Studies Including Information on the Stability of Agents in ALZET^® Osmotic Pumps

P8979 Susic,D., Zhou,X.Y., Frohlich,E.D., Lippton,H., Knight,M. Cardiovascular effects of prorenin blockade in genetically spontaneously hypertensive rats on normal and high-salt diet. American Journal of Physiology-Heart and Circulatory Physiology 2008; 295(-3-):H1117-H1121. >>> PRAM-1; Water, distilled; SC; Rat; 2ML4; 4, 8 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 4 weeks; stability verified; cardiovascular; peptides; animal info (male, SHR, 20 wks old); PRAM-1 is a custom synthesized prorenin inhibitor; "no degradation products of the peptide were observed".

P8928 Tejeda,M., Gaal,D., Hullan,L., Csuka,O., Schwab,R., Szokolocz,O., Keri,G. A comparison of the tumor growth inhibitory effect of intermittent and continuous administration of the somatostatin structural derivative TT-232 in various human tumor models. Anticancer Research 2006; 26(-4B-):3011-3015. >>> TT-232; Water, distilled; acetic acid; sodium acetate; mannitol; SC; Mice (SCID); 2002; 14, 28 days; Comparison of IP, SC injections vs. SC mp; stability verified by 3 weeks at 37 degree Celsius by HPLC analysis for agent + degradation products; cancer (epidermoid carcinoma, breast carcinoma); peptides; animal info (female, SCID, 22-24 g.); somatostate structural derivative; "The comparative experiments (injection and infusion) confirmed that continuous treatments and long-term administration were associated with the best treatment responses" (p. 3014); "Continuous infusion administration of TT-232 significantly inhibited the growth of the tumor when compared with intraperitoneal (ip) and subcutaneous (sc) intermittent injection treatments." (p. 3011).

P8852 Wang,H.Y., Ghosh,A., Baigude,H., Yang,C.S., Qiu,L.H., Xia,X.G., Zhou,H.X., Rana,T.M., Xu,Z.S. Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression. Journal of Biological Chemistry 2008; 283(-23-):15845-15852. >>> RNA, small interfering, modified; RNA, small interfering; PBS; CSF/CNS (intrathecal, subarachnoid space); Mice (transgenic); 1007D; 2004; 7, 28 days; 72 hours; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (Fig. 3); no stress (see pg. 15846, 15849); stability verified by 28 days in vivo (see Fig. 2); half-life (p. 15846) "short"; gene therapy; brain tissue distribution; animal info (SOD1G93A Tg); neurodegenerative (ALS); mp + catheter positioning confirmed; Target (SOD1); "when infused at disease onset at the therapeutic dose for 4 weeks, this siRNA slows disease progression without detectable adverse effects.".

P8775 Roa,J., Vigo,E., Garcia-Galiano,D., Castellano,J.M., Navarro,V.M., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2008; 294(-6-):E1088-E1096. >>> Kisspeptin-10; Leptin; Saline, physiological; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by response duration and examination; stability verified by persistent FSH response; peptide; animal info (female, Wistar, 205-275 g., adult, 110-120g., 30 days old).

P8639 Palaniyandi,S.S., Nagai,Y., Watanabe,K., Ma,M., Veeraveedu,P.T., Prakash,P., Kamal,F.A., Abe,Y., Yamaguchi,K., Tachikawa,H., Kodama,M., Aizawa,Y. Chymase inhibition reduces the progression to heart failure after autoimmune myocarditis in rats. EXPERIMENTAL BIOLOGY AND MEDICINE 2007; 232(-9-):1213-1221. >>> TY-51469; Phosphate buffer; NaOH; IV (jugular); Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; dose-response (fig. 2); stability verified by study in rat plasma at 40oC for 1 hour; enzyme inhibitor (chymase); cardiovascular; animal info (male, Lewis, 9 wks old).

P8606 Hagg,T., Baker,K.A., Emsley,J.G., Tetzlaff,W. Prolonged local neurotrophin-3 infusion reduces ipsilateral collateral sprouting of spared corticospinal axons in adult rats. Neuroscience 2005; 130(-4-):875-887. >>> Neurotrophin-3, recomb. human; PBS; gentamicin; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (fig. 4); stability verified by activity assay of residual mp content after 14 days in vivo; ALZET brain infusion kit used; peptides; post op. care (Penicillin G, buprenorphine); animal info (Sprague-Dawley, 3 months old, 200-220 grams, partial resection of corticospinal tract); schematic of mp p. 877 fig 2.

P8437 Henke,E., Perk,J., Vider,J., de Candia,P., Chin,Y., Solit,D.B., Ponomarev,V., Cartegni,L., Manova,K., Rosen,N., Benezra,R. Peptide-conjugated antisense oligonucleotides for targeted inhibition of a transcriptional regulator in vivo. NATURE BIOTECHNOLOGY 2008; 26(-1-):91-100. >>> Oligonucleotide, antisense, peptide conjugated; oligonucleotide, phosphorothioate antisense; peptide, F3; TBS; SC; Mice (nude); mice; 1007D; 1002; 14, 21 days; Controls received mp w/ vehicle or control peptide and oligo in non-conjugated form, or rcId1-PCAO; comparison of IV injections vs. mp; pumps replaced after 14 days; no stress (see pg. 95, 97); stability verified after 24 hours in 37 C, plasma up to 28 days in 37 C saline; antisense (Id1, rcId1); cancer (breast, lung carcinoma); peptides; toxicology; animal info (female, nude NCR; male, C57J/B6); antisense oligos covalently coupled to address peptide that targets tumor endothelial cells; "no adverse effects on treated mice have been observed." (p. 97).

P8419 Jiang,G.L., Nieves,A., Im,W.B., Old,D.W., Dinh,D.T., Wheeler,L. The prevention of colitis by E prostanoid receptor 4 agonist through enhancement of epithelium survival and regeneration. Journal of Pharmacology and Experimental Therapeutics 2007; 320(-1-):22-28. >>> AGN205203; DMSO; saline, normal; SC; Mice; 1002; 9 days; Controls received mp w/ vehicle; metabolic&chemical stability verified; animal info (femal, C57BL/6, 8 wks old); EP4 agonist.

P8406 Zeevalk,G.D., Manzino,L., Sonsalla,P.K., Bernard,L.P. Characterization of intracellular elevation of glutathione (GSH) with glutathione monoethyl ester and GSH in brain and neuronal cultures: Relevance to Parkinson's disease. Experimental Neurology 2007; 203(-2-):512-520. >>> Glutathione, monoethyl ester; MPP+; Saline; SC; CSF/CNS; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; dose-response (fig. 6); stability verified by incubation for 0-28 days at 37 celsius, assessed by HPLC; half-life (pg. 515) 10-14 hours in culture; brain tissue distribution; toxicology; animal info (Sprague-Dawley, 300g.); neurodegenerative (Parkinson's disease).

P8305 Mook,O.R., Baas,F., de Wissel,M.B., Fluiter,K. Evaluation of locked nucleic acid-modified small interfering RNA in vitro and in vivo. MOLECULAR CANCER THERAPEUTICS 2007; 6(-3-):833-843. >>> RNA, small interfering; RNA, small interfering, LNA-modified; Radio-isotopes, 3H-; SC; Mice (nude); 1007D; 7 days; 48 hours; Controls received mp w/ mismatch siRNA or saline; comparison of IV injections vs. mp; stability verified by incubation in mouse serum; half-life (p. 837) increased in LNA-modified; unmodified completely degraded in<5 hours; cancer (pancreatic); animal info (NMRI nu/nu, 8-10 weeks old); siRNA against RNA polymerase II, enhanced GFP, or mismatch.

P8302 Zhang,X.F., Connolly,C., Duquette,M., Lawler,J., Parangi,S. Continuous administration of the three thrombospondin-1 type 1 repeats recombinant protein improves the potency of therapy in an orthotopic human pancreatic cancer model. Cancer Letters 2007; 247(-1-):143-149. >>> Thrombospondin-1, three type 1 repeats; PBS; SC; Mice (SCID); 1007D; 7 days; Controls received mp w/ vehicle; comparison of IP injections vs. mp; stability verified by incubation in pump, 7 days; cancer (pancreatic); peptides; animal info (female, SCID, 4-6 weeks old); antiangiogenesis therapy; 3TSR; "Continuous administration of 3TSR reduced dosage and improved therapeutic potency." p. 146.

P8289 Banach-Petrosky,W., Ouyang,X.S., Gao,H., Nader,K., Ji,Y., Suh,N., DiPaola,R.S., bate-Shen,C. Vitamin D inhibits the formation of prostatic intraepithelial neoplasia in Nkx3.1; Pten mutant mice. Clinical Cancer Research 2006; 12(-19-):5895-5901. >>> Vitamin D3, 1,25-dihydroxy-; Propylene glycol; SC; Mice; 2004; 1-4 months; Controls received mp w/ vehicle; long-term study; pumps replaced every 4 weeks; stability verified by serum calcium levels; cancer (prostate); animal info (4-7 months old, male).

P8284 Yazdani,U., German,D.C., Liang,C.L., Manzino,L., Sonsalla,P.K., Zeevalk,G.D. Rat model of Parkinson's disease: Chronic central delivery of 1-methyl-4-phenylpyridinium (MPP+). Experimental Neurology 2006; 200(-1-):172-183. >>> MPP+; Saline-iodine; CSF/CNS; Rat; 2ML4; 14 days; Stability verified by HPLC, 28 days; animal info (male, Sprague-Dawley, 300-350 grams); neurodegenerative (Parkinson's Disease); "This study presents a chronic model of PD in rats that encompasses the desirable components of current chronic models while reducing their less desirable aspects." P. 181.

P8266 Urushitani,M., bou Ezzi,S., Julien,J.P. Therapeutic effects of immunization with mutant superoxide dismutase in mice models of amyotrophic lateral sclerosis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-7-):2495-2500. >>> Antibody, mouse anti hSOD1; antibody, rabbit polyclonal anit-hSOD1-FITC; Saline; CSF/CNS; Mice; 2004; 16, 28 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; stability verified by Western Blot w/ residual volume AB after 18 days infusion; brain tissue distribution; animal info (G93A SOD1, 85 days old); neurodegenerative (ALS).

