References on the Use of ALZET® Osmotic Pumps Where Post Operative Care Was Mentioned

Q0734 Basselin,M., Ramadan,E., Chen,M., Rapoport,S.I. Anti-Inflammatory Effects of Chronic Aspirin on Brain Arachidonic Acid Metabolites. Neurochemical Research 2011; 36(-1-):139-145. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2002; 6 days; Controls received mp w/ vehicle; animal info (male, Fischer 344); post op. care (triple antibiotic ointment, sterile isotonic saline).

Q0804 Goergen,C.J., Azuma,J., Barr,K.N., Magdefessel,L., Kallop,D.Y., Gogineni,A., Grewall,A., Weimer,R.M., Connolly,A.J., Dalman,R.L., Taylor,C.A., Tsao,P.S., Greve,J.M. Influences of Aortic Motion and Curvature on Vessel Expansion in Murine Experimental Aneurysms. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2011; 31(-2-):270-U102. >>> Angiotensin II; PBS, Dulbecco's; SC; Mice; 2004; 28 days; Controls received mp w/ saline; animal info (apoE -/-, C57BL/6); peptides; post op. care (Buprenorphine); blood pressure was measured via tail cuff at days 0, 3, 7, 14, 21, and 28 (CODA System, Kent Scientific).

Q0702 Haque,M.Z., Majid,D.S.A. High Salt Intake Delayed Angiotensin II-Induced Hypertension in Mice With a Genetic Variant of NADPH Oxidase. American Journal of Hypertension 2011; 24(-1-):114-118. >>> Angiotensin II; Salin; acetic acid; SC; Mice; 1002; 2 weeks; Animal info ((8-9 wks old, 24-27 g, gp91PHOX KO, wt); peptides; post op. care (tetracycline in the water); SBP measured via tail-cuff plethysmographs (Visitech Systems); pumps incorrectly listed as 1002D.

Q0655 Polakof,S., Medale,F., Larroquet,L., Vachot,C., Corraze,G., Panserat,S. Insulin Stimulates Lipogenesis and Attenuates Beta-Oxidation in White Adipose Tissue of Fed Rainbow Trout. Lipids 2011; 46(-2-):189-199. >>> Insulin, bovine; IP; Fish (Rainbow trout); 5, 11 days; Controls received mp w/ saline; animal info (rainbow trout, 200 g); post op. care (antibiotic gel).

Q0705 Ren,P.G., Irani,A., Huang,Z.N., Ma,T., Biswal,S., Goodman,S.B. Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis. Clinical Orthopaedics and Related Research 2011; 469(-1-):113-122. >>> Polyethylene particles, UHMWPE; Saline; Bone (femur); Mice (nude); 2006; 3 weeks; Controls received mp w/ vehicle; animal info (12 wks old, male); post op. care (buprenorphine), pg 115; Fig 1A, diagram of pump and catheter placement; pump connected to titanium rod; bioluminescence; Ultra High Molecular Weight Polyethylene (UHMWPE).

Q0779 Sarkis,R., Saade,N., Atweh,S., Jabbur,S., Al-Amin,H. Chronic dizocilpine or apomorphine and development of neuropathy in two rat models I: Behavioral effects and role of nucleus accumbens. Experimental Neurology 2011; 228(-1-):19-29. >>> MK-801; apomorphine HCL hemihydrate; Saline; ascorbic acid; CSF/CNS (nucleus accumbens); Rat; 2002; ; Controls received mp w/ vehicle; animal info (adult, female, Sprague Dawley, 200-300 g); post op. care (dexamethasone injections to prevent brain edema); behavioral testing (mechanical allodynia, Paw withdrawal latency, cold allodynia, hotplate test, spontaneous motor activity); cannula placement verified by picomicrograph of brain section; CCI, chronic constriction injury; SNI, spared nerve injury.

Q0714 Yau,J.L.W., Noble,J., Seckl,J.R. 11beta-Hydroxysteroid Dehydrogenase Type 1 Deficiency Prevents Memory Deficits with Aging by Switching from Glucocorticoid Receptor to Mineralocorticoid Receptor-Mediated Cognitive Control. Journal of Neuroscience 2011; 31(-11-):4188-4193. >>> Mifepristone; spironolactone; CSF, artificial; propylene glycol; CSF/CNS; 1002; 10 days; Controls received mp w/ vehicle; animal info (HSD1-/-, C57BL/6J, 24 mo old); post op. care (buprenorphine); memory.

Q0863 Broadbent,N.J., Squire,L.R., Clark,R.E. Sustained Dorsal Hippocampal Activity is Not Obligatory for Either the Maintenance or Retrieval of Long-Term Spatial Memory. Hippocampus 2010; 20(-12-):1366-1375. >>> Lidocaine; CSF/CNS (hippocampus); Rat; 2ML2; 2ML1; 7 days; Controls received mp w/ aCSF; animal info (Long-Evans, naive, male, 300-350 g); good methods "Seven days after minipump implantation, rats were anesthetized with isoflurane gas, the tubing attached to the internal cannula sealed" pg 3; multiple pumps per animal (2); post op. care (Baytril antibiotic); multiple Plastics One cannulae used.

Q0108 Chakrabarty,S., Martin,J.H. Postnatal Development of a Segmental Switch Enables Corticospinal Tract Transmission to Spinal Forelimb Motor Circuits. Journal of Neuroscience 2010; 30(-6-):2277-2288. >>> Muscimol; Saline, sterile; CSF/CNS (motor cortex); Cat; 2002; 2 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit used; post op. care (cephalozine, buprenorphine); GABA-A agonist.

Q0479 Dang,M.H., Kato,H., Ueshiba,H., Omori-Miyake,M., Yamagoe,S., Ando,K., Imanishi,K., Arimura,Y., Haruta,I., Kotani,T., Ozaki,M., Suzuki,K., Uchiyama,T., Yagi,J. Possible role of LECT2 as an intrinsic regulatory factor in SEA-induced toxicity in D-galactosamine-sensitized mice. CLINICAL IMMUNOLOGY 2010; 137(-3-):311-321. >>> Antigen, staphylococcal enterotoxin A; SC; Mice; 2001; ; Animal info (B6, LECT2-/-, female, 8-12 wks old); post op. care (penicillin); immunology.

Q0399 Doura,M.B., Luu,T.V., Lee,N.H., Perry,D.C. PERSISTENT GENE EXPRESSION CHANGES IN VENTRAL TEGMENTAL AREA OF ADOLESCENT BUT NOT ADULT RATS IN RESPONSE TO CHRONIC NICOTINE. Neuroscience 2010; 170(-2-):503-513. >>> Nicotine hydrogen tartrate; Saline; SC; Rat; 2002; 14 days; Controls received mp w/vehicle; animal info (male, Sprague Dawley, 28-30 pn, 60-70 pn); post op. care (buprenorphine); wound clips used.

Q0074 Geremia,N.M., Pettersson,L.M.E., Hasmatali,J.C., Hryciw,T., Danielsen,N., Schreyer,D.J., Verge,V.M.K. Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons. Experimental Neurology 2010; 223(-1-):128-142. >>> Brain-derived neurotrophic factor, anti-, sheep; immunoglobulin G, sheep; CSF/CNS (intrathecal); Rat; 2001; 3 days; Controls received mp w/ control IgG; post op. care (buprenorphine); animal info (male, Wistar, adult, 300-500 g.); sciatic nerve transection.

Q0013 Goldshmit,Y., Munro,K., Leong,S.Y., Pebay,A., Turnley,A.M. LPA receptor expression in the central nervous system in health and following injury. Cell and Tissue Research 2010; 341(-1-):23-32. >>> ; CSF/CNS (cortex); Mice; 3 days; Cyanoacrylate adhesive; ALZET brain infusion kit used; cancer; animal info (C57BL/6, 3 months old); post op. care (metacam); no pump used.

Q0233 Gomez-Sanchez,E.P., Gomez-Sanchez,C.M., Plonczynski,M., Gomez-Sanchez,C.E. Aldosterone synthesis in the brain contributes to Dahl salt-sensitive rat hypertension. Experimental Physiology 2010; 95(-1-):120-130. >>> FAD286; Propylene glycol; saline; CSF/CNS; Rat; 2004; ; Controls received mp w/ vehicle; post op. care (buprenorphine); animal info (Sprague Dawley, Dahl, salt sensitive); cannula placement verified post mortem with a dye.

Q0073 Khoo,N.K.H., Rudolph,V., Cole,M.P., Golin-Bisello,F., Schopfer,F.J., Woodcock,S.R., Batthyany,C., Freeman,B.A. Activation of vascular endothelial nitric oxide synthase and heme oxygenase-1 expression by electrophilic nitro-fatty acids. Free Radical Biology and Medicine 2010; 48(-2-):230-239. >>> Oleic acid, nitro; oleic acid; SC; Mice; 7 days; Post op. care (Buprenex); animal info (male, C57BL/6J, 8-10 wks old); 9 mm wound clips used.

