References on Using ALZET® Osmotic Pumps In Space

Q0795 Nalla,A.K., Asuthkar,S., Bhoopathi,P., Gujrati,M., Dinh,D.H., Rao,J.S. Suppression of uPAR Retards Radiation-Induced Invasion and Migration Mediated by Integrin beta-1/FAK Signaling in Medulloblastoma. PLoS One 2010; 5(-9-):U240-U255. >>> RNA, small interfering, uPAR; Mice (nude); 2001; 1 week; siRNA is within a plasmid vector (pU vector).

Q0609 Chen,Q.M., Butler,D., Querbes,W., Pandey,R.K., Ge,P., Maier,M.A., Zhang,L.G., Rajeev,K.G., Nechev,L., Kotelianski,V., Manoharan,M., Sah,D.W.Y. Lipophilic siRNAs mediate efficient gene silencing in oligodendrocytes with direct CNS delivery. JOURNAL OF CONTROLLED RELEASE 2010; 144(-2-):227-232. >>> RNA, small interfering; cholesterol-conjugate siRNA; PBS; CSF/CNS (corpus callosum); Rat; 2ML1; 7 days; Controls received mp w/ vehicle; gene therapy; animal info (Male Sprague-Dawley); tissue perfusion (parenchyma); Agents are CNPase siRNA, Cholesterol-CNPas siRNA, Cholesterol-Luciferase siRNA.

Q0606 Kato,T., Natsume,A., Toda,H., Iwamizu,H., Sugita,T., Hachisu,R., Watanabe,R., Yuki,K., Motomura,K., Bankiewicz,K., Wakabayashi,T. Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells. Gene Therapy 2010; 17(-11-):1363-1371. >>> RNA, small interferring; Mice; 1007D; 1 week; Animal info (6 wks old, female NOD-SCID); MGMT-siRNA/LipoTrust complex; O6-methylguanine- DNA methyltransferase; incorrectly stated 1003D pump; cancer.

Q0517 Wang,X., Chow,F.L., Oka,T., Hao,L., Lopez-Campistrous,A., Kelly,S., Cooper,S., Odenbach,J., Finegan,B.A., Schulz,R., Kassiri,Z., Lopaschuk,G.D., Fernandez-Patron,C. Matrix Metalloproteinase-7 and ADAM-12 (a Disintegrin and Metalloproteinase-12) Define a Signaling Axis in Agonist-Induced Hypertension and Cardiac Hypertrophy. Circulation 2009; 119(-18-):2480-U112. >>> Oligonucleotide, antisense; oligonucleotide, scrambled; RNA, small interferring; angiotensin II; PBS; SC; Mice; rat; 14 days; Controls received mp w/ vehicle; animal info (MMP-7-/-, wt, 12 wks old; SHR, WKY, Sprague Dawley, 250-350 g); peptides; antisense (MMP-7); multiple pumps per animal (2); blood pressure measured indirectly using a computerized tail cuff plethysmography system.

Q0426 Cho,K.J., Kim,H.J., Park,S.C., Kim,H.W., Kim,G.W. Decisive role of apurinic/apyrimidinic endonuclease/Ref-1 in initiation of cell death. MOLECULAR AND CELLULAR NEUROSCIENCE 2010; 45(-3-):267-276. >>> Antibody, anti-APE/Ref-1, rabbit; RNA, small interfering; Saline; CSF/CNS (hippocampus); Mice; 1003D; 3 days; Controls received mp w/ vehicle; animal info (3 months old, male, ICR, 35-40 g); siRNA against APE/Ref-1.

Q0383 Querbes,W., Ge,P., Zhang,W.J., Fan,Y.P., Costigan,J., Charisse,K., Maier,M., Nechev,L., Manoharan,M., Kotelianski,V., Sah,D.W.Y. Direct CNS Delivery of siRNA Mediates Robust Silencing in Oligodendrocytes. OLIGONUCLEOTIDES 2009; 19(-1-):23-29. >>> RNA, small interferring; CSF/CNS (sorpus callosum); Rat; 2ML1; 2ML2; 3-7 days; Controls received mp w/PBS; animal info (male, Sprague Dawley); Plastics One cannula used.

