References on the Intratumoral Administration of Agents Using ALZET^® Osmotic Pumps

Q0783 Zhai,H.Y., Heppner,F.L., Tsirka,S.E. Microglia/Macrophages Promote Glioma Progression. Glia 2011; 59(-3-):472-485. >>> Ganciclovir; macrophage/microglia inhibitory factor; tuftsin; CSF/CNS (intratumoral); Mice; 14, 28 days; Negative controls received mp w/ saline; animal info (12-16 wks old, male, CD11b-HSVTK +/-, 25-30 g, C57BL/6); cancer (glioma); Plastics One guide cannula used; macrophage/microglia inhibitory factor also known as MIF/TKP is a tripeptide; tuftsin also known as threonine-lysine-proline-arginin or TKPR.

Q0781 Schulte,A., Guenther,H.S., Phillips,H.S., Kemming,D., Martens,T., Kharbanda,S., Soriano,R.H., Modrusan,Z., Zapf,S., Westphal,M., Lamszus,K. A Distinct Subset of Glioma Cell Lines with Stem Cell-like Properties Reflects the Transcriptional Phenotype of Glioblastomas and Overexpresses CXCR4 as Therapeutic Target. Glia 2011; 59(-4-):590-602. >>> AMD3100; CSF/CNS (intratumoral); Mice; 2004; ; Controls received mp w/ PBS; animal info (6-8 wks old, NMRI-nu/nu); ALZET brain infusion kit 2 used; cancer (glioma).

Q0773 Cuevas,P., Carceller,F., Angulo,J., Gonzalez-Corrochano,R., Cuevas-Bourdier,A., Gimenez-Gallego,G. Antiglioma effects of a new, low molecular mass, inhibitor of fibroblast growth factor. Neuroscience Letters 2011; 491(-1-):1-7. >>> Dobesilate; PBS; CSF/CNS (intratumoral); Rat; 2004; 27 days; Controls received mp w/ vehicle; cancer (glioma); Compound, also known as (2,5-dihydroxyphenyl-sulfonate or 2,5DHPS) is an FGF inhibitor; chemotherapeutic.

Q0707 Tange,Y., Miyazaki,M., Iwata,J., Aiko,Y., Sakamoto,S., Mori,K. Novel Antitumor Effect of Carboplatin Delivered by Intracerebral Microinfusion in a Rat Malignant Glioma Model. NEUROLOGIA MEDICO-CHIRURGICA 2009; 49(-12-):572-579. >>> Carboplatin; Glucose, D-; Evans blue; CSF/CNS (intratumoral); Rat; 2001; 3, 7 days; Animal info (male, F344/N Slc, Fischer 220-260 g); brain tissue distribution; ALZET brain infusion kit used; stability verified (4 weeks) pg 573; cancer (glioma); MRI; brain tissue distribution; "... continuous intracerebral microinfusion using the osmotic mini-pump can provide broader distribution of agents than growth of the brain tumor."; "intracerebral microinfusion can attain clinically favorable drug distribution from a single infusion point." pg 576.

Q0095 Schmidt,N.O., Ziu,M., Cargioli,T., Westphal,M., Giese,A., Black,P.M., Carroll,R.S. Inhibition of thromboxane synthase activity improves glioblastoma response to alkylation chemotherapy. Translational Oncology 2010; 3(-1-):43-49. >>> Furegrelate; PBS; CSF/CNS (intratumoral); Mice (nude); 2004; 4 weeks; Controls received mp w/ vehicle; tissue perfusion (tumor); functionality of mp verified by residual volume; stress/adverse reaction: (see pg. 46) "hemosiderin deposits"; cancer (glioma); ALZET brain infusion kit used; animal info (Swiss, 4-6 wks old).

P9944 Agrawal,A., Min,D.H., Singh,N., Zhu,H.H., Birjiniuk,A., von Maltzahn,G., Harris,T.J., Xing,D.Y., Woolfenden,S.D., Sharp,P.A., Charest,A., Bhatia,S. Functional Delivery of siRNA in Mice Using Dendriworms. ACS Nano 2009; 3(-9-):2495-2504. >>> RNA, small interfering; epidermal growth factor receptor; dendriworm, dye-labeled; RNA, small interfering, GFP, dendriworm, dye-labeled; CSF/CNS (intratumoral); Mice; 3, 7 days; Tissue perfusion (tumor); cancer (glioblastoma); incorrectly stated pump model 2007; Plastics One cannula used; animial info (Swiss Webster).

P9779 Ozawa,T., Gryaznov,S., Hu,L.J., Pongracz,K., Santos,R.A.S., Bollen,A.W., Lamborn,K.R., Deen,D.F. Antitumor effects of specific telomerase inhibitor GRN163 in human glioblastoma xenografts1. NEURO-ONCOLOGY 2004/7; (--):218-226. >>> GRN163, FITC-; CSF/CNS (intratumoral); Rat (nude); 1007D; 7, 14 days; Tissue perfusion (tumor); enzyme inhibitor (telomerase); cancer (glioma); animal info (6-8 wks old, male, athymic).