Q0175 Melendez,G.C., McLarty,J.L., Levick,S.P., Du,Y., Janicki,J.S., Brower,G.L. Interleukin 6 Mediates Myocardial Fibrosis, Concentric Hypertrophy, and Diastolic Dysfunction in Rats. Hypertension 2010; 56(-2-):225-231. >>> Interleukin-6; Albumin, rat; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; cardiovascular; no stress (pg 227); animal info (male, Sprague-Dawley, 250-300 g); post op. care (buprenorphine HCl).

Q0507 Mirrione,M.M., Konomos,D.K., Gravanis,I., Dewey,S.L., Aguzzi,A., Heppner,F.L., Tsirka,S.E. Microglial ablation and lipopolysaccharide preconditioning affects pilocarpine-induced seizures in mice. NEUROBIOLOGY OF DISEASE 2010; 39(-1-):85-97. >>> Ganciclovir; Saline; PBS; CSF/CNS (hippocampus); Mice; 1002; 7 days; Controls received mp w/ vehicle; post op. care (buprenorphine HCl); animal info (CD1 1b-, HSVTk +/-, wt, male, female, adult, 20-25 g); Plastics One guide cannula used.

Q0182 Polakof,S., Skiba-Cassy,S., Choubert,G., Panserat,S. Insulin-induced hypoglycaemia is co-ordinately regulated by liver and muscle during acute and chronic insulin stimulation in rainbow trout (Oncorhynchus mykiss). Journal of Experimental Biology 2010; 213(-9-):1443-1452. >>> Insulin, bovine; Saline; IP; Fish (trout); 1003D; 4 days; Controls received mp w/ vehicle; peptides; animal info (rainbow trout, 170 g); post op. care (antibiotic ointment); comparison of IP injections vs. mp; 17 degrees C; endocrinology.

Q0741 Senchenkova,E.Y., Russell,J., meida-Paula,L.D., Harding,J.W., Granger,D.N. Angiotensin II-Mediated Microvascular Thrombosis. Hypertension 2010; 56(-6-):1089-U174. >>> Angiotensin II; SC; Mice; 14 days; Animal info (AT1r -/-, wt); peptides; post op. care (topical antibotic, neosporin); blood pressure monitored via tail cuff (Hatteras Instruments).

Q0066 Shi,P., ez-Freire,C., Jun,J.Y., Qi,Y.F., Katovich,M.J., Li,Q.H., Sriramula,S., Francis,J., Sumners,C., Raizada,M.K. Brain Microglial Cytokines in Neurogenic Hypertension. Hypertension 2010; 56(-2-):297-303. >>> Minocycline; angiotensin II; SC; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ saline; ALZET brain infusion kit used; multiple pumps per animal (2); post op. care (buprenorphine); animal info (male, Sprague Dawley, 6 wks old).

Q0056 Strait,K.R., Orkin,J.L., Anderson,D.C., Muly,E.C. Chronic, Constant-Rate, Gastric Drug Infusion in Nontethered Rhesus Macaques (Macaca mulatta). Journal of the American Association for Laboratory Animal Science 2010; 49(-2-):207-214. >>> Antipsychotic; Stomach (gastric lumen); Monkey; 2ML2; 2ML4; 12 months; Controls received mp w/ saline; no stress (see pg. 210); long-term infusion; animal info (male, Rhesus Macaques, 8.12 - 12.18 kg, 5.25 - 7.5 years old); post op. care (buprenorphine, meloxicam); functionality of mp verified by residual volume, plasma drug concentrations; pumps replaced every 2-4 weeks; good methods (pg. 208-210); mp placed SC with a gastric catheter to the stomach; concurrent gastroscopy used confirm catheter tip placement; catheter patency during the year was confirmed by endoscopy; cranial PET scans every 2-4 weeks; agent and saline were alternated during the 52 weeks, as were drug concentrations; "All 5 macaques tolerated placement of the gastric catheter and pump changes well. The animals maintained good condition throughout the course of the yearlong study" (pg. 210); "This method offers an attractive alternative for use in studies that require continuous, constant rate, enteral drug delivery in untethered monkeys. (pg. 214).

P9895 Tran,L., Kompa,A.R., Kemp,W., Phrommintikul,A., Wang,B.H., Krum,H. Protective actions of estrogen on angiotensin. American Journal of Physiology-Heart and Circulatory Physiology 2010; 298(-2-):H608-H613. >>> Urotensin II, rat; Saline; IV (jugular); Rat; 2004; 28 days; Controls received mp w/vehicle, post op. care (buprenorphine); animal info (male, Sprague Dawley, 200-250 g).

Q0190 Troidl,C., Nef,H., Voss,S., Schilp,A., Kostin,S., Troidl,K., Szardien,S., Rolf,A., Schmitz-Rixen,T., Schaper,W., Hamm,C.W., Elsaesser,A., Moellmann,H. Calcium-dependent signalling is essential during collateral growth in the pig hind limb-ischemia model. Journal of Molecular and Cellular Cardiology 2010; 49(-1-):142-151. >>> Phorbol didecanoate, 4-alpha-; IA (femoral); Pig; 2ML1; 7 days; Controls received mp w/ saline or sham operation; cardiovascular; animal info (male, juvenile, crossbred, 38kg, femoral artery occlusion); post op. care (cefquinome, fentanyl); ischemia (hind limb); excellent color photograph of multiple mp placement (Fig. 1); multiple pumps per animal (2).

Q0060 Tschoep,J., Nogueiras,R., Haas-Lockie,S., Kasten,K.R., Castaneda,T.R., Huber,N., Guanciale,K., Perez-Tilve,D., Habegger,K., Ottaway,N., Woods,S.C., Oldfield,B., Clarke,I., Chua,S Jr, Farooqi,I.S., O'Rahilly,S., Caldwell,C.C., Tschoep,M.H. CNS Leptin Action Modulates Immune Response and Survival in Sepsis. Journal of Neuroscience 2010; 30(-17-):6036-6047. >>> Leptin; Saline, isotonic; SC; CSF/CNS; Mice; 1003D; 14 days; Controls received mp w/ vehicle; cyanoacrylate adhesive; ALZET brain infusion kit 3 used; animal info (male, ob/ob, wt, 8-10 wks old); post op. care (buprenorphine).

P9474 Amitai,N., Semenova,S., Markou,A. Clozapine attenuates disruptions in response inhibition and task efficiency induced by repeated phencyclidine administration in the intracranial self-stimulation procedure. European Journal of Pharmacology 2009; 602(-1-):78-84. >>> Clozapine; Hydrochloric acid; saline; SC; Rat; 2ML1; 14 days; Controls received mp w/ vehicle; pumps replaced after 7 days; post op. care (antibacterial ointment on incision area); animal info (Wistar rats, 300 g.); antipsychotic; subsequent pump implanted contralateral to the first minipump.

Q0762 Bruel-Jungerman,E., Veyrac,A., Dufour,F., Horwood,J., Laroche,S., Davis,S. Inhibition of PI3K-Akt Signaling Blocks Exercise-Mediated Enhancement of Adult Neurogenesis and Synaptic Plasticity in the Dentate Gyrus. PLoS One 2009; 4(-11-):U52-U61. >>> LY294002; DMSO; CSF, artificial; CSF/CNS; Rat; 2004; ; Controls received mp w/ vehicle; animal info (young, male, adult, Sprague-Dawley, 10-12 wks old); enzyme inhibitor (Phosphoinositide 3-kinase, PI3K); post op. care (topical antiseptic, exocptoplix); behavioral testing (running wheel); 30% DMSO used; aCSF recipe.

P9563 Chakrabarty,S., Shulman,B., Martin,J.H. Activity-Dependent Codevelopment of the Corticospinal System and Target Interneurons in the Cervical Spinal Cord. Journal of Neuroscience 2009; 29(-27-):8816-8827. >>> Muscimol; CSF/CNS (motor cortex); Cat (kitten); 2002; 2 weeks; Controls received mp w/saline; ALZET brain infusion kit used; post op. care (cefazolin, buprenorphine); tissue perfusion (motor cortex).

P9561 Chan,S.H.H., Wu,K.L.H., Chang,A.Y.W., Tai,M.H., Chan,J.Y.H. Oxidative Impairment of Mitochondrial Electron Transport Chain Complexes in Rostral Ventrolateral Medulla Contributes to Neurogenic Hypertension. Hypertension 2009; 53(-2-):217-U230. >>> Angiotensin II; phenylephrine; Saline; CSF, artificial; IP; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/vehicle; peptides; post op. care (Penicillin); animal info (SHR, 5-6 wks old, 11-12 wks old, Wky, 5-6 wks old).

