Targeted Delivery of Agents Using ALZET® Osmotic Pumps

Q4743 Shinohara,K., Hata,T. Post-acquisition hippocampal NMDA receptor blockade sustains retention of spatial reference memory in Morris water maze. Behavioural Brain Research 2014; 259(--):261-267. >>> AP5, D-; CSF, artificial; CSF/CNS (hippocampus); Rat; 1007D; 7 days; 14 days; Animal info (male, albino Wistar); pumps replaced every 7 days; Plastics One bilateral cannula used; Multiple pumps per animal (2); behavioral testing (morris water maze, probe test); tissue perfusion (hippocampus); Cannula placement verified via histological analysis; pumps primed for 24 hours in 37C saline; bilateral infusion;.

Q4727 Koneru,B., Bathina,C.S., Cherry,B.H., Mifflin,S.W. Mineralocorticoid receptor in the NTS stimulates saline intake during fourth ventricular infusions of aldosterone. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2014; 306(--):R61-R66. >>> Aldosterone; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 28 days; 1;Animal info (Wistar-Kyoto, 275g); tissue perfusion (fourth ventricle); cardiovascular;.

Q4703 Benadiba,M., Costa,I.D., Santos,R.L.S.R., Serachi,F.O., Silva,D.D., Colquhoun,A., Colquhoun,A. Growth inhibitory effects of the Diruthenium-Ibuprofen compound, [Ru(2)Cl(Ibp)(4)], in human glioma cells in vitro and in the rat C6 orthotopic glioma in vivo 1993. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 2014; 19(--):1025-1035. >>> Diruthenium-ibuprofen; Ethanol; CSF, artificial; CSF/CNS (intratumoral); Rat; 2002; 14 days; Animal info (female, Wistar, 250-350g); ALZET brain infusion kit used; 15% ethanol used; comparison of injection vs mp; cancer (glioma); tissue perfusion (intratumoral, glioma); "Using the orthotopic C6 model the effects of either chronic 14-day treatment by intra-peritoneal injection of chronic 14-day intra-tumour infusion by an Alzet osmotic pump attached to a brain infusion cannula were tested. Tumour growth was reduced by both routes of administration with the osmotic pump appearing to be the less harmful route in terms of haematological responses" pg 1033; Diruthenium-Ibuprofen aka RuIbp;.

Q4673 Zamykal,M., Martens,T., Matschke,J., Guenther,H.S., Kathagen,A., Schulte,A., Peters,R., Westphal,M., Lamszus,K., Lamszus,K. Inhibition of intracerebral glioblastoma growth by targeting the insulin-like growth factor 1 receptor involves different context-dependent mechanisms. NEURO-ONCOLOGY 2015; 17(--):1076-1085. >>> IMC-A12; Saline; CSF/CNS (intratumoral); Mice; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (Foxn1nu, 6-8 weeks old); cancer (glioblastoma); tissue perfusion (glioblastoma); IMC-A12 aka cixutumumab;.

Q4656 Yang,H.J., Kwon,D.Y., Kim,M.J., Kang,S., Moon,N.R., Daily,J.W., Park,S., Park,S. Red peppers with moderate and severe pungency prevent the memory deficit and hepatic insulin resistance in diabetic rats with Alzheimer's disease. Nutrition&Metabolism 2015; 12(--):U15-U26. >>> Amyloid protein, beta (25-35); CSF/CNS (hippocampus); Rat; 14 days; Controls received mp w/ scrambled amyloid protein; animal info (male, Sprague Dawley, 192 +/-30g, ); neurodegenerative (Alzheimer's disease); behavioral testing (locomotive activity, passive avoidance, Morris water maze); tissue perfusion (CA1 subregion of hippocampus); diabetes;.

Q4593 Stark,R., Reichenbach,A., Lockie,S.H., Pracht,C., Wu,Q.L., Tups,A., Andrews,Z.B., Andrews,Z.B. Acyl Ghrelin Acts in the Brain to Control Liver Function and Peripheral Glucose Homeostasis in Male Mice. Endocrinology 2015; 156(--):858-868. >>> Ghrelin, acyl; CSF, artificial; CSF/CNS (third ventricle); Mice; 1007D; 7 days; Controls received mp w/ vehicle; animal info (male, C57BL6); ALZET brain infusion kit 3 used; tissue perfusion (third ventricle);.

Q4425 Garofalo,S., D'Alessandro,G., Chece,G., Brau,F., Maggi,L., Rosa,A., Porzia,A., Mainiero,F., Esposito,V., Lauro,C., Benigni,G., Bernardini,G., Santoni,A., Limatola,C., Limatola,C. Enriched environment reduces glioma growth through immune and non-immune mechanisms in mice. Nature Communications 2015; 6(--):U26-U38. >>> Interleukin-15; brain-derived neurotrophic factor; PBS; CSF/CNS (striatum); Mice; 1007D; 7 days; Controls received mp w/ vehicle; animal info (male, C57BL6, 3 weeks or 2 months old); ALZET brain infusion kit 3 used; cancer (glioma, U87MG human); tissue perfusion (right striatum); immunology; pumps primed in 37C saline overnight;.

Q4405 do Carmo,J.M., da Silva,A.A., Hall,J.E., do Carmo,J.M. Role of hindbrain melanocortin-4 receptor activity in controlling cardiovascular and metabolic functions in spontaneously hypertensive rats. Journal of Hypertension 2015; 33(--):1201-1206. >>> SHU-9119; CSF/CNS (fourth ventricle); Rat; 2002; 10 days; Animal info (male, Spontaneously hypertensive or Wistar-Kyoto, 275-325g, 15-17 weeks old); behavioral testing (food intake); tissue perfusion (fourth ventricle); cardiovascular; Cannula placement verified via Evan's Blue dye; bp measured using radiotelemetry (DSI);.

Q4386 Cuevas,S., Yang,Y., Konkalmatt,P., Asico,L.D., Feranil,J., Jones,J., Villar,V.A., Armando,I., Jose,P.A., Cuevas,S. Role of Nuclear Factor Erythroid 2-Related Factor 2 in the Oxidative Stress-Dependent Hypertension Associated With the Depletion of DJ-1. Hypertension 2015; 65(--):1251-U173. >>> RNA, small interfering Drd2-; RNA, small interfering DJ-1-; TransIT; Kidney (subcapsular space); Mice; 1007D; 7 days; Controls received mp w/ non-silencing RNA; animal info (male, C57BL6J, uninephrectomized); used PE tubing #0007701 with surgical glue; pump sutured to abdominal wall; tissue perfusion (kidney).

Q4347 Carrier,N., Saland,S.K., Duclot,F., He,H., Mercer,R., Kabbaj,M., Kabbaj,M. The Anxiolytic and Antidepressant-like Effects of Testosterone and Estrogen in Gonadectomized Male Rats. Biological Psychiatry 2015; 78(--):259-269. >>> Fadrozole; Saline, sterile; CSF/CNS (dentate gyrus); Rat; 2004; ; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-270g, adult, orchidectomized); bilateral cannula used; behavioral testing (sucrose preference test, open field test); tissue perfusion (dentate gyrus); Cannula placement verified via a posteriori by sectioning on cryostat; bilateral infusion; coordinates; pumps primed for 48 hours in 37C sterile saline; used Plastics One cannula;.

Q4320 Barbier,E., Tapocik,J.D., Juergens,N., Pitcairn,C., Borich,A., Schank,J.R., Sun,H., Schuebel,K., Zhou,Z.F., Yuan,Q.P., Vendruscolo,L.F., Goldman,D., Heilig,M., Barbier,E. DNA Methylation in the Medial Prefrontal Cortex Regulates Alcohol-Induced Behavior and Plasticity. Journal of Neuroscience 2015; 35(--):6153-6164. >>> RG108; Cyclodextrin, 2-hydroxypropyl-B-; CSF/CNS; CSF/CNS (medial prefrontal cortex); Rat; 2004; 2002; 1 week; Controls received mp w/ vehicle; animal info (male, Wistar, 200-225g); 5% cyclodextrin used; behavioral testing (two bottle free choice; alcohol self-administration); tissue perfusion (medial prefrontal cortex); dependence;.

Q4233 Zhou,Y.B., Sun,H.J., Chen,D., Liu,T.Y., Han,Y., Wang,J.J., Tang,C.S., Kang,Y.M., Zhu,G.Q., Zhu,G.Q. Intermedin in Paraventricular Nucleus Attenuates Sympathetic Activity and Blood Pressure via Nitric Oxide in Hypertensive Rats. Hypertension 2014; 63(--):330-+. >>> Intermedin; AM22-52; CSF, artificial; CSF/CNS (paraventricular nucleus); Rat; 1004; 15 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 160-180g); ALZET brain infusion kit used; tissue perfusion (paraventricular nucleus); antihypertensive; cardiovascular; Cannula placement verified via 2% Evans Blue dye injection; Intermedin aka IMD; AM22-52 is an adrenomedulllin receptor antagonist; bp measured using tail cuff;.

