References on the Administration of siRNA Using ALZET® Osmotic Pumps

16338 Figueroa, J. D., Serrano-Illan, M., Licero, J., Cordero, K., Miranda, J. D., De Leon, M. Fatty Acid Binding Protein 5 Modulates Docosahexaenoic Acid-Induced Recovery in Rats Undergoing Spinal Cord Injury. J Neurotrauma 2016; 33(-15-):1436-49. >>> Oligonucleotides, scramble nontargeting; siRNA; Ethanol; Intrathecal; Rat; 2001; Controls received mp w/ vehicle; animal info (Young adult female Sprague-dawley rats); good methods (pg 1443); spinal cord injury;“Pumps were primed with DHA-albumin complex (DHA), FABP5 siRNA, and vehicle controls” (pg 1443); ALZET rat intrathecal catheter used;.

16131 Gujrati, M., Vaidya, A., Lu, Z. R. Multifunctional pH-Sensitive Amino Lipids for siRNA Delivery. Bioconjug Chem 2016; 27(-1-):19-35. >>> RNA, small interfering/EHCO; PEGylated EHCO; Mice (nude); Controls received treated with nonspecific PEGylated EHCO/siGFP nanoparticles (PEGGFP) and non-PEGylated EHCO/HIF-1ǝ; cancer; gene therapy, RNA nanoparticle infusion; peptides;“These results indicate that PEGylation can significantly improve the stability of EHCO/siRNA nanoparticles during storage in solution, possibly by preventing the aggregation of the nanoparticles and providing better protection to the siRNA cargo from degradation” (pg 31);.

15979 Kim, Y. J., Kang, T. C. The role of TRPC6 in seizure susceptibility and seizure-related neuronal damage in the rat dentate gyrus. Neuroscience 2015; 307(--):215-30. >>> RNA, small interfering TRPC6; CSF/CNS; Rat; 1007D; Controls received mp w/ saline; animal info (male, Sprague Dawley, 7 weeks old); ALZET brain infusion kit 1 used; used dental cemement;.

15923 Oladosu, F. A., Conrad, M. S., O'Buckley, S. C., Rashid, N. U., Slade, G. D., Nackley, A. G. Mu Opioid Splice Variant MOR-1K Contributes to the Development of Opioid-Induced Hyperalgesia. PLoS One 2015; 10(-8-):e0135711. >>> RNA, small interfering 13 antisense; CSF, artifical; CSF/CNS (intrathecal); Mice; Controls received mp w/ sense siRNA; animal info (C57BL6J or CXB7/ByJ, 8-12 weeks old, 20-30g);.

15869 Ewe, A., Schaper, A., Barnert, S., Schubert, R., Temme, A., Bakowsky, U., Aigner, A. Storage stability of optimal liposome-polyethylenimine complexes (lipopolyplexes) for DNA or siRNA delivery. Acta Biomater 2014; 10(-6-):2663-73. >>> DNA, siRNA; Lipopolyplexes, (PEI F25-LMW, DPPC); SC; CSF/CNS; IP; cancer (mamma carcinoma, prostate carcinoma, colon carcinoma); PEI F25-LMW aka Polyethylenimine F-25 Low molecular weight; DPPC aka dipalmitoyl-phosphatidyl-choline;.

15837 Renjun Wang, PhD; Qian Huang, MD; Rui Zhou, BSc; Zengxiang Dong, PhD;, Yunfeng Qi, PhD; Hua Li, MS; Xiaowei Wei, MS; Hui Wu, MS; Huiping Wang, PhD;, Christopher S. Wilcox, MD, PhD; Michael Hultström, MD, PhD; Xiaofu Zhou, PhD;, En Yin Lai, MD, PhD  Circ Heart Fail. 2016; 9(--):1-10. >>> AntagomiR-7b; RNA, small interfering GABBR1; angiotensin II; CSF/CNS (paraventricular nucleus); Rat; 1004; 1002; animal info (male, Wistar, 180-200g); pumps replaced after 4 weeks; bilateral cannula used; tissue perfusion (paraventricular nucleus); cardiovascular; peptides; bilateral infusion; Dose (AntagomiR-7b or Ad-siGABBR1 40 ng/h; antiotensin II 1 ng/kg/min);.