P8236 Lecanu,L., Greeson,J., Papadopoulos,V. Beta-amyloid and oxidative stress jointly induce neuronal death, amyloid deposits, gliosis, and memory impairment in the rat brain. Pharmacology 2006; 76(-1-):19-33. >>> Amyloid protein, beta 42; ferrous sulfate; buthionine sulfoximine; CSF, artificial; water, distilled; CSF/CNS; Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; stability verified by 14 days in mp at 37 C; animal info (male, Long-Evans, 3-4 month old, 300-325 grams); neurodegenerative (Alzheimer's disease).

P8145 Grommes,C., Landreth,G.E., Sastre,M., Beck,M., Feinstein,D.L., Jacobs,A.H., Schlegel,U., Heneka,M.T. Inhibition of in vivo glioma growth and invasion by peroxisome proliferator-activated receptor gamma agonist treatment. MOLECULAR PHARMACOLOGY 2006; 70(-5-):1524-1533. >>> Pioglitazone; DMSO; PBS; CSF/CNS (intratumoral); Rat; 2ML4; 3, 6, 9, 14, 21 days; Controls received mp w/ vehicle; comparison of oral admin. vs. mp; no stress (see p.1532); stability verified by in vitro incubation at 37 celsius for 21 days; cancer (glioma); ALZET brain infusion kit used; animal info (Sprague-Dawley, 200-250g.); 0.1% DMSO; Antineoplastic; tissue perfusion (tumor).

P8096 Gosain,A., Matthies,A.M., Dovi,J.V., Barbul,A., Gamelli,R.L., DiPietro,L.A. Exogenous pro-angiogenic stimuli cannot prevent physiologic vessel regression. Journal of Surgical Research 2006; 135(-2-):218-225. >>> Vascular endothelial growth factor 164, recomb.; platelet-dreived growth factor; fibroblast growth factor-2; Wound site; Mice; 2002; 11 days; Controls received mp w/ PBS; functionality of mp verified by VEGF levels in wound sponges, residual volume; stability verified by activity of residual VEGF in endothelial cell cord formation assay (fig.4); cardiovascular; peptides; animal info (female, BALB/c, 8-9 wks old, implanted sponge wounds); "The activity of VEGF isolated from the pump was comparable to fresh recombinant VEGF 164, confriming that the recombinant growth factors present in the mini-osmotic pump retain robust biological activity." (p.221).

P8052 Brown,J.R., Fuster,M.M., Li,R.X., Varki,N., Glass,C.A., Esko,J.D. A disaccharide-based inhibitor of glycosylation attenuates metastatic tumor cell dissemination. Clinical Cancer Research 2006; 12(-9-):2894-2901. >>> Disaccharide, AcGnG-NM; DMSO; propylene glycol; SC; Mice; 2004; 2, 4 weeks; Controls received mp w/ vehicle; stability verified by incubation in mouse serum; cancer (lung carcinoma, B16Bl6 melanoma); animal info (c57BL/6 Es1(e); 8-12 wk old, lung metastases); 50% DMSO.

P8015 Vrang,N., Madsen,A.N., Tang-Christensen,M., Hansen,G., Larsen,P.J. PYY(3-36) reduces food intake and body weight and improves insulin sensitivity in rodent models of diet-induced obesity. Am.J.Physiol Regul.Integr.Comp Physiol 2006/8; 291(-2-):R367-R375. >>> Peptide YY (3-36); Saline, isotonic; SC; Mice; rat; 2004; 2ML4; 28 days; Stability verified by in vitro test, degradation was<0.5%/day; peptides; animal info (C57BL/6, DIO and rats, Wistar and Sprague-Dawley); gut hormone; SC administration of PYY (3-36) for 28 days reduced body weight and improved glycemic control.

P7973 Bartolomucci,A., La Corte,G., Possenti,R., Locatelli,V., Rigamonti,A.E., Torsello,A., Bresciani,E., Bulgarelli,I., Rizzi,R., Pavone,F., D'Amato,F.R., Severini,C., Mignogna,G., Giorgi,A., Schinina,M.E., Elia,G., Brancia,C., Ferri,G.L., Conti,R., Ciani,B., Pascucci,T., Dell'Omo,G., Muller,E.E., Levi,A., Moles,A. TLQP-21, a VGF-derived peptide, increases energy expenditure and prevents the early phase of diet-induced obesity. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-39-):14584-14589. >>> TLQP-21, synthetic; CSF, artificial; CSF/CNS; Mice; 1002; 14 days; Controls received mp w/ vehicle; stability verified by HPLC after 14 days at 37 C; ALZET brain infusion kit 1 used; peptides; animal info (male, CD1); obesity.

P7956 Di,Cristo G., Berardi,N., Cancedda,L., Pizzorusso,T., Putignano,E., Ratto,G.M., Maffei,L. Requirement of ERK activation for visual cortical plasticity. Science 2001/6/22; 292(-5525-):2337-2340. >>> U0126; PD 98059; SB203580; CSF/CNS (visual cortex); Rat; 1 week; Controls received mp w/ vehicle; stability verified by inhibition of ERK activation by picrotoxin; enzyme inhibitor (p38 MAP kinase); animal info (Dark-reared, P28); tissue perfusion (visual cortex).

P7942 Berni,R., Cacciani,F., Zaniboni,M., Savi,M., Bocchi,L., Lapucci,S., Razzetti,R., Pastore,F., Musso,E., Stilli,D. Effects of the alpha 2-adrenergic/DA2-dopaminergic agonist CHF-1024 in preventing ventricular arrhythmogenesis and myocyte electrical remodeling, in a rat model of pressure-overload cardiac hypertrophy. Journal of Cardiovascular Pharmacology 2006; 47(-2-):295-302. >>> CHF-1024; Saline; ascorbic acid; SC; Rat; 2ML4; 1 month; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; dose-response (table 1); stability verified for at least 1 month (pg. 296); cardiovascular; post op. care (gentamicin); animal info (male, Wistar, 6 months old); "a clear dose dependency was found in CHF-1024 plasma levels, indicating a good performance of the osmotic pumps in drug release." (p. 299).

P7940 Ambati,J., Gragoudas,E.S., Miller,J.W., You,T.T., Miyamoto,K., Delori,F.C., Adamis,A.P. Transscleral delivery of bioactive protein to the choroid and retina. Invest Ophthalmol.Vis.Sci. 2000/4; 41(-5-):1186-1191. >>> Immunoglobulin G, FITC-, rabbit; antibody, monoclonal mouse anti-ICAM-1; immunoglobulin, mouse IgG2a; Eye (superotemporal scleral surface, transscleral); Rabbit; 2001D; 2ML4; 3,5,13,20,28 days; 24 hours; Controls received mp w/ control Ab; functionality of mp verified by fluorescence in occular tissues and plasma mAb levels; stability verified by efficacy experiments, FITC linkage timing; half-life (p. 1188), 3 days; ALZET brain infusion kit used; animal info (dutch-belted, pigmented); "We have developed a minimally invasive transscleral drug delivery modality that can deliver therapeutic concentrations of bioactive proteins to the choroid and retina without significant systemic absorption or tissue damage." (P. 1186-87).

P7900 Thompson,E.L., Murphy,K.G., Patterson,M., Bewick,G.A., Stamp,G.W.H., Curtis,A.E., Cooke,J.H., Jethwa,P.H., Todd,J.F., Ghatei,M.A., Bloom,S.R. Chronic subcutaneous administration of kisspeptin-54 causes testicular degeneration in adult male rats. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2006; 291(-5-):E1074-E1082. >>> Kisspeptin-54; Saline; SC; Rat; 2001D; 2001; 2002; 1, 2, 3, 13 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; comparison of SC injection vs. mp; no stress (see pg. E1078); stability verified by 14 days in mp in 37 celsius saline (see fig. 2); good methods pg. E1075; peptides; post op. care (flucloxacillin, amoxicillin); animal info (male, Wistar, 275-325g.); mp primed for 3hrs. (2001D) or overnight (2001, 2002) in 37 celsius saine; "animals were healthy throughout the study period. No adverse behaviors were observed in any of the treatment groups at any point during the study." (pg. E1078).

P7844 Royo,N.C., Conte,V., Saatman,K.E., Shimizu,S., Belfield,C.M., Soltesz,K.M., Davis,J.E., Fujimoto,S.T., McIntosh,T.K. Hippocampal vulnerability following traumatic brain injury: a potential role for neurotrophin-4/5 in pyramidal cell neuroprotection. European Journal of Neuroscience 2006; 23(-5-):1089-1102. >>> Neurotrophin-4/5; PBS; BSA; CSF/CNS (somatosensory cortex); Rat; 1007D; 1 week; Controls received mp w/ vehicle; no stress (see pg. 1095); stability verified by activity after 14 days in mp at 37 celsius; ALZET brain infusion kit II used; peptides; animal info (male, Sprague-Dawley, 350-400g., traumatic brain injury by lateral fluid percussion); mp primed 18 hours at 37 celsius.

P7843 Yamaguchi,Y., Miyashita,H., Tsunekawa,H., Mouri,A., Kim,H.C., Saito,K., Matsuno,T., Kawashima,S., Nabeshima,T. Effects of a novel cognitive enhancer, spiro[imidazo[1,2-a]pyridine-3,2- indan]-2(3H)-one (ZSET1446), on learning impairments induced by amyloid-beta (1-40) in the rat. Journal of Pharmacology and Experimental Therapeutics 2006; 317(-3-):1079-1087. >>> Amyloid protein, beta (1-40); amyloid protein, beta (40-1); Acetonitrile; trifluoroacetic acid; CSF/CNS; Rat; 2002; 15 days; Controls received mp w/ vehicle, or AB (40-1); stability verified by testing in solution at 37 celsius (pg. 1080); neurodegenerative (alzheimer's disease); animal info (male, Wistar, 7 wk. old); "An animal model induced by chronic intracerebroventricular (i.c.v.) infusion of AB is particularly attractive for evaluation of drugs for AD because change is similar to those known for AD patients" (pg. 1080); 35% acetonitrile used.

P7817 Igwe,O.J. Agents that act by different mechanisms modulate the activity of protein kinase C beta II isozyme in the rat spinal cord during peripheral inflammation. Neuroscience 2006; 138(-1-):313-328. >>> L-703606 oxalate; dextrorphan tartrate; oligodeoxynucleotide, phosporothioate antisense; phosporothioate missense; Propylene glycol; water, sterile; saline; oligofectamine; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig 1); stability verified; antisense (protein kinase C, beta); animal info (male, Sprague-Dawley, 200-300 grams); "mini osmotic pumps were used to administer all agents in order to reduce animal handling after CFA-induced inflammation." (p. 315).

