References on the Microperfusion of Solid Tissue Using ALZET® Osmotic Pumps

Q0063 Hashish,M., Raghavan,S., Somara,S., Gilmont,R.R., Miyasaka,E., Bitar,K.N., Teitelbaum,D.H. Surgical implantation of a bioengineered internal anal sphincter. Journal of Pediatric Surgery 2010; 45(-1-):52-58. >>> Fibroblast growth factor-2, recomb.; PBS, sterile; SC; Mice; 1004; 25 days; Controls received mp w/ vehicle; tissue perfusion (internal anal sphincter); no stress (see pg. 57); good methods (pg. 53); animal info (female, C57BL/6, 8 wks old); "use of such pumps provided constant infusion over the postimplantation period without observed evidence of systemic effects and allowed increased efficiency of the drug owing to its proximity" pg. 57; operative photographs (pg. 54).

Q0308 Raghavan,S., Miyasaka,E.A., Hashish,M., Somara,S., Gilmont,R.R., Teitelbaum,D.H., Bitar,K.N. Successful implantation of physiologically functional bioengineered mouse internal anal sphincter. American Journal of Physiology-Gastrointestinal and Liver Physiology 2010; 299(-2-):G430-G439. >>> Fibroblast growth factor-2, human, recomb; SC; Mice; 1004; 21 days; Animal info (C57BL/6J female, specific pathogen-free, 8 wk old); ALZET PE catheter used; catheter connected to physiologically functional bioengineered mouse internal anal sphincter; tissue perfusion (internal anal sphincter).

Q0095 Schmidt,N.O., Ziu,M., Cargioli,T., Westphal,M., Giese,A., Black,P.M., Carroll,R.S. Inhibition of thromboxane synthase activity improves glioblastoma response to alkylation chemotherapy. Translational Oncology 2010; 3(-1-):43-49. >>> Furegrelate; PBS; CSF/CNS (intratumoral); Mice (nude); 2004; 4 weeks; Controls received mp w/ vehicle; tissue perfusion (tumor); functionality of mp verified by residual volume; stress/adverse reaction: (see pg. 46) "hemosiderin deposits"; cancer (glioma); ALZET brain infusion kit used; animal info (Swiss, 4-6 wks old).

P9335 Abbaspour,A., Takahashi,M., Sairyo,K., Takata,S., Yukata,K., Inui,A., Yasui,N. Optimal increase in bone mass by continuous local infusion of alendronate during distraction osteogenesis in rabbits. Bone 2009; 44(-5-):917-923. >>> Alendronate; PBS; Bone (tibia); Rabbit; 2ML2; 14 days; Controls received mp w/ vehicle; tissue perfusion (tibia); animal info (male, Japanese White rabbits); disphosphonate; the pump was connected to polyvinyl catheter leading to a hole drilled on the tibia (lengthened segment).

P9944 Agrawal,A., Min,D.H., Singh,N., Zhu,H.H., Birjiniuk,A., von Maltzahn,G., Harris,T.J., Xing,D.Y., Woolfenden,S.D., Sharp,P.A., Charest,A., Bhatia,S. Functional Delivery of siRNA in Mice Using Dendriworms. ACS Nano 2009; 3(-9-):2495-2504. >>> RNA, small interfering; epidermal growth factor receptor; dendriworm, dye-labeled; RNA, small interfering, GFP, dendriworm, dye-labeled; CSF/CNS (intratumoral); Mice; 3, 7 days; Tissue perfusion (tumor); cancer (glioblastoma); incorrectly stated pump model 2007; Plastics One cannula used; animial info (Swiss Webster).

Q0453 Agterberg,M.J.H., Versnel,H., van Dijk,L.M., de Groot,J.C.M.J., Klis,S.F.L. Enhanced Survival of Spiral Ganglion Cells After Cessation of Treatment with Brain-Derived Neurotrophic Factor in Deafened Guinea Pigs. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY 2009; 10(-3-):355-367. >>> Brain-derived neurotrophic factor; BSA; Ear (cochlea); Guinea pig; 2004; 4 weeks; Controls were untreated; animal info (albino, female, 250-350 g); pump connected to Cochlear (R) electrode array; tissue perfusion (cochlea).

P9563 Chakrabarty,S., Shulman,B., Martin,J.H. Activity-Dependent Codevelopment of the Corticospinal System and Target Interneurons in the Cervical Spinal Cord. Journal of Neuroscience 2009; 29(-27-):8816-8827. >>> Muscimol; CSF/CNS (motor cortex); Cat (kitten); 2002; 2 weeks; Controls received mp w/saline; ALZET brain infusion kit used; post op. care (cefazolin, buprenorphine); tissue perfusion (motor cortex).

P9557 Chakrabarty,S., Friel,K.M., Martin,J.H. Activity-Dependent Plasticity Improves M1 Motor Representation and Corticospinal Tract Connectivity. Journal of Neurophysiology 2009; 101(-3-):1283-1293. >>> Muscimol; Saline; CSF/CNS (motor cortex); Cat (kitten); 2002; 28 days; Controls received mp w/vehicle; Long-term study; pumps replaced after 2 weeks; ALZET brain infusion kit used; tissue perfusion (motor cortex).

P9599 Creson,T.K., Hao,Y.L., Engel,S., Shen,Y., Hamidi,A., Zhuo,M., Manji,H.K., Chen,G. The anterior cingulate ERK pathway contributes to regulation of behavioral excitement and hedonic activity. BIPOLAR DISORDERS 2009; 11(-4-):339-350. >>> PD98059; CSF/CNS (anterior cingulate, cortex); Rat; 1002; ; Enzyme inhibitor (ERK); ALZET brain infusion kit 1 used; animal info (male, Sprague Dawley, 250-200g); behavioral testing (open-field, elevated-plus-maze, forced-swim, saccharin preference, amphetamine induced hyperactivity); brain tissue distribution; tissue perfusion (cortex).

P9796 Fransson,A., Jaerlebark,L.E., Ulfendahl,M. In Vivo Infusion of UTP and Uridine to the Deafened Guinea Pig Inner Ear: Effects on Response Thresholds and Neural Survival. Journal of Neuroscience Research 2009; 87(-7-):1712-1717. >>> Uridine triphosphate; uridine; Ear (cochlea); Guinea-pig; 2002; 25 days; Controls received mp w/ artificial perilymph; pumps replaced on day 13; animal info (pigmented, 280-440 g); tissue perfusion.

P9871 Gillio-Meina,C., Phang,S.H., Mather,J.P., Knight,B.S., Kennedy,T.G. Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization. REPRODUCTION 2009; 137(-3-):537-552. >>> Carbaprostacyclin; cicaprost; AFP-07; L-165041; docasahexanoic acid; Ethanol; cyclodextran, beta-; Tris; DMSO; indomethacin; Intrauterine (uterine horn); Rat; 2001; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); good methods (pg. 551); animal info (female, Sprague Dawley, 7 wks old, 200-225 g., OVX); 50% DMSO used; AFP-07 and cicaprost are high-affinity ligands for prostacyclin receptor; carbaprostacyclin is a ligand for both prostacyclin receptor and peroxisome proliferatoractivated receptor delta; tissue perfusion (uterus); 3 mg/ml beta-cyclodextrin used.

P9866 Haylor,J.L., Parker,E., Risbridger,G.P., Beale,D., Brown,B.L., Dobson,P.R.M., Clarke,I.J., Hart,J.E. Inhibition of compensatory renal growth by the N-terminus of a sheep-derived peptide. REGULATORY PEPTIDES 2009; 152(-1-3-):48-53. >>> EPL001; antibody, EPL001, mouse, monoclonal; Kidney; Rat; 2002; ; Tissue perfusion (renal); replacement therapty (unilateral nephrectomy); peptides; animal info (male, Wistar, 250-300 g.); EPL001 is a novel sheep derived 14 amino acid peptide.