15750 Kyoung Joo Cho,, So Young Cheon, &Gyung Whan Kim  SCIENTIFIC REPORTS 2016; 6(--):1-11. >>> 3-nitropropionic acid; RNA, small interfering ASK1; Transfection reagent; SC; CSF/CNS (striatum); Mice (transgenic); 1007D; Controls received mp w/ vehicle; animal info (male, C57BL6J or R6/2 HD tg, 10 weeks old); behavioral testing (rotarod apparatus);.

15284 Alexander Ewe a, Andreas Schaper b, Sabine Barnert c, Rolf Schubert c, Achim Temmed, Udo Bakowsky e,, Achim Aigner  Acta Biomateriala 2014; (-10-):2663-2673. >>> DNA, siRNA; Lipopolyplexes, (PEI F25-LMW, DPPC); SC; cancer (mamma carcinoma, prostate carcinoma, colon carcinoma); PEI F25-LMW aka Polyethylenimine F-25 Low molecular weight; DPPC aka dipalmitoyl-phosphatidyl-choline.

14909 Cuevas, S., Yang, Y., Konkalmatt, P., Asico, L.D., Feranil, J., Jones, J., Villar, V.A., Armando, I., Jose, P.A., Cuevas, S. Role of Nuclear Factor Erythroid 2-Related Factor 2 in the Oxidative Stress-Dependent Hypertension Associated With the Depletion of DJ-1. HYPERTENSION 2015; 65(--):1251-U173. >>> RNA, small interfering Drd2-; RNA, small interfering DJ-1-; TransIT; Kidney (subcapsular space); Mice; 1007D; Controls received mp w/ non-silencing RNA; animal info (male, C57BL6J, uninephrectomized); used PE tubing #0007701 with surgical glue; pump sutured to abdominal wall;.

14515 Yu, C.J., Liu, W., Chen, H.Y., Wang, L., Zhang, Z.R., Zhang, Z.R. BACE1 RNA interference improves spatial memory and attenuates Abeta burden in a streptozotocin-induced tau hyperphosphorylated rat model. Cell Biochemistry and Function 2014; 32(--):590-596. >>> Okadaic acid; RNA, small interfering; SC; Rat; 1004; Controls received mp w/ saline; animal info (Sprague Dawley, 11-12.5 months); pumps replaced every 4 weeks; neurodegenerative (Alzheimer's disease); behavioral testing (morris water maze); long-term study;.

14477 Weissmann, L., Quaresma, P.G.F., Santos, A.C., de Matos, A.H.B., Pascoal, V.D.B., Zanotto, T.M., Castro, G., Guadagnini, D., da Silva, J.M., Velloso, L.A., Bittencourt, J.C., Lopes-Cendes, I., Saad, M.J.A., Prada, P.O., Prada, P.O. IKKepsilon Is Key to Induction of Insulin Resistance in the Hypothalamus, and Its Inhibition Reverses Obesity. Diabetes 2014; 63(--):3334-3345. >>> RNA, small interfering; CSF/CNS; Mice; 1007D; Controls received mp w/ scrambled RNA; animal info (male, C57BL6J or db/db, 8 weeks old); diabetes; obesity; used Plastics One cannula;.

