References on Teratology Research Using ALZET® Osmotic Pumps

16187 Talton, O. O., Pennington, K. A., Pollock, K. E., Bates, K., Ma, L., Ellersieck, M. R., Schulz, L. C. Maternal Hyperleptinemia Improves Offspring Insulin Sensitivity in Mice. Endocrinology 2016; 157(-7-):2636-48. >>> Leptin; Saline; SC; Mice (pregnant); 2004; Controls received mp w/ vehicle; animal info (female, WT); stress/adverse reaction: (see pg. 2645); teratology; diabetes; Dose (350 ng/h);.

16085 Zhao, S., Xie, P., Chen, J., Liu, L., Fan, H. A proteomic study on liver impairment in rat pups induced by maternal microcystin-LR exposure. Environ Pollut 2016; 212(--):197-207. >>> Microcystin-LR; Saline; IP; Rat (pregnant); 2004; Controls received mp w/ vehicle; animal info (Sprague Dawley, 12 weeks old, GD8); teratology; teratology; microcystin-LR aka MCLR; Dose (10 ug/kg/day);“It should be noted that the actualdelivered dose of MCLR decreased as the pregnancy progressed because the weight of the mother at GD8 was used to calculate the MCLR dose, and the body weight increased from this point throughout pregnancy.” pg 198.

16084 Zhao, L., Zhuang, J., Xu, F. Bronchopulmonary C-fibers'IL1RI contributes to the prolonged apneic response to intra-atrial injection of capsaicin by prenatal nicotinic exposure in rat pups. Toxicol Appl Pharmacol 2016; 303(--):58-64. >>> Nicotine; methyllycaconitine; Saline; SC; Rat; 2004; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 250-350g); pumps replaced every 28 days; Multiple pumps per animal (2); long-term study; teratology; MLA is an a7nAChR antagonist; methyllycaconitine aka MLA; Dose (nicotine 6 mg/kg/day; MLA 3 mg/kg/day);.

15878 Slotkin, T. A., Skavicus, S., Card, J., Stadler, A., Levin, E. D., Seidler, F. J. Developmental Neurotoxicity of Tobacco Smoke Directed Toward Cholinergic and Serotonergic Systems: More Than Just Nicotine. Toxicol Sci 2015; 147(-1-):178-89. >>> Nicotine; tobacco smoke extract; DMSO; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, non-pregnant, adult, Sprague Dawley); functionality of mp verified by plasma nicotine levels; dose-response (pg 181); teratology; Dose (Nicotine free base 0.2 or 2 mg/kg/day; TSE 0.18 mg/kg/day);.

15877 Singh, S. P., Chand, H. S., Gundavarapu, S., Saeed, A. I., Langley, R. J., Tesfaigzi, Y., Mishra, N. C., Sopori, M. L. HIF-1alpha Plays a Critical Role in the Gestational Sidestream Smoke-Induced Bronchopulmonary Dysplasia in Mice. PLoS One 2015; 10(-9-):e0137757. >>> Mecamylamine; Saline, sterile; SC; Mice; 2006; animal info (3-4 month sold); teratology;.

15861 Zhao, L., Zhuang, J., Zang, N., Lin, Y., Lee, L. Y., Xu, F. Prenatal nicotinic exposure upregulates pulmonary C-fiber NK1R expression to prolong pulmonary C-fiber-mediated apneic response. Toxicol Appl Pharmacol 2016; 290(--):107-15. >>> Nicotine; mecamylamine; methyllycaconitine; SC; Rat (pregnant); 2004; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 250-300g); pumps replaced on GD7; Multiple pumps per animal (2); no stress (see pg. 110-111); teratology; Dose (Nicotine 6 mg/kg/day; mecamylamine 0.03 mg/kg/day; methyllycaconitine 3 mg/kg/day);.

15769 Achikam Haima,, Christopher Albin-Brooks, Morgan Sherer, Emily Mills, Benedetta Leuner  Hormones and Behavior 2016; 77(--):124-131. >>> Citalopram HBr; Saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, GD); functionality of mp verified by residual volume; post op. care (ibuprofen 15 mg/kg in drinking water for 7 days); post op. care (ibuprofen 15 mg/kg in drinking water for 7 days); teratology; Therapeutic indication (post partum stress); Dose (10 mg/kg/day);.

15764 Sveinbjorn Gizurarson, Elisabet Run Sigurdardottir, Hamutal Meiri, Berthold Huppertz, Marei Sammar, Adi Sharabi-Nov, Maurizio Mandalá, George Osol  Fetal Diagnosis and Therapy 2016; 39(--):56-63. >>> Placental protein 13; SC; Rat (pregnant); 2ML1; Controls received mp w/ mutant PP13 ?(DelT221) or saline; animal info (female, Sprague Dawley, 12 weeks old, GD8); teratology; cardiovascular; bp measured using tail cuff; Dose (0.625 ng/hour); Industry authored (Telemarpe Ltd.);.

15751 Heloísa Helena Vilela Costa,, Fabiana Cardoso Vilela,, Alexandre Giusti-Paiva  Behav. Brain Res 2013; 257(--):31-38. >>> WIN 55,212-2; NaCl; tween, DMSO; CSF/CNS; Rat; 1007D; Controls received mp w/ vehicle; animal info (female, Wistar, 9 weeks old); 10% DMSO used; dose-response (pg 34); behavioral testing (fear conditioning, maternal behavior); teratology;“The continuous infusion of WIN in the CNS using an osmotic minipump in lactating dams eliminates the possibility that WIN affects the behavior of the offspring when offered in the milk. Thus, any behavioral change in offspring could be attributed only to changes inmaternal behavior from the administration of WIN in the dams.” pg 37; Cannula placement verified via Evans Blue dye postmortem; Dose (10 or 100 nmol/h);.

