References on the Use of ALZET® Osmotic Pumps Where Post Operative Care Was Mentioned

16353 Gong, K., Bhargava, A., Jasmin, L. GluN2B N-methyl-D-aspartate receptor and excitatory amino acid transporter 3 are upregulated in primary sensory neurons after 7 days of morphine administration in rats: implication for opiate-induced hyperalgesia. Pain 2016; 157(-1-):147-58. >>> Morphine; Ro 25-6981; Saline; SC; Rat; 2ML1; Controls received mp w/ vehicle; animal info (Male Sprague-dawley rats, 220-250 g); functionality of mp verified by behavioral tests; good methods (pg. 148); post op. care (opiate administration using pumps); behavioral testing (Hargreaves plantar test); Dependence/tolerance induced; Opiate use; Dose ( 15 mg/ml Morphine, 5 mg/kg Ro 25-6981);.

16337 Figge, D. A., Eskow Jaunarajs, K. L., Standaert, D. G. Dynamic DNA Methylation Regulates Levodopa-Induced Dyskinesia. J Neurosci 2016; 36(-24-):6514-24. >>> RG108; Cyclodextrin, hydroxypropyl-B-; CSF/CNS (dorsal striatum); Rat; 2004; Controls received mp w/ vehicle; animal info (male Sprague-dawley, 60– 90 days old, 180-200 g); functionality of mp verified by behavioral testing; 5% cyclodextrin used; Plastics One unilateral guide cannula used; post op. care (7 days of care, buprenorphine and wound care for pain management); behavioral testing (forepaw adjusting steps test); tissue perfusion (brain); delayed delivery “additional polyethylene tubing was added to provide a 14 d prime of vehicle before RG-108 administration” (pg. 6515); “rats were given a unilateral dopamine lesion to the left medial forebrain bundle” (pg. 6515); Brain coordinates: anteroposterior, 0 from bregma, -3mmlateral from midline, and -3.5 mm from the dura; Dose (100 uM);.

16331 Dudek, M., Hyytia, P. Alcohol preference and consumption are controlled by the caudal linear nucleus in alcohol-preferring rats. Eur J Neurosci 2016; 43(-11-):1440-8. >>> Manganese Chloride; Saline, Tris-buffered; SC; Rat; 2001; animal info (male alcohol-preferring AA and male Wistar rats; 6 weeks old, 264-413 g); functionality of mp verified by MRI after pump removal; post op. care (carprofen injection); Dose (120 mg/kg/wk);.

16315 Carusillo Theriault, B., Woo, S. K., Karimy, J. K., Keledjian, K., Stokum, J. A., Sarkar, A., Coksaygan, T., Ivanova, S., Gerzanich, V., Simard, J. M. Cerebral microbleeds in a neonatal rat model. PLoS One 2017; 12(-2-):e0171163. >>> Endotoxin, LPS; IV (jugular); Rat (Pregnant); 2001D; animal info (Pregnant Wistar); post op. care (Buprenorphine (0.3±0.5 mg/kg) every 12 hours, as needed.); Dose (600 ng/hr); Resultant plasma level (4.5+/-0.9 EU/mL LPS);.

16285 Di Siena, S., Gimmelli, R., Nori, S. L., Barbagallo, F., Campolo, F., Dolci, S., Rossi, P., Venneri, M. A., Giannetta, E., Gianfrilli, D., Feigenbaum, L., Lenzi, A., Naro, F., Cianflone, E., Mancuso, T., Torella, D., Isidori, A. M., Pellegrini, M. Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury. Cell Death Dis 2016; 7(-7-):e2317. >>> Uridine, Bromodeoxy; SC; mice; post op. care (500Ǩl glucose solution (5% glucose/physiologic solution), analgesic Atradol (3 mg/kg); Dose (0.6 M);.

16273 Wilkinson, R., Song, W., Smoktunowicz, N., Marston, S. A dilated cardiomyopathy mutation blunts adrenergic response and induces contractile dysfunction under chronic angiotensin II stress. Am J Physiol Heart Circ Physiol 2015; 309(-11-):H1936-46. >>> Angiotensin II; Saline; SC; mice; 1004; Controls received mp w/ vehicle; animal info (mice (8 wk old)); functionality of mp verified by echocardiography; dose-response (p. H1938, H1941); good methods (p. H1938); post op. care (buprenorphine); cardiovascular; 7 mm Reflex used; Dose (1.4 and 2.0 mg/kg/day);.

16267 Varvel, N. H., Grathwohl, S. A., Degenhardt, K., Resch, C., Bosch, A., Jucker, M., Neher, J. J. Replacement of brain-resident myeloid cells does not alter cerebral amyloid-beta deposition in mouse models of Alzheimer's disease. J Exp Med 2015; 212(-11-):1803-9. >>> Valganciclovir; PBS; CSF/CNS (ventricle); mice; 2004; Controls received mp w/ vehicle; animal info (Female hemizygous TK mice crossed w/ male hemizygous APPPS1 or hemizygous APP23); ALZET brain infusion kit 3 used; good methods (pg. 11807); post op. care (paracetamol, i.p. 5 mg/kg daily; acetaminophen i.p. 5 mg/kg daily); brain tissue distribution; Dental cement used; Cannula placement verified via staining; anesthetized using ketamine and xylazine; Brain coordinates; from bregma: +0.1 mm anteroposterior, 1.0 mm lateral, and 2.5 mm dorsoventral; Dose (50 mg/ml);.

16250 Smith, P. Y., Hernandez-Rapp, J., Jolivette, F., Lecours, C., Bisht, K., Goupil, C., Dorval, V., Parsi, S., Morin, F., Planel, E., Bennett, D. A., Fernandez-Gomez, F. J., Sergeant, N., Buee, L., Tremblay, M. E., Calon, F., Hebert, S. S. miR-132/212 deficiency impairs tau metabolism and promotes pathological aggregation in vivo. Hum Mol Genet 2015; 24(-23-):6721-35. >>> miR-132 mimics; In vivo transfection agent; CSF/CNS (ventricle); mice; 2006; Controls received mp w/ vehicle; animal info (Twelve-month-old 3xTg-AD WT mice); ALZET brain infusion kit 2 used; post op. care (50Ǩl Anafen (1 mg/ml) and 500Ǩl saline (0.9%)); behavioral testing (Barnes maze test); In vivo transfection agent used: jetPEI; Dose (1.8Ǩg/day); Brain coordinates; ventricle A/P =−0.22 M/l = 0.0 D/v = −3.5.

16239 Schneider, J. S., Seyfried, T. N., Choi, H. S., Kidd, S. K. Intraventricular Sialidase Administration Enhances GM1 Ganglioside Expression and Is Partially Neuroprotective in a Mouse Model of Parkinson's Disease. PLoS One 2015; 10(-12-):e0143351. >>> Sialidase, vibrio cholera; CSF, artificial; CSF/CNS; mice; 2004; Controls received mp w/ vehicle; animal info (Male C57Bl6 mice, 7–10 weeks); ALZET brain infusion kit 3 used; good methods (pg. 3); post op. care (buprenorphine); immunology; brain tissue distribution; VCS aka Vibrio cholera sialidase; (aCSF recipe: 150mMNa, 3mMK, 1.4mMCa, 0.8mMMg, 1mMP and 155mMCl); anesthetized with ketamine/xylazine; Dose (0.25, 0.50, or 1.0U/mL); Brain coordinates; -0.22mm from Bregma, 0.87mm lateral to the midline, and 2.5mmbelow the cortical surface.

16226 Parsi, S., Smith, P. Y., Goupil, C., Dorval, V., Hebert, S. S. Preclinical Evaluation of miR-15/107 Family Members as Multifactorial Drug Targets for Alzheimer's Disease. Mol Ther Nucleic Acids 2015; 4(--):e256. >>> miR-16 mimic; Saline; CSF/CNS (ventricle); mice; 1007D; Controls received mp w/ vehicle; animal info (Wild-type mice (C57BL/6, female, 2 months old)); ALZET brain infusion kit 2 used; dose-response (pg. 2); neurodegenerative (Alzheimer’s); post op. care (Anafen); replacement therapy (miRNA replacement therapy); brain tissue distribution; delayed delivery (12 hours delivery/day); potent inhibitor of amyloid precursor protein (APP) and BACE1 expression, AǞpeptide production, and Tau phosphorylation in cells; Dose (4.2Ǩg/Ǩl); Brain coordinates; ventricle A/P =−0.22 M/L = 0.5 D/v = 3.5).