Q0357 McCormack,A.L., Mak,S.K., Henderson,J.M., Bumcrot,D., Farrer,M.J., Di Monte,D.A. alpha-Synuclein Suppression by Targeted Small Interfering RNA in the Primate Substantia Nigra. PLoS One 2010; 5(-8-):U312-U319. >>> RNA, small interferring; PBS; CSF/CNS (substantia nigra); Monkey (squirrel monkey); 2004; 4 weeks; Controls received mp w/ control siRNA; animal info (feral, adult); alpha synuclein siRNA.

Q0341 Verpelli,C., Bertani,G., Cea,V., Patti,M., Bikfalvi,A., Bello,L., Sala,C. Anti-Angiogenic Therapy Induces Integrin-Linked Kinase 1 Up-Regulation in a Mouse Model of Glioblastoma. PLoS One 2010; 5(-10-):U70-U81. >>> PF4-DLR; RNA, small interfering; PBS; SC; CSF/CNS (intrathecal); Mice (nude); 2004; 10, 20 days; Controls received mp w/ vehicle; animal info (6 wks old, nude); peptides; ILK1-siRNA.

Q0213 Wang,X., Oka,T., Chow,F.L., Cooper,S.B., Odenbach,J., Lopaschuk,G.D., Kassiri,Z., Fernandez-Patron,C. Tumor Necrosis Factor-alpha-Converting Enzyme Is a Key Regulator of Agonist-Induced Cardiac Hypertrophy and Fibrosis. Hypertension 2009; 54(-3-):575-582. >>> RNA, small interfering; angiotensin II; SC; Rat; mice; 12, 14 days; Controls received mp w/ PBS; animal info (SHR, C57BL/6J male); TACE, Luc SIRNA.

P9944 Agrawal,A., Min,D.H., Singh,N., Zhu,H.H., Birjiniuk,A., von Maltzahn,G., Harris,T.J., Xing,D.Y., Woolfenden,S.D., Sharp,P.A., Charest,A., Bhatia,S. Functional Delivery of siRNA in Mice Using Dendriworms. ACS Nano 2009; 3(-9-):2495-2504. >>> RNA, small interfering; epidermal growth factor receptor; dendriworm, dye-labeled; RNA, small interfering, GFP, dendriworm, dye-labeled; CSF/CNS (intratumoral); Mice; 3, 7 days; Tissue perfusion (tumor); cancer (glioblastoma); incorrectly stated pump model 2007; Plastics One cannula used; animial info (Swiss Webster).

P9665 Lewis,J., Melrose,H., Bumcrot,D., Hope,A., Zehr,C., Lincoln,S., Braithwaite,A., He,Z., Ogholikhan,S., Hinkle,K., Kent,C., Toudjarska,I., Charisse,K., Braich,R., Pandey,R.K., Heckman,M., Maraganore,D.M., Crook,J., Farrer,M.J. In vivo silencing of alpha-synuclein using naked siRNA. Molecular Neurodegeneration 2008; 3(-;-):U1-U10. >>> RNA; small interfering; Luciferase; CSF/CNS (hippocampus); Mice; 1002; 15 days; Controls received mp w/PBS; ALZET brain infusion kit 3 used; cyanoacrylate adhesive; animal info (2 mo old, C57 BL/6 female).

P9656 Snapyan,M., Lemasson,M., Brill,M.S., Blais,M., Massouh,M., Ninkovic,J., Gravel,C., Berthod,F., Goetz,M., Barker,P.A., Parent,A., Saghatelyan,A. Vasculature Guides Migrating Neuronal Precursors in the Adult Mammalian Forebrain via Brain-Derived Neurotrophic Factor Signaling. Journal of Neuroscience 2009; 29(-13-):4172-4188. >>> Brain-derived neurotrophic factor; immunoglobulin-G-Fe; RNA, small interfering; TrkB-Fr; NaCI; IA (carotid); CSF/CNS (rostral migratory, system); Mice; 1007D; 7 days; Controls received mp w/control siRNA; animal info (2-3 mo old C57BL/J6); brain tissue distribution; ALZET mouse jugular catheter used; heparin added to BDNF.