P7757 Winaver,J., Ovcharenko,E., Rubinstein,I., Gurbanov,K., Pollesello,P., Bishara,B., Hoffman,A., Abassi,Z. Involvement of Rho kinase pathway in the mechanism of renal vasoconstriction and cardiac hypertrophy in rats with experimental heart failure. American Journal of Physiology-Heart and Circulatory Physiology 2006; 290(-5-):H2007-H2014. >>> Y-27632; Saline; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; stability verified by assesment in saline and plasma at 37celsius for up to 500 min.; enzyme inhibitor (Rho Kinase); cardiovascular; animal info (male, Wistar, 280-350g., CHF).

P7702 Guarnieri,M., Biser-Rohrbaugh,A., Tyler,B.M., Gabikian,P., Bunton,T.E., Wu,Q.Z., Weingart,J., Carson Sr,B.S. Toxicity of intracranial and intraperitoneal O6-benzyl guanine in combination with BCNU delivered locally in a mouse model. Cancer Chemother.Pharmacol. 2002/11; 50(-5-):392-396. >>> Benzylguanine, 06-; Propylene glycol; ethanol; IP; CSF/CNS (parietal lobe); Mice; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (table 2); comparison of IP injection vs. mp; stability verified by 14 days at 37 C, UV spectra analysis; good methods (p. 393); cancer (glioma brain); toxicology; animal info (female, BALB/C, 6-12 weeks old); 10% ETOH.

P7563 Ikegami,T., Nakamura,M., Yamane,J., Katoh,H., Okada,S., Iwanami,A., Watanabe,K., Ishii,K., Kato,F., Fujita,H., Takahashi,T., Okano,H.J., Toyama,Y., Okano,H. Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury. European Journal of Neuroscience 2005; 22(-12-):3036-3046. >>> Chondroitinase ABC; Saline, physiological; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001; 1 week; Controls received mp w/ heat inactivated agent; stability verified by ALZAID chemical compability test kit for 7 days; animal info (adult, female, Sprague-Dawley 230-250 g); mp primed overnight in 37 C saline; spinal cord injury.

P7529 Tanaka,K., Takemoto,T., Sugahara,K., Okuda,T., Mikuriya,T., Takeno,K., Hashimoto,M., Shimogori,H., Yamashita,H. Post-exposure administration of edaravone attenuates noise-induced hearing loss. European Journal of Pharmacology 2005; 522(-1-3-):116-121. >>> Edaravone; saline; Water; NaOH; Ear (cochlea); Guinea-pig; 2002; 12 hours; Controls received no treatment to left ear; pumps replaced, mp w/ saline used then replaced with mp with agent; stability verified for 24 hours; post op. care (antibiotic ointment); animal info (male, Hartley, 300-450 g); tissue perfusion (cochlea).

P7519 Kennedy,D.J., Vetteth,S., Periyasamy,S.M., Kanj,M., Fedorova,L., Khouri,S., Kahaleh,M.B., Xie,Z.J., Malhotra,D., Kolodkin,N.I., Lakatta,E.G., Fedorova,O.V., Bagrov,A.Y., Shapiro,J.I. Central role for the cardiotonic steroid marinobufagenin in the pathogenesis of experimental uremic cardiomyopathy. Hypertension 2006; 47(-3-):488-495. >>> Marinobufagenin; Rat; 2004; 4 weeks; Controls received sham operation; functionality of mp verified by agent plasma levels; comparison of partial nephrectomy vs. mp; stability verified for 4 weeks at 37 C by comparable inhibition of 86Rb uptake; cardiovascular; animal info (male, Sprague-Dawley).

P7500 Lotinun,S., Sibonga,J.D., Turner,R.T. Evidence that the cells responsible for marrow fibrosis in a rat model for hyperparathyroidism are preosteoblasts. Endocrinology 2005; 146(-9-):4074-4081. >>> Parathyroid hormone, human 1-34; thymidine, methyl -3H; Sodium chloride; HCl; serum, rat; ethanol; SC; Rat; 1, 2, 4 weeks; 1, 3, 5 days; Controls received mp w/ vehicle; functionality of mp verified by serum PTH; pumps replaced after 2 weeks; stability verified; peptides; multiple pumps per animal (2); animal info (female, Sprague-Dawley, 260 g); 2% ethanol.

P7497 Elitok,S., Brodsky,S.V., Patschan,D., Orlova,T., Lerea,K.M., Chander,P., Goligorsky,M.S. Cyclic arginine-glycine-aspartic acid peptide inhibits macrophage infiltration of the kidney and carotid artery lesions in apo-E-deficient mice. American Journal of Physiology-Renal Physiology 2006; 290(-1-):F159-F166. >>> Peptide, CRGD; peptide, CRAD; SC; Mice; 1002; 2004; 14, 28 days; Controls received mp w/ PBS; functionality of mp verified by platelet aggregation inhibitory properties and residual volume; dose-response (fig. 3); stability verified by mass spec of residual volume; half-life (p. F160); peptides; animal info (female, C57BL6, 7 weeks, 290 g); cartoid artery ligation.

P7467 Oldroyd,S.D., Miyamoto,Y., Moir,A., Johnson,T.S., El Nahas,A.M., Haylor,J.L. An IGF-I antagonist does not inhibit renal fibrosis in the rat following subtotal nephrectomy. American Journal of Physiology-Renal Physiology 2006; 290(-3-):F695-F702. >>> Insulin-like growth factor I, receptor antagonist; SC; Rat; 2002; 2004; 10, 90 days; Controls received mp w/ vehicle, or sham operation; dose-response (fig. 3); long-term study; pumps replaced every 4 weeks; stability verified by storage in mp for 4 weeks; peptides; animal info (male, wistar, chronic kidney disease, 200-300 g); IGF-I receptor antagonist (JB3); mp primed 4 hr in 37C isotonic saline.

P7394 Guarnieri,M., Carson,B.S., Khan,A., Penno,M., Jallo,G.I. Flexible versus rigid catheters for chronic administration of exogenous agents into central nervous system tissues. Journal of Neuroscience Methods 2005; 144(-2-):147-152. >>> Doxorubicin; carboblatin; Dextrose solution; saline; CSF/CNS (parenchyma); Rat; 2001; ; Animal info (F, Fischer, 220 g); Functionality of mp verified by pump examination, post infusion; stability verified by previous studies, 14 days; good methods (p. 148); cancer (glioma); brain tissue distribution; Plastics One cannula used (stainless steel); "replacement of rigid infusion tubes [cannulae] with flexible tubing increases the reliability of local CNS drug infusions. Rigid catheters [cannulae] may allow backflow of the infused drug along the path of the catheters into the subdural space." (P. 147).

P7267 Fluiter,K., Frieden,M., Vreijling,J., Rosenbohm,C., de Wissel,M.B., Christensen,S.M., Koch,T., Orum,H., Baas,F. On the in vitro and in vivo properties of four locked nucleic acid nucleotides incorporated into an anti-H-Ras antisense oligonucleotide. CHEMBIOCHEM 2005; 6(-6-):1104-1109. >>> Oligodeoxynucleotide, antisense; oligodeoxynucleotide, amino-LNA antisense; oligodeoxynucleotide, thio-LNA antisense; oligodeoxynucleotide, LNA antisense; oligodeoxynucleotide, missense; SC; Mice (nude); 1002; 11 days; 48 hours; Controls received mp w/ saline; dose-response (fig. 4); no stress (see pg. 1106); stability verified by incubation in human serum; antisense (H-Ras); cancer (prostate).

P7265 Ji,B.X., Li,M.W., Budel,S., Pepinsky,R.B., Walus,L., Engber,T.M., Strittmatter,S.M., Relton,J.K. Effect of combined treatment with methylprednisolone and soluble Nogo-66 receptor after rat spinal cord injury. European Journal of Neuroscience 2005; 22(-3-):587-594. >>> Nogo-66 receptor (27-310) ecto-Fc; immunoglobulin G, rat; PBS; CSF/CNS (intrathecal); Rat; 2004; 28 days; Controls received mp w/ vehicle rat IgG; functionality of mp verified by residual volume; stability verified by analysis of remaining mp contents; post op. care (buprenorphine/buprenex, ampicillin); spinal cord injury.

P7264 Boesen,E.I., Anderson,W.P., Kett,M.M. Role of endothelin in noradrenaline-induced hypertension in rats. Journal of Hypertension 2005; 23(-5-):987-993. >>> Noradrenalin; TAK-044; heparin; Saline, heparinized; ascorbic acid; saline; IV (vena cava); Rat; 2ML2; 24 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (fig. 2); pumps replaced at day 10; no stress (see pg. 989); stability verified by acute pressor activity after 14 days at 37 degrees celsius in mp; cardiovascular; multiple pumps per animal (2); 10 IU/ml of heparin.

P7137 Baker,S.A., Baker,K.A., Hagg,T. D3 dopamine receptors do not regulate neurogenesis in the subventricular zone of adult mice. NEUROBIOLOGY OF DISEASE 2005; 18(-3-):523-527. >>> DPAT, 7-hydroxy-U-99194A maleate; Saline; CSF/CNS; CSF/CNS (neostriatum); Rat; mice; 1002; 14 days; Controls received mp w/ vehicle; dose-response; comparison of IP injections vs. mp; stability verified by BrdU labeling; cyanoacrylate adhesive used.

P7105 Roberson,D.W., Alosi,J.A., Cotanche,D.A. Direct transdifferentiation gives rise to the earliest new hair cells in regenerating avian auditory epithelium. Journal of Neuroscience Research 2004; 78(-4-):461-471. >>> Uridine, bromodeoxy-; Ear (cochlea); Bird (chicken); 2002; 3-11 days; Controls received untreated contralateral ear; functionality of mp verified by (epithelial immunohistochemistry); stability verified by epithelial immunohistochemistry; tissue perfusion (cochlea).

