., Coffey,T., Cole,R., Lei,C., Wittmer,C., Walsh,B., Weyer,C., Koda,J., Baron,A.D., Parkes,D.G., Roth,J.D. Amylin-Mediated Restoration of Leptin Responsiveness in Diet-Induced Obesity: Magnitude and Mechanisms. Endocrinology 2008; 149(-11-):5679-5687. >>> Leptin, murine; amylin, rat; Water, sterile; DMSO; Rat; 4 weeks; Controls received mp w/ vehicle; multiple pumps per animal (2); animal info (DIO-prone, Sprague Dawley); 50% DMSO used; Research Diets D1226B.
P9131 Chiba,T., Shinozaki,S., Nakazawa,T., Kawakami,A., Ai,M., Kaneko,E., Kitagawa,M., Kondo,K., Chait,A., Shimokado,K. Leptin deficiency suppresses progression of atherosclerosis in apoE-deficient mice. Atherosclerosis 2008; 196(-1-):68-75. >>> Leptin, recomb. mouse; SC; Mice; 2004; ; Controls received mp w/ saline; peptides; animal info (C57BL/6, ApoE-/-); Research Diets D12108.
P9042 Gutman,R., Hacmon-Keren,R., Choshniak,I., Kronfeld-Schor,N. Effect of food availability and leptin on the physiology and hypothalamic gene expression of the golden spiny mouse: a desert rodent that does not hoard food. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-6-):R2015-R2023. >>> Leptin; SC; Mice; 2002; 23 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; post op. care (Baytril); animal info (golden, spiny, male).
P9025 Unniappan,S., Kieffer,T.J. Leptin extends the anorectic effects of chronic PYY(3-36) administration in ad libitum-fed rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-1-):R51-R58. >>> Peptide YY (3-36), rat; peptide YY (3-36), human; leptin; Saline; SC; Rat; 2001D; 2ML1; 2ML2; 7 days; 24 hours; Controls received mp w/ vehicle; animal info (Fischer 344, 224 g.); 14-day pumps were removed after 7 days, and reimplanted into a new set of animals; "In the coadministration studies, a single pump was used to deliver both PYY (3-36) and leptin." pg. R53; "PYY (3-36) remained biologically active while in the pumps for at least 9 days.".
P9011 Covey,S.D., Wideman,R.D., McDonald,C., Unniappan,S., Huynh,F., Asadi,A., Speck,M., Webber,T., Chua,S.C., Kieffer,T.J. The pancreatic beta cell is a key site for mediating the effects of leptin on glucose homeostasis. Cell Metabolism 2006; 4(-4-):291-302. >>> Leptin; PBS; SC; Mice; mice (transgenic); 2001D; 12 hours; Controls received mp w/ saline; peptides; animal info (C57BL/6-TgN (R/Pcre) 25 Mgn, Lepr flox/flox, 10 wks old).
P8995 Leclercq,I.A., Vansteenberghe,M., Lebrun,V.B., VanHul,N.K., barca-Quinones,J., Sempoux,C.L., Picard,C., Staerkel,P., Horsmans,Y.L. Defective hepatic regeneration after partial hepatectomy in leptin-deficient mice is not rescued by exogenous leptin. LABORATORY INVESTIGATION 2006; 86(-11-):1161-1171. >>> Leptin, recomb. mouse; SC; Mice; 1003D; 44 hours; Controls received no treatment; functionality of mp verified by serum leptin concentrations; replacement therapy (partial hepatectomy); comparison of IP injections vs. mp; peptides; animal info (female, ob/ob, lean).
P8941 Komatsu,T., Chiba,T., Yamaza,H., To,K., Toyama,H., Higami,Y., Shimokawa,I. Effect of leptin on hypothalamic gene expression in calorie-restricted rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 2006; 61(-9-):890-898. >>> Leptin, recomb.; PBS; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Fischer 344, 34 wks old).
P8915 Lujan,M.E., Krzemien,A.A., Reid,R.L., Van Vugt,D.A. Effect of leptin administration on ovulation in food-restricted rhesus monkeys. Neuroendocrinology 2006; 84(-2-):103-114. >>> Leptin, recomb. human; Water, sterile; CSF/CNS; Monkey; 2002; 32 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 2 wks; stress/adverse reaction: (see pg. 104) 1 animal removed from study due to CNS infection at 28 weeks; peptides; animal info (female, Rhesus, 6-10 yrs. old, 5-12 kg.); endocrinology.
P8910 do Carmo,J.M., Hall,J.E., da Silva,A.A. Chronic central leptin infusion restores cardiac sympathetic-vagal balance and baroreflex sensitivity in diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-5-):H1974-H1981. >>> Leptin; CSF/CNS; Rat; 1007D; 7 days; Peptides; animal info (male, Sprague Dawley, 340-360 g.); tygon tubing used; diabetes.
P8902 Judge,M.K., Zhang,J., Tuemer,N., Carter,C., Daniels,M.J., Scarpace,P.J. Prolonged hyperphagia with high-fat feeding contributes to exacerbated weight gain in rats with adult-onset obesity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-3-):R773-R780. >>> Leptin, recomb. murine; SC; Rat; 2001; 7 days; Controls received mp w/ saline; dose-response (fig. 8); peptides; animal info (male, F344 x BN); obesity.
P8891 Duan,J., Choi,Y.H., Hartzell,D., la-Fera,M.A., Hamrick,M., Baile,C.A. Effects of subcutaneous leptin injections on hypothalamic gene profiles in lean and ob/ob mice. Obesity 2007; 15(-11-):2624-2633. >>> Leptin, recomb. mouse; SC; Mice; 1002; 14 days; Controls received mp w/ PBS; peptides; animal info (obese, ob/ob, lean).
P8869 Sennello,J.A., Fayad,R., Pini,M., Gove,M.E., Ponemone,V., Cabay,R.J., Siegmund,B., Dinarello,C.A., Fantuzzi,G. Interleukin-18, together with interleukin-12, induces severe acute pancreatitis in obese but not in nonobese leptin-deficient mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-23-):8085-8090. >>> Leptin; SC; Mice; 1 month; Replacement therapy (leptin deficient); peptides; animal info (ob/ob, slim, female, 6-10 wks old); obesity.
P8866 Terao,S., Yilmaz,G., Stokes,K.Y., Ishikawa,M., Kawase,T., Granger,D.N. Inflammatory and injury responses to ischemic stroke in obese mice. Stroke 2008; 39(-3-):943-950. >>> Leptin, mouse; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma leptin levels; peptides; post op. care (neomycin trisulfate salt hydrate); animal info (young, 5-7 wks old, ob/ob); obesity.
P8823 Cariou,B., Bouchaert,E., Abdelkarim,M., Dumont,J., Caron,S., Fruchart,J.C., Burcelin,R., Kuipers,F., Staels,B. FXR-deficiency confers increased susceptibility to torpor. FEBS Letters 2007; 581(-27-):5191-5198. >>> Leptin, recomb. mouse; SC; 1003D; 3 days; Controls received mp w/ sterile saline; functionality of mp verified by plasma leptin levels; peptides; animal info (female, C57BL/6, wt, FXR -/-).
P8807 Sato,S., Hanada,R., Kimura,A., Abe,T., Matsumoto,T., Iwasaki,M., Inose,H., Ida,T., Mieda,M., Takeuchi,Y., Fukumoto,S., Fujita,T., Kato,S., Kangawa,K., Kojima,M., Shinomiya,K., Takeda,S. Central control of bone remodeling by neuromedin U. Nature Medicine 2007; 13(-10-):1234-1240. >>> Neuromedin U-23, rat; leptin, human; CSF/CNS; Mice; 28 days; Controls received mp w/ PBS; peptides; animal info (C57BL/6J, wt, LepOb, Nmu -/-, Adrb2 -/-, male, female, 3 months old).