15747 Kang Chen,, Sakura Nakauchi,, Hailing Su,, Saki Tanimoto,, Katumi Sumikawa  Neuropharmacology 2016; 101(--):57-67. >>> Nicotine; Saline; SC; Rat (lactating); 2ML2; animal info (female, Sprague Dawley, ); teratology; Dose (6 mg/kg/day);.

15744 Jayne C. Charnock,, Mark R. Dilworth,, John D. Aplin,, Colin P. Sibley,, Melissa Westwood,, and Ian P. Crocker  Am J Physiol Endocrinol Metab 2016; (-310-):E24-E31. >>> Insulin-like growth factor 2, Leu27; HCl; SC; SC; Controls received mp w/ vehicle; animal info (female, eNOS -/-, E12.5); teratology; Dose (1 mg/kg/day); noted using"100ul miniosmotic pump (200D)"pgE25;.

15737 Kelsey Brooks and Thomas E. Spencer  Biology of Reproduction 2015; 92(2)(-47-):1-10. >>> oligonucleotdies, antisense morpholino; PBS; Intrauterine; Sheep (ewe, pregnant); 2ML1; Controls received mp w/ control oligonucleotides; animal info (female, Columbia Rambouillet); teratology; cyanoacrylate adhesive; used vinyl catheter tubing (0007760) to cannulate uterine lumen; pump affixed to mesosalpinx using cyanoacrylate glue;.

15097 Tain, Y.L., Huang, L.T., Chan, J.Y.H., Lee, C.T., Tain, Y.L. Transcriptome Analysis in Rat Kidneys: Importance of Genes Involved in Programmed Hypertension. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2015; 16(--):4744-4758. >>> L-NAME; SC; Rat (pregnant); Controls received mp w/ saline, iso-osmotic; animal info (female, Sprague Dawley); teratology; cardiovascular; bp measured using tail cuff;.

15088 Standen, P., Sferruzzi-Perri, A.N., Taylor, R., Heinemann, G., Zhang, J.V., Highet, A.R., Pringle, K.G., Owens, J.A., Kumarasamy, V., Lumbers, E.R., Roberts, C.T., Roberts, C.T. Maternal insulin-like growth factor 1 and 2 differentially affect the renin-angiotensin system during pregnancy in the guinea pig. GROWTH HORMONE&IGF RESEARCH 2015; 25(--):141-147. >>> Insulin-like growth factor-1; insulin-like growth factor 2; Acetic acid; SC; Guinea Pig; 2002; Controls received mp w/ vehicle; animal info (GD20); teratology; cardiovascular;.

15064 Powell, G.L., Levine, R.B., Frazier, A.M., Fregosi, R.F., Fregosi, R.F. Influence of developmental nicotine exposure on spike-timing precision and reliability in hypoglossal motoneurons. JOURNAL OF NEUROPHYSIOLOGY 2015; 113(--):1862-1872. >>> Nicotine bitartrate; SC; Rat (pregnant); Controls received mp w/ saline; animal info (Sprague Dawley, GD5); functionality of mp verified by plasma cotinine levels; teratology;.

15106 Nakauchi, S., Malvaez, M., Su, H.L., Kleeman, E., Dang, R., Wood, M.A., Sumikawa, K., Sumikawa, K. Early postnatal nicotine exposure causes hippocampus-dependent memory impairments in adolescent mice: Association with altered nicotinic cholinergic modulation of LTP, but not impaired LTP. NEUROBIOLOGY OF LEARNING AND MEMORY 2015; 118(--):178-188. >>> Nicotine; SC; Mice (pregnant); Controls received mp w/ saline; animal info (C57BL6, pregnant); behavioral testing (object location, object recognition memory task, elevated-plus maze; teratology;.

15029 Maneenil, G., Kemp, M.W., Kannan, P.S., Kramer, B.W., Saito, M., Newnham, J.P., Jobe, A.H., Kallapur, S.G., Kallapur, S.G. Oral, Nasal and Pharyngeal Exposure to Lipopolysaccharide Causes a Fetal Inflammatory Response in Sheep. PLoS One 2015; 10(--):U1091-U1101. >>> Endotoxin, LPS; Oral cavity; Sheep (ewe); Controls received mp w/ saline; animal info (Merino); teratology; immunology;.

15020 Ehrlich, D.E., Neigh, G.N., Bourke, C.H., Nemeth, C.L., Hazra, R., Ryan, S.J., Rowson, S., Jairam, N., Sholar, C.A., Rainnie, D.G., Stowe, Z.N., Owens, M.J., Neigh, G.N. Prenatal stress, regardless of concurrent escitalopram treatment, alters behavior and amygdala gene expression of adolescent female rats. NEUROPHARMACOLOGY 2015; 97(--):251-258. >>> Escitalopram oxalate; Saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, nulliparious Sprague Dalwey, 200-225g); behavioral testing (open field, social interaction, novel object recognition, elevated plus maze; teratology;.

15007 Li, J.Y., Bo, L., Zhang, P.J., Gao, Q.Q., Li, L.J., Tang, J.Q., Wu, C.L., Li, D.W., Xiao, J.P., Chen, J., Tao, J.Y., Mao, C.P., Xu, Z.C., Xu, Z.C. Exposure to Nicotine During Pregnancy and Altered Learning and Memory in the Rat Offspring. NICOTINE&TOBACCO RESEARCH 2015; 17(--):661-666. >>> Nicotine; SC; Rat (pregnant); 2ML4; Controls received mp w/ saline; animal info (female, Sprague Dawley, GD4, 300-350g); behavioral testing (morris water maze, escape latency, cross-platform); teratology;.

