ALZET Accessories

When using ALZET osmotic pumps for various targeted delivery applications, special catheter tubing and/or other surgical materials may be needed. A list of additional accessories and potential vendors is listed below for your convenience.

Catheter Tubing

When performing intravenous, intra-arterial, or other targeted delivery studies with ALZET osmotic pumps, you may need catheter tubing of various requirements. The appropriate sizes of polyethylene and vinyl tubing that fit the ALZET osmotic pump flow moderator are listed below. Other types of catheter tubing can also be used.

Polyethylene Tubing

Medical grade polyethylene tubing is available in many sizes from most laboratory supply houses. Two sizes are commonly used with ALZET pumps: PE-50 and PE-60. However, the larger size (PE-60) is generally preferred. DURECT Corporation sells sterile catheter tubing (PE-60), pre-cut to 15 cm lengths, and individually packaged.

 

Tubing type
I.D.
O.D.
Supplier
Order Number
PE-60

0.030 in.

(0.76 mm)

0.048 in.

(1.14 mm)

DURECT Corp

Order Now

0007750
PE-50

0.023 in.

(0.58 mm)

0.038 in.

(0.99 mm)

Scientific Commodities Inc. BB31695-PE/3

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Vinyl Tubing

Medical grade vinyl tubing has superior flexibility and attachment properties (compared to PE tubing), hence it may be better suited for some ICV applications. DURECT Corporation sells sterile catheter tubing (vynil), pre-cut to 15 cm lengths, and individually packaged.

 

Size I.D. O.D. Supplier Order Number
V/3

0.023 in.

(0.58 mm)

0.039 in.

(0.99 mm)

Scientific Commodities Inc. BB31785-V/3
V/3A

0.027 in.

(0.69 mm)

0.045 in.

(1.14 mm)

DURECT Corp

Order Now

0007760
V/4

0.030 in.

(0.76 mm)

0.048 in.

(1.22 mm)

Scientific Commodities Inc. BB31785-V/4

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Intra-arterial / Intravenous Applications

For intra-arterial or intravenous applications in small vessels, refer to our line of specialized catheters. These catheters are customized for delivery to small vessels and attachment to the ALZET pump. Alternatively, PE-60 tubing can be drawn out over a heat source to a very small size. Furthermore, very fine polyethylene tubing can be glued inside PE-60. One example of such fine tubing is Intramedic® tubing, available from:

Scientific Commodities Inc.
2800 Sweetwater Ave. Suite A-105
Lake Havasu City, AZ 86406
(800) 331-7724
(928) 855-0150 (fax)
e-mail: sales@scicominc.com

Fisher Scientific
P.O. Box 58056, Santa Clara, CA 95050
(800) 766-7000
(800) 926-1166 (fax)
(408) 727-4905 (fax)

 

Size I.D. O.D. SCI Catalog Number Fisher Catalog Number
PE-10

0.011 in.

(0.28 mm)

0.025 in.

(0.64 mm)

BB31695-PE/1 14-170-12P

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Handbook of Pre-clinical Continuous Intravenous Infusion

Edited by Guy Healing and David Smith, Taylor & Francis, 2000.

This book provides an excellent overview of surgical procedures, equipment, and practical tips for conducting intravenous infusion studies in various laboratory animal species.  Researchers across multiple scientific disciplines will find that this handbook contains the information and guidance necessary to conduct more efficient and accurate studies.  An entire chapter from this book (Chapter 21) is dedicated to "The use of ALZET mini-pumps in continuous infusion studies".  This chapter offers an excellent overview on the use of ALZET mini-pumps in pre-clinical pharmacology and toxicology studies. It also offers detailed surgical procedures and tips for jugular vessel cannulation in rodents.  This book is available from Braintree Scientific Inc. and Amazon.com.