P8800 Park,S., Hong,S.M., Sung,S.R., Jung,H.K. Long-term effects of central leptin and resistin on body weight, insulin resistance, and beta-cell function and mass by the modulation of hypothalamic leptin and insulin signaling. Endocrinology 2008; 149(-2-):445-454. >>> Leptin, recomb. rat; resistin, recomb. murine; CSF, artificial; CSF/CNS; Rat; 4 weeks; Controls received mp w/ vehicle; replacement therapy (pancreatectomy, 90%); peptides; animal info (male, Sprague Dawley, 220g).
P8798 Trivedi,P., Yang,R.H., Barouch,L.A. Decreased p110-alpha catalytic activity accompanies increased myocyte apoptosis and cardiac hypertrophy in leptin deficient ob/ob mice. CELL CYCLE 2008; 7(-5-):560-565. >>> Leptin, recomb. mouse; SC; Mice; 1002; 4 weeks; Replacement therapy (leptin-deficient mice); pumps replaced after 14 days; cardiovascular; peptides; animal info (ob/ob, db/db, wt, 2-3 months old, 12-13 months); obesity.
P8783 Guo,Z.H., Jiang,H.Y., Xu,X.R., Duan,W.Z., Mattson,M.P. Leptin-mediated cell survival signaling in hippocampal neurons mediated by JAK STAT3 and mitochondrial stabilization. Journal of Biological Chemistry 2008; 283(-3-):1754-1763. >>> Leptin; PBS; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 3 used; peptides; animal info (male, C57BL/6, 3 months old); neurodegenerative.
P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).
P8753 Koch,L., Wunderlich,F.T., Seibler,J., Konner,A.C., Hampel,B., Irlenbusch,S., Brabant,G., Kahn,C.R., Schwenk,F., Bruening,J.C. Central insulin action regulates peripheral glucose and fat metabolism in mice. Journal of Clinical Investigation 2008; 118(-6-):2132-2147. >>> Leptin; insulin; SC; CSF/CNS; Mice; 1002; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; ALZET brain infusion kit 3 used; peptides; cyanoacrylate adhesive; animal info (C57BL/6, wt, IR-d-wb, 10-14 wks old); cannula position confirmed post mortem with methylene blue.
P8700 Hindlet,P., Bado,A., Farinotti,R., Buyse,M. Long-term effect of leptin on H+ -coupled peptide cotransporter 1 activity and expression in vivo: Evidence in leptin-deficient mice. Journal of Pharmacology and Experimental Therapeutics 2007; 323(-1-):192-201. >>> Leptin, recomb.; PBS; SC; Rat; mice; 1007D; 2ML1; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin levels; peptides; animal info (male, Wistar, 250-275g; male, C57BL/6J, wt, ob/ob, 7 wks old).
P8686 Harris,R.B.S., Bartness,T.J., Grill,H.J. Leptin responsiveness in chronically decerebrate rats. Endocrinology 2007; 148(-10-):4623-4633. >>> Leptin; PBS; IP; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by serum levels; dose-response (fig. 3A); peptides; animal info (Sprague-Dawley, male, 275-300g, chronically decerebrate); endocrinology.
P8678 Gallardo,N., Bonzon-Kulichenko,E., Fernandez-Agullo,T., Molto,E., Gomez-Alonso,S., Blanco,P., Carrascosa,J.M., Ros,M., Andres,A. Tissue-specific effects of central leptin on the expression of genes involved in lipid metabolism in liver and white adipose tissue. Endocrinology 2007; 148(-12-):5604-5610. >>> Leptin; PBS; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; peptides; animal info (3 months old, Wistar, male); endocrinology.
P8625 Dixit,V.D., Yang,H.W., Sun,Y.X., Weeraratna,A.T., Youm,Y.H., Smith,R.G., Taub,D.D. Ghrelin promotes thymopoiesis during aging. Journal of Clinical Investigation 2007; 117(-10-):2778-2790. >>> Ghrelin; leptin, recomb. mouse; PBS; SC; Mice; 2 weeks; Controls received mp w/ vehicle; peptides; animal info (Balb/c; 2,4,14,20 and 24 months old, femail, C57BL/6, 2,68,18 months old); endocrinology.
P8599 Stocker,C.J., Wargent,E., O'Dowd,J., Cornick,C., Speakman,J.R., Arch,J.R.S., Cawthorne,M.A. Prevention of diet-induced obesity and impaired glucose tolerance in rats following administration of leptin to their mothers. American Journal of Physiology- Regulatory, Integrative and Comparative Physiology 2007; 292(-5-):R1810-R1818. >>> Leptin, rat; Saline, physiological; SC; Rat (pregnant); 28 days; Controls received mp w/ vehicle; functionality of mp verified by plasma leptin concentration; teratology; peptides; animal info (female, Wistar, 200-225 grams, gestational day 14).
P8588 Tannenbaum,G.S., Gurd,W., Lapointe,M. Leptin Is a Potent Stimulator of Spontaneous Pulsatile Growth Hormone (GH) Secretion and the GH Response to GH-Releasing Hormone. Endocrinology 1998; 139(-9-):3871-3875. >>> Leptin, recomb. mouse; PBS; CSF/CNS; Rat; 2001; 6-7 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; peptides; animal info (male, Sprague-Dawley, 225-300 grams); cannula placement verified with methylene blue dye.
P8586 Scarpace,P.J., Matheny,M., Zhang,Y., Cheng,K.Y., Tumer,N. Leptin antagonist reveals an uncoupling between leptin receptor signal transducer and activator of transcription 3 signaling and metabolic responses with central leptin resistance. J Pharmacol.Exp.Ther. 2007/2; 320(-2-):706-712. >>> Leptin antagonist; NaHCO3; CSF/CNS; 14 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; peptides; animal info (male, F344xBN, 3 months old, 275-375 grams).
P8580 Harris,R.B., Mitchell,T.D., Kelso,E.W., Flatt,W.P. Changes in environmental temperature influence leptin responsiveness in low- and high-fat-fed mice. Am J Physiol Regul Integr Comp Physiol 2007/7; 293(-1-):R106-R115. >>> Leptin, recomb. mouse; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; peptides; animal info (male, C57BL/6, 15 weeks old, 25-20 grams).
P8572 Surwit,R.S., Edwards,C.L., Murthy,S., Petro,A.E. Transient effects of long-term leptin supplementation in the prevention of diet-induced obesity in mice. Diabetes 2000; 49(-7-):1203-1208. >>> Leptin, recomb. mouse; SC; Mice; 2004; 12 weeks; Controls received mp w/ PBS; functionality of mp verified by plasma levels and residual volume; long-term study; pumps replaced every 4 weeks; no stress (see pg. 1205); peptides; animal info (male, C57BL/6J, B6, A/J, 20 grams); obesity.
P8571 Ioffe,E., Moon,B., Connolly,E., Friedman,J.M. Abnormal regulation of the leptin gene in the pathogenesis of obesity. Proc Natl Acad Sci US A 1998; 95(-20-):11852-11857. >>> Leptin, recomb. murine; SC; Mice (transgenic); 28 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; animal info (male, C57BL/6J ob/ob Tg).
P8560 Plum,L., Rother,E., Muenzberg,H., Wunderlich,F.T., Morgan,D.A., Hampel,B., Shanabrough,M., Janoschek,R., Koenner,A.C., Alber,J., Suzuki,A., Krone,W., Horvath,T.L., Rahmouni,K., Bruening,J.C. Enhanced leptin-stimulated Pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metabolism 2007; 6(-6-):431-445. >>> Leptin; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Peptides; animal info (male).