Q4225 Zhang,F., Sun,H.J., Xiong,X.Q., Chen,Q., Li,Y.H., Kang,Y.M., Wang,J.J., Gao,X.Y., Zhu,G.Q., Zhu,G.Q. Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats. ACTA PHYSIOLOGICA 2014; 212(--):17-27. >>> Apelin-13; CSF/CNS (paraventricular nucleus); Rat; 1002; 15 days; Controls received mp w/ saline; animal info (male, Wistar Kyoto, 13 weeks old); tissue perfusion (paraventricular nucleus); cardiovascular; "Osmotic minipumps were used for continuously delivering chemicals at controlled rate for several weeks without the need for external connections, frequent handling or repeated dosing" pg 19; bp measured using tail cuff; hypertension;.

Q4217 Yuyama,K., Sun,H., Usuki,S., Sakai,S., Hanamatsu,H., Mioka,T., Kimura,N., Okada,M., Tahara,H., Furukawa,J., Fujitani,N., Shinohara,Y., Igarashi,Y., Yuyama,K. A potential function for neuronal exosomes: Sequestering intracerebral amyloid-beta peptide. FEBS Letters 2015; 589(--):84-88. >>> Exosome; PBS; CSF/CNS (hippocampus); Mice; 14 days; Animal info (APP or C57BL6J, 13 months old); ALZET brain infusion kit used; neurodegenerative (Alzheimer's disease); tissue perfusion (hippocampus);.

Q4216 Yuyama,K., Sun,H., Sakai,S., Mitsutake,S., Okada,M., Tahara,H., Furukawa,J., Fujitani,N., Shinohara,Y., Igarashi,Y., Igarashi,Y. Decreased Amyloid-beta Pathologies by Intracerebral Loading of Glycosphingolipid-enriched Exosomes in Alzheimer Model Mice. Journal of Biological Chemistry 2014; 289(--):24488-24498. >>> Exosome solution, glycosphingolipid-enriched; PBS; CSF/CNS (hippocampus); Mice (trangenic); 1002; 14 days; Controls received mp w/ vehicle; animal info (APP, 4 months old); ALZET brain infusion kit 3 used; neurodegenerative (Alzheimers); tissue perfusion (hippocampus);.

Q4184 Wu,Y.W., Tu,Q.S., Valverde,P., Zhang,J., Murray,D., Dong,L.Q., Cheng,J., Jiang,H., Rios,M., Morgan,E., Tang,Z.H., Chen,J.K., Chen,J.K. Central adiponectin administration reveals new regulatory mechanisms of bone metabolism in mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2014; 306(--):E1418-E1430. >>> Adiponectin, globular; adiponectin, full-length; CSF, artificial; CSF/CNS (third ventricle); Mice; 28 days; Controls received mp w/ vehicle; animal info (male, APN-KO or WT, 20-25g, 4-6 weeks old); ALZET brain infusion kit 2 used; tissue perfusion (third ventricle); cyanoacrylate adhesive; Cannula placement verified via micrograph imaging; used tygon tubing to attach BIK cannula to pump;.

Q4181 Wu,K.L.H., Chao,Y.M., Tsay,S.J., Chen,C.H., Chan,S.H.H., Dovinova,I., Chan,J.Y.H., Chan,J.Y.H. Role of Nitric Oxide Synthase Uncoupling at Rostral Ventrolateral Medulla in Redox-Sensitive Hypertension Associated With Metabolic Syndrome. Hypertension 2014; 64(--):815-+. >>> Tempol; coenzyme Q10; CSF, artificial; CSF/CNS (cisterna magna); Rat; 1007D; 2 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley 8 weeks old, 235-296g); post op. care (1000 IU IM); tissue perfusion (cisterna magna); cardiovascular; diabetes; used PE-10 catheter;.

Q4173 Wang,Y.K., Shen,D., Hao,Q., Yu,Q., Wu,Z.T., Deng,Y., Chen,Y.F., Yuan,W.J., Hu,Q.K., Su,D.F., Wang,W.Z., Wang,W.Z. Overexpression of angiotensin-converting enzyme 2 attenuates tonically active glutamatergic input to the rostral ventrolateral medulla in hypertensive rats. American Journal of Physiology-Heart and Circulatory Physiology 2014; 307(--):H182-H190. >>> A779; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 1007D; 1 week; Controls received mp w/ vehicle; animal info (male, SHR and WKY); tissue perfusion (fourth ventricle);.

Q4141 Tona,Y., Hamaguchi,K., Ishikawa,M., Miyoshi,T., Yamamoto,N., Yamahara,K., Ito,J., Nakagawa,T., Nakagawa,T. Therapeutic potential of a gamma-secretase inhibitor for hearing restoration in a guinea pig model with noise-induced hearing loss. BMC NEUROSCIENCE 2014; 15(--):U1-U8. >>> Antibody, anti-aminoprocalcitonin; Saline; IP; Rat; 2001D; 14 days; Controls received mp w/ control antibody; animal info (male, Wistar, 280-300g); tissue perfusion (peritoneum); immunology; peptides; Catheter used to cannulate peritoneum;.

Q4128 Tan,M.S., Tan,L., Jiang,T., Zhu,X.C., Wang,H.F., Jia,C.D., Yu,J.T., Tan,L. Amyloid-beta induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer's disease. Cell Death&Disease 2014; 5(--):U228-U239. >>> Scavenger receptor class B, type I; CSF/CNS (hippocampus); Rat; 1004; 8 weeks; Animal info (male, Sprague Dawley, 250-350g); tissue perfusion (CA3 area of hippocampus); immunology; brain tissue distribution; Scavenger receptor class B, type I akd SR-BI;.

Q3963 Lee,J., Stanley,J.A., McCracken,J.A., Banu,S.K., Arosh,J.A., Arosh,J.A. Intrauterine Coadministration of ERK1/2 Inhibitor U0126 Inhibits Interferon TAU Action in the Endometrium and Restores Luteolytic PGF(2alpha) Pulses in Sheep. Biology of Reproduction 2014; 91(--):U177-U185. >>> U0126; serum protein, ovine; interferon tau, recombinant ovine; DMSO; Intrauterine (uterine horn); Sheep (ewe); 2ML1; 6 days; Controls received mp w/ vehicle; animal info (female, Suffolk Ovis aries); 3% DMSO used; tissue perfusion (uterine horn); cyanoacrylate adhesive; used cyanoacrylate glue to anchor pump; interferon tau aka IFNT;.

Q3956 Larson,T.A., Thatra,N.M., Lee,B.H., Brenowitz,E.A., Brenowitz,E.A. Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain. Journal of Neuroscience 2014; 34(--):13066-13076. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 5 days; Animal info (male, F-344, 3 months old); behavioral testing (rotarod; open field test; forced swim test); tissue perfusion (fourth ventricle); immunology;.

Q3853 Desai,B.N., Harris,R.B.S., Harris,R.B.S. Leptin in the hindbrain facilitates phosphorylation of STAT3 in the hypothalamus. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2015; 308(--):E351-E361. >>> Leptin; CSF/CNS (third ventricle; fourth ventricle); Rat; 2002; 6 days; Controls received mp w/ saline; animal info (male, Sprague Dawley, 275-300g); Multiple pumps per animal (2); tissue perfusion (third ventricle; fourth ventricle); bilateral infusion; one cannula to third ventricle second cannula to fourth ventricle; used plastics one cannula;.

Q3815 Bochner,D.N., Sapp,R.W., Adelson,J.D., Zhang,S.Y., Lee,H.M., Djurisic,M., Syken,J., Dan,Y., Shatz,C.J., Shatz,C.J. Blocking PirB up-regulates spines and functional synapses to unlock visual cortical plasticity and facilitate recovery from amblyopia. Science Translational Medicine 2014; 6(--):U13-U22. >>> Paired immunoglobulin-like receptor B, soluble; BSA; PBS; CSF/CNS (primary visual cortex); Mice; 1002; 11 days; Controls received mp w/ vehicle; animal info (PirB -/-, P21); functionality of mp verified by staining for sPirB; tissue perfusion (primary visual cortex);.

Q3795 Aghazadeh,Y., Martinez-Arguelles,D.B., Fan,J.J., Culty,M., Papadopoulos,V., Papadopoulos,V. Induction of Androgen Formation in the Male by a TAT-VDAC1 Fusion Peptide Blocking 14-3-3epsilon Protein Adaptor and Mitochondrial VDAC1 Interactions. MOLECULAR THERAPY 2014; 22(--):1779-1791. >>> TVG167; TVS167; Water; IP; intratesticular; Rat; 24 hours; Controls received mp w/ vehicle; animal info (male, Sprague Dawley); tissue perfusion (testis); peptide; TVS167 and TVG167; pump infused at 1.0 ul/hr;.