14999 Kumar, S., Palaia, T., Hall, C.E., Ragolia, L., Ragolia, L. Role of Lipocalin-type prostaglandin D(2) synthase (L-PGDS) and its metabolite, prostaglandin D(2), in preterm birth. PROSTAGLANDINS&OTHER LIPID MEDIATORS 2015; 118(--):28-33. >>> BWA868C; PGD2, 11-deoxy-11-methylene; Saline; Mice; 1004; Controls received mp w/ vehicle; animal info (L-PGDS); teratology; toxicology; BWA868C; PGD2, 11-deoxy-11-methylene are DP1 and DP2 receptor antagonists;.

14990 Kass, L., Durando, M., Altamirano, G.A., Manfroni-Ghibaudo, G.E., Luque, E.H., Munoz-de-Toro, M., Munoz-de-Toro, M. Prenatal Bisphenol A exposure delays the development of the male rat mammary gland. REPRODUCTIVE TOXICOLOGY 2015; 54(--):37-46. >>> Bisphenol A; DMSO; SC; Rat; 1002; Controls received mp w/ vehicle; animal info (female, WistarGD9); teratology; toxicology;.

14964 Heyne, G.W., Melberg, C.G., Doroodchi, P., Parins, K.F., Kietzman, H.W., Everson, J.L., nsen-Wilson, L.J., Lipinski, R.J., Lipinski, R.J. Definition of Critical Periods for Hedgehog Pathway Antagonist-Induced Holoprosencephaly, Cleft Lip, and Cleft Palate. PLoS One 2015; 10(--):U757-U770. >>> Cyclopamine; SC; Mice (pregnant); 2001D; Animal info (female, C57BL6J, GD8.25-9.4); teratology;.

14902 Cuffe, J.S.M., Steane, S., Moritz, K.M., Paravicini, T.M., Paravicini, T.M. Differential mRNA Expression and Glucocorticoid-Mediated Regulation of TRPM6 and TRPM7 in the Heart and Kidney throughout Murine Pregnancy and Development. PLoS One 2015; 10(--):U1394-U1410. >>> Dexamethasone sodium phosphate; corticosterone; Saline; SC; Mice (pregnant); 1003D; Controls received mp w/ saline; animal info (female, E12.5, C57Bl6J, 8-10 weeks old); teratology; cardiovascular;.

14891 Conceicao, E.P., Peixoto-Silva, N., Pinheiro, C.R., Oliveira, E., Moura, E.G., Lisboa, P.C., Lisboa, P.C. Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring. FOOD AND CHEMICAL TOXICOLOGY 2015; 78(--):52-59. >>> Nicotine; Saline; SC; Rat (lactating); 2ML2; Controls received mp w/ vehicle; animal info (female, Wistar, PN2); teratology; toxicology; dependence;.

14879 Chen, C.M., Chou, H.C., Huang, L.T., Chen, C.M. Maternal nicotine exposure during gestation and lactation induces kidney injury and fibrosis in rat offspring. PEDIATRIC RESEARCH 2015; 77(--):56-63. >>> Nicotine tartrate; Saline, sterile; SC; Rat (pregnant); 2ML2; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, pregnant, GD7); teratology; used sutures;.

14878 Chen, C.M., Chou, H.C., Huang, L.T., Chen, C.M. Maternal Nicotine Exposure Induces Epithelial-Mesenchymal Transition in Rat Offspring Lungs. Neonatology 2015; 108(--):179-187. >>> Nicotine; SC; Rat; 2ML2; 2ML4; Controls received mp w/ saline; animal info (female, Sprague Dawley); teratology; dependence;.

14838 Barreda, S., Kidder, I.J., Mudery, J.A., Bailey, E.F., Bailey, E.F. Developmental nicotine exposure adversely effects respiratory patterning in the barbiturate anesthetized neonatal rat. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2015; 208(--):45-50. >>> Nicotine bitartrate; Saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, embryonic day 5); post op. care (antibiotics Pen-48, Buprenex 0.5 mg/kg BID); teratology;.

14830 Aye, I.L.M.H., Rosario, F.J., Powell, T.L., Janssona, T., Aye, I.L.M.H. Adiponectin supplementation in pregnant mice prevents the adverse effects of maternal obesity on placental function and fetal growth. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2015; 112(--):12858-12863. >>> Adiponectin; PBS; SC; Mice (pregnant); 1003D; Controls received mp w/ PBS; animal info (female, C57BL6J); teratology; diabetes;.

14827 Ayraam, J., Cohen, G., Drago, J., Frappell, P.B., Ayraam, J. Prenatal nicotine exposure increases hyperventilation in alpha4-knock-out mice during mild asphyxia. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2015; 208(--):29-36. >>> Nicotine bitartrate; Water; SC; Mice; 1002; Animal info (female, a4 nAChR KO or WT, embryonic day14); teratology;.

14799 Rayen, I., Gemmel, M., Pauley, G., Steinbusch, H.W.M., Pawluski, J.L., Pawluski, J.L. Developmental exposure to SSRIs, in addition to maternal stress, has long-term sex-dependent effects on hippocampal plasticity. PSYCHOPHARMACOLOGY 2015; 232(--):1231-1244. >>> Fluoxetine; Propylenediol; saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, adult, 250-300g); 50% propylenediol used; teratology;“These implants also reduced the effect of stress associated with repeated injections or oral gavage.” pg 1233;.

14787 Zhao, S.J., Li, G.Y., Chen, J., Li, G.Y. A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring. Journal of Proteomics 2015; 114(--):197-213. >>> Microcystin-LR; Saline; SC; Rat; 2004; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, GD8); teratology;.

14779 Zhuang, J.G., Zhao, L., Zang, N., Xu, F.D., Xu, F.D. Prenatal nicotinic exposure augments cardiorespiratory responses to activation of bronchopulmonary C-fibers. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 2015; 308(--):L922-L930. >>> Nicotine; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 250-350g); pumps replaced on 7th day of gestation; teratology;.