P9777 Giraud,S., Bessette,B., Boda,C., Lalloue,F., Petit,D., Mathonnet,M., Jauberteau,M.O. In vitro apoptotic induction of human glioblastoma cells by Fas ligand plus etoposide and in vivo antitumour activity of combined drugs in xenografted nude rats. INTERNATIONAL JOURNAL OF ONCOLOGY 2007; 30(--):273-281. >>> Fas ligand; etoposide; dexamethasone; CSF, artificial; CSF/CNS (intratumoral); Rat (nude); 1002; 15 days; Tissue perfusion (tumor); functionality of mp verified by residual volume; cancer (glioblastoma); animal info (female 2 months old, 159 g.).

P9768 Kang,G.S., Di Wang,X., Mohler,M.L., Kirichenko,O.V., Patil,R., Orr,W.E., Miller,D.D., Geisert,E.E. Effects, in an in-vivo model system, of 1,2,3,4-tetrahydroisoquinoline on glioma. Anti-cancer Drugs 2008; 19(-9-):859-870. >>> EDL-155; HBSS; CSF/CNS (intratumoral); Rat; 2001; 7 days; Tissue perfusion (tumor); comparison of IP injections vs. mp; cancer (glioma); animal info (male, Sprague Dawley, 250-350 g); EDL-155 also known as 1-(biphenyl-4-ylmethyl)-1,2,3,4-tetrahydroisoquinoline-6,7-diol.

P9680 Martens,T., Laabs,Y., Guenther,H.S., Kemming,D., Zhu,Z.P., Witte,L., Hagel,C., Westphal,M., Lamszus,K. Inhibition of glioblastoma growth in a highly invasive nude mouse model can be achieved by targeting epidermal growth factor receptor but not vascular endothelial growth factor receptor-2. Clinical Cancer Research 2008; 14(-17-):5447-5458. >>> Cetuximab; CSF/CNS (intratumoral); Mice (nude); 2004; ; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioblastoma); ALZET brain infusion kit 2 used; animal info (NMRI- nu/nu, 6-8 wks old); cetuximab is a monoclonal antibody against EGFR.

P9661 Miyake,J.A., Benadiba,M., Colquhoun,A. Gamma-linolenic acid inhibits both tumour cell cycle progression and angiogenesis in the orthotopic C6 glioma model through changes in VEGF, Flt1, ERK1/2, MMP2, cyclin D1, pRb, p53 and p27 protein expression. Lipids in Health and Disease 2009; 8(-;-):U1-U10. >>> Gamma linolenic acid; CSF, artificial; CSF/CNS (intratumoral); Rat; 2002; 14 days; Controls received mp w/vehicle; cancer (glioma); ALZET brain infusion kit used; animal info (adult, female, Wistar, 250-350).

P9618 Rousseau,J., Barth,R.F., Moeschberger,M.L., Elleaume,H. EFFICACY OF INTRACEREBRAL DELIVERY OF CARBOPLATIN IN COMBINATION WITH PHOTON IRRADIATION FOR TREATMENT OF F98 GLIOMA-BEARING RATS. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 2009; 73(-2-):530-536. >>> Carboplatin; Dextrose; CSF/CNS (intratumoral); Rat; 2001; 6 days; Controls received mp w/vehicle; no stress (see pg. 532); cancer (glioma); ALZET brain infusion kit 2 used; animal info (male, Fisher, 230-260g); "Surgery for pump implantation was well tolerated." pg. 532; "It is noteworthy that the brainstem was not damaged by direct infusion of 200 ul of carboplatin (0.5 mg/mL) at a flow rate of 1uL/h." pg. 535; tissue perfusion (tumor).

P9478 Schauff,A.K., Kim,E.L., Leppert,J., Nadrowitz,R., Wuestenberg,R., Brockmann,M.A., Giese,A. Inhibition of invasion-associated thromboxane synthase sensitizes experimental gliomas to gamma-radiation. Journal of Neuro-oncology 2009; 91(-3-):241-249. >>> Furegrelate; CSF/CNS (intratumoral); Mice (nude); 1002; 14 days; Controls were untreated; enzyme inhibitor (thromboxane synthase TXSA); cancer (glioma); tissue perfusion (tumor); ALZET brain infusion kit 3 used; animal info (NMRI).

P9206 Zagorac,D., Jakovcevic,D., Gebremedhin,D., Harder,D.R. Antiangiogenic effect of inhibitors of cytochrome P450 on rats with glioblastoma multiforme. Journal of Cerebral Blood Flow and Metabolism 2008; 28(-8-):1431-1439. >>> Miconazole; octadecynoic acid, 17-; Ethanol; CSF, artificial; CSF/CNS (intratumoral); Rat; 1002; 14 days; Controls received mp w/ vehicle; enzyme inhibitor (CYP epoxygenase); tissue perfusion (tumor); ALZET brain infusion kit used; animal info (male, Fisher, 8-10 wks old); 10% ethanol used; antiangiogenesis.

P9057 Masunaga,S., Sakurai,Y., Nagata,K., Suzuki,M., Maruhashi,A., Kinashi,Y., Nagasawa,H., Uto,Y., Hori,H., Ono,K. The usefulness of a continuous administration of tirapazamine combined with reduced dose-rate irradiation using gamma-rays or reactor thermal neutrons. British Journal of Radiology 2006; 79(-948-):991-998. >>> Uridine, bromodeoxy-; tirapazamine; Saline; SC; Mice; 2001D; 5 days; 24 hours; Tissue perfusion (tumor); comparison of IP injections vs. mp; cancer; animal info (8-11 wks old, female, syngenic C3H, He); tirapazamine, also known as TP2, is a lead compound as a bioreactive hypoxic cytotoxin; labing of P cells; cancer.