16215 Muelken, P., Schmidt, C. E., Shelley, D., Tally, L., Harris, A. C. A Two-Day Continuous Nicotine Infusion Is Sufficient to Demonstrate Nicotine Withdrawal in Rats as Measured Using Intracranial Self-Stimulation. PLoS One 2015; 10(-12-):e0144553. >>> Nicotine bitartrate; Saline, physiological; SC; rat; 2ML2; Controls received mp w/ vehicle; animal info (Male Wistar rats, 275–300 g); functionality of mp verified by elevations in intracranial self-stimulation; dose-response (pg 8, 9); post op. care (buprenorphine, ceftriaxone); behavioral testing (intracranial self-stimulation pg 4); Dependence, nicotine; “elevations in ICSS thresholds beginning on the second day ofinfusion” (Pg. 1); primed in saline for 1 hour prior to implantation; Dose (3.2 mg/kg/day);.

16211 Meschin, P., Demion, M., Cazorla, O., Finan, A., Thireau, J., Richard, S., Lacampagne, A. p11 modulates calcium handling through 5-HT(4)R pathway in rat ventricular cardiomyocytes. Cell Calcium 2015; 58(-6-):549-57. >>> Brain-derived neurotrophic factor; saline; SC; rat; 2ML2; Controls received mp w/ vehicle; animal info (200–224 g male Wistar rats); functionality of mp verified by ECG (electrocardiograms); post op. care (lidocaine, heating pad); replacement therapy (neurotrophin); Dose (180 ng/day));.

16204 Luo, D., Zhuang, X., Luo, C., Long, M., Deng, C., Liao, X., Wang, L. Continuous angiotensin-(1-7) infusion improves myocardial calcium transient and calcium transient alternans in ischemia-induced cardiac dysfunction rats. Biochem Biophys Res Commun 2015; 467(-4-):645-50. >>> Angiotensin (1-7); saline; IV (jugular); rat; 2ML4; Controls received mp w/ vehicle and sham operation; animal info: Sprague Dawley adult male rats, 180-220 g; functionality of mp verified by measurements of hemodynamic parameters, the calcium transient, and the heart rate threshold of calcium transient alternans; ischemia (cardiac); post op. care (IP injection of buprenorphine); cardiovascular; peptides;“evaluate impact of Ang-(1e7) on calcium transient (CaT) in cardiomyocytes during the pathogenesis of heart failure” (pg 645); Dose: 24 mg/kg per hour.

16172 Schaschkow, A., Mura, C., Dal, S., Langlois, A., Seyfritz, E., Sookhareea, C., Bietiger, W., Peronet, C., Jeandidier, N., Pinget, M., Sigrist, S., Maillard, E. Impact of the Type of Continuous Insulin Administration on Metabolism in a Diabetic Rat Model. J Diabetes Res 2016; 2016(--):8310516. >>> insulin; buffer solution; SC; rat; animal info (male, Lewis, STZ injection); comparison of injection of insulin vs mp; post op. care (Baytril 10 mg/kg QD for 7 days); diabetes; pumps primed for 24 hours in 37C saline;“…continuous insulin delivery by pumps restored normoglycaemia, which induced the reduction of both reactive oxygen species and macrophage infiltration into the liver and omentum. Injections controlled the glucose levels for only a short period of time and therefore tissue stress and inflammation were elevated.” pg 1; “pumps require no daily injection and facilitate rat follow- up. Well-being of the animals and the homogeneity of the results permit researchers to limit the numbers of animals and experiments used to build solid and reproducible results.” pg 8; Dose (4 IU/day);.

16171 Scarpace, P. J., Matheny, M., Strehler, K. Y., Toklu, H. Z., Kirichenko, N., Carter, C. S., Morgan, D., Tumer, N. Rapamycin Normalizes Serum Leptin by Alleviating Obesity and Reducing Leptin Synthesis in Aged Rats. J Gerontol A Biol Sci Med Sci 2016; 71(-7-):891-9. >>> Rapamycin; DMSO; PEG 400; CSF/CNS; Rat; Controls received mp w/ vehicle; animal info (male, F344 x Brown Norway, 24 months old); pumps replaced after 14 days; ALZET brain infusion kit used; 10% DMSO used; 90% PEG 400 used; post op. care (rats kept warm until recovered); used aCSF filled pump for 14 days, then replaced with rapamycin or vehicle for 28 day infusion; obesity; Dose (30 ug/day); Brain coordinates (1.3 mm posterior to bregma, 1.9 mm lateral to the midsaggital suture and to a depth of 3.5mm);.

16169 Sato, T., Pajarinen, J., Behn, A., Jiang, X., Lin, T. H., Loi, F., Yao, Z., Egashira, K., Yang, F., Goodman, S. B. The effect of local IL-4 delivery or CCL2 blockade on implant fixation and bone structural properties in a mouse model of wear particle induced osteolysis. J Biomed Mater Res A 2016; 104(-9-):2255-62. >>> Ultra-high molecular weight polyethylene particles; interleukin-4, mouse recombinant; BSA; PBS; Bone (femur); Mice; 2006; Controls received mp w/ vehicle; animal info (male, BALB/cByJ, 10-12 weeks old); 1% BSA used; post op. care (buprenorphine injection SC); used vinyl tubing to connect pumps to titanium rods;.

16162 Ramirez-Jarquin, U. N., Tapia, R. Neuropathological characterization of spinal motor neuron degeneration processes induced by acute and chronic excitotoxic stimulus in vivo. Neuroscience 2016; 331(--):78-90. >>> AMPA; Phosphate buffer; CSF/CNS (intrathecal); Rat; 2004; Controls received mp w/ vehicle; animal info (male, Wistar, 270-300g); neurodegenerative (amyotrophic lateral sclerosis); post op. care (IM penicillin); behavioral testing (rotarod, paw grip endurance; stride analysis); AMPA aka a-amino-3- hydroxy-5-methyl-4-isoxazoleacetic acid; pumps primed for 48 hours in 37C saline; used surgical clips;.

16152 Pereira, P. A., Rocha, J. P., Cardoso, A., Vilela, M., Sousa, S., Madeira, M. D. Effects of chronic alcohol consumption, withdrawal and nerve growth factor on neuropeptide Y expression and cholinergic innervation of the rat dentate hilus. Neurotoxicology 2016; 54(--):153-60. >>> Nerve growth factor; Methylene blue; BSA; CSF, artificial; CSF/CNS; Rat; 2002; animal info (male, Wistar); functionality of mp verified by residual volume; ALZET brain infusion kit used; post op. care (SC injections of 0.9% saline (2ml)); pulsed delivery; used PE-60 tubing; used lynch coil;.

16138 Nardelli, P., Vincent, J. A., Powers, R., Cope, T. C., Rich, M. M. Reduced motor neuron excitability is an important contributor to weakness in a rat model of sepsis. Exp Neurol 2016; 282(--):1-8. >>> Oxymorphine; IP; Rat; post op. care (buprenorphine SC 0.12 mg/kg; 5 ml SC saline; Baytril SC 10 mg/kg Q12H); used 2ML size; pumps used for continuous pain relief in rat sepsis model; Dose (30 ug/kg/h);.

16137 Hoegberg, B. G., Lomazzo, E., Lee, N. H., Perry, D. C. Regulation of alpha4beta2alpha5 nicotinic acetylcholinergic receptors in rat cerebral cortex in early and late adolescence: Sex differences in response to chronic nicotine. Neuropharmacology 2015; 99(--):347-55. >>> Nicotine hydrogen tartrate; Saline; SC; SC; 2002; Controls received mp w/ vehicle; animal info: Sprague Dawley rats; dose-response (pg 350); post op. care (buprenorphine); dependence; Dose: 17 mg/kg/day.