Q0464 Chen,S.R., Samoriski,G., Pan,H.L. Antinociceptive effects of chronic administration of uncompetitive NMDA receptor antagonists in a rat model of diabetic neuropathic pain. Neuropharmacology 2009; 57(-2-):121-126. >>> Neramexane mesylate; memantine; gabapentin; Saline, sterile; water; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; animal info (male, 225-250 g, STZ induced diabetes); functionality of mp verified by plasma drug levels; post op. care (penicillin); wound clips used; neuropathic pain.

P9802 Fouad,K., Ghosh,M., Vavrek,R., Tse,A.D., Pearse,D.D. Dose and Chemical Modification Considerations for Continuous Cyclic AMP Analog Delivery to the Injured CNS. Journal of Neurotrauma 2009; 26(-5-):733-740. >>> Dibutyryl cAMP; cAMP, 8-bromo; Saline; CSF/CNS (intrathecal); CSF/CNS (motor cortex); Rat; 2002; 2, 4 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit used; post op. care (Buprenorphine); animal info (adult, female, Lewis, 180-200 g.); spinal cord injury.

Q0657 Fujita,K., Seike,T., Yutsudo,N., Ohno,M., Yamada,H., Yamaguchi,H., Sakumi,K., Yamakawa,Y., Kido,M.A., Takaki,A., Katafuchi,T., Tanaka,Y., Nakabeppu,Y., Noda,M. Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson's Disease. PLoS One 2009; 4(-9-):U121-U130. >>> MPTP; NaCl; SC; Mice; 2004; 28 days; Animal info (male, C57BL/6J, 8-12 wks old); comparison of acute injections vs SC mp; neurodegenerative (Parkinson's disease); post op. care (suture was disinfected with 70% ethanol everyday) pg e7246; behavioral testing (open field test); "Despite the lack of parkinsonian symptoms in the acute MPTP model in rodents, probably due to the low level of MAO-B in the rodent brain's capillaries, behavioral impairment could be observed in the chronic MPTP infusion model using an osmotic minipump to deliver the MPTP for long period. This could provide a better model for human PD." pg e7248.

Q0367 Fuller,D.D., Dougherty,B.J., Sandhu,M.S., Doperalski,N.J., Reynolds,C.R., Hayward,L.F. Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2009; 169(-3-):333-337. >>> Nicotine; SC; Rat (pregnant); 2ML4; 1, 2 weeks; Controls received mp w/ saline; animal info (Sprague-Dawley, female); functionality of mp verified by plasma nicotine levels; post op. care (buprenorphine, carprofen); wound clips used.

Q0287 Furuya,T., Hashimoto,M., Koda,M., Okawa,A., Murata,A., Takahashi,K., Yamashita,T., Yamazaki,M. Treatment of rat spinal cord injury with a Rho-kinase inhibitor and bone marrow stromal cell transplantation. Brain Research 2009; 1295(-;-):192-202. >>> Fasudil; Saline; CSF/CNS (intrathecal, subarachnoid space); Rat; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 8-10 wks old, 174-236 g); post op. care (Bacitramin); spinal cord injury.

Q0550 Ghia,J.E., Blennerhassett,P., Deng,Y.K., Verdu,E.F., Khan,W.I., Collins,S.M. Reactivation of Inflammatory Bowel Disease in a Mouse Model of Depression. Gastroenterology 2009; 136(-7-):2280-2288. >>> Reserpine; choline chloride; methyllycaconitine; Water; acetic acid; CSF/CNS; IP; Mice; 5, 10, 14 days; Controls received mp w/ vehicle; animal info (male, C57BL/6, 7-9 wks old, alpha 7nAchR -/-); post op. care (buprenorphine); Plastics One cannula used with PE60 tubing.

P9839 Gibrat,C., Saint-Pierre,M., Bousquet,M., Levesque,D., Rouillard,C., Cicchetti,F. Differences between subacute and chronic MPTP mice models: investigation of dopaminergic neuronal degeneration and alpha-synuclein inclusions. Journal of Neurochemistry 2009; 109(-5-):1469-1482. >>> MPTP; Saline; SC; IP; Mice; 2002; 2004; 14, 28 days; Controls received mp w/ vehicle; comparison of IP injections vs. SC mp vs. IP mp; post op. care (buprenorphine); animal info (male, young adult, C57BL/6, 9 wks old); neurodegenerative (Parkinson's Disease); behavioral testing (open field test); "14-day i.p.chronic MPTP treatment... replicates more accurately the pathological characteristics of human parkinsonism since this model offers the advantage of a much more progressive neuronal death of mesencephalic origin" pg 1480.

Q0271 Hilliard,L.M., Russo,L.M., Comper,W.D. Hypertension-Mediated Albuminuria Is Associated with Reduced Lysosomal Activity in the Kidney and the Heart. American Journal of Nephrology 2009; 29(-5-):454-464. >>> Albumin, c14; SC; Rat; 2001; 7 days; Animal info (Male SHR, WKY, 200-250 g); post op. care (0.002 w/v of thimerosal to prevent bacterial growth).

Q0386 Indrambarya,T., Boyd,J.H., Wang,Y.J., McConechy,M., Walley,K.R. Low-dose vasopressin infusion results in increased mortality and cardiac dysfunction following ischemia-reperfusion injury in mice. CRITICAL CARE 2009; 13(-3-):U361-U368. >>> Vasopressin, arginine; dobutamine; IP; Mice; 1003D; ; Controls received mp w/normal saline; animal info (10-14 wks old, male, C57B6); post op. care (buprenorphrine).

P9558 Kamendi,H.W., Cheng,Q., Dergacheva,O., Gorini,C., Jameson,H.S., Wang,X., McIntosh,J.M., Mendelowitz,D. Abolishment of Serotonergic Neurotransmission to Cardiac Vagal Neurons During and After Hypoxia and Hypercapnia With Prenatal Nicotine Exposure. Journal of Neurophysiology 2009; 101(-3-):1141-1150. >>> Nicotine; Rat; 28 days; Teratology; post op. care (buprenorphine) animal info (adult, female).

Q0046 Kemp,W., Kompa,A., Phrommintikul,A., Herath,C., Jia,Z.Y., Angus,P., McLean,C., Roberts,S., Krum,H. Urotensin II modulates hepatic fibrosis and portal hemodynamic alterations in rats. American Journal of Physiology-Gastrointestinal and Liver Physiology 2009; 297(-4-):G762-G767. >>> Urotensin II, rat, 125I; Saline; IV (jugular); Rat; 2004; 28 days; Controls received mp w/ vehicle; peptides; cardiovascular; no stress (see pg. G763); animal info (male, Sprague-Dawley, 200-250 g); post op. care (buprenorphine); dose-response Fig. 1; hepatology.

P9353 Matsushita,H., Ishihara,A., Mashiko,S., Tanaka,T., Kanno,T., Iwaasa,H., Ohta,H., Kanatani,A. Chronic Intracerebroventricular Infusion of Nociceptin/Orphanin FQ Produces Body Weight Gain by Affecting Both Feeding and Energy Metabolism in Mice. Endocrinology 2009; 150(-6-):2668-2673. >>> Nociceptin/OrphaninFQ; Saline; BSA; CSF/CNS; Mice; 2002; 2004; 12 days; Controls received mp w/ vehicle; saline pumps replaced after 2 week recovery period with N/OFQ filled pumps; ALZET brain infusion kit used; post op. care (Cefamezin alpha); animal info (male, C57BL/6, 17 or 28 wks old); N/OFQ is an endogenous ligand for opioid receptor-like 1 (ORL1).

P9355 Miller,A.M., Piazza,A., Martin,D.S.D., Walsh,M., Mandel,A., Bolton,A.E., Lynch,M.A. The deficit in long-term potentiation induced by chronic administration of amyloid-beta is attenuated by treatment of rats with a novel phospholipid-based drug formulation, VP025. Experimental Gerontology 2009; 44(-4-):300-304. >>> Amyloid protein, beta (1-40); amyloid protein, beta (1-42); CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ amyloid protein, beta (40-1); ALZET brain infusion kit 2 used; post op. care (Rimadil); animal info (male, Wistar, 3-4 months old).

P9551 Motyl,K.J., McCabe,L.R. Leptin Treatment Prevents Type I Diabetic Marrow Adiposity But Not Bone Loss in Mice. Journal of Cellular Physiology 2009; 218(-2-):376-384. >>> Leptin; Saline, sterile; SC; Mice; 2004; ; Controls received mp w/vehicle; post op. care (Carprofen); animal info (14 wks old, BALB/c).

Q0333 Nguyen,Y., Couloigner,V., Rudic,M., Grayeli,A.B., Ferrary,E., Sterkers,O. An animal model of cochlear implantation with an intracochlear fluid delivery system. Acta Oto-laryngologica 2009; 129(-11-):1153-1159. >>> Saline; Ear (cochlea); Guinea pig; 1007D; ; Controls received pump without electrode implant; animal info (albino, male, 3 to 7 months old, 290-1030 g.); good methods pg 1154; post op. care (enrofloxacine); "the pump was fixed subcutaneously between the scapulae using a vicryl 3/0 suture (Ethicon)" pg 1154; image of pump-electrode deive, Fig. 1; tissue perfusion (cochlea).