P8495 Turner,S.M., Roy,S., Sul,H.S., Neese,R.A., Murphy,E.J., Samandi,W., Roohk,D.J., Hellerstein,M.K. Dissociation between adipose tissue fluxes and lipogenic gene expression in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-4-):E1101-E1109. >>> Leptin, recomb. murine; SC; Mice; 28 days; Leptin plasma levels taken; peptides; obesity; animal info (6-7 weeks old, female, C57BL/6J); endocrinology.
P8490 Scherbarth,F., Rozman,J., Klingenspor,M., Brabant,G., Steinlechner,S. Wheel running affects seasonal acclimatization of physiological and morphological traits in the Djungarian hamster (Phodopus sungorus). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 293(-3-):R1368-R1375. >>> Leptin, recomb. mouse; HCL; SC; Hamster; 1007D; 14 days; Pumps replaced every seven days; peptides; animal info (5-7 month old, M&F).
P8473 Harris,R.B.S., Mitchell,T.D., Hebert,S. Leptin-Induced Changes in Body Composition in High Fat-Fed Mice1. EXPERIMENTAL BIOLOGY AND MEDICINE 2003; 228(-1-):24-32. >>> Leptin, recomb. mouse; IP; Mice; 1002; 13 days; Controls received mp w/ PBS; functionality of mp verified by serum leptin concentrations; comparison of IP injections vs. mp; peptides; animal info (female, C57BL/6J, 8 weeks old); obesity.
P8471 Fewlass,D.C., Noboa,K., Pi-Sunyer,F.X., Johnston,J.M., Yan,S.D., Tezapsidis,N. Obesity-related leptin regulates Alzheimer's A beta. FASEB J. 2004/12; 18(-15-):1870-1878. >>> Leptin, recomb. mouse; PBS; SC; Mice (transgenic); 2004; 4,8; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks; peptides; post op. care (lidocaine); neurodegenerative(Alzheimer's disease); animal info (male, female, Tg2576 APPswe, C57BL/6 wt, 10 wks old, 32 wks old); obesity.
P8468 Abe,Y., Ono,K., Kawamura,T., Wada,H., Kita,T., Shimatsu,A., Hasegawa,K. Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation. Am.J.Physiol Heart Circ.Physiol 2007/5; 292(-5-):H2387-H2396. >>> Leptin, recomb. mouse; SC; Mice; 2ML4; 4 weeks; Controls received mp w/ sterile PBS; no stress (see pg H2395); cardiovascular; peptides; animal info (C57/BL6, 12 wks old, MI coronary artery ligation); ischemia (cardiac).
P8390 Johnston,R.A., Theman,T.A., Terry,R.D., Williams,E.S., Shore,S.A. Pulmonary responses to acute ozone exposure in fasted mice: effect of leptin administration. Journal of Applied Physiology 2007; 102(-1-):149-156. >>> Leptin, recomb. mouse; PBS; HCR; NaOH; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentration; peptides; post op. care (buprenorphine hydrochloride); animal info (male, C57BL/6J, 8 wks old).
P8351 Verlaeten,O., Casery,C., Cavagna,S., Navilie,D., Giraudon,P., Belin,M.F., Begeot,M., Bernard,A. Identification of Urop11, a novel leptin-modulated gene that is upregulated in the hypothalamus of mice with virus-induced obesity. Journal of Molecular Endocrinology 2007; 38(-1-2-):3-17. >>> Leptin; PBS; SC; Mice; 1 week; Peptides; animal info (male, C57BL/6J, control or HFD obese, CDV infected).
P8324 Shimomura,I., Hammer,R.E., Ikemoto,S., Brown,M.S., Goldstein,J.L. Leptin reverses insulin resistance and diabetes mellitus in mice with congenital lipodystrophy. Nature 1999/9/2; 401(-6748-):73-76. >>> Leptin, recomb. mouse; PBS, Dulbecco's; SC; Mice (transgenic); 1002; 12 days; Controls received mp w/ vehicle; peptides; animal info (C57BL/6J, ob/ob, male, female, 14-16 weeks old).
P8262 Nogueiras,R., Tovar,S., Mitchell,S.E., Barrett,P., Rayner,D.V., Dieguez,C., Williams,L.M. Negative energy balance and leptin regulate neuromedin-U expression in the rat pars tuberalis. Journal of Endocrinology 2006; 190(-2-):545-553. >>> Leptin, recomb. human; Saline; CSF/CNS; Rat; 2ML2; 7 days; 24, 48 hours; Controls received mp w/ vehicle; dose-response (fig. 6); peptides; animal info (male, Sprague-Dawley, 8-10 wks old, 300g.); cannula placement confirmed by methylene blue staining; endocrinology.
P8222 Gavrilova,O., Marcus-Samuels,B., Leon,L.R., Vinson,C., Reitman,M.L. Leptin and diabetes in lipoatrophic mice. Nature 2000; 403(-6772-):850-. >>> Leptin; Mice (transgenic); 4 weeks; 6 days; Controls received mp w/ saline; dose-response (fig. 1); peptides; animal info (male, 7, 13 weeks old); mouse model of lipodystrophy.
P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).
P8206 Luque,R.M., Huang,Z.H., Shah,B., Mazzone,T., Kineman,R.D. Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-3-):E891-E899. >>> Leptin, recomb.mouse; Saline; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; peptides; animal info (male, C57BL/6J ob/ob or wt, 10 wks old, 45g.); endocrinology.
P8186 la-Fera,M.A., Choi,Y.H., Hartzell,D.L., Duan,J.H., Hamrick,M., Baile,C.A. Sensitivity of ob/ob mice to leptin-induced adipose tissue apoptosis. Obesity Research 2005; 13(-9-):1540-1547. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response (fig 1); peptides; animal info (female, ob/ob or wt, 15 wk old, 60-65 grams, 20-25 grams).
P8155 Burcelin,R., Kamohara,S. Acute stimulation of glucose metabolism in mice by leptin treatment. Nature 1997/1/1; 389(--):374-377. >>> Leptin; CSF, artificial; CSF/CNS (third ventricle); Mice; 10 days; Controls received mp w/ PBS; comparison of acute IV infusion vs. mp; CSF pumps replaced with leptin mp after 7 days; peptides; animal info (C57BL/6J +/+ lean, 25 grams); tissue perfusion (ventricle).
P8141 Huang,Q.L., Timofeeva,E., Richard,D. Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. Journal of Endocrinology 2006; 191(-1-):179-188. >>> Leptin, recomb. murine; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ PBS; peptides; animal info (male, Wistar, 270-290g.); tissue perfusion (ventricle).
P8088 Fulton,S., Pissios,P., Manchon,R.P., Stiles,L., Frank,L., Pothos,E.N., Maratos-Flier,E., Flier,J.S. Leptin regulation of the mesoaccumbens dopamine pathway. Neuron 2006; 51(-6-):811-822. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1003D; 1002; 3, 11, 14 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); peptides; animal info (male, C57BL/6J wt, ob/ob or Lepr (db/db); 4-10 wks old); mp primed 4-6 hrs. at 37 celcius.
P8040 Zhang,J., Matheny,M.K., Tuemer,N., Mitchell,M.K., Scarpace,P.J. Leptin antagonist reveals that the normalization of caloric intake and the thermic effect of food after high-fat feeding are leptin dependent. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2007; 292(-2-):R868-R874. >>> Leptin; leptin antagonist; Sodium bicarbonate; CSF/CNS; 2001; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; peptides; animal info (male, Fischer, Brown Norway); dental cement used to secure cannula.