P8729 Masunaga,S., Takahashi,A., Ohnishi,K., Nagata,K., Suzuki,M., Kashino,G., Kinashi,Y., Ohnishi,T., Ono,K. Dependency of repair of radiation-induced damage on p53 status and efficient inhibition of the repair with wortmannin treatment in vivo, with reference to the effect on intratumor quiescent cell population. Oncology Reports 2007; 18(-6-):1589-1596. >>> Uridine, bromodeoxy-; Saline; SC; Mice (nude); 2001; 7 days; Tissue perfusion (tumor); cancer; animal info (Balb/cA, nude); labeling of tumor cells.

P8366 Dorrell,M.I., Aguilar,E., Scheppke,L., Barnett,F.H., Friedlander,M. Combination angiostatic therapy completely inhibits ocular and tumor angiogenesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-3-):967-972. >>> Vascular endothelial growth factor, aptamer; EMD472523; T2-TrpRS; PBS; CSF/CNS (intratumoral); Rat; 2001D; 2ML2; 2ML4; 3,6 days; 24 hours; Controls received mp w/ vehicle; cancer (gliosarcoma); animal info (male, Fischer, 344); EMD472523 is an integrin antagonist, VEGF aptamer is chemically identical to Macugen; T2 is a proteolytic fragment of tryptophan tRNA synthetase; all agents have angiostatic activity; tissue perfusion (tumor).

P8287 Manabe,T., Okino,H., Maeyama,R., Mizumoto,K., Tanaka,M., Matsuda,T. New infusion device for trans-tissue, sustained local delivery of anticancer agent to surgically resected tissue: Potential use for suppression of local recurrence of pancreatic cancer. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 2005; 73B(-1-):203-207. >>> Gemcitabine; SC; Mice (nude); 1007D; 7 days; Tissue perfusion (intratumoral); comparison of IP injections; cancer (pancreatic); animal info (five week old); pump used as part of a novel infusion device; pictures of device p. 205; PE tubing used to connect pump to perfusion device; continuous infusion resulted in macroscopically undetectable tumors after day 6.

P8208 Martens,T., Schmidt,N.O., Eckerich,C., Fillbrandt,R., Merchant,M., Schwall,R., Westphal,M., Lamszus,K. A novel one-armed anti-c-met antibody inhibits glioblastoma growth in vivo. Clinical Cancer Research 2006; 12(-20-):6144-6152. >>> Antibody, anti-c-Met, OA5D5; Histidine; CSF/CNS (intratumoral); Mice (nude); 2004; 12, 21, 28 days; Controls received mp w/ vehicle; cancer (glioblastoma); ALZET brain infusion kit 2 used; animal info (male, NMRI-nu/nu, 6-8 wks old); "Because the OA-5D5 antibody is a relatively large but stable molecule, convection-enhanced drug delivery (by mp) was ideally suited to deliver it in our orthotopic model." (p.6151); tissue perfusion (tumor).

P8145 Grommes,C., Landreth,G.E., Sastre,M., Beck,M., Feinstein,D.L., Jacobs,A.H., Schlegel,U., Heneka,M.T. Inhibition of in vivo glioma growth and invasion by peroxisome proliferator-activated receptor gamma agonist treatment. MOLECULAR PHARMACOLOGY 2006; 70(-5-):1524-1533. >>> Pioglitazone; DMSO; PBS; CSF/CNS (intratumoral); Rat; 2ML4; 3, 6, 9, 14, 21 days; Controls received mp w/ vehicle; comparison of oral admin. vs. mp; no stress (see p.1532); stability verified by in vitro incubation at 37 celsius for 21 days; cancer (glioma); ALZET brain infusion kit used; animal info (Sprague-Dawley, 200-250g.); 0.1% DMSO; Antineoplastic; tissue perfusion (tumor).

P7975 Pyrko,P., Wang,W., Markland,F.S., Swenson,S.D., Schmitmeier,S., Schonthal,A.H., Chen,T.C. The role of contortrostatin, a snake venom disintegrin, in the inhibition of tumor progression and prolongation of survival in a rodent glioma model. Journal of Neurosurgery 2005; 103(-3-):526-. >>> Contortrostatin; CSF/CNS (intratumoral); Mice; 2 weeks; Controls received mp w/ PBS; plasma levels taken; cancer (glioma); brain tissue distribution; snake venom disintegrin; "agent was well tolerated by animals and lacks obvious neurotoxic side effects. All the contortrostatin treated mice survived the entire treatment period and continued to do well for seven more days after the termination." p. 533; tygon tubing used; tissue perfusion (tumor).

P7905 Shir,A., Ogris,M., Wagner,E., Levitzki,A. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. PLOS MEDICINE 2006; 3(-1-):125-135. >>> RNA, double stranded; polyinosine-cytosine (poly IC) dsRNA; Non-viral vector; SC; Mice; 2001D; 2 days; Per communication with author on 8/11/07, "We did not have any particular problems with the anesthesia exposure. For the pump replacement procedure Ketamine/Xylazine 2% (0.85ml/0.15ml), 40-50 ul IP per mouse (25 gram) was used. Replacing of the pump was not problematic either. The skin was closed with a surgical clips which are less traumatic to the mice and are easily applied and removed." 2001D pump replaced every day for two days total duration; synthetic dsRNA was attached to a non-viral vector (PEI-PEG-EGF beta PEI-Mel complexes) with specificity to the epidermal growth factor receptor (EGFR); controls received vector only (no poly IC); tissue perfusion (intratumoral); cancer (glioblastoma, breast, adenocarcinoma).