14429 Tan, M.S., Tan, L., Jiang, T., Zhu, X.C., Wang, H.F., Jia, C.D., Yu, J.T., Tan, L. Amyloid-beta induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer's disease. Cell Death&Disease 2014; 5(--):U228-U239. >>> RNA, small interfering NLRP1; RNA, small interfering, NLRP3; RNA, small interfering caspase-1; CSF, artificial; water, RNase-free; CSF/CNS (dorsal third ventricle); Mice (transgenic); Controls received mp w/ vehicle or control RNA; animal info (male, APP/PS1); functionality of mp verified by knowckdown of targetd gene; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer's disease); no stress"This dose of NLRP1 siRNA, caspase-1 siRNA or NLRP3 siRNA infusion was well tolerated, and no signs of neurotoxicity including hind-limb paralysis, vocalization, food intake, or neuroanatomical damage were observed in preliminary study."(see pg. 10); behavioral testing (morris water maze); tissue perfusion (dorsal third ventricle);"Our study using this approach of pump-mediated siRNA infusion is efficient in downregulation of NLRP1 mRNA (by about 60%) and protein (by about 50%) levels in APP/PS1 brain. And the treatment with control siRNA did not alter cerebral NLRP1 mRNA and protein levels compared with No siRNA-treated APP/PS1 mice, excluding an effect of pump-mediated infusion on NLRP1 expression levels."pg 8;.

14411 Song, J., Cheon, S.Y., Lee, W.T., Park, K.A., Lee, J.E., Lee, J.E. The effect of ASK1 on vascular permeability and edema formation in cerebral ischemia. Brain Research 2015; 1595(--):143-155. >>> RNA, ASK1-small interfering; siPORTNeoFX; CSF/CNS; Mice; Controls received mp w/ scrambled si-RNA; animal info (male, C57BL6, 8-12 weeks old); ischemia (transient focal cerebral);.

14646 Tan, C.C., Zhang, J.G., Tan, M.S., Chen, H., Meng, D.W., Jiang, T., Meng, X.F., Li, Y., Sun, Z., Li, M.M., Yu, J.T., Tan, L., Yu, J.T. NLRP1 inflammasome is activated in patients with medial temporal lobe epilepsy and contributes to neuronal pyroptosis in amygdala kindling-induced rat model. Journal of Neuroinflammation 2015; 12(--):U1-U12. >>> RNA, small interfering; CSF/CNS (third ventricle); Rat; 2006; Control animals received mp w/ control siRNA in aCSF; animal info (Sprague Dawley, 260-300 g); long-term study;.

14640 Meng, X.F., Tan, L., Tan, M.S., Jiang, T., Tan, C.C., Li, M.M., Wang, H.F., Yu, J.T., Tan, L. Inhibition of the NLRP3 inflammasome provides neuroprotection in rats following amygdala kindling-induced status epilepticus. Journal of Neuroinflammation 2014; 11(--):U1-U12. >>> RNA, small interfering; CSF/CNS (third ventricle); Rat; 2006; Control animals received mp w/ aCSF; animal info (adult, male, Sprague Dawley, 260-300 g); long-term study;.

13927 Sehgal, A., Chen, Q.M., Gibbings, D., Sah, D.W.Y., Bumcrot, D. Tissue-specific gene silencing monitored in circulating RNA. RNA-A PUBLICATION OF THE RNA SOCIETY 2014; 20(-2-):143-149. >>> RNA, small interfering; PBS; CSF/CNS (striatum); Rat; Animal info (male, Sprague Dawley); neurodegenerative (Parkinson's disease); tissue perfusion (striatum); gene therapy; used Plastics One 30g cannula; primed overnight in 37C saline;.

13882 Niimi, N., Kohyama, K., Matsumoto, Y. Minocycline suppresses experimental autoimmune encephalomyelitis by increasing tissue inhibitors of metalloproteinases. NEUROPATHOLOGY 2013; 33(-6-):612-620. >>> RNA, small interfering; IP; Rat; 2002; 2004; Animal info (Lewis, 8-12 weeks old, experimental autoimmune encephalomyelitis); neurodegenerative (multiple sclerosis); gene therapy; immunology;.