P8014 izawa-Abe,M., Ogawa,Y., Masuzaki,H., Ebihara,K., Satoh,N., Iwai,H., Matsuoka,N., Hayashi,T., Hosoda,K., Inoue,G., Yoshimasa,Y., Nakao,K. Pathophysiological role of leptin in obesity-related hypertension. J.Clin.Invest 2000/5; 105(-9-):1243-1252. >>> Leptin, recomb. mouse; SC; Mice; 1003D; 2002; 3 days; Controls received mp w/ vehicle; blood pressure taken; leptin plasma levels; cardiovascular; peptides; animal info (10 weeks old, male, transgenic, leptin defficient, C57BL/6J).
P7907 Wagoner,B., Hausman,D.B., Harris,R.B.S. Direct and indirect effects of leptin on preadipocyte proliferation and differentiation. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 290(-6-):R1557-R1564. >>> Leptin, recomb rat; PBS; IP; Rat; 1007D; 5 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin concentrations; no stress (see pg. R1559); peptides; animal info (male, Sprague-Dawley, 320-340 g).
P7895 Chen,K., Li,F.H., Li,J., Cai,H.B., Strom,S., Bisello,A., Kelley,D.E., Friedman-Einat,M., Skibinski,G.A., McCrory,M.A., Szalai,A.J., Zhao,A.Z. Induction of leptin resistance through direct interaction of C-reactive protein with leptin. Nature Medicine 2006; 12(-4-):425-432. >>> Leptin, recomb. human; C-reactive protein, human; Saline; SC; Mice; mice (transgenic); 6, 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum concentrations; dose-response (fig.5); peptides; MRI; multiple pumps per animal (2); animal info (wt or ob/ob, 8 wk old, human CRPtg.).
P7888 da Silva,A.A., Tallam,L.S., Liu,J.K., Hall,J.E. Chronic antidiabetic and cardiovascular actions of leptin: role of CNS and increased adrenergic activity. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2006; 291(-5-):R1275-R1282. >>> Leptin; Saline; CSF/CNS; Rat; 2002; 10 days; Controls received IV saline; functionality of mp verified by plasma leptin concentrations; comparison of IV infusion vs. mp; cardiovascular; peptides; animal info (male, Sprague-Dawley, 275-325g.).
P7811 Krol,E., Duncan,J.S., Redman,P., Morgan,P.J., Mercer,J.G., Speakman,J.R. Photoperiod regulates leptin sensitivity in field voles, Microtus agrestis. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 2006; 176(-2-):153-163. >>> Leptin, recomb. mouse; PBS; SC; Vole; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by serum leptin levels; peptides; animal info (male, field vole (microtus agrestis), 20-23 wk old, 26g.); "the osmotic pumps were effective in delivering biologically active hormone to the animals." (pg. 160).
8. Melanin-concentrating Hormone
P8300 Messina,M.M., Overton,J.M. Cardiovascular effects of melanin-concentrating hormone. REGULATORY PEPTIDES 2007; 139(-1-3-):23-30. >>> Melanin-concentrating hormone; PBS; BSA; CSF/CNS; Rat; 1002; 10 days; Controls received mp w/ vehicle; dose-response (fig. 1); comparison of acute icv infusion vs. mp; peptides; post op. care (Bupivacaine); animal info (male, Long-Evans, 330g.).
P7245 Mashiko,S., Ishihara,A., Gomori,A., Moriya,R., Ito,M., Iwaasa,H., Matsuda,M., Feng,Y., Shen,Z., Marsh,D.J., Bednarek,M.A., MacNeil,D.J., Kanatani,A. Antiobesity effect of a melanin-concentrating hormone 1 receptor antagonist in diet-induced obese mice. Endocrinology 2005; 146(-7-):3080-3086. >>> Melanin-concentrating hormone 1 receptor antagonist; Propylene glycol; CSF, artificial; CSF/CNS; CSF/CNS (third ventricle); Mice; 1007D; 2002; 2004; 3, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 1 or 4 weeks of vehicle; ALZET brain infusion kit used; peptides; cyanoacrylate adhesive; post op. care (cefamedin); DEXA; cannula placement confirmed by Evans blue dye injection; animal info (C57BL/6); pumps used in lateral ventricle for one group of animals, and third ventricle for others. Third ventricle group used Plastics One cannulae.
P6233 Shearman,L.P., Camacho,R.E., Stribling,D.S., Zhou,D., Bednarek,M.A., Hreniuk,D.L., Feighner,S.D., Tan,C.P., Howard,A.D., Van der Ploeg,L.H.T., MacIntyre,D.E., Hickey,G.J., Strack,A.M. Chronic MCH-1 receptor modulation alters appetite, body weight and adiposity in rats. European Journal of Pharmacology 2003; 475(-1-3-):37-47. >>> Neuropeptide Y, rat; melanin-concentrating hormone-1, receptor agonist; melanin-concentrating hormone-1, receptor antagonist; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; dose-response (fig. 5); peptides; post op. care (antisedan); pump model incorrectly given as 2001 (p. 39); based on flow rate (.5 ul/hr) and duration (14 days) is actually model 2002 (p. 39).
P5605 Gomori,A., Ishihara,A., Ito,M., Mashiko,S., Matsushita,H., Yumoto,M., Ito,M., Tanaka,T., Tokita,S., Moriya,M., Iwaasa,H., Kanatani,A. Chronic intracerebroventricular infusion of MCH causes obesity in mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2003; 284(-3-):E583-E588. >>> Melanin-concentrating hormone; Propylene glycol; CSF/CNS; Mice; 2001; 14 days; Controls received mp/ w vehicle; peptides; ALZET brain infusion kit used; 1st pump with vehicle only to allow for recovery (7-14 days), then the pump was replaced for MCH infusion; obesity.
P5509 Della-Zuana,O., Presse,F., Ortola,C., Duhault,J., Nahon,J.L., Levens,N. Acute and chronic administration of melanin-concentrating hormone enhances food intake and body weight in Wistar and Sprague-Dawley rats. International Journal of Obesity 2002; 26(-10-):1289-1295. >>> Melanin-concentrating hormone; CSF, artificial; CSF/CNS; Rat; 2002; 12 days; Controls received mp w/ vehicle; peptides; ALZET brain infusion kit used; cannula placement verified following injection of Evans blue dye.
P5066 Marsh,D.J., Weingarth,D.T., Novi,D.E., Chen,H.Y., Trumbauer,M.E. Melanin-concentrating hormone 1 receptor-deficient mice are lean, hyperactive, and hyperphagic and have altered metabolism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES O F AMERICA 2002; 99(-5-):3240-3245. >>> Melanin-concentrating hormone; CSF, artificial; CSF/CNS (third ventricle); mice; 6 days; controls received mp w/ vehicle; comparison of ICV injections vs mp; peptides; cannula patency verified via cresyl violet staining; mice received vehicle pumps for 6 days; pump model not indicated; pumps used had release rate of 0.5 ul/hr..
9. Melanotan
P8659 Strader,A.D., Shi,H., Ogawa,R., Seeley,R.J., Reizes,O. The effects of the melanocortin agonist (MT-II) on subcutaneous and visceral adipose tissue in rodents. Journal of Pharmacology and Experimental Therapeutics 2007; 322(-3-):1153-1161. >>> Melanotan-II; PBS; SC; Rat; mice; 14 days; Controls received mp w/ vehicle; MRI; animal info (male, Long Evans, 225-250g; male, C57BL/6J, 5 wks old); Melanocortin receptor agonist.