P7904 Harding,T.C., Lalani,A.S., Roberts,B.N., Yendluri,S., Luan,B., Koprivnikar,K.E., Gonzalez-Edick,M., Guang,H.T., Musterer,R., VanRoey,M.J., Ozawa,T., LeCouter,R.A., Deen,D., Dickinson,P.J., Jooss,K. AAV serotype 8-mediated gene delivery of a soluble VEGF receptor to the CNS for the treatment of glioblastoma. MOLECULAR THERAPY 2006; 13(-5-):956-966. >>> Adeno-associated virus serotype 8 vector, recomb.; CSF/CNS (intratumoral); Rat; 2001D; 24 hours; Controls received mp w/ AAV control; cancer (glioblastoma multiforme, u-251 MG); gene thrapy; animal info (male, athymic, 6 wk. old); rAAV contains soluble VEGF inhibitor, sVEGFR1/R2; "Implantation of the mini pump allows the slow infusion of a rAAV vector...to transduce more effectively the intracranial tumor mass." (pg. 962); antiangiogenesis.

P7434 Kawakami,K., Kioi,M., Liu,Q., Kawakami,M., Puri,R.K. Evidence that IL-13R alpha-2 chain in human glioma cells is responsible for the antitumor activity mediated by receptor-directed cytotoxin therapy. Journal of Immunotherapy 2005; 28(-3-):193-202. >>> Interleukin-13-PE38; interleukin-13Ra2, pME18S-; CSF/CNS (intratumoral); Mice (nude); 1003D; 1007D; 7 days; Controls received mp w/ vehicle or antisense IL-13Ra2 plasmid vector; pumps replaced after 3 days; cancer (glioblastoma); cyanoacrylate adhesive; convection enhanced delivery; IL-13Ra2 cDNA encoding plasmid vector; "the upregulated IL-13 Ra2 chain was successfully targeted with a continuous infusion of IL-13 cytotoxin." (p. 199).

P7283 Ozawa,T., Afzal,J., Lamborn,K.R., Bollen,A.W., Bauer,W.F., Koo,M.S., Kahl,S.B., Deen,D.F. Toxicity, biodistribution, and convection-enhanced delivery of the boronated porphyrin BOPP in the 9L intracerebral rat glioma model. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 2005; 63(-1-):247-252. >>> Porphyrin, boronated; PBS; CSF/CNS (intratumoral); Rat; 2001D; 24 hours; Controls received no treatment; dose-response; comparison of IV injections vs. mp; cancer (brain glioma); toxicology.

P7197 MacKay,J.A., Deen,D.F., Szoka,FC Jr  Brain Research 2005; 1035(-2-):139-153. >>> Liposomes; FITC-dextran-lysine; Saline; tris buffer; CSF/CNS (caudate putamen); CSF/CNS (intratumoral); Rat; 2001D; 24 hours; Tissue perfusion (intratumoral); comparison of acute CSF/CNS injection vs. mp; half-life (p. 151) 9.9 hours; cancer (glioblastoma); ALZET brian infusion kit 2 used; brain tissue distribution; post op. care (buprenophine).

P7081 Matsuno,A., Katayama,H., Murakami,M., Nagashima,T. In vivo growth suppression of rat C6 glioma transplanted in rat brain using antisense oligonucleotide for microtubule-associated protein 1A messenger ribonucleic acid. British Journal of Neurosurgery 2004; 18(-4-):343-346. >>> Oligodeoxynucleotide, phosphorothisate antisense; oligodeoxynucleotide, phosphorothisate sense; oligodeoxynucleotide, phosphorothisate scramble; Saline, sterile; CSF/CNS (intratumoral); Rat; 1007D; 1 week; Controls received mp w/ sense or scrambled oligos; antisense (microtubule-associated protein 1A); cancer (glioma).

P7077 Lefranc,F., Mijatovic,T., Mathieu,V., Rorive,S., Decaestecker,C., Debeir,O., Brotchi,J., Van Ham,P., Salmon,I., Kiss,R. Characterization of gastrin-induced proangiogenic effects in vivo in orthotopic U373 experimental human glioblastomas and in vitro in human umbilical vein endothelial cells. Clinical Cancer Research 2004; 10(-24-):8250-8265. >>> Gastrin-17; Saline; CSF/CNS (intratumoral); Rat (nude); 1002; 7 days; Controls received mp w/ vehicle; stability verified; cancer (glioma).

P6775 Gawlick,U., Kranz,D.M., Schepkin,V.D., Roy,E.J. A conjugate of a tumor-targeting ligand and a T cell costimulatory antibody to treat brain tumors. Bioconjugate Chemistry 2004; 15(-5-):1137-1145. >>> Antibody, anti-CD28 Fab/Folate; gadolinium; PBS; CSF/CNS (intratumoral); Mice (transgenic); 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by MRI w/ gadolinium infusion; comparison of IV, SC, ICV injections vs. mp; cancer (brain, choroid plexus); brain tissue distribution; MRI; cyanoacrylate adhesive; "The strongest effect on tumor growth assessed of anti-CD28 fab/Folate." (p. 1143). Plastics One cannula used.