P9696 Itoh,T., Satou,T., Nishida,S., Tsubaki,M., Hashimoto,S., Ito,H. Improvement of cerebral function by anti-amyloid precursor protein antibody infusion after traumatic brain injury in rats. MOLECULAR AND CELLULAR BIOCHEMISTRY 2009; 324(-1-2-):191-199. >>> Antibody, anti-amyloid peptide beta; PBS; CSF/CNS (cortex); Rat; 2001; 7 days; Controls received sham operation w/ pump and no cannula; ALZET brain infusion kit 3 used; brain tissue distribution; animal info (male, Wistar, 8-9 wks old, 200-250 g.); traumatic brain injury; Evans blue used to investigate the extent of antibody distribution; behavioral testing (Morris water maze); tissue perfusion (cortex).

P9426 Liu,Y.H., D'Ambrosio,M., Liao,T.D., Peng,H.M., Rhaleb,N.E., Sharma,U., Andre,S., Gabius,H.J., Carretero,O.A. N-acetyl-seryl-aspartyl-lysyl-proline prevents cardiac remodeling and dysfunction induced by galectin-3, a mammalian adhesion/growth-regulatory lectin. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-2-):H404-H412. >>> Galectin-3; Ac-SDKP; Saline; Intrapericardial; Rat; 2004; 2, 4 weeks; Controls received mp w/ vehicle; tissue perfusion (heart); no stress (see pg. H407); cardiovascular; peptides; animal info (male, Sprague Dawley, 275-300 g.); "We used intrapericardial administration of Gal-3 and/or Ac-SDKP in rats. This method allows us to target the heart and obtain site-selective drug efficiency with low-level systemic effects." (p. H404-H405).

P9613 Ma,T., Ortiz,S.G., Huang,Z.N., Ren,P.G., Smith,R.L., Goodman,S.B. In Vivo Murine Model of Continuous Intramedullary Infusion of Particles-A Preliminary Study. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2009; 88B(-1-):250-253. >>> Polystyrene particles, blue dyed; PBS; Bone (femur); Mice; 2004; 4 weeks; Functionality of mp verified by gross inspection of retrieved femora; animal info (9 wks old, male, C57BL/6); Fig. 1, X-ray image of pump and delivery system; tissue perfusion (femur).

Q0333 Nguyen,Y., Couloigner,V., Rudic,M., Grayeli,A.B., Ferrary,E., Sterkers,O. An animal model of cochlear implantation with an intracochlear fluid delivery system. Acta Oto-laryngologica 2009; 129(-11-):1153-1159. >>> Saline; Ear (cochlea); Guinea pig; 1007D; ; Controls received pump without electrode implant; animal info (albino, male, 3 to 7 months old, 290-1030 g.); good methods pg 1154; post op. care (enrofloxacine); "the pump was fixed subcutaneously between the scapulae using a vicryl 3/0 suture (Ethicon)" pg 1154; image of pump-electrode deive, Fig. 1; tissue perfusion (cochlea).

P9844 Orita,H., Shimogori,H., Yamashita,H. Unilateral intra-perilymphatic infusion of substance P enhances ipsilateral vestibulo-ocular reflex gains in the sinusoidal rotation test. Neuroscience Letters 2009; 449(-3-):207-210. >>> Substance P; neurokinin-1 receptor antagonist; Ear (round window); Guinea-pig; 2002; ; Post op. care (piperacillin sodium); animal info (Hartley); pump was connected to a PE catheter filled with artificial perilymph for a 12-hour delayed infusion; tissue perfusion (round window).

P9618 Rousseau,J., Barth,R.F., Moeschberger,M.L., Elleaume,H. EFFICACY OF INTRACEREBRAL DELIVERY OF CARBOPLATIN IN COMBINATION WITH PHOTON IRRADIATION FOR TREATMENT OF F98 GLIOMA-BEARING RATS. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 2009; 73(-2-):530-536. >>> Carboplatin; Dextrose; CSF/CNS (intratumoral); Rat; 2001; 6 days; Controls received mp w/vehicle; no stress (see pg. 532); cancer (glioma); ALZET brain infusion kit 2 used; animal info (male, Fisher, 230-260g); "Surgery for pump implantation was well tolerated." pg. 532; "It is noteworthy that the brainstem was not damaged by direct infusion of 200 ul of carboplatin (0.5 mg/mL) at a flow rate of 1uL/h." pg. 535; tissue perfusion (tumor).

P9478 Schauff,A.K., Kim,E.L., Leppert,J., Nadrowitz,R., Wuestenberg,R., Brockmann,M.A., Giese,A. Inhibition of invasion-associated thromboxane synthase sensitizes experimental gliomas to gamma-radiation. Journal of Neuro-oncology 2009; 91(-3-):241-249. >>> Furegrelate; CSF/CNS (intratumoral); Mice (nude); 1002; 14 days; Controls were untreated; enzyme inhibitor (thromboxane synthase TXSA); cancer (glioma); tissue perfusion (tumor); ALZET brain infusion kit 3 used; animal info (NMRI).

P9515 Scheper,V., Paasche,G., Miller,J.M., Warnecke,A., Berkingali,N., Lenarz,T., Stoever,T. Effects of Delayed Treatment With Combined GDNF and Continuous Electrical Stimulation on Spiral Ganglion Cell Survival in Deafened Guinea Pigs. Journal of Neuroscience Research 2009; 87(-6-):1389-1399. >>> Glial-derived neurotrophic factor; Ear (cochlea); Guinea-pig; 2002; 48 days; Controls received mp w/artificial perilymph; tissue perfusion (cochlea); long-term study; pumps replaced after 13 days; good methods (pg. 1391); animal info (male, pigmented, 250-450 g.); pumps primed; image of pump and electrode cannula device used on fig. 1.

P9872 Unezaki,S., Yoshii,S., Mabuchi,T., Saito,A., Ito,S. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. Journal of Neuroscience Methods 2009; 178(-2-):308-315. >>> Nerve growth factor; glial-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Mice (transgenic); 1004; 4 weeks; Controls received mp w/ vehicle; half-life (p. 308) "short"; animal info (10 wks old, 20 g., Thy1-YEP); image of pump pg. 309; schematic of drug delivery system, fig. 1); "Because of the short biological half-life of neurotrophic factors, a delivery system that protects the protein and slowly releases it locally over a prolonged period of time is required." pg. 308; tissue perfusion (sciatic nerve).

P9833 Yoshida,M., Satoh,T., Nakamura,K.C., Kaneko,T., Hata,Y. Cortical activity regulates corticothalamic synapses in dorsal lateral geniculate nucleus of rats. NEUROSCIENCE RESEARCH 2009; 64(-1-):118-127. >>> Muscimol; Ringer's solution; CSF/CNS (visual cortex); Rat; 1007D; 1002; 2004; 2, 7, 15, 18 days; Controls received mp w/ vehicle; animal info (Long Evans, P21-P49, adult); brain tissue distribution; tissue perfusion (visual cortex).

P8930 Abbaspour,A., Takata,S., Sairyo,K., Katoh,S., Yukata,K., Yasul,N. Continuous local infusion of fibroblast growth factor-2 enhances consolidation of the bone segment lengthened by distraction osteogenesis in rabbit experiment. Bone 2008; 42(-1-):98-106. >>> Fibroblast growth factor-2, recomb. human; Bone (tibia); Rabbit; 2ML4; 14 days; half-life (p. 104) "relatively short"; tissue perfusion (tibia); animal info (male, 1.8-2.2 kg., osteotomized); polyvinyl catheter tubing used; pump image fig. 1e.

P8833 Boodhwani,M., Voisine,P., Rue,M., Sodha,N.R., Feng,J., Xu,S.H., Bianchi,C., Settke,F.W. Comparison of vascular endothelial growth factor and fibroblast growth factor-2 in a swine model of endothelial dysfunction. European Journal of Cardio-thoracic Surgery 2008; 33(-4-):645-650. >>> Vascular endothelial growth factor 165, recomb. human; Heparin; Intramyocardial; Pig (miniswine); 2ML4; 4 weeks; Tissue perfusion (myocardial); half-life (p. 649) "short"; cardiovascular; peptides; ischemia (cardiac); animal info (Yucatan mini-swine, 20-30 kg.).