P7055 Li,G., Zhang,Y., Wilsey,J.T., Scarpace,P.J. Unabated anorexic and enhanced thermogenic responses to melanotan II in diet-induced obese rats despite reduced melanocortin 3 and 4 receptor expression. Journal of Endocrinology 2004; 182(-1-):123-132. >>> Leptin, recomb. mouse; melanotan II; CSF, artificial; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used.
P6877 Zhang,Y., Matheny,M., Tuemer,N., Scarpace,P.J. Aged-obese rats exhibit robust responses to a melanocortin agonist and antagonist despite leptin resistance. NEUROBIOLOGY OF AGING 2004; 25(-10-):1349-1360. >>> Melanotan II; SHU-9119; CSF, artificial; CSF/CNS; Rat; 14,21 days; pumps replaced after 7 days; all animals received an initial 7 day aCSF infusion.
P6874 Banno,R., Arima,H., Sato,I., Hayashi,M., Goto,M., Sugimura,Y., Murase,T., Oiso,Y. The melanocortin agonist melanotan II increases insulin sensitivity in OLETF rats. Peptides 2004; 25(-8-):1279-1286. >>> Melanotan II; Saline; SC; Rat; 2ML4; 25 days; Controls received mp w/ vehicle.
P6066 Scarpace,P.J., Matheny,M., Zolotukhin,S., Tumer,N., Zhang,Y. Leptin-induced leptin resistant rats exhibit enhanced responses to the melanocortin agonist MT II. Neuropharmacology 2003; 45(-2-):211-219. >>> Leptin; melanotan II; CSF, artificial; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; obesity; melanotan II (MT II) is a melanocortin receptor agonist; leptin was infused for 7 days, then the pump was replaced with MTII for another 7 days; pump model was not listed.
P5852 Raposinho,P.D., White,R.B., Aubert,M.L. The melanocortin agonist Melanotan-II reduces the orexigenic and adipogenic effects of neuropeptide Y (NPY) but does not affect the NPY-driven suppressive effects on the gonadotropic and somatotropic axes in the male rat. Journal of Neuroendocrinology 2003; 15(-2-):173-181. >>> Melanotan-II; Neuropeptide Y; PBS; Ascorbic Acid; BSA; CSF/CNS; Rat; 7 days; Peptides; Melanotan II or MTII (a melanocortin receptor agonist)&neuropeptide were dissolved in PBS, 0.01% ascorbic acid, 0.1% bovine serum albumin adjusted to ph 7.4; pump model not listed.
P5252 Lindblom,J., Kask,A., Hagg,E., Harmark,L., Bergstrom,L., Wikberg,J. Chronic infusion of a melanocortin receptor agonist modulates dopamine receptor binding in the rat brain. Pharmacol Res 2002/2; 45(-2-):119-124. >>> Melanotan-II; CSF, artificial; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; good methods (p.120); ALZET brain infusion kit used; Melanotan-II is a melanocortin receptor agonist.
10. Melanotan II
P7055 Li,G., Zhang,Y., Wilsey,J.T., Scarpace,P.J. Unabated anorexic and enhanced thermogenic responses to melanotan II in diet-induced obese rats despite reduced melanocortin 3 and 4 receptor expression. Journal of Endocrinology 2004; 182(-1-):123-132. >>> Leptin, recomb. mouse; melanotan II; CSF, artificial; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used.
P6877 Zhang,Y., Matheny,M., Tuemer,N., Scarpace,P.J. Aged-obese rats exhibit robust responses to a melanocortin agonist and antagonist despite leptin resistance. NEUROBIOLOGY OF AGING 2004; 25(-10-):1349-1360. >>> Melanotan II; SHU-9119; CSF, artificial; CSF/CNS; Rat; 14,21 days; pumps replaced after 7 days; all animals received an initial 7 day aCSF infusion.
P6874 Banno,R., Arima,H., Sato,I., Hayashi,M., Goto,M., Sugimura,Y., Murase,T., Oiso,Y. The melanocortin agonist melanotan II increases insulin sensitivity in OLETF rats. Peptides 2004; 25(-8-):1279-1286. >>> Melanotan II; Saline; SC; Rat; 2ML4; 25 days; Controls received mp w/ vehicle.
P6066 Scarpace,P.J., Matheny,M., Zolotukhin,S., Tumer,N., Zhang,Y. Leptin-induced leptin resistant rats exhibit enhanced responses to the melanocortin agonist MT II. Neuropharmacology 2003; 45(-2-):211-219. >>> Leptin; melanotan II; CSF, artificial; CSF/CNS; Rat; 7 days; Controls received mp w/ vehicle; leptin plasma levels taken; ALZET brain infusion kit used; obesity; melanotan II (MT II) is a melanocortin receptor agonist; leptin was infused for 7 days, then the pump was replaced with MTII for another 7 days; pump model was not listed.
11. Neuropeptide Y
P8684 Fuezesi,T., Wittmann,G., Liposits,Z., Lechan,R.M., Fekete,C. Contribution of noradrenergic and adrenergic cell groups of the Brainstem and agouti-related protein-synthesizing neurons of the arcuate nucleus to neuropeptide-y innervation of corticotropin-releasing hormone neurons in hypothalamic Paraventricular nucleus of the rat. Endocrinology 2007; 148(-11-):5442-5450. >>> Neuropeptide Y; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; peptides; post op. care (bacitracin ointment over skull); animal info (Sprague-Dawley, male, 280-320g); 22-gauge stainless steel guide cannula, Plastics One.
P8515 Ishii,T., Muranaka,R., Tashiro,O., Nishimura,M. Chronic intracerebroventricular administration of anti-neuropeptide Y antibody stimulates starvation-induced feeding via compensatory responses in the hypothalamus. Brain Research 2007; 1144(--):91-100. >>> Antibody, rabbit, anti-neuropeptide Y; Saline; CSF/CNS; Mice; 2002; 13 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; ALZET brain infusion kit 3 used; animal info (male, ddy, 7 weeks old, 35-40 g).
P8089 Gebhard,C., Petroktistis,F., Zhang,H., Kammerer,D., Koehle,C., Klingel,K., Albinus,M., Gleiter,C.H., Osswald,H., Grenz,A. Role of renal nerves and salt intake on erythropoietin secretion in rats following carbon monoxide exposure. Journal of Pharmacology and Experimental Therapeutics 2006; 319(-1-):111-116. >>> Isoproterenol; neuropeptide Y, [Leu31, Pro34]-; neuropeptide Y1 receptor antagonist; IV (jugular); Rat; 1003D; 16 hours; Controls received mp w/ vehicle; half-life (p.115) "short" for isoproterenol; peptides; animal info (male, Sprague-Dawley, 250-320g.).
P7215 Michalkiewicz,M., Zhao,G.Q., Jia,Z., Michalkiewicz,T., Racadio,M.J. Central neuropeptide Y signaling ameliorates N omega-nitro-L-arginine methyl ester hypertension in the rat through a Y1 receptor mechanism. Hypertension 2005; 45(-4-):780-785. >>> Neuropeptide Y, synthetic rat; BIBP3226; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; cardiovascular; antihypertensive; peptides; cannula placement confirmed by methylene blue staining; "This approach (mp) allowed us to assess the role of endogenous NPY in long-term control of BP under experimental conditions that excluded complications associated with anesthesia, restraint, and short-term drug administration." (p. 782); neuropeptide Y, Y1 receptor antagonist.
P6952 Henry,M., Ghibaudi,L., Gao,J., Hwa,J.J. Energy metabolic profile of mice after chronic activation of central NPY Y1, Y2, or Y5 receptors. Obesity Research 2005; 13(-1-):36-47. >>> Neuropeptide Y; Y1 agonist; Y5 agonist; Saline; water; ascorbic acid; CSF/CNS; Mice; 2001; 6 days; Controls received mp w/ vehicle; peptides; mice received a two week recovery period; guide cannula used and secured with tissue adhesive; animal info (6 week old, C57BL/6, 25-30 grams).