Q3794 Adzovic,L., Lynn,A.E., D'Angelo,H.M., Crockett,A.M., Kaercher,R.M., Royer,S.E., Hopp,S.C., Wenk,G.L., Wenk,G.L. Insulin improves memory and reduces chronic neuroinflammation in the hippocampus of young but not aged brains. Journal of Neuroinflammation 2015; 12(--):U1-U10. >>> TPFC; CSF/CNS; Rat; 2001D; 4 weeks; Animal info (male, 200-250 g, F344 Fischer); ALZET brain infusion kit 2 used; TPFC, also known as Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin, is a carboranyl-containing chlorin of high boron content; cancer (glioma); tissue perfusion; convection-enhanced delivery.

Q3696 Wosiski-Kuhn,M., Erion,J.R., Gomez-Sanchez,E.P., Gomez-Sanchez,C.E., Stranahan,A.M., Stranahan,A.M. Glucocorticoid receptor activation impairs hippocampal plasticity by suppressing BDNF expression in obese mice. Psychoneuroendocrinology 2014; 42(--):165-177. >>> Corticosterone; Cyclodextrin, 2-hydroxypropyl-B;; CSF/CNS (hippocampus); Mice; 2 weeks; Controls received mp w/ vehicle and aCSF; animal info (male, C57BL6J or db/db, 5 weeks old); functionality of mp verified by hippocampal corticosterone levels; Multiple pumps per animal (2); behavioral testing (y-maze apparatus); tissue perfusion (bilateral hippocampi); immunology; Cannula placement verified via histology; used Plastics One bilateral cannula; bilateral infusion;.

Q3684 Berg,J.V., Vrohlings,M., Haller,S., Haimovici,A., Kulig,P., Sledzinska,A., Weller,M., Becher,B. Intratumoral IL-12 combined with CTLA-4 blockade elicits T cell-mediated glioma rejection. Journal of Experimental Medicine 2013; 210(-13-):2803-2811. >>> Interleukin-12, murine; PBS; CSF/CNS (intratumoral); Mice; 1004; 2004; 28 days; Controls received mp w/ vehicle; animal info (C57BL6); cancer (glioma); tissue perfusion (tumor; glioma); immunology; pumps primed at 37C; pumps explanted after 28 days;.

Q3661 Su,Q., Qin,D.N., Wang,F.X., Ren,J., Li,H.B., Zhang,M., Yang,Q., Miao,Y.W., Yu,X.J., Qi,J., Zhu,Z.M., Zhu,G.Q., Kang,Y.M., Qin,D.N. Inhibition of reactive oxygen species in hypothalamic paraventricular nucleus attenuates the renin-angiotensin system and proinflammatory cytokines in hypertension. TOXICOLOGY AND APPLIED PHARMACOLOGY 2014; 276(--):115-120. >>> Tempol; angiotensin II; CSF, artificial; saline, sterile; CSF/CNS (paraventricular nuclei); Rat; 1004; 4 weeks; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, adult, 250-275g); functionality of mp verified by increase bp; tissue perfusion (paraventricular nucleus); immunology; "The success rate of bilateral microinjection and vein infusion is respectively 65% and 78%." pg 116; bp measured using tail-cuff;.

Q3627 Sehgal,A., Chen,Q.M., Gibbings,D., Sah,D.W.Y., Bumcrot,D. Tissue-specific gene silencing monitored in circulating RNA. RNA-A PUBLICATION OF THE RNA SOCIETY 2014; 20(-2-):143-149. >>> RNA, small interfering; PBS; CSF/CNS (striatum); Rat; 7 days; Animal info (male, Sprague Dawley); neurodegenerative (Parkinson's disease); tissue perfusion (striatum); gene therapy; used Plastics One 30g cannula; primed overnight in 37C saline;.

Q3556 Ma,J.H., Choi,B.R., Chung,C., Min,S.S., Jeon,W.K., Han,J.S., Han,J.S. Chronic brain inflammation causes a reduction in GluN2A and GluN2B subunits of NMDA receptors and an increase in the phosphorylation of mitogen-activated protein kinases in the hippocampus. MOLECULAR BRAIN 2014; 7(--):U1-U10. >>> RNA, small interfering TREM2; CSF, artificial; CSF/CNS (dorsal third ventricle); Mice; 1004; 28 days; Controls received mp w/ vehicle or control siRNA; animal info (SAMP8, 6 months old); functionality of mp verified by decrease TREM2 protein levels pg. 1246; ALZET brain infusion kit used; neurodegenerative (Alzheimers); no stress (see pg. 1244); behavioral testing (Morris water maze); tissue perfusion (third ventricle); immunology;.

Q3510 Iijima,M., Yoshimizu,T., Shimazaki,T., Tokugawa,K., Fukumoto,K., Kurosu,S., Kuwada,T., Sekiguchi,Y., Chaki,S., Chaki,S. Antidepressant and anxiolytic profiles of newly synthesized arginine vasopressin V(1B) receptor antagonists: TASP0233278 and TASP0390325. British Journal of Pharmacology 2014; 171(--):3511-3525. >>> Allylglycine, 1-; CSF/CNS (hypothalamus); Rat; ; Animal info (male, Sprague Dawley, 211-246g); behavioral testing (forced swimming test, elevated plus maze, locomotor activity); tissue perfusion (dorsomedial hypothalamus); I-AG is a GAD inhibitor;.

Q3423 Brothers,H.M., Bardou,I., Hopp,S.C., Kaercher,R.M., Corona,A.W., Fenn,A.M., Godbout,J.P., Wenk,G.L. Riluzole Partially Rescues Age-Associated, but not LPS-Induced, Loss of Glutamate Transporters and Spatial Memory. Journal of Neuroimmune Pharmacology 2013; 8(-5-):1098-1105. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2004; 3 weeks; Controls received mp w/ vehicle; animal info (Fisher F-344, 3 months old); behavioral testing (Morris water maze); tissue perfusion (fourth ventricle); immunology;.

Q3414 Bardou,I., Kaercher,R.M., Brothers,H.M., Hopp,S.C., Royer,S., Wenk,G.L. Age and duration of inflammatory environment differentially affect the neuroimmune response and catecholaminergic neurons in the midbrain and brainstem. NEUROBIOLOGY OF AGING 2014; 35(-5-):1065-1073. >>> Endotoxin, LPS; CSF, artificial; CSF/CNS (fourth ventricle); Rat; 2006; 21 days; 56 days; Controls received mp w/ vehicle; animal info (male, F-344 rats, 3 months or 9 months or 23 months old); post op. care (lidocane 1% on wound; 2ML isotonic saline SC injection; 2% tylenol in drinking water 3 days after surgery); tissue perfusion (fourth ventricle); long-term study; immunology; LPS aka lipopolysaccharide;.

Q3410 Avolio,E., Mahata,S.K., Mantuano,E., Mele,M., Alo,R., Facciolo,R.M., Talani,G., Canonaco,M., Avolio,E. ANTIHYPERTENSIVE AND NEUROPROTECTIVE EFFECTS OF CATESTATIN IN SPONTANEOUSLY HYPERTENSIVE RATS: INTERACTION WITH GABAERGIC TRANSMISSION IN AMYGDALA AND BRAINSTEM. Neuroscience 2014; 270(--):48-57. >>> Catestatin; muscimol; Saline; PBS; CSF/CNS (amygdala, central nucleus); Rat; 2004; 15 days; Controls received mp w/ vehicle; animal info (male, Spontaneously Hypertensive Rat, 3, 6, or 9 months old); ALZET brain infusion kit 2 used; tissue perfusion (central nucleus of amygdala); cardiovascular; antihypertensive; peptides; Cannula placement verified via histological analysis and methylene blue injection; Catestatin aka CST; muscimol aka MUS; used acrylic dental cement; bp measured using using radiotelemetry; CST is a catecholamine release inhibitor; muscimol is a GABAa receptor agonist;.

Q3405 Armando,I., Villar,V.M., Jones,J.E., Lee,H., Wang,X.Y., Asico,L.D., Yu,P.Y., Yang,J., Escano,CS Jr, Pascua-Crusan,A.M., Felder,R.A., Jose,P.A., Villar,V.M. Dopamine D3 receptor inhibits the ubiquitin-specific peptidase 48 to promote NHE3 degradation. FASEB Journal 2014; 28(--):1422-1434. >>> GR103691; RNA, small interfering; Transfection reagent (TransIT); SC; kidney (subcapsular space); Mice; 1007D; 4 days; 7 days; Controls received mp w/ vehicle or nonsilencing "mock siRNA"; animal info (male, C57BL6J, adult); tissue perfusion (subcapsular space; kidney); gene therapy; antihypertensive; GR103691 is a D3R antagonist; used PE tubing #0007701; pump sutured to abdominal wall; surgical glue applied at puncture site to hold catheter tubing in place and to prevent leakage;.