P9200 Doerr,M.D., Goravanahally,M.P., Rhinehart,J.D., Inskeep,E.K., Flores,J.A. Effects of endothelin receptor type-A and type-B antagonists on prostaglandin F2alpha-induced luteolysis of the sheep corpus luteum. Biology of Reproduction 2008; 78(-4-):688-696. >>> BQ123; BQ610; BQ788; Methanol; saline; Intraovarian (corpus luteum); Sheep; 2002; 14 days; Controls received mp w/ vehicle; tissue perfusion (corpus luteum); good methods (pg. 689); post op. care (penicillin); animal info (female); pumps were primed overnight prior to surgery; vinyl catheter tubing (0007760) used; catheter stabilized to connective tissue capsule and ovarian tunica albuginea using nylon monofilament; all compounds are EDNRA type-A endothelin antagonists.

P9239 Harris,J.A., Iguchi,F., Seidl,A.H., Lurie,D.I., Rubel,E.W. Afferent Deprivation Elicits a Transcriptional Response Associated with Neuronal Survival after a Critical Period in the Mouse Cochlear Nucleus. Journal of Neuroscience 2008; 28(-43-):10990-11002. >>> Tetrodotoxin; Citrate buffer; Ear (round window niche); Mice; 1003D; 24 hours; Controls received mp w/ saline or no treatment to contralateral side; tissue perfusion (round window niche); comparison of cochlear removal vs. mp; animal info (male, female, C57BL/6J, 21 days old).

P9768 Kang,G.S., Di Wang,X., Mohler,M.L., Kirichenko,O.V., Patil,R., Orr,W.E., Miller,D.D., Geisert,E.E. Effects, in an in-vivo model system, of 1,2,3,4-tetrahydroisoquinoline on glioma. Anti-cancer Drugs 2008; 19(-9-):859-870. >>> EDL-155; HBSS; CSF/CNS (intratumoral); Rat; 2001; 7 days; Tissue perfusion (tumor); comparison of IP injections vs. mp; cancer (glioma); animal info (male, Sprague Dawley, 250-350 g); EDL-155 also known as 1-(biphenyl-4-ylmethyl)-1,2,3,4-tetrahydroisoquinoline-6,7-diol.

P9760 Keithley,E.M., Wang,X.B., Barkdull,G.C. Tumor necrosis factor alpha can induce recruitment of inflammatory cells to the cochlea. OTOLOGY&NEUROTOLOGY 2008; 29(-6-):854-859. >>> Tumor necrosis factor-alpha; Ear (cochlea); Guinea-pig; 2001; 2-4 days; Controls received mp w/vehicle; animal info (Hartley albino); tissue perfusion.

P9667 Krum,J.M., Mani,N., Rosenstein,J.M. Roles of the endogenous VEGF receptors flt-1 and flk-1 in astroglial and vascular remodeling after brain injury. Experimental Neurology 2008; 212(-1-):108-117. >>> Antibody, goat, antimouse, flt-1, neutralising; antibody, goat, antimouse, flk-1, neutralizing; immunoglobulin G, goat, normal; CSF, artificial; CSF/CNS (striatum); Rat; 2001; 3, 7 days; Controls received mp w/ goat IgG; animal info (young adult, Wistar); tissue perfusion (striatum).

P9680 Martens,T., Laabs,Y., Guenther,H.S., Kemming,D., Zhu,Z.P., Witte,L., Hagel,C., Westphal,M., Lamszus,K. Inhibition of glioblastoma growth in a highly invasive nude mouse model can be achieved by targeting epidermal growth factor receptor but not vascular endothelial growth factor receptor-2. Clinical Cancer Research 2008; 14(-17-):5447-5458. >>> Cetuximab; CSF/CNS (intratumoral); Mice (nude); 2004; ; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioblastoma); ALZET brain infusion kit 2 used; animal info (NMRI- nu/nu, 6-8 wks old); cetuximab is a monoclonal antibody against EGFR.

P9212 Ortiz,S.G., Ma,T., Regula,D., Smith,R.L., Goodman,S.B. Continuous Intramedullary Polymer Particle Infusion Using a Murine Femoral Explant Model. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2008; 87B(-2-):440-446. >>> Polystyrene; polyethylene, high molecular weight; Serum, mouse; Bone (femur); Mice; 2004; 2, 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by blue-dyed polystyrene particles; tissue perfusion (femur); dose-response (fig. 4); no stress (see pg. 443); animal info (male, C57BL/6, 12-16 wks old); mp connected to hollow titanium rod by vinyl catheter; titanium rod inserted into femur ex vivo; femur/pump assemblies placed in sterile flasks at 37 degree for 2, 4 wks; "There were no cases of infection or malfunction of the pumps apparatus over the course of the experiment." (p. 443).

P9306 Panford-Walsh,R., Singer,W., Ruettiger,L., Hadjab,S., Tan,J., Geisler,H.S., Zimmermann,U., Koepschall,I., Rohbock,K., Vieljans,A., Oestreicher,E., Knipper,M. Midazolam reverses salicylate-induced changes in brain-derived neurotrophic factor and Arg3.1 expression: Implications for tinnitus perception and auditory plasticity. MOLECULAR PHARMACOLOGY 2008; 74(-3-):595-604. >>> Midazolam; Ear (round window niche); Rat; 2001; 7 days; Controls received mp w/ artificial perilymph; comparison of systemic injections vs. mp; animal info (female, Wistar, 200-300 g., cochlear trauma); behavioral testing (tinnitus perception via sound/reward); tissue perfusion (round window niche).

P9121 Qiang,Y.W., Shaughnessy,JD Jr, Yaccoby,S. Wnt3a signaling within bone inhibits multiple myeloma bone disease and tumor growth. Blood 2008; 112(-2-):374-382. >>> Gene, Wnt3a, recomb.; Bone; Mice (SCID); 1004; 4 weeks; Controls received mp w/ PBS; animal info (Myelomatous SCID-hu); tissue perfusion (mylematous bone); Wnt3a is a human gene; ALZET pump was "directly connected to the open side of the implanted bone, allowing continual exposure of the myelomatous bone to rWnt3a".

P9224 Reyes,J.H., O'Shea,K.S., Wys,N.L., Velkey,J.M., Prieskorn,D.M., Wesolowski,K., Miller,J.M., Altschuler,R.A. Glutamatergic Neuronal Differentiation of Mouse Embryonic Stem Cells after Transient Expression of Neurogenin 1 and Treatment with BDNF and GDNF: In Vitro and In Vivo Studies. Journal of Neuroscience 2008; 28(-48-):12622-12631. >>> Doxycycline; brain-derived neurotrophic factor; glial cell line-derived neurotrophic factor; Ear (scala tympani); Guinea-pig; 2002; 27 days; Controls received no treatment to contralateral ear; pumps replaced; peptides; tissue perfusion (scala tympani); animal info (NIH strain, 275-315 g., deafened); cannula and catheter contained doxycycline, mp contained BDNF/GDNF (delayed delivery) to follow, thus providing 2 days Dox, 25 days BDNF/GDNF.

P9218 Shepherd,R.K., Coco,A., Epp,S.B. Neurotrophins and electrical stimulation for protection and repair of spiral ganglion neurons following sensorineural hearing loss. Hearing Research 2008; 242(-1-2-):100-109. >>> Brain-derived neurotrophic factor, recomb. human; Ringer's solution; albumin, guinea pig; Ear (scala tympani); Guinea-pig; 2004; 4 weeks; Controls received no treatment to contralateral cochlea; functionality of mp verified by residual volume and intact connections; peptides; post op. care (Carprofen, Baytril); tissue perfusion (scala tympani); animal info (pigmented, 400-844 g., kanamycin/furosemide deafened).

P9217 Silva,G.B., Garvin,J.L. Angiotensin II-Dependent Hypertension Increases Na Transport-Related Oxygen Consumption by the Thick Ascending Limb. Hypertension 2008; 52(-6-):1091-1098. >>> Angiotensin II; dye, coomassie blue R250; G86976; tempol; SC; kidney (outer medulla); Rat; 1007D; 4, 7 days; Controls received mp w/ vehicle; tissue perfusion (kidney); functionality of mp verified by residual volume and dye infusion; enzyme inhibitor (PKC a/b); cardiovascular; antihypertensive; peptide; cyanoacrylate adhesive; animal info (Sprague Dawley, 7 wks old, 200-250 g.); catheter fenestrated in one side 10 mm from the tip.