P9654 Wu,J.S., Cheung,W.M., Tsai,Y.S., Chen,Y.T., Fong,W.H., Tsai,H.D., Chen,Y.C., Liou,J.Y., Shyue,S.K., Chen,J.J., Chen,Y.E., Maeda,N., Wu,K.K., Lin,T.N. Ligand-Activated Peroxisome Proliferator-Activated Receptor-gamma; Protects Against Ischemic Cerebral Infarction and Neuronal Apoptosis by 14-3-3 Upregulation. Circulation 2009; 119(-8-):1124-1134. >>> Peroxisome proliferator-activated receptor, gamma; RNA, small interfering; DMSO; CSF/CNS; Rat; 1007D; 72 hours; Controls received mp w/vehicle; ALZET brain infusion kit 2 used; cyanoacrylate adhesive; schemia; 14-3-3 epsilon siRNA.

P9377 Miyoshi,K., Obata,K., Kondo,T., Okamura,H., Noguchi,K. Interleukin-18-Mediated Microglia/Astrocyte Interaction in the Spinal Cord Enhances Neuropathic Pain Processing after Nerve Injury. Journal of Neuroscience 2008; 28(-48-):12775-12787. >>> Immunoglobulin G, goat; antibody, anti-interleukin-18; antibody, anti-leukin-18 receptor; interleukin-18 binding protein; RNA, small interfering; PBS; CSF/CNS (intrathecal); Rat; mice; 1003D; 2001; 3, 7 days; Controls received mp w/ vehicle or IgG or negative siRNA; peptides; animal info (male, Sprague Dawley, 200-250 g.; male, C57BL/6 wt, IL-18 -/-, 25-30 g.); siRNA targets TLR4 (toll-like receptor 4) or negative siRNA; behavioral testing (mechanical hypersensitivity, tactile allodynia).

P9320 De Toni,A., Zbinden,M., Epstein,J.A., Altaba,A.R.I., Prochiantz,A., Caille,I. Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP. Neural Development 2008; 3(-;-):U2-U13. >>> Epidermal growth factor; fibroblast growth factor, basic; RNA, small interfering; Saline, physiological; CSF/CNS; CSF/CNS (dentate gyrus); Rat; 1003D; 3 days; Controls received mp w/ control siRNA; cancer (glioblastoma); peptides; animal info (Swiss, HOP -/-, wt, adult); HOP or control siRNA was coupled to the cell permeant peptide, penetratin.

P9222 Watabe,M., Aoyama,K., Nakaki,T. A dominant role of GTRAP3-18 in neuronal glutathione synthesis. Journal of Neuroscience 2008; 28(-38-):9404-9413. >>> RNA, small interfering; cyclodextrin, methyl-B-; CSF, artificial; CSF/CNS; Mice; 1 week; 5 days; 48 hours; Controls received mp w/ vehicle or nonsilencing RNA; animal info (male, C57BL/6, 8 wks old); neurodegenerative (Parkinson's, Alzheimer's); siRNA of GTRAP 3-18 (glutamate transport associated protein) and EAAC1 (excitatory amino acid carrier 1); aCSF recipe.

P9150 Sugiyama,S., Di Nardo,A.A., Aizawa,S., Matsuo,I., Volovitch,M., Prochiantz,A., Hensch,T.K. Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity. Cell 2008; 134(-3-):508-520. >>> Otx2, protein; antibody, inhibitory otx2; RNA, small interfering, penetratin coupled; CSF/CNS (visual cortex); Mice (transgenic); 1007D; 7 days; Controls received mp w/ vehicle; animal info (Otx2 KO); Otx2 is a homeoprotein.