P7099 Benn,S.C., Ay,I., Bastia,E., Chian,R.J., Celia,S.A., Pepinsky,R.B., Fishman,P.S., Brown,RH Jr, Francis,J.W. Tetanus toxin fragment C fusion facilitates protein delivery to CNS neurons from cerebrospinal fluid in mice. Journal of Neurochemistry 2005; 95(-4-):1118-1131. >>> Toxin, tetanus fragment C; tempol, toxin tetanus fragment C; CSF/CNS; Mice; 2001; 7 days; Stability verified by incubation at 37C for 7 days; brain tissue distribution; post op. care (bupivicaine and betadine in wound); animal info (C57BL/6, male, 25-30 g); dental cement used for cannula adhesion, agent also know as TTC; compounds were also distributed to further brain sites such as the hypothalamus and the thalamus.

P7077 Lefranc,F., Mijatovic,T., Mathieu,V., Rorive,S., Decaestecker,C., Debeir,O., Brotchi,J., Van Ham,P., Salmon,I., Kiss,R. Characterization of gastrin-induced proangiogenic effects in vivo in orthotopic U373 experimental human glioblastomas and in vitro in human umbilical vein endothelial cells. Clinical Cancer Research 2004; 10(-24-):8250-8265. >>> Gastrin-17; Saline; CSF/CNS (intratumoral); Rat (nude); 1002; 7 days; Controls received mp w/ vehicle; stability verified; cancer (glioma).

P7068 Harris-White,M.E., Balverde,Z., Lim,G.P., Kim,P., Miller,S.A., Hammer,H. Role of LRP in TGFbeta2-mediated neuronal uptake of A beta and effects on memory. Journal of Neuroscience Research 2004; 77(-2-):217-228. >>> Amyloid protein, beta; transforming growth factor-B2; receptor-associated protein; HEPES buffer; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; stability verified by incubation at 37 degree Celsius for 2,4 weeks; peptides; RAP is a LRP (lipoprotein receptor-related protein) antagonist; memory.

P7008 DeKosky,S.T., Abrahamson,E.E., Taffe,K.M., Dixon,C.E., Kochanek,P.M., Ikonomovic,M.D. Effects of post-injury hypothermia and nerve growth factor infusion on antioxidant enzyme activity in the rat: implications for clinical therapies. Journal of Neurochemistry 2004; 90(-4-):998-1004. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Rat; 2002; 1,3 days; Controls received mp w/ vehicle; stability verified by increased ChAT activity.

P6953 Thaete,L.G., Kushner,D.M., Dewey,E.R., Neerhof,M.G. Endothelin and the regulation of uteroplacental perfusion in nitric oxide synthase inhibition-induced fetal growth restriction. PLACENTA 2005; 26(-2-3-):242-250. >>> L-NAME; A-127722; Saline; ethyl alcohol; NaOH; water; propylene glycol; SC; Rat (pregnant); 2ML1; 1,4,7 days; Functionality of mp verified by residual volume; stability verified by prior experiment (24 days); multiple pumps per animal (2); drugs infused in separate pumps; A-127722 (ETA receptor antagonist) was dissolved in 20% alcohol, 40% PG and 0.04 M of NaOH.

P6921 Love,D.A., Lietman,S.A. The effect of osteogenic protein-1 dosing regimen on ectopic bone formation. Clinical Orthopaedics and Related Research 2004; (-423-):264-267. >>> Osteogenic protein-1; Lactic acid; Bone (matrix); Rat; 1002; 14 days; comparison of bolus injections vs. mp; stability verified by in vitro incubation study; silastic tubing used to target the SC implanted bone matrix; tissue perfusion (bone matrix).

P6894 Tejeda,M, Gaal,D., Csuka,O., Keri,G.Y. Growth Inhibitory Effect of the Somatostatin Structural Derivative (TT-232) on Leukemia Models. Anticancer Research 2005; 25(--):325-330. >>> TT-232; Acetic acid; sodium acetate; water; mannitol; IV; SC; Mice; 2002; 14, 28 days; Dose-response (p. 328, fig 1); comparison of IP/SC injections vs. SC/IV mp; pumps replaced at day 14 for 28 day group; stability verified, 37 degrees Celsius for over 3 weeks; cancer (leukemia); TT-232 is a novel somatostatin analog; "The IV infusion for 28 days resulted in 82% growth inhibition." (p. 328); "The infusion of TT-232 by ALZET osmotic minipump resulted in 70-80% tumor growth inhibition and 20% tumor free survival." (p. 329); "…serial injections represent significant stress to the animals…" "To reduce and eliminate the above mentioned problem [stress] we used an ALZET osmotic minipump…" "Infusion from inserted ALZET minipumps maintains a constant drug level, resulting in a well defined, consistent pattern of drug exposure throughout the period of drug administration." "These studies suggest that TT-232 is a potent inhibitor of leukemia tumor in vitro and in vivo and suggest infusion treatment as a beneficial application in clinical practice." (p. 330); oligopeptide; enzyme inhibitor (tyrosine kinase); animal info (CBA/ca, immunosuppressed, female).

P6599 Guimaraes,M.A.M., Nikolovski,J., Pratt,L.M., Greive,K., Comper,W.D. Anomalous fractional clearance of negatively charged Ficoll relative to uncharged Ficoll. American Journal of Physiology-Renal Physiology 2003; 285(-6-):F1118-F1124. >>> Ficoll, 3H-carboxymethyl; Rat; 2001; 7 days; Functionality of mp verified by GFR and UFR; comparison of IV injections vs. mp; stability verified by ion exchange chromatography of 3H-carboxymethyl ficoll collected on day 7.

P6559 Skuladottir,G.V., Olason,P.I., Jonsson,L., Skarphedinsson,J.O., Eriksdottir,V.H., Atlason,P.T., Franzson,L., Schioeth,H.B. Hyperphagia modifies FA profiles of plasma phospholipids, plasma FFA, and adipose tissue TAG. Lipids 2003; 38(-11-):1127-1132. >>> HS024; CSF, artificial; CSF/CNS; Rat; 2004; 8,28 days; Controls received mp w/ vehicle; stability verified by HPLC&MS weekly; ALZET brain infusion kit; peptides; Melanocortin receptor antagonist.

P6539 Locher,C.P., Witt,S.A., Ashlock,B.M., Levy,J.A. Evaluation of genetic immunization adjuvants to improve the effectiveness of a human immunodeficiency virus type 2 (HIV-2) envelope DNA vaccine. DNA and Cell Biology 2004; 23(-2-):107-110. >>> DNA, plasmid; Gene, HIV-2 gpl40; SC; Mice; 2004; 28 days; 28 day stability verified by agarose gel electrophoresis; immunology; different methods of immunization used; genetic immunization; DNA did not adhere to the osmotic minipump after 1 month since DNA concentration remained stable and intact.

P6497 Tejeda,M., Gaal,D., Barna,K., Csuka,O., Keri,G. The antitumor activity of the somatostatin structural derivative (TT-232) on different human tumor xenografts. Anticancer Research 2003; 23(-5A-):4061-4066. >>> TT-232; Acetic acid; sodium acetate; water, distilled; mannitol; SC; Mice; 2002; 14,28 days; Dose-response (fig.2); comparison of daily SC injections vs. mp; pumps replaced at day 14; stability verified over 3 weeks; cancer (prostate, breast, colon, melanoma, leukemia); peptides; "a significant antitumor activity of TT-232 was observed following long-term infusion using osmotic minipumps" (p. 4064).

P6378 Monassier,L., Greney,H., Thomas,L., Bousquet,P. Chronic treatment with rilmenidine in spontaneously hypertensive rats: Differences between two schedules of administration. Journal of Cardiovascular Pharmacology 2004; 43(-3-):394-401. >>> Rilmenidine; Saline; IP; Rat; 2002; 1 month; Comparison of IP injections vs. IP mp; pumps replaced at 15 days; stability verified (30 days at 37 degrees Celsius); cardiovascular; antihypertensive.

P6373 Souweidane,M.M., Occhiogrosso,G., Mark,E.B., Edgar,M.A. Interstitial infusion of IL13-PE38QQR in the rat brain stem. Journal of Neuro-oncology 2004; 67(-3-):287-293. >>> Interleukin-13,PE38QQR; Saline; serum albumin; CSF/CNS (brain stem); Rat; 2001D; 24 hours; Tissue perfusion (brain stem); stability verified (7 days at 37 degrees Celsius); good methods p. 288; cancer; cyanoacrylate adhesive; pump incorrectly labeled as a 2001; IL13-PE38QQR is a tumor specific, chimeric cytotoxin; 30 g Plastics One Cannula used; vinyl catheter tubing from DURECT used.

P6329 Hong,M.K., Kent,K.M., Mehran,R., Mintz,G.S., Tio,F.O., Foegh,M., Wong,S.C., Cathapermal,S.S., Leon,M.B. Continuous Subcutaneous Angiopeptin Treatment Significantly Reduces Neointimal Hyperplasia in a Porcine Coronary In-Stent Restenosis Model. Circulation 1997; 95(-2-):449-454. >>> Angiopeptin; SC; Pig; 2ML1; 1 week; Controls received saline via catheter; comparison of dispatch catheter (30 min.); stability verified by plasma levels; good methods p.450; cardiovascular; post op. care (aspirin, ticlopidine); "continuous subcutaneous angiopeptin treatment was the optimal mode of angiopeptin therapy + resulted in maximal reduction in neointimal hyperplasia after stent implantation" (p.452).

P6328 Cabelli,R.J., Shelton,D.L., Segal,R.A., Shatz,C.J. Blockade of Endogenous Ligands of TrkB Inhibits Formation of Ocular Dominance Columns. Neuron 1997; 19(--):63-76. >>> Immunoglobulin G, TrkA; immunoglobulin, TrkB; immunoglobulin G, TrkC; NT-4/5; NT-3; BSA; PBS, sterile; CSF/CNS (visual cortex); Cat; ferret; 2002; 2,3 weeks; 2 days; Controls received mp w/ vehicle, or IgG protein fusions unrelated to Trk; comparison of CSF/CNS injections vs. mp; stability verified by Ab immunostaining of brain sections; peptides; post op. care (7 days oral amoxicillin); 3 week infusions had a flow rate of approx. 0.33 ul/hr, "the reduced flow rate (~0.33 ul/hr) was achieved by coating ~1/3 of the surface area of the minipump with hot paraffin prior to filling") p. 74).

P6327 Bozkurt,B., Kribbs,S.B., Clubb,F.J., Michael,L.H., Didenko,V.V., Hornsby,P.J., Seta,Y., Oral,H., Spinale,F.G., Mann,D.L  Circulation 1998; 97(--):1382-1391. >>> Tumor Necrosis factor-; Albumin, rat serum; IP; Rat; 2002; 15 days; Controls received mp w/ vehicle; functionality of mp verified by tail blood samples; no stress (see pg. 1387); stability verified by tail blood samples; cardiovascular; peptides; TNF-a was recombinant human.