P9051 Song,B.N., Li,Y.X., Han,D.M. Effects of delayed brain-derived neurotrophic factor application on cochlear pathology and auditory physiology in rats. Chinese Medical Journal 2008; 121(-13-):1189-1196. >>> Brain-derived neurotrophic factor, recomb. human; Albumin, rat; Ringer's solution; Ear (cochlea); Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (male, albino, Sprague Dawley, 10-12 wks old, 220-250 g.); tissue perfusion (cochlea); functionality of mp verified by residual volume; good methods pg. 1191; pumps were primed in sterile saline at 37 degree Celsius for 48 hours; 1-cm len.

R0266 Swan,E.E.L., Mescher,M.J., Sewell,W.F., Tao,S.L., Borenstein,J.I. Inner ear drug delivery for auditory applications. Advanced Drug Delivery Reviews 2008; 60(-15-):1583-1599. >>> Cisplatin; Sodium thiosulfate; Brain-derived neurotrophic factor; Fibroblast growth factor; D-JNKI-1; BN82270; Tetrodotoxin; Perilymph, artificial; Dexamethasone; Methylprednisone; Caroverine; Methionine, D-; Thiourea; Liposome, cationic; Neomycin; SC; Ear (round window membrane); Ear (cochlea); Ear (scala tympani); Ear; Guinea pig; 3, 7, 14, 28 days; Gene therapy; peptdies; no stress; enzyme inhibitor (peroxidase); stress/adverse reaction (see pg 1593) "Ref #161 found local trauma and inflammatory responses"; tissue perfusion (scala tympani, cochlea, round window membrane); comparison of middle ear injections vs. mp; Review, see pgs. 1587 - 1589, 1591, 1593 - 1595, refs #49, 50, 60, 63, 72, 75, 102, 104,180, 181, 194-201,.

P9292 Vivero,R.J., Joseph,D.E., Angeli,S., He,J., Chen,S.B., Eshraghi,A.A., Balkany,T.J., Van de Water,T.R. Dexamethasone Base Conserves Hearing from Electrode Trauma-Induced Hearing Loss. Laryngoscope 2008; 118(-11-):2028-2035. >>> Dexamethasone base; Perilymph, artificial; Ear (scala tympani); Guinea-pig; 2001; 8 days; Controls received mp w/ vehicle; replacement therapy (cochleostomy); tissue perfusion (scala tympani); animal info (pigmented, 250-300 g.).

P9687 Yatabe,J., Sanada,H., Midorikawa,S., Hashimoto,S., Watanabe,T., Andrews,P.M., Armando,I., Wang,X.Y., Felder,R.A., Jose,P.A. Effects of Decreased Renal Cortical Expression of G Protein-Coupled Receptor Kinase 4 and Angiotensin Type 1 Receptors in Rats. HYPERTENSION RESEARCH 2008; 31(-7-):1455-1464. >>> Oligodeoxynucleotide, antisense; oligodeoxynucleotide, scrambled; G-protein-coupled receptor kinase 4; Ringer's solution, lactated; Kidney; Rat; 5 weeks; Controls received mp w/ vehicle; pumps replaced following 1 week recovery period; antisense (GRK4, AT1R); animal info (4 wks old, male, WKY, SHR, uninephrectomized); tissue perfusion (kidney).

P9206 Zagorac,D., Jakovcevic,D., Gebremedhin,D., Harder,D.R. Antiangiogenic effect of inhibitors of cytochrome P450 on rats with glioblastoma multiforme. Journal of Cerebral Blood Flow and Metabolism 2008; 28(-8-):1431-1439. >>> Miconazole; octadecynoic acid, 17-; Ethanol; CSF, artificial; CSF/CNS (intratumoral); Rat; 1002; 14 days; Controls received mp w/ vehicle; enzyme inhibitor (CYP epoxygenase); tissue perfusion (tumor); ALZET brain infusion kit used; animal info (male, Fisher, 8-10 wks old); 10% ethanol used; antiangiogenesis.

P8366 Dorrell,M.I., Aguilar,E., Scheppke,L., Barnett,F.H., Friedlander,M. Combination angiostatic therapy completely inhibits ocular and tumor angiogenesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-3-):967-972. >>> Vascular endothelial growth factor, aptamer; EMD472523; T2-TrpRS; PBS; CSF/CNS (intratumoral); Rat; 2001D; 2ML2; 2ML4; 3,6 days; 24 hours; Controls received mp w/ vehicle; cancer (gliosarcoma); animal info (male, Fischer, 344); EMD472523 is an integrin antagonist, VEGF aptamer is chemically identical to Macugen; T2 is a proteolytic fragment of tryptophan tRNA synthetase; all agents have angiostatic activity; tissue perfusion (tumor).

P7978 Engelhardt,M., Di Cristo,G., Berardi,N., Maffei,L., Wahle,P. Differential effects of NT-4, NGF and BDNF on development of neurochemical architecture and cell size regulation in rat visual cortex during the critical period. European Journal of Neuroscience 2007; 25(-2-):529-540. >>> Nerve growth factor, mouse; NT-4, human recomb.; brain-derived neurotrophic factor, recomb. human; CSF/CNS (visual cortex); Rat; 1007D; 8 days; Post op. care (antibiotics and local anesthetics); animal info (Long-Evans); dental cement used; 30 G cannula used; tissue perfusion (visual cortex).

P8429 Frederiksen,B.L., Caye-Thomasen,P., Lund,S.P., Wagner,N., Asal,K., Olsen,N.V., Thomsen,J. Does erythropoietin augment noise induced hearing loss?. Hearing Research 2007; 223(-1-2-):129-137. >>> Erythropoietin; Ear (round window); Guinea-Pig; 1007D; 1 week; Controls received mp w/ saline; replacement therapy (noise-induced hearing impairment); comparison of acute admin. vs. mp; peptides; animal info (male, Dunkin-Hartley); tissue perfusion (round window); mp primed 6 hours in 37 Celsius saline; correct catheter placement confirmed.

P9777 Giraud,S., Bessette,B., Boda,C., Lalloue,F., Petit,D., Mathonnet,M., Jauberteau,M.O. In vitro apoptotic induction of human glioblastoma cells by Fas ligand plus etoposide and in vivo antitumour activity of combined drugs in xenografted nude rats. INTERNATIONAL JOURNAL OF ONCOLOGY 2007; 30(--):273-281. >>> Fas ligand; etoposide; dexamethasone; CSF, artificial; CSF/CNS (intratumoral); Rat (nude); 1002; 15 days; Tissue perfusion (tumor); functionality of mp verified by residual volume; cancer (glioblastoma); animal info (female 2 months old, 159 g.).

P8132 Haruta,M., Hata,Y. Experience-driven axon retraction without binocular imbalance in developing visual cortex. CURRENT BIOLOGY 2007; 17(-1-):37-42. >>> Muscimol; Saline; CSF/CNS (visual cortex, area 17); Cat (kitten); 2002; 2 weeks; Brain tissue distribution; post op. care (enrofloxacin); animal info (4 wks old, vision deprivation by eyelid suture); tissue perfusion (visual cortex).

P8878 Hontanilla,B., Auba,C., Gorria,O. Nerve regeneration through nerve autografts after local administration of brain-derived neurotrophic factor with osmotic pumps. Neurosurgery 2007; 61(-6-):1268-1274. >>> Brain-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Rat; 1002; 2 weeks; Controls received mp w/ vehicle; tissue perfusion (sciatic nerve graft); replacement therapy (axotomy); no stress (see pg. 1270); stress/adverse reaction: (see pg. 1270) "one pump was extruded"; animal info (male, Wistar, 200-250g.); pg. 1269, fig A shows image of pump implantation and cannulae location.