Q3369 Wu,Q., Zheng,R.M., Srisai,D., McKnight,G.S., Palmiter,R.D. NR2B subunit of the NMDA glutamate receptor regulates appetite in the parabrachial nucleus. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2013; 110(-36-):14765-14770. >>> Lithium chloride; RO25-6981; bretazenil; Saline; CSF/CNS (fourth ventricle; parabrachial nucleus); Mice; 1002; 14 days; Controls received mp w/ vehicle or sham surgery; animal info (male, Agrp DTR/+ or WT); bilateral cannula used; dose-response (pg.14766, fig.3; pg.14769, fig.6); behavioral testing (food intake); tissue perfusion (fourth ventricle; parabrachial nucleus); Cannula placement verified; RO25-6981 is a NR2B antagonist; bilateral infusion;.

Q3353 Ohira,K., Takeuchi,R., Shoji,H., Miyakawa,T. Fluoxetine-Induced Cortical Adult Neurogenesis. Neuropsychopharmacology 2013; 38(-6-):909-920. >>> Ara-C; PBS; CSF/CNS (frontal lobe); Mice; 1004; 4 weeks; Controls received mp w/ vehicle; animal info (male, 3 months old, C57BL/6J); ALZET brain infusion kit 3 used; ischemia (cerebral); Multiple pumps per animal (two 1004 pumps, two Brain infusion kits); tissue perfusion (frontal lobe); brain tissue distribution;.

Q3351 Mohammad,M.G., Tsai,V.W.W., Ruitenberg,M.J., Hassanpour,M., Li,H., Hart,P.H., Breit,S.N., Sawchenko,P.E., Brownl,D.A. Immune cell trafficking from the brain maintains CNS immune tolerance. Journal of Clinical Investigation 2014; 124(-3-):1228-1241. >>> Fingolimod; DMSO; CSF/CNS (forebrain; hippocampus); SC (cervical lymph nodes); Mice (transgenic); 8 days; 4 weeks; Controls received mp w/ vehicle; animal info (female, C57BL6, 6-12 weeks old; 2D2 transgenic); ALZET brain infusion kit 3 used; 50% DMSO used; neurodegenerative (multiple sclerosis); tissue perfusion (forebrain, hippocampus, cervical lymph nodes); immunology; Cannula placement verified via Nissl staining;.

Q3338 Gmeiner,W.H., Lema-Tome,C., Gibo,D., Jennings-Gee,J., Milligan,C., Debinski,W. Selective anti-tumor activity of the novel fluoropyrimidine polymer F10 towards G48a orthotopic GBM tumors. Journal of Neuro-oncology 2014; 116(-3-):447-454. >>> F10; PBS; CSF/CNS (thalamus); Mice (nude); 1007D; 7 days; Controls received mp w/ vehicle; animal info (nu/nu, 7 weeks old); ALZET brain infusion kit 3 used; cancer (human glioblastoma G48a); dose-response (pg.451-452, fig.3b and fig.4); no stress (see pg. 451); tissue perfusion (posterior thalamus); immunology; "F10 does not penetrate the BBB in healthy mice (data not shown), thus intra-cranial (i.c.) administration of F10 results in high local concentrations that may be therapeutically beneficial. Dose-finding studies in nude mice demonstrate that F10 administered i.c. using an Alzet osmotic mini-pump at doses up to 200 mg/kg administered over 7 days are well-tolerated and do not cause damage to normal brain (Fig. 2b)." pg 451; Pumps primed in 37C saline overnight; F10 is a novel antitumor agent;.

Q3337 Erion,J.R., Wosiski-Kuhn,M., Dey,A., Hao,S., Davis,C.L., Pollock,N.K., Stranahan,A.M. Obesity Elicits Interleukin 1-Mediated Deficits in Hippocampal Synaptic Plasticity. Journal of Neuroscience 2014; 34(-7-):2618-2631. >>> IL-1 receptor antagonist; CSF, artificial; CSF/CNS (hippocampus); Mice; 2 weeks; Animal info (male, db/db C57BL6J); functionality of mp verified by ELISA of hippocampal IL1ra; behavioral testing (spatial recognition memory testing, novel-object preference, treadmill running); tissue perfusion (hippocampus); immunology; diabetes; Cannula placement verified via histology; Used Plastics One cannula.

Q3336 Cordeira,J.W., Felsted,J.A., Teillon,S., Daftary,S., Panessiti,M., Wirth,J., Sena-Esteves,M., Rios,M. Hypothalamic Dysfunction of the Thrombospondin Receptor alpha2beta-1 Underlies the Overeating and Obesity Triggered by Brain- Derived Neurotrophic Factor Deficiency. Journal of Neuroscience 2014; 34(-2-):554-565. >>> Gabapentin; Saline; CSF/CNS (ventromedial hypothalamus); Mice; 1004; 10 days; Controls received mp w/ vehicle; animal info (male, BDNF 2L/2LCk-Cre, 8-10 weeks old); behavioral testing (feeding, locomotor activity); tissue perfusion (ventromedial hypothalamus); Cannula placement verified via post hoc histological analysis ;.

Q3335 Cho,K.J., Kim,H.W., Cheon,S.Y., Lee,J.E., Kim,G.W. Apoptosis signal-regulating kinase-1 aggravates ROS-mediated striatal degeneration in 3-nitropropionic acid-infused mice. Biochemical and Biophysical Research Communications 2013; 441(-2-):280-285. >>> RNA, small interfering, ASK1; Nitropropanoic acid, 3-; Saline; CSF/CNS (striatum); SC; Mice (transgenic); 1007D; 7 days; Controls received mp w/ vehicle; animal info (male, SOD1-tg); functionality of mp verified by Western blotting; Multiple pumps per animal (2); neurodegenerative (apoptotic striatal degeneration); behavioral testing (rotarod test); tissue perfusion (striatum); 3-NP delivered SC, ASK1-siRNA delivered CSF/CNS.

Q3328 Biddlestone-Thorpe,L., Sajjad,M., Rosenberg,E., Beckta,J.M., Valerie,N.C.K., Tokarz,M., Adams,B.R., Wagner,A.F., Khalil,A., Gilfor,D., Golding,S.E., Deb,S., Temesi,D.G., Lau,A., O'Connor,M.J., Choe,K.S., Parada,L.F., Lim,S.K., Mukhopadhyay,N.D., Valerie,K. ATM Kinase Inhibition Preferentially Sensitizes p53-Mutant Glioma to Ionizing Radiation. Clinical Cancer Research 2013; 19(-12-):3189-3200. >>> KU-60019; PBS; CSF/CNS (intratumoral); Mice (nude); 1007D; 1002; 2002; 7 days; 19 days; Controls received mp w/ vehicle; animal info (female, nude, athymic, 15-20g, 5-6 weeks old); ALZET brain infusion kit 3 used; cancer (glioma); tissue perfusion (glioma); "To reach meaningful drug concentrations of KU-60019 within the tumor, the BBB/BTB need to be bypassed or drugs administered locally. Both osmotic pumps, as well as clinically used CED, partially bypass the BBB/BTB and deliver drugs directly to the tumor to improve efficacy and reduce potential systemic toxicity" pg3194; KU-60019 is a kinase inhibitor.

Q3248 Morishima,Y., Toigawa,M., Ohmura,N., Yoneda,T., Tagane,Y., Hata,Y. Critical Period of Experience-Driven Axon Retraction in the Pharmacologically Inhibited Visual Cortex. Cerebral Cortex 2013; 23(-10-):2423-2428. >>> Muscimol; Ringer's solution; CSF/CNS (primary visual cortex); Cat (kitten); 2001; 2002; 7 days; 8 days; Animal info (3 age groups: early phase P22-26, late phase P40-60, and adults >P500); post op. care (xylocane pump spray; entrofloxacin 5 mg/kg); tissue perfusion (primary visual cortex);.

Q3245 Montarolo,F., Parolisi,R., Hoxha,E., Boda,E., Tempia,F. Early Enriched Environment Exposure Protects Spatial Memory and Accelerates Amyloid Plaque Formation in APP(Swe)/PS1(L166P) Mice. PLoS One 2013; 8(-7-):U653-U666. >>> Tetrodotoxin; Saline; CSF/CNS (dorsal hippocampus); Mice (transgenic); 1004; 4 weeks; Controls received mp w/ vehicle; animal info (APP swe/PSI L166P, 2 months old); neurodegenerative (Alzheimer's); behavioral testing (Morris water maze, eight radial arm maze, escape latency); tissue perfusion (hippocampus); used dental cement to attach cannula; polyvinyl tubing.

Q3243 Miki,T., Hirai,H., Takahashi,T. Activity-Dependent Neurotrophin Signaling Underlies Developmental Switch of Ca(2+) Channel Subtypes Mediating Neurotransmitter Release. Journal of Neuroscience 2013; 33(-48-):18755-18763. >>> Tetrodotoxin; K252a; Saline; CSF/CNS (cerebellum); Rat (neonate); 1007D; 7 days; Controls received mp w/ vehicle; animal info (Wistar/ST, Postnatal day 6); ALZET brain infusion kit 1 used; tissue perfusion (cerebellum); teratology; good methods(pg. 18757); Pump implantation shown figure 6A pg.P18761. K252a is a CaM kinase and phosphorylase inhibitor;.