P6764 Frewert,S., Stockhammer,F., Warschewske,G., Zenclussen,A.C., Rupprecht,S., Volk,H.D., Woiciechowsky,C. Intratumoral infusion of interleukin-1 beta and interferon-gamma; induces tumor invasion with macrophages and lymphocytes in a rat glioma model. Neuroscience Letters 2004; 364(-3-):145-148. >>> Interleukin-1, beta recomb. rat; Interferon-gamma, recomb. rat; Saline, physiological; albumin, human serum; CSF/CNS (intratumoral); Rat; 1003D; 48 hours; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioma).

P6720 Gondi,C.S., Lakka,S.S., Dinh,D.H., Olivero,W.C., Gujrati,M., Rao,J.S., Rao,J.S. RNAi-mediated inhibition of cathepsin B and uPAR leads to decreased cell invasion, angiogenesis and tumor growth in gliomas. ONCOGENE 2004; 23(--):8486-8496. >>> RNA, small interfering; EV/SV vector; PBS; CSF/CNS (intratumoral); Mice (nude); ; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioma); gene therapy; antiangiogenesis; siRNA (cathepsin B); pump model not stated (0.25 ul/hr); "...local intracranial delivery of pcu using mini-osmotic pumps effectively inhibited human malignant glioma growth.".

P6557 Chi,K.H., Wang,H.E., Wang,Y.S., Chou,S.L., Yu,H.M., Tseng,Y.H., Hwang,I.M., Lui,W.Y. Antisense thymidylate synthase electrogene transfer to increase uptake of radiolabeled iododeoxyuridine in a murine model. Journal of Nuclear Medicine 2004; 45(-3-):478-484. >>> Uridine; iodoeoxy; radio-isotopes; Saline, normal; 131I tracer; Intratumoral; Mice; 2001; 7 days; Controls received mp w/ PBS; cancer; "The present study was designed to increase the extent of cellular uptake of 131I-IdUrd in experimental tumors by using a miniosmotic pump." (p. 483).

P6554 Hjortland,G.O., Garman-Vik,S.S., Juell,S., Olsen,O.E., Hirschberg,H., Fodstad,O., Engebraaten,O. Immunotoxin treatment targeted to the high-molecular-weight melanoma-associated antigen prolonging the survival of immunodeficient rats with invasive intracranial human glioblastoma multiforme. Journal of Neurosurgery 2004; 100(-2-):320-327. >>> Antibody, monoclonal 9.2.27 - pseudomonas exotoxin A; dye, evans blue; PBS; CSF/CNS (intratumoral); Rat (nude); 2001D; 24 hours; Controls received mp w/ PBS; cancer (glioma biopsy tumors); ALZET brain infusion kit used; brain tissue distribution; mp primed in sterile saline at 37 degrees Celsius for 4 hours; mp removed after 3 days.

P6540 Grossi,P.M., Ochiai,H., Archer,G.E., McLendon,R.E., Zalutsky,M.R., Friedman,A.H., Friedman,H.S., Bigner,D.D., Sampson,J.H. Efficacy of intracerebral microinfusion of trastuzumab in an Athymic rat model of intracerebral metastatic breast cancer. Clinical Cancer Research 2003; 9(-15-):5514-5520. >>> Herceptin; antibody, anti-Her2 monoclonal; radio-isotopes; Saline; 125I tracer; IP; CSF/CNS (intratumoral); Rat (athymic); 2ML1; 7 days; Tissue perfusion (tumor); herceptin serum levels (p. 5516); comparison of systemic IP mp vs. intracerebral mp infusion; cancer (breast); brain tissue distribution; radiolabeled herceptin (transtuzumab) was also infused intratumorally, peritumorally, and i.p to study tissue distribution.

P6535 Fischer,W., Gustafsson,L., Mossberg,A.K., Gronli,J., Mork,S., Bjerkvig,R., Svanborg,C. Human alpha-lactalbumin made lethal to tumor cells (HAMLET) kills human glioblastoma cells in brain xenografts by an apoptosis-like mechanism and prolongs survival. Cancer Research 2004; 64(-6-):2105-2112. >>> Lactalbumin, alpha; HAMLET; radio-isotopes; 125I tracer; saline; CSF/CNS (intratumoral); Rat (nude); 2001D; 1 day; Tissue perfusion (tumor); functionality of mp verified by autoradiography on brain sections; no stress (see pg. 2108); cancer (glioblastoma); brain tissue distribution; agent is called HAMLET or (human alpha lact albumin made lethal to tumor cells); "...there were no signs of edema or tissue damage in the surrounding brain, including the cortex, which had been penetrated by the [26 gauge] infusion cannula." p. 2108.

P6265 Tepel,J., Kruse,M.L., March,C., Fiedler,A., Kapischke,M., Ketterer,T., Sipos,B., Kremer,B., Kalthoff,H. Terminally modified oligodeoxynucleotides directed against p53 in an orthotopic xenograft model: A novel adjuvant treatment strategy for pancreatic ductal carcinoma. Pancreas 2004; 28(-1-):1-12. >>> Oligodeoxynucleotide, antisense; SC; Mice (SCID); 14 days; Tissue perfusion (intratumoral); comparison of IP injections vs. sc mp; cancer (pancreatic ductal carcinoma); transplantation; 200 ul pump used.