P6495 Raposinho,P.D., Pedrazzini,T., White,R.B., Palmiter,R.D., Aubert,M.L. Chronic neuropeptide Y infusion into the lateral ventricle induces sustained feeding and obesity in mice lacking either Npy1r or Npy5r expression. Endocrinology 2004; 145(-1-):304-310. >>> Neuropeptide Y, porcine; Phosphate buffer; sodium chloride; ascorbic acid; BSA; CSF/CNS; Mice; 2001; 7 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg.305) initial weight loss from surgery/anesthesia; ALZET brain infusion kit 2 used; peptides; cyanoacrylate adhesive (Loctite 454).
P7706 Mashiko,S., Ishihara,A., Iwaasa,H., Sano,H., Oda,Z., Ito,J., Yumoto,M., Okawa,M., Suzuki,J., Fukuroda,T., Jitsuoka,M., Morin,N.R., MacNeil,D.J., Van der Ploeg,L.H., Ihara,M., Fukami,T., Kanatani,A. Characterization of neuropeptide Y (NPY) Y5 receptor-mediated obesity in mice: chronic intracerebroventricular infusion of D-Trp34NPY. Endocrinology 2003/5; 144(-5-):1793-1801. >>> Neuropeptide Y, D-Trp34; PBS; BSA; CSF/CNS; Mice; 2001; 2002; 13-21 days; Controls received mp w/ vehicle; dose-response (fig. 1); pumps replaced after 7-14 days; ALZET Brain Infusion kit used; peptides; post op. care (cefamedin); animal info (C57BL/6J, 9-12 weeks old, male); cannula placement confirmed by Evans blue dye injection.
P6233 Shearman,L.P., Camacho,R.E., Stribling,D.S., Zhou,D., Bednarek,M.A., Hreniuk,D.L., Feighner,S.D., Tan,C.P., Howard,A.D., Van der Ploeg,L.H.T., MacIntyre,D.E., Hickey,G.J., Strack,A.M. Chronic MCH-1 receptor modulation alters appetite, body weight and adiposity in rats. European Journal of Pharmacology 2003; 475(-1-3-):37-47. >>> Neuropeptide Y, rat; melanin-concentrating hormone-1, receptor agonist; melanin-concentrating hormone-1, receptor antagonist; Saline; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; dose-response (fig. 5); peptides; post op. care (antisedan); pump model incorrectly given as 2001 (p. 39); based on flow rate (.5 ul/hr) and duration (14 days) is actually model 2002 (p. 39).
P6110 Takahashi,K., Mizuarai,S., Araki,H., Mashiko,S., Ishihara,A., Kanatani,A., Itadani,H., Kotani,H. Adiposity elevates plasma MCP-1 levels leading to the increased CD11b-positive monocytes in mice. Journal of Biological Chemistry 2003; 278(-47-):46654-46660. >>> Neuropeptide Y; monocyte chemoattractant protein-1; PBS; BSA; CSF/CNS; Mice; 2002; 2 weeks; Controls received mp w/ vehicle; mcp-1 plasma levels taken; ALZET brain infusion kit used; peptides; obesity; 7-14 day recovery period after surgery; pumps filled with PBS during recovery; monocyte chemoattractant protein-1 is also known as MCP-1.
P5852 Raposinho,P.D., White,R.B., Aubert,M.L. The melanocortin agonist Melanotan-II reduces the orexigenic and adipogenic effects of neuropeptide Y (NPY) but does not affect the NPY-driven suppressive effects on the gonadotropic and somatotropic axes in the male rat. Journal of Neuroendocrinology 2003; 15(-2-):173-181. >>> Melanotan-II; Neuropeptide Y; PBS; Ascorbic Acid; BSA; CSF/CNS; Rat; 7 days; Peptides; Melanotan II or MTII (a melanocortin receptor agonist)&neuropeptide were dissolved in PBS, 0.01% ascorbic acid, 0.1% bovine serum albumin adjusted to ph 7.4; pump model not listed.
P6193 Fekete,C., Sarkar,S., Rand,W.M., Harney,J.W., Emerson,C.H., Bianco,A.C., Beck-Sickinger,A., Lechan,R.M. Neuropeptide Y1 and Y5 receptors mediate the effects of neuropeptide Y on the hypothalamic-pituitary-thyroid axis. Endocrinology 2002; 143(-12-):4513-4519. >>> Neuropeptide Y; neuropeptide Y, receptor agonist; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; peptides; endocrinology.
P5583 Fekete,C., Sarkar,S., Rand,W.M., Harney,J.W., Emerson,C.H., Bianco,A.C., Lechan,R.M. Agouti-related protein (AGRP) has a central inhibitory action on the hypothalamic-pituitary-thyroid (HPT) axis; Comparisons between the effect of AGRP and neuropeptide Y on energy homeostasis and the HPT axis. Endocrinology 2002; 143(-10-):3846-3853. >>> Neuropeptide Y; Agouti-related protein; CSF, artificial; CSF/CNS; rat; 1003D; 3 days; peptides; cannula was implanted then occluded with a dummy cannula for one week prior to infusion to allow for recovery; AGRP is an appetite stimulant.
P5254 Toufexis,D.J., Yorozu,S., Woodside,B. Y1 receptor activation is involved in the effect of exogenous neuropeptide Y on pup growth and the early termination of lactational diestrus in the postpartum rat. J Neuroendocrinol. 2002/5; 14(-5-):354-360. >>> Neuropeptide Y; 1229U91; Neuropeptide Y, receptor agonist; Ascorbic acid; Phosphate buffer; NaCl; BSA; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; cannua placement verified manually by locating the tip postmortem; 1229U91 is a mixed Y1 antagonist/Y4 agonist.
P5234 Correia,M.L., Morgan,D.A., Sivitz,W.I., Mark,A.L., Haynes,W.G. Hemodynamic consequences of neuropeptide Y-induced obesity. Am.J Hypertens 2002/2; 15(-2 Pt 1-):137-142. >>> Neuropeptide Y; Saline; CSF/CNS (third ventricle); Rat; 2004; 2 weeks; Controls received mp w/ vehicle; Peptides: cannula placement verified by methylene blue staining; one-week recovery period.
P5015 Kramer,A., Yang,F.C., Snodgrass,P., Li,X., Scammell,T.E., Davis,F.C., Weitz,C.J. Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling. Science 2001/12/21; 294(-5551-):2511-2515. >>> Transforming growth factor-a; Brain-derived neurotrophic factor; Vasoactive intestinal polypeptide; Peptide, histidine-isoleucine; Gastrin releasing peptide; Substance P; Neuromedin-C; Neurokinin A; Neuropeptide K; Neuropeptide Y; Somatostatin; Antrin; Cholecystokinin; Thyrotropin-releasing hormone; Neurotensin; Neuromedin N;; CSF, artificial; CSF/CNS (third ventricle); hamster; 2002; 18-22 days; peptides.
P4311 Pierroz,D.D., Aebi,A.C., Huhtaniemi,I.T., Aubert,M.L. Many LH peaks are needed to physiologically stimulate testosterone secretion: modulation by fasting and NPY. Am.J.Physiol.(Endocrinol.Metab.39) 1999; 276(--):E603-E610. >>> Neuropeptide Y;; Saline; Ascorbic acid; BSA;; CSF/CNS;; rat;; 2001;; 1 week;; controls received no treatment; peptides; vehicle was filter sterilized;.