P8724 Hori,R., Nakagawa,T., Sakamoto,T., Matsuoka,Y., Takebayashi,S., Ito,J. Pharmacological inhibition of Notch signaling in the mature guinea pig cochlea. NeuroReport 2007; 18(-18-):1911-1914. >>> MDL 28170; DMSO; PBS; Ear (cochlea); Guinea-pig; 1002; 14 days; Tissue perfusion (cochlea); enzyme inhibitor (gamma secretase); peptides; animal info (Hartley strain, 350-400g); MDL 28170 is a gamma-secretase inhibitor; 0.3% DMSO used; "a silicon tube (180 micron OD) connected to a micro-osmotic minipump...".

P8680 Joyce,M.M., Burghardt,R.C., Geisert,R.D., Burghardt,J.R., Hooper,R.N., Ross,J.W., Ashworth,M.D., Johnson,G.A. Pig conceptuses secrete estrogen and interferons to differentially regulate uterine STAT1 in a temporal and cell type-specific manner. Endocrinology 2007; 148(-9-):4420-4431. >>> Conceptus secretory proteins, porcine; Intrauterine; Pig; 2ML1; ; Controls received mp w/ albumin, porcine serum; tissue perfusion (uterus); good methods p. 4421; peptides; multiple pumps per animal (2); animal info (female).

P8536 Joyce,M.M., Burghardt,J.R., Burghardt,R.C., Hooper,R.N., Jaeger,L.A., Spencer,T.E., Bazer,F.W., Johnson,G.A. Pig conceptuses increase uterine interferon-regulatory factor 1 (IRF1), but restrict expression to stroma through estrogen- induced IRF2 in luminal epithelium. Biology of Reproduction 2007; 77(-2-):292-302. >>> Conceptus secretory proteins, porcine; Albumin, porcine serum; dye, india ink; PBS; Intrauterine (uterine horn); Pig; 2ML1; 4 days; Controls received mp w/ PSA; peptides; multiple pumps per animal (2); animal info (female, pseudopregnant); india ink used to confirm agent distribution in uterus; tissue perfusion (uterine horn).

P8149 Kovacs,P., Morales,J.C., Karkanias,G.B. The effect of diabetes and centrally administered insulin on anterior hypothalamic estrogen receptor alpha immunoreactivity. ACTA DIABETOLOGICA 2007; 44(-1-):38-44. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (STZ-induced Diabetes, ovariectomy); peptides; animal info (female, Sprague-Dawley, 175-200g.); tissue perfusion (ventricle).

P8281 Lee,M.C., Bier,A.D., Nickisch,F., Eberson,C.P., Ehrlich,M.G., Chen,Q. Epiphysiodesis with infusion of stromal cell-derived factor-1 in rabbit growth plates. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME 2007; 89A(-1-):102-113. >>> Stromal cell-derived factor-1a, recomb. human; dye, methylene blue; PBS; hypaque; Bone (tibial physis); Rabbit; 2001D; 2004; 2,4 weeks; 24 hours; Controls received mp w/ vehicle and control leg w/ no treatment; functionality of mp verified by methylene blue study, hypaque; good methods (p. 103-105); peptides; post op. care (buprenorphine); tissue perfusion (tibia physis); animal info (New Zealand white, 6 weeks old, 2.5 kg); catheter with 16 fenestrations used; 30% hypaque, which "served as a radio-opaque marker of pump reservoir level."; "A continuous, implantable, direct delivery system offers a practical model for studying the local effects of various reagents on the physis and a therapeutic model by which agents for use in epiphysiodesis may be evaluated." (p. 110).

P8252 Lee,Y., Gaskins,D., Anand,A., Shekhar,A. Glia mechanisms in mood regulation: a novel model of mood disorders. Psychopharmacology 2007; 191(-1-):55-65. >>> PDC; CSF, artificial; CSF/CNS (basolateral amygdala); Rat; 1002; 4, 7, 8 days; Controls received mp w/ vehicle; dose-response (fig. 1); animal info (male, Wistar, 275-300g.); L-trans-pyrrolidine-2, 4-dicarboxylic acid, a glial&neuronal glutamate transport inhibitor; cannulae location verified with cresyl violet staining; mood disorder model; tissue perfusion (basolateral amygadala).

P8478 Ma,T., Gutnick,J., Salazar,B., Larsen,M.D., Suenaga,E., Zilber,S., Huang,Z., Huddleston,J., Smith,R.L., Goodman,S. Modulation of allograft incorporation by growth factors over a prolonged continuous infusion of duration in vivo. Bone 2007; 41(-3-):386-392. >>> Fibroblast growth factor-2; osteogenic protein-1, recomb. human; Water, distilled; acetate buffer; Bone (tibia); Rabbit; 2004; 4,36 weeks; Controls received no treatment to contralateral side; long-term study; pumps replaced every 4 weeks, with 4 week rest periods between 4 week infusion periods; animal info (New Zealand white, 6-12 months old, 3.5-4.2 kg); peptides; OP-1 also known as bone morphogenic protein-7 (BMP-7); tissue perfusion (tibia); unique drug test chamber and bone harvest chamber used in conjunction with mp.

P8402 Maruyama,J., Yamagata,T., Ulfendahl,M., Bredberg,G., Altschuler,R.A., Miller,J.M. Effects of antioxidants on auditory nerve function and survival in deafened guinea pigs. NEUROBIOLOGY OF DISEASE 2007; 25(-2-):309-318. >>> Trolox; neomycin; ascorbic acid; Perilymph, artificial; sodium bicarbonate; Ear (cochlea); Guinea-Pig; 2002; 26 days; Controls received mp w/ vehicle; pumps replaced after 14 days; post op. care (doxycycline); animal info (male, pigmented, 250-400g., neomycin deafening); cannula primed with 10% neomycin solution followed by a small air bubble spacer to allow neomycin infusion for first 2 days; trolox, a vitamin F analogue, and ascorbic acid delivered together in 1 mp; tissue perfusion (cochlea).

P8729 Masunaga,S., Takahashi,A., Ohnishi,K., Nagata,K., Suzuki,M., Kashino,G., Kinashi,Y., Ohnishi,T., Ono,K. Dependency of repair of radiation-induced damage on p53 status and efficient inhibition of the repair with wortmannin treatment in vivo, with reference to the effect on intratumor quiescent cell population. Oncology Reports 2007; 18(-6-):1589-1596. >>> Uridine, bromodeoxy-; Saline; SC; Mice (nude); 2001; 7 days; Tissue perfusion (tumor); cancer; animal info (Balb/cA, nude); labeling of tumor cells.

P8661 Miller,J.M., Le Prell,C.G., Prieskorn,D.M., Wys,N.L., Altschuler,R.A. Delayed neurotrophin treatment following deafness rescues spiral ganglion cells from death and promotes regrowth of auditory nerve peripheral processes: Effects of brain-derived neurotrophic factor and fibroblast growth factor. Journal of Neuroscience Research 2007; 85(-9-):1959-1969. >>> Brain-derived neurotrophic factor; fibroblast growth factor-1; Perilymph, artificial; albumin, guinea pig serum; Ear (scala tympani); Guinea-pig; 2002; 26 days; Controls received mp w/ vehicle; pumps replaced at day 13; peptides; tissue perfusion (scala tympani); animal info (male, female, pigmented, 250-300g, deafened).

P8738 Roehm,P., Hoffer,M., Balaban,C.D. Gentamicin uptake in the chinchilla inner ear. Hearing Research 2007; 230(-1-2-):43-52. >>> Gentamicin; Ear (round window); Chinchilla; 2002; 1, 3, 6, 14 days; 4, 8 hours; Comparison of transtympanic injections vs. mp; tissue perfusion (round window); animal info (male, female, chinchilla langier).

P8676 Royo,N.C., LeBold,D., Magge,S.N., Chen,I., Hauspurg,A., Cohen,A.S., Watson,D.J. Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function. Neuroscience 2007; 148(-2-):359-370. >>> NT-4/5, recomb. human; PBS; BSA; CSF/CNS (somatosensory cortex); 1007D; ; Controls received mp w/ vehicle; tissue perfusion (cortex); good methods (p. 360); ALZET brain infusion kit 2 used; brain tissue distribution; post op. care (recovery on heating pad); animal info (male, Sprague-Dawley, 350-400g); 0.1% BSA; traumatic brain injury.