14535 Zhu, E.D., Louis, L., Brooks, D.J., Bouxsein, M.L., Demay, M.B., Demay, M.B. Effect of Bisphosphonates on the Rapidly Growing Male Murine Skeleton. Endocrinology 2014; 155(--):1188-1196. >>> Clodronate; SC; Mice; Controls received mp w/ vehicle; animal info (male, C57BL6, 18 days old); teratology;.

14452 Vestal-Laborde, A.A., Eschenroeder, A.C., Bigbee, J.W., Robinson, S.E., Sato-Bigbee, C., Sato-Bigbee, C. The Opioid System and Brain Development: Effects of Methadone on the Oligodendrocyte Lineage and the Early Stages of Myelination. Developmental Neuroscience 2014; 36(--):409-421. >>> Methadone; Saline; SC; Rat (pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Pregnant, Sprague Dawley, GD7); teratology; dependence;.

14430 Tan, M.L., Shao, P., Friedhuber, A.M., van Moorst, M., Elahy, M., Indumathy, S., Dunstan, D.E., Wei, Y.Z., Dass, C.R., Dass, C.R. The potential role of free chitosan in bone trauma and bone cancer management. Biomaterials 2014; 35(--):7828-7838. >>> Chitosan; starch; Water; SC; Mice (pregnant); 1002; Controls received mp w/ vehicle; animal info (female, pregnant, Balb/c); cancer (osteosarcoma; breast cancer; colorectal cancer); no stress (see pg. 7831); teratology; cardiovascular; chitosan is a biopolymer derived from chitin; Dose (5 mg/kg/day);.

14424 Tain, Y.L., Huang, L.T., Lee, C.T., Chan, J.Y.H., Hsu, C.N., Tain, Y.L. Maternal Citrulline Supplementation Prevents Prenatal N(G)-Nitro-L-Arginine-Methyl Ester (L-NAME)-Induced Programmed Hypertension in Rats. Biology of Reproduction 2015; 92(--):U27-U33. >>> L-NAME; SC; Rat (pregnant); Animal info (female, Sprague Dawley, 10 weeks old); teratology; cardiovascular;.

14423 Tain, Y.L., Lee, C.T., Huang, L.T., Tain, Y.L. Long-Term Effects of Maternal Citrulline Supplementation on Renal Transcriptome Prevention of Nitric Oxide Depletion-Related Programmed Hypertension: The Impact of Gene-Nutrient Interactions. International Journal of Molecular Sciences 2014; 15(--):23255-23268. >>> L-NAME; SC; Rat (pregnant); Controls received mp w/ saline; animal info (female, Sprague Dawley, 10 weeks old); teratology; cardiovascular;.

14406 Slotkin, T.A., Skavicus, S., Levin, E.D., Seidler, F.J., Slotkin, T.A. Prenatal nicotine changes the response to postnatal chlorpyrifos: Interactions targeting serotonergic synaptic function and cognition. Brain Research Bulletin 2015; 111(--):84-96. >>> Nicotine; Water, bacteriostatic; SC; Rat (pregnant); 2ML2; Controls received mp w/ vehicle; animal info (female, pregnant, Sprague Dawley); teratology; used wound clips.

14405 Slotkin, T.A., Seidler, F.J., Slotkin, T.A. Prenatal nicotine alters the developmental neurotoxicity of postnatal chlorpyrifos directed toward cholinergic systems: Better, worse, or just"different?". Brain Research Bulletin 2015; 110(--):54-67. >>> Nicotine; Water, bacteriostatic; SC; Rat; 2ML2; Controls received mp w/ vehicle; animal info (female, pregnant, Sprague Dawley); teratology;"dosage determined by the initial body weights of the dams (215±2 g);control pumps contained bacteriostatic water and equivalent con-centrations of sodium bitartrate. Because weights increased with gestation, the dose rate fell accordingly to 2 mg/kg/day, but the dose rates remained well within the range that produces nicotine plasma levels"pg 55; used wound clips.

14379 Sakai, K., Kimura, O., Furukawa, T., Fumino, S., Higuchi, K., Wakao, J., Kimura, K., Aoi, S., Masumoto, K., Tajiri, T., Sakai, K. Prenatal administration of neuropeptide bombesin promotes lung development in a rat model of nitrofen-induced congenital diaphragmatic hernia. Journal of Pediatric Surgery 2014; 49(--):1749-1752. >>> Bombesin; IP; Rat (pregnant); 2002; Controls received mp w/ saline; animal info (female, Sprague Dawley, pregnant E14); teratology;.

14324 Nishimura, T., Higuchi, K., Sai, Y., Sugita, Y., Yoshida, Y., Tomi, M., Wada, M., Wakayama, T., Tamura, A., Tsukita, S., Soga, T., Nakashima, E., Nakashima, E. Fetal Growth Retardation and Lack of Hypotaurine in Ezrin Knockout Mice. PLoS One 2014; 9(--):U723-U731. >>> Hypotaurine; PBS; SC; Mice (pregnant); Controls received mp w/ vehicle; animal info (female, Ez+/-, GD8.5); functionality of mp verified by plasma levels; teratology;.

14317 Navarro, D., Alvarado, M., Morte, B., Berbel, D., Sesma, J., Pacheco, P., De Escobar, G.M., Bernal, J., Berbel, P., Berbel, P. Late Maternal Hypothyroidism Alters the Expression of Camk4 in Neocortical Subplate Neurons: A Comparison with Nurr1 Labeling. Cerebral Cortex 2014; 24(--):2694-2706. >>> Parathormone (1-84), rat; calcitonin, rat; Acetate buffer; SC; Rat; 2001; Animal info (female, Wistar, E0); replacement therapy (thyroidectomy); teratology;.