P4132 McMinn,J.E., Seeley,R.J., Wilkinson,C.W., Havel,P.J., Woods,S.C., Schwartz,M.W. NPY-induced overfeeding suppresses hypothalamic NPY mRNA expression: potential roles of plasma insulin and leptin. Regul.Pept. 1998; 75-76(--):425-431. >>> Neuropeptide Y, human; CSF, artificial;; CSF/CNS (third ventricle); rat; 2002; no duration posted; controls received mp w/vehicle; cannula placement verified one week after placement by icv injection of angiotensin II; pumps implanted 3 weeks after cannula placement; peptides.
P4124 Al-Arabi,A., Andrews,J.F. Synergistic action by neuropeptide y (NPY) and norepinephrine (NE) on food intake, metabolic rate, and brown adipose tissue (bat) causes remarkable weight loss in the obese (fa/fa) Zucker rat. Biomed.Sci.Instrum. 1997; 33(--):216-225. >>> Neuropeptide Y; Norepinephrine; SC; rat; 2002; 14 days; controls received mp w/vehicle; agents infused alone or in combination; peptides.
P3830 White,D. Intrathecal neuropeptide Y exacerbates nerve injury-induced mechanical hyperalgesia. Brain Research 1997; 750(--):141-146. >>> Neuropeptide Y; Trinositol, a-; Saline; Heparin; CSF/CNS (intrathecal); rat; 14 days; no comment posted.
P4109 Pierroz,D.D., Catzeflis,C., Aebi,A.C., Rivier,J.E., Aubert,M.L. Chronic administration of neuropeptide Y into the lateral ventricle inhibits both the pituitary-testicular axis and growth hormone and insulin-like growth factor I secretion in intact adult male rats. Endocrinology 1996; 137(-1-):3-12. >>> Neuropeptide Y, synthetic porcine; Neuropeptide Y-(13-36); CSF/CNS; rat; 2001; 7 days; controls received sham surgery; dose-response (6, 18, 36 ug/d); peptides.
P3462 White,D.M., Mansfield,K. Vasoactive intestinal polypeptide and neuropeptide Y act indirectly to increase neurite outgrowth of dissociated dorsal root ganglion cells. Neuroscience 1996; 73(-3-):881-887. >>> Vasoactive intestinal peptide; Neuropeptide Y; VIP antagonist; Trinositol, a-; Heparin; Saline; CSF/CNS (spinal cord, intrathecal); rat; 2002; 2 weeks; Peptides; 10 U/ml of heparin.
P2710 Dube,M.G., Xu,B., Crowley,W.R., Kalra,P.S., Kalra,S.P. Evidence that neuropeptide Y is a physiological signal for normal food intake. Brain Research 1994; 646(--):341-344. >>> Antibody, neuropeptide Y; Immunoglobulin, anti-neuropeptide Y; Serum, normal rabbit; CSF, artificial; Saline; Immunoglobulin, normal rabbit serum; CSF/CNS (third ventricle); rat; 2001D; 24 hours; controls received mp w/ rabbit serum; peptides.
P2624 Zelis,R., Nussberger,J., Clemson,B., Waeber,B., Grouzmann,E., Brunner,H.R. Neuropeptide Y infusion decreases plasma renin activity in postmyocardial infarction rats. J.Cardiovasc.Pharmacol. 1994; 24(-6-):896-899. >>> Neuropeptide Y; Sodium chloride; IV (jugular); rat; 1 week; controls received mp w/ vehicle.
P3087 Xu,B., Sahu,A., Crowley,W.R., Leranth,C., Horvath,T., Kalra,S.P. Role of neuropeptide-Y in episodic luteinizing hormone release in ovariectomized rats: an excitatory component and opioid involvement. Endocrinology 1993; 133(-2-):747-754. >>> Antibody, neuropeptide Y; Serum, normal rabbit; CSF, artificial;; CSF/CNS (third ventricle); rat; 2001D; no duration posted; controls received mp with vehicle.
P3078 Zarjevski,N., Cusin,I., Vettor,R., Rohner-Jeanrenaud,F., Jeanrenaud,B. Chronic intracerebroventricular neuropeptide-Y administration to normal rats mimics hormonal and metabolic changes of obesity. Endocrinology 1993; 133(-4-):1753-1758. >>> Neuropeptide Y, porcine; PBS; Albumin, bovine serum; Ascorbic acid; CSF/CNS; rat; 2001; 7 days; controls received mp with vehicle; peptides; stylet maintained cannula patency for 1 week after placement.
P2731 Gruaz,N.M., Pierroz,D.D., Rohner-Jeanrenaud,F., Sizonenko,P.C., Aubert,M.L. Evidence that neuropeptide Y could represent a neuroendocrine inhibitor of sexual maturation in unfavorable metabolic conditions in the rat. Endocrinology 1993; 133(-4-):1891-1894. >>> Neuropeptide Y, synthetic porcine; Sodium chloride; Ascorbic acid; Albumin, bovine serum; PBS; CSF/CNS; rat; 2001; 7 days; controls were food-restricted and received mp w/ vehicle or were fed ad libitium; peptides.
P2158 Catzeflis,C., Pierroz,D.D., Rohner-Jeanrenaud,F., Rivier,J.E., Sizonenko,P.C., Aubert,M.L. Neuropeptide Y administered chronically into the lateral ventricle profoundly inhibits both the gonadotropic and the somatotropic axis in intact adult female rats. Endocrinology 1993; 132(-1-):224-234. >>> Neuropeptide Y, porcine; Ascorbic acid; Albumin, bovine serum; PBS; CSF/CNS; rat; 2001; 7 days; controls received mp with vehicle; dose-response (graph p. 227); peptides.
P1983 Beck,B., Stricker-Krongrad,A., Nicolas,J., Burlet,A. Chronic and continuous intracerebroventricular infusion of neuropeptide Y in Long-Evans rats mimics the feeding behaviour of obese Zucker rats. Int.J.Obes. 1992; 16(--):295-302. >>> Neuropeptide Y; CSF, artificial; CSF/CNS; rat; 2002; no duration posted; functionality of mp verified by measurement of residual volume; stress/adverse reaction: small weight loss in animals with vehicle-containing pumps p298; stability verified in vitro at 37 C for 14 days;peptides.
P1961 Beck,B., Stricker-Krongrad,A., Nicolas,J.P., Burlet,C. Chronic and continuous ICV infusion of neuropeptide Y disrupts the nycthemeral feeding patterns in rats. Ann.N.Y.Acad.Sci. 1990; 611(--):491-494. >>> Neuropeptide Y; CSF, artificial; rat; 2002; 2 weeks; peptides.
P1716 Waeber,B., Evequoz,D., Aubert,J.-F., Fluckiger,J.-P., Juillerat,L., Nussberger,J., Brunner,H.R. Prevention of renal hypertension in the rat by neuropeptide Y. J.Hypertens. 1990; 8(--):21-25. >>> Neuropeptide Y; Saline; IV (jugular); rat; 1702; 8-9 days; peptides.
12. Peptide YY
P9025 Unniappan,S., Kieffer,T.J. Leptin extends the anorectic effects of chronic PYY(3-36) administration in ad libitum-fed rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-1-):R51-R58. >>> Peptide YY (3-36), rat; peptide YY (3-36), human; leptin; Saline; SC; Rat; 2001D; 2ML1; 2ML2; 7 days; 24 hours; Controls received mp w/ vehicle; animal info (Fischer 344, 224 g.); 14-day pumps were removed after 7 days, and reimplanted into a new set of animals; "In the coadministration studies, a single pump was used to deliver both PYY (3-36) and leptin." pg. R53; "PYY (3-36) remained biologically active while in the pumps for at least 9 days.".