P9024 Skoglund,B., Aspenberg,P. Locally applied Simvastatin improves fracture healing in mice. BMC MUSCULOSKELETAL DISORDERS 2007; 8(-;-):U1-U6. >>> Simvastatin; PEG 400; SC; bone (femur); Mice; 1002; 14 days; Comparison of SC injections vs. SC mp vs. localized delivery; half-life (p. 2 of 6) "about 2 hours in humans"; animal info (mature, male, Balb-C); tissue perfusion (femur); compound passed through sterile filter before filling in pumps; silcone tube used; "continuous systemic delivery resulted in a 160% larger force at failure."; "Continuous local delivery... resulted in a 170% larger force at failure as well as a twofold larger energy uptake.".

P8496 Sun,Z.Y., Rafferty,K.L., Egbert,M.A., Herring,S.W. Masticatory mechanics of a mandibular distraction osteogenesis site: Interfragmentary micromovement. Bone 2007; 41(-2-):188-196. >>> Amikacin; Sub-masseteric tunnel; Pig; 2ML2; 14 days; Bone healing; x-ray image showing pump adjacent to mandible bone (p. 189); pump positioned near mandible bone to deliver antibiotics for infection control; tissue perfusion (sub-masseteric tunnel).

P8368 Wang,J., Ruel,J., Ladrech,S., Bonny,C., Van de Water,T.R., Puel,J.L. Inhibition of the c-Jun N-terminal kinase-mediated mitochondrial cell death pathway restores auditory function in sound-exposed animals. MOLECULAR PHARMACOLOGY 2007; 71(-3-):654-666. >>> Jun, c-, N-Terminal kinase Inhibitor-1, D-; peptide, D-TAT; Jun, c-, N-Terminal kinase Inhibitor-1-mutant; Perilymph, artificial; PBS; Ear (round window membrane); Guinea-Pig; 2001; 7 days; Controls received mp w/ vehicle, or inactive JNKI-1-mutant or TAT-empt peptide or contralateral untreated ear; dose-response (fig 6); comparison of acute infusion vs. hyaluronic acid gel vs.; enzyme inhibitor (c-Jun N-terminal kinase); peptides; animal info (pigmented, 250-300g.; sound trauma); D-JNKI-1 peptide contains a 10-amino acid HIV-TAT transporter sequece to facilitate its entry into cells; tissue perfusion (round window membrane).

P7968 Wang,Y.L., Wang,D.Z., Nie,X., Lei,D.L., Liu,Y.P., Zhang,Y.J., Suwa,F., Tamada,Y., Fang,Y.R., Jin,Y. The role of bone morphogenetic protein-2 in vivo in regeneration of peripheral nerves. BRITISH JOURNAL OF ORAL&MAXILLOFACIAL SURGERY 2007; 45(-3-):197-202. >>> Bone morphogenetic protein-2; Saline; CSF/CNS (facial nerve); Rabbit; 2ML4; 10 days; Controls received mp w/ vehicle; animal info (New Zealand, 1.6-2.3 kg); total daily infused volume incorrect; tissue perfusion (facial nerve).

P9142 Wazen,R.M., Tye,C.E., Goldberg,H.A., Hunter,G.K., Smith,C.E., Nanci,A. In vivo functional analysis of polyglutamic acid domains in recombinant bone sialoprotein. JOURNAL OF HISTOCHEMISTRY&CYTOCHEMISTRY 2007; 55(-1-):35-42. >>> Bone sialoprotein, recomb., rat, -DNP; bone sialoprotein, recomb., rat, -DNP, mutated; Bone (hemimandible); Rat; 1003D; 2001D; 1, 3 days; Negative controls received mp w/ sodium chloride or were sham operated; peptides; tissue perfusion (right hemimandible); post op. care (buprenorphine hydrochloride); animal info (Wistar, 100g.); bone sialoprotein (BSP) is an anionic phosphoprotein; prBSP-DNP's are prokaryotic recombinant BSP's tagged with dinitrophenol.

P8152 Abizaid,A., Liu,Z.W., Andrews,Z.B., Shanabrough,M., Borok,E., Elsworth,J.D., Roth,R.H., Sleeman,M.W., Picciotto,M.R., Tschoep,M.H., Gao,X.B., Horvath,T.L. Ghrelin modulates the activity and synaptic input organization of midbrain dopamine neurons while promoting appetite. Journal of Clinical Investigation 2006; 116(-12-):3229-3239. >>> BIM-28163; Saline; CSF/CNS (ventral tegmental area); Mice; 1002; 7 days; Controls received mp w/ vehicle; animal info (C57BL/6); cannula placement confirmed by histological exam w/ cresyl violet; tissue perfusion (ventral tegmental area).

P7938 Bartlett,C.S., Rapuano,B.E., Lorich,D.G., Wu,T., Anderson,R.C., Tomin,E., Hsu,J.F., Lane,J.M., Helfet,D.L. Early changes in prostaglandins precede bone formation in a rabbit model of heterotopic ossification. Bone 2006; 38(-3-):322-332. >>> AH 6809; cyclic AMP, Rp-; IM (vastus intermedius); Rabbit; 2ML2; 14 days; Functionality of mp verified by residual volume; no stress (see pg. 326); animal info (New Zealand); plain and high resolution (Faxitron) radiographs; fig 7 and 9; tissue perfusion (vastus intermedius); agent is a PGE2/PGD receptor antagonist.

P7937 Bartoli,M.A., Parodi,F.E., Chu,J., Pagano,M.B., Mao,D.L., Baxter,B.T., Buckley,C., Ennis,T.L., Thompson,R.W. Localized administration of doxycycline suppresses aortic dilatation in an experimental mouse model of abdominal aortic aneurysm. Annals of Vascular Surgery 2006; 20(-2-):228-236. >>> Doxycycline hydrochloride; Saline; SC; IP; aorta; Mice; 1002; 14 days; Controls received mp w/ vehicle; tissue perfusion (aorta); dose-response (table 1); comparison of oral vs. mp; half-life (p. 234), 26.5 hours; cardiovascular; animal info (C57BL/6J, male, 8-10 weeks old, 20-35 grams); catheter secured to a polyvinyl alcohol sponge positioned over the anterior surface of the aorta and secured to retroperitoneal tissues; (quote p. 233).

P8053 Bruno,M.A., Cuello,A.C. Activity-dependent release of precursor nerve growth factor, conversion to mature nerve growth factor, and its degradation by a protease cascade. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006; 103(-17-):6735-6740. >>> Neuroserpin; GM6001; CSF, artificial, PBS; CSF/CNS (parietal cortex); Rat; 2001; 72 hours; Controls received mp w/ vehicle into contralateral side; enzyme inhibitor (tpa, metalloproteinase); multiple pumps per animal (2); animal info (Fischer 344, 6 month old); Lynch coil with air-oil spacer at mp end of tubing; bilateral infusion cannulae; tissue perfusion (parietal cortex).

P8081 de Yebra,L., Malpesa,Y., Ursu,G., Pugliese,M., Lievens,J.C., Goff,L.K.L., Mahy,N. Dissociation between hippocampal neuronal loss, astroglial and microglial reactivity after pharmacologically induced reverse glutamate transport. NEUROCHEMISTRY INTERNATIONAL 2006; 49(-7-):691-697. >>> PDC; CSF/CNS (hippocampus); Rat; 1007D; 14 days; Controls received mp w/ PBS; dose-response (fig.2); animal info (male, Wistar, 220-240g.); L-trans-pyrrolidine-2, 4-dicarboxylate; tissue perfusion (hippocampus).

P9506 Fowlkes,JL  Journal of Bone and Mineral Research 2006; 21(-9-):1359-1366. >>> Insulin-like growth factor I, recomb. human; dye, methylene blue; PBS; SC; bone (tibia); bone (fibula); Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by rhIGF-I serum levels; replacement therapy (mid-diaphyseal osteotomy (tibia, fibula)); peptides; post op. care (buprenex); tissue perfusion (tibia, fibula); animal info (male, C57BL/6, 18 months old).