P9652 Nogueiras,R., Perez-Tilve,D., Veyrat-Durebex,C., Morgan,D.A., Varela,L., Haynes,W.G., Patterson,J.T., Disse,E., Pfluger,P.T., Lopez,M., Woods,S.C., DiMarchi,R., Dieguez,C., Rahmouni,K., Rohner-Jeanrenaud,F., Tschoep,M.H. Direct Control of Peripheral Lipid Deposition by CNS GLP-1 Receptor Signaling Is Mediated by the Sympathetic Nervous System and Blunted in Diet-Induced Obesity. Journal of Neuroscience 2009; 29(-18-):5916-5925. >>> Glucagon-like peptide-1 (7-36); exendin (9-39); CSF/CNS; Mice; 1003D; 1007D; 7 days; 48 hours; Controls received mp w/Saline; ALZET brain infusion kit 3 used; post op. care (buprenorphine); animal info (8 wks old, wt, eTKO).

P9615 Novak,K.R., Nardelli,P., Cope,T.C., Filatov,G., Glass,J.D., Khan,J., Rich,M.M. Inactivation of sodium channels underlies reversible neuropathy during critical illness in rats. Journal of Clinical Investigation 2009; 119(-5-):1150-1158. >>> Oxymorphone; IM (abdominal); Rat; ; Post op. care (buprenorphine); animal info (female, Wistar, 250-300g).

P9844 Orita,H., Shimogori,H., Yamashita,H. Unilateral intra-perilymphatic infusion of substance P enhances ipsilateral vestibulo-ocular reflex gains in the sinusoidal rotation test. Neuroscience Letters 2009; 449(-3-):207-210. >>> Substance P; neurokinin-1 receptor antagonist; Ear (round window); Guinea-pig; 2002; ; Post op. care (piperacillin sodium); animal info (Hartley); pump was connected to a PE catheter filled with artificial perilymph for a 12-hour delayed infusion; tissue perfusion (round window).

Q0375 Pedersen,C.S., Goetghebeur,P., Dias,R. Chronic infusion of PCP via osmotic mini-pumps: A new rodent model of cognitive deficit in schizophrenia characterized by impaired attentional set-shifting (ID/ED) performance. Journal of Neuroscience Methods 2009; 185(-1-):66-69. >>> Phencyclidine; SC; Rat; 14 days; Controls received mp w/saline; animal info (Lister hooded, male); post op. care (Baitril, Rimadyl); "Using PCP mini-pump infusion instead of the well described intraperitoneal dosing bears the advantage of reducing the animal's stress levels, bypasses the risk of potential mis-dosing that could arise from multiple dosing events and consequently may reduce the number of animals needed." pg 69.

P9810 Romo,L.B., Santa-Cruz,L.D., Zepeda,A., Tapia,R. Chronic elevation of extracellular glutamate due to transport blockade is innocuous for spinal motoneurons in vivo. NEUROCHEMISTRY INTERNATIONAL 2009; 54(-3-4-):186-191. >>> PDC; TBOA; CSF/CNS (intrathecal); Rat; 2004; ; Controls received mp w/ Ringer Krebs medium; good methods pg 187; post op. care (penicillin, IM); animal info (adult, male, Wistar, 290-310 g.); PDC also known as L-2,4-trans-pyrrolidinedicarboxylate is a non-selective glutamate transporter blocker; TBOA.

P9519 Sarahrudi,K., Mousavi,M., Grossschmidt,K., Sela,N., Koenig,F., Vecsei,V., Aharinejad,S. The Impact of Colony-Stimulating Factor-1 on Fracture Healing: An Experimental Study. Journal of Orthopaedic Research 2009; 27(-1-):36-41. >>> Colony-stimulating factor-1; SC; Rabbit; 2ML2; 2 weeks; Controls were surgically stabilized or received no treatment; post op. care (buprenorphine); animal info (New Zealand, 4-4.5 kg).

Q0525 Schaub,C.E., Wood,C.E. Blockade of Estrogen Action Upregulates Estrogen Receptor-alpha mRNA in the Fetal Brain. Neonatology 2009; 96(-2-):115-119. >>> ICI 182,780; DMSO; water; CSF/CNS; Sheep (ewe); 2ML4; 6-14 days; Twin fetus control received mp w/ vehicle; animal info (fetus, twins); post op. care (ampicillin); 50% DMSO used; ICV catheter placement verified post mortem.

Q0282 Schmidt,C., Bezuidenhout,D., Beck,M., Van der Merwe,E., Zilla,P., Davies,N. Rapid three-dimensional quantification of VEGF-induced scaffold neovascularisation by microcomputed tomography. Biomaterials 2009; 30(-30-):5959-5968. >>> Vascular endothelial growth factor (165); SC; Rat; 1002; 10 days; Controls received mp w/ PBS; animal info (male, Wistar, 234-254 g); functionality of mp verified by residual volume; post op. care (buprenorphine); pump connected to a porous polyurethane construct, schematic on Figure 1A;.

Q0576 Serbedzija,P., Madl,J.E., Ishii,D.N. Insulin and IGF-I prevent brain atrophy and DNA loss in diabetes. Brain Research 2009; 1303(-;-):179-194. >>> Insulin; insulin-like growth factor-I; CSF/CNS; Rat; 12 weeks; Controls received mp w/ aCSF; animal info (Wistar, 275-300 g adult, 9 wks old, male, STZ-induced diabetes); long-term study; pumps replaced at two week intervals; ALZET brain infusion kit used; post op. care (Buprenorphine); cannula placement verified by injecting 20 ul dye through the catheter at time of assay.

P9689 Srivareerat,M., Tran,T.T., Alzoubi,K.H., Alkadhi,K.A. Chronic Psychosocial Stress Exacerbates Impairment of Cognition and Long-Term Potentiation in beta-Amyloid Rat Model of Alzheimer's Disease. Biological Psychiatry 2009; 65(-11-):918-926. >>> Amyloid protein, beta (1-40); amyloid protein, beta (1-42); Water, distilled; acetonitrile; trifluoroacetate; CSF/CNS; Rat; 2 weeks; Controls received mp w/ inactive reverse peptide, amyloid protein beta (40-1); post op. care (antibiotic ointment); animal info (male, Wistar, 200-225 g.); neurodegenerative (Alzheimer's Disease).

Q0449 Stockwell,K.A., Scheller,D., Rose,S., Jackson,M.J., Tayarani-Binazir,K., Iravani,M.M., Smith,L.A., Olanow,C.W., Jenner,P. Continuous administration of rotigotine to MPTP-treated common marmosets enhances anti-parkinsonian activity and reduces dyskinesia induction. Experimental Neurology 2009; 219(-2-):533-542. >>> Rotigotine; Saline, sterile; SC; Monkey (marmoset); 2004; 58 days; Controls received mp w/ vehicle; animal info (adult common, male, female, 354 g); comparison of sc injections vs. mp; neurodegenerative (Parkinson's disease); post op. care (Rimadyl, Synulox); "pumps were removed... and replaced with new minipumps implanted into the opposite flank." pg 534; "These results demonstrate that the anti-parkinsonian benefits associated with a continuous infusion of rotigotine were more sustained compared to pulsatile rotigotine or L-DOPA treatment..." pg 541.

Q0378 Troidl,C., Troidl,K., Schierling,W., Cai,W.J., Nef,H., Moellmann,H., Kostin,S., Schimanski,S., Hammer,L., Elsaesser,A., Schmitz-Rixen,T., Schaper,W. Trpv4 induces collateral vessel growth during regeneration of the arterial circulation. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2009; 13(-8B-):2613-2621. >>> PDD, 4 alpha; ruthenium red; Ethanol; NaCl; IA (femoral); Rabbit; rat; 7 days; Controls received mp w/vehicle or sham operated; animal info (New Zealand White, 3.0 kg, Sprague Dawley, 275 g); post op. care (buprenorphine); 4-alpha PDD also known as Phorbol-12,13-didecanoate; Fig 1, schematic of femoral artery ligature with ALZET pump.

P9827 Uebele,V.N., Nuss,C.E., Santarelli,V.R., Garson,S.L., Barrow,J.C., Stauffer,S.R., Koblan,K.S., Renger,J.J., Aton,S., Seibt,J., Dumoulin,M., Jha,S.K., Coleman,T., Frank,M.G. T-type calcium channels regulate cortical plasticity in-vivo NR-D-08-7049. NeuroReport 2009; 20(-3-):257-262. >>> TTA-I1; DMSO; CSF, artificial; CSF/CNS (visual cotex); Cat; 2ML2; ; Controls received mp w/ vehicle; ALZET brain infusion kit used; post op. care (antibiotics, analgesics); animal info (36 days); 50% DMSO used; TTA-I1 is a T-typed calcium channel antagonist.