13851 Loh, K.P., Ng, G., Yu, C.Y., Fhu, C., Yu, D.J., Vennekens, R., Nilius, B., Soong, T.W., Liao, P. TRPM4 inhibition promotes angiogenesis after ischemic stroke. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2014; 466(-3-):563-576. >>> RNA, small interfering TRPM inhibiting; IV (jugular); Rat; Controls received mp w/ vehicle; animal info (male, Wistar, 300g); ischemia (cerebral); behavioral testing (rotarod apparatus); gene therapy; cardiovascular; TRPM4 aka transient receptor potential melastatin 4;.

13821 Jiang, T., Yu, J.T., Zhu, X.C., Tan, M.S., Gu, L.Z., Zhang, Y.D., Tan, L., Tan, L. Triggering receptor expressed on myeloid cells 2 knockdown exacerbates aging-related neuroinflammation and cognitive deficiency in senescence-accelerated mouse prone 8 mice. NEUROBIOLOGY OF AGING 2014; 35(--):1243-1251. >>> RNA, small interfering TREM2; Cerebrospinal fluid, artifical; CSF/CNS (dorsal third ventricle); Mice; 1004; Controls received mp w/ vehicle or control siRNA; animal info (SAMP8, 6 months old); functionality of mp verified by decrease TREM2 protein levels pg. 1246; ALZET brain infusion kit used; neurodegenerative (Alzheimers); no stress (see pg. 1244); behavioral testing (Morris water maze); tissue perfusion (third ventricle); immunology;.

13705 Armando, I., Villar, V.M., Jones, J.E., Lee, H., Wang, X.Y., Asico, L.D., Yu, P.Y., Yang, J., Escano, CS Jr, Pascua-Crusan, A.M., Felder, R.A., Jose, P.A., Villar, V.M. Dopamine D3 receptor inhibits the ubiquitin-specific peptidase 48 to promote NHE3 degradation. FASEB Journal 2014; 28(--):1422-1434. >>> GR103691; RNA, small interfering; Transfection reagent (TransIT); SC; kidney (subcapsular space); Mice; 1007D; Controls received mp w/ vehicle or nonsilencing"mock siRNA"; animal info (male, C57BL6J, adult); tissue perfusion (subcapsular space; kidney); gene therapy; antihypertensive; GR103691 is a D3R antagonist; used PE tubing #0007701; pump sutured to abdominal wall; surgical glue applied at puncture site to hold catheter tubing in place and to prevent leakage;.

13660 Tan, M.S., Yu, J.T., Jiang, T., Zhu, X.C., Guan, H.S., Tan, L. IL12/23 p40 Inhibition Ameliorates Alzheimer's Disease-Associated Neuropathology and Spatial Memory in SAMP8 Mice. JOURNAL OF ALZHEIMERS DISEASE 2014; 38(-3-):633-646. >>> RNA, small interfering p40; Cerebrospinal fluid, artificial; CSF/CNS (third ventricle); Mice; 1004; Controls received mp w/ control siRNA, aCSF, control antibody; animal info (male, SAMP8, SAMR1, 3 month, 7 month and 11 month old); functionality of mp verified by increased expression of IL-12 and IL-23; ALZET brain infusion kit 3 used; neurodegenerative (Alzheimer); behavioral testing (Morris water maze);.

13635 Cho, K.J., Kim, H.W., Cheon, S.Y., Lee, J.E., Kim, G.W. Apoptosis signal-regulating kinase-1 aggravates ROS-mediated striatal degeneration in 3-nitropropionic acid-infused mice. Biochemical and Biophysical Research Communications 2013; 441(-2-):280-285. >>> RNA, small interfering, ASK1; Nitropropanoic acid, 3-; Saline; CSF/CNS (striatum); SC; Mice (transgenic); 1007D; Controls received mp w/ vehicle; animal info (male, SOD1-tg); functionality of mp verified by Western blotting; Multiple pumps per animal (2); neurodegenerative (apoptotic striatal degeneration); behavioral testing (rotarod test); tissue perfusion (striatum); 3-NP delivered SC, ASK1-siRNA delivered CSF/CNS.