Q3212 Eberstaal,S., Sanden,E., Fritzell,S., Darabi,A., Visse,E., Siesjoe,P. Intratumoral COX-2 inhibition enhances GM-CSF immunotherapy against established mouse GL261 brain tumors. International Journal of Cancer 2014; 134(-11-):2748-2753. >>> Parecoxib; valdecoxib; IP; CSF/CNS (intratumoral); Mice; 1004; 7 days; 34 days; Animal info (C57BL/6, female, 8-10 wks old); cancer; tissue perfusion (intratumoral).

Q3185 Donegan,J.J., Girotti,M., Weinberg,M.S., Morilak,D.A. A Novel Role for Brain Interleukin-6: Facilitation of Cognitive Flexibility in Rat Orbitofrontal Cortex. Journal of Neuroscience 2014; 34(-3-):953-962. >>> Antibody, anti-IL-6; Saline; CSF/CNS (orbitofrontal cortex); Rat; 1004; ; Controls received mp w/ goat IgG; animal info (male, Sprague Dawley, adult, 220-240g); behavioral testing (learning); tissue perfusion (orbitofrontal cortex); immunology; Cannula placement verified via histology; Used dual-injection, osmotic pump compatible cannulae from Plastics One.

Q3180 Asavapanumas,N., Ratelade,J., Papadopoulos,M.C., Bennett,J.L., Levin,M.H., Verkman,A.S. Experimental mouse model of optic neuritis with inflammatory demyelination produced by passive transfer of neuromyelitis optica-immunoglobulin G. Journal of Neuroinflammation 2014; 11(-;-):U1-U11. >>> Antibody, rAb-53; complement, human; CSF/CNS (optic chiasm); Mice; 1003D; 3 days; Controls received mp w/ non-NMO control IgG and complement; animal info (AQP4+/+ and AQP4-/-, 8-10 weeks old); tissue perfusion (optic chiasm); immunology; Cannula placement verified via Evan's blue dye; rAb-53 aka NMO-IgG;.

Q3117 Mantilla,C.B., Gransee,H.M., Zhan,W.Z., Sieck,G.C. Motoneuron BDNF/TrkB signaling enhances functional recovery after cervical spinal cord injury. Experimental Neurology 2013; 247(-;-):101-109. >>> Brain-derived neurotrophic factor;; CSF/CNS (intrathecal); Rat; 2002; 14 days; Animal info (male, Sprague Dawley, adult); spinal cord injury; post op. care (Acetominophen orally and buprenorphine IM for 3 days); pulsed delivery (delayed delivery for 3 days - aCSF only in catheter); tissue perfusion (C4 segment of spine); peptides; "Mini-osmotic pumps were successfully implanted for intrathecal delivery at the C4 level and functioned properly throughout the 14 day duration of the experiment." pg 103 "Differences in survival rates across groups were likely unrelated to the possible additional morbidity associated with intrathecal catheter and miniosmotic pump implantation, as the survival rate was 70% (28 out of 40) compared to 84% (26 out of 31) in rats not implanted with an intrathecal pump (p = 0.17)." pg 103; PE-10 intrathecal cannula, 10 cm.

Q3106 Lemley,C.O., Camacho,L.E., Vonnahme,K.A. Uterine Infusion of Melatonin or Melatonin Receptor Antagonist Alters Ovine Feto-Placental Hemodynamics During Midgestation. Biology of Reproduction 2013; 89(-2-):U24-U32. >>> Melatonin; Luzindole; DMSO; water; Intrauterine (uterine horn); Sheep (ewe); 2ML4; 28 days; Controls received mp w/ vehicle; functionality of mp verified by serum levels of melatonin taken; 45% DMSO used; stress/adverse reaction: (see pg.2); post op. care (For two days: flunixin meglumine 50 mg/ml IM twice a day; Penicillin G procain 300,000 u/ml once per day); tissue perfusion (uterus mesometrium); cardiovascular;.

Q3105 Landry,T.G., Fallon,J.B., Wise,A.K., Shepherd,R.K. Chronic neurotrophin delivery promotes ectopic neurite growth from the spiral ganglion of deafened cochleae without compromising the spatial selectivity of cochlear implants. Journal of Comparative Neurology 2013; 521(-12-):2818-2832. >>> Neurotrophin; Brain-derived neurotrophic factor; Ear (cochlea); Guinea pig; 2004; 28 days; Controls received mp w/ artificial perilymph; animal info (young adult, 300-600g); tissue perfusion (cochlea); peptides;.

Q3103 Kurata,S., Goto,T., Gunjigake,K.K., Kataoka,S., Kuroishi,K.N., Ono,K., Toyono,T., Kobayashi,S., Yamaguchi,K. Nerve Growth Factor Involves Mutual Interaction between Neurons and Satellite Glial Cells in the Rat Trigeminal Ganglion. ACTA HISTOCHEMICA ET CYTOCHEMICA 2013; 46(-2-):65-73. >>> Carbenoxolone; Saline; CSF/CNS (trigeminal ganglion); Rat; 2001; 3 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 200-250g); tissue perfusion (Trigeminal ganglion, lefy maxillary division); Carbenoxolone aka carbenoxolone, CBX;.

Q3093 Kermath,B.A., Riha,P.D., Sajjad,A., Gore,A.C. Effects of Chronic NMDA-NR2b Inhibition in the Median Eminence of the Reproductive Senescent Female Rat. Journal of Neuroendocrinology 2013; 25(-10-):887-897. >>> Ifenprodil; Tartaric acid; CSF/CNS (median eminence); Rat; 4 weeks; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 9-11 months, persistent oestrus); stress/adverse reaction: (see pg. 888. One female died after surgery); post op. care (Carpofen SC injection 5 mg/kg; Pramoxine HCl on incision); tissue perfusion (Median eminence); used ALZET PE-60 catheter..

Q3075 Cantinieaux,D., Quertainmont,R., Blacher,S., Rossi,L., Wanet,T., Noel,A., Brook,G., Schoenen,J., Franzen,R. Conditioned Medium from Bone Marrow-Derived Mesenchymal Stem Cells Improves Recovery after Spinal Cord Injury in Rats: An Original Strategy to Avoid Cell Transplantation. PLoS One 2013; 8(-8-):U18-U32. >>> Conditioned medium, bone marrow-derived mesenchimal stem cells; CSF/CNS (intrathecal); Rat; 1007D; 7 days; Controls received mp w/ control medium; animal info (female, Wistar, adult, 200g); spinal cord injury; post op. care (manually emptied bladder until rats regained control); behavioral testing (BBB open-field test, grid navagation test); tissue perfusion (spinal cord injury site); cardiovascular; immunology; Bone marrow-derived mesenchymal stem cells conditioned medium aka BMSC-CM; Pumps removed after 7 days.

Q3056 Brozoski,T.J., Wisner,K.W., Odintsov,B., Bauer,C.A. Local NMDA Receptor Blockade Attenuates Chronic Tinnitus and Associated Brain Activity in an Animal Model. PLoS One 2013; 8(-10-):U388-U398. >>> AP5, D-; Saline; CSF/CNS (paraflocculus); Rat; 2002; 2 weeks; Controls received mp w/ methylene dye; animal info (male, Long-Evans, 60 days old); functionality of mp verified by residual volume; tissue perfusion (paraflocculus); brain tissue distribution; Used PE-50 tubing. D-AP5 is an NMDA antagonist. Pumps anchored to muscle/connective tissue via nylon suture.

Q3055 Brazda,N., Voss,C., Estrada,V., Lodin,H., Weinrich,N., Seide,K., Mueller,J., Mueller,H.W. A mechanical microconnector system for restoration of tissue continuity and long-term drug application into the injured spinal cord. Biomaterials 2013; 34(-38-):10056-10064. >>> Antibody, human IgG; Saline; CSF/CNS (spinal cord, intrathecal); Rat; 2001; 3 days; 7 days; Controls received mp w/ vehicle; animal info (Female, Wistar, 200-230g); spinal cord injury; post op. care (Baytril for one week, manual bladder emptying twice per day); behavioral testing (locomotor behavior); tissue perfusion (spinal cord); pump attached to mechanical microconnector system (mMS).

Q2864 Fritzell,S., Sanden,E., Eberstaal,S., Visse,E., Darabi,A., Siesjoe,P. Intratumoral temozolomide synergizes with immunotherapy in a T cell-dependent fashion. CANCER IMMUNOLOGY IMMUNOTHERAPY 2013; 62(-9-):1463-1474. >>> Temozolomide; CSF/CNS (intratumoral); Mice; 1003D; 3 days; Animal info (C57BL/6, female, syngenic, 8-10 wks old); ALZET brain infusion kit 3 used; cyanoacrylate; tissue perfusion (intratumoral); temozolomide (TMZ) is an alkylating agent.