P8062 Gelling,R.W., Morton,G.J., Morrison,C.D., Niswender,K.D., Myers,MG Jr, Rhodes,C.J., Schwartz,M.W. Insulin action in the brain contributes to glucose lowering during insulin treatment of diabetes. Cell Metabolism 2006; 3(-1-):67-73. >>> LY294002; CSF/CNS (third ventricle); Rat; 2002; 9 days; Controls received mp w/ vehicle; comparison of icv injections vs. mp; enzyme inhibitor (phosphatidylinositol 3-kinase); animal info (male, Sprague-Dawley, 250-350g); cannula placement confirmed; tissue perfusion (ventricle).

P8145 Grommes,C., Landreth,G.E., Sastre,M., Beck,M., Feinstein,D.L., Jacobs,A.H., Schlegel,U., Heneka,M.T. Inhibition of in vivo glioma growth and invasion by peroxisome proliferator-activated receptor gamma agonist treatment. MOLECULAR PHARMACOLOGY 2006; 70(-5-):1524-1533. >>> Pioglitazone; DMSO; PBS; CSF/CNS (intratumoral); Rat; 2ML4; 3, 6, 9, 14, 21 days; Controls received mp w/ vehicle; comparison of oral admin. vs. mp; no stress (see p.1532); stability verified by in vitro incubation at 37 celsius for 21 days; cancer (glioma); ALZET brain infusion kit used; animal info (Sprague-Dawley, 200-250g.); 0.1% DMSO; Antineoplastic; tissue perfusion (tumor).

P8129 Heinrich,C., Nitta,N., Flubacher,A., Muller,M., Fahrner,A., Kirsch,M., Freiman,T., Suzuki,F., Depaulis,A., Frotscher,M., Haas,C.A. Reelin deficiency and displacement of mature neurons, but not neurogenesis, underlie the formation of granule cell dispersion in the epileptic hippocampus. Journal of Neuroscience 2006; 26(-17-):4701-4713. >>> Antibody, monoclonal, CR-50; immunoglobulin G, mouse; DMEM; CSF/CNS (hippocampus); Mice; 1007D; 7 days; Controls received mp w/ mouse IgG; ALZET brain infusion kit use; brain tissue distribution; animal info (male, C57BL/6, 8-10 wks old); Reelin-neutralizing antibody; DMEM is Dulbecco's modified eagle medium; tissue perfusion (hippocampus).

P8078 Hoe,H.S., Pociuausek,A., Dai,H.N., Chakraborty,G., Harris,D.C., Rebeck,G.W. Effects of apoE on neuronal signaling and APP processing in rodent brain. Brain Research 2006; 1112(-;-):70-79. >>> Apolipoprotein E; apolipoprotein E3, recomb human; apolipoprotein E4, recomb human; Saline; CSF/CNS (dorsal hippocampus); Rat; 2001; 1 week; Controls received mp w/ vehicle; comparison of icv injections vs. mp; peptides; animal info (Sprague-Dawley, 230-250g.); correct infusion sites confirmed post mortem; tissue perfusion (hippocampus).

P8141 Huang,Q.L., Timofeeva,E., Richard,D. Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. Journal of Endocrinology 2006; 191(-1-):179-188. >>> Leptin, recomb. murine; CSF/CNS (third ventricle); Rat; 2001; 5 days; Controls received mp w/ PBS; peptides; animal info (male, Wistar, 270-290g.); tissue perfusion (ventricle).

P8017 Kawai,T., Takagi,N., Mochizuki,N., Besshoh,S., Sakanishi,K., Nakahara,M., Takeo,S. Inhibitor of vascular endothelial growth factor receptor tyrosine kinase attenuates cellular proliferation and differentiation to mature neurons in the hippocampal dentate gyrus after transient forebrain ischemia in the adult rat. Neuroscience 2006; 141(-3-):1209-1216. >>> Vascular endothelial growth factor receptor tyrosine kinase inhibitor; DMSO; CSF/CNS (dentate gyrus); Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig. 4); enzyme inhibitor (VEGF receptor tyrosine kinase); ischemia (transient forebrain); animal info (male, Wistar, 8 weeks old, carotid artery occlusion); 0.5% DMSO; agent also known as 4-[(4'-chloro-2'-fluoro) phenylamino]-6,7-dimethoxyquinazoline; tissue perfusion (dentate gyrus).

P8986 Li,Y., Wo,J.M., Ellis,S., Ray,M.B., Jones,W., Martin,R.C.G. Morphological transformation in esophageal submucosa by bone marrow cells: Esophageal implantation under external esophageal perfusion. STEM CELLS AND DEVELOPMENT 2006; 15(-5-):697-705. >>> Bile, bovine; HCl; Intraluminal (esophagus); Rat; 2ML1; 4 weeks; Controls received mp w/ saline; tissue perfusion (esophagus); pumps replaced every 7 days; animal info (male, Sprague Dawley, 8 wks old); hand-stretched, PE-50 catheter; esophagitis model; gastroenterology; good methods.

P8020 Ma,T., Nelson,E.R., Mawatari,T., Oh,K.J., Larsen,D.M., Smith,R.L., Goodman,S.B. Effects of local infusion of OP-1 on particle-induced and NSAID-induced inhibition of bone ingrowth in vivo. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 2006; 79A(-3-):740-746. >>> OP-1, recomb. human; Acetate buffer; Bone (tibia); Rabbit; 2004; 6, 24 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced every 4-6 weeks; stress/adverse reaction (see pg. 742) implant (DTC and mp) infection in one animal [manufacturer note: pumps are provided sterile]; peptides; tissue perfusion (tibia); post op. care (cephalosporin); animal info (male, New Zealand white, 6-12 months old, 3.5-4.2 kg); agent also know as bone morphogenic protein-7; mp implanted with a titanium drug test chamber (DTC).

P8208 Martens,T., Schmidt,N.O., Eckerich,C., Fillbrandt,R., Merchant,M., Schwall,R., Westphal,M., Lamszus,K. A novel one-armed anti-c-met antibody inhibits glioblastoma growth in vivo. Clinical Cancer Research 2006; 12(-20-):6144-6152. >>> Antibody, anti-c-Met, OA5D5; Histidine; CSF/CNS (intratumoral); Mice (nude); 2004; 12, 21, 28 days; Controls received mp w/ vehicle; cancer (glioblastoma); ALZET brain infusion kit 2 used; animal info (male, NMRI-nu/nu, 6-8 wks old); "Because the OA-5D5 antibody is a relatively large but stable molecule, convection-enhanced drug delivery (by mp) was ideally suited to deliver it in our orthotopic model." (p.6151); tissue perfusion (tumor).

P9057 Masunaga,S., Sakurai,Y., Nagata,K., Suzuki,M., Maruhashi,A., Kinashi,Y., Nagasawa,H., Uto,Y., Hori,H., Ono,K. The usefulness of a continuous administration of tirapazamine combined with reduced dose-rate irradiation using gamma-rays or reactor thermal neutrons. British Journal of Radiology 2006; 79(-948-):991-998. >>> Uridine, bromodeoxy-; tirapazamine; Saline; SC; Mice; 2001D; 5 days; 24 hours; Tissue perfusion (tumor); comparison of IP injections vs. mp; cancer; animal info (8-11 wks old, female, syngenic C3H, He); tirapazamine, also known as TP2, is a lead compound as a bioreactive hypoxic cytotoxin; labing of P cells; cancer.

P8195 Mccoy,M.K., Martinez,T.N., Ruhn,K.A., Szymkowski,D.E., Smith,C.G., Botterman,B.R., Tansey,K.E., Tansey,M.G. Blocking soluble tumor necrosis factor signaling with dominant-negative tumor necrosis factor inhibitor attenuates loss of dopaminergic neurons in models of Parkinson's disease. Journal of Neuroscience 2006; 26(-37-):9365-9375. >>> Endotoxin, LPS; XENP345; Saline, sterile; CSF/CNS (striatum); Rat; 2002; 2 weeks; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 200-225 grams, male, CDF, 200-240 grams); neurodegenerative (Parkinson's disease); dominant-negative TNF inhibitor; tissue perfusion (striatum).