13626 Song, J., Cho, K.J., Cheon, S.Y., Kim, S.H., Park, K.A., Lee, W.T., Lee, J.E. Apoptosis signal-regulating kinase 1 (ASK1) is linked to neural stem cell differentiation after ischemic brain injury. EXPERIMENTAL AND MOLECULAR MEDICINE 2013; 45(-;-):U28-U34. >>> RNA, Apoptosis signaling kinase 1-small interfering; Transfection medium, siPORT NeoFX; CSF/CNS; Mice; Controls received mp w/ vehicle and scrambled siRNA (negative control) or siRNA agains glyceraldehyde 3-phosphate dehydrogenase (positive control); animal info (male, C57BL6, 8-12 weeks old); ischemia (transient focal cerebral); immunology;.

13554 Ng, O.T.W., Chen, L.W., Chan, Y.S., Yung, K.K.L. Small Interfering RNA Specific for N-Methyl-D-Aspartate Receptor 2B Offers Neuroprotection to Dopamine Neurons through Activation of MAP Kinase. NEUROSIGNALS 2013; 21(-1-2-):42-54. >>> RNA, small interfering; Saline, SilentFect; CSF/CNS (striatum); Rat; 2001; Animal info (male, Sprague Dawley, 200-250g); ALZET brain infusion kit 2 used; comparison of single injection vs mp"In summary, single injection of siRNA into PD models showed no significant effect in ameliorating the motor syndrome and protection in TH-positive neurons. However, continuous infusion of NR2B-specific siRNA can effectively ameliorate the motor symptoms, attenuate the dopaminergic cell loss in the striatum and SN regions, and promote the ERK1/2 signaling pathway in parkinsonian models."pg53; neurodegenerative (Parkinson's disease);"Moreover, by using the osmotic minipump connecting with canals, this method can be easily transplanted into patients, and allow the siRNA to be infused to the target site directly at a steady and slow rate. The direct delivery has the advantages of reducing any undesired systemic side effects and lowering the dose requirement for efficacy"pg 53 ; Primed at 37C saline overnight.

13481 Briones, T.L., Woods, J., Wadowska, M. Chronic neuroinflammation and cognitive impairment following transient global cerebral ischemia: role of fractalkine/CX3CR1 signaling. Journal of Neuroinflammation 2014; 11(-;-):U1-U13. >>> RNA, small interfering CX3CR1; RNA, scrambled; Transfection medium; CSF/CNS; Rat; 1002; Animal info (male, Wistar, 350-375g); ALZET brain infusion kit 2 used; ischemia (cerebral); post op. care (heating pad); behavioral testing (Morris water maze); gene therapy; immunology; Cannula stabilized using dental cement. Pumps primed in sterile saline 37C overnight..

13469 Pignataro, G., Esposito, E., Sirabella, R., Vinciguerra, A., Cuomo, O., Di Renzo, G., Annunziato, L. nNOS and p-ERK involvement in the neuroprotection exerted by remote postconditioning in rats subjected to transient middle cerebral artery occlusion. NEUROBIOLOGY OF DISEASE 2013; 54(-;-):105-114. >>> RNA, small interfering, nNOS blocking; CSF/CNS; Rat; Controls received sham surgery, no ischemia and control siRNA (non nNOS blocking); animal info (male, Sprague Dawley, 250-300g); ischemia (cardiac; femoral artery);.

13248 Villar, V.A.M., Armando, I., Sanada, H., Frazer, L.C., Russo, C.M., Notario, P.M., Lee, H., Comisky, L., Russell, H.A., Yang, Y., Jurgens, J.A., Jose, P.A., Jones, J.E. Novel role of sorting nexin 5 in renal D(1) dopamine receptor trafficking and function: implications for hypertension. FASEB Journal 2013; 27(-5-):1808-1819. >>> Ringer's solution, lactated; RNA, small interfering, Snx-5, non-silencing; SC; Rat; Controls received mp w/vehicle or (non-silencing mock siRNA); animal info (spontaneously hypertensive rats (SHR)); antihypertensive; mp used to infuse sorting nexin 5 (SNX5). It decreases sodium excretion, and elevtes systolic blood pressure. SNX5 regulated D1 receptor, which has an important therapeutic implication in the management of essential hypertension;.