P6245 Froidevaux,S., Eberle,A.N. Homologous regulation of melanocortin-1 receptor (MC1R) expression in melanoma tumor cells in vivo. JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH 2002; 22(-1-4-):111-121. >>> Melanocyte-stimulating hormone, a-; Citric Acid; sodium chloride; SC; Mice (SCID); 1002; 3-14 days; Comparison of IV injections vs. mp; stability verified by HPLC analysis after 2 week peptide incubation @ 37ºC; cancer (melanoma); peptides.

P6192 Dunaiski,V., Belford,D.A. Contribution of circulating IGF-I to wound repair in GH-treated rats. GROWTH HORMONE&IGF RESEARCH 2002; 12(-6-):381-387. >>> Insulin-like growth factor I; Acetic acid; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; stability verified by daily animal weight gain; imunology; peptides.

P6188 Debeir,T., Marien,M., Chopin,P., Martel,J.C., Colpaert,F., Raisman-Vozari,R. Protective effects of the alpha2-adrenoceptor antagonist, dexefaroxan, against degeneration of the basalocortical cholinergic system induced by cortical devascularization in the adult rat. Neuroscience 2002; 115(-1-):41-53. >>> Dexefaroxan; Water, distilled; SC; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; stability verified by independent exp for 28d; neurodegenerative (Alzheimer's disease); neurology.

P6129 Seeds,N.W., Basham,M.E., Ferguson,J.E. Absence of tissue plasminogen activator gene or activity impairs mouse cerebellar motor learning. Journal of Neuroscience 2003; 23(-19-):7368-7375. >>> Plasminogen activator inhibitor-1; tissue plasminogen activator, STOP; CSF, artificial; CSF/CNS (cerebellum); Mice; 1007D; 7 days; Controls received mp w/ vehicle; stability verified by amidolytic assays (tpa-STOP); good methods (p. 7369); half-life (p. 7369, 145 hours); ALZET brain infusion kit 1 used; recovery period; PAI-1 was recomb. mutant; "pump implantation and subsequent infusion of either aCSF or PAI-1 did not impair the mice's ability to perform a previously learned motor task." p. 7372; behavioral study.

P6115 Wang,H., White,R., Leenen,F.H.H. Stimulation of brain Na(+) channels by FMRFamide in Dahl SS and SR rats. American Journal of Physiology-Heart and Circulatory Physiology 2003; 285(-5-):H2013-H2018. >>> FMRFamide; CSF, artificial; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; blood pressure taken; stability verified by keeping solution at room temp. for 1 wk; cardiovascular; peptides; CSF production rate mentioned (p. H2014); FMRFamide dissolved in aCSF with a pH of 7.4 and stored at -20 celsius was found to be stable for 1 yr of storage.

P5850 Richardson,R.D., Omachi,K., Kermani,R., Woods,S.C. Intraventricular insulin potentiates the anorexic effect of corticotropin releasing hormone in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2002; 283(-6-):R1321-R1326. >>> Insulin, porcine; CSF, artifical; CSF/CNS (third ventricle); Rat; 2002; 14 days; Controls received mp w/ vehicle; stability verified by past experiment >11 days stable p.322; peptides; two cannulae used, one in the 3rd ventricle&the other in the right ventricle; 3 day recovery; two cannula systems used so that acute administration of compounds (via lateral ventricle) would not interfere w/ continuous infusion of a second solution (via the third ventricle).

P5832 Miller,A.L., Prieskorn,D.M., Altschuler,R.A., Miller,J.M. Mechanism of electrical stimulation-induced neuroprotection: effects of verapamil on protection of primary auditory afferents. Brain Research 2003; 966(-2-):218-230. >>> Verapamil; Perilymph, artifical; Ear (cochlea); guinea-pig; 2002; 6 weeks; Pumps replaced every two weeks; 2 week stability verified by high pressure liquid chromatography p. 220; good methods p.220; 3 day recovery post implant; tissue perfusion (cochlea).

P5819 Giussani,C., Carrabba,G., Pluderi,M., Lucini,V., Pannacci,M., Caronzolo,D., Costa,F., Minotti,M., Tomei,G., Villani,R., Carroll,R.S., Bikfalvi,A., Bello,L. Local intracerebral delivery of endogenous inhibitors by osmotic minipumps effectively suppresses glioma growth in vivo. Cancer Research 2003; 63(-12-):2499-2505. >>> Platelet factor-4; MMP-2 (hemopexin fragment); PBS; SC; CSF/CNS (intratumoral); Mice; 2004; 28 or 94 days; Controls received mp w/ PBS; tissue perfusion (tumor); dose-response (p.2502); comparison of IP injections SC systemic mp infusion vs. local intracranial mp infusion; long-term study; pumps replaced after 28 days in some groups; stability verified by in vitro assay; cancer (glioma); Angiogenesis inhibitors; COOH terminal fragment of PF-4 used; In vitro activity of agents confirmed for up to 16 days; "In conclusion, our data demonstrates that local intracerebral delivery of endogenous inhibitors by osmotic minipumps is a very effective modality for the treatment of aggressive tumors..." (p.2505); tissue perfusion (tumor);.

P5817 Shinkai,N., Takasuna,K., Takayama,S. Inhibitory effects of formoterol on lipopolysaccharide-induced premature delivery through modulation of proinflammatory cytokine production in mice. REPRODUCTION 2003; 125(-2-):199-203. >>> Endothelin-1; IV (femoral); Rat; 2002; ; Funcionality of mp verified by pump assessment; 2-week stability verified by previous studies by other groups; ET-1 plasma levels measured.

P5688 Abella,A., Marti,L., Camps,M., Claret,M., Fernandez-Alvarez,J., Gomis,R., Guma,A., Viguerie,N., Carpene,C., Palacin,M., Testar,X., Zorzano,A. Semicarbazide-sensitive amine oxidase/vascular adhesion protein-1 activity exerts an antidiabetic action in Goto-Kakizaki rats. Diabetes 2003; 52(-4-):1004-1013. >>> Benzylamine hydrochloride; SC; Rat; 2 weeks; 2-week stability verified by in vitro glucose transport assay; diabetes..

P5670 Eckardt,H., Bundgaard,K.G., Christensen,K.S., Lind,M., Hansen,E.S., Hvid,I. Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis. Journal of Orthopaedic Research 2003; 21(-2-):335-340. >>> Vascular endothelial growth factor; vascular endothelial growth factor R2/FC chimera; Phosphate buffer; BSA; Bone (tibia); Rabbit; 2ML2; 14 days; Controls received mp w/ vehicle; tissue perfusion (osteotomy site); replacement therapy (distraction osteogenesis); stability verified by in vitro assay for 2 weeks at 37 C. (p. 336);.

P5619 Yin,D.H., Xu,H.P., He,Y.L., Kirkovsky,L.I., Miller,D.D., Dalton,J.T. Pharmacology, pharmacokinetics, and metabolism of acetothiolutamide, a novel nonsteroidal agonist for the androgen receptor. Journal of Pharmacology and Experimental Therapeutics 2003; 304(-3-):1323-1333. >>> Testosterone propionate; acetothiolutamide; bicalutamide, R-; PEG 300; SC; Rat; 2002; 2 weeks; Controls received mp w/vehicle; functionality of mp verified by residual volume; replacement therapy (orchidectomy); comparison of bolus IV injections vs. mp (p. 1327); stability verified by preliminary experiments (14 days at 37 C. of acetothiolutamide; half life (p. 1327) acetothiolutamide (26 min); bicalutamide is a nonsteroidal androgen; acetothioluthamide is a nonsteroidal androgen receptor ligand; agents in seperate pumps for seperate groups..

P5575 Gangula,P.R.R., Dong,Y.L., Wimalawansa,S.J., Yallampalli,C. Infusion of pregnant rats with calcitonin gene-related peptide (CGRP)( 8-37), a CGRP receptor antagonist, increases blood pressure and fetal mortality and decreases fetal growth. Biology of Reproduction 2002; 67(-2-):624-629. >>> Calcitonin gene-related peptide (8-37); saline; SC; rat (pregnant); 2ML2; ; controls received mp with saline; stability verified by mass spectrometry for 14 days (p. 625); peptides; agent is a potent vasodilator.

P5567 Yamamoto,M., Fisher,J.E., Gentile,M., Seedor,J.G., Leu,C.T., Rodan,S.B., Rodan,G.A. The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats. Endocrinology 1998/3; 139(-3-):1411-1419. >>> Echistatin; Saline; SC; Rat; mice; 2002; 2ML1; 2, 4 weeks; 2002 pumps replaced after 2 weeks in some groups; 2ML1 pumps replaced weekly for total 4-week infusion; 14-day stability verified by in vitro echistatin binding assay; peptides; 2002 pumps used in mice; 2ML1 pumps used in rats; Echistatin is a snake venom peptide with high affinity for beta3 integrins.

P5559 Brynne,L., Paalzow,L.K., Karlsson,M.O. Mechanism-based modeling of rebound tachycardia after chronic l- propranolol infusion in spontaneous hypertensive rats. J.Pharmacol.Exp.Ther. 1999/8; 290(-2-):664-671. >>> Propranolol; Saline; SC; Rat; 2ML2; 11 days; Functionality of mp verified by plasma levels; pK data available (p. 667); propranolol 11-day stability verified by testing samples before and after implantation; one group received multiple pumps per animal (2); "the minipumps were used to obtain a constant beta-receptor blockage..." (p. 665).

P5476 Carson,BS Sr, Wu,Q.Z., Tyler,B., Sukay,L., Raychaudhuri,R., Dimeco,F., Clatterbuck,R.E., Olivi,A., Guarnieri,M. New approach to tumor therapy for inoperable areas of the brain: chronic intraparenchymal drug delivery. Journal of Neuro-oncology 2002; 60(-2-):151-158. >>> Carboplatin; bis-chlorethyl nitrosourea; Propylene glycol; dextrose; CSF/CNS (brain stem); Rat; 2001; 7 days; Controls received mp w/ vehicle; stability verified by HPLC (p. 151); half-life (p. 154) for BCNU is<1 hr; cancer; brain tissue distribution; study evaluated effects of various infusion rates from 1-10 ul/hr to the brainstem and cortex.