P9573 Wang,X., LeVatte,T.L., Archibalda,M.L., Chauhan,B.C. Increase in endothelin B receptor expression in optic nerve astrocytes in endothelin-1 induced chronic experimental optic neuropathy. Experimental Eye Research 2009; 88(-3-):378-385. >>> Endothelin-1; CSF/CNS (optic nerve); Rat; 2004; 28 days; Post op. care (buprenorphine); animal info (adult, male, Brown Norway, 250-300g); fellow eye served as untreated control; silastic tubing used; brain tissue distribution.

P9462 White,C.L., Whittington,A., Barnes,M.J., Wang,Z., Bray,G.A., Morrison,C.D. HF diets increase hypothalamic PTP1B and induce leptin resistance through both leptin-dependent and -independent mechanisms. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2009; 296(-2-):E291-E299. >>> Leptin; Saline; SC; CSF/CNS; Rat; mice; 10, 14 days; Controls received mp w/ vehicle; comparison of IP, ICV injections vs. mp; peptides; post op. care (analgesics); animal info (male, Long Evans, 10 wks old; C57BL/6 wt, ob/ob, 8 wks old).

P9464 Wood,C.E., Fraites,M.P., Keller-Wood,M. Blockade of PGHS-2 inhibits the hypothalamus-pituitary-adrenal axis response to cerebral hypoperfusion in the sheep fetus. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 296(-6-):R1813-R1819. >>> Nimesulide; DMSO; water; CSF/CNS; Sheep (fetus); 2ML2; 10 days; Controls received mp w/ vehicle; enzyme inhibitor (prostaglandin endoperoxide synthase-2); teratology; post op. care (ampicillin); animal info (fetal sheep, GD 126-136); 50% DMSO used; "chronic infusion of the drug intracerebroventricularly, for days, has a dramatic effect of reducing fetal HPA axis activity." (p. R1818).

P9880 Wu,K.L.H., Hsu,C., Chan,J.Y.H. Nitric Oxide and Superoxide Anion Differentially Activate Poly(ADP-ribose) Polymerase-1 and Bax to Induce Nuclear Translocation of Apoptosis-Inducing Factor and Mitochondrial Release of Cytochrome c after Spinal Cord Injury. Journal of Neurotrauma 2009; 26(-7-):965-977. >>> Methylisothiourea, S-; PTIO, carboxy-; Coenzyme Q10; tempol; FeTMPyP; furosemide; PJ-34; CSF, artificial; sesame oil; CSF/CNS (spinal cord); Rat; 1003D; 3 days; Controls received mp w/ vehicle; multiple pumps per animal (2); post op. care (procraine penicillin); adult, male, specific pathogen free, 200-250 g.); spinal cord injury; PE-10 catheter used; PTIO is a NO trapping agent; enzyme inhibitor PJ-34 is poly(ADP-ribose) polymerase PARP inhibitor.

P9739 Zai,L.L., Ferrari,C., Subbaiah,S., Havton,L.A., Coppola,G., Strittmatter,S., Irwin,N., Geschwind,D., Benowitz,L.I. Inosine Alters Gene Expression and Axonal Projections in Neurons Contralateral to a Cortical Infarct and Improves Skilled Use of the Impaired Limb. Journal of Neuroscience 2009; 29(-25-):8187-8197. >>> Inosine; Saline; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/vehicle; post op. care (buprenex); animal info (adult, Sprague Dawley, 275-300 g); behavioral testing (forelimb reaching).

P9681 Ahmad,M., White,R., Tan,J.H., Huang,B.S., Leenen,F.H.H. Angiotensin-converting enzyme inhibitors, inhibition of brain and peripheral angiotensin-converting enzymes, and left ventricular dysfunction in rats after myocardial infarction. Journal of Cardiovascular Pharmacology 2008; 51(-6-):565-572. >>> Lisinopril; transdolapril; CSF, artificial; CSF/CNS; Rat; 2002; 4 weeks; Controls received mp w/ vehicle; pumps replaced after 2 weeks; enzyme inhibitor (ACE); post op. care (acetaminophen, buprenoriphine); animal info (male, Wistar, 200-250 g.); silastic tubing used.

P9290 Alahmed,S., Herbert,J. STRAIN DIFFERENCES IN PROLIFERATION OF PROGENITOR CELLS IN THE DENTATE GYRUS OF THE ADULT RAT AND THE RESPONSE TO FLUOXETINE ARE DEPENDENT ON CORTICOSTERONE. Neuroscience 2008; 157(-3-):677-682. >>> Fluoxetine; Propylene glycol; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; comparison of daily IP injections vs. mp; post op. care (Buprenorphine); animal info (male, Sprague Dawley, Lister-hooded, 200-250 g.); "The activity of progenitor cells in either strain did not respond to daily i.p. injections of fluoexetine (10 mg/kg) for 14 days, but an equivalent dose administered by osmotic minipump stimulated proliferation in both by a similar proportional amount." (p. 677).

P9080 Aragon,A.C., Goens,M.B., Carbett,E., Walker,M.K. Perinatal 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure sensitizes offspring to angiotensin II-induced hypertension. Cardiovascular Toxicology 2008; 8(-3-):145-154. >>> Angiotensin II; SC; Mice; 1002; 14 days; Controls received mp w/ NaCl; peptides; post op. care (buprenorphine); animal info (male, female, C57BL/6, 6-8 wks old).

P8929 Badlani,N., Inoue,A., Healey,R., Coutts,R., Amiel,D. The protective effect of OP-1 on articular cartilage in the development of osteoarthritis. OSTEOARTHRITIS AND CARTILAGE 2008; 16(-5-):600-606. >>> Osteogenic protein-1; Lactose; Knee (articular cavity); Rabbit; 2004; 6 weeks; Controls received mp w/ vehicle; functionality of mp verified by separate in vivo test; peptides; post op. care ("appropriate"); animal info (New Zealand, White, 1 yr old); also known as BMP-7 or bone morphogenic protein-7; catheter was secured with polyethylene stents; "The efficacy of the (ALZET Pump) was tested in vivo before beginning the study with good results"; "constant infusion of OP-1 may be the most ideal form of delivery"; long-term study; good methods.

P9289 Bechtholt,A.J., Valentino,R.J., Lucki,I. Overlapping and distinct brain regions associated with the anxiolytic effects of chlordiazepoxide and chronic fluoxetine. Neuropsychopharmacology 2008; 33(-9-):2117-2130. >>> Fluoxetine HCl; Ethanol; water, sterile distilled, deionized; SC; Rat; 3, 28 days; Controls received mp w/ vehicle; dose-response (fig. 3); no stress (see pg. 2121); post op. care (Betadine); animal info (male, Sprague Dawley, 225-250 g.); behavioral testing (novelty-induced hypophagia test (NIH)); 50% EtOH used; "Continued administration (of fluoxetine) for a chronic period may be required for elicitation of the associated behavioral effects." (p.2127).

P9126 Bondi,C.O., Rodriguez,G., Gould,G.G., Frazer,A., Morilak,D.A. Chronic unpredictable stress induces a cognitive deficit and anxiety-like behavior in rats that is prevented by chronic antidepressant drug treatment. Neuropsychopharmacology 2008; 33(-2-):320-331. >>> Desipramine; escitalopram; Ethanol; saline; IP; Rat; 2ML4; ; Post op. care (penicillin); animal info (Sprague Dawley, 200-250 g.); 10% ethanol used; behavioral testing (chronic unpredictable stress).

P9260 Bordia,T., Campos,C., Huang,L.P., Quik,M. Continuous and intermittent nicotine treatment reduces L-3,4-dihydroxyphenylalanine (L-DOPA)-induced dyskinesias in a rat model of Parkinson's disease. Journal of Pharmacology and Experimental Therapeutics 2008; 327(-1-):239-247. >>> Nicotine, free base; Water, sterilized; SC; Rat; 2004; 2006; 8-10 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma continine levels; comparison of PO vs. mp; long-term study pumps replaced after 4 weeks; post op. care (buprenorphine); animal info (male, Sprague Dawley, 6-H DA lesion, 250 g.); neurodegenerative (Parkinson's disease); behavioral testing (forelimb asymmetry test, axial dystonia, orolingual movement, rotational behavior).

P9392 Bosch,O.J., Neumann,I.D. Brain vasopressin is an important regulator of maternal behavior independent of dams' trait anxiety. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-44-):17139-17144. >>> Vasopressin, arginine; oxytocin; oligonucleotide, antisense; oligonucleotide, missense; Ringer's solution; CSF/CNS; CSF/CNS (medial preoptic area); Rat (pregnant); 1007D; 5-7 days; Controls received mp w/ vehicle; comparison of ICV injections vs. mp; antisense (AVP-R); peptides; multiple pumps per animal (2); post op. care (Enrofloxacin, Baytril); animal info (female, Wistar, HAB, LAB, 14 wks old); behavioral testing (maternal care, pup retrieval test, elevated plus maze); cannula placement and infusion sites verified with blue ink.