13117 Yang, Y., Zhang, Y.R., Cuevas, S., Villar, V., Escano, C., Asico, L.D., Yu, P.Y., Grandy, D.K., Felder, R.A., Armando, I., Jose, P.A. Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D(2) receptor-induced inhibition of NADPH oxidase. Free Radical Biology and Medicine 2012; 53(-3-):437-446. >>> RNA, small interfering, D2R; RNA, small interfering, PON2; Kidney (renal capsule); Mice; 1007D; Control animals received mp w/ nonsilencing control RNA; animal info (D2R deficient, 6-8 mo old, uniphrectomy); PE tubing used (item #0007701);"The body of the minipump was placed in the area previously occupied by the kidney that was removed; stabilization was achieved by suturing (4-0 ethilon) the minipump to the lateral abdominal musculature close to it."; tissue perfusion (renal capsule).

13017 Yu, D.B., Pendergraff, H., Liu, J., Kordasiewicz, H.B., Cleveland, D.W., Swayze, E.E., Lima, W.F., Crooke, S.T., Prakash, T.P., Corey, D.R. Single-Stranded RNAs Use RNAi to Potently and Allele-Selectively Inhibit Mutant Huntingtin Expression. Cell 2012; 150(-5-):895-908. >>> RNA, small interfering, single stranded; PBS, sterile; CSF/CNS; Mice; 2002; 2004; Control animals received mp w/ vehicle; animal info (HdhQ150); cyanoacrylate adhesive used; neurodegenerative (Huntington's disease).

12982 Matsumoto, Y., Niimi, N., Kohyama, K. Characterization of fibrosis-promoting factors and siRNA-mediated therapies in C-protein-induced experimental autoimmune myocarditis. Cellular Immunology 2012; 279(-1-):70-77. >>> RNA, small interfering, MMP-2; RNA, small interfering, MMP-9; IP; Rat; 2004; Animal info (male, female, 8-12 wks old, Lewis).

12962 Tao, F., Li, Q., Liu, S., Wu, H.Y., Skinner, J., Hurtado, A., Belegu, V., Furmanski, O., Yang, Y., McDonald, J.W., Johns, R.A. Role of Neuregulin-1/ErbB Signaling in Stem Cell Therapy for Spinal Cord Injury-Induced Chronic Neuropathic Pain. Stem Cells 2013; 31(-1-):83-91. >>> RNA, small interfering; CSF/CNS (intrathecal); Rat; 2004; Control animals received mp w/ non target RNA; animal info (Long Evans, female, 250 g); NRG1 siRNA; spinal cord injury.

12924 Kesanakurti, D., Chetty, C., Dinh, D.H., Gujrati, M., Rao, J.S. Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with alpha5beta1 integrin in glioma. ONCOGENE 2013; 32(-3-):327-340. >>> Plasmid, scrambled vector; RNA, small interfering; Mice (nude); 2004; Animal info (nu/nu, 4-6 wks old, athymic); MMP-2 siRNA.

12757 Villar, V.M., Jones, J.E., Armando, I., Asico, L.D., Escano, CS Jr, Lee, H., Wang, X.Y., Yang, Y., Pascua-Crusan, A.M., Palmes-Saloma, C.P., Felder, R.A., Jose, P.A. Sorting Nexin 1 Loss Results in D(5) Dopamine Receptor Dysfunction in Human Renal Proximal Tubule Cells and Hypertension in Mice. Journal of Biological Chemistry 2013; 288(-1-):152-163. >>> RNA, small interfering; Kidney; Mice; Control animals received mp w/ vehicle or non-silencing mock siRNA; animal info (C57BL/6, BALB/c, nephrectomized, adult, male); Snx-1 specific siRNA; infusion rate of 0.5 ul/hr; tissue perfusion (kidney); PE catheter used.