16127 Ghanbari, A., Esmaeilpour, T., Bahmanpour, S., Golmohammadi, M. G., Sharififar, S., Azari, H. Depletion of neural stem cells from the subventricular zone of adult mouse brain using cytosine b-Arabinofuranoside. Brain Behav 2015; 5(-11-):e00404. >>> Ara-C; Saline; CSF/CNS (right lateral ventricle); Mice; 1007D; Controls received sham operation and mp w/ vehicle; animal info: Five-week-old male C57-BL6 mice; pumps replaced once after 7 days; ALZET brain infusion kit 3 used; good methods (pg 2); post op. care (0.5 mg/kg buprenorphine every 12 h for 48 hours); cyanoacrylate adhesive (Loctite 454); delayed delivery (6-12 hours); Dose: 2% Ara-C in Saline; Brain coordinates; Paxinos mouse brain Atlas: Anterior-Posterior (AP): _0. 2, Medial-Lateral (ML): +0. 9 from the bregma point and Dorsal-Ventral: +2.7 from the top of skull.

16125 Gautier, H. O., Evans, K. A., Volbracht, K., James, R., Sitnikov, S., Lundgaard, I., James, F., Lao-Peregrin, C., Reynolds, R., Franklin, R. J., Karadottir, R. T. Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors. Nat Commun 2015; 6(--):8518. >>> Tetrodotoxin; Conotoxin, MVIIC w-; NBQX; Uridine, ethyldeoxy-; Saline; CSF/CNS; rat; 1004; Controls received mp w/ vehicle; animal info: Female Sprague–Dawley rats aged 10–14 weeks of age (190–250 g); Plastics One brain infusion kit used; good methods; neurodegenerative (demyelination); post op. care (buprenorphine); cyanoacrylate adhesive (pg 12); delayed delivery (3 dpl and 10 dpl), using corn oil to separate drug from saline in a catheter; “The onset of drug delivery into the lesion was controlled by varying the length of the tube that connects the minipump to the cannula and by inserting a sterile corn oil drop (Sigma) to prevent diffusion of the drug solution into the initial saline solution” (pg 12); Dose: 11 mM conotoxin; 50nM tetrodotoxin; 250 uM NBQX; 5 ug/ul EdU.

16120 Ferrón, Sacri R., Andreu-Agulló, Celia, Mira, Helena, Sánchez, Pilar, Ángeles Marqués-Torrejón, M., Fariñas, Isabel  Nature Protocols 2007; 2(-4-):849-859. >>> Growth Factor; Saline; CSF/CNS (ventricle); mice; 1007D; animal info: any; ALZET brain infusion kit 3 used; good methods (pg 854); post op. care (mice placed on thermal blanket); brain tissue distribution; Cannula placement verified via tissue distribution upon brain removal; Brain coordinates; 0.0 antero-posterior, 0.7 medio-lateral stereotaxic coordinates.

16114 Dudek, M., Abo-Ramadan, U., Hermann, D., Brown, M., Canals, S., Sommer, W. H., Hyytia, P. Brain activation induced by voluntary alcohol and saccharin drinking in rats assessed with manganese-enhanced magnetic resonance imaging. Addict Biol 2015; 20(-6-):1012-21. >>> Manganese Chloride; Saline, Trist-buffered; SC; rat; 2001; animal info: male alcohol-preferring AA rats, 265–335 g; post op. care (subcutaneous injection of carprofen); MRI done after infusion; brain tissue distribution; brain Mn2+ accumulation; Dose: 120 mg/kg/week.

16111 Dabrowski, W., Kwiecien, J. M., Rola, R., Klapec, M., Stanisz, G. J., Kotlinska-Hasiec, E., Oakden, W., Janik, R., Coote, M., Frey, B. N., Turski, W. A. Prolonged Subdural Infusion of Kynurenic Acid Is Associated with Dose-Dependent Myelin Damage in the Rat Spinal Cord. PLoS One 2015; 10(-11-):e0142598. >>> Kynurenic Acid; PBS; CSF/CNS (intrathecal); rat; 2ML1; Controls received mp w/ vehicle; animal info: 42 healthy adult rats; spinal cord injury; post op. care (ketoprofen); Rat intrathecal catheter used; Dose: 0.0002 pmol/min, 0.01 nmol/min, 0.1 nmol/min, 1 nmol/min and 10 nmol/min of Kynurenic acid.

16096 Bernardi, S., Toffoli, B., Zennaro, C., Bossi, F., Losurdo, P., Michelli, A., Carretta, R., Mulatero, P., Fallo, F., Veglio, F., Fabris, B. Aldosterone effects on glomerular structure and function. J Renin Angiotensin Aldosterone Syst 2015; 16(-4-):730-8. >>> Aldosterone; Eplerenone; NaCl; SC; rat; 2004; 2ML4; Controls received mp w/ vehicle, Harlan Teklad 18% Protein Global Diet; animal info: wild-type male Wistar rats, weighing 250 g; functionality of mp verified by: systolic blood pressure (SBP) via tail cuff plethysmography; post op. care (buprenorphine); injected SC 0.05 mg/kg day of; 0.025 mg/kg day after; cardiovascular; cardiovascular; Dose: aldosterone -- 72Ǩg/kg/day; eplerenone -- 100 mg/kg/day.

16077 Zendedel, A., Johann, S., Mehrabi, S., Joghataei, M. T., Hassanzadeh, G., Kipp, M., Beyer, C. Activation and Regulation of NLRP3 Inflammasome by Intrathecal Application of SDF-1a in a Spinal Cord Injury Model. Mol Neurobiol 2016; 53(-5-):3063-75. >>> Stromal cell-derived factor-1, alpha; PBS; BSA; CSF/CNS (intrathecal); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Wistar, 14 weeks old, 350-400g); 1% BSA used; post op. care (saline rehydration; baytril 0.3 ml SC BID; manual bladder emptying BID); behavioral testing (BBB locomotor test); immunology;.

16075 Yi, Q. Y., Qi, J., Yu, X. J., Li, H. B., Zhang, Y., Su, Q., Shi, T., Zhang, D. M., Guo, J., Feng, Z. P., Wang, M. L., Zhu, G. Q., Liu, J. J., Shi, X. L., Kang, Y. M. Paraventricular Nucleus Infusion of Epigallocatechin-3-O-Gallate Improves Renovascular Hypertension. Cardiovasc Toxicol 2016; 16(-3-):276-85. >>> gallate, Epigallocatechin-3-O; CSF, artificial; CSF/CNS (paraventricular nucleus); Rat; 1004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-275g); bilateral cannula used; post op. care (buprenorphine 0.01 mg/kg SC 12 hours post); tissue perfusion (paraventricular nucleus); tissue perfusion (paraventricular nucleus); tissue perfusion (paraventricular nucleus); Dose (20 ug/h); Brain coordinates“1.8 mm posterior, 0.4 mm lateral to the bregma and 7.9 mm ventral to the zero level“ pg 277;.

16068 Wu, K. L., Wu, C. W., Chao, Y. M., Hung, C. Y., Chan, J. Y. Impaired Nrf2 regulation of mitochondrial biogenesis in rostral ventrolateral medulla on hypertension induced by systemic inflammation. Free Radic Biol Med 2016; 97(--):58-74. >>> Endotoxin, LPS; coenzyme Q10; temple; Interleukin-1, receptor antagonist; butylhydroquinone, tert-; Saline; CSF, artificial; IP; CSF/CNS (cisterna magna); Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 8 weeks old, 250-280g); ALZET brain infusion kit 2 used; post op. care (IM procaine Penicillin 1000 IU); tissue perfusion (cisterna magna); cardiovascular; bp measured using tail cuff; Dose (LPS 1.2 mg/kg/day; coenzyme Q10 3.25 ug/ul/hr; tempol 5 ug/ul/hr; IL-1Ra 0.5 ug/ul/h);.

16043 Tighilet, Brahim, Dutheil, Sophie, Siponen, Marina I., Noreña, Arnaud J. Reactive Neurogenesis and Down-Regulation of the Potassium-Chloride Cotransporter KCC2 in the Cochlear Nuclei after Cochlear Deafferentation. Frontiers in Pharmacology 2016; 7(--):. >>> AraC; muscimol; NaCl; CSF, artificial; CSF/CNS (fourth ventricle); Cat; 2ML4; Controls received mp w/ vehicle; animal info (male, adult, 4-5kg); post op. care (antibiotics for 7 days; analgesic for 3 days);.