14285 Lo, J.O., Schabel, M.C., Roberts, V.H.J., Morgan, T.K., Rasanen, J.P., Kroenke, C.D., Shoemaker, S.R., Spindel, E.R., Frias, A.E., Lo, J.O. Vitamin C supplementation ameliorates the adverse effects of nicotine on placental hemodynamics and histology in nonhuman primates. American Journal of Obstetrics and Gynecology 2015; 212(--):U271-U278. >>> Nicotine bitartrate; Water, bacteriostatic; SC; Monkey (macaque, pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Rhesus, pregnant, GD26); functionality of mp verified by blood levels; pumps replaced every 3 weeks; post op. care (cefazolin 150 mg BID); long-term study; teratology; dependence;.

14279 Lim, R., Acharya, R., Delpachitra, P., Hobson, S., Sobey, C.G., Drummond, G.R., Wallace, E.M., Wallace, E.M. Activin and NADPH-oxidase in preeclampsia: insights from in vitro and murine studies. American Journal of Obstetrics and Gynecology 2015; 212(--):U456-U467. >>> Activin A, recombinant human; SC; Mice; 2001; Controls received mp w/ saline; animal info (female, C57BL6, 8-10 weeks old, GD10); functionality of mp verified by plasma levels; teratology; preeclampsia model;.

14223 Horn, E.R., Gabriel, M., Horn, E.R. Gender- Related Sensitivity of Development and Growth to Real Microgravity in Xenopus laevis. Journal of Experimental Zoology Part A-Ecological Genetics and Physiology 2014; 321(--):1-12. >>> Nettle powder; Water, spring; In vitro; Toad (tadpole); 2ML2; Animal info (Xenopus laevis); teratology; spaceflight;.

14726 Eifert, A.W., Wilson, M.E., Vonnahme, K.A., Camacho, L.E., Borowicz, P.P., Redmer, D.A., Romero, S., Dorsam, S., Haring, J., Lemley, C.O., Lemley, C.O. Effect of melatonin or maternal nutrient restriction on vascularity and cell proliferation in the ovine placenta. ANIMAL REPRODUCTION SCIENCE 2015; 153(--):13-21. >>> Melatonin; luzindole; DMSO; water; Intrauterine; Sheep (ewe; pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Western white face, GD62); functionality of mp verified by serum levels; 45% DMSO used; good methods (pg 15); no stress (see pg. 15); post op. care (BID IM injection flunixin meglumine; QD IP injection Penicillin G Procaine); teratology; cardiovascular; used 20 cm of PE 60 tubing; pumps primed overnight 37C saline with catheters;.

14712 az-Garcia, C., Johannesson, L., Shao, R.J., Bilig, H., Braennstroem, M., az-Garcia, C. Pregnancy after allogeneic uterus transplantation in the rat: perinatal outcome and growth trajectory. Fertility and Sterility 2014; 102(--):1545-U353. >>> Tacrolimus; NaCl; SC; Rat; 2ML4; 2004; Controls received mp w/ vehicle; animal info (female, Lewis, virgin); functionality of mp verified by blood levels; pumps replaced every 2 weeks; teratology;.

14694 Chou, H.C., Chen, C.M., Chen, C.M. Maternal nicotine exposure during gestation and lactation induces cardiac remodeling in rat offspring. REPRODUCTIVE TOXICOLOGY 2014; 50(--):4-10. >>> Nicotine; Saline; SC; Rat (pregnant); 2ML2; 2ML4; Controls received mp w/ vehicle; animal info (female, GD7); functionality of mp verified by blood levels; dose-response (pg 6); teratology; dependence; cardiovascular;.

14621 Dhimolea, E., Wadia, P.R., Murray, T.J., Settles, M.L., Treitman, J.D., Sonnenschein, C., Shioda, T., Soto, A.M., Soto, A.M. Prenatal Exposure to BPA Alters the Epigenome of the Rat Mammary Gland and Increases the Propensity to Neoplastic Development. PLoS One 2014; 9(--):U200-U208. >>> Bisphenol A; DMSO; SC; Rat (pregnant); Control animals received mp w/ vehicle; animal info (female, sexually mature, Wistar-Furth, 8wks old); 50% DMSO used; teratology.

14604 Negishi, T., Nakagami, A., Kawasaki, K., Nishida, Y., Ihara, T., Kuroda, Y., Tashiro, T., Koyama, T., Yoshikawa, Y., Negishi, T. Altered social interactions in male juvenile cynomolgus monkeys prenatally exposed to bisphenol A. NEUROTOXICOLOGY AND TERATOLOGY 2014; 44(--):46-52. >>> Bisphenol A; DMA; PEG; SC; Monkey (pregnant); 2004; Control animals received mp w/ vehicle; animal info (cynomolgus, 2.5-4.0 kg, 5-13 years old, GD20);"We administered BPA in pregnant females on gestational day 20 at 10µg/kg/day through pumps implanted to produce an exposure regimen with a steady level of circulating BPA without the stress of daily restraint essential for oral administration."pg 50; teratology; 50% DMA used; 50% PEG used.

13987 Wang, W., Irani, R.A., Zhang, Y.J., Ramin, S.M., Blackwell, S.C., Tao, L.J., Kellems, R.E., Xia, Y. Autoantibody-Mediated Complement C3a Receptor Activation Contributes to the Pathogenesis of Preeclampsia. Hypertension 2012; 60(-3-):712-U273. >>> SB290157; DMSO; SC; Mice (pregnant); 1007D; Controls received mp w/ vehicle; animal info (female, C57Bl6J, ); 50% DMSO used; teratology; cardiovascular; immunology; SB290757 is a non-peptide C3aR antagonist.

13977 Valdes, G., Schneider, D., Corthorn, J., Ortiz, R., Acuna, S., Padilla, O., Valdes, G. Administration of angiotensin II and a bradykinin B2 receptor blocker in midpregnancy impairs gestational outcome in guinea pigs. Reproductive Biology and Endocrinology 2014; 12(--):U1-U8. >>> Angiotensin II; bradyzide; SC; Guinea pig (pregnant); 2ML2; Controls received mp w/ saline; animal info (virgin, Pirbright white, 600g); teratology; cardiovascular; peptides; bp measured using neonatal blood pressure cuff; AngII determined to be subpressor dose via pilot study; pumps removed after 14 day infusion.