P8893 Brenneman,M.M., Wagner,S.J., Cheatwood,J.L., Heldt,S.A., Corwin,J.V., Reep,R.L., Kartje,G.L., Mir,A.K., Schwab,M.E. Nogo-A inhibition induces recovery from neglect in rats. Behavioural Brain Research 2008; 187(-2-):262-272. >>> Antibody, anti-Nogo-A monoclonal 7B12; antibody, anti-Nogo-A monoclonal 11C7; PBS; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ control IgG antibody; no stress (see pg. 264); good methods (pg. 264); post op. care ("clean, warm environment"); pumps removed after 21 days.

P8816 Depino,A.M., Tsetsenis,T., Gross,C. GABA homeostasis contributes to the developmental programming of anxiety-related behavior. Brain Research 2008; 1210(--):189-199. >>> Diazepam; DMSO; propylene glycol; SC; Mice; mice (neonate); 1002; 2002; 14 days; Controls received mp w/ vehicle; dose-response (Fig. 1); no stress (see pg. 193); post op. care (flumazenil); animal info (C57BL/6 x 129/SvJ, male, 14 days old, 60 days old); 50% DMSO used; behavioral testing (maze); wound clips used; 50% propylene glycol used.

P9200 Doerr,M.D., Goravanahally,M.P., Rhinehart,J.D., Inskeep,E.K., Flores,J.A. Effects of endothelin receptor type-A and type-B antagonists on prostaglandin F2alpha-induced luteolysis of the sheep corpus luteum. Biology of Reproduction 2008; 78(-4-):688-696. >>> BQ123; BQ610; BQ788; Methanol; saline; Intraovarian (corpus luteum); Sheep; 2002; 14 days; Controls received mp w/ vehicle; tissue perfusion (corpus luteum); good methods (pg. 689); post op. care (penicillin); animal info (female); pumps were primed overnight prior to surgery; vinyl catheter tubing (0007760) used; catheter stabilized to connective tissue capsule and ovarian tunica albuginea using nylon monofilament; all compounds are EDNRA type-A endothelin antagonists.

P8834 Festuccia,W.T., Oztezcan,S., Laplante,M., Berthiaume,M., Michel,C., Dohgu,S., Denis,R.G., Brito,M.N., Brito,N.A., Miller,D.S., Banks,W.A., Bartness,T.J., Richard,D., Deshaies,Y. Peroxisome proliferator-activated receptor-gamma-mediated positive energy balance in the rat is associated with reduced sympathetic drive to adipose tissues and thyroid status. Endocrinology 2008; 149(-5-):2121-2130. >>> Rosiglitazone maleate; Saline, sterile; DMSO; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; dose-response (Table 3); comparison of oral vs. mp; post op. care (ketoprophene); animal info (male, Sprague Dawley, 175-200g.); also known as BRL49653; 12.5% DMSO used; cannula position confirmed post mortem; endocrinology; PPAR-gamma agonist.

P9237 Galvao,R.P., Garcia-Verdugo,J.M., varez-Buylla,A. Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats. Journal of Neuroscience 2008; 28(-50-):13368-13383. >>> Ara-C; brain-derived neurotrophic factor; Saline; BSA; CSF/CNS; Rat; mice; 1007D; 2002; 6, 14 days; Controls received mp w/ vehicle; functionality of mp verified by weight loss due to BDNF's appetite suppression (p. 13377), residual volume; stability verified by testing activity of residual BDNF volume after 14 days/37 degrees Celsius; brain tissue distribution; peptides; post op. care (Buprenorphine); animal info (male, Sprague Dawley, 250 g., male CD-1 3 mo old); mp/cannula placement visually confirmed after brain sectioning.

P9189 Gersting,J., Schaub,C.E., Keller-Wood,M., Wood,C.E. Inhibition of brain prostaglandin endoperoxide synthase-2 prevents the preparturient increase in fetal adrenocorticotropin secretion in the sheep fetus. Endocrinology 2008; 149(-8-):4128-4136. >>> Nimesulide; DMSO; water; CSF/CNS; Sheep; 2ML2; 2ML4; ; Controls received mp w/ vehicle; post op. care (ampicillin); animal info (ewe, fetus, 119-125 d gestation); nimesulide is a COX-2 selective NSAID; incisions were sutured; 50% DMSO used.

P9284 Griesbach,G.S., Hovda,D.A., Gomez-Pinilla,F., Sutton,R.L. Voluntary exercise or amphetamine treatment, but not the combination, increases hippocampal brain-derived neurotrophic factor and synapsin I following cortical contusion injury in rats. Neuroscience 2008; 154(-2-):530-540. >>> Amphetamine sulfate; Saline, sterile; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; no stress (see pg. 535); post op. care (Bupivacaine, antibiotic ointment); ischemia (cerebral); animal info (male, Sprague Dawley, 285 g., CCI injury); behavioral testing (voluntary wheel exercise); "This drug administration method was chosen since our prior studies of RW exposure after TBI have left animals undistrurbed for the 1 week exercise period." (p. 530).

P9042 Gutman,R., Hacmon-Keren,R., Choshniak,I., Kronfeld-Schor,N. Effect of food availability and leptin on the physiology and hypothalamic gene expression of the golden spiny mouse: a desert rodent that does not hoard food. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 295(-6-):R2015-R2023. >>> Leptin; SC; Mice; 2002; 23 days; Controls received mp w/ PBS; pumps replaced after 14 days; peptides; post op. care (Baytril); animal info (golden, spiny, male).

P8761 Hildreth,C.M., Padley,J.R., Pilowsky,P.M., Goodchild,A.K. Impaired serotonergic regulation of heart rate may underlie reduced baroreflex sensitivity in an animal model of depression. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-1-):H474-H480. >>> Metoprolol; atropine methylnitrate; SC; Rat; 3 days; Cardiovascular; post op. care (carprofen, cephazolan); animal info (male, Sprague Dawley Flinders Resistive Line, Flinders Sensitive Line, 10-14 wks old).

P9330 Hobbs,C.E., Murphy,M.P., Smith,R.A.J., Oorschot,D.E. Neonatal rat hypoxia-ischemia: Effect of the anti-oxidant mitoquinol, and S-PBN. PEDIATRICS INTERNATIONAL 2008; 50(-4-):481-488. >>> Mitoquinone; NaCl; ethanol; CSF/CNS (striatum); Rat (pregnant); 1003D; 3 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; post op. care (naloxone); animal info (albino, Sprague Dawley); 10% ethanol used; pumps were primed in 37 degrees Celsius Saline.

P9177 Holmberg,E., Zhang,S.X., Sarmiere,P.D., Kluge,B.R., White,J.T., Doolen,S. Statins decrease chondroitin sulfate proteoglycan expression and acute astrocyte activation in central nervous system injury. Experimental Neurology 2008; 214(-1-):78-86. >>> Simvastatin; CSF/CNS; Rat; 2004; 4 weeks; Comparison of PO gavage vs. mp; ALZET brain infusion kit used; post op. care (buprenorphine); animal info (female, Long Evans, adult, 200-22 g.); cannula placement confirmed by preparing cryosections of the brain at the lateral ventricle and verifying the presence of a cavity created by the cannula; spinal cord injury.

P9079 Hurtado,A., Podinin,H., Oudega,M., Grimpe,B. Deoxyribozyme-mediated knockdown of xylosyltransferase-1 mRNA promotes axon growth in the adult rat spinal cord. Brain 2008; 131(-;-):2596-2605. >>> DNAXTas; CSF/CNS (spinal cord, intrathecal); Rat; 1007D; 14 days; Controls received mp w/ control deoxyribozyme or mp w/ saline; pumps replaced after 7 days; post op. care (gentamicin); animal info (female, Sprague Dawley, 200-225 g.); control also known as DNAXTmb; schematic w/ pump on fig. 1; custom cannula used.

P9273 Ito,M., Gomori,A., Suzuki,J., Tsujioka,S., Sasaki,M., Matsuda,M., Bednarek,M.A., Ito,M., Ishihara,A., Iwaasa,H., MacNeil,D.J., Kanatani,A. Antagonism of central melanin-concentrating hormone 1 receptor alleviates steatohepatitis in mice. Journal of Endocrinology 2008; 198(-2-):309-315. >>> Melanin-concentrating hormone 1, receptor antagonist; Water, distilled; propylene glycol; CSF/CNS; Mice; 2004; 4, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 2 or 4 weeks; no stress (see pg. 311, 314); ALZET brain infusion kit used; peptides; post op. care (Cefamezin); animal info (male, C57BL/6, 10 wks old, 16 wks old, 1 year old); animals received mp w/vehicle for 2 or 4 weeks, then mp with agent or vehicle for 2 or 4 more weeks; dental cement used; cannula placement confirmed at end of experiment with Evans blue dye.