16033 Rakesh Kumar,, K. H. Reeta,, Subrata Basu Ray  Indian Journal of Experimental Biology 2014; 52(--):317-322. >>> loperamide; morphine; PEG, saline, ethanol; CSF/CNS (intrathecal); rat; 2ML2; Controls received mp w/ vehicle; animal info (adult male Sprague-dawley rats, 275-350 g); dose-response (pg 319, 320); good methods (pg. 317-318); post op. care (neomycin ointment and perioperative ketoprofen [3 mg/kg, im] given); behavioral testing (stepping placing, righting reflex, and Hargreaves paw withdrawal test); delayed delivery (lynch coil filled with saline used to delay administration of drug by 3 days; ); Dose: Morphine and Loperamide ( 0.5,1, and 2 uL/hr).

16027 Hussmann, G. P., DeDominicis, K. E., Turner, J. R., Yasuda, R. P., Klehm, J., Forcelli, P. A., Xiao, Y., Richardson, J. R., Sahibzada, N., Wolfe, B. B., Lindstrom, J., Blendy, J. A., Kellar, K. J. Chronic sazetidine-A maintains anxiolytic effects and slower weight gain following chronic nicotine without maintaining increased density of nicotinic receptors in rodent brain. J Neurochem 2014; 129(-4-):721-31. >>> nicotine; sazetidine-A; varenicline; saline; SC; rat, mice; Controls received mp w/ saline vehicle; animal info (male adult Sprague-dawley rats, 8 weeks, 240 g; male 129SvJ;C57BL/6J F1 hybrid mice, 6 weeks, 25-30 g ); functionality of mp verified by protein binding in rat cortex ; pumps replaced after 2 weeks; 1 additional week of infusion for mice, 4 additional weeks of infusion for rats; post op. care (buprenorphine or ketoprofen injected for analgesia); behavioral testing (memory retention and attention in rats); Saz-A aka sazetidine-A; Rats Dose: 6 mg/kg/day nicotine, 4.7 mg/kg/day saz-A, 1.2 mg/kg/day vareniclineMice Dose: 18 mg/kg/day nicotine, 1.8 mg/kg/day saz-A, 1.8 mg/kg/day varenicline.

16013 Ibrahim-Zada, I., Rhee, P., Gomez, C. T., Weller, J., Friese, R. S. Inhibition of sepsis-induced inflammatory response by beta1-adrenergic antagonists. J Trauma Acute Care Surg 2014; 76(-2-):320-7; discussion 327-8. >>> Esmolol; saline; IV (jugular); mice; Controls received mp w/ saline vehicle; animal info (Male C57BL/6J mice aged 8 weeks to 12 weeks); functionality of mp verified by monitoring of animal response using transthoraic chocardiography; dose-response (pg 321, 322 dose response curve); good methods (pg 321); post op. care (buprenorphine 0.025 mg in 1 mL of normal saline injected SC); observation of animals every 12 hours; Dose: emsolol 6.7 ug/kg/min (pg. 321) [10mg/mL];.

16010 Fallowfield, J. A., Hayden, A. L., Snowdon, V. K., Aucott, R. L., Stutchfield, B. M., Mole, D. J., Pellicoro, A., Gordon-Walker, T. T., Henke, A., Schrader, J., Trivedi, P. J., Princivalle, M., Forbes, S. J., Collins, J. E., Iredale, J. P. Relaxin modulates human and rat hepatic myofibroblast function and ameliorates portal hypertension in vivo. Hepatology 2014; 59(-4-):1492-504. >>> H2-Relaxin, human recombinant; Sodium acetate; SC; rat; 2ML2; Controls received mp w/ vehicle; minipumps were loaded with either agent or vehicle solution; animal info ( Sprague-dawley (250-300g)); functionality of mp verified by measuring blood levels from the inferior vena cava or portal vein ; dose-response (full dose response curve present; systemic blood levels); post op. care (warmed and received 0.9% NaCl at 10mL/kg/hr); gene therapy; RLN aka Relaxin; dose: (.5 mg/kg/day);.

16003 Plato Mak,, Jillian H. Broadbear,, Anton Kolosov,, Colin S. Goodchild,  PAIN MEDICINE 2015; 16(--):1781-1793. >>> Ketamine; morphine; SC; Rat; 2ML1; Controls received dummy implant; animal info (male, Wistar); post op. care (carpofen IP injection); behavioral testing (nociception, rotarod, open field); diabetes; pumps removed after 5 days; Dose (20 mg/kg/day);.

16002 Madularu, D., Kulkarni, P., Ferris, C. F., Brake, W. G. Changes in brain volume in response to estradiol levels, amphetamine sensitization and haloperidol treatment in awake female rats. Brain Res 2015; 1618(--):100-10. >>> Haloperidol; SC; Rat; 2002; Controls received sham surgery; animal info (female, Sprague Dawley, 200-250g 2-3 months old, OVX); post op. care (Anafen 0.1 mL/rat; antibiotic ointment); MRI; PEEK; Dose (0.25 mg/kg/day);.

15998 Honjo, T., Chyu, K. Y., Dimayuga, P. C., Lio, W. M., Yano, J., Trinidad, P., Zhao, X., Zhou, J., Cercek, B., Shah, P. K. Immunization with an ApoB-100 Related Peptide Vaccine Attenuates Angiotensin-II Induced Hypertension and Renal Fibrosis in Mice. PLoS One 2015; 10(-6-):e0131731. >>> Angiotensin II; Saline; SC; Mice; 2004; Controls received mp w/ vehicle; animal info (male, apoE -/-, 10 weeks old); post op. care (SC analgesic); cardiovascular; immunology; peptides; Dose (1000 ng/kg/min);.

15997 Uli S. Herrmann,, Anne K. Schütz,, Hamid Shirani,, Danzhi Huang,, Dino Saban, Mario Nuvolone,, Bei Li,, Boris Ballmer,, Andreas K. O.Åslund,, Jeffrey J. Mason,, Elisabeth Rushing,, Herbert Budka,, Sofie Nyström,, Per Hammarström,, Anja Böckmann,, Amedeo Caflisch,, Beat H. Meier,, K. Peter R. Nilsson,, Simone Hornemann,, Adriano Aguzzi  Science Translational Medicine 2015; 7(-299-):1-18. >>> LIN1001l; LIN5001; LIN5002; LIN7002; PBS; CSF/CNS; Mice; 2006; Controls received mp w/ vehicle; animal info (WT CD1 or PrP-overexpressing mice); pumps replaced every 42 days; post op. care (Finadyne 2.5 mg/kg BID SC; Temgesic SC injection 0.1 mg/kg; Borgal 2ml 24% in drinking water); post op. care (Finadyne 2.5 mg/kg BID SC; Temgesic SC injection 0.1 mg/kg; Borgal 2ml 24% in drinking water); long-term study; Dose (3.3 mg/kg/week); brain coordinates;.

15967 Iijima, M., Koike, H., Chaki, S. Effect of an mGlu2/3 receptor antagonist on depressive behavior induced by withdrawal from chronic treatment with methamphetamine. Behav Brain Res 2013; 246(--):24-8. >>> methamphetamine (MAP); saline; SC; rat; 2ML1; animal info: male, Sprague-Dawley, 5 wks old; tolerance studies; post op care: pumps removed under sodium pentobarbital anesthesia; behavioral testing: forced swimming test, locomotor activity; mp used to infuse methamphetamine to induce a withdrawal-like effect in rats to study the effect of LY341495 (mGlu2/3 receptor antagonist) on withdrawal-induced depressive behavior; dose 2, 5 mg/kg/day.