P8852 Wang,H.Y., Ghosh,A., Baigude,H., Yang,C.S., Qiu,L.H., Xia,X.G., Zhou,H.X., Rana,T.M., Xu,Z.S. Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression. Journal of Biological Chemistry 2008; 283(-23-):15845-15852. >>> RNA, small interfering, modified; RNA, small interfering; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice (transgenic); 1007D; 2004; 7, 28 days; 72 hours; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (Fig. 3); no stress (see pg. 15846, 15849); stability verified by 28 days in vivo (see Fig. 2); half-life (p. 15846) "short"; gene therapy; brain tissue distribution; animal info (SOD1G93A Tg); neurodegenerative (ALS); mp + catheter positioning confirmed; Target (SOD1); "when infused at disease onset at the therapeutic dose for 4 weeks, this siRNA slows disease progression without detectable adverse effects." The catheter was implanted between the L5 and L6 vertebra and connected to a primed Alzet osmotic pump with the PE50 tube. The catheter was stitched to the surface muscle, and the Alzet osmotic pumps were placed under the skin on the back of the mouse..

P8704 Tummalapalli,P., Spomar,D., Gondi,C.S., Olivero,W.C., Gujrati,M., Dinh,D.H., Rao,J.S. RNAi-mediated abrogation of cathepsin B and MMP-9 gene expression in a malignant meningioma cell line leads to decreased tumor growth, invasion and angiogenesis. INTERNATIONAL JOURNAL OF ONCOLOGY 2007; 31(-5-):1039-1050. >>> RNA, small interfering; Mice (nude); 3-4 weeks; Controls received no treatment; cancer (meningioma); animal info (athymic, nude); siRNA plasmid vectors targeting cathepsin B and matrix metalloproteinase MMP9.

P8622 Senechal,Y., Kelly,P.H., Cryan,J.F., Natt,F., Dev,K.K. Amyloid precursor protein knockdown by siRNA impairs spontaneous alternation in adult mice. Journal of Neurochemistry 2007; 102(-6-):1928-1940. >>> RNA, small interfering; RNAi buffer, isotonic; CSF/CNS (dorsal third ventricle); Mice; 1002; 2 weeks; Controls received mp w/ vehicle or control siRNA; no stress (see pg. 1934); animal info (male, C57BL/6JICO, 10-12 weeks old, 23-26 g); neurodegenerative (Alzheimer's disease); siRNA targets: amyloid precursor protein (APP), GFP, APP-mismatch, or non targeting control.

P8481 Locatelli,F., Corti,S., Papadimitriou,D., Fortunate,F., Del Bo,R., Donadoni,C., Nizzardo,M., Nardini,M., Salani,S., Ghezzi,S., Strazzer,S., Bresolin,N., Comi,G.P. Fas small interfering RNA reduces motoneuron death in amyotrophic lateral sclerosis mice. Annals of Neurology 2007; 62(-1-):81-92. >>> RNA, small interfering; CSF/CNS (intrathecal); Mice (transgenic); 2004; 4 weeks; Neurodegenerative (Amyotrophic Lateral Sclerosis); siRNA was used to silence the Fas receptor; animal info (C57BL/6, 90 days old); "Our study demonstrated that Fas silencing is able to interfere with motoneuron degeneration…providing new insights into the ALS pathogenesis and suggesting new possible strategies of molecular therapy…of ALS." p.91.

P8305 Mook,O.R., Baas,F., de Wissel,M.B., Fluiter,K. Evaluation of locked nucleic acid-modified small interfering RNA in vitro and in vivo. MOLECULAR CANCER THERAPEUTICS 2007; 6(-3-):833-843. >>> RNA, small interfering; RNA, small interfering, LNA-modified; Radio-isotopes, 3H-; SC; Mice (nude); 1007D; 7 days; 48 hours; Controls received mp w/ mismatch siRNA or saline; comparison of IV injections vs. mp; stability verified by incubation in mouse serum; half-life (p. 837) increased in LNA-modified; unmodified completely degraded in<5 hours; cancer (pancreatic); animal info (NMRI nu/nu, 8-10 weeks old); siRNA against RNA polymerase II, enhanced GFP, or mismatch.

P7393 Thakker,D.R., Natt,F., Huesken,D., van der Putten,H., Maier,R., Hoyer,D., Cryan,J.F. siRNA-mediated knockdown of the serotonin transporter in the adult mouse brain. Molecular Psychiatry 2005; 10(-8-):782-789. >>> RNA, small interfering; citalopram; RNA, mm; RNAi buffer, isotonic; CSF/CNS (dorsal third); Mice; 1002; 2 weeks; Controls received mp w/ vehicle; gene therapy; downregulation of SERT; animal info (male, BALB/C, 19-29 g); "maximally effective RNAi response requires 2 weeks of siRNA infusion." P. 783; behavioral study.