13963 Takeda, T., Fujii, M., Hattori, Y., Yamamoto, M., Shimazoe, T., Ishii, Y., Himeno, M., Yamada, H. Maternal Exposure to Dioxin Imprints Sexual Immaturity of the Pups through Fixing the Status of the Reduced Expression of Hypothalamic Gonadotropin-Releasing Hormone. MOLECULAR PHARMACOLOGY 2014; 85(-1-):74-82. >>> Gonadotropin-releasing hormone; Saline; HCl; CSF/CNS; Rat; 2002; Controls received mp w/ vehicle; animal info (male, Wistar, PND63); ALZET brain infusion kit 2 used; behavioral testing (sexual behavior); stability verified by (incubation of GnRH at 37C for 2 weeks - half of GnRH remains in unchanged form pg.78); teratology; Cannula placement verified via 0.1% infusion of bromophenol blue; 0.1 M HCl.

13959 Somm, E., Larvaron, P., de Looij, Y.V., Toulotte, A., Chatagner, A., Faure, M., Metairon, S., Mansourian, R., Raymond, F., Gruetter, R., Wang, B., Sizonenko, S.V., Hueppi, P.S. Protective effects of maternal nutritional supplementation with lactoferrin on growth and brain metabolism. PEDIATRIC RESEARCH 2014; 75(-1-):51-61. >>> Dexamethasone; SC; Rat (pregnant); Controls received mp w/ vehicle; animal info (female, Sprague Dawley OFA, gestational day 14); teratology; pumps used to induce intrauterine growth restriction; dose: 100 ug/kg/day.

13947 Zhang, D., Dragomir, A., Akay, Y.M., Akay, M., Akay, M. Nicotine exposure increases the complexity of dopamine neurons in the parainterfascicular nucleus (PIF) sub-region of VTA. Journal of NeuroEngineering and Rehabilitation 2014; 11(--):U1-U6. >>> Nicotine; Saline; SC; Rat; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, GD5, 280-300g); teratology; dependence;.

13915 Roguski, E.E., Chen, H., Sharp, B.M., Matta, S.G. Fostering itself increases nicotine self-administration in young adult male rats. Psychopharmacology 2013; 229(-2-):227-234. >>> Nicotine hydrogen tartrate; Water; Rat (pregnant); 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley); pumps replaced after P2 with higher dosage; behavioral testing (locomotor activity, nicotine self-administration); teratology; dependence;.

13887 O'Connell, B.A., Moritz, K.M., Walker, D.W., Dickinson, H. Treatment of pregnant spiny mice at mid gestation with a synthetic glucocorticoid has sex-dependent effects on placental glycogen stores. PLACENTA 2013; 34(-10-):932-940. >>> Dexamethasone; Saline; SC; Mice (pregnant); 1003D; Controls received mp w/ vehicle; animal info (female, spiny mouse, day 20 gestation); teratology; pumps removed after 72 hours;.

13885 Numakawa, T., Matsumoto, T., Ooshima, Y., Chiba, S., Furuta, M., Izumi, A., Ninomiya-Baba, M., Odaka, H., Hashido, K., Adachi, N., Kunugi, H. Impairments in Brain-Derived Neurotrophic Factor-Induced Glutamate Release in Cultured Cortical Neurons Derived from Rats with Intrauterine Growth Retardation: Possible Involvement of Suppression of TrkB/Phospholipase C-gamma Activation. Neurochemical Research 2014; 39(-4-):785-792. >>> Thromboxane2, analog; PBS; IP; Rat (pregnant); 2ML1; Controls received mp w/ vehicle; animal info (female, Long Evans, pregnant); teratology;.

13883 Nitsos, I., Newnham, J.P., Rees, S.M., Harding, R., Moss, T.J.M., Moss, T.J.M. The Impact of Chronic Intrauterine Inflammation on the Physiologic and Neurodevelopmental Consequences of Intermittent Umbilical Cord Occlusion in Fetal Sheep. REPRODUCTIVE SCIENCES 2014; 21(--):658-670. >>> Endotoxin, LPS; Amniotic sac; Sheep (fetus); 2ML4; Controls received mp w/ saline; animal info (female, pregnant, GD80); post op. care (benacillin); teratology; immunology; E.coli LPS 055:B5, 18 mg/ml;.

13849 Lipinski, R.J., Holloway, H.T., O'Leary-Moore, S.K., Ament, J.J., Pecevich, S.J., Cofer, G.P., Budin, F., Everson, J.L., Johnson, G.A., Sulik, K.K., Lipinski, R.J. Characterization of Subtle Brain Abnormalities in a Mouse Model of Hedgehog Pathway Antagonist-Induced Cleft Lip and Palate. PLoS One 2014; 9(--):U557-U563. >>> Cyclopamine; Cyclodextrin, 2-hydropropyl-B; sodium phosphate; citrate buffer; SC; Mice (pregnant); 2001D; Controls received mp w/ vehicle; animal info (female, C57BL6J, GD 8.25); 30% 2-hydropropyl-B-cyclodextrin used; no stress (see pg. e102604); teratology; cleft palate;.

13787 Gorini, C., Jameson, H., Woerman, A.L., Perry, D.C., Mendelowitz, D. Prenatal nicotine exposure enhances the trigeminocardiac reflex via serotonin receptor facilitation in brainstem pathways. Journal of Applied Physiology 2013; 115(-4-):415-421. >>> Nicotine; Rat (pregnant); Animal info (female, Sprague Dawley, adult, third day of gestation); teratology; dependence;.