P9485 Johnson,P.M., Hollander,J.A., Kenny,P.J. Decreased brain reward function during nicotine withdrawal in C57BL6 mice: Evidence from intracranial self-stimulation (ICSS) studies. Pharmacology Biochemistry and Behavior 2008; 90(-3-):409-415. >>> Nicotine hydrogen tartrate; Saline, physiological; SC; Mice; 2004; 13 days; Controls received mp w/ vehicle; comparison of SC injections vs. mp; dependence; post op. care (bacitracin ointment); animal info (male, C57BL/6, 6 wks old); behavioral testing (intracranial self stimulation threshold, brain reward function).

P8764 Klieverik,L.P., Sauerwein,H.P., Ackermans,M.T., Boelen,A., Kalsbeek,A., Fliers,E. Effects of thyrotoxicosis and selective hepatic autonomic denervation on hepatic glucose metabolism in rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-3-):E513-E520. >>> Thyroxine, L-; Propylene glycol; NaOH; SC; Rat; 2ML2; 10 days; Functionality of mp verified by T4 plasma concentrations; dose-response (Fig. 1); no stress (see pg. E515); post op. care (Temgesic); animal info (male, Wistar, 325-375g.); hepatic sympathetic or parasympathetic denervation.

P9425 Leranth,C., Hajszan,T., Szigeti-Buck,K., Bober,J., MacLusky,N.J. Bisphenol A prevents the synaptogenic response to estradiol in hippocampus and prefrontal cortex of ovariectomized nonhuman primates. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-37-):14187-14191. >>> Bisphenol A; Propylene glycol; SC; Monkey; 2ML4; 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; replacement therapy (ovariectomy); post op. care (buprenorphine, carprofen); animal info (female, African green, 4-5 kg.).

P9258 Leskinen,H., Rauma-Pinola,T., Szokodi,I., Kerkelae,R., Pikkarainen,S., Uusimaa,P., Hautala,T., Vuolteenaho,O., Ruskoaho,H. Adaptive or maladaptive response to adenoviral adrenomedullin gene transfer is context-dependent in the heart. JOURNAL OF GENE MEDICINE 2008; 10(-8-):867-877. >>> Angiotensin II; SC; Rat; 2001; 2002; 3-14 days; Controls received mp no agent infusion; cardiovascular; peptides; post op. care (buprenorphine hydrochloride) ischemia (cardiac); animal info (male, Sprague Dawley, MI).

P8909 Liao,Y.J., Safa,P., Chen,Y.R., Sobel,R.A., Boyden,E.S., Tsien,R.W. Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-7-):2705-2710. >>> Immunoglobulin G, rabbit; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice; 1003D; 3 days; Functionality of mp verified w/ Alexa 488-labeled goat anti-rabbit IgG staining of coronal sections through the cerebellum); ALZET brain infusion kit 3 used; post op. care (buprenorphine); animal info (C57BL/6, > 20 g.); neurodegenerative (cerebellar ataxia); "multiple practice surgeries to infuse a low-molecular weight dye Fast Green confirmed consistent delivery of pump" (pg. 2709); behavioral study.

P9427 Lykkegaard,K., Larsen,P.J., Vrang,N., Bock,C., Bock,T., Knudsen,L.B. The once-daily human GLP-1 analog, liraglutide, reduces olanzapine-induced weight gain and glucose intolerance. SCHIZOPHRENIA RESEARCH 2008; 103(-1-3-):94-103. >>> Olanzapine; PBS; NaOH; lactic acid; SC; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 96) 6 animals out of 40 developed local irritation/wounds at implantation site; post op. care (carprofen); animal info (male, Sprague Dawley, 240 g.); "we used continuous subcutaneous administration of olanzapine via osmotic minipumps in order to obtain a steady-state of olanzapine with no sedation. The result of this... is an animal model that mimics the side effect profile encountered in schizophrenic patients treated with olanzapine. This may be a valuable, rapid, and inexpensive model for screening." (p. 100).

P9431 McMurray,M.S., Williams,S.K., Jarrett,T.M., Cox,E.T., Fay,E.E., Overstreet,D.H., Walker,C.H., Johns,J.M. Gestational ethanol and nicotine exposure: Effects on maternal behavior, oxytocin, and offspring ethanol intake in the rat. NEUROTOXICOLOGY AND TERATOLOGY 2008; 30(-6-):475-486. >>> Nicotine hydrogen tartrate; sodium bitartrate; Water, bacteriostatic; SC; Rat (pregnant); 2ML4; 4 weeks; Controls received mp w/ vehicle or sodium bitartrate; no stress (see pg. 474); teratology; toxicology; depedence; post op. care (bupivicaine, bacitracin zinc, polymicin B sulfate); animal info (female, Sprague Dawley, GD4); behavioral testing (maternal behaviors: pup retrieval, touch sniff flick, test building, self grooming, lay on pup; sweet preference; locomotor; exploratory behavior).

P8939 Morris,J.B., Olzinski,A.R., Bernard,R.E., Aravindhan,K., Mirabile,R.C., Boyce,R., Willette,R.N., Jucker,B.M. p38 MAPK inhibition reduces aortic ultrasmall superparamagnetic iron oxide uptake in a mouse model of atherosclerosis - MRI assessment. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2008; 28(-2-):265-271. >>> Angiotensin II; SC; Mice (knockout); 2004; 21 days; Controls received mp w/ saline; peptides; post op. care (banamine); animal info (male, ApoE -/-).

P8820 Muellner,A., Gonzenbach,R.R., Weinmann,O., Schnell,L., Liebscher,T., Schwab,M.E. Lamina-specific restoration of serotonergic projections after Nogo-A antibody treatment of spinal cord injury in rats. European Journal of Neuroscience 2008; 27(-2-):326-333. >>> Antibody, anti-Nogo-A monoclonal 11C7; antibody, anti-Nogo A monoclonal 7B12; Immunoglobulin G, mouse; CSF/CNS (intrathecal); Rat; 2ML2; 2 weeks; Controls received mp w/ IgG; post op. care (Baytril); animal info (Lewis, 2 months old, 160-180g. SCI).

Q0052 Nogueiras,R., Veyrat-Duirebex,C., Suchanek,P.M., Klein,M., Tschoep,J., Caldwell,C., Woods,S.C., Wittmann,G., Watanabe,M., Liposits,Z., Fekete,C., Reizes,O., Rohner-Jeanrenaud,F., Tschoep,M.H. Peripheral, but Not Central, CB1 Antagonism Provides Food Intake-Independent Metabolic Benefits Diet-Induced Obese Rats. Diabetes 2008; 57(-11-):2977-2991. >>> SR141716; Saline, isotonic; tween 80; CSF/CNS; SC; Rat; 2001; 6 days; Controls received mp w/ vehicle; animal info (male, Wistar, 300-350 g; male, Sprague-Dawley); post op. care (buprenorphine); comparison of IP injections vs. SC mp; CB1 antagonist; dose-response (fig. 1).

P8892 Paterson,N.E., Semenova,S., Markou,A. The effects of chronic versus acute desipramine on nicotine withdrawal and nicotine self-administration in the rat. Psychopharmacology 2008; 198(-3-):351-362. >>> Desipramine; nicotine; Saline; water; SC; Rat; 2ML1; 2ML2; 2ML4; 7, 14, 28 days; pumps replaced; dependence; animal info (male, Wistar, 300-350g.); post op. care ("antibacterial preparation"); comparison of IP injections vs. SC mp; "The results of the present study indicated that chronic, but not acute, DMI treatment attenuated the anhedonic and somatic components of nicotine withdrawal and that chronic DMI treatment significantly decreased nicotineand food-maintained responding." pg. 358.

P9434 Paterson,N.E., Balfour,D.J.K., Markou,A. Chronic bupropion differentially alters the reinforcing, reward-enhancing and conditioned motivational properties of nicotine in rats. NICOTINE&TOBACCO RESEARCH 2008; 10(-6-):995-1008. >>> Bupropion HCl; Saline; SC; Rat; 2ML2; 14 days; Dose-response (fig 4); dependence; post op. care (antibacterial lotion); animal info (male, Wistar, 300-350 g.); "minipumps were used in the present study to deliver two bupripion doses... with the aim of establishing constantly high bupropion levels that may mitigate species differences in pharmacokinetics." (p. 1006).

P9267 Reini,S.A., Dutta,G., Wood,C.E., Keller-Wood,M. Cardiac corticosteroid receptors mediate the enlargement of the ovine fetal heart induced by chronic increases in maternal cortisol. Journal of Endocrinology 2008; 198(-2-):419-427. >>> Canrenoate, potassium; RU 486; Saline; ethanol; Intrapericardial; Sheep (fetus); 2ML2; 10 days; Controls received mp w/ vehicle or sham surgery); no stress (see pg. 422); cardiovascular; post op. care (flunixamine); animal info (female, male, Ovis aries, gd 118-123); mifepristone dissolved in 47.5% EtOH, entire quantity for delivery loaded into catheter with saline in mp (similar to the Lynch coil method); endocrinology.