P6148 Leaver,H.A., Wharton,S.B., Bell,H.S., Leaver-Yap,I.M.M., Whittle,I.R. Highly unsaturated fatty acid induced tumor regression in glioma pharm acodynamics and bioavailability of gamma linolenic acid in an implanta tion glioma model: effects on tumour biomass, apoptosis and neuronal t issue histology. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS 2002; 67(-5-):283-292. >>> Linolenic acid, gamma; PBS; CSF/CNS (intratumoral); 2001; 3-7 days; Controls received mp w/ vehicle; tissue perfusion (tumor); cancer (glioma); ALZET brain infusion kit used.

P5991 Rubin,J.B., Kung,A.L., Klein,R.S., Chan,J.A., Sun,Y.P., Schmidt,K., Kieran,M.W., Luster,A.D., Segal,R.A. A small-molecule antagonist of CXCR4 inhibits intracranial growth of primary brain tumors. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2003; 100(-23-):13513-13518. >>> AMD 3100; PBS; SC; Mice; 21 days; Controls received mp w/ vehicle; tissue perfusion (intratumoral); comparison of 2x daily injections vs. mp; cancer (glioblastoma); CXCR4 antagonist; 0.5 ul/hr pumps used; In vivo tumor imaging performed with IVIS imaging system (Xenogen); bioluminescence imaging (BLI).

P5950 Schmidt,N.O., Ziu,M., Carrabba,G., Giussani,C., Bello,L., Sun,Y.P., Schmidt,K., Albert,M., Black,P.M., Carroll,R.S. Antiangiogenic therapy by local intracerebral microinfusion improves treatment efficiency and survival in an orthotopic human glioblastoma model. Clinical Cancer Research 2004; 10(-4-):1255-1262. >>> Endostatin, recomb. murine; PBS; CSF/CNS (intratumoral); Mice (nude); 2004; 21 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; comparison of SC injections vs. chronic ICV mp; no stress (see pg. 1261); cancer (glioma); ALZET brain infusion kit 1 used (per Dr. Carroll); MRI; "The direct infusion of therapeutic compounds into brain tumors can overcome some of the obstacles of drug delivery." p. 1255; BIK's removed prior to MRI; "no signs of intracranial bleeding due to the presence of the pumps or antiangiogenic treatment were observed..." (p. 1261); angiogenesis inhibitor; surgical glue used to affix cannula to the skull.

P5835 Svechnikova,I., Gray,S.G., Kundrotiene,J., Ponthan,F., Kogner,P., Ekstrom,T.J. Apoptosis and tumor remission in liver tumor xenografts by 4-phenylbutyrate. INTERNATIONAL JOURNAL OF ONCOLOGY 2003; 22(-3-):579-588. >>> Platelet factor-4; MMP-2 (hemopexin fragment); PBS; SC; CSF/CNS (intratumoral); Mice; 2004; 7 days; Controls received mp w/ PBS; tissue perfusion (tumor); dose-response (p.2502); comparison of IP injections SC systemic mp infusion vs. local intracranial mp infusion; long-term study; pumps replaced after 28 days in some groups; stability verified by in vitro assay; cancer (glioma); Angiogenesis inhibitors; COOH terminal fragment of PF-4 used; In vitro activity of agents confirmed for up to 16 days; "In conclusion, our data demonstrates that local intracerebral delivery of endogenous inhibitors by osmotic minipumps is a very effective modality for the treatment of aggressive tumors..." (p.2505); tissue perfusion (tumor);.

P5754 Galve-Roperh,I., Sanchez,C., Cortes,M.L., Gomez Del Pulgar,T., Izquierdo,M., Guzman,M. Anti-tumoral action of cannabinoids: involvement of sustained ceramide accumulation and extracellular signal-regulated kinase activation. Nat Med 2000; 6(-3-):313-319. >>> WIN-55,212-2; Cannabinol, delta-9-tetrahydro-; PBS; BSA; CSF/CNS (intratumoral); Rat; 2001; 7 days; Tissue perfusion (tumor); cancer; WIN-55,212-2 is a potent synthetic cannabinol agonist.

P5664 Hagedorn,M., Zilberberg,L., Wilting,J., Canron,X., Carrabba,G., Giussani,C., Pluderi,M., Bello,L., Bikfalvi,A. Domain swapping in a COOH-terminal fragment of platelet factor 4 generates potent angiogenesis inhibitors. Cancer Research 2002; 62(-23-):6884-6890. >>> Platelet factor 4 peptides; PBS; CSF/CNS (intratumoral); Mice (nude); 2004; 30 days; Controls received mp w/ vehicle; tissue perfusion (intratumoral); dose-response (p. 6888; fig 6); cancer; peptides; glioma; antiangiogenesis..

P5587 Lefranc,F., Camby,I., Belot,N., Bruyneel,E., Chaboteaux,C., Brotchi,J., Mareel,M., Salmon,I., Kiss,R. Gastrin significantly modifies the migratory abilities of experimental glioma cells. Lab.Invest. 2002; 82(-9-):1241-1252. >>> Gastrin; SC; rat; 1002; 14 days; controls received mp with saline; tissue perfusion (tumor resection cavity); cancer.

P5531 Chen,Y., Lin,S.M., Lai,H.S., Tseng,S.H., Chen,W.J. Effects of irradiated tumor vaccine and continuous localized infusion of granulocyte-macrophage colony-stimulating factor on neuroblastomas in mice. Journal of Pediatric Surgery 2002; 37(-9-):1298-1304. >>> Colony-stimulating factor, GM; PBS; BSA; SC (tumor vaccine injection site); Mice; 1002; 14 days; Controls received mp w/ PBS; immunology; GM-CSF is recombinant murine; tissue perfusion.