Q2832 Yang,Y., Zhang,Y.R., Cuevas,S., Villar,V., Escano,C., Asico,L.D., Yu,P.Y., Grandy,D.K., Felder,R.A., Armando,I., Jose,P.A. Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D(2) receptor-induced inhibition of NADPH oxidase. Free Radical Biology and Medicine 2012; 53(-3-):437-446. >>> RNA, small interfering, D2R; RNA, small interfering, PON2; Kidney (renal capsule); Mice; 1007D; 7 days; Control animals received mp w/ nonsilencing control RNA; animal info (D2R deficient, 6-8 mo old, uniphrectomy); PE tubing used (item #0007701); "The body of the minipump was placed in the area previously occupied by the kidney that was removed; stabilization was achieved by suturing (4-0 ethilon) the minipump to the lateral abdominal musculature close to it."; tissue perfusion (renal capsule).

Q2710 Talasila,K.M., Soentgerath,A., Euskirchen,P., Rosland,G.V., Wang,J., Huszthy,P.C., Prestegarden,L., Skaftnesmo,K.O., Sakariassen,P.O., Eskilsson,E., Stieber,D., Keunen,O., Brekka,N., Moen,I., Nigro,J.M., Vintermyr,O.K., Lund-Johansen,M., Niclou,S., Mork,S.J., Enger,P.O., Bjerkvig,R., Miletic,H. EGFR wild-type amplification and activation promote invasion and development of glioblastoma independent of angiogenesis. Acta Neuropathologica 2013; 125(-5-):683-698. >>> Cetuximab; CSF/CNS (intratumoral); Rat (nude); 2ML4; 4 weeks; Control animals received mp w/ PBS; animal info (rnu/rnu Rowett); ALZET brain infusion kit 2 used; convection enhanced delivery (CED); tissue perfusion (intratumoral).

Q2471 Villar,V.M., Jones,J.E., Armando,I., Asico,L.D., Escano,CS Jr, Lee,H., Wang,X.Y., Yang,Y., Pascua-Crusan,A.M., Palmes-Saloma,C.P., Felder,R.A., Jose,P.A. Sorting Nexin 1 Loss Results in D(5) Dopamine Receptor Dysfunction in Human Renal Proximal Tubule Cells and Hypertension in Mice. Journal of Biological Chemistry 2013; 288(-1-):152-163. >>> RNA, small interfering; Kidney; Mice; 7 days; Control animals received mp w/ vehicle or non-silencing mock siRNA; animal info (C57BL/6, BALB/c, nephrectomized, adult, male); Snx-1 specific siRNA; infusion rate of 0.5 ul/hr; tissue perfusion (kidney); PE catheter used.

Q2463 Chandramohan,V., Bao,X.H., Kaneko,M.K., Kato,Y., Keir,S.T., Szafranski,S.E., Kuan,C.T., Pastan,I.H., Bigner,D.D. Recombinant anti-podoplanin (NZ-1) immunotoxin for the treatment of malignant brain tumors. International Journal of Cancer 2013; 132(-10-):2339-2348. >>> Immunotoxin, NZ-1; immunotoxin, P588; PBS-HSA; CSF/CNS (intratumoral); Mice; 1003D; 3 days; Control animals received mp w/ vehicle; tissue perfusion (intratumoral); cancer (brain).

Q2425 Toyota,H., Shimogori,H., Sugahara,K., Yamashita,H. A novel treatment for vestibular disorder with FGLM-NH2 plus SSSR. Neuroscience Letters 2012; 526(-2-):128-132. >>> FGLM-NH2; SSSR; Ear (cochlea); Guinea pig; 2002; 14 days; Control animals received mp w/ artificial perilymph; animal info (male, Hartley); FGLM-NH2 also known as Phenylalanine-Glycine-Leucine-Methionine-Amide; SSSR also known as Serine-Serine-Serine-Arginine; tissue perfusion.

Q2347 Ricu,M.A., Ramirez,V.D., Paredes,A.H., Lara,H.E. Evidence for a Celiac Ganglion-Ovarian Kisspeptin Neural Network in the Rat: Intraovarian Anti-Kisspeptin Delays Vaginal Opening and Alters Estrous Cyclicity. Endocrinology 2012; 153(-10-):4966-4977. >>> P234; Intraovarian; Rat; 2004; 28 days; Control animals received mp w/ saline; animal info (Sprague Dawley, male, 30, 60 days old); P234 is a kisspeptin antagonist; "the pumps were left in the animal for a total of 50 d to control the estrous cycling activity." pg 4968; tissue perfusion (ovary).

Q2327 Chim,H., Miller,E., Gliniak,C., Alsberg,E. Stromal-cell-derived factor (SDF) 1-alpha in combination with BMP-2 and TGF-beta1 induces site-directed cell homing and osteogenic and chondrogenic differentiation for tissue engineering without the requirement for cell seeding. Cell and Tissue Research 2012; 350(-1-):89-94. >>> Stromal-cell-derived factor-1, alpha; bone morphogenetic protein 2; transforming growth factor-1, beta; IP (abdominal wall); Rat; 2004; 4 weeks; Negative control animals received no cytokines; animal info (Sprague Dawley, adult); "A custom-made apparatus for the constant delivery of cytokines was assembled consisting in a microneedle system and Alzet osmotic pump" pg 90; fig 1b, image of custom-made cytokine delivery apparatus; tissue perfusion (anterior abdominal wall).

Q2301 Nishijima,K., Shukunami,K., Yoshinari,H., Takahashi,J., Maeda,H., Takagi,h., Kotsuji,F. Interactions among pulmonary surfactant, vernix caseosa, and intestinal enterocytes: intra-amniotic administration of fluorescently liposomes to pregnant rabbits. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 2012; 303(-3-):L208-L214. >>> Liposomes, fluorescently labeled; coatasome EL-01-C, hydrated; DMSO; water, distilled; Intrauterine; Rabbit (fetus); 2ML1; 1 week; Control animals received mp w/ liposome alone; animal info (Japanese, White, 4.2-5.4 kg, teen); tissue perfusion (fetus); "5-cm sterile PE 60 silicone catheter with silicone flange was attached to each pump" pg L209; Fig 2, image of pump and catheter placement; multiple pumps used (2); teratology; liposomes diameter 30-90 nm;.

Q2262 Casazza,A., Kigel,B., Maione,F., Capparuccia,L., Kessler,O., Giraudo,E., Mazzone,M., Neufeld,G., Tamagnone,L. Tumour growth inhibition and anti-metastatic activity of a mutated furin-resistant Semaphorin 3E isoform. EMBO Molecular Medicine 2012; 4(-3-):234-250. >>> Semaphorin 3E; IP; Mice; 2002; 2 weeks; Controls received mp w/ mock saline; animal info (12-14 wks old, RT2); tissue perfusion (pancreas); catheter placement verified post mortem; "(silastic tubing) was passed into the peritoneal cavity and sutured to the abdominal wall to reach directly the pancreas." data supplement; protein.

Q2166 Ganchou,F.P., Mankey,J.E., Goravanahally,M.P., Inskeep,E.K., Flores,J.A. EFFECT OF CHRONIC ADMINISTRATION OF ENDOTHELIN RECEPTOR TYPE A ANTAGONIST (BQ-610) ON FUNCTIONAL LIFESPAN OF THE CORPUS LUTEUM IN SHEEP. REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS 2012; 22(-4-):321-331. >>> BQ-610; Methanol; saline; Intraovarian (corpus luteum); Sheep (ewe); 2002; 14 days; Controls received mp w/ vehicle; animal info (nonpregnant, Suffolk); tissue perfusion (corpus luteum); BQ-610 is an endothelin receptor type A antagonist.

Q2057 Dorniak,P., Bazer,F.W., Wu,G.Y., Spencer,T.E. Conceptus-Derived Prostaglandins Regulate Endometrial Function in Sheep. Biology of Reproduction 2012; 87(-1-):U80-U86. >>> Interferon, tau, recomb. ovine; meloxicam; PGE2, ovine serum; PGF2a, ovine serum; PGI2, ovine serum; Ethanol; saline; Intrauterine (uterine horn); Sheep (ewe); 2ML1; 5 days; Controls received mp w/ vehicle; animal info (Mature Rambouillet); tissue perfusion (intrauterine); multiple pumps used (2); enzyme inhibitor (prostaglandin synthase two); PGI2 is also called iloprost.