12453 Dyck, B.A., Tan, M.L., Daya, R.P., Basu, D., Sookram, C.D.R., Thomas, N., Mishra, R.K. Behavioral effects of non-viral mediated RNA interference of synapsin II in the medial prefrontal cortex of the rat. SCHIZOPHRENIA RESEARCH 2012; 137(-1-3-):32-38. >>> RNA, small interfering; Water, sterile; CSF/CNS (medial prefrontal cortex); Rat; 2002; Controls received mp w/ artificial CSF; animal info (Sprague Dawley, male, 250-300 g); bilateral infusion using vinyl tubing and two Plastics One cannulae; schematic of pump plus cannula (fig 5); cocktail of 4 synapsin II siRNAs.

12358 Reufsteck, C., Lifshitz-Shovali, R., Zepp, M., Baeuerle, T., Kuebler, D., Golomb, G., Berger, M.R. Silencing of skeletal metastasis-associated genes impairs migration of breast cancer cells and reduces osteolytic bone lesions. CLINICAL&EXPERIMENTAL METASTASIS 2012; 29(-5-):441-456. >>> RNA, small interfering, bone sialoprotein; RNA, small, interfering, osteopontin; SC; Rat (nude); Controls received mp w/ nonsense control siRNA; animal info (male, nu/nu, 6-8 wks old); pumps replaced after 2 weeks; cancer.

12311 Zhang, Y.R., Cuevas, S., Asico, L.D., Escano, C., Yang, Y., Pascua, A.M., Wang, X.Y., Jones, J.E., Grandy, D., Eisner, G., Jose, P.A., Armando, I. Deficient Dopamine D2 Receptor Function Causes Renal Inflammation Independently of High Blood Pressure. PLoS One 2012; 7(-6-):U180-U190. >>> Apocynin; RNA, small interfering, Drd2; SC; IA (intrarenal); Mice; 1007D; Controls received mp w/ vehicle; animal info (adult, male, D2 receptor deficient, uniphrectomy); enzyme inhibitor (NADPH oxidase);"Surgical glue was applied at the puncture site to hold the tubing in place and prevent extra-renal leakage. The osmotic pump was sutured to the abdominal wall to prevent excessive movement of the pump for the duration of the study."pg e38745; incorrectly listed ALZET Catheter 0007701 as PE tubing (polyurethane).

12174 Cuevas, S., Zhang, Y.R., Yang, Y., Escano, C., Asico, L., Jones, J.E., Armando, I., Jose, P.A. Role of Renal DJ-1 in the Pathogenesis of Hypertension Associated With Increased Reactive Oxygen Species Production. Hypertension 2012; 59(-2-):446-452. >>> RNA, small interfering, DJ-1; In vivo transfection agent; Kidney; Mice; 1007D; Controls received mp w/ non-silencing RNA; animal info (F1 hybrid; wt, 6-8 mo old, uninephrectomized, adult, male, C57BL/6J); ALZET polyethylene tubing set used (0007701); tissue perfusion (kidney);"The osmotic pump was sutured to the abdominal wall to prevent excessive movement of the pump"pg 447.

 

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What researchers are saying...

“This study demonstrates the value of osmotic pumps as effective delivery vehicles for drugs in wild demersal fish.” (p.1024). “We conclude that the administration of T3 by osmotic pumps is an effective means of creating experimental hyperthyroidism in wild Atlantic cod.Similarly, the administration of methimazole, thiourea, or testosterone with osmotic pumps was effective in rendering cod hypothyroid.” (p.1025) Comeau et al., Transactions of the American Fisheries Society 2003;132:1021-1026.