P5489 Cuevas,P., Carceller,F., Diaz-Gonzalez,D., Cuevas,B., Fernandez,A., Garcia-Gomez,I., Dujovny,M. Inhibition of rat glioma growth by neomycin. Preliminary report. NEUROLOGICAL RESEARCH 2002; 24(-6-):522-524. >>> TIMP-1; TIMP-2; PBS; CSF/CNS (intratumoral); Rat; 2002; 26 days; Controls received mp w/ vehicle; tissue perfusion (tumor); functionality of mp verified by immunohistochemistry of agents; cancer; enzyme inhibitor; ALZET brain infusion kit used; MMP inhibitors (tissue inhibitors of metalloprotease = TIMP); rat glioma model.

P5357 Walker,T.L., Dass,C.R., Burton,M.A. Enhanced In Vivo tumour response from combination of carboplatin and low-dose c-myc antisense oligonucleotides. Anticancer Research 2002; 22(-4-):2237-2245. >>> Fibroblast growth factor, basic; Saline; IV (jugular); Intratumoral; Rat; 2004; 86 hours; Controls received mp w/ vehicle; tissue perfusion (intratumoral); cancer; peptides; bFGF delivered either by systemic IV infusion or intratumorally.

P5059 Davies,M.M., Burke,D., Carnochan,P., Glover,C., Kaur,S., Allen-Mersh,T.G. Basic fibroblast growth factor infusion increases tumour vascularity, blood flow and chemotherapy uptake. Acta Oncologica 2002; 41(-1-):84-90. >>> Butyrate, sodium;; CSF/CNS (intratumoral);; rat;; 2ML2;; 10, 14, or 28 days; Controls received w/ saline; tissue perfusion (tumor); functionality of mp verified by residual volume; dose-response (graph on p. 621); cancer (glioma); Butyrate, sodium is a naturally occurring four-carbon fatty acid;.

P4815 Hautmann,S., Huland,E., Wullbrand,A., Friedrich,M., Huland,H. Treatment of metastatic hormone-refractory prostate adenocarcinoma (MatLyLu) in Copenhagen rats with micro-osmotic interleukin-2 pumps.. Anticancer Research 2000; 20(--):4495-4498. >>> Interleukin-2;; Albumin, human;; SC; Peritumoral (orthotopic); rat;; 2002;; ; Controls received mp w/ vehicle; functionality of mp verified by in vitro assay; no stress (see p. 4496); good methods pumps weighed p. 4496; cancer (prostate); immunology; peptides; rats had a prostatic adenocarcinoma tumor implanted; Albumin vehicle was 20% concentration; SC&peritumoral orthotopic implantation; Note: these pumps were left in for 28 days;.

P4478 Redgate,E.S., Grudziak,A.G., Deutsch,M., Boggs,S.S. Enhanced uptake of [3H] spermidine by 9L rat brain tumors after direct intratumoral infusion of inhibitors of enzymes of the polyamine biosynthetic pathway.. Journal of Neuro-oncology 1999; 42(--):123-130. >>> Spermidine; Putrescine; Ornithine, difluoromethyl-;; Radio-isotopes; 3H tracer; CSF;; CSF/CNS;; rat;; 14 days;; Controls received mp w/vehicle; tissue perfusion (intratumoral); cancer; polyamines; delayed delivery for 3 days.

P4283 Pollina,J., Plunkett,R.J., Ciesielski,M.J., Lis,A., Barone,T.A., Greenberg,S.J., Fenstermaker,R.A. Intratumoral infusion of topotecan prolongs survival in the nude rat intracranial U87 human glioma model. Journal of Neuro-oncology 1998; 39(--):217-225. >>> Topotecan;; Saline;; CSF/CNS (glioma);; rat (nude);; 1003D;; 10 hours;; controls received mp w/ vehicle or no treatment; tissue perfusion (tumor); functionality of mp verified by examining pumps to see if they were empty; no stress (p. 220); cancer; peptides; topotecan is a DNA topoisomerase I inhibitor;.

P4112 Hautmann,S., Huland,E., Wulbrand,A., Friedrich,M., Huland,H. Treatment of metastatic hormone refractory adenocarcinoma of the prostate (mat ly ly) with micro-osmotic interleukin-2 pump in male copenhagen rats. Eur.Urol. 1998; 34(--):265-266. >>> Interleukin-2; Albumin; intratumoral; rat; no duration posted; controls received mp w/albumin; tissue perfusion (intratumoral); cancer (prostate); immunology; peptides.

P3860 Zhu,J., Zhang,L., Hanisch,U.K., Felgner,P.L., Reszka,R. A continuous intracerebral gene delivery system for in vivo liposome-mediated gene therapy. Gene Therapy 1996; 3(--):472-476. >>> Liposomes; Gene, herpes simplex virus thymidine kinase; Gene, lacZ; CSF/CNS (caudate nucleus); Rat; 1003D; 3 days; controls received mp w/LacZ gene; tissue perfusion (tumor); functionality of mp verified by gene expression; comparison of intracerebral injections vs. mp; no stress (see pg.473); stability verified by gene expression; ALZET brain infusion kit used; cancer; gene therapy; "DNA-liposome complexes were stable within minipumps at body temperature (37C) for 1-3 days." (pg.474); "continuous administration of DNA-liposome complexes did not result in significant in vivo toxicity." (pg.474).