Q2056 Dorniak,P., Welsh,TH Jr, Bazer,F.W., Spencer,T.E. Endometrial HSD11B1 and Cortisol Regeneration in the Ovine Uterus: Effects of Pregnancy, Interferon Tau, and Prostaglandins. Biology of Reproduction 2012; 86(-4-):U106-U115. >>> Interferon, tau, recomb. ovine; meloxicam; PGE2, ovine serum; PGF2a, ovine serum; PGI2, ovine serum; Ethanol; saline; Intrauterine (uterine horn); Sheep (ewe); 2ML1; 7 days; Controls received mp w/ vehicle; animal info (Mature Rambouillet); good methods (pg 2); vinyl tubing used (0007760); 2% ethanol used; enzyme inhibitor (prostaglandin synthase two); tissue perfusion (intrauterine); PGI2 is also called iloprost.

Q1975 Kotipatruni,R.R., Nalla,A.K., Asuthkar,S., Gondi,C.S., Dinh,D.H., Rao,J.S. Apoptosis Induced by Knockdown of uPAR and MMP-9 is Mediated by Inactivation of EGFR/STAT3 Signaling in Medulloblastoma. PLoS One 2012; 7(-9-):U893-U905. >>> RNA, small hairpin; CSF/CNS (intratumoral); Mice (nude); 2001; ; Animal info (nude); tissue perfusion (tumor); cancer.

Q1915 Sly,D.J., Hampson,A.J., Minter,R.L., Heffer,L.F., Li,J., Millard,R.E., Winata,L., Niasari,A., O'Leary,S.J. Brain-Derived Neurotrophic Factor Modulates Auditory Function in the Hearing Cochlea. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY 2012; 13(-1-):1-16. >>> Brain-derived neurotrophic factor; Ear (cochlea); Guinea pig; 2004; 4 weeks; Controls received mp w/ Ringers solution; animal info (adult, male, Dunkin-Hartley pigmented, 233-815 g); post op. care (buprenorphine); "Polymers, particularly hydrogels that may be applied directly to the round window, were considered... However, most have a release profile that varies over time, so instead we chose to place a cannula attached to a mini-osmotic pump directly onto the round window." pg 2; tissue perfusion (cochlea, round window niche).

Q1903 Cuevas,S., Zhang,Y.R., Yang,Y., Escano,C., Asico,L., Jones,J.E., Armando,I., Jose,P.A. Role of Renal DJ-1 in the Pathogenesis of Hypertension Associated With Increased Reactive Oxygen Species Production. Hypertension 2012; 59(-2-):446-452. >>> RNA, small interfering, DJ-1; In vivo transfection agent; Kidney; Mice; 1007D; 7 days; Controls received mp w/ non-silencing RNA; animal info (F1 hybrid; wt, 6-8 mo old, uninephrectomized, adult, male, C57BL/6J); ALZET polyethylene tubing set used (0007701); tissue perfusion (kidney); "The osmotic pump was sutured to the abdominal wall to prevent excessive movement of the pump" pg 447.

Q1685 Fransson,A., Maruyama,J., Miller,J.M., Ulfendahl,M. Post-Treatment Effects of Local GDNF Administration to the Inner Ears of Deafened Guinea Pigs. Journal of Neurotrauma 2010; 27(-9-):1745-1751. >>> Glial-derived neurotrophic factor; Ear (cochlea); Guinea pig; 2002; 4 weeks; Controls received mp w/ artificial perilymph; animal info (deafened); silcone tube used; tissue perfusion (cochlea); pump replaced after 2 weeks; post op. care (lidocaine).

Q1665 Watanabe,F., Kirkegaard,M., Matsumoto,S., Gont,C., Mannstroem,P., Ulfendahl,M., Fridberger,A. Signaling through erbB receptors is a critical functional regulator in the mature cochlea. European Journal of Neuroscience 2010; 32(-5-):717-724. >>> PD153035; 4557W; DMSO; artificial perilymph; Ear (cochlea); Guinea pig; 2002; ; Controls received mp w/ artificial perilymph; animal info (female, pigmented, 250-500 g); functionality of mp verified by residual volume; tissue perfusion (intracochlear); 0.1% DMSO used; enzyme inhibitor (tyrosine kinase).

Q1616 Lang,H.N., Jyothi,V., Smythe,N.M., Dubno,J.R., Schulte,B., Schmiedt,R.A. Chronic Reduction of Endocochlear Potential Reduces Auditory Nerve Activity: Further Confirmation of an Animal Model of Metabolic Presbyacusis. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY 2010; 11(-3-):419-434. >>> Furosemide; Ear (round window niche); Gerbil; 2004; 4 weeks; Controls were untreated; animal info (3-6 mo old, young adult); good methods, pg 421; tissue perfusion (round window).

Q1605 Robich,M.P., Matyal,R., Chu,L.M., Feng,J., Xu,S.H., Laham,R.J., Hess,P.E., Bianchi,C., Sellke,F.W. Effects of neuropeptide Y on collateral development in a swine model of chronic myocardial ischemia. Journal of Molecular and Cellular Cardiology 2010; 49(-6-):1022-1030. >>> Neuropeptide Y (3-36); Heparin; BSA; PBS; Intramyocardial; Pig (miniswine); 4 weeks; Controls received mp w/ placebo; animal info (Intact, adult, male, Yorkshire, miniswine); 2ML sized pump used; tissue perfusion (myocardium).

Q1551 Sherwin,J.R.A., Hastings,J.M., Jackson,K.S., Mavrogianis,P.A., Sharkey,A.M., Fazleabas,A.T. The Endometrial Response to Chorionic Gonadotropin Is Blunted in a Baboon Model of Endometriosis. Endocrinology 2010; 151(-10-):4982-4993. >>> Gonadotrophin, human chorionic, recomb.; Saline; SC; intrauterine; Monkey (baboon); 2ML1; ; Animal info (30 mo old, spontaneous endometriosis); tissue perfusion (oviductal lumen).

Q1509 Li,X., Mao,X.B., Hei,R.Y., Zhang,Z.B., Wen,L.T., Zhang,P.Z., Qiu,J.H., Qiao,L. Protective Role of Hydrogen Sulfide against Noise-Induced Cochlear Damage: A Chronic Intracochlear Infusion Model. PLoS One 2011; 6(-10-):U487-U492. >>> Sodium hydrosulfide; propargylglycine, DL-; Ear (cochlea); Rat; 2002; ; Controls received mp w/ artificial perilymph; animal info (Sprague Dawley, 250-350 g); tissue perfusion (cochlear); ALZET mouse jugular catheter used (#0007700); stress/adverse effects, pg e26728 "Two rats died of post-surgical infection, and one rat died of hemorrhage.".

Q1468 Girald,W., Collin,A., Izquierdo,M. Toxicity and delivery methods for the linamarase/linamarin/glucose oxidase system, when used against human glioma tumors implanted in the brain of nude rats. Cancer Letters 2011; 313(-1-):99-107. >>> Linamarase; linamarin; glucose oxidase; PBS; saline; CSF/CNS (intratumoral); SC; Rat (nude); 2001; 2ML1; 5, 7 days; animal info (Wistar, 200 g, rnu/rnu); tissue perfusion (intratumoral); ALZET brain infusion kit used; stress/adverse effects "we have found abnormal speed variations in the rate of delivery of the lin/ GO cocktail when an osmotic pump connected to a brain infusion flow moderator device by a catheter was used, maybe causing some of the premature deaths." pg 106; "Three rats died by toxicity 40, 48 and 72 h from the beginning of the treatment, and the systemic treatment was ended in all of them after the first death" pg 104; cancer (glioma).

Q1394 Wen,J.M., Grenz,A., Zhang,Y.J., Dai,Y.B., Kellems,R.E., Blackburn,M.R., Eltzschig,H.K., Xia,Y. A(2B) adenosine receptor contributes to penile erection via PI3K/AKT signaling cascade-mediated eNOS activation. FASEB Journal 2011; 25(-8-):2823-2830. >>> ; Intrapenile; Mice; ; Animal info (A2BR-deficient, C57CL/6); tissue perfusion (corpus cavernosum); ALZET mouse jugular catheter used; 25-gauge needle inserted into the right corpus cavernosum was connected to a pressure transducer and an amplifier unit; intracavernosal pressure (ICP) measurement.

Q1391 Warnecke,A., Scheper,V., Buhr,I., Wenzel,G.I., Wissel,K., Paasche,G., Berkingali,N., Jorgensen,J.R., Lenarz,T., Stoever,T. Artemin improves survival of spiral ganglion neurons in vivo and in vitro. NeuroReport 2010; 21(-7-):517-521. >>> Artemin; brain-derived neurotrophic factor; Ear (scala tympani); Guinea pig; 2002; 28 days; Negative controls received mp w/ artificial perilynph; animal info (deafened, pigmented, 250-450 g); pumps replaced after 14 days; tissue perfusion (scala tympani); pump connected to silicone-polyimide tubing.