P5391 Sahu,A. Resistance to the satiety action of leptin following chronic central leptin infusion is associated with the development of leptin resistance in neuropeptide Y neurones. Journal of Neuroendocrinology 2002; 14(-10-):796-804. >>> Leptin; PBS; CSF/CNS; Rat; 2002; 28 days; Controls received mp w/ vehicle; pumps replaced 14 days; stability verified by plasma leptin levels; peptides; 7-day aCSF infusion followed by 28-day leptin infusion followed by another period of aCSF infusion.

P5337 Slot,L.A.B., Tarayre,J.P., Koek,W., Ribet,J.P., Colpaert,F.C. Experimental conditions for the continuous subcutaneous infusion of four central analgesics in rats. Pharmacology Biochemistry and Behavior 2002; 72(-4-):943-951. >>> Morphine HCl; Ketamine HCl; Imipramine HCl; gabapentin; Water, distilled; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ saline; no stress (see pg. 947); 14 day stability verified by HPLC on all agents; the site of pump placement was massaged daily to avoid tissue adherance; opioid and non-opioid analgesics; body weight gain information provided.

P5336 Schmiedt,R.A., Lang,H.N., Okamura,H., Schulte,B.A. Effects of furosemide applied chronically to the round window: A model of metabolic presbyacusis. Journal of Neuroscience 2002; 22(-21-):9643-9650. >>> Apomorphine; Saline; SC; Mice; 28 days; Controls received mp w/ vehicle; comparison of sc bolus injections vs. mp; 20-day stability verified by HPLC (p.368); neurodegenerative (Parkinson's disease); "The neurorescue effect of continuous subcutaneous infusion of apomorphine is particularly promising from a clinical standpoint." (p.372).

P5270 Suga,M., Maclean,A.A., Keshavjee,S., Fischer,S., Moreira,J.M., Liu,M. RANTES plays an important role in the evolution of allograft transplant- induced fibrous airway obliteration. Am.J Respir.Crit Care Med. 2000/11; 162(-5-):1940-1948. >>> Antibody, polyclonal anti-rat RANTES; Immunoglobulin G, rabbit; RANTES; Saline; PBS; BSA; Tracheal graft; Rat; 1007D; 2002;; 7, 14, 24 days; Controls received mp w/ vehicle; pumps replaced after 14 days; 14-day stability verified by RANTES ELISA (p.1944); Immunology; peptides; transplantation; RANTES is a chemokine; concentration of RANTES in mp measured before and after delivery (p.1945).

P5269 Qiu,J., Cai,D., Dai,H., McAtee,M., Hoffman,P.N., Bregman,B.S., Filbin,M.T. Spinal axon regeneration induced by elevation of cyclic AMP. Neuron 2002/6/13; 34(-6-):895-903. >>> H89; Saline; CSF/CNS (intrathecal); Rat; 1 week; Controls received mp w/ vehicle; 1 week stability verified (results not shown); enzyme inhibitor; H89 is a protein kinase A inhibitor.

P5207 Hamilton,B.S., Doods,H.N. Chronic application of MTII in a rat model of obesity results in sustained weight loss. Obes.Res 2002/3; 10(-3-):182-187. >>> MTII; Saline; SC; Rat; 2ML4; 17 or 28 days; Controls received mp w/ vehicle; stability verified by HPLC (p.183); MTII is a non-specific melanocortin agonist; pumps left in animal for aprox. 38 days; effects of MTII were noted up until day 38.

P5186 Gordon,T., Boyd,J.G., Gordon,T. A dose-dependent facilitation and inhibition of peripheral nerve regeneration by brain-derived neurotrophic factor. European Journal of Neuroscience 2002; 15(--):613-626. >>> Brain-derived neurotropic factor; PBS; Tween 20; CSF/CNS (tibial nerve); Rat; 2ML4; 28 days; Controls received gelfoam or mp w/ saline; tissue perfusion (tibial nerve); stability verified by in vitro assay on embryonic chick neurons (p. 614); good methods (p. 614-5); peptides; silastic tubing used; graphic showing nerve perfusion (p. 615); "pump, nerve cuff and drug delivery catheter did not interfere with regeneration of the TIB motor axons" (p. 617).

P5046 Scharstuhl,A., Glansbeek,H.L., van Beuningen,H.M., Vitters,E.L., van der Kraan,P.M., Van Den Berg,W.B. Inhibition of endogenous TGF-beta during experimental osteoarthritis prevents osteophyte formation and impairs cartilage repair. Journal of Immunology 2002; 169(--):507-514. >>> Transforming Growth Factor-B2, soluble receptor; Polymyxin B; IP; mice; 1007D; 2002; 7,14 or 21 days; Arthritis; controls received empty pumps or mp w/ vehicle; functionality of mp verified by residual aspiration; pumps replaced after 14 days; stability verified by ELISA after infusion (p. 508, 510); soluble TGF-B RII selectively inhibits transforming growth factor-B; peptides; polymyxin B included as endotoxin inhibitor; 2002 pumps used for 7 and 14 day administration, 21-day group received a 1007D pump for final 7 days.

P5040 Rohner-Jeanrenaud,F., Craft,L.S., Bridwell,J., Suter,T.M., Tinsley,F.C., Smiley,D.L., Burkhart,D.R., Statnick,M.A., Heiman,M.L., Ravussin,E., Caro,J.F. Chronic central infusion of cocaine- and amphetamine-regulated transcript (CART 55-102): effects on body weight homeostasis in lean and high -fat-fed obese rats. International Journal of Obesity 2002; 26(--):143-149. >>> Endostatin; Sodium citrate; Sodium phosphate; IP; mice (SCID); mice; 2001; 6 days; controls received mp w/ vehicle; functionality of mp verified by serum endostatin levels; dose-response (p.7673, Fig. 4); comparison of SC and IP bolus injections vs. continuous infusion w/ mp; pumps replaced every 7 days; endostatin stability verified by in vitro biologic activity after 1-7 day implantation; half-life (p. 7669) of endostation in mice after SC bolus is ~10 hrs.; cancer; angiogenesis inhibitor; recomb. human endostatin used; continuous administration of human endostatin resulted in increased efficacy and an 8-10-fold decrease in dose required to achieve the same effect as daily bolus administration.

P4957 Peeling,J., Del Bigio,M.R., Corbett,D., Green,A.R., Jackson,D.M. Efficacy of disodium 4-[(tert-butylimino)methyl]benzene-1,3-disulfonat e N-oxide (NXY-059), a free radical trapping agent, in a rat model of hemorrhagic stroke. Neuropharmacology 2001; 40(--):433-439. >>> NXY-059; Saline; SC; rat; 2ML1; 3 days; controls received mp w/ vehicle; functionality of mp verified by plasma NXY-059 levels; dose-response (p. 436 section 3.1); stability verified by checking concentration of remaining drug p. 436); multiple pumps per animal (2); NXY-059 traps free radicals in the brain and may reduce brain injury in hemorrhagic stroke; ischemia (cerebral).

P4902 Jelic,M., Pecina,M., Haspl,M., Kos,J., Taylor,K, Maticic,D, McCartney,J, Yin,S., Rueger,D., Vukicevic,S. Regeneration of articular cartilage chondral defects by osteogenic protein-1 (bone morphogenetic protein-7) in sheep. Growth Factors 2001; 19(--):101-113. >>> Osteogenic protein-1; Acetate buffer; knee (articular cavity); sheep; 2002; 14 days; controls received mp w/ vehicle; tissue perfusion (knee joint defect); functionality of mp verified by aspiration of residual volume after explant; stability verified by in vitro compatibility tests, ELISA, HPLC; good methods (in vitro bio-compatibility table 1 p. 106); OP-1 is also known as BMP-7. Pump was stapled to femoral shaft, PE-60 catheter tubing perfused knee joint; "Results of this study suggest that a recombinant BMP delivered via a mini-osmotic pump into the joint fluid stimulate the repair of articular cartilate defects in sheep." p. 106;.

P4873 Marti,L., Abella,A., Carpene,C., Palacin,M., Testar,X., Zorzano,A. Combined treatment with benzylamine and low dosages of vanadate enhances glucose tolerance and reduces hyperglycemia in streptozotocin-induced diabetic rats. Diabetes 2001; 50(--):2061-2068. >>> Benzylamine; PBS; SC; rat; 2 weeks; controls received sham operation; functionality of mp verified by plasma insulin levels; dose response (table 4 p. 2065-66); comparison of SC injections vs. mp; stability verified by preliminary studies-- benzylamine stable for 2 weeks; rats were diabetic; benzylamine encourages glucose uptake..

P4771 Perez-Romero,A., De Lama,M.A.R., Granados,B., Vara,E., Gonzalez,I.V., Ariznavarreta,C., Tresguerres,J.A.F. Growth hormone response to long-term GH-RH administration in lambs. Journal of Physiology and Biochemistry 2000; 56(-2-):107-115. >>> Transforming growth factor-B1; Bone (tibia); Rabbit; 2ML4; 4 weeks;; Controls received mp w/ vehicle; tissue perfusion (osteotomy site via catheter); functionality of mp verified by aspirate residual volume after pump removal; no stress (see pg. 620); stability verified by fibroblast cell growth compared w/ fresh solution 7, 14, 21; peptides; dosage of TGF-b1 was 0, 10, 20, or 40 mg/day; delayed drug delivery for 7 days;.

P4764 Larsen,P.J, Vrang,N, Petersen,P.C, Kristensen,P  Obesity Research 2000; 8(-8-):590-596. >>> CART (42-89);; PBS;; CSF/CNS;; rat;; 2001; 2002;; 7, 10 days;; Controls received mp w/ vehicle; dose-response (graph p. 592); pulsed delivery 24 hr. delay for recovery; stability verified by aspire 5 mL of pump contents, inject ICV into naive animals to verify biological activity; peptides; ALZET brain infusion kit used; recombinant CART (42-89) is a hypothalamic neuropeptide; CART (42-89) stands for cocaine-amphetamine-regulated transcript; low dose was 4.8 mg/day for 7 days, high dose was 12 mg/day for 10 days..