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Brain Infusion Studies

For brain infusion studies in rats or mice, DURECT offers the ALZET Brain Infusion Kit. This kit contains infusion cannulae and vinyl catheter tubing for ten brain infusion studies. Additional materials useful in brain infusion studies are available from the following vendors:

 

Small Parts, Inc. (through Amazon Supply)
(800) 220-4242 (toll free)
Contact Amazon Supply

Holes in the skull can be drilled with a 1-3/4 " length, 0.0465" cutting diameter, precision steel, high speed steel bit such as part number: (B0025QE8A0) and a small 0.030"-0.062" pin vice (part number: B001LF1156).

Small stainless steel machine screws (size #0-80, 1/8" in length with a fillister head, part number: (B000FN29YM) can be used to stabilize the dental cement platform.

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Dental Cement

Dental cement can be used to keep the brain infusion cannula in place. Dental cement is available through the following vendors:

 

 

Henry Schein, Inc.    (Catalog number 125-1613)
Veterinary Division, 135 Duryea Road, Melville, NY 11747
(800) 552-8387, or (516) 843-5325
(516) 843-5676 (fax)
e-mail: kguinn@henryschein.com

Stoelting Co.    (Product number 51458, or 51459)
620 Wheat Lane, Wood Dale, IL 60191
(630) 860-9700
(630) 860-9775 (fax)
e-mail: physiology@stoeltingco.com

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Cyanoacrylate Adhesive (Loctite 454)

Cyanoacrylate gel is an adhesion compound which can be used in lieu of dental cement to keep the brain infusion cannula in place. It is particularly useful for mouse applications. Loctite Prism¨ 454 is one example of a cyanoacrylate gel that can be used for securing the brain cannulae to the skull of laboratory animals. This product can be purchased directly from:

DURECT Corporation (Product Number 0008670) Order Now
PO Box 530 Cupertino, CA 95015-0530
(877) 922-5938
(408) 865-1406 (fax)
e-mail: alzetcs@durect.com

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Cannula Holder

Now available from DURECT Corporation - These stainless steel devices facilitate stereotaxic placement of brain infusion cannula to the skull. They are specifically designed to fit the removable cannula tab, and they are compatible with most of the common stereotaxic frames in the market.

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Custom Infusion Cannulae

Plastics One manufactures cannulae that can be used with ALZET osmotic pumps. These can be custom ordered to specific lengths and gauges from:

Plastics One
6591 Merriman Road SW, Roanoke, VA 24018
(540) 772-1133 (Phone)
(540) 777-5900 (fax)
e-mail: ashifflett@plastics1.com

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Stereotaxic Atlases and Related References

A compilation of references to stereotaxic atlases and related materials is available through the David Kopf Instruments web site. These listings provide investigators reference to a variety of stereotaxic atlases, and other ancillary techniques and methods related to stereotaxic surgery in various animal species.

Stereotaxic Atlases can also be purchased from:

Braintree Scientific, Inc.
P.O. Box 850929
Braintree, MA 02185
(781) 843-2202
e-mail: info@braintreesci.com

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Artificial CSF

For infusions into the brain (either via the cerebral ventricles or into solid tissue), the preferred vehicle is artificial cerebrospinal fluid (CSF). A recipe for preparing artificial CSF is available (click here). Commercial source of Artificial CSF Perfusion Fluid: Harvard Apparatus

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Blue Dye

ALZET pumps are supplied with a specified volume release rate that has been determined in isotonic saline at 37C (+/- 0.5C). In the event that the user finds it necessary to test the pumping rate, DURECT has developed an in vitro testing protocol. This protocol requires the use of an indicator solution. One such indicator is FD&C #1 blue dye (catalog #5601), available from:

Sensient Color Group
2515 N. Jefferson Ave.,
St. Louis, MO 63106
(800) 325-8110 x5001

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Bacterial Filters

Bacterial filters that attach to the filling syringe may be used to prevent particulate contamination during loading of test solutions. Suitable filters (i.e., Millex®-GV - 0.22 µM syringe-end filter) are available from Millipore Corporation