15960 Li, H. B., Qin, D. N., Cheng, K., Su, Q., Miao, Y. W., Guo, J., Zhang, M., Zhu, G. Q., Kang, Y. M. Central blockade of salusin beta attenuates hypertension and hypothalamic inflammation in spontaneously hypertensive rats. Sci Rep 2015; 5(--):11162. >>> Immunoglobulin G, antisalusin b; CSF/CNS (paraventricular nucleus); Rat; 1004; Controls received mp w/ vehicle and control antibody; animal info (male, Wistar Kyoto, SHR, 10 weeks old); bilateral cannula used; post op. care (buprenorphine SC); tissue perfusion (paraventricular nucleus); cardiovascular; Cannula placement verified via histological analysis; bilateral infusion; bp measured using tail cuff; Dose (50, 100, 150 ng/kg/day);.

15925 Phie, J., Haleagrahara, N., Newton, P., Constantinoiu, C., Sarnyai, Z., Chilton, L., Kinobe, R. Prolonged Subcutaneous Administration of Oxytocin Accelerates Angiotensin II-Induced Hypertension and Renal Damage in Male Rats. PLoS One 2015; 10(-9-):e0138048. >>> Angiotensin II; oxytocin; PBS; SC; Rat; 2004; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 8-9 weeks old, 240-350g); post op. care (swabbed with 7.5% w/v iodine); cardiovascular; peptides; Dose (Ang II 40 ug/kg/h; OT 20 or 100 ng/kg/h);.

15913 Karalija, A., Novikova, L. N., Kingham, P. J., Wiberg, M., Novikov, L. N. The effects of N-acetyl-cysteine and acetyl-L-carnitine on neural survival, neuroinflammation and regeneration following spinal cord injury. Neuroscience 2014; 269(--):143-51. >>> L- N-acetyl-cysteine; O-acetyl-L-canitine; saline; Intrathecal; rats; 2002; Controls received mp w/ vehicle; animal info (adult, 10-12 week female Sprague-Dawley rats); pumps replaced every 2 weeks; spinal cord injury; stab wound in dorsal root entry zone of C3 spinal cervical segment; post op. care (given analgesic Finadyne, normal saline, and benzylpenicillin; each animal housed alone); Peptides ALC, NAC; NAC aka N-Acetyl-cysteine; ALC aka acetyl-L-carnitine; Dose: 2.4 mg/day for NAC, 0.9 mg/day for ALC.

15905 Flora, G., Joseph, G., Patel, S., Singh, A., Bleicher, D., Barakat, D. J., Louro, J., Fenton, S., Garg, M., Bunge, M. B., Pearse, D. D. Combining neurotrophin-transduced schwann cells and rolipram to promote functional recovery from subacute spinal cord injury. Cell Transplant 2013; 22(-12-):2203-17. >>> Rolipram; Saline; ethanol; SC; Rat; 2001; animal info (female, Fischer, adult, 180-200g); pumps replaced every week; pumps replaced every week; spinal cord injury; Multiple pumps per animal (2); stress/adverse reaction: (see pg.2205); post op. care (gentamicin 5 mg/kg IM QD for 7 days; Buprenex 0.3 mg/kg SC QD for 2 days; lactated ringer's solution 5ml BID PRN, bladders expressed BID); Dose (0.5 mg/kg);.

15895 Wang, Y., Wang, L., Zhang, F., Zhang, C., Deng, S., Wang, R., Zhang, Y., Huang, D., Huang, K. Inhibition of PARP prevents angiotensin II-induced aortic fibrosis in rats. Int J Cardiol 2013; 167(-5-):2285-93. >>> Angiotensin II; saline,; SC; rat; 2002; controls received mp w/ vehicle; sprague-Dawley, 150-300 g, male ; functionality of mp verified by Systolic blood pressure measured by tail-cuff method (pg.2286); post op. care (50 mg/kg pentobarbital); dose: 120 ng/kg/min.

15893 Vieira, F. G., Ping, Q., Moreno, A. J., Kidd, J. D., Thompson, K., Jiang, B., Lincecum, J. M., Wang, M. Z., De Zutter, G. S., Tassinari, V. R., Levine, B., Hatzipetros, T., Gill, A., Perrin, S. Guanabenz Treatment Accelerates Disease in a Mutant SOD1 Mouse Model of ALS. PLoS One 2015; 10(-8-):e0135570. >>> Guanabenz acetate; Ethanol; water; propylene glycol; SC; Mice; 2004; Controls received mp w/ vehicle; animal info (SOD1-G93A); pumps replaced every 28 days; dose-response (pg 4); neurodegenerative (amyotrophic lateral sclerosis); post op. care (antibiotic ointment; buprenorphine 0.1 mg/kg); long-term study; used lot#10284-12; Dose (0.45, 1.5, or 4.5 mg/kg/day);.

15892 Guvenc Tuna, B., Lachkar, N., de Vos, J., Bakker, E. N., VanBavel, E. Cerebral Artery Remodeling in Rodent Models of Subarachnoid Hemorrhage. J Vasc Res 2015; 52(-2-):103-15. >>> L682.777; PBS; DMSO; IP; Rat; 1003D; Controls received mp w/ vehicle; animal info (male, Wistar, 8-12 weeks old); 9% DMSO used; post op. care (buprenorphine/temgesic); cardiovascular; enzyme inhibitor (transglutaminase);.

15881 Wu, D., Klaw, M. C., Connors, T., Kholodilov, N., Burke, R. E., Tom, V. J. Expressing Constitutively Active Rheb in Adult Neurons after a Complete Spinal Cord Injury Enhances Axonal Regeneration beyond a Chondroitinase-Treated Glial Scar. J Neurosci 2015; 35(-31-):11068-80. >>> Chondroitinase ABC; PBS; trehalos; CSF/CNS (intrathecal); Rat; 2002; Controls received mp w/ vehicle; animal info (female, Wistar, adult, 225-250g); spinal cord injury; post op. care (buprenorphine, cefazolin, Lactated Ringer's solution, bladders manually expressed BID);"Because ChABC alone is thermolabile, we mixed it with the sugar trehalos to thermostabilize it"pg 11069; pumps primed in 37C saline evernight;.

15835 Kathryn R. Walsh, Jill T. Kuwabara, Joon W. Shim, and Richard D. Wainford  Am J Physiol Regul Integr Comp Physiol 2016; 310(--):R115-R124. >>> Norepinephrine; hydrochlorothiazide; losartan; Saline; DMSO; SC; Rat; 2ML2; Controls received mp w/ vehicle; animal info (male, Sprague Dalwey, 275-299g); post op. care (penicillin 0.3 ml IM 300,000 units/ml); cardiovascular; Dose (losartan 3 mg/kg/day, NE 600 ng/min; HCTZ 4 mg/kg/day);.

15831 John P. Valenta and Rueben A. Gonzales  ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH 2016; 40(-1-):. >>> Monocyte chemoattractant protein-1, recombinant rat; CSF, artificial; water, distilled; albumin, rat serum; CSF/CNS; Rat; 1004; Controls received mp w/ vehicle; animal info (male, Long Evans, 350g); functionality of mp verified by ELISA and manufacturers instructions see pg189; ALZET brain infusion kit 2 used; dose-response (pg.190); post op. care (bupivicane intradermally; bupivacaine and gentamicin dripped into wound); behavioral testing (alcohol self administration); stability verified by (ELISA pg 192); cyanoacrylate adhesive;“We chose the ICV method to simulate the presence of brain-induced MCP-1 and the method’s ability to target the whole brain with molecules that do not readily cross the BBB” pg 188-189; pumps primed at 37C sterile saline; Dose (0.2, 20, 2000 ng/day);.

15825 Veronique St-Onge, AlexanderWatts, Alfonso Abizaid  Horm. Behav 2016; 78(--):141-149. >>> Ghrelin; growth hormone-releasing peptide 6, [D-Lys-3]; Saline; CSF/CNS (ventral tegmental area); Rat; 2002; Controls received mp w/ vehicle; animal info (male, Long evans, 216-375g); post op. care (SC injection of meloxicam; feed of mashed food); behavioral testing (food operant responses); used Plastics One cannula; obesity;.