13786 Gooi, J.H., Richardson, M.L., Jelinic, M., Girling, J.E., Wlodek, M.E., Tare, M., Parry, L.J. Enhanced Uterine Artery Stiffness in Aged Pregnant Relaxin Mutant Mice Is Reversed with Exogenous Relaxin Treatment. Biology of Reproduction 2013; 89(-1-):U76-U86. >>> Relaxin, human H2 recombinant; SC; Mice (pregnant); 1007D; Controls received mp w/ placebo; animal info (female, Rln -/-, 8 months old or 5 months old, day 12.5 of pregnancy); functionality of mp verified by blood plasma; teratology; cardiovascular;.

13658 Rayen, I., Steinbusch, H.W.M., Charlier, T.D., Pawluski, J.L. Developmental fluoxetine exposure facilitates sexual behavior in female offspring. Psychopharmacology 2014; 231(-1-):123-133. >>> Fluoxetine; Propylenediol; saline; SC; Rat; 2ML4; Controls received mp w/ vehicle; animal info (female, Sprague Dawley, 250-300g, postpartum); 50% propylenediol used; behavioral testing (locomotor activity; sexual behavior testing); teratology;"...this mode of administration was chosen instead of repeated injections or oral gavage to pups, in order to minimize stress. In addition, minipumps have successfully been used during the postpartum period"pg125; effects of prenatal stress;.

13570 Zana-Taieb, E., Butruille, L., Franco-Montoya, M.L., Lopez, E., Vernier, F., Grandvuillemin, I., Evain-Brion, D., Deruelle, P., Baud, O., Delacourt, C., Jarreau, P.H. Effect of Two Models of Intrauterine Growth Restriction on Alveolarization in Rat Lungs: Morphometric and Gene Expression Analysis. PLoS One 2013; 8(-11-):U35-U45. >>> L-NAME; Saline; SC; Rat (pregnant); Controls received high protein diet; animal info (Female, Sprague Dawley); teratology.

13543 Miki, T., Hirai, H., Takahashi, T. Activity-Dependent Neurotrophin Signaling Underlies Developmental Switch of Ca(2+) Channel Subtypes Mediating Neurotransmitter Release. Journal of Neuroscience 2013; 33(-48-):18755-18763. >>> Tetrodotoxin; K252a; Saline; CSF/CNS (cerebellum); Rat (neonate); 1007D; Controls received mp w/ vehicle; animal info (Wistar/ST, Postnatal day 6); ALZET brain infusion kit 1 used; tissue perfusion (cerebellum); teratology; good methods(pg. 18757); Pump implantation shown figure 6A pg.P18761. K252a is a CaM kinase and phosphorylase inhibitor;.

13371 Feng, X.D., Reini, S.A., Richards, E., Wood, C.E., Keller-Wood, M. Cortisol stimulates proliferation and apoptosis in the late gestation fetal heart: differential effects of mineralocorticoid and glucocorticoid receptors. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2013; 305(-4-):R343-R350. >>> Cortisol; potassium canrenoate; mifepristone; Saline; SC; Sheep (ewe); 2ML2; Controls received mp w/ vehicle; animal info (female, singleton pregnancies); teratology; cardiovascular; impact of maternal stress during late gestation.

13283 Kundakovic, M., Champagne, F.A. Epigenetic perspective on the developmental effects of bisphenol A. BRAIN BEHAVIOR AND IMMUNITY 2011; 25(-6-):1084-1093. >>> Bisphenol A; Mice (pregnant); Animal info (pregnant and lactating CD-1); behavioral testing (sexual dimorphism); Prenatal exposure to BPA, even at much lower reference levels, have found to be detrimental to development (CNS development, neuroendocrine development); The effects may also be transmitted across several generations through epigenetics; teratology;.

13191 Cao, J., Wang, J., Dwyer, J.B., Gautier, N.M., Wang, S., Leslie, F.M., Li, M.D. Gestational nicotine exposure modifies myelin gene expression in the brains of adolescent rats with sex differences. TRANSLATIONAL PSYCHIATRY 2013; 3(-;-):U33-U42. >>> Nicotine; SC; Rat (pregnant); 2002; Controls received mp w/ saline; dependence; animal info (Sprague Dawley, pregnant, delivery started from gestation day 4); teratology.

13174 Wang, H., Gondre-Lewis, M.C. Prenatal Nicotine and Maternal Deprivation Stress De-Regulate the Development of CA1, CA3, and Dentate Gyrus Neurons in Hippocampus of Infant Rats. PLoS One 2013; 8(-6-):U924-U939. >>> Nicotine hydrogen tartrate; Saline; SC; Mice (pregnant); 2004; 2006; Controls received mp w/ saline; animal info (pregnant dams); teratology.

13150 Acevedo, N., Davis, B., Schaeberle, C.M., Sonnenschein, C., Soto, A.M. Perinatally Administered Bisphenol A as a Potential Mammary Gland Carcinogen in Rats. Environmental Health Perspectives 2013; 121(-9-):1040-1046. >>> Bisphenol A; DMSO; SC; Rat (pregnant); 2002; 2004; Control animals received mp w/ vehicle; animal info (Sprague Dawley, female, virgin, 8-10 wks old); 50% DMSO used; dose-response; teratology.

13095 Kasberg, A.D., Brunskill, E.W., Potter, S.S. SP8 regulates signaling centers during craniofacial development. Developmental Biology 2013; 381(-2-):312-323. >>> Cyclopamine; Sodium citrate phosphate; cyclodextrin, hydroxypropyl beta; SC; Mice (pregnant); 2001D; 30% cyclodextrin used; multiple pumps used (2);"Due to the low penetrance of the rescue via IP injection... we used micro-osmotic pumps to provide a more effective method of delivering cyclopamine."pg 320; comparison of IP injections versus SC mp; teratology.