P3783 Tsuchida,E., Urano,M. The effect of UCN-01 (7-hydroxystaurosporine), a potent inhibitor of protein kinase C, on fractionated radiotherapy or daily chemotherapy of a murine fibrosarcoma. Int.J.Radiat.Oncol.Biol.Phys. 1997; 39(-5-):1153-1161. >>> UCN-01; Citric acid; intratumoral; mice; 2001; 7 days; tissue perfusion (tumor); cancer; enzyme inhibitor.

P3780 Redgate,E.S., Grudziak,A.G., Deutsch,M., Boggs,S.S. Difluoromethylornithine enhanced uptake of tritiated putrescine in 9L rat brain tumors. Int.J.Radiat.Oncol.Biol.Phys. 1997; 38(-1-):169-174. >>> Ornithine, difluoromethyl-; Putrescine; CSF, artificial;; IP; CSF/CNS (corpus striatum); rat; 2001; 2002; no duration posted; tissue perfusion (tumor); functionality of mp verified by tumor radioactivity during infusion; stability verified (pg. 173); good methods (pp. 170-171); cancer; catheter used to delay drug infusion for 1 or 3 days after surgery.

P3523 Viola,J.J., Agbaria,R., Walbridge,S., Oshiro,E.M., Johns,D.G., Kelley,J.A., Oldfield,E.H., Ram,Z. In situ cyclopentenyl cytosine infusion for the treatment of experimental brain tumors. Cancer Res. 1995; 55(--):1306-1309. >>> Cyclopentenyl cytosine; Saline, normal; IP; intratumoral; rat; 2ML2; 2ML4; 6, 28 days; controls received mp w/ saline; tissue perfusion (tumor); cancer; ALZET brain infusion kit used; SC pump connected to IP catheter.

P2912 Okuda,T., Onda,M., Tokunaga,A., Sugisaki,Y. Stimulatory effect of EGF and inhibitory effect of sialoadenectomy on growth of an EGF receptor-hyperproducing human gastric cancer xenograft in nude mice. Jpn.J.Surg. 1994; 24(--):725-733. >>> Epidermal growth factor; PBS; SC; mice (nude); 2002; no duration posted; controls received mp with PBS; dose response; cancer; peptides; tissue perfusion (tumor); human EGF used.

P2525 Moses,B.L., Chan,D.W., Hruban,R.H., Forastiere,A., Richtsmeier,W.J. Comparison of intra-arterial and intravenous infusion of cisplatin for head and neck squamous cell carcinoma in a modified rat model. Arch Otolaryngol.Head Neck Surg. 1993; 119(--):612-617. >>> Cisplatin; IV (saphenous); IA (saphenous); rat (nude); 2ML1; 6 days; tissue perfusion (tumor xenograft); cancer.

P2481 Ikeda,Y., Carson,B.S., Lauer,J.A., Long,D.M. Therapeutic effects of local delivery of dexamethasone on experimental brain tumors and peritumoral brain edema. J.Neurosurg. 1993; 79(--):716-721. >>> Dexamethasone; intratumoral; rabbit; 6, 10 days; controls received no treatment; tissue perfusion (tumor); comparison of injections vs. mp; local vs. systemic treatment, graph pg. 719.

P2091 Riemenschneider,Th., Ruf,C., Kratzsch,H.C., Ziegler,M., Spath,G. Arterial, portal or combined arterio-portal regional chemotherapy in experimental liver tumours. J.Cancer Res.Clin.Oncol. 1992; 118(--):597-600. >>> Uridine, fluorodeoxy-; Heparin; Saline; IA (hepatic); IV (hepatic portal); rat; 2ML2; 7 days; tissue perfusion (tumor); cancer; hepatic artery accessed via gastroduodenal artery; portal vein via ileocolic vein; animal info (SD, F, 120-150 g).

P1707 Kimler,B.F., Martin,D.F., Evans,R.G., Morantz,R.A., Vats,T.S. Combination of radiation therapy and intracranial bleomycin in the 9L rat brain tumor model. Int.J.Radiat.Oncol.Biol.Phys. 1990; 18(--):1115-1121. >>> Bleomycin; CSF/CNS (intratumoral); cattle; rat; 2001; 2002; 7, 14 days; Antibiotic; controls received pumps with saline only; tissue perfusion (tumor); dose-response (graph, p. 1117); comparison of ip injections vs. mp.

P1470 Bouvier,G., Penn,R.D., Kroin,J.S., Beique,R.A., Guerard,M.-J., Lesage,J. Intratumoral chemotherapy with multiple sources. Ann.N.Y.Acad.Sci. 1989; 531(--):212-214. >>> Cisplatin; CSF/CNS (intratumoral); human; 2002; no duration posted; mp connected to multiple microcatheters and cannulae; tissue perfusion (tumor); cancer/immunology.

P0406 Penn,R.D., Kroin,J.S., Harris,J.E., Chiu,K.M., Braun,D.P. Chronic intratumoral chemotherapy of a rat tumor with cisplatin and fluorouracil. Appl.Neurophysiol. 1983; 46(--):240-244. >>> Fluorouracil; Cisplatin; Saline; CSF/CNS (cerebellum); rat; 2001; 1 week; comparison of agents effects; tissue perfusion (intratumoral); cancer chemotherapy.