15822 Yue Shen,, Fang Cheng,, Mehul Sharma,, Yulia Merkulova,, Sheetal A. Raithatha,, Leigh G. Parkinson,, Hongyan Zhao,, Kathryn Westendorf,, Lubos Bohunek,, Tatjana Bozin, Ivy Hsu,, Lisa S. Ang, Sarah J. Williams,, R. Chris Bleackle, John E. Eriksson,, Michael A. Seidman,, Bruce M. McManus,, David J. Granville  Am J Pathol 2016; 186(--):87-100. >>> Angiotensin II; SC; Mice; 1004; Controls received mp w/ saline; animal info (male, C57BL6J, 3-4 months old); post op. care (SC buprenorphine injection); cardiovascular; peptides; pumps primed 24 hours in 37C saline; bp measured using tail cuff; Dose (1000 ng/kg/min);.

15817 Eduardo H. Sanchez-Mendoza, Jeismar Carballo, Marines Longart, Dirk M. Hermann, Thorsten R. Doeppner  Journal of Visualized Experiments 2016; 107(--):1-9. >>> Erythropoietin, recombinant human; CSF/CNS; Mice; Controls received mp w/ vehicle; animal info (C57BL6); good methods (Jove Video; picture of pump and implantation pg. 4); ischemia (cerebral); post op. care (Carprofen 4 mg/kg); behavioral testing (rotarod test; hand grip strength); cyanoacrylate adhesive;“In this work we have shown the method of implantation of minipumps with a cannula connected to the skull in order to deliver the plasticity promoting protein rhEpo directly into the ventricle, thus circumventing the BBB.” pg 8; Cannula placement verified via histologic analysis"The are no evident severe tissue alterations based on Nissl staining as compared to the corresponding contralateral area";.

15813 Regina R. Reimann, Tiziana Sonati, Simone Hornemann, Uli S. Herrmann, Michael Arand, Simon Hawke, Adriano Aguzzi  PLoS Pathog 2016; 12(-1-):1-19. >>> Antibody, 31C6; antibody, POM1; PBS; CSF/CNS; Mice; 2004; animal info (BL6.129-Prnp); post op. care (SC injections of buprenorphinum, funixin, and 5% glucose; Sulfadoxinum and sugar were added into water for 1 week post op); MRI; pumps primed in 37C PBS for 24 hours; used PEEK tubing;.

15780 Brigitte Koenig, Daniel Pape, Owen Chao, Jordana Bauer, Barbara Grimpe  Experimental Neurology 2016; 276(--):51-58. >>> DNAXT-1as; CSF/CNS (intrathecal); Rat; 1007D; Controls received mp w/ control enzyme or PBS; animal info (female, Wistar, 200-250g); pumps replaced every week; pumps replaced every week; spinal cord injury; post op. care (Baytril; manual bladder emptying; Rimadyl); behavioral testing (horizontal ladder task); used self-made intrathecal catheter from 32-gauge polyurethane; enzyme inhibitor (DNA enzyme of mRNA of xylosyltransferase-1);.

15769 Achikam Haima,, Christopher Albin-Brooks, Morgan Sherer, Emily Mills, Benedetta Leuner  Hormones and Behavior 2016; 77(--):124-131. >>> Citalopram HBr; Saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, GD); functionality of mp verified by residual volume; post op. care (ibuprofen 15 mg/kg in drinking water for 7 days); post op. care (ibuprofen 15 mg/kg in drinking water for 7 days); teratology; Therapeutic indication (post partum stress); Dose (10 mg/kg/day);.

15763 Meriem Gaval-Cruz1, Richard B. Goertz,, Daniel J. Puttick, Dawn E. Bowles, Rebecca C. Meyer, Randy A. Hall, Daijin Ko, Carlos A. Paladini, David Weinshenker  Addiction Biology 2016; 21(--):35-48. >>> Nepicastat; Saline; DMSO; IP; Mice; 2004; Controls received mp w/ vehicle; Controls received mp w/ vehicle; 50% DMSO used; post op. care (buprenorphine 2.5 mg/kg SC); behavioral testing (novelty induced locomotion); pumps primed in 37C saline 1 day before; pumps primed in 37C saline 1 day before;.

15760 Eric E. Ewan, Theo Hagg  journal of Neurotrauma 2016; 33(--):269-277. >>> Carnitine, acetyl-L-; PBS; CSF/CNS (intrathecal); Rat; 2001; animal info (female, Sprague Dawley, 200g); spinal cord injury; post op. care (wound rinced in steril saline, bacitracin applied topically, gentamicin IM 0, 2 or 4 days later, buprenorphine IM every 12 hours, bladders manually expressed BID); behavioral testing (locomotor function, grid walking, open field); pumps primed overnight; pumps removed after 6 days; Dose (1 mg/day);.

15759 LEIGH J. ELLMERS, PhD, MIRIAM T. RADEMAKER, PhD, CHRISTOPHER J. CHARLES, PhD,, TIM G. YANDLE, PhD, AND A. MARK RICHARDS, MD, PhD  JOURNAL OF CARDIAC FAILURE 2016; 22(-1-):64-72. >>> Handle region peptide, mouse; Saline; SC; Mice; 1004; Controls received mp w/ vehicle; animal info (male, C57BL5, 12 week sold); post op. care (SC buprenorphine 0.1 mg/kg/day for 2 days); cardiovascular; peptides; HRP is a prorenin receptor antagonist; bp measured using tail cuff; Dose (0.1 mg/kg/day);.

15758 Daniel G. Donner,, Grace E. Elliott,, Belinda R. Beck,, Andrew C. Bulmer,, Alfred K. Lam,, John P. Headrick,, and Eugene F. Du Toit  Endocrinology 2016; 157(-1-):368-381. >>> Testosterone; trenbolone; Cyclodextrin, 2b-hydroxypropyl; SC; Rat; 2004; Controls received mp w/ vehicle; animal info (male, Wistar, 12 weeks old, 300g); functionality of mp verified by plasma; pumps replaced every 4 weeks; 45% cyclodextrin used; ischemia (cardiac); post op. care (buprenorphine 10 ug/kg/day IM; enrofloxacin 5 mg/kg ip for 3 days); long-term study; Dose (2 mg/kg/day);.

15752 Luis M. Craveiro,, Oliver Weinmann,, Bernd Roschitzki,, Roman R. Gonzenbach,, Björn Zörner,, Laura Montani,, Benjamin K. Yee,, Joram Feldon,, RomanWilli,, Martin E. Schwaba,  Experimental Neurology 2013; (-250-):52-68. >>> Antibody, anti-Nogo-A 11C7; PBS; CSF/CNS (intrathecal); Rat; 2ML2; 2ML4; Controls received mp w/ control antibody or PBS; animal info (male, Long Evans, 2-3 months old, 300g); post op. care (Rimadyl 5 mg/kg injection SC, Baytril 5 mg/kg injection SC for 72 hours at 12 hour intervals); behavioral testing (locomotor activity, anxiety, open field test, elevated plus maze); toxicology;“This procedure ensured antibody delivery into the CSF at a constant flow rate for 2 or 4 weeks.” pg 53;.

15724 Sara K.S. Bengtsson,, Maja Johansson,, Torbjörn Bäckström  Horm. Behav 2016; (-78-):160-167. >>> Allopregnanolone; Captisol; SC; Mice; 1004; Controls received mp w/ vehicle; animal info (C57BL6J); pumps replaced every month; 60% Captisol used; post op. care (Carprofen); behavioral testing (Morris water maze); long-term study; Industry authored (Umecrine Cognition AB);.

15710 Jonathan A. Fallowfield,, Annette L. Hayden,, Victoria K. Snowdon,, Rebecca L. Aucott,, Ben M. Stutchfield,, Damian J. Mole,, Antonella Pellicoro,, Timothy T. Gordon-Walker,, Alexander Henke,, Joerg Schrader,, Palak J. Trivedi,, Marc Princivalle,, Stuart J. Forbes,, Jane E. Collins,, and John P. Iredale  Hepatology 2014; 59(-4-):1492-1504. >>> Recombinant human H2-RLN; Sodium acetate; SC; rat; 2ML2; Controls received mp w/ vehicle; sodium acetate pH 5; minipumps were loaded with either agent or vehicle solution; animal info ( Sprague-dawley, 250-300g); functionality of mp verified by plasma level in inferior vena cava or portal vein; dose-response (full dose response curve present; systemic blood levels); post op. care (warmed and received 0.9% NaCl at 10mL/kg/hr); gene therapy; RLN aka Relaxin; dose: (.5 mg/kg/day).