P4672 Nickola,T.J., Ignatowski,T.A., Spengler,R.N. Antidepressant drug administration modifies the interactive relationship between a2-adrenergic sensitivity and levels of TNF in the rat brain.. Journal of Neuroimmunology 2000; 107(--):50-58. >>> Tumor necrosis factor-a; CSF, artificial;; CSF/CNS;; rat;; 14 days;; Stability verified by WEHI-13 VAR fibroblast cylotoxicity assay; peptides; ALZET brain infusion kit used; recomb. rat TNF used;.

P4590 Senzaki,H, Gluzband,Y.A, Pak,P.H, Crow,M.T, Janicki,J.S., Kass,D.A  Circ.Res. 1998; 82(--):503-512. >>> Angiotensin II; Acetic acid; dog;; 4-7 days;; controls received mp w/angiotensin II only; functionality of mp verified by plasma levels; stability verified by HPLC; peptides, cardiovascular;.

P4586 Lippuner,K, del Pozo,E, MacKenzie,A., Jaeger,Ph. Long-term systemic administration of human recombinant interleukin-1B induces a dose-dependent fall in circulating parathyroid hormone in rats.. Horm.Res. 1999; 51(--):74-77. >>> Interleukin-1, beta;; Water, distilled;; SC;; rat;; 2002;; 14 days;; controls received mp w/vehicle; functionality of mp verified by corticosterone plasma levels; stability verified; peptides; recomb. human Interleukin-1 beta..

P4492 Zhang,M, Luo,B, Chen,S-J, Abrams,G.A, Fallon,M.B  Am.J.Physiol.(Gastrointest.Liver Physiol.40) 1999; 277(--):G944-G952. >>> Endothelin-1;; Saline;; IV (femoral);; rat;; 2002;; 2 weeks;; controls received mp w/vehicle; functionality of mp verified by residual volume; stability verified by RIA after 2 weeks in vitro incubation in the pumps; peptides;.

P4428 Mattar,S.G, Hanson,S.R,Pierce G.F,Chen,C, Hughes,J.D, Cook,J,E, Shen,C, Noe,B.A, Suwyn,C.R, Scott,J.R, Lumsden,A.B  Journal of Surgical Research 1996; 60(--):339-344. >>> Fibroblast growth factor; saporin;; Sodium citrate; NaCl; EDTA;; IA (carotid);; dog;; 2002;; 14 days;; controls received mp w/vehicle; stability verified for 14 days by cellular assay; peptides; Saporin- FGF2 conjugate was infused; pump placed externally; Saporin is a potent ribosome-inactivating protein..

P4216 Yang,Z.-J., Meguid,M.M., Chai,J.-K., Chen,C., Oler,A. Bilateral hypothalamic dopamine infusion in male Zucker rat suppresses feeding due to reduced meal size. Pharmacol.Biochem.Behav. 1997; 58(-3-):631-635. >>> Dopamine HCl; Sodium metabisulfite; CSF/CNS (hypothalamus); rat; 2002; 13 days; controls received mp w/vehicle; stability verified by HPLC after 13 days; two pumps implanted per animal; bilateral cannula used.

P4150 Markowska,A.L., Mooney,M., Sonntag,W.E. Insulin-like growth factor-1 ameliorates age-related behavioral deficits. Neuroscience 1998; 87(-3-):559-569. >>> Insulin-like growth factor I; CSF, artificial;; CSF/CNS; rat; 2002; 28 days; controls received mp w/vehicle; functionality of mp verified by residual volume; pumps replaced after 14 days; four-week stability verified by bioassay of residual IGF-1; cannula placement confirmed at necropsy; peptides.

P4131 Yoshihara,F., Nishikimi,T., Horio,T., Yutani,C., Takishita,S., Matsuo,H., Ohe,T., Kangawa,K. Chronic infusion of adrenomedullin reduces pulmonary hypertension and lessens right ventricular hypertrophy in rats administered monocrotaline. Eur.J.Pharmacol. 1998; 355(--):33-39. >>> Adrenomedullin, synthetic rat; Saline; SC; rat; 2ML4; 21 days; controls received mp w/saline; functionality of mp verified by plasma levels; stability verified by measuring concentration at time of explant; adrenomedullin is a potent vasorelaxant peptide; incorrectly assessed pump delivery by weighing pumps at time of explant; peptides.

P4107 Benhar,I., Reiter,Y., Pai,L.H., Pastan,I. Administration of disulfide-stabilized FV-immunotoxins B1(dSFV)-PE38 and B3(dSFV)-PE38 by continuous infusion increases their efficacy in curing large tumor xenografts in nude mice. Int.J.Cancer 1995; 62(--):351-355. >>> B1(dSFV)-PE38; B3(dSFV)-PE38; PBS; IP; mice (nude); 1007D; 6 days; controls received mp w/vehicle; functionality of mp verified by immunotoxin blood levels; comparison of IV bolus injections vs. mp; stability verified by 37 C incubation up to 14 days and assay; cancer; immunology; agents are dSFV-immunotoxins; "These results show that continuous infusion results in a significantly larger...therapeutic index, and...may be a more effective method delivering the dSFV-immunotoxins" (p. 355); good stability data.

P4092 Ilyin,S.E., Sonti,G., Gayle,D., Plata-Salaman,C.R. Regulation of brain interleukin-1b (IL-1b) system mRNAs in response to pathophysiological concentrations of IL-1b in the cerebrospinal fluid. J.Mol.Neuroscience 1996; 7(--):169-181. >>> Interleukin-1, beta; Interleukin-1, beta heat inactivated; Saline, sterile; BSA; CSF/CNS; rat; 2001; 7 days; stability verified by previous studies; a 29 g stainless steel obturator used to ensure cannulae patency; peptides; recomb. human IL-1 used.

P4031 Switzer,S.K., Wallner,B.P., Briner,T.J., Sunshine,G.H., Bourque,C.R., Luqman,M. Bolus injection of aqueous antigen leads to a high density of T-cell-receptor ligand in the spleen, transient T-cell activation and anergy induction. Immunology 1998; 94(--):513-522. >>> Antigen, moth cytochrome C peptide; PBS; mice (transgenic); 1007D; 1 week; functionality of mp verified by residual volume; comparison of bolus injections vs. mp; stability verified by amino acid analysis and sequence analysis; good methods (p. 514); immunology; peptides.

P4017 Nishimura,M., Ohtsuka,K., Sakamoto,M., Nanbu,A., Takahashi,H., Yoshimura,M. Roles of brain angiotensin II and C-type natriuretic peptide in deoxycorticosterone acetate-salt hypertension in rats. J.Hypertens. 1998; 16(--):1175-1185. >>> Natriuretic peptide, C-type, 22; candesartan; CSF, artificial; CSF/CNS; rat; 2001; 7 days; controls received mp w/vehicle; stability verified by assaying bioactivity after 7-day incubation; cannula placement verified at end of study by the infusion of methylene blue solution; angiotensin II receptor antagonists; CNP-22 is C-type natriuretic peptide; cardiovascular; antihypertensive; candesartan is CV-11974.

P4000 Ehrenhofer,C., Opdebeeck,J.P. The effects of continuous and intermittent delivery of antigens of Boophilus microplus on the development of murine antibodies. Vet.Parasitol. 1995; 59(--):263-273. >>> Antigens, parasite; SC; mice; 2002; 4 weeks; functionality of mp verified by assaying residual amount of protein content; comparison of intermittent injections vs. mp; pumps replaced every 2 weeks; no stress (see p. 267); stress/adverse reaction: pus and occasional adhesion (p. 267); stability verified by HPSEC and ELISA after 14 days; antigen immunization; immunology.

P3939 Webb,R.L., Navarrete,A.E., Davis,S. Effects of valsartan and hydrochlorothiazide alone and in combination on blood pressure and heart rate in conscious-telemetered spontaneously hypertensive rats (SHR). Am.J.Hypertens. 1998; 11(--):59-65. >>> Valsartan; Hydrochlorothiazide; Benazeprilat; NaOH; SC; rat; 2ML2; 2 weeks; controls received mp w/vehicle; functionality of mp verified by residual volume and plasma assays; dose-response; stability verified by pilot expt; antihypertensive; cardiovascular; agents administered alone and in combination.

P3934 Dryden,S., Pickavance,L., Tidd,D., Williams,G. The lack of specificity of neuropeptide Y (NPY) antisense oligodeoxynucleotides administered intracerebroventricularly in inhibiting food intake and NPY gene expression in the rat hypothalamus. J.Endocrinol. 1998; 157(--):169-175. >>> Oligodeoxynucleotide, antisense; Saline, isotonic; CSF/CNS; rat; 2001; 3, 7 days; controls received mp w/missense oligodeoxynucleotides or saline; stability verified by fluorescence assays; saline infused for 3 days, then pumps replaced with other pumps containing oligodeoxynucleotides to infuse for 7 days.

P3932 Kasamatsu,T., Schmidt,E.K. Continuous and direct infusion of drug solutions in the brain of awake animals: implementation, strengths and pitfalls. Brain Res.Protocols 1997; 1(--):57-69. >>> Forskolin; Dopamine, 6-hydroxy-; Ascorbate; Saline; DMSO; CSF/CNS (visual cortex); cat; 2001; 7 days; no stress (see pg. 64); stability verified by HPLC assays; good methods (extremely detailed for all CSF/CNS aspects and protocols).

P3913 Shibayama,M., Hattori,S., Himes,B.T., Murray,M., Tessler,A. Neurotrophin-3 prevents death of axotomized Clarke's nucleus neurons in adult rat. J.Comp.Neurol. 1998; 390(--):102-111. >>> NT-3; CSF/CNS (spinal cord, intrathecal); rat; 2ML4; 2 months; controls received mp w/PBS; functionality of mp verified by aspirating residual volume; comparison of gel foam vs. mp; long-term study, pumps replaced after 4 weeks; stability verified by bioactivity assay; peptides.

P3880 Cleland,J.L., Barron,L., Berman,P.W., Daugherty,A., Gregory,T., Lim,A., Vennari,J., Wrin,T., Powell,M.F. Development of a single-shot subunit vaccine for HIV-1. 2. Defining optimal autoboost characteristics to maximize the humoral immune response. J.Pharmaceutical Sci. 1996; 85(-12-):1346-1349. >>> GP120; Saponin, QS21; NaCl; Tris; Succinate; SC; guinea-pig; 2002; 2, 4 weeks; comparison of bolus injections vs. microspheres vs. mp; pumps replaced after 2 weeks; stability verified by HPLC, CD4 binding activity and gp120 ELISA; immunology; saponin QS21 used as adjuvant.