P9198 Rennel,E.S., Hamdollah-Zadeh,M.A., Wheatley,E.R., Magnussen,A., Schueler,Y., Kelly,S.P., Finucane,C., Ellison,D., Cebe-Suarez,S., Ballmer-Hofer,K., Mather,S., Stewart,L., Bates,D.O., Harper,S.J. Recombinant human VEGF165b protein is an effective anti-cancer agent in mice. European Journal of Cancer 2008; 44(-13-):1883-1894. >>> Vascular endothelial growth factor-165-b, recomb.; NaCl, sterile; SC; Mice; 28 days; No stress (see pg. 1888); half-life (p. 1893) 25 min in mice, 13.8-72 min in rats; cancer (colon); post op. care (Temgesic); animal info (C57/Bl6, 720 g.); silk sutures used; "no adverse effects were observed with this infusion of VEGF165b" pg. 1888; compound is an anti angiogenic isoform of VEGF.

P9677 Ritter,V., Thuering,B., Saint Mezard,P., Luong-Nguyen,N.H., Seltenmeyer,Y., Junker,U., Fournier,B., Susa,M., Morvan,F. Follicle-stimulating hormone does not impact male bone mass in vivo or human male osteoclasts in vitro. Calcified Tissue International 2008; 82(-5-):383-391. >>> Follicle stimulating hormone; Saline; SC; Mice; 2004; 30 days; Controls received mp w/ vehicle; post op. care (buprenorphine); animal info (male, C57BL/6J, 16 wks old).

P9247 Salimi,I., Friel,K.M., Martin,J.H. Pyramidal tract stimulation restores normal corticospinal tract connections and visuomotor skill after early postnatal motor cortex activity blockade. Journal of Neuroscience 2008; 28(-29-):7426-7434. >>> Muscimol; Saline, sterile; CSF/CNS (motor cortex); Cat (kitten); 2002; 2 weeks; Controls received mp w/ vehicle or no treatment; post op. care (Cefazolin, Buprenorphine); animal info (5 weeks old); behavioral testing (horizontal ladder task); cerebral palsy.

P9218 Shepherd,R.K., Coco,A., Epp,S.B. Neurotrophins and electrical stimulation for protection and repair of spiral ganglion neurons following sensorineural hearing loss. Hearing Research 2008; 242(-1-2-):100-109. >>> Brain-derived neurotrophic factor, recomb. human; Ringer's solution; albumin, guinea pig; Ear (scala tympani); Guinea-pig; 2004; 4 weeks; Controls received no treatment to contralateral cochlea; functionality of mp verified by residual volume and intact connections; peptides; post op. care (Carprofen, Baytril); tissue perfusion (scala tympani); animal info (pigmented, 400-844 g., kanamycin/furosemide deafened).

P8949 Strassburg,S., Anker,S.D., Castaneda,T.R., Burget,L., Perez-Tilve,D., Pfluger,P.T., Nogueiras,R., Halem,H., Dong,J.Z., Culler,M.D., Datta,R., Tschoep,M.H. Long-term effects of ghrelin and ghrelin receptor agonists on energy balance in rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 295(-1-):E78-E84. >>> BIM-28131; BIM-28125; ghrelin; Sodium chloride; Tween 80; serum, rat; SC; Rat; 2002; 4 weeks; Dose-response (pg. E80, fig. 1); comparison of SC injections vs. mp; pumps replaced on day 15; half-life (p. E83) ghrelin is "~10-30" min; peptide; post op. care (buprenorphine); animal info (male, Sprague Dawley, 297-316 g.); BIM-28125 and BIM-28131 are GHS-R agonists (growth hormone secretagogue receptor); pumps were primed overnight; 5% Tween 80.

P9223 Sutton,G.M., Perez-Tilve,D., Nogueiras,R., Fang,J.D., Kim,J.K., Cone,R.D., Gimble,J.M., Tschoep,M.H., Butler,A.A. The Melanocortin-3 Receptor Is Required for Entrainment to Meal Intake. Journal of Neuroscience 2008; 28(-48-):12946-12955. >>> Agouti-related peptide (82-131); CSF, artificial; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; dental cement and stay screws used; peptides; post op. care (Buprenorphine); animal info (male, B6, 8 wks old).

P8866 Terao,S., Yilmaz,G., Stokes,K.Y., Ishikawa,M., Kawase,T., Granger,D.N. Inflammatory and injury responses to ischemic stroke in obese mice. Stroke 2008; 39(-3-):943-950. >>> Leptin, mouse; SC; Mice; 1003D; 3 days; Functionality of mp verified by plasma leptin levels; peptides; post op. care (neomycin trisulfate salt hydrate); animal info (young, 5-7 wks old, ob/ob); obesity.

P9456 Ufnal,M., Sikora,M., Szczepanska-Sadowska,E. Interleukin-1 receptor antagonist reduces the magnitude of the pressor response to acute stress. Neuroscience Letters 2008; 448(-1-):47-51. >>> Interleukin-1, beta, recomb. rat; interleukin-1 receptor antagonist, recomb. rat; CSF/CNS; Rat; 2001; 5 days; Controls received mp w/ saline; cardiovascular; ALZET brain infusion kit 1 used; peptides; post op. care (benzalathine penicycline); animal info (male, Wistar-Kyoto, 12 wks old).

P9461 Whitaker,C.M., Beaumont,E., Wells,M.J., Magnuson,D.S.K., Hetman,M., Onifer,S.M. Rolipram attenuates acute oligodendrocyte death in the adult rat ventrolateral funiculus following contusive cervical spinal cord injury. Neuroscience Letters 2008; 438(-2-):200-204. >>> Rolipram; DMSO; SC; Rat; 2002; 12, 24, 72 hours; Controls received mp w/ vehicle; enzyme inhibitor (phosphodiesterase 4); multiple pumps per animal (2); post op. care (Gentozen, bacitracin zinc); animal info (female, Sprague Dawley, 228-267 g., SCI).

P9186 White,R.E., Yin,F.Q., Jakeman,L.B. TGF-alpha increases astrocyte invasion and promotes axonal growth into the lesion following spinal cord injury in mice. Experimental Neurology 2008; 214(-1-):10-24. >>> Transforming growth factor-alpha; Serum, mouse; PBS; CSF/CNS (intrathecal); Mice; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; post op. care (Baytril); animal info (female, adult, C57BL/6, 10 wks old, 17-20 g.); spinal cord injury; pumps were primed in saline at 37 degree Celsius for 24 hours; pumps were weighed before and after filling; good methods; PE-50 used for catheter.

P9183 Wilson-Gerwing,T.D., Stucky,C.L., McComb,G.W., Verge,V.M.K. Neurotrophin-3 significantly reduces sodium channel expression linked to neuropathic pain states. Experimental Neurology 2008; 213(-2-):303-314. >>> NT-3; PBS; albumin, serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Post op. care (buprenorphine); animal info (male, Wistar, young adult, 250-300 g.).

P9052 Zhang,Y., Sands,J.M., Kohan,D.E., Nelson,R.D., Martin,C.F., Carlson,N.G., Kamerath,C.D., Ge,Y.Q., Klein,J.D., Kishore,B.K. Potential role of purinergic signaling in urinary concentration in inner medulla: insights from P2Y2 receptor gene knockout mice. American Journal of Physiology-Renal Physiology 2008; 295(-6-):F1715-F1724. >>> Vasopressin, 1-deamino-8-D-arginine; Saline, sterile; SC; Mice (knockout); 1007D; 5 days; Animal info (P2Y2-R KO, wt, 2 and 5 months old); post op. care (ketorolac); nephrology.

P9146 Zhang,Z.H., Yu,Y., Kang,Y.M., Wei,S.G., Felder,R.B. Aldosterone acts centrally to increase brain renin-angiotensin system activity and oxidative stress in normal rats. American Journal of Physiology-Heart and Circulatory Physiology 2008; 294(-2-):H1067-H1074. >>> Aldosterone; RU-28318; Tempol; losartan; CSF, artificial; CSF/CNS; Rat; 2002; 1 week; Controls received mp w/ vehicle; post op. care (buprenorphine); animal info (male, Sprague Dawley, 250-300 g.); silastic tubing used; dental cement used.

P9066 Zhou,M., Wu,R.Q., Dong,W.F., Jacob,A., Wang,P. Endotoxin downregulates peroxisome proliferator-activated receptor-gamma via the increase in TNF-alpha release. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2008; 294(-1-):R84-R92. >>> Endotoxin, E. coli; IP; Rat; 2001D; 20 hours; Controls received mp w/ saline; post op. care (fluid therapy, saline, 2 ml/100 g.); animal info (male, Sprague Dawley, 280-320 g.).