15236 Sonsalla, P.K., Coleman, C., Wong, L.Y., Harris, S.L., Richardson, J.R., Gadad, B.S., Li, W.H., German, D.C. The angiotensin converting enzyme inhibitor captopril protects nigrostriatal dopamine neurons in animal models of parkinsonism. EXPERIMENTAL NEUROLOGY 2013; 250(-;-):376-383. >>> MPP+iodide; Saline, sterile; CSF/CNS; Rat; 2ML4; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 300g); neurodegenerative (Parkinson's disease); post op. care (rimadyl 5 mg/kg SC, ampacillin 100 mg/kg IM);.

15233 Sharp, K.G., Yee, K.M., Stiles, T.L., Aguilar, R.M., Steward, O. A re-assessment of the effects of treatment with a non-steroidal anti-inflammatory (ibuprofen) on promoting axon regeneration via RhoA inhibition after spinal cord injury. EXPERIMENTAL NEUROLOGY 2013; 248(-;-):321-337. >>> Ibuprofen; Saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 9-10 weeks old); spinal cord injury; post op. care (bladders expressed BID); behavioral testing (hindlimb locomotor function; gridwalk task, footprint analysis, girdle test, plantar von frey); immunology; pumps primed primed for 16h in 37C saline;.

15215 Hawryluk, G.W.J., Spano, S., Chew, D., Wang, S., Erwin, M., Chamankhah, M., Forgione, N., Fehlings, M.G. An Examination of the Mechanisms by Which Neural Precursors Augment Recovery Following Spinal Cord Injury: A Key Role for Remyelination. CELL TRANSPLANTATION 2014; 23(-3-):365-380. >>> Epithelial growth factor; fibroblast growth factor; platelet-derived growth factor; CSF/CNS (intrathecal); Rat; 1007D; Animal info (female, Wistar, 10-12 weeks old); spinal cord injury; post op. care (bladders manually evacuated TID, Minocycline 50 mg/kg 10 days, cyclosporine 10 mg/kg); behavioral testing (open field test); immunology;.

15209 Dhar, I., Dhar, A., Wu, L.Y., Desai, K.M. Methylglyoxal, a Reactive Glucose Metabolite, Increases Renin Angiotensin Aldosterone and Blood Pressure in Male Sprague-Dawley Rats. AMERICAN JOURNAL OF HYPERTENSION 2014; 27(-3-):308-316. >>> Methylglyoxal; SC; Rat; 2ML4; Controls received mp w/ saline, normal; animal info (male, Sprague Dawley, 12 weeks old); post op. care (buprenorphine 0.025 mg/kg SC BID for 2 days after surgery); cardiovascular; bp measured using catheter;.

15205 Claflin, D.I., Greenfield, L.R., Hennessy, M.B. Modest elevation of corticosterone in preweanling rats impairs subsequent trace eyeblink conditioning during the juvenile period. BEHAVIOURAL BRAIN RESEARCH 2014; 258(-;-):19-26. >>> Corticosterone; PEG 400; SC; Rat; 1003D; Controls received mp w/ vehicle; animal info (Long-Evans, PND15); functionality of mp verified by plasma levels pg22; no stress"Animals recovered quickly..."(see pg.20); post op. care (antibiotic ointment on wound, buprenorphine (0.052 mg/kg, heating pad until responsive and then returned to dam);“The corticosterone pellet was designed to produce a low, constant release of hormoneover a 21-day period, but plasma assays showed otherwise. There was a large supraphysiological increase in circulating corticoste-rone levels (to ∼80 ug/dl) for about three days after implantationand a return tonormal levels by the time testing occurred...In the present study, we administered cor-ticosterone using an alternative, more reliable method that yielded the low level (within a normal physiological range) and constantrate of delivery we had originally expected. Furthermore, since the∼3-day period of elevation in the previous study was sufficient toproduce lasting effects on behavior, we chose to use an osmotic mini-pump that was designed to deliver corticosterone at a lowand constant rate over a 3-day period only. ” pg 20; pumps primed for 24 hours in sterile saline;.

15183 Oosterhof, C.A., El Mansari, M., Blier, P., Oosterhof, C.A. Asenapine alters the activity of monoaminergic systems following its subacute and long-term administration: An in vivo electrophysiological characterization. EUROPEAN NEUROPSYCHOPHARMACOLOGY 2015; 25(--):531-543. >>> Asenapine; Saline; SC; Rat; Controls received mp w/ vehicle; animal info (male, Sprague Dalwley, 300-450g); post op. care (Temgesic 0.1 mg/kg SC);.

15142 Williams, B.A., Butt, M.T., Zeller, J.R., Coffee, S., Pippi, M.A., Williams, B.A. Multimodal Perineural Analgesia with Combined Bupivacaine-Clonidine-Buprenorphine-Dexamethasone: Safe In Vivo and Chemically Compatible in Solution. PAIN MEDICINE 2015; 16(--):186-198. >>> Bupivacaine; clonidine; dexamethasone; Saline; CSF/CNS (sciatic nerve); Rat; 2ML1; Controls received mp w/ vehicle; animal info (male, albino, CD[SD]); no stress (see pg. 192); post op. care (IM butorphanol tartrate 0.05 mg/kg, ceftiofur sodium 5 mg/kg); stability verified by (pg. 195); used polyurethane catheter 0.5mm ID 0.9 mmOD; pumps removed after 1 week; dose (66.6 ug/mL).

15129 Wang, J., Wang, X.F., Wei, J., Wang, M.Y., Wang, M.Y. Hyaluronan Tetrasaccharide Exerts Neuroprotective Effect and Promotes Functional Recovery After Acute Spinal Cord Injury in Rats. NEUROCHEMICAL RESEARCH 2015; 40(--):98-108. >>> Hyaluronan tetrasaccharide; CSF/CNS (intrathecal); Rat; Controls received mp w/ vehicle; animal info (Sprague Dawley, 12 weeks old, 250-300g); spinal cord injury; post op. care (Baytril antibacterial applied SC for 3 days); behavioral testing (locomotor activity); immunology;.

15121 Trivedi, C., Shan, X.Y., Tung, Y.C.L., Kabra, D., Holland, J., Amburgy, S., Heppner, K., Kirchner, H., Yeo, G.S.H., Perez-Tilve, D., Perez-Tilve, D. Tachykinin-1 in the Central Nervous System Regulates Adiposity in Rodents. ENDOCRINOLOGY 2015; 156(--):1714-1723. >>> Ghrelin; neuropeptide K; Saline; CSF/CNS (fourth ventricle); CSF/CNS; Rat; mice; 1002;1007D; Controls received mp w/ vehicle; animal info (rat male, Wistar, 260-290g; mice male, C57Bl6, 12-16 weeks old); ALZET brain infusion kit used; dose-response (pg 1718); post op. care (analgesics); behavioral testing (locomotor activity); neuropeptide K aka NPK;.

15120 Trachet, B., Fraga-Silva, R.A., Londono, F.J., Swillens, A., Stergiopulos, N., Segers, P., Trachet, B. Performance Comparison of Ultrasound-Based Methods to Assess Aortic Diameter and Stiffness in Normal and Aneurysmal Mice. PLoS One 2015; 10(--):U1707-U1726. >>> Angiotensin II; angiotensin (1-7); angiotensin (1-7), D-ala7-; Saline, sterile; SC; Mice; 2004; Controls received no mp; animal info (male, ApoE-/-, 20 weeks old); stress/adverse reaction: (see pg. 3); post op. care (buprenorphine 0.08 mg/kg SC); cardiovascular; peptides;.