P3877 Bartus,R.T., Dean,R.L., Abelleira,S., Charles,V., Kordower,J.H. Dissociation of p75 receptors and nerve growth factor neurotrophic effects: lack of p75 immunoreactivity in striatum following physical trauma, excitotoxicity and NGF administration. Restor.Neurol.Neurosci. 1996; 10(--):49-59. >>> Nerve growth factor; CSF, artificial; BSA; CSF/CNS (striatum); rat; 2002; 14 days; controls received mp w/vehicle; stability verified by in vitro assay; peptides; ALZET brain infusion kit used.

P3860 Zhu,J., Zhang,L., Hanisch,U.K., Felgner,P.L., Reszka,R. A continuous intracerebral gene delivery system for in vivo liposome-mediated gene therapy. Gene Therapy 1996; 3(--):472-476. >>> Liposomes; Gene, herpes simplex virus thymidine kinase; Gene, lacZ; CSF/CNS (caudate nucleus); Rat; 1003D; 3 days; controls received mp w/LacZ gene; tissue perfusion (tumor); functionality of mp verified by gene expression; comparison of intracerebral injections vs. mp; no stress (see pg.473); stability verified by gene expression; ALZET brain infusion kit used; cancer; gene therapy; "DNA-liposome complexes were stable within minipumps at body temperature (37C) for 1-3 days." (pg.474); "continuous administration of DNA-liposome complexes did not result in significant in vivo toxicity." (pg.474).

P3845 Slotkin,T.A., Thai,L., McCook,E.C., Saleh,J.L., Zhang,J., Seidler,F.J. Aging and glucocorticoids: effects on cell signaling mediated through adenylyl cyclase. J.Pharmacol.Exp.Ther. 1996; 279(-2-):478-491. >>> Dexamethasone; Water, bacteriostatic; SC; rat; 2ML4; 26 days; controls received mp w/vehicle; functionality of mp verified by serum levels; stability verified (pg. 1996).

P3809 Mata,J.E., Joshi,S.S., Palen,B., Pirruccello,S.J., Jackson,J.D., Elias,N., Page,T.J., Medlin,K.L., Iversen,P.L. A hexameric phosphorothioate oligonucleotide telomerase inhibitor arrests growth of burkitt's lymphoma cells in vitro and in vivo. Toxicol.Appl.Pharmacol. 1997; 144(--):189-197. >>> Oligodeoxynucleotide, phosphorothioate; PBS; Fluorescein, 5-carboxy; SC; mice (nude); 2002; 7,14 days; controls received mp w/scrambled sequence or saline; no stress (see pg. 194); stability verified by FITC labeled oligo; cancer.

P3780 Redgate,E.S., Grudziak,A.G., Deutsch,M., Boggs,S.S. Difluoromethylornithine enhanced uptake of tritiated putrescine in 9L rat brain tumors. Int.J.Radiat.Oncol.Biol.Phys. 1997; 38(-1-):169-174. >>> Ornithine, difluoromethyl-; Putrescine; CSF, artificial;; IP; CSF/CNS (corpus striatum); rat; 2001; 2002; no duration posted; tissue perfusion (tumor); functionality of mp verified by tumor radioactivity during infusion; stability verified (pg. 173); good methods (pp. 170-171); cancer; catheter used to delay drug infusion for 1 or 3 days after surgery.

P3750 Roy,B.N., Van Vugt,D.A., Weagle,G.E., Pottier,R.H., Reid,R.L. Effect of continuous and multiple doses of 5-aminolevulinic acid on protoporphyrin IX concentrations in the rat uterus. J.Photochem.Photobiol. 1997; 41(--):122-127. >>> Aminolevulinic acid, 5-; Dye, Methylene blue; Saline; intrauterine; rat; 2001D; 12 hours; tissue perfusion (left uterine horn); comparison of intrauterine injections vs. mp; stability verified by uterine PpIX measurements; pH=2 for agent in mp, pH=5-5.5 for injected agent.

P3740 Chen,C., Hanson,S.R., Keefer,L.K., Saavedra,J.E., Davies,K.M., Hutsell,T.C., Hughes,J.D., Ku,D.N., Lumsden,A.B. Boundary layer infusion of nitric oxide reduces early smooth muscle cell proliferation in the endarterectomized canine artery. J.Surg.Res. 1997; 67(--):26-32. >>> Proline, l-; Proline, nitric oxide-; NaOH; IA (femoral); dog; 2ML2; 7 days; controls received mp w/ proline to contralateral vessel; stability verified by in vitro assay; half-life (pg. 27); cardiovascular; constructed local infusion device and attached to mp via catheter.

P3737 Keller,D.A., Marshall,C.E., Lee,K.P. Subchronic nasal toxicity of hexamethylphosphoramide administered to rats orally for 90 days. Fundam.Appl.Toxicol. 1997; 40(--):15-29. >>> Hexamethylphosphoramide; Water; SC; rat; 2ML4; 45, 85 days; comparison of oral dosings vs. mp; long-term study, pumps replaced every 28 days; stability verified by gas chromatography; ALZAID chemical compatibility kit used to test compatibility of agent and vehicle (25% hexamethylphosphoramide); toxicology.

P3717 Yang,D., Kuan,C.-T., Payne,J., Kihara,A., Murray,A., Wang,L.-M., Alimandi,M., Pierce,J.H., Pastan,J.H., Lippman,M.E. Recombinant heregulin-pseudomonas exotoxin fusion proteins: interactions with the heregulin receptors and antitumor activity in vivo. Clin.Cancer Res. 1998; 4(--):993-1004. >>> Heregulin, B1, PE38KDEL; PBS; Albumin, human serum; IP; mice (nude); 1007D; 7 days; dose-response; comparison of IV&intratumoral injections vs. mp; stability verified by in vitro assay; cancer; toxicology; peptides; PE38KDEL is Pseudomonas exotoxin sequence; agent is a recomb. chimeric toxin; mp model was 1007D, not '107'.

P3586 Martinez,F., Deray,G., Dubois,M., Jacquiaud,C., Schmitt,F., Jacobs,C. Chronic effects of endothelin-3 on blood pressure and renal haemodynamics in rats. Nephrol.Dial.Transplant 1996; 11(--):270-274. >>> Endothelin-3; Water, distilled; IV (jugular); rat; 3 days; controls received mp w/ vehicle; stability verified by injection of ET-3 from minipump; stable for 3 days - lost biological activity thereafter; peptides.

P3567 Tsuji-Takayama,K., Harashima,A., Tahata,H., Izumi,N., Nishida,Y., Namba,M., Okuda,Y., Ohta,T., Kurimoto,M. IFN-gamma in combination with IL-3 accelerates platelet recovery in mice with 5-fluorouracil-induced marrow aplasia. J.Interferon and Cytokine Res. 1996; 16(--):447-451. >>> Interferon-gamma; Interleukin-3; Albumin, mouse serum; PBS; SC; mice; 1007D; 7 days; controls received mp w/ vehicle; stability verified after 7 day storage; immunology; peptides; cytokines given singly and together.

P3530 Cardona-Sanclemente,L.E., Born,G.V.R. Increase by adrenaline or angiotensin II of the accumulation of low density lipoprotein and fibrinogen by aortic walls in unrestrained conscious rats. Br.J.Pharmacol. 1996; 117(--):1089-1094. >>> Epinephrine; Angiotensin II; Ascorbic acid; SC; IA (carotid); rat; 2ML4; 1007D; 6 days; controls received saline infusion; functionality of mp verified by plasma levels; stability verified by analyzing residual solution.

P3514 Jones,B.E., Boylan,C.B., Fritsche,M., Juhasz,M., Jackson,C., Wiegand,S.J., Hyman,C., Lindsay,R.M., Alter,C.A. A continuous striatal infusion of 6-hydroxydopamine produces a terminal axotomy and delayed behavioral effects. Brain Research 1996; 709(--):275-284. >>> Dopamine, 6-hydroxy-; Ascorbate; Saline; CSF/CNS (striatum); rat; 2001; 7 days; controls received vehicle infusion; dose-response (figure z); comparison of acute infusion vs. mp; stability verified by bioassay of pump effluent.

P3430 Utley,D.S., Lewin,S.L., Cheng,E.T., Verity,E.T., Sierra,D., Terris,D.J. Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair. Arch Otolaryngol.Head Neck Surg. 1996; 122(--):407-413. >>> Brain-derived neurotrophic factor; Ringer's solution, lactated; CSF/CNS (sciatic nerve); rat; 2ML4; 30 days; functionality of mp verified by measuring residual volume from explanted pumps; comparison of collagen tubule matrix containing BDNF vs. mp; stability verified by bioassay of residual solution; peptides.

P3380 Kinsella,T.J., Glennon,M.C., Kunugi,K.A., Linstrom,M.J. In vivo modulation of iododeoxyuridine metabolism and incorporation into cellular DNA by 5'-amino-5'-deoxythymidine in normal mouse tissues and two human colon cancer xenografts. Clin.Cancer Res. 1996; 2(--):981-989. >>> Uridine, iododeoxy-; Thymidine, 5'-amino-5'-deoxy-; NaOH; SC; mice (nude); 2001; 6 days; controls received mp w/NaOH; 6 day stability verified by uv spectrophotometry p.983; cancer; toxicology; 1-2 mps used per animal; agents given singly&together.

P2779 Ohshima,N., Kotaki,H., Sawada,Y., Iga,T. The relationship between the pharmacological effect of amitriptyline based on an improved forced-swimming test and plasma concentration in rats. Biol.Pharm.Bull 1995; 18(-1-):70-74. >>> Amitriptyline; IP; rat; 2ML1; 7 days; antidepressant; functionality of mp verified by an in vitro study and by assaying plasma levels; stability verified by in vitro studies for 7 days at 37 degrees C.

P2641 Bosker,F.J., van Esseveldt,K.E., Klompmakers,A.A., Westenberg,H.G.M. Chronic treatment with fluvoxamine by osmotic minipumps fails to induce persistent functional changes in central 5-HT1A and 5-HT1B receptors, as measured by in vivo microdialysis in dorsal hippocampus of conscious rats. Psychopharmacology 1995; 117(--):358-363. >>> Fluvoxamine; Saline; SC; rat; 2ML4; 21 days; antidepressant; controls received mp w/ vehicle; functionality of mp verified by plasma levels and residual amount; stability verified at 38 degrees.