P8987 Batterham,R.L., Heffron,H., Kapoor,S., Chivers,J.E., Chandarana,K., Herzog,H., Le Roux,C.W., Thomas,E.L., Bell,J.D., Withers,D.J. Critical role for peptide YY in protein-mediated satiation and body-weight regulation. Cell Metabolism 2006; 4(-3-):223-233. >>> Peptide YY (3-36); PBS; SC; Mice; 2004; 3 weeks; Controls received mp w/ vehicle; replacement therapy (Pyy null); comparison of IP injections vs. mp; peptides; animal info (C57BL/6, 12 wks old, wt, Pyy -/-, male; 22 wks old, 30-40 g.).
P8688 Roth,J.D., Coffey,T., Jodka,C.M., Maier,H., Athanacio,J.R., Mack,C.M., Weyer,C., Parkes,D.G. Combination therapy with amylin and peptide YY[3-36] in obese rodents: Anorexigenic synergy and weight loss additivity. Endocrinology 2007; 148(-12-):6054-6061. >>> Amylin; peptide YY (3-36), human; DMSO; SC; Rat; mice; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; dose-response (fig. 2, p. 6057); peptides; animal info (rats, DIO prone; mice, DIO, C57BL/6J); 50% DMSO in water.
P8299 Cruze,C.A., Su,F., Limberg,B.J., Deutsch,A.J., Stoffolano,P.J., Dai,H.J., Buchanan,D.D., Yang,H.T., Terjung,R.L., Spruell,R.D., Mittelstadt,S.W., Rosenbaum,J.S. The Y2 receptor mediates increases in collateral-dependent blood flow in a model of peripheral arterial insufficiency. Peptides 2007; 28(-2-):269-280. >>> Peptide YY, recomb. human; peptide YY (3-36), recomb. human; vascular endothelial growth factor 165; PBS; glycerol; sodium citrate; Tween 20; IA (iliac); Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (femoral artery occlusion); dose-response (fig. 4); half-lfie (p. 276)<30 minutes; cardiovascular; peptides; ischemia (hindlimb); animal info (male, Sprague-Dawley, 325-350 grams).
P8292 van den Hoek,A.M., Heijboer,A.C., Voshol,P.J., Havekes,L.M., Romijn,J.A., Corssmit,E.P.M., Pijl,H. Chronic PYY3-36 treatment promotes fat oxidation and ameliorates insulin resistance in C57BL6 mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-1-):E238-E245. >>> Peptide YY (3-36); Saline; SC; Mice; 2001; 7 days; Controls received mp w/ vehicle; plasma levels taken; comparison of bolus injection vs. mp; animal info (C57BL/6, male, 3 months old, 35 grams); diabetes; gut hormone.
P8015 Vrang,N., Madsen,A.N., Tang-Christensen,M., Hansen,G., Larsen,P.J. PYY(3-36) reduces food intake and body weight and improves insulin sensitivity in rodent models of diet-induced obesity. Am.J.Physiol Regul.Integr.Comp Physiol 2006/8; 291(-2-):R367-R375. >>> Peptide YY (3-36); Saline, isotonic; SC; Mice; rat; 2004; 2ML4; 28 days; Stability verified by in vitro test, degradation was<0.5%/day; peptides; animal info (C57BL/6, DIO and rats, Wistar and Sprague-Dawley); gut hormone; SC administration of PYY (3-36) for 28 days reduced body weight and improved glycemic control.
P7952 Unniappan,S., McIntosh,C.H.S., Demuth,H.U., Heiser,U., Wolf,R., Kieffer,T.J. Effects of dipeptidyl peptidase IV on the satiety actions of peptide YY. Diabetologia 2006; 49(-8-):1915-1923. >>> Peptide YY (3-36), rat; peptide YY (1-36), human; peptide YY (1-36), rat; Saline; SC; Rat; 2001D; 2ML2; 6 days; 24 hours; Controls received mp w/ vehicle; no stress (see pg. 1919); animal info (Fischer 344, 200-225 grams).
P7943 Tung,Y.C.L., Hewson,A.K., Carter,R.N., Dickson,S.L. Central responsiveness to a ghrelin mimetic (GHRP-6) is rapidly altered by acute changes in nutritional status in rats. Journal of Neuroendocrinology 2005; 17(-6-):387-393. >>> Peptide YY (3-36), recomb. human; Saline; IP; Rat; 2001; 6 days; Controls received mp w/ vehicle; peptides; animal info (adult, male, Wistar, 200-250 grams).
P7687 Adams,S.H., Lei,C.L., Jodka,C.M., Nikoulina,S.E., Hoyt,J.A., Gedulin,B., Mack,C.M., Kendall,E.S. PYY[3-36] administration decreases the respiratory quotient and reduces adiposity in diet-induced obese mice. Journal of Nutrition 2006; 136(-1-):195-201. >>> Peptide YY (3-36); Water; DMSO; SC; Mice; 1003D; 2001; 2004; 3,7,28 days; Animal info (obese, male, C57BL/6J); obesity; gut derived hormone; 50% DMSO used.
P7540 Babu,M., Purhonen,A.K., Bansiewicz,T., Maekelae,K., Walkowiak,J., Miettinen,P., Herzig,K.H. Effect of total colectomy and PYY infusion on food intake and body weight in rats. REGULATORY PEPTIDES 2005; 131(-1-3-):29-33. >>> Peptide YY (3-36); PBS; IV (jugular); Rat; 2ML2; 14 days; Controls received mp w/ vehicle; replacement therapy (proctocolectomy); no stress (see pg. 31-32) "no signs of stress... were found at any time during our studies"; peptides; post op. care (buprenorphine); animal info (male, Wistar 300-395 g); "we chose continuous infusion from subcutaneous implanted mini pumps to mimic physiological conditions and minimize stress caused by interperitoneal injections of the peptide." (p. 32).
P7429 Grise,K.R., McFadden,D.W. Peptide YY improves local and systemic parameters and prevents death in lethal necrotizing pancreatitis. Pancreas 2002/1; 24(-1-):90-95. >>> Peptide YY; SC; Mice; 3,4,6 days; 24,48 hours; Controls received mp w/ normal saline; peptides.
P6794 Pittner,R.A., Moore,C.X., Bhavsar,S.P., Gedulin,B.R., Smith,P.A., Jodka,C.M., Parkes,D.G., Paterniti,J.R., Srivastava,V.P., Young,A.A. Effects of PYY[3-36] in rodent models of diabetes and obesity. International Journal of Obesity 2004; 28(-8-):963-971. >>> Peptide YY (3-36); PBS; SC; Rat; mice; 2004; 2ML4; 4, 8 weeks; Controls received mp w/ vehicle; dose-response (fig. 1); comparison of IP injections vs. mp; long-term study; pumps replaced after 4 weeks; diabetes; obesity; peptide.
P4082 Liu,C.D., Slice,L.W., Balasubramaniam,A., Walsh,J.H., Newton,T.R., Saxton,R.E., McFadden,D.W. Y2 receptors decrease human pancreatic cancer growth and intracellular cyclic adenosine monophosphate levels. Surgery 1995; 118(--):229-236. >>> Peptide YY; BIM-43004-1; mice (nude); 2,3,4 weeks; controls received mp w/saline; cancer; peptide YY is an enteric hormone; BIM-43004-1 is a modified PYY (22-36) Y2 receptor agonist; peptides.
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“We switched to a 21-day minipumps paradigm using a dose that mimics serum antidepressant concentrations in humans because this regimen resulted in a more consistent and robust decrease in serotonin transporter levels in rats than what is observed with daily injections of serotonin reuptake inhibitors.” Weinshenker et al. 2002 Brain Research 946:239-246.