15078 Sharma, R.K., Choudhary, R.C., Reddy, M.K., Ray, A., Ravi, K., Ravi, K. Role of posterior hypothalamus in hypobaric hypoxia induced pulmonary edema. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2015; 205(--):66-76. >>> Muscimol; SNAP; CSF, artificial; CSF/CNS (hypothalamus); Rat; 2001; Controls received mp w/ vehicle; animal info (male, Wistar, 280g); Multiple pumps per animal (2); post op. care (Gentamycin and diclofenac 2 mg/kg for 3 days; 1ml of sterile dextrose saline given IP; daily cleaning with betadine solution and nebasulf powder until wound healed); cardiovascular; antihypertensive;.

15057 Persson, P., Friederich-Persson, M., Fasching, A., Hansell, P., Inagi, R., Palm, F., Persson, P. Adenosine A(2)a receptor stimulation prevents proteinuria in diabetic rats by promoting an anti-inflammatory phenotype without affecting oxidative stress. Acta Physiologica 2015; 214(--):311-318. >>> CGS21680; DMSO; Ringer's solution; SC; Rat; 2004; Animal info (male, Sprague Dawley, 250-g, STZ); post op. care (carpofen 5 mg/kg SC); cardiovascular; immunology; antihypertensive; diabetes; CGS21680 is an A2aAR agonist;.

15947 Medzikovic, L., Schumacher, C. A., Verkerk, A. O., van Deel, E. D., Wolswinkel, R., van der Made, I., Bleeker, N., Cakici, D., van den Hoogenhof, M. M., Meggouh, F., Creemers, E. E., Remme, C. A., Baartscheer, A., de Winter, R. J., de Vries, C. J., Arkenbout, E. K., de Waard, V. Orphan nuclear receptor Nur77 affects cardiomyocyte calcium homeostasis and adverse cardiac remodelling. Sci Rep 2015; 5(--):15404. >>> Isoprotereneol; Saline; SC; Mice; animal info (WT or Nur77-KO); post op. care (Carpofen SC 4 mg/kg); Dose (60 mg/kg/day);.

14969 Hu, J.Z., Zeng, L., Huang, J.H., Wang, G., Lu, H.B., Lu, H.B. miR-126 promotes angiogenesis and attenuates inflammation after contusion spinal cord injury in rats. BRAIN RESEARCH 2015; 1608(--):191-202. >>> miR-126; Saline; CSF/CNS (intrathecal); Rat; 1003D; Controls received mp w/ control agomir; animal info (male, Sprague Dawley, 180-220g); spinal cord injury; post op. care (Ringer's solution administered IP 5 ml, penicillin G 40000 U IM QD for 3 days, bladders manually expressed BID); behavioral testing (locomotor testing, open field); immunology; pumps primed overnight at 37C.

14960 Ho, Y.H., Lin, Y.T., Wu, C.W.J., Chao, Y.M., Chang, A.Y.W., Chan, J.Y.H., Chan, J.Y.H. Peripheral inflammation increases seizure susceptibility via the induction of neuroinflammation and oxidative stress in the hippocampus. JOURNAL OF BIOMEDICAL SCIENCE 2015; 22(--):U1-U14. >>> Endotoxin, LPS; NS398; tempol; Saline; DMSO; IP; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 10 weeks old, 250-282g); ALZET brain infusion kit 2 used; 1% DMSO used; Multiple pumps per animal (2); post op. care (IM procaine penicillin 1000IU); immunology; used dental cement; NS398 is a COX-2 inhibitor and anti-inflammatory; tempol scavanges ROS;.

14409 Snijder, P.M., Frenay, A.R., de Boer, R.A., Pasch, A., Hillebrands, J.L., Leuvenink, H.G.D., van Goor, H., van Goor, H. Exogenous administration of thiosulfate, a donor of hydrogen sulfide, attenuates angiotensin II-induced hypertensive heart disease in rats. British Journal of Pharmacology 2015; 172(--):1494-1504. >>> Angiotensin II; SC; Rat; 2004; Controls received mp w/ NaCl; animal info (male, Sprague Dawley, 240-260g); post op. care (buprenorphine SC 50 ug/kg injection; 37C ventilated incubator); cardiovascular; peptides;.

14285 Lo, J.O., Schabel, M.C., Roberts, V.H.J., Morgan, T.K., Rasanen, J.P., Kroenke, C.D., Shoemaker, S.R., Spindel, E.R., Frias, A.E., Lo, J.O. Vitamin C supplementation ameliorates the adverse effects of nicotine on placental hemodynamics and histology in nonhuman primates. American Journal of Obstetrics and Gynecology 2015; 212(--):U271-U278. >>> Nicotine bitartrate; Water, bacteriostatic; SC; Monkey (macaque, pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Rhesus, pregnant, GD26); functionality of mp verified by blood levels; pumps replaced every 3 weeks; post op. care (cefazolin 150 mg BID); long-term study; teratology; dependence;.

14251 Kwiecien, J.M., Jarosz, B., Urdzikova, L.M., Rola, R., Dabrowski, W., Kwiecien, J.M. Subdural infusion of dexamethasone inhibits leukomyelitis after acute spinal cord injury in a rat model. FOLIA NEUROPATHOLOGICA 2015; 53(--):41-51. >>> Dexamethasone; PBS; CSF/CNS (intrathecal); Rat; 2ML2; Controls received mp w/ vehicle; animal info (Long Evans); spinal cord injury; post op. care (ketoprofen 0.3 ml of 10 mg/ml injection, saline administration 1-2 times daily); behavioral testing (Open field testing);"Subdural infusion of dexamethasone, a powerfully anti-inflammatory, stable, synthetic analogue of glucocorticoids, allowed for circumvention of the blood-brain barrier and apparently for achieving a sufficiently high concentration of this drug in the cerebrospinal fluid in proximity of the crush lesion to prevent severe, phagocyte-rich inflammation."pg 48; used rat IT catheter; dose: 2 mg/ml.

14204 Frias, B., Santos, J., Morgado, M., Sousa, M.M., Gray, S.M.Y., McCloskey, K.D., Allen, S., Cruz, F., Cruz, C.D., Cruz, C.D. The Role of Brain-Derived Neurotrophic Factor (BDNF) in the Development of Neurogenic Detrusor Overactivity (NDO). Journal of Neuroscience 2015; 35(--):2146-2160. >>> Brain-derived neurotropic factor; TrkB-Ig2; Tris buffer; glycerol; CSF/CNS (intrathecal); Rat; 2001; 2004; Controls received mp w/ saline; animal info (female, Wistar, 250-275g); 10% glycerol used; spinal cord injury; no stress (see pg. 2154); post op. care (ciprofloxacin 1 mg/kg IP injection; bladders manually emptied twice a day); used silicone catheter;.

14726 Eifert, A.W., Wilson, M.E., Vonnahme, K.A., Camacho, L.E., Borowicz, P.P., Redmer, D.A., Romero, S., Dorsam, S., Haring, J., Lemley, C.O., Lemley, C.O. Effect of melatonin or maternal nutrient restriction on vascularity and cell proliferation in the ovine placenta. ANIMAL REPRODUCTION SCIENCE 2015; 153(--):13-21. >>> Melatonin; luzindole; DMSO; water; Intrauterine; Sheep (ewe; pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Western white face, GD62); functionality of mp verified by serum levels; 45% DMSO used; good methods (pg 15); no stress (see pg. 15); post op. care (BID IM injection flunixin meglumine; QD IP injection Penicillin G Procaine); teratology; cardiovascular; used 20 cm of PE 60 tubing; pumps primed overnight 37C saline with catheters;.

14684 Case, A.J., Zimmerman, M.C., Zimmerman, M.C. Redox-Regulated Suppression of Splenic T-Lymphocyte Activation in a Model of Sympathoexcitation. Hypertension 2015; 65(--):916-+. >>> Norepinephrine; SC; Mice; 1002; Controls received mp w/ saline; animal info (male, C57BL6, 20-25g, 8-12 weeks old); functionality of mp verified by plasma levels; good methods (see supplement); post op. care (incision cleaned with iodine); cardiovascular; immunology; bp measured using radiotelemetry (DSI);.


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“In summary, our data demonstrate that the local microinfusion of endostatin is able to inhibit tumor growth and increase survival of animals with orthotopic malignant gliomas. Local continuous i.c. delivery is more effective than daily systemic delivery.” (p.1255) Schmidt et al., Clinical Cancer Research 2004;10:1255-1262.