P8071 Morrey,J.D., Siddharthan,V., Olsen,A.L., Roper,G.Y., Wang,H., Baldwin,T.J., Koenig,S., Johnson,S., Nordstrom,J.L., Diamond,M.S. Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters. Journal of Infectious Diseases 2006; 194(-9-):1300-1308. >>> Antibody, monoclonal, hE16; CSF, artificial; CSF/CNS (parenchyma); Hamster; 2001D; 24 hours; Controls received mp w/ vehicle, or saline; comparison of ip injections vs. mp; brain tissue distribution; animal info (female, Syrian golden, 7 wks. old, 116g., infected w/ West Nile Virus); hE16 is specific for WNV envelope protein domain III "this delivery system facilitated broad delivery of Ab into the brain, with highest levels near the site of cannulation." (p.1305); tissue perfusion (parenchyma).

P8008 Niimura,M., Takagi,N., Takagi,K., Mizutani,R., Tanonaka,K., Funakoshi,H., Matsumoto,K., Nakamura,T., Takeo,S. The protective effect of hepatocyte growth factor against cell death in the hippocampus after transient forebrain ischemia is related to the improvement of apurinic/apyrimidinic endonuclease/redox factor-1 level and inhibition of NADPH oxidase activity. Neuroscience Letters 2006; 407(-2-):136-140. >>> Hepatocyte growth factor; CSF/CNS (right hippocampal region); Rat; 1003D; 3 days; Animal info (male, Wistar, 200-250 grams); ischemia (cerebral); tissue perfusion (hippocampal region).

P8009 Niimura,M., Takagi,N., Takagi,K., Mizutani,R., Ishihara,N., Matsumoto,K., Funakoshi,H., Nakamura,T., Takeo,S. Prevention of apoptosis-inducing factor translocation is a possible mechanism for protective effects of hepatocyte growth factor against neuronal cell death in the hippocampus after transient forebrain ischemia. Journal of Cerebral Blood Flow and Metabolism 2006; 26(-11-):1354-1365. >>> Hepatocyte growth factor; Saline; CSF/CNS (right hippocampal region); Rat; 1003D; 3 days; Animal info (male, Wistar, 200-250 grams); ischemia (cerebral); tissue perfusion (hippocampal region).

P8176 Radeloff,A., Smolders,J.W.T. Brain-derived neurotrophic factor treatment does not improve functional recovery after hair cell regeneration in the pigeon. Acta Oto-laryngologica 2006; 126(-5-):452-459. >>> Brain-derived neurotrophic factor, recomb. human; PBS; albumin, chicken; Hank's solution; Ear (scala tympani); Bird (pigeon); 2002; 8 weeks; Controls received mp w/ vehicle or no treatment to contralateral ear; long-term study; pumps replaced every 14 days; ALZET brain infusion kit used; peptides; animal info (Columba livia, 6 months old, 320-580 grams); deafening of both ears; tissue perfusion (scala tympani).

P7849 Sanada,H., Yatabe,J., Midorikawa,S., Katoh,T., Hashimoto,S., Watanabe,T., Xu,J., Luo,Y.J., Wang,X.Y., Zeng,C.Y., Armando,I., Felder,R.A., Jose,P.A. Amelioration of genetic hypertension by suppression of renal G protein-coupled receptor kinase type 4 expression. Hypertension 2006; 47(-6-):1131-1139. >>> Oligodeoxynucleotide, phosphorothioate antisense; oligodeoxynucleotide, missense; Ringer's solution, lactated; Kidney; Rat; 2001; 2004; 5 weeks; Controls received mp w/ vehicle, or missense oligos; tissue perfusion (kidney); pumps replaced after 1 week of vehicle; no stress (see pg. 1135); antisense (GRK4); cardiovascular; animal info (male, Wistar-Kyoto, SHR, 4 wk. old).

P8388 Sendowski,I., Abaamrane,L., Raffin,F., Cros,A., Clarencon,D. Therapeutic efficacy of intra-cochlear administration of methylprednisolone after acoustic trauma caused by gunshot noise in guinea pigs. Hearing Research 2006; 221(-1-2-):119-127. >>> Methylprednisolone; perilymph, artificial; Perilymph, artificial; Ear (scala tympani); Guinea-Pig; 2001; 14 days; Controls received mp w/ vehicle, or non-implanted ear; dose-response (fig. 1); pumps replaced after 7 days of artifical perilymph; animal info (pigmented, 400-500g., acoustic trauma, gunshot); tissue perfusion (scala tympani).

P7905 Shir,A., Ogris,M., Wagner,E., Levitzki,A. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. PLOS MEDICINE 2006; 3(-1-):125-135. >>> RNA, double stranded; polyinosine-cytosine (poly IC) dsRNA; Non-viral vector; SC; Mice; 2001D; 2 days; Per communication with author on 8/11/07, "We did not have any particular problems with the anesthesia exposure. For the pump replacement procedure Ketamine/Xylazine 2% (0.85ml/0.15ml), 40-50 ul IP per mouse (25 gram) was used. Replacing of the pump was not problematic either. The skin was closed with a surgical clips which are less traumatic to the mice and are easily applied and removed." 2001D pump replaced every day for two days total duration; synthetic dsRNA was attached to a non-viral vector (PEI-PEG-EGF beta PEI-Mel complexes) with specificity to the epidermal growth factor receptor (EGFR); controls received vector only (no poly IC); tissue perfusion (intratumoral); cancer (glioblastoma, breast, adenocarcinoma).

P7971 Sosenko,I.R.S., Kallapur,S.G., Nitsos,I., Moss,T.J.M., Newnham,J.P., Ikegami,M., Jobe,A.H. IL-1 alpha causes lung inflammation and maturation by direct effects on preterm fetal lamb lungs. PEDIATRIC RESEARCH 2006; 60(-3-):294-298. >>> Interleukin-1, alpha, recomb. ovine; Intratracheal; Sheep (fetus); 2001D; ; Controls received mp w/ saline; animal info (merino, ewes + fetuses); silicone tubing used to collect lung fluid; vinyl tubing for intratracheal infusion; tissue perfusion (trachea).

P7994 Vavrek,R., Girgis,J., Tetzlaff,W., Hebert,G.W., Fouad,K. BDNF promotes connections of corticospinal neurons onto spared descending interneurons in spinal cord injured rats. Brain 2006; 129(--):1534-1545. >>> Brain-derived neurotrophic factor; NT-3; Albumin, rat serum; CSF/CNS (left motor cortex, intrathecal, cisterna magna); Rat; 2002; 14 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; SCI (spinal cord injury); dental cement used to secure cannula; NT-3 delivered intrathecally; tissue perfusion (left motor cortex).

P7988 Wang,X.Y., Engisch,K.L., Teichert,R.W., Olivera,B.M., Pinter,M.J., Rich,M.M. Prolongation of evoked and spontaneous synaptic currents at the neuromuscular junction after activity blockade is caused by the upregulation of fetal acetylcholine receptors. Journal of Neuroscience 2006; 26(-35-):8983-8987. >>> Tetrodotoxin; Saline; CSF/CNS (sciatic nerve); Mice; 1002; ; Controls received mp w/ vehicle; animal info (2-4 months old); silastic nerve cuff; tissue perfusion (sciatic nerve).

P8036 Yasuhara,T., Matsukawa,N., Hara,K., Yu,G.L., Xu,L., Maki,M., Kim,S.U., Borlongan,C.V. Transplantation of human neural stem cells exerts neuroprotection in a rat model of Parkinson's disease. Journal of Neuroscience 2006; 26(-48-):12497-12511. >>> Stem cell factor, human recomb.; PBS; CSF/CNS (striatum); Rat; 2004; 4 weeks; Animal info (Sprague-Dawley, 200-250 grams); neurodegenerative (Parkinson's Disease); behavioral study; tissue perfusion (striatum).