P7248 Lakka,S.S., Gondi,C.S., Dinh,D.H., Olivero,W.C., Gujrati,M., Rao,V.H., Sioka,C., Rao,J.S. Specific interference of urokinase-type plasminogen activator receptor and matrix metalloproteinase-9 gene expression induced by double-stranded RNA results in decreased invasion, tumor growth, and angiogenesis in gliomas. Journal of Biological Chemistry 2005; 280(-23-):21882-21892. >>> RNA, small interfering; RNA, small interfering, uPAR; RNA, small interfering, MMP-9; CSF/CNS; Mice (nude); 1002; 2 weeks; Controls received mp w/ PBS or empty vector; cancer (glioblastoma); siRNA is within a plasmid vector (pUM vector); antiangiogenesis; gene therapy.

P6769 Thakker,D.R., Natt,F., Huesken,D., Maier,R., Mueller,M., van der Putten,H., Hoyer,D., Cryan,J.F. Neurochemical and behavioral consequences of widespread gene knockdown in the adult mouse brain by using nonviral RNA interference. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-49-):17270-17275. >>> RNA, small interfering; GBR 12909; RNAi buffer; CSF/CNS (dorsal third ventricle); Mice; 1007D; 1002; 1,2 weeks; Controls received mp w/ vehicle; antisense (EGFP, DAT, scrambled sequence); Plastics One cannula used; "Results highlight the temporal effects of siRNA, requiring a constant minipump-mediated infusion in the dorsal third ventricle for a stable and bilateral knockdown of gene expression in the brain." (p. 17275); gene therapy.

P6720 Gondi,C.S., Lakka,S.S., Dinh,D.H., Olivero,W.C., Gujrati,M., Rao,J.S., Rao,J.S. RNAi-mediated inhibition of cathepsin B and uPAR leads to decreased cell invasion, angiogenesis and tumor growth in gliomas. ONCOGENE 2004; 23(--):8486-8496. >>> RNA, small interfering; EV/SV vector; PBS; CSF/CNS (intratumoral); Mice (nude); ; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioma); gene therapy; antiangiogenesis; siRNA (cathepsin B); pump model not stated (0.25 ul/hr); "...local intracranial delivery of pcu using mini-osmotic pumps effectively inhibited human malignant glioma growth.".

P6618 Lakka,S.S., Gondi,C.S., Yanamandra,N., Olivero,W.C., Dinh,D.H., Gujrati,M., Rao,J.S. Inhibition of cathepsin B and MMP-9 gene expression in glioblastoma cell line via RNA interference reduces tumor cell invasion, tumor growth and angiogenesis. ONCOGENE 2004; 23(-27-):4681-4689. >>> RNA, small interfering; human cytomegalovirus promoter; Empty vector; CSF/CNS; Mice; 2004; 5 weeks; Cancer (glioblastoma); siRNA against mmp-9 and cathepsin; antiangiogenesis; gene therapy.

P5995 Dorn,G., Patel,S., Wotherspoon,G., Hemmings-Mieszczak,M., Barclay,J., Natt,F.J.C., Martin,P., Bevan,S., Fox,A., Ganju,P., Wishart,W., Hall,J. siRNA relieves chronic neuropathic pain. NUCLEIC ACIDS RESEARCH 2004; 32(-5-):1-6. >>> RNA, small interfering; oligonucleotide, antisense; Saline; CSF/CNS (intrathecal); Rat; 6-7 days; Controls received mp w/ missense siRNA; no stress (see pg. 4); antisense (P2X3); gene therapy; siRNA (P2X3); pain research.

P5821 Isacson,R., Kull,B., Salmi,P., Wahlestedt,C. Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain. Acta Physiol Scand 2003; 179(-2-):173-177. >>> RNA, small interfering; Water, RNAse-free; CSF/CNS (caudate putamen); Rat; 1003D; 3 days; Controls received mp w/ vehicle; siRNA (dopamine D, receptors); ALZET brain infusion kit used.