13088 Chang, G.Q., Karatayev, O., Leibowitz, S.F. Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala. Journal of Neuroscience 2013; 33(-34-):13600-13611. >>> Nicotine; Saline; SC; Rat (pregnant); 2002; Control animals received mp w/ vehicle; animal info (Sprague Dawley, female, 220-240 g); teratology.

13073 Li, Y., Xiao, D.L., Yang, S.M., Zhang, L.B. Promoter methylation represses AT(2)R gene and increases brain hypoxic-ischemic injury in neonatal rats. NEUROBIOLOGY OF DISEASE 2013; 60(-;-):32-38. >>> Nicotine; Saline; SC; Rat (pregnant); 2ML4; Control animals received mp w/ vehicle; animal info (Sprague Dawley, female); teratology.

12776 Jaiswal, S.J., Pilarski, J.Q., Harrison, C.M., Fregosi, R.F. Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-Botzinger Complex of Neonatal Rats. Journal of Neuroscience 2013; 33(-6-):2616-2625. >>> Nicotine bitartrate; Saline, physiological; SC; Rat (pregnant); Control animals received mp w/ vehicle; animal info (Sprague Dawley, female, GD5); teratology.

12587 Nishijima, K., Shukunami, K., Yoshinari, H., Takahashi, J., Maeda, H., Takagi, h., Kotsuji, F. Interactions among pulmonary surfactant, vernix caseosa, and intestinal enterocytes: intra-amniotic administration of fluorescently liposomes to pregnant rabbits. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 2012; 303(-3-):L208-L214. >>> Liposomes, fluorescently labeled; coatasome EL-01-C, hydrated; DMSO; water, distilled; Intrauterine; Rabbit (fetus); 2ML1; Control animals received mp w/ liposome alone; animal info (Japanese, White, 4.2-5.4 kg, teen); tissue perfusion (fetus);"5-cm sterile PE 60 silicone catheter with silicone flange was attached to each pump"pg L209; Fig 2, image of pump and catheter placement; multiple pumps used (2); teratology.

12561 McCarthy, F.P., Drewlo, S., English, F.A., Kingdom, J., Johns, E.J., Kenny, L.C., Walsh, S.K. Evidence Implicating Peroxisome Proliferator-Activated Receptor-gamma in the Pathogenesis of Preeclampsia. Hypertension 2011; 58(-5-):882-U447. >>> T0070907; DMSO; IP; Rat (pregnant); 2ML1; Controls received mp w/ vehicle; animal info (Sprague Dawley, female); 40% DMSO used; T0070907 is a peroxisome proliferator-activated receptor gamma specific antagonist; teratology.

12451 Hu, H.X., Brahmbhatt, A., Upadhyaya, R., Vega, D., Hill, A.A. Prenatal nicotine exposure alters the response of the mouse in vitro respiratory rhythm to hypoxia. RESPIRATORY PHYSIOLOGY&NEUROBIOLOGY 2012; 181(-2-):234-247. >>> Nicotine hydrogen tartrate; Water, sterile; SC; Mice (pregnant); 2004; Controls received mp w/ vehicle; animal info (CD-1, G4); teratology; While this method is widely used in animals studies, one potential criticism is that, due to the continuous nature of nicotine release from an osmotic mini-pump, it is a poor mimic of episodic smoking in humans. Empirical evidence has shown, however, that smokers tend to have constant blood nicotine levels throughout the day... Additionally, the use of osmotic mini-pumps avoids periods of transient hypoxia-ischemia that occur with each nicotine injection... and allows for the delivery of a continuous fixed dose of nicotine that is very similar to the continuous nicotine delivery provided by transdermal patches in humans."pg 235.

12373 Butruille, L., Mayeur, S., Duparc, T., Knauf, C., Moitrot, E., Fajardy, I., Valet, P., Storme, L., Deruelle, P., Lesage, J. Prenatal fasudil exposure alleviates fetal growth but programs hyperphagia and overweight in the adult male rat. European Journal of Pharmacology 2012; 689(-1-3-):278-284. >>> L-NAME; fasudil; SC; Rat (pregnant); Controls received mp w/ normal saline; animal info (Wistar Han, 200-250 g, E14, female); enzyme inhibitor (nitric oxide synthase, NOS, Rho-kinase, ROCK); one group contained mixture of fasudil and L-NAME; teratology.

12199 Pilarski, J.Q., Wakefield, H.E., Fuglevand, A.J., Levine, R.B., Fregosi, R.F. Increased nicotinic receptor desensitization in hypoglossal motor neurons following chronic developmental nicotine exposure. Journal of Neurophysiology 2012; 107(-1-):257-264. >>> Nicotine; SC; Rat (pregnant); 1007D; Animal info (Sprague Dawley, female); teratology.

12128 Parameshwaran, K., Buabeid, M.A., Karuppagounder, S.S., Uthayathas, S., Thiruchelvam, K., Shonesy, B., Dityatev, A., Escobar, M.C., Dhanasekaran, M., Suppiramaniam, V. Developmental nicotine exposure induced alterations in behavior and glutamate receptor function in hippocampus. CELLULAR AND MOLECULAR LIFE SCIENCES 2012; 69(-5-):829-841. >>> Nicotine; SC; Rat (pregnant); 2004; Controls received mp w/ physiological saline;"Nicotine in rodents has a short half-life and therefore the use of an osmotic mini pump would ensure a steady infusion. This also fits the human condition, as amniotic fluid maintains a reservoir of steady levels of nicotine so that the fetus is continuously exposed."pg 837; half-life"short"pg 837; teratology.


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What researchers are saying...

“We administered nicotine to achieve a steady-state concentration, using subcutaneously implanted minipumps, a technique that avoids the episodic hypoxic-ischemic episodes that accompany nicotine injections, and that also sidesteps the inherent problems of repeated handling stress and episodic withdrawal between injections” Slotkin et al., Neurotoxicology and Teratology 2002; 24:369-384.