Q1362 Toledo,R.N., Borin,A., Cruz,O.L.M., Ho,P.L., Testa,J.R.G., Fukuda,Y. The Action of Topical Basic Fibroblast Growth Factor in Facial Nerve Regeneration. OTOLOGY&NEUROTOLOGY 2010; 31(-3-):498-505. >>> Fibroblast growth factor, basic; albumin, human; Ringer lactate solution; CSF/CNS (facial nerve anastomosis); Rat; 2002; 14 days; Controls received mp w/ sodium heparin, human albumin in Ringer lactate solution; animal info (male, Wistar, adult); functionality of mp verified by residual volume; good methods, pg 499; stress/adverse reaction: (see pg. 501) "neuroma at the anastomosis", "hemoatoma at the surgical incision"; tissue perfusion (epineural anastomosis); vinyl catheter used; "We chose to use minipumps because this method allows better control of the quantity of bFGF delivered at the target site and is compatible with standard facial nerve surgical techniques. Furthermore, the isolate reservoir of the minipumps prevents the contact of the study drug with substances that could inactivate it" pg 502.

Q1236 Miyasaka,E.A., Raghavan,S., Gilmont,R.R., Mittal,K., Somara,S., Bitar,K.N., Teitelbaum,D.H. In vivo growth of a bioengineered internal anal sphincter: comparison of growth factors for optimization of growth and survival. PEDIATRIC SURGERY INTERNATIONAL 2011; 27(-2-):137-143. >>> Fibroblast growth factor-2; vascular endothelial growth factor-2; platelet-derived growth factor; SC; Mice; 1004; 28 days; Controls received mp with no growth factors; animal info (C57BL/6); good methods, pg 138; tissue perfusion (internal anal sphincter); silicone catheter used; "the osmotic pumps we used completed delivery of the growth factors by 28 days, which would clearly limit the duration of exposure to the growth factor, lessening the risk of malignancy" pg 143.

Q1229 Matavelli,L.C., Huang,J.Q., Siragy,H.M. (Pro)renin receptor contributes to diabetic nephropathy by enhancing renal inflammation. Clinical and Experimental Pharmacology and Physiology 2010; 37(-3-):277-282. >>> Handle region peptide; valsartan; Saline; Kidney (renal cortical interstitium); Rat; 2002; 14 days; Controls received mp w/ vehicle; animal info (Sprague Dawley. STZ-induced diabetes, male 230-260 g); peptides; good methods, pg 278; tissue perfusion (interstitium); HRP, also known as handle region peptide, is the decapeptide NH3-RILLKKMPSVCOOH; good methods, pg 278; "The osmotic minipump was implanted subcutaneously in the subscapular region of all rats. Thereafter, a midline laparotomy was performed and the left kidney was isolated. A PE-10 catheter connected to each minipump was tunnelled subcutaneously through a bevel-tipped stainless-steel tube to emerge into the abdominal cavity and the distal end of the catheter was placed under the left renal capsule and glued onto the surface of the kidney using Vetbond." pg 278.

Q1195 Leake,P.A., Hradek,G.T., Hetherington,A.M., Stakhovskaya,O. Brain-Derived Neurotrophic Factor Promotes Cochlear Spiral Ganglion Cell Survival and Function in Deafened, Developing Cats. Journal of Comparative Neurology 2011; 519(-8-):1526-1545. >>> Brain-derived neurotrophic factor, human; Perilymph, artificial; Ear (cochlea); Cat; 1002; 2004; 10 weeks; Controls received mp w/ vehicle; long-term study; animal info (adult, 4 wks old, deafened); functionality of mp verified via residual volume; pumps replaced after two weeks then after 28 days; tissue perfusion (cochlea); "The drug-delivery cannula within the cochlear implant... was connected to vinyl tubing..., which was connected to the regulator of the osmotic pump, which was implanted behind the right pinna."; artificial perilymph recipe.

Q1178 Kondo,T., Matsuoka,A.J., Shimomura,A., Koehler,K.R., Chan,R.J., Miller,J.M., Srour,E.F., Hashino,E. Wnt Signaling Promotes Neuronal Differentiation from Mesenchymal Stem Cells Through Activation of Tlx3. Stem Cells 2011; 29(-5-):836-846. >>> Wnt1; brain-derived neurotrophic factor; Ear (cochlea); Gerbil; 2004; 28 days; Controls received mp w/ saline or BDNF only; animal info (Mongolian, 4 mo old); pumps replaced after 72 hours; tissue perfusion (intracochlea).

Q1161 Kesanakurti,D., Chetty,C., Bhoopathi,P., Lakka,S.S., Gorantla,B., Tsung,A.J., Rao,J.S. Suppression of MMP-2 Attenuates TNF-alpha Induced NF-kappa B Activation and Leads to JNK Mediated Cell Death in Glioma. PLoS One 2011; 6(-5-):U239-U252. >>> RNA, small interfering, MMP-2; CSF/CNS (intratumorally); Mice (nude); 2004; ; Animal info (athymic, female, nu/nu, 4-6 wks old); cancer (glioma); tissue perfusion (intratumorally); MMP2 siRNA.

Q1158 Kawabata,S., Yang,W.L., Barth,R.F., Wu,G., Huo,T.Y., Binns,P.J., Riley,K.J., Ongayi,O., Gottumukkala,V., Vincente,M.G.H. Convection enhanced delivery of carboranylporphyrins for neutron capture therapy of brain tumors. Journal of Neuro-oncology 2011; 103(-2-):175-185. >>> Porphyrin, H2TCP; porphyrin, H2TBP; porphyrin, H2DCP; CSF/CNS (intratumoral); Rat (pregnant); 2001D; 24 hours; Controls received no treatment; animal info (Fischer, CD, pregnant, 220-240 g); comparison of convection enhanced delivery (CED) vs mp; cancer (glioma); tissue perfusion (intratumoral); Compounds also known as 5,10,15,20-tetra-(4-nido-carboranylphenyl) tetrabenzoporphyrin, 5,10,15,20-tetra-(4-nido-carboranylphenyl)porphyrin and 5,15- di-[3,5-(nido-carboranylmethyl)phenyl]porphyrin; "The animals that received of H2TBP by Alzet pump had longer MSTs than those that received it by CED (43.8 vs. 33.8 days), demonstrating that Alzet pump delivery was more effective than CED" pg 181.

Q1015 Colen,C.B., Shen,Y.M., Ghoddoussi,F., Yu,P.Y., Francis,T.B., Koch,B.J., Monterey,M.D., Galloway,M.P., Sloan,A.E., Mathupala,S.P. Metabolic Targeting of Lactate Efflux by Malignant Glioma Inhibits Invasiveness and Induces Necrosis: An In Vivo Study. NEOPLASIA 2011; 13(-7-):620-632. >>> Cyano-4-hydroxycinnamate, alpha; PBS; CSF/CNS (caudate patumen); Rat (nude); 2004; 28 days; Controls received mp w/ saline; animal info (8-10 wks old, 250-300 g, male); cancer (glioma); tissue perfusion (intratumoral); alpha cyano-4-hydroxy cyano-4-hydroxy also known as ACCA is a small-molecule inhibitor of lactate transport; Plastics One non-metal cannula used; MRI.

Q1005 Brosens,J.J., Hodgetts,A., Feroze-Zaidi,F., Sherwin,J.R.A., Fusi,L., Salker,M.S., Higham,J., Rose,G.L., Kajihara,T., Young,S.L., Lessey,B.A., Henriet,P., Langford,P.R., Fazleabas,A.T. Proteomic analysis of endometrium from fertile and infertile patients suggests a role for apolipoprotein A-I in embryo implantation failure and endometriosis. MOLECULAR HUMAN REPRODUCTION 2010; 16(-4-):273-285. >>> Gonadotrophin, human chorionic, recomb.; Oviductal; Monkey (baboon); 5 days; Animal info (cycling, female, 7-12 years old, 12-18 kg); tissue perfusion (oviduct).

Q0954 Abaamrane,L., Raffin,F., Schmerber,S., Sendowski,I. Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss. European Archives of Oto-rhino-laryngology 2011; 268(-7-):987-993. >>> Leupeptin; z-VAD-FMK; Ear (cochlea); Guinea pig; 2001; 7 days; Controls received no treatment; animal info (albino, 400-600 g); enzyme inhibitor (caspase); artificial perilymph solution recipe; "A miniature glass pipette with a ring of glue placed next to the tip to provide a leak-proof seal protecting the cochlea from contamination was connected to the catheter." pg 988; tissue perfusion.

Q0856 Huang,J.Q., Matavelli,L.C., Siragy,H.M. Renal (pro)renin receptor contributes to development of diabetic kidney disease through transforming growth factor-beta1 - connective tissue growth factor signalling cascade. Clinical and Experimental Pharmacology and Physiology 2011; 38(-4-):215-221. >>> Handle region peptide; valsartan; Kindey (renal cortex); Rat; 14 days; Controls received sham mp w/ saline; animal info (male, Sprague-Dawley, 230-260 g); tissue perfusion (renal cortex); peptides.


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“All the rats survived for the length of the experiments. The intratracheal pump was well tolerated and the procedure produced no overt complications.” (p. 123) Svetlecic et al., Lung